02227nas a2200241 4500008004100000245014900041210006900190260001200259300001000271490000600281520144500287653002101732653001701753653001801770653001301788653001301801100003301814700002101847700003201868700003001900700002201930856003301952 2015 eng d00aEvaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil0 aEvaluation of cytotoxic DNA protecting and LPS induced MMP9 down c01/2015 a32-360 v73 a

Introduction: Plectranthus amboinicus (Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthusamboinicusto test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53 ± 0.01 and 87 ± 0.01 μg/mL respectively. At 200 μg/mL essential oil protected against 75% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations.

10aAntigenotoxicity10aCytotoxicity10aEssential oil10aRT- PCR.10aZymogram1 aThirugnanasampandan, Ramaraj1 aRamya, Gunasekar1 aGogulramnath, Madhusudhanan1 aJayakumar, Rajarajeswaran1 aKanthimathi, M.S. uhttp://phcogj.com/article/32