Introduction: Plants are a rich source of bioactive compounds. Simmondsia chinensis, also known as Jojoba, is the sole member the Simmondsiaceae’s family and has been known traditionally for many medical uses. Objectives: Herein we evaluate the value of crude jojoba oil (J.O) as an antimicrobial agent in vitro. Methods: J.O was tested for potential antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, P. mirabilis, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Asperigillus flavus. Results: Our results did not show any effect on fungi or yeast. However, a significant antibacterial activity was observed against B. subtilis, S. aureus, P. vulgaris, P. mirabilis. A high activity was observed for J.O at Minimum inhibitory concentration (MIC) level of 12.5 mg/ml. Interestingly, S. typhimurium, E. coli and Ps. aeruginosa were found to be highly resistant. Conclusion: Our findings suggest that J.O may have a medicinal potential as natural antibacterial agent.