@article {1408, title = {Preliminary Phytochemical Studies, GC-MS Analysis and In vitro Antioxidant Activity of Selected Medicinal Plants and its Polyherbal Formulation}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {648-659}, type = {Original Article}, chapter = {648}, abstract = {

Background: Novel polyherbal formulation (PHF) is the utilization of more than one herb in the preparation of herbal medication. The thought is found in the conventional system of medicine where the variety of herbs in a specific proportion of illness. Because of synergism, polyherbalism presents a few advantages which aren{\textquoteright}t accessible in single herbal medication. It is utilized in these medications for the treatment of numerous sicknesses including antioxidants. Objective: To develop a phytochemical screening and GC-MS analysis of Novel Polyherbal formulation for In vitro antioxidant activity. Materials and Methods: Macroscopical, preliminary phytochemical, quantitative phytoconstituents, and In-vitro antioxidant activity of all the individual extract and polyherbal formulation was done by chemical method. Identification of phytoconstituents with the aid of Gas chromatography {\textendash} Mass spectroscopy (GC-MS). Results: Macroscopical study and physicochemical examination, for example, ash value, extractive value, loss on drying, and pH were reported to A. racemosus, B. variegata, C. bonducella, S. asoka, and S. racemosus and novel polyherbal formulation. Qualitative phytochemical investigation revealed the presence of alkaloids, flavonoids, gums \& mucilage, carbohydrates, steroids, proteins \& amino acids, fats \& fixed oils, glycoside, phenols, and saponins. Quantitative estimation such as TAC, TFC, TGC, TSC, and TPC was showed positive results. All the individual extract and PHF were subjected to GC-MS analysis. All the individual extract and polyherbal formulation displayed strong antioxidant activity. Conclusions: To conclude the PHF was reported that high level of bioactive contents present and strong antioxidant activity in contrast to the preferred ascorbic acid. The GC-MS uncovered the presence of bioactive compounds and these compounds are suggested to treat antibacterial, antioxidant, anti-inflammatory, and antiviral, anti-tumor, anti-proliferative activity, and antifungal activity.

}, keywords = {Antioxidant, GC-MS analysis, Macroscopical, Phytochemical, Polyherbal formulation}, doi = {10.5530/pj.2021.13.83}, author = {Shalini K and Ilango K} } @article {205, title = {Phytochemical Evaluation of Polyherbal Formulation of Clinacanthus nutans and Elephantopus scaber to Identify Flavonoids}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {September 2016}, pages = {534-541}, type = {Original Article}, chapter = {534}, abstract = {

Background: Modern healthcare system recognizes herbal medicine as a form of alternative medicine and also identify as holistic approach. Everyone in life experiences different kind of wound.Clinacanthus nutans and Elephantopus scaber are well known traditional wound healing herbs. Objective: To develop a new polyherbal formulation in the treatment of wound and identify flavonoid by means of chromatography, chemical method and spectroscopic method. Preliminary phytochemical and fluorescent evaluation of Clinacanthus nutans,Elephantopus scaber and herb-herb combination. Methods and Material: Preliminary phytochemical and fluorescent evaluation of Clinacanthus nutans, Elephantopus and polyherbal formulation of both herb by chemical method. Identification of flavonoids by thin layer chromatography and fourier transform infrared spectroscopy. Results: Flavonoids have found inside polyherbal formulation by comparing the colour change after chemical analysis, Fluorescence analysis, retention time by thin layer chromatography and functional groups by fourier transform infrared spectroscopy. Conclusions: Flavonoids may responsible for its activity as wound healing. It may work with other bioactive compounds as synergistic effect.

}, keywords = {Clinacanthus nutans, Elephantopus scaber, Flavonoids, Herb-Herb combination, Polyherbal formulation, Wound healing.}, doi = {10.5530/pj.2016.6.4}, author = {Muhammad Shahzad Aslam and Muhammad Syarhabil Ahmad and Awang Soh Mamat} } @article {72, title = {Component Authentication and Standardisation of an Anti-atherosclerotic Herbal Formulation-GSTC3}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Nov-Dec 2015}, pages = {339-343}, type = {Original Article}, chapter = {339}, abstract = {

Context: Polyherbal drugs in traditional medicine have been time-tested for treatment of a wide variety of chronic ailments. Aims: To determine constituent components in an in-house designed polyherbal formulation GSTC3 and evaluation of consistency in preparation. Settings and Design: In this study, a hypolipidemic formulation was created using well known plant extracts such as Commiphora mukul (Hook. ex Stocks.) Eng. (Burseraceae), Salacia reticulata Wight (Celastraceae), Terminalia arjuna (Roxb.) Wight and Arn (Combretaceae) and Curcuma longa Linn (Zingiberaceae). Methods and Material: The authentication of individual plant powders was performed using techniques such as powder microscopy and characterised according to Ayurvedic pharmacopeia of India. Phytochemical screening, HPTLC analysis and physicochemical parameters were also determined. Results: The powder microscopic analysis of the individual components served as an authentication for source of plants used. Phytochemical screening ascertained that active classes of compounds reported in the individual extracts such as steroids, terpenoids and polyphenols came into the formulation. Finally, HPTLC analysis of three different batches of GSTC3 ensured stability and integrity in batch to batch preparations. Conclusion: Experimental studies have revealed the antioxidant, antiinflammatory, anti-lipidemic and anti-thrombotic efficacy of GSTC3. This standardisation procedure is essential for further development of GSTC3 into an efficient anti-atherosclerotic drug candidate

}, keywords = {HPTLC, Pharmacognosy, Phytochemical screening, Polyherbal formulation, Powder microscopy}, doi = {10.5530/pj.2015.6.4}, author = {Jeksy Jos Manalil and Indu Muraleedharan Suseela and Smitha Koyickalmadhom Ramavarma and Arunaksharan Narayanankutty and Achuthan Chathrattil Raghavamenon} } @article {1489, title = {Development and validation of a RP-HPLC method for the simultaneous determination of Mangiferin, Ellagic acid and Hydroxycitric acid in polyherbal formulation}, journal = {Pharmacognosy journal}, volume = {6}, year = {2014}, month = {8th April 2014}, pages = {23-28}, type = {Original Article}, abstract = {

The US patented polyherbal formulation for the prevention and management of Type II diabetes and its vascular complications was used for the present study. The formulation consists of roots of Salacia species, leaves of Lagestroemia parviflora and fruit rind of Garcinia indica. The use of reversed phase C18 HPLC column was used and eluted with isocratic mobile phase of acetonitrile and phosphoric acid buffer solution enabled the efficient separation of chemical markers within 20min. Validation of the method was performed in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery. All calibration curve shows good linear correlation coefficients (r2\>0.995) within tested ranges. Three markers in this polyherbal formulation were quantified were Mangiferin (1.53\% w/w), Ellagic acid (0.9655 w/w), Hydroxycitric acid (5.3\% w/w). Intra and inter day RSDs of retention times and peak areas were less than 3\%. The recoveries were between 95\% and 102.5\%. In conclusion a method has been developed for the simultaneous quantification of three markers in this polyherbal formulation. The established RP-HPLC method was simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.

Key words: Polyherbal formulation, Mangiferin, Ellagic acid, Hydroxycitric acid, RP-HPLC.

}, keywords = {Ellagic acid, Hydroxycitric acid, Mangiferin, Polyherbal formulation, RP-HPLC}, author = {Ananth Kumar Kammalla, and Mohan Kumar Ramasamy, and Agarwal Aruna, and Dubey GP, and Ilango Kaliappan} }