@article {1967, title = {Alkaloids from Pandanus amaryllifolius Roxb Leaf as Promising Candidates for Antidyslipidemic Agents: An in silico study}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {March 2023}, pages = {106-111}, type = {Original Article }, chapter = {106}, abstract = {

Introduction: The plant Pandanus amaryllifolius Roxb (pandan), has been shown to have antidyslipidemic potency. This study explored the potential of several alkaloids from pandan leaf as antidyslipidemia as well as their safety profile in silico. Methods: Analyses were carried out by studying the binding affinity of the alkaloids to 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, peroxisome proliferator activator receptor (PPAR) alpha and Niemann Pick C1 Like 1 (NPC1L1). The structures of the alkaloids were downloaded from the Pubchem database and optimized using the ChemDraw Professional 16.0 to obtain 3D structures in protein data bank (PDB) format. The in silico testing was based on the interactions of the alkaloids with the HMG-CoA reductase (PDB ID 1HW9), PPAR alpha (PDB ID 6LX4) and NPC1L1 (PDB ID 7DFZ) proteins, downloaded from the Research Collaboratory for Structural Bioinformatics (RSCB) PDB website (http://www.rcsb.org/pdb). The preparation of protein structures was performed using the Discovery studio 2021 client and Gromacs applications, while optimization of the 3D structure of the alkaloids was carried out with the ChemDraw professional 16.0. Finally, validation was completed using AutoDock application. The safety profile was assessed by pkCSM online tool. Results: The respective root mean square deviation (RMSD) values of the 1HW9, 6LX4 and 7DFZ proteins were 1.677, 0.918 and 1.706, respectively. The alkaloids pandanusine B, pandamarilactonine A, pandamarilactonine B had respective values of binding energy for HMG-CoA of -5.52, -5.51 and -5.46 kcal/mol. The binding energy of pandamarilactonine B, pandamarilactonine A and pandanamine for PPAR alpha were -9.14, -9.10 and -8.48 kcal/mol, respectively, with the corresponding energy for t NPC1L1 of -9.63, -9.71 and -8.54 kcal/mol. The toxicity tests indicated that the alkaloids were safe, pandamarilactonines had the highest LD50 (2.736 mol/ kg). Conclusion: The studied pandan alkaloids have potential antidyslipidemic activity by interacting with HMG-CoA reductase, PPAR alpha, and NPC1L1, with good safety profile.

}, keywords = {Alkaloids, Dyslipidemia, In Silico., Pandan, Pandanus amaryllifolius}, doi = {10.5530/pj.2023.15.14}, author = {Martohap Parotua Lumbanraja and Kusnandar Anggadiredja and Hubbi Nashrullah Muhammad and Neng Fisheri Kurniati} } @article {758, title = {Alpha Mangostin and Xanthone Activity on Fasting Blood Glucose, Insulin and Langerhans Islet of Langerhans in Alloxan Induced Diabetic Mice}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {January 2019}, pages = {64-68}, type = {Original Article}, chapter = {64}, abstract = {

Objective: This research elaborated role of alpha mangostin and xanthone on fasting blood glucose, insulin and langerhans islet in alloxan induced diabetic mice. Methods: Fasting blood glucose, insulin and langerhans islet test were conducted using male Mus musculus mice, divided into 10 groups randomly, which were normal, control (alloxan induced only), glibenclamide, various doses of α-mangostin and xanthone (5, 10, 20 mg/kgbw). Mice were treated for 21 days. Overnight-fasted mice (12 h) were sacrificed by cervical decapitation on day 21st, following the ethical norms granted by the ethics committee. Fasting blood glucose and insulin plasma were checked. Pancreatic tissues were excised from sacrificed animals, and then fixed in 10 \% (v/v) neutral buffered formalin. Histologic observations for Langerhans area were performed after staining using Gomori staining method. Results: The effects of alpha mangostin and xanthone on fasting blood glucose different significantly to control, and were not significantly different from glibenclamide and metformin. Increasing alpha mangostin/xanthone dose from 5 mg/kgbw to 20 mg/bw also did not cause significant differences, although the best results were obtained at a dose of 20 mg/kgbw. Insulin plasma analize showed that there were no significant difference between alpha mangostin/xanthone to normal group, except xanthone 10 mg/kgbw. Langerhans area showed significant difference between alpha mangostin/xanthone to control group. But there{\textquoteright}s still had significant difference if we compare to glibenclamid/metformin group. Conclusion: Alpha mangostin and xanthone are two substances that showed antidiabetic effect on fasting blood glucose level, insulin plasma and Langerhans islet.

}, keywords = {Fasting blood glucose, Insulin plasma, Langerhans, Xanthone, α-mangostin}, doi = {10.5530/pj.2019.1.12}, author = {Welly Ratwita and Elin Yulinah Sukandar and I Ketut Adnyana and Neng Fisheri Kurniati} }