Introduction: Grape (Vitis vinifera L.) is one of the largest fruit crop widely grown throughout the world and their seeds are considered a relevant source of polyphenol compounds. Objective: To carry out phytochemical and pharmacognostic evaluation of the seeds of Vitis vinifera L. Var sahibi and hussaini, family vitiaceae as per WHO guidelines. Materials and Methods: In present investigation, the detailed pharmacognostic studies were carried out in terms of organoleptic properties, macroscopical, microscopical characters, physicochemical parameters, preliminary phytochemical screening for major groups of compounds and other WHO recommended parameters for standardization of the seeds of Vitis vinifera L. Var. sahibi and hussaini. Results: Macro and microscopic characters of Vitis vinifera L. seeds Var. sahibi and hussaini were analyzed. Physicochemical parameters such as foreign matter, extractive values, ash content, pH and fluorescent behaviour of seed powder were done by using WHO recommended parameters. A preliminary phytochemical screening was done and revealed the presence of different phytoconstituents like carbohydrates, flavonoids, glycosides, tannins, sterols and fixed oil. Conclusion: This is the first report of its kind on the pharmacognostic studies of Vitis vinifera L. seeds Var. sahibi and hussaini and will serve as valuable source of information towards establishing pharmacognostic standards on identification, purity, quality and classification.