@article {557, title = {Potential Bioactive Compounds Isolated from Boesenbergia rotunda as Antioxidant and Antimicrobial Agents}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {513-518}, type = {Original Article}, chapter = {513}, abstract = {

Objective: This study was conducted to identify some bioactive compounds of Boesenbergia rotunda rhizome and to test as antioxidant and antimicrobial agents. Methods: The milled dried rhizome of B. Rotunda (5kg) was extracted exhaustively with ethanol. The ethanol extract was partitioned three times by n-hexane, chloroform, and ethyl acetate respectively. Each fraction was fractionated by vacuum liquid chromatography (VLC) and then purified by column chromatography gravitation. Structural identification of all pure compounds were elucidated based on spectroscopic methods (UV, IR, and NMR).The antioxidant activity was tested by 2,2-diphenyl- 1-picrylhydrazyl (DPPH). Antimicrobial character was screened for activities againts pathogenic bacteria i.e. Escherichia coli ATCC-11229, Staphylococcus aureus ATCC-25923, Staphylococcus epidermidis FNCC-0048, and Streptococcus mutans by the disk-diffusion method. The assay was done in triplicate, and chloramphenicol was used as the positive control. Results and Discussion: From ethanol extract of B. rotunda three known compounds of flavanones, namely 2\’,4\’-dihydroxy-6-methoxychalcone (compound-1), 5-hydroxy-7- methoxyflavanone (compound-2), and 5,7-dihydroxyflavanone (compound-3) were isolated. The ethanol extract of B. rotunda and the three isolated compounds -1,-2, and -3 showed an antioxidant activity with the IC value of 92.64; 46.66; 62.84, and 62.66 \μg/mL, respectively. The zone of inhibition of extract and the three isolated compounds showed moderate activity against Escherichia coli ATCC-11229, Staphylococcus aureus ATCC-25923, Staphylococcus epidermidis FNCC-0048, and Streptococcus mutans.The maximum zone of inhibition was 13.20\±0.76 mm at the maximum concentration used (500 \μg/mL) against Escherichia coli ATCC-11229, and the minimum inhibitory concentration(MIC) for each bacteria was found to be 0.5\μg/mL. Conclusion: The result of the study suggests that B. rotunda rhizome contains potential bioactive compounds which could be suitable for antioxidant and the treatment of various infections caused by Escherichia coli ATCC-11229, Staphylococcus aureus ATCC- 25923, Staphylococcus epidermidis FNCC-0048, and Streptococcus mutans.

}, keywords = {Antimicrobial, Antioxidant, Bioactive compound, Boesenbergia rotunda}, doi = {10.5530/pj.2018.3.84}, url = {http://fulltxt.org/article/517}, author = {Sri Atun and Sri Handayani and Anna Rakhmawati} } @article {242, title = {Synthesis of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid and its Biological Activity test}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {142-147}, type = {Original Article}, chapter = {142}, abstract = {

Introduction: B. rotunda used as a traditional medicine to treat illnesses such as rheumatism, muscle pain, febrifuge, gout, gastrointestinal disorders, flatulence, carminative, stomach ache, dyspepsia, and peptic ulcer. To enhance the stability, solubility and activity of the extract B. rotunda, should be developed into a product nanoparticles. Objective: This work reports the synthesis of nanoparticles produced by ethanol extract of Boesenbergia rotunda rhizome loaded with chitosan and alginic acid, and its biological activity test as antioxidant. Method: The synthesis of nanoparticles used an ionic gelation. Activity of the nanoparticle products as antioxidant was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Results: This work showed that nanoparticles chitosan produced by ethanol extract B. rotunda can be synthesized at a concentration (\% w/v) of chitosan/ Na-TPP (sodium tripolyphosphate) and ratio of 8: 1, the size range of the nanoparticles were 389 to 877 nm, with a zeta potential of + 41.87 mV, and precentage nanoparticle 98.1\%. The corresponding nanoparticles alginic acid can be synthesized at a concentration (\% w/v) of alginic acid/ Ca2+ and ratio of 5: 1, the size range of the nanoparticles were 197 to 877 nm, with a zeta potential of -82.1 mV, and precentage nanoparticle 90.2\%. Furthermore, nanoparticles result of the combination of alginic acid \–chitosan can be synthesized at a concentration (\% w/v) alginic acid/chitosan/ca2+ and ratio of 6.7: 2: 1, the size range of the nanoparticle were 226 to 877 nm, and precentage nanoparticle 29.7\%. The morphology of each nanoparticle products was spherical and a smooth surface. The chitosan-alginic acid nanoparticles show higher activity than the starting material ethanol extract of B. rotunda.

}, keywords = {alginic acid, Antioxidant, B. rotunda, chitosan-alginic acid combination, Nanoparticles chitosan}, doi = {10.5530/pj.2017.2.24}, url = {http://phcogj.com/fulltext/291}, author = {Sri Atun and Sri Handayani} }