@article {2089, title = {RETRACTED: The Effects of Andrographolide on Apoptosis in PC-3 Cell Line Via the Involvement of Caspases 3, 8 And 9}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {August 2023}, pages = {612-621}, type = {Research Article}, chapter = {612}, abstract = {

The Article has been Retracted based on the Authors{\textquoteright} Request.

}, keywords = {Andrographolide, Anti-cancer, Apoptosis, Caspase., PC-3 cell line}, doi = {10.5530/pj.2023.15.128}, author = {Janany Manimaran and Daruliza Kernain Mohd Azman} } @article {1409, title = {Isolation of Andrographolide from Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke and its Anticancer Activity against Human Ovarian Teratocarcinoma}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {660-668}, type = {Original Article}, chapter = {660}, abstract = {

Introduction: Andrographolide is a well-known anticancer phytochemical often isolated from Andrographis paniculata (Burm. f.) Nees. (Acanthaceae). Though Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke (ALw) which also belongs to the same family has an adequate amount of andrographolide; remained untouched for isolation of andrographolide and anticancer studies. Therefore, this study was targeted to isolate the andrographolide from the leaves of ALw and to assess its role inthe induction of apoptosis against the human ovarian teratocarcinoma (PA-1) cell line. Methods: Column chromatography, thin-layer chromatography (TLC), preparative TLC were used for the isolation and purification while melting point, ultraviolet (UV)-visible spectroscopy, Fourier transform infrared (FTIR), proton nuclear magnetic resonance (1H NMR), carbon-13 (C13) nuclear magnetic resonance (13C NMR) analysis were carried out for characterization of the compound. 3-(4,5-dimethylthiaxo-2yl) 2, 5-diphenyl tetrazolium bromide (MTT) assay was carried out for cytotoxicity test and further Annexin-V staining, caspase 3 activity, B-cell lymphoma-2 (Bcl-2) activity, cell cycle analysis, and DNA damage study by terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) assays were carried out for apoptosis study. Results: Andrographolide was isolated from the methanolic extract of leaves of ALw which had a melting point of 230 {\textordmasculine}C, λmax at 223 nm. FTIR results proved the presence of hydroxyl group, alkanes, carbon-carbon double bond, and a characteristic gamma lactone carbonyl. NMR data confirmed the 20 carbon structure. In the MTT assay cytotoxicity against PA-1 was at 3.7 μg/ml with other apoptotic assays supporting the induction of apoptosis by the compound at that concentration. Conclusion: ALw is proved to be an alternate source of andrographolide with potential abilities to induce apoptosis in ovarian cancer cells.

}, keywords = {Andrographis, Andrographolide, Anticancer activity, Apoptosis, Ovarian teratocarcinoma}, doi = {10.5530/pj.2021.13.84}, author = {Medha A. Bhat and Hosakatte Niranjana Murthy} } @article {531, title = {Andrographolide Induced Apoptosis in NALM-6 Cells Mediated Through the Cell Cycle Arrest and Nuclear Fragmentation}, journal = {Pharmacog Journal}, volume = {10}, year = {2018}, month = {January-2018}, pages = {210-214}, type = {Original Article}, chapter = {210 }, abstract = {

Introduction: Andrographis paniculata is an herb widely cultivated in South and Southeastern Asia. It has been traditionally used to treat infections and other Physiological disorders for several hundreds. We investigated the anti-leukemic potential of Andrographolide (AGP) isolated from the leaves of this plant against an array of cancer cells to investigate its most efficacies in a particular cancer type. Methods: AGP was isolated from Andrographis paniculata leaves by using column chromatography. The structure was further determined by LC-MS, 1H NMR and 13C NMR. AGP was initially tested against four different cancer cell lines, namely NALM-6 (pre B-ALL), K562 (CML), A549 (lung carcinoma) and MCF-7 (breast carcinoma) using MTT assay at different time points and different concentrations. The effect of the isolated biomolecule was also investigated in inducing apoptosis through the study of cell cycle progression using flow cytometry by PI staining and nuclear fragmentation pattern by DAPI staining and fluorescence microscopy. Results: the spectral analysis of the isolated bio-molecule assured that the compound was AGP. MTT assay data indicated that AGP was most potent to induce cytotoxicity in NALM-6 cells. Further investigation revealed that it effectively induced apoptosis by arresting cell cycle progression and increased the nuclear break down in NALM- 6 leukemic cells. Conclusion: Our study efficiently demonstrated that the AGP isolated from Andrographis paniculata induced apoptosis in NALM-6 cells, which could be used in the therapeutic intervention of leukemia in the future.

}, keywords = {Andrographis paniculata, Andrographolide, Apoptosis, Cell cycle, Cytotoxicity, Leukemia}, doi = {10.5530/pj.2018.2.36}, url = {http://fulltxt.org/article/466}, author = {Swadesh Sarkar and Priya K Gopal and Santanu Paul} }