@article {981, title = {Qualitative and Quantitative Determination of Organic Acids in Crude Herbal Drugs and Medicinal Herbal Preparations for Quality Control in Russian Federation by Modern Physicochemical Methods}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {September 2019}, pages = {1132-1137}, type = {Research Article}, chapter = {1132}, abstract = {

Background: Organic acids (malic, citric, tartaric, oxalic, acetic, formic, isovaleric, ascorbic acids) make up a large group of biologically active substances and play an important role in plant and human metabolism. They are found in large quantities in the fruits of Rosaceae family medicinal plants that included in State Pharmacopoeia of the Russian Federation. Standardization of crude herbal drugs containing organic acids by modern physicochemical methods is a high-priority task. Materials and Methods: The determination of total organic acids amount was carried out in aqueous extracts from different fruits of Rosaceae family plants by galvanostatic coulometry and potentiometry methods. Galvanostatic coulometry was performed with the help of the {\textquotedblleft}Expert-006{\textquotedblright} coulometer with a current of 5 mA (integrated pH meter). Iodine as an electrogenerated titrant was used for ascorbic acid determination; electro generation of hydroxide ions was carried out for determination of total organic acids amount. A potentiometer {\textquotedblleft}Aquilon pH-410{\textquotedblright} with attached glass and silver chloride electrodes was used for potentiometric determination of total organic acids amount. Individual organic acids have been determined by reverse-phase high-performance liquid chromatography with ultra-violet detection (RP-HPLCUV) method. The following conditions were established: Gilson HPLC system, Alltech OA- 1000 Organic Acids (6.5{\texttimes}300 mm, 9 μm) chromatography column, a gradient elution mode, component A of the mobile phase is 98\% (0.1\% phosphoric acid, 10 mM KH2PO4, solution in water) with 2\% acetonitrile, component B is acetonitrile, the eluent feed rate is 1 ml/min. Results: Modern physicochemical methods for the analysis of biologically active substances, organic acids, for quality control of crude herbal drugs and medicinal herbal preparations, are developed and discussed. The optimal conditions for the qualitative and quantitative organic acid analysis are selected and described taking into account modern pharmacopoeial requirements. Conclusion: Galvanostatic coulometry and potentiometry methods, as well as RP-HPLC-UV, can be successfully used in the quality control of crude herbal drugs and medicinal herbal preparations, specifically fruits of Rosaceae family plants. Development and validation of analytical methods for monitoring the content of this BAS group is an important research area in the pharmacopoeial standardization of crude herbal drugs.

}, keywords = {Coulometry, Crude herbal drugs, High Performance Liquid Chromatography, Organic acids, Potentiometry, Redox titration, Titrimetric Methods}, doi = {10.5530/pj.2019.11.176}, author = {Ekaterina Vyacheslavovna Sergunova and Alla Anatolyevna Sorokina and Dmitry Olegovich Bokov and Anna Igorevna Marakhova} } @article {755, title = {Comparison between High Performance Thin Layer Chromatography and High Performance Liquid Chromatography Methods for Determination of Rubraxanthone in the Stem Bark Extract of Garcinia cowa Roxb}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {November 2018}, pages = {s42-s47}, type = {Original Article}, chapter = {s42}, abstract = {

Objectives: To develop simple, rapid, accurate methods for determination of rubraxanthone in the stem bark extract of Garcinia cowa using High Performance Thin Layer Chromatography (HPTLC) and High Performance Liquid Chromatography (HPLC). Methods: The HPTLC method was performed on aluminum plate precoated with silica gel 60 F254 using Chloroform: Ethyl acetate: Methanol: Formic acid (88:2:2:8) as a developing system. Quantification was achieved using densitometric measurements at 243 nm. The HPLC method involved a 5 \μm C18 column and an isocratic solvent using 0.4\% formic acid: methanol (12:88) with a flow rate 1 mL minute-1. Quantitation was also achieved with ultraviolet detection at 243 nm based on peak area. All necessary validation tests for both methods were done for their comparison. The results obtained by these two different quantification methods were compared by Tukey\’s-test. Results: Both assays provided good linearity, accuracy, precision, specificity and limits of detection and quantitation for determination of rubraxanthone in The Stem Bark extract of G. cowa. Conclusion: Both methods revealed reasonable validation parameters concerning linearity, accuracy, precision, specificity and limits of detection and quantitation. A statistical comparison of the quantitative analysis of rubraxanthone in extract did not show any statistically significant difference between two analysis methods. As both methods were found to be equal, they therefore can be used for the analysis of rubraxanthone in the Stem Bark extract of G. cowa.

}, keywords = {Garcinia cowa Roxb, High Performance Liquid Chromatography, High performance Thin layer Chromatography, rubraxanthone}, doi = {10.5530/pj.2018.6s.8}, author = {Meri Susanti and Sanusi Ibrahim and Yahdiana Harahap and Dachriyanus} }