Adulteration of Herbal Medicine and its Detection Methods - Arwa Ahmed Alyas; Hasan Aldewachi; Mohammed Ibrahim Aladul (Pharmacognosy Journal, 2024)
People are increasingly turning to the use of herbal medicines (HMs) due to the growing trend of embracing nature and concerns about the adverse effects of conventional treatments. HMs provide a sense of safety because they are natural and intended for long-term use. However, herbal medicines are also associated with adverse effects. Furthermore, the use of these medicines poses dangers associated with the deliberate inclusion of synthetic substances, the intentional or accidental substitution of plant species, or simply the risk of mislabeling. While reports of illegal synthetic or pharmaceutical substances being added often involve herbal weight-loss and weight gain preparations, aphrodisiacs, treatments for rheumatic and inflammatory diseases, antidiabetic medications, and antihypertensive preparations, it appears that the substitution of plant species occurs across various categories of herbal medicines. This narrative aims to review the types of adulteration in herbal medicine, the analytical techniques used for detecting adulteration. In conclusion, more studies on the adulteration of HMs are required to inform health authorities and limit the use of these substances. Therefore, it is necessary to coordinate and encourage regulatory policies on HMs on a worldwide scale. Relevant regulatory agencies all over the world must be proactive and keep enforcing the necessary safeguards to protect public health by ensuring that all herbal medications approved for sale are secure and of a high enough standard.
Keywords: Adulteration; detection methods; Herbal medicines

Analysis of Factors Influencing the Willingness to Pay BPJS Kesehatan Contributions of Mandiri Participants in the Working Area of Bua Health Center, Luwu District - Syiar Cakke. S; Balqis; Indar; Sukri Palutturi; Hasanuddin Ishak; Andi Zulkifli; Anwar Mallongi (Pharmacognosy Journal, 2024)
The willingness to pay BPJS Health contributions regularly and on time every month for independent participants is the most important component to make it easier for independent participants to utilize health services. This research aims to analysis the factors that influence the willingness to pay BPJS Health contributions for independent participants in the Bua Health Center working area, Luwu Regency. The research was conducted in the working area of the Bua Health Center, Luwu Regency. The research design iscross sectional study. The total sample was 340 independent BPJS Health participants using sampling techniquesSimple Random Sampling. Data were analyzed using testsChi-Squareand Multiple Logistic Regression test. Test resultsChi-Squareshows that there is a relationship between the number of family members (p=0,000), income (p=0,000), perception of the quality of health services (p=0,000), history of catastrophic illness (p=0,000), and ability to pay BPJS Health contributions (p= 0,000) with a willingness to pay BPJS Health contributions. Meanwhile, there is no relationship between knowledge (p=0,364) and willingness to pay BPJS Health contributions. The results of the Multiple Logistic Regression test show that number of family members (OR=69,893) is the factor that most influences the willingness to pay BPJS Health contributions. It is recommended that the BPJS Health consider a contribution payment system for independent participants who are required to pay contributions collectively which includes all family members in one family who are registered as independent BPJS Health participants, so that independent participants who have large family members are not burdened and are willing to pay BPJS contributions Health regularly and on time every month.
Keywords: BPJS Health; Income; Independent Participants; Number of Family Members; Willingness to Pay Contributions

Analysis of Hepcidin and Interleukin-6 Levels among Transfusion-Dependent Thalassemia Patients With and Without Alloimmunization/Autoimmunization - Widya Firly Novitasari; Jusak Nugraha; Mia Ratwita Andarsini; Betty Agustina Tambunan (Pharmacognosy Journal, 2024)
Background: Transfusion-dependent thalassemia (TDT) necessitates regular transfusions, resulting in complications such as iron overload, hemolytic anemia, and the emergence of alloantibodies/ autoantibodies. This situation poses challenges in obtaining compatible transfusions. Excessive iron and chronic hemolysis impact the elevation of Interleukin-6 (IL-6), initiating an inflammatory process that triggers hepcidin formation and influences antibody development. This study aims to analyze disparities in IL-6 and hepcidin levels and establish the correlation between IL-6 and hepcidin in TDT patients with and without alloimmunization/autoimmunization. Methods: Forty whole blood samples were collected from TDT patients with and without alloimmunization/autoimmunization, centrifuged, and the serum extracted, then stored in a refrigerator at -80°C. IL-6 and hepcidin levels were assessed using the ELISA method. The Mann-Whitney U test was employed to evaluate differences in hepcidin and IL-6 levels between the two groups. In contrast, the Spearman Correlation test was utilized to analyze the correlation between hepcidin and IL-6 levels. Results: IL-6 levels in the TDT group with alloimmunization/autoimmunization (3.64 pg/ mL) were significantly higher compared to the TDT group without alloimmunization/autoimmunization (1.41 pg/mL; p < 0.05). Hepcidin levels in the TDT group with alloimmunization/autoimmunization (2,950.6 pg/mL) were significantly higher compared to the TDT group without alloimmunization/autoimmunization (1,599.6 pg/mL; p < 0.05). The Spearman correlation test revealed a significant positive correlation between hepcidin and IL-6 levels in TDT patients with alloimmunization/autoimmunization (r = 0.764; p = 0.000). Additionally, a significant positive correlation was observed between hepcidin and IL-6 levels in TDT patients without alloimmunization/autoimmunization (r = 0.559; p = 0.010). Conclusion: IL-6 and hepcidin levels were elevated in TDT patients with alloimmunization/autoimmunization compared to those without. Interleukin-6 and hepcidin exhibited a positive correlation in both transfusion-dependent thalassemia groups.
Keywords: Anemia; Antibodies; Hepcidin; Interleukin-6; Transfusion-Dependent Thalassemia

Androgenic Efficacy and Mechanism of Glycosides-Based Standardized Fenugreek Seeds Extract Through Aromatase And 5-Alpha Reductase Inhibition - Urmila M Aswar; Savita R. Nimse; Prasad A. Thakurdesai (Pharmacognosy Journal, 2024)
Introduction: Fenugreek seeds glycosides content have many health benefits. Objective: To evaluate the androgenic efficacy and probable mechanism of glycosides-based standardized fenugreek seed extract (SFSE-G) in laboratory rats. Methods: Male Wistar rats were administrated with 28-days of once-daily oral administration of SFSE-G (10 or 35 mg/kg) on sexual and orientational behavior with female rats, serum testosterone concentrations, weights of reproductive system-related organs (seminal vesicles, prostate, levator ani), nitric oxide level in penis homogenate, sperm count in the cauda epididymis, and testis histology were evaluated. Separate groups of rats with a positive control (testosterone propionate (10 mg/ kg, s.c. bi-weekly) and vehicle control (distilled water) were maintained. In addition, the safety of acute intravenous administration of SFSE-G (1 mg/kg) on cardiovascular function parameters was evaluated. Moreover, the inhibitory potential of SFSE-G against aromatase and 5-alpha-reductase enzymes was evaluated in vitro. Results: Subacute administration of SFSE-G (35 mg/kg, oral) to male rats showed androgenic efficacy in sexual behavior (increased mounting and intromission latency and rearing), with increased weights of seminal vehicles, prostate and levator ani muscles, serum testosterone levels, sperm count, and penile NO concentration, while preserving the normal architecture of the testes. Acute intravenous administration of SFSE-G to rats increased intracavernous pressure but retained normal cardiovascular parameters, such as blood pressure, heart rate, and corrected QT interval (QTc). SFSE-G showed significant inhibition of aromatase and 5-alpha-reductase in vitro. Conclusion: SFFE-G exhibited significant androgenic and spermatogenic efficacy, mediated through testosterone metabolism inhibition, without affecting the cardiovascular system in laboratory rats.
Keywords: Androgenic; Fenugreek extract; Glycosides; Sexual Behavior; Spermatogenic; Testosterone

Antibacterial Potential of Durian (Durio zibethinus murr.) Waste as an Alternative Disinfectant in Raw Drinking Water - A Literature Review - Rahwan Ahmad; Ridwan Amiruddin; A.Arsunan Arsin; Stang Stang; Hasanuddin Ishak; Yahya Thamrin; Anwar Mallongi (Pharmacognosy Journal, 2024)
Efforts continue to be made to eliminate pathogenic bacteria in drinking water by using safer disinfection materials. The use of local plants as disinfection materials is highly desirable. The durian plant (Durio zibethinus) can be a very promising alternative as a natural disinfection agent to destroy pathogenic bacteria in drinking water. This narrative review comes from three online journal databases that provide free journal articles in PDF format, collected over the last ten years. The main keywords in conducting a literature review through online journals are "Durian, Durio Zibethinus, antibacterial, disinfectant, drinking water". Of the 5,693 journals identified in the three databases, 21 studies remained that met the requirements for inclusion in this review. This included study illustrates that fruit rind, seed, and leaf extracts from the durian plant have antimicrobial properties. Durian plants have antibacterial active compounds such as phenolics, alkaloids, steroids, saponins, tannins, flavonoids and triterpenoids. The antimicrobial potential tested using the diffusion and dilution method indicated that the presence of antimicrobial ingredients in the durian plant extraction was in the strong category. Some pathogenic bacteria in water that can be inhibited or killed are Escherichia coli, Pseudomonas aerugenosa, Bacillus subtilis, Bacillus cereus, Enterococcus faecalis, Staphilococcus aureus, Shigella boydii and Salmonella Typhi. This shows that there is an opportunity for the durian plant to provide a solution and great hope as a safe and economical alternative disinfection material as a replacement for current disinfection materials. Index terms: Durian (Durio zibethinus), antibacterial, disinfectant, drinking water.

Bacteriological Profile of Clinical Isolates from COVID-19 Hospitalised and Non-Hospitalised Patients in Bloemfontein - Matsaba B.M; Makhoahle P.M (Pharmacognosy Journal, 2024)
The COVID-19 literature is limited regarding bacterial infections. If bacterial infections drive mortality in respiratory viruses, this has clear implications for patient management. To address this limited literature problem, we review current knowledge on bacterial infections in COVID-19, assess information from past viral respiratory pandemics, and determine the most prevalent infections. We also study antibiotic misuse during the COVID-19 pandemic. The findings of this study conclude that there is a need to consider presence or absence of bacterial infection in COVID-19 management. Early determination and antibiotic treatment are of importance, however not all patients who are tested for COVID -19 are also tested for bacterial infections which may exacerbate the disease. Physicians utilised antibiotics as a treatment for COVID -19 for various reasons, this posed a risk of antibiotic overuse and antimicrobial resistance when administered in patients with no bacterial infection. While lack of access to antibiotics could be dangerous in the same vein as its misuse, it is of importance to ensure that these life-saving agents are preserved and used with utmost care.
Keywords: Antibiotics; Bacterial Infections; COVID-19; Patients

Bioguided Assay of Polyphenols Isolated from Medicinal Mayan Species and its Activity Against Leishmania mexicana. - Horacio Larqué; Abelardo Chávez Montes; Jaime Zamora-Chimal; Moises Looh-Hernández; Joel H. Elizondo Luevano; Esther del Olmo (Pharmacognosy Journal, 2024)
Objective: This study underlines the in vitro leishmanicidal activity of the methanol extracts (MeOH), fractions of n-hexane (n-Hex), chloroform (TCM) and ethyl acetate (EtOAc), and compounds isolated from plant species used in the Mayan traditional medicine. Materials and Methods: Extracts of medicinal species collected in the Mayan Peninsula such as Hylocerus undatus, Bauhinia divaricate, Euphorbia hirta, Ruellia nudiflora and Cedrela odorata, were tasted in a bio guided assays against amastigotes of Leishmania mexicana. Different chromatographic techniques were applied in order to isolated the most active compounds. Additionally, spectroscopic experiments 1H-NMR, 13C-NMR, LC-MS and FT-IR were stablished to determine the chemical structure of the chemical compounds. Results: Euphorbia hirta and Cedrella odorata, showed good bioactivity with 14.81 ± 2.63 g/mL and IC50 = 18.39 ± 0.88 μg/mL respectively, meanwhile Bauhinia divaricata not show activity and Ruellia nudiflora showed poor activity with IC50 = 92.18 ± 3.64 μg/mL, followed by Hylocerus undatus with IC50 = 122.5 ± 20.99 μg/mL, when tasted against amastigotes of Leishmania mexicana. Spectroscopic data confirmed the presence of quercetin, myricetin, kempherol and scopoletin, with IC50 = 2.92 ± 0.42 μM, 12.30 ± 0.57 μM, 20.22 ± 4.66 μM and 4.05 ± 0.68 μM respectively. Conclusion: The bioguided assays guided us, to the purification and isolation of four different metabolites, mainly flavonoids and structurally related compounds, some of them show good activity, however, their low bioavailability indicates the need for detailed structural relation activity studies, together with the development of formulations and delivery systems.
Keywords: Coumarins; Flavonoids; Leishmania mexicana; NMR structural determination; Polyphenols

Chamomile (Matricaria chamomilla L.) Essential Oil and its Potential Against Stress, Anxiety, and Sleep Quality - Paul Alan Arkin Alvarado-García; Marilú Roxana Soto-Vásquez; Elda Maritza Rodrigo-Villanueva; José Gilberto Gavidia-Valencia; Natalia Mavila Guzmán Rodríguez; Roger Antonio Rengifo-Penadillos; Julio Víctor Campos-Florián; Yolanda Elizabeth Rodríguez de Guzmán (Pharmacognosy Journal, 2024)
Introduction: This investigation aimed to evaluate the effect of Matricaria chamomilla L essential oil on stress, anxiety, and sleep quality. Methods: The essential oils were extracted by hydrodistillation using a modified Clevenger-type apparatus, and the chemical composition was performed by Gas chromatographymass spectrometry (GC–MS). Furthermore, a study was conducted where 128 participants were divided into two groups: a control group and an experimental group treated with essential oils. Stress, anxiety, and sleep quality were evaluated using the Perceived Stress Scale (PSS–10), the Zung Self-Rating Anxiety Scale (SAS), and the Pittsburgh Sleep Quality Inventory (PSQI). In addition, cortisol was measured. Results: Chemical analysis showed thatα-Bisabolol oxide A exhibited the highest concentration at 30.42%, followed by α -Bisabolol (10.94%), α-Bisabolone oxide A (9.82%), and spathulenol (8.83%). Stress, anxiety, sleep quality, and cortisol scores decreased in the post-test study phase compared to the pre-test in the experimental group (p<0.05). Important effect size was found in the case of anxiety (d = 1.203; Δ = 1.246), while medium effects were observed for stress (d = 0.701; Δ = 0.633) and sleep quality (d = 0.888; Δ = 0.732), while cortisol showed small changes (d=0.374; Δ=0.357). Conclusion: The study showed the potential of Chamomile essential oils to reduce stress and anxiety levels and improve sleep quality.
Keywords: Anxiety; Essential oils; Matricaria chamomilla; Sleep quality; stress

Community-based Anti-Drug Efforts: Leveraging Local Wisdom for Prevention - T Alamsyah; Teuku Muliadi; Khairunnas; Sufyan Anwar; Marniati; Anwar Mallongi (Pharmacognosy Journal, 2024)
Background: Drug abuse prevention in the community based on local culture, specifically “Pageu Gampong,” involves legal aspects related to drugs and the management of drug prevention in the community. This study examines the influence of local culture, “Pageu Gampong,” as a preventive measure against drug use among ninety in Aceh Besar district. Materials and Methods: In this study, a quasi-experimental pre-post test design was utilized to investigate the effectiveness of drug abuse prevention training that incorporated local culture, specifically, “Pageu Gampong”; the research was conducted in seven phases and yielded promising results, including the establishment of an anti-drug task force team aimed at sustaining drug control efforts at the village level. To ensure data accuracy, the researcher designed and tested a self-administered questionnaire for validity and reliability. Results: The research showed increased general knowledge about drugs, legal aspects related to drugs, and drug prevention procedures with a p-value of 0.000. The results of this study are expected to be used for screening and early detection of the risk of drug abuse in the community through various culturally-based programs implemented by task force team members. Conclusion: This study can be used for screening and early detection of the risk of drug abuse in the community through various culturally-based programs implemented by task force team members.
Keywords: Anti-drug task force; Community-based programs; Drug prevention; Local culture; Risk detection

A Comparative Study of the Biometric, Germinative, and Physicochemical Characteristics of Fruits and Oils of Three Cannabis Strains (Cannabis sativa L.var indica) Cultivated in the Rif Region of Morocco - Abdelhak Chergui; El Amine Ajal; Imane Zakaria; Rachid Nejjari (Pharmacognosy Journal, 2024)
Background: Cannabis sativa var indica is the variety grown in the northern reliefs of Morocco, exclusively for resin and narcotics production. Despite the extensive research on the botany, phytochemistry, and pharmacology of Rif's cannabis, there is limited comparative research on the fruits and oils of different strains cultivated in the region. Objective: This study examined the biometric, germinative and physicochemical properties of three cannabis strains grown in the Western Rif region of Morocco. Materials and methods: three cannabis strains (Beldiya, Khardala and Critical) were characterized by their biometric parameters (weight, length and width), germinative and physicochemical properties. Results: The study revelated that there are notable differences in the biometric properties and germination rates of the achenes of the strains studied. Additionally, significant variations were observed between the strains with regards to their oil yield, as well as the levels of polyphenols and flavonoids. The results also revealed a correlation between biometric parameters, germination rate and phytochemical content. Conclusion: The oils of all strains studied were extra virgin, and the Beldiya strain's fruit oil was found to be particularly rich in polyphenols and flavonoids, making it a potentially useful ingredient in nutrition and health applications. Overall, this study provides useful information for researchers and growers interested in the cultivation and use of cannabis fruits and oils in various industries.
Keywords: Biometry; Cannabis strains; Fruit; germination; Oil; Rif

Concordance of Sputum and Feces Samples for Detecting Mycobacterium Tuberculosis using Xpert® MTB/RIF Ultra - Dewi Rochmawati; Puspa Wardhani; Yessy Puspitasari; Tutik Kusmiati; Atika; Hartono Kahar (Pharmacognosy Journal, 2024)
Introduction: Tuberculosis (TB) remains a disease with high morbidity and mortality worldwide, and Indonesia ranks among the countries with the highest TB prevalence. There is a need to develop improved detection tools and explore alternative sample sources beyond sputum. Feces samples are one such nonsputum alternative. Xpert® MTB/RIF Ultra is a novel diagnostic tool used in Indonesia. This study aims to assess the concordance of both sputum and feces samples in detecting Mycobacterium tuberculosis (MTB) using Xpert® MTB/RIF Ultra. Methods: An analytical observational study with a cross-sectional design was conducted on TB subjects at Dr. Soetomo Regional Public Hospital (RSUD Dr. Soetomo), Surabaya, and several community health centers (puskesmas) in Surabaya. Sputum and feces samples were collected from the same subjects. These subjects underwent Feces acid-fast bacilli (AFB) tests, sputum and Feces Xpert® MTB/RIF Ultra tests, and sputum culture tests (considered the gold standard), as well as rifampicin resistance tests for positive cultures. Sensitivity and positive predictive value (PPV) tests were conducted using Medcalc software, and the concordance test employed the Kappa value. Results: The study involved 71 research subjects. The sensitivity of Feces AFB tests, sputum, and Feces Xpert® MTB/RIF Ultra tests was 7.3%, 97.6%, and 97.6%, respectively. The Cohen's Kappa consistency test for Feces AFB tests and sputum culture produced a Kappa value of 0.063 (p > 0.05). The Cohen's Kappa consistency test on sputum and Feces Xpert® MTB/RIF Ultra tests yielded a Kappa value of 0.409 (p < 0.05). The Cohen's Kappa consistency test on sputum and Feces Xpert® MTB/RIF Ultra tests compared with the rifampicin resistance tests resulted in Kappa values of 0.902 and 0.951 (p < 0.05). The CT value of Feces Xpert® MTB/RIF Ultra tests was higher than that of sputum Xpert® MTB/RIF Ultra tests. Conclusion: A concordance exists between the results of sputum and Feces Xpert® MTB/RIF Ultra tests, but no concordance is observed between the results of Feces AFB tests and sputum culture tests. The higher CT value of Feces Xpert® MTB/RIF Ultra tests compared to sputum Xpert® MTB/RIF Ultra tests indicates a lower bacterial load in feces. Feces can be considered a viable alternative sample to sputum for MTB detection using Xpert® MTB/RIF Ultra.
Keywords: Feces AFB test; Feces Xpert® MTB/RIF Ultra test; Mycobacterium tuberculosis (MTB); rifampicin resistance test; Xpert® MTB/RIF Ultra

The Effect of Glycopyrrolate to Support Fentanyl on Reducing Airway Irritation during Inhalation Induction with Desflurane and Nitrous Oxide in Adult Patients - Erwin Mulyawan; Clarissa Jasmine Aurelia (Pharmacognosy Journal, 2024)
Introduction: Inhalational induction of anesthesia is occasionally used in adults. Desflurane is not used for the induction of anesthesia despite its favorable pharmacokinetic characteristics as it causes airway irritation. This study aims to identify a reduction of airway irritation with pretreatment using fentanyl and glycopyrrolate. Methods: A total of 80 adults were randomized into two groups to receive fentanyl and glycopyrrolate (FG group) or fentanyl only (FS group) prior to desflurane administration. Time between desflurane administration and loss of consciousness was recorded as well as expired desflurane concentration. Signs of airway irritation including coughing, apnea, laryngospasm, and excitatory movements at time of induction was also recorded. Unpaired t test, Fisher’s Exact test, and Chi-square test were used to analyze parametric data and all non-parametric data was analyzed using the Mann– Whitney test. Results: This study found time to loss of consciousness was 4 minutes. Incidence of airway irritation in Group FS vs FG including cough, apnea, excitatory movements, and laryngospasm was (20% vs 2.5%), (0% vs 0%), (5% vs 5%), and (7.5% vs 0%) respectively. Total airway irritation was 32.5% in FS compared to 7.5% in FG, with P value 0.0103. Conclusion: Use of fentanyl and glycopyrrolate decreases airway irritation incidence caused by desflurane in comparison to use of fentanyl alone. This opens the possibility to commonly use desflurane as inhalation induction of anesthesia as its favorable pharmacokinetics may be utilized whilst limiting the adverse effects it causes.
Keywords: Airway Irritation; Desflurane; Fentanyl; Glycopyrrolate

Effect of Lactiplantibacillus plantarum IS-10506 on Accelerating Repair of Ketorolac-Induced Gastric Ulcers in Wistar Rats - Mia Ratwita Andarsini; Andy Darma; Alpha Fardah Athiyyah; Khadijah Rizky Sumitro; Reza Gunadi Ranuh; Fauziah Pratiwi; Wibi Riawan; Widjiati Widjiati; Ingrid S Surono; Subijanto Marto Sudarmo (Pharmacognosy Journal, 2024)
A gastric ulcer arises due to an imbalance between the stomach's aggressive and defensive factors, one of which can be induced by ketorolac. The gastric mucosa serves as a protective layer against gastric damage. Probiotics may enhance mucosal secretion, reinforcing their barrier function. This study evaluated the effect of Lactiplantibacillus plantarum IS-10506 on repairing gastric mucosal injury expressed by Mucin 5AC (MUC5AC) induction from ketorolac in rodent model. In the experiment, 48 male Wistar rats were randomly assigned and segregated into four groups: the control group (K1), the group with ketorolac administration (K2), the group given L. plantarum IS-10506 after ketorolac administration (K3), and the group given L. plantarum IS-10506 before and after ketorolac administration (K4). Gastric tissue was examined for cells producing MUC5AC via immunohistochemistry. MUC5AC differences between groups were compared using Kruskal–Wallis and Mann–Whitney U tests. Significant differences were observed between each group on Days 5, 7, and 10 of necropsy (p=0.043; p=0.030; p=0.022). The ketorolac group (K2) consistently exhibited the lowest values during all examination days. Group K4 manifested a higher expression of MUC5AC relative to group K3. However, group K3 demonstrated a significantly increased from Day 1 to 10 of necropsy (p=0.030). Administering probiotic L. plantarum IS-10506 prior to ketorolac proved beneficial by significantly accelerate (p=0.030) the MUC5AC expression cells and gene expression.

Effect of Plasma Treatment on Tensile Bond Strength of (5) Yttrium Zirconia Coping Fixed on Titanium Implant Abutment - Amani Abduljabar Altaie; Emad Farhan Alkhalidi (Pharmacognosy Journal, 2024)
Objective: The goal of this research is to investigate the effect of plasma surface treatment on retentive strength (RS) between the (5)Yttrium Zirconia coping and the titanium implant abutment (Dentium system) using self-adhesive resin cement. Methods: forty standard titanium implant abutments of 5.5 mm height and 4.5 mm in diameter were screwed with implant laboratory analogs embedded vertically in autopolymerizing acrylic resin blocks, forty Zirconia copings with a hole on the occlusal surface were fabricated using CAD/CAM system. Samples were divided into four groups (n 10 for each group); Group (1) no plasma surface treatment for implant abutment and coping (act as control). Group (2) plasma surface treatment for implant abutment only. Group (3) plasma surface treatment for coping only. Group (4)plasma surface treatment for implant abutment and coping. Coping of all groups were cemented with implant abutment by Allcem dual cure resin cement. All cemented samples were (5000) cycles of thermocycling process and then tensile bond strength evaluated by Universal testing machine, Following the tensile bond strength test, each debonded surface was examined by stereomicroscope at a 20x magnification. The one-way analysis of variance and Duncan test were used to statistically examine the experimental results. Results: Plasma surface treatment had observed effect that increase tensile bond strength between titanium implant abutment and (5) yttrium Zirconia coping, plasma surface treatment for abutment and coping (Group 4) was the highest value of tensile strength followed by abutment plasma surface treatment (Group 2), while lowest value of tensile bond strength in control group (Group 1) and coping plasma surface treatment (Group 3). Conclusion: Plasma surface treatment increase tensile bond strength between implant abutment and zirconia coping. Plasma surface treatment for both abutment and coping provided higher tensile bond strength. Mode of failure was adhesive failure occurs in abutment surface.
Keywords: (5)Y Zirconia. Tensile bond strength; Plasma; Titanium abutment

Effects of Cerebrospinal Fluid Drainage on Pro-Inflammatory and Anti-Inflammatory Cytokines Expression in the Subventricular Zone of Kaolin-Induced Hydrocephalic Rats - Muhammad Arifin Parenrengi; Ahmad Data Dariansyah; Wihasto Suryaningtyas; Dyah Fauziah; I Ketut Sudiana; Budi Utomo; Prastiya Indra Gunawan (Pharmacognosy Journal, 2024)
Background: To determine the neuroprotective effect of CSF drainage by analyzing its impact on the expression and the ratio of pro- and anti-inflammatory cytokines in the subventricular zone in kaolininduced hydrocephalic rats. Method: Sprague-Dawley rats of 23 weeks of age (n=36) were used in this study. The rats were randomly divided into normal control, hydrocephalus, and CSF drainage-treated groups. Hydrocephalus was obtained by injecting 0,05 cc of 20% kaolin suspension into the cisterna magna. The CSF drainage-treated group had ventricular tapping seven days after kaolin induction. The rats were sacrificed 7, 14, or 21 days after kaolin induction. The brain was removed and prepared for immunohistochemistry analysis to detect IL-1β, IL-6, TNF-α, and IL-10 cytokines expression. Results: Immunohistochemistry analysis revealed that the expression of pro-inflammatory cytokines was significantly increased in hydrocephalus groups than in the control group. In contrast, the expression of anti-inflammatory cytokine was significantly decreased. CSF drainage had a neuroprotective effect by reducing pro-inflammatory cytokine expression and increasing anti-inflammatory cytokine expression. In the hydrocephalus group, the ratios of IL-1β/IL-10, IL-6/IL-10, and TNF-α/IL-10 increased toward a pro-inflammatory status. After CSF drainage, the ratios of IL-1β/IL-10, IL-6/IL-10, and TNF-α/IL-10 shifted toward an anti-inflammatory status. Conclusion: CSF drainage protects the brain from excessive neuroinflammatory processes in kaolin-induced hydrocephalic rats. Additional investigation is warranted to ascertain the use of inflammatory cytokines expression as a valuable biomarker for hydrocephalus. Furthermore, research on anti-inflammatory drug administration in clinical settings is required.
Keywords: cerebrospinal fluid drainage; Cytokines; Kaolin-induced hydrocephalus; Neuroinflammation; Neuroprotective; subventricular zone

Ethnomedicine In Nias Island - Nilsya Febrika Zebua; Nerdy Nerdy; Kanne Dachi; Muflihah Fujiko; Abdi Wira Septama (Pharmacognosy Journal, 2024)
Background: Medicinal plants have an important role in the traditional medicine system of the Nias people, which has been passed down from generation to generation. Although there has been some ethnobotanical research in Indonesia, especially in Nias, studies regarding people's knowledge, use and perception of medicinal plants on this island are still very limited. Objective: to investigate the knowledge, use and perceptions of the Nias community towards medicinal plants. Design: The research population involved communities in various areas of Nias, and samples were taken randomly from several villages or communities. Data collection methods include questionnaires, interviews, and observations to detail the use of medicinal plants, local knowledge, and factors that influence the choice and use of medicinal plants. The research variables involve the demographic characteristics of respondents, education level, access to health information as independent variables, while the dependent variables include the use of medicinal plants and knowledge about medicinal plants. Results: As a result of data collection on respondents on Nias Island, there were 50 medicinal plants that people have often used for generations to treat diseases. Data analysis involved analyzing factors related to the use of medicinal plants. It is hoped that this research will provide a better understanding of the use of medicinal plants in Nias and contribute to the preservation of local knowledge and the development of public health strategies. Conclusion: Most of the empirical data submitted by the local Nias community is in accordance with research that has been carried out, however, there are 13 plants that need to be explored further because the empirical data does not match.
Keywords: Ethnobotanical; Ethnomedicine; Medicinal plant; Nias

Evaluating the Therapeutic Potential of Vernonia amygdalina: A Promising Antidiabetic Agent in STZ and Nicotinamide-Induced Rat Model - Muhammad Faridz Syahrian; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting (Pharmacognosy Journal, 2024)
Background: Vernonia amygdalina, commonly known as bitter leaf, has been traditionally used for its potential antidiabetic properties. This study aimed to evaluate the therapeutic potential of Vernonia amygdalina extract (VAE) in ameliorating hyperglycemia using a streptozotocin (STZ) and high-fat diet (HFD)-induced rat model of diabetes. Methods: Sixty male Wistar rats were divided into six groups: normal control, diabetic control, and four treatment groups receiving different doses of VAE (100, 300, and 500 mg/kg body weight) orally for eight weeks. Diabetes was induced in rats by a single intraperitoneal injection of STZ (55 mg/kg) after four weeks of Nicotinamid feeding. Body weight, fasting blood glucose levels, HbA1c, serum insulin levels, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) levels were measured. Results: Treatment with VAE significantly reduced fasting blood glucose levels in a dose-dependent manner compared to the diabetic control group (p < 0.05). VAE administration also led to a significant decrease in HbA1c levels and an increase in serum insulin levels in a dosedependent manner (p < 0.05). Furthermore, VAE supplementation restored SOD activity and reduced MDA levels, indicating improved antioxidant status in the treated groups (p < 0.05). Conclusion: This study demonstrates the therapeutic potential of Vernonia amygdalina as an antidiabetic agent in the STZ and HFD-induced rat model of diabetes. VAE supplementation effectively reduced fasting blood glucose levels, improved glycemic control as indicated by reduced HbA1c levels, and enhanced insulin secretion. Moreover, VAE exhibited antioxidant activity by restoring SOD activity and reducing MDA levels. These findings suggest that Vernonia amygdalina could be a promising natural remedy for the management of diabetes. Further investigations are warranted to elucidate the underlying mechanisms and evaluate its long-term safety and efficacy in humans.
Keywords: Diabetes; HbA1c; Insulin; MDA; SOD; Vernonia amygdalina

Evaluation of Tumor Necrosis Factor Alpha, Insulin, glucose, HbA1c% and HOMA-IR as Predictors for Cardiovascular Diseases in Patients with Type 2 Diabetes - Haider Nasser Jabber; Bassem Charfeddine; Hamed Jaddoa Abbas (Pharmacognosy Journal, 2024)
Objective: Comparison of the blood level of TNFα in patients with and without type 2 diabetes who have cardiovascular diseases. Examine the connection between the amount of serum TNFα and insulin resistance in patients with type 2 diabetes who have cardiovascular diseases. Method: The present study included 60 patients with Diabetes Mellitus (DM) with a mean age of 56.02±1.395 years and an age range of (40 - 80)years and 60 patients with cardiovascular diseases and diabetes (CVD and DM) with a mean age of 59.20±1.478 and an age range of (40-80) years, Who visited Al-Basrah Teaching Hospital in Basrah. in addition, the study included 60 healthy controls mean age of healthy control subjects was 54.72±1.405years. All patients in this study were diagnosed by specialized doctors and the diagnosis was verified by clinical and laboratory tests, during the period from September 2022 to September 2023. All Subjects signed a written informed consent form. The BMI was calculated as body weight (kg) and was divided by squared height in meters. Results: The results of this study showed an increase in the level of glucose, haemoglobin A1c%, insulin, and HOMA IR (in CVD and DM patients as compared with DM patients and control and there was a significant difference in concentrations among study groups (p-value <0.0001). Also, The results of this study showed an increase in the level of tumor necrosis factor-alpha in CVD and DM patients as compared with DM and control and there was a significant difference in concentrations of TNFα among study groups (p-value <0.0001). Conclusion: Based on the findings of this research, it can be inferred that TNFα and HbA1c have the potential to serve as practical and straightforward indicators for predicting the coexistence of insulin resistance, dysglycemia, and Cardiovascular Diseases in seemingly healthy individuals within the young (<50 years) Al-Basra community.
Keywords: Cardiovascular Disease; Diabetes Mellitus and Inflammation; HOMA-IR; Insulin; TNFα

Impact of Fluoridated Dental Products on Surface Roughness and Morphology of Bleached Tooth Enamel: An In Vitro Study - Mohammad H. Alsabeel; Aisha A. Qasim (Pharmacognosy Journal, 2024)
Purpose: To assess the impact of fluoridated dental products (Toothpaste, mouth rinse, and fluoride varnish) on bleached tooth enamel's surface roughness (SR) and surface morphology when used before, after, or before and after the tooth bleaching process. Methods: A total of (64) bovine permanent incisors were prepared and divided into two main groups: Group (A) was treated before and after the bleaching. Group (B) was treated after bleaching only. The bleaching process used an Opalescence boost of 40% hydrogen peroxide. Each main group was divided into (4) subgroups: (A1, B1) Control, (A2, B2) treated with FluorKIN mouthrinse, (A3, B3) treated with FluorKIN toothpaste, (A4, B4) treated with Proshield FV. A profilometer was used to evaluate surface roughness. Also, to assess the morphological changes in the enamel surface, another (14) teeth samples were viewed under the SEM. Results: There was a statistically significant increase in SR after bleaching and a decrease in SR after treatment, and there was no statistically significant difference between the group receiving bleaching only and the group receiving preventive protocol before bleaching, confirmed by SEM analysis. The slightest increase in surface roughness was found in the fluoride varnish group, followed by the FluorKIN toothpaste group, then the FluorKIN mouthrinse group. Conclusion: According to the surface roughness means results in the present study, Surface roughness increased very little in the fluoride varnish group, followed by the toothpaste group and mouthrinse group, respectively.
Keywords: Bleaching; Fluoride; Morphology; Roughness

Implementation Model of Acceptance of Covid-19 Booster Vaccine in Elderly in Bogor City - Astrid Dewi Prabaningtyas; Ridwan Amiruddin; A Arsunan Arsin; Healthy Hidayanty; Chatarina Umbul W; Ummu Salmah; Lalu Muh Saleh; Sudirman Nasir; Anwar Mallongi (Pharmacognosy Journal, 2024)
Objective: The major impact of COVID-19 occurs especially on the elderly, however the vaccination achievement rate among them tends to be low. The COVID-19 vaccination strategy for the elderly must still be implemented even though WHO has been revoked the Public Health Emergency of International Concern (PHEIC) status for COVID-19. One approach that can be taken is to empower the elderly in the vaccination process. This research aims to see the effectiveness of changes in knowledge, attitudes and behavior of the elderly regarding acceptance of the COVID-19 vaccine after receiving educational intervention using modules using the peer group method. Method: Quasi-experiment with a one-group pretest-posttest pattern. The research was conducted at the North Bogor Community Health Center in April-June 2023 on 66 elderly people taken using a purposive sampling technique. The questionnaire is used to look at characteristics, vaccine history, assess respondents' knowledge, attitudes and behavior regarding the COVID-19 situation. Data collection went through three stages, namely pre-test distribution, peer group method using module-19 and post-test distribution. Bivariate analysis with dependent t-test using SPSS 23.0 software. Results: The majority of elderly people were aged 60-74 years (90.9%) and there was a significant increase (p<0.05) in the average score of knowledge, attitudes and behavior of elderly people before and after being given the intervention. The average knowledge score before intervention was 69.60 ± 9.51 and after intervention 80.30 ± 10.29, attitude score before intervention 80.43 ± 9.61 and after intervention 85.25 ± 11.99, and behavior score before intervention 86.29 ± 14.67 and after intervention 90.35 ± 10.34. Conclusion: implementation of the model with intervention modules is effective in increasing knowledge, attitudes and behavior. It is hoped that with this increase, elderly people will be able to receive the COVID-19 vaccine.
Keywords: Acceptance of the COVID-19 Vaccine; Attitudes; Behavior; knowledge; Module; Peer group

The Implementation of Quick Wins Blood Services Program in Efforts to Prevent Maternal Deaths Due to Hemorrhage in East Lombok Regency - Alfina H. Ummah; Sudirman Nasir; Suriah; Shanti Riskiyani; Syamsuar; Lalu M. Saleh; Anwar Mallongi (Pharmacognosy Journal, 2024)
The Quick Wins Blood Service Program was launched by the Ministry of Health to accelerate the fulfillment of blood needs for pregnant women with hemorrhage complications by integrating public health centers, referral hospitals, and blood transfusion units. Maternal hemorrhage deaths could be prevented if more voluntary donors regularly donated blood. Purpose: This study aims to analyze the Quick Wins Blood Service Program implementation in East Lombok Regency. Methods: This is qualitative research with a case study design. Data were collected by interviewing 11 informants from the blood transfusion unit of the hospital, the East Lombok Red Cross, Public Health Centers, the East Lombok District Health Office, and high-risk pregnant women. Results: Health services for pregnant women have been adequately provided. However, some midwives do not conduct home visits and only wait for patients at the posts (polindes) in some areas or only do examinations at the integrated health services posts (Posyandu). Blood services have been good, but more blood stocks are often needed due to a lack of donor interest. Preparing prospective donors for pregnant women in the Quick Wins program is not always carried out because pregnant women and their families assume that blood is always available at referral hospitals. Conclusion: The Quick Wins Blood Service Program has been well implemented but needs to be more optimal.
Keywords: Blood Transfusion; Hemorrhage; Maternal Death; Pregnancy

The Influence of Organizational Culture and Group Climate on Innovation Readiness at Hasanuddin University Hospital - Michele Wijaya Oei; Syahrir A. Pasinringi; Andi Indahwaty Sidin; Noer Bahry Noor; Fridawaty Rivai; Healty (Pharmacognosy Journal, 2024)
Innovation is considered a competitive mechanism for organizational performance and success, and is considered an important instrument for adapting to the ever-changing business environment. This research aims to analyze the influence of Organizational Culture and Group Climate on Innovation Readiness at Hasanuddin University Hospital. The type of research carried out was quantitative research using an observational study with a cross sectional study design. The sample in this study was employees at the hospital, totaling 123 respondents. The research results show that there is a direct influence of organizational culture on innovation readiness, there is a direct influence of group climate on innovation readiness. It is recommended that hospital management be open to new ideas and encourage their employees to be more open and accept input from other employees. This will greatly encourage the emergence of innovation, improve the climate in positive work teams, such as participative, open and safe, will motivate employees to show Innovative and improving behavior creates or adopts new ideas, products, services, programs, technologies, policies, structures or new administrative systems.
Keywords: Group Climate; Hospital; Innovation Readiness; Organizational Culture

Inhibitory Mechanisms of Soybean Extract on the Development of Breast Cancer Through Modulation of Cellular Immune Response - Andi Muh. Maulana; Kusmardi Kusmardi; Erni Hernawati Purwaningsih; Andon Hestiantoro; Taifo Mahmud; Heri Wibowo; Bambang Pontjo Priosoeryanto; Primariadewi Rustamadji; Numlil Khaira Rusdi (Pharmacognosy Journal, 2024)
Background: Breast cancer is the most frequently diagnosed cancer in women worldwide. Consumption of soy products has been reported to reduce the incidence of and mortality rate for some cancers, including breast cancer. However, there are limited in vivo studies on the inhibitory effect of soybean extracts on breast cancer. Objectives: To examine the effect of soybean extracts on breast cancer cellular immunity and to determine the role of CD4+ and CD8+ T cells in the development and outcome of breast cancer. Material and Methods: Rat were induced with DMBA 11 times to get a breast cancer model. A soybean extract was given at different doses starting one week before DMBA induction and continued until the end of the study. At the end of the study, peripheral blood was collected, and the lymphocytes were examined using flow cytometry. Results: The phytochemical screening of soybean extract, using the Q-TOF LC/MS method, detected four bioactive components from the isoflavone and saponin groups. The incidence of tumor formation in the NeC, SE-D250, SE-D500, and SE-D1000 groups was 100%, 83%, 33%, and 33%, respectively. The highest proportion of CD4+ T cells was found in the NeC (69.35%), while the lowest was in the SE-D1000 (63.75%). The highest and lowest proportions of CD8+ T cells were found in the SE-D1000 and NeC groups, at 35.95% and 31.15%, respectively. Conclusions: The soybean extract was able to reduce the incidence of breast tumor formation in DMBA-induced rat in a dose-dependent manner. The soy extract group's CD4+/CD8+ ratio was close to that of healthy rats compared to the DMBA-induced group without soy extract. A lowered CD4+/CD8+ ratio is followed by a lower risk of tumor formation.
Keywords: Breast cancer; CD4+; CD8+; Cellular immune response; Soybean extract

The In-Vivo Assessment of the Effect of Traditionally Used Asparagus laricinus Extracts for Anticancer on the Kidney, Liver, and Spleen of Rats - Sekobane Daniel Mokgawa; Pakiso Moses Makhoahle; Samson Mashele (Pharmacognosy Journal, 2024)
Introduction: The plants has been a fertile source of revealing novel molecules discovered by sophisticated techniques for drug discovery. The present research was triggered by the increase in the use of Asparagus laricinus as home remedy, with a lot of studies done invitro on the plant evaluating possible toxic effects of the dried roots extracts using Sprague Dawley rats as animal models was needed. The objectives of the study was to investigate deviations effects in haematology and histology parameters, on the liver, kidneys and spleen tissues of animals exposed to aqueous and ethanolic extracts of Asparagus laricinus roots. Methods: Interfaculty Animal Ethics Committee approval was obtained from the Faculty of Health Sciences at the University of the Free State. All experimental work was performed in Animal Research Unit at the University of the Free State, Bloemfontein, South Africa. The supernatant of dried plants was filtered, and the ethanol removed completely under vacuum. The aqueous sample was lyophilized to obtain dried powdered material. The powdered plant material was dissolved in distilled water to prepare 2%, 10% and 20% concentration. 54 Sprague Dawley rats (180g and 250g), both male and female, were divided into two groups of 24 and 30 rats for aqueous and ethanolic extracts respectively. The aqueous group was further divided into four subgroups of 6 rats which were exposed to 2%, 10% and 20% extracts and the final group were controls (unexposed). The ethanolic group was divided into five subgroups of 6 rats which were exposed to increasing doses of 50, 100, 200 and 400mg/kg/day extracts and the last group were controls (unexposed). The aqueous extracts were administered to the three subgroups for eight weeks ad libitum while the control group was exposed to tap water. Ethanol extracts were administered daily over a period of two weeks through gavage and the control group was administered water through gavage as well. Blood samples were collected, animals were sacrificed, and organs/tissues excised for histological assessment. Results: Haematological tests were selected as indicators of the damage to the tissue of organs, including the liver, kidney, and spleen. Comparison of treatment groups (n=6) and controls (n=6) across all ethanol extracts showed significant differences in the starting median change in weight at the 200g/kg/day dosage, as well as the median termination weight at 400g/kg/day. There were no statistical differences between the treatment groups and controls with regard to the rest of haematological variables. Comparison of the controls (n=6) and treatment groups (n=6) revealed an average median change in weight of slightly above 50g over the entire eightweek period of experimentation with aqueous extracts. The Histological evaluation could not reveal any pathological changes in both the aqueous and ethanolic extracts across all levels of dosage. Discussion and conclusion: Haematological results could not show any patterns in abnormalities although we observed statistically significant results on few parameters. Histologically, no pathological changes were observed. In conclusion, we summarize that the toxicological evaluation of Asparagus laricinus extracts may be considered relatively free of toxicity when given orally, as it did not cause death, damage, or inflammation to the tissues, nor produced any remarkable haematological adverse effects in both the male and female Sprague Dawley rats.
Keywords: Asparagus Laricinus; Haematology; Histology; In vivo; Sprague Dawley rats

Measuring Adolescent Mental Health and the Effect on Junevile Deliquency Behavior Tendencies. Case Study in Tana Toraja - Librawaty Sara Tangibali; Muhammad Syafar; Ridwan M. Thaha; Sudirman Nasir; Hasanuddin Ishak; Sukri Palutturi; Anwar Mallongi (Pharmacognosy Journal, 2024)
Background: Adolescence is a transitional phase of development from childhood to adulthood, involving various changes in the biological, cognitive, and socio-emotional aspects of the adolescent. These changes often lead to confusion in establishing their identity, which can result in behaviors that are not in line with their developmental responsibilities. The tendency towards delinquent behavior in adolescents is one of the forms of behavior that emerges during adolescence due to the dysfunction of various aspects within the adolescent. The presence of dysfunction in these aspects of the adolescent indicates that the adolescent is experiencing mental disorders. The aim of this study is to analyze how the mental health of adolescents influences their tendency to engage in delinquent behaviors. Method: Using a quantitative method with descriptive statistics and testing assumptions using multiple linear regression analysis. Result: Based on the characteristics of adolescents aged 14 to 18 years, it was found that the mental health condition of adolescents has an impact on the tendency of delinquent behavior in adolescents, with a value based on the linear regression model of 0.000 < 0.05, indicating a significant level. Conclusion: The non-impulsive behavioral aspect is the most significantly influential factor on the tendency of adolescent delinquent behavior with a value of p < 0.05.
Keywords: Adolescent; Juneville delinquency tendency; Mental health; Self-Control

Microbial Quantitative Risk Assessment in Springs as Community Drinking Water Sources in the Banggai Islands Karst Area, Central Sulawesi - Ferdy Salamat; Anwar Daud; Agus Bintara Birawida; Amran Achmad; Muh. Syafar; Ridwan Amiruddin; Stang; Langgeng Wahyu Santoso; Anwar Mallongi (Pharmacognosy Journal, 2024)
The Banggai Karst Area of the Islands of Central Sulawesi Province is dominated by the appearance of the Karst Ecosystem, which covers 97% of the total area, so it is very vulnerable to pathogenic bacterial contamination. This type of research is an observational study with a Cross-Sectional design with an Environmental Health Risk Assessment (EHRA) approach method to assess or forecast the amount of human health risk caused by exposure to environmental hazards. Quantitative microbial risk assessment (QMRA) determines or predicts the human health risk caused by exposure to environmental hazards from pathogenic bacterial contamination. In this study, we collected 230 human samples and four samples of springs as a source of community drinking water, namely Paisu Lalomo Spring in South Buko District, Paisu Taabak in Liang District, Paisu Olulan in North Bulagi District and Paisu Sinangkal North Tinangkung District. The examination results on four springs as a source of community drinking water contained one sample with the highest probability of infection (Pinf) of 3.92 x10-4, namely Coliform bacteria in Paisu Lalomo springs. Furthermore, the annual chance of infection (Pinf / year), the highest pathogenic bacteria, was also found in Paisu Lalomo spring samples, namely in coliform bacteria with the same value (Pinf / year) = 1.32×10-1. However, examination of other bacteria also showed that all positive springs contained coliform bacteria and Enterococcus exceeding the specified limit (1x10-4). Risk management is needed to control risk factors that can cause health problems due to consuming and utilizing springs as a source of drinking water.
Keywords: Karst; Pathogenic bacteria; QMRA; Springs

New Insights of Oxidative Stress and Thalassemia May Lead to Antioxidant Therapy - Kassim SA Al Neaimy; Maes MK Alkhyatt; Israa A Jarjess (Pharmacognosy Journal, 2024)
Background: Because of chronic hemolysis, thalassemic patients are under oxidative cell injury caused by secondary iron overload. This provokes oxidative damage to the cellular membranes of organs that accumulate excess iron. Several researchers studied the oxidative stress in patients with thalassemia during chelation therapy and repeated blood transfusion periods, and they found that β-thalassemia patients are under oxidative stress, but they did not focus on before the chelating therapy period. Objective: To evaluate the total antioxidant capacity (TAOC) and oxidative stress (OS) in newly diagnosed patients with β-thalassemia before chelating therapy. Methodology: In the present case-control study, twenty patients newly diagnosed with β-thalassemia before receiving chelating agents, and another 30 healthy individuals, sex-matched with patients, considered as a control, were included in the study. Total antioxidant capacity (TAOC) and Malondialdehyde (MDA) were assessed in the studied groups. Results: The TAOC values of the thalassemic group (35±0.11 u/ml ) were significantly (p<0.001) lower than that of the control group (79±7.2 u/ml). MDA values of the thalassemic group (7.9 ±2.35nmol/l) were significantly (p<0.001) more than that of the control group (0.57±0.25 nmol/l). Conclusion: The present study demonstrated that patients with β thalassemia have decreased values of TAOC, and increased values of MDA when compared with the control group.
Keywords: Antioxidants; Oxidative stress; Thalassemia

Occupational Diseases in Female Workers in the Industrial World, Indonesia: Hazard Types and Exposure Mechanisms – Literature Review - Mirawati Tongko; Yahya Thamrin; Syamsiar S. Russeng; Lalu Muhammad Saleh; Sukri Palutturi; Ridwan Amiruddin; Irwandy; Anwar Mallongi; Maria Kanan; Fahrudin Lahay; Almustari (Pharmacognosy Journal, 2024)
Background: Occupational diseases are diseases caused by work and the work environment. Specifically gender, women have a double burden, namely domestic work in the household and the burden of being a breadwinner who works outside the home, women are also faced with reproductive tasks, namely pregnancy, childbirth and breastfeeding, and bioanatomically women have physical strength different from men, so that women are specifically more vulnerable to health risks due to their work, both physically, mental and social. This research will review the types and mechanisms of the spread of Occupational Diseases (OD) in female workers in the industrial world. Methods: The writing of this research was carried out using the Literature Review method which is a literature review method in which scientific articles are selected by researchers related to the research topic. Results: According to the results of the study, the specific risk of occupational diseases in the female worker community is pregnancy and childbirth disorders, Anemia and Sexuall Transmitted Disease (STD), while other accompanying diseases are Pulmonary Obstruction Disease, Tinea pedis, Neurotoksic Symptoms, Noice Induce Hearing Loss (NIHL), Low Back Pain (LBP), MSDs, and Work Stress. Conclusion: Every female worker has a susceptibility to occupational diseases according to the conditions and work environment experienced. The industry must pay attention to programs to improve occupational health by making anticipatory efforts so that occupational diseases do not become a life threat to female workers.
Keywords: Female Worker; Hazard; Industry; Occupational Disease

Pharmacognostic Profile and Antidiabetic Activity of Eleutherine bulbosa Mills. Bulbs from East Kalimantan, Indonesia - Herman Herman; Arsyik Ibrahim; Junaidin Junaidin; M. Arifuddin; Baso Didik Hikmawan; Siska Siska; Tahyatul Bariroh; Reza Yuridian Purwoko; Lizma Febrina; Muhammad Faisal; Iswahyudi Iswahyudi; Marissa Angelina; et al. (Pharmacognosy Journal, 2024)
Background: Eleutherine bulbosa (Mill.) Urb., known as Bawang Dayak, is commonly found growing in Kalimantan, Indonesia. The characteristics of the environment in which the plant grows greatly influence the characteristics of the plant and its biological activity. Objective: This study aimed to determine the pharmacognostic profile and evaluate the antidiabetic activity of E. bulbosa bulb extract obtained from Kota Bangun, Kutai Kertanegara, Indonesia. Methods: The dried powdered of E. bulbosa bulbs was extracted using two different methods (maceration and microwave-assisted extraction). The evaluation of the sample fluorescence and non-specific parameters was done. The antidiabetic activity of the extracts was conducted using streptozocin-induced mice and histopathology analysis. Results: The fluorescence evaluation indicated various secondary metabolites contained in E. bulbosa simplicia. Non-specific parameters include drying loss (8.83±0.2611%), water content in simplicia (8.04±0.7481%), specific gravity (1.04±0.0058%), total ash content (20.3±0.1583%), acid insoluble ash content (1.70±0.0608%), ethanol content in extract (0.99±0.0017%), lead content (0.010 mg/g), cadmium (0.091 mg/g), Arsenic (0.0003 mg/g), aflatoxin (not detected), and mold/yeast (0.8 × 105 colonies/g) meet the established regulations. Meanwhile, the antidiabetic assay showed that both extracts decreased the blood glucose levels in mice during the 14 days of treatments in a dose-dependent manner. This activity is correlated with the increased repair of pancreatic beta cells as observed by histopathological analysis. Conclusion: The dried bulbs of E. bulbosa obtained from Kota Bangun, Kutai Kertanegara, East Kalimantan, Indonesia meet the standards set as raw materials for antidiabetic herbal medicines.
Keywords: Antidiabetic activity; Bawang Dayak; contaminant levels evaluation; Eleutherine bulbosa (Mill.) Urb; pharmacognostic profile

Phytochemical Screening, Thin Layer Chromatography and Fourier Transform Infra-Red Spectroscopy Analysis of Eleutherine Bulbous (Mill.) Urb Bulb Extract - Lokot Donna Lubis; Muhammad Fidel Ganis Siregar; Farhat; Iqbal Pahlevi Adeputra Nasution; Dedy Syahrizal; Kamal Basri Siregar; Putri Chairani Eyanoer; Ichwanul Adenin; Muhammad Rusda; Mustafa M. Amin (Pharmacognosy Journal, 2024)
Background: Many traditional medicinal plants were studied for their phytochemical components. Dayak onions (Eleutherine bulbous (Mill.) Urb) is one of traditional medicinal plant in Indonesia. Analysis of the phytochemical compounds in the extract of the plant would help in determining various biological activities of the plant. Objective: This study was primarily aimed to identify the phytochemical profile of Dayak onions extract and its fractions. Methods: The Dayak onion bulbs were collected from Pancur Batu District, North Sumatra Province, Indonesia. Multiple solvents used to for extraction and analysis of phytochemical compound by chemical reactions, thin layer chromatography (TLC) profiling, and Fourier Transform Infrared Spectrophotometer (FTIR) profiling. Results: The phytochemical screening of Dayak onions ethanol extract showed it contained alkaloids, flavonoids, glycosides, tannins, and triterpenes/ steroids. The n-hexane fraction contained flavonoids and triterpenes/steroids, while the ethyl acetate fraction contains flavonoids and glycosides. The water fraction contained alkaloids, flavonoids, glycosides and tannins. By TLC profiling, the bioactive compounds in ethanol extract of the Dayak onion contain steroids, tannins, flavonoids and alkaloids. The bioactive compound in ethyl acetate fraction contains steroids, tannins, triterpenes, flavonoids and glycosides. The bioactive compound in n-hexan fraction contains steroids. The bioactive compound in water fraction fraction contains steroids and tannins. The FTIR demonstrated the presence of C-H alkanes, =C-H alkenes (aliphatic), C-O alcohol, ether, esters and carboxy acid in the ethanol extract, en-hexan fractions and ethyl acetate fractions. Conclusion: The phytochemical qualitative profiling showed that Dayak onion bulb as a rich source of bioactive compounds and have medicinal potential as therapeutic agent. A higher content of bioactive compounds of Dayak onion bulbs found in the ethanol and ethyl acetate extracts. The FTIR demonstrated the presence of C-H alkanes, =C-H alkenes (aliphatic), C-O alcohol, ether, esters and carboxy acid in the ethanol extract, enhexan fractions and ethyl acetate fractions.
Keywords: Dayak onion; Eleutherine bulbous; Extract; Fraction; FTIR; TLC

Poikilodermatous Mycosis Fungoides: A Rare Case Treated with Low-Dose Methotrexate - Eva Krishna Sutedja; Frizam Dwindamuldan Sutisna; Endang Sutedja; Kartika Ruchiatan; Yogi Faldian; Laila Tsaqilah; Hermin Aminah Usman; Yovan Rivanzah (Pharmacognosy Journal, 2024)
Mycosis fungoides (MF) is a subtype of cutaneous T-cell lymphoma (CTCL) and a primary cutaneous lymphoma. Poikilodermatous MF (PMF) is a rare clinical variant of MF. Systemic chemotherapy, such as methotrexate (MTX), may be administered as monotherapy or in combination at low doses in MF. A 64-year-old man with PMF has been reported. History and physical examination revealed hyperpigmented and hypopigmented macules on the chest, abdomen, both arms, back, and upper legs, along with erythematous macules on the right medial thigh. The diagnosis of PMF is based on clinical manifestations and histopathological features of atypical lymphocyte cell infiltration in the epidermis. In addition, immunohistochemical examination also supports the MF diagnosis. The patient received chemotherapy consisting of monotherapy with MTX at low doses. During the third month of observation following MTX therapy, a few of the hyperpigmented and hypopigmented macules appeared to have diminished; some of the erythematous macules had transformed into hyperpigmented macules, and there were no new lesions. The purpose of therapy is to control the disease. Administration of low-dose MTX as a monotherapy may result in clinical improvement, but further observation is necessary. PMF may have a better prognosis than other clinical variants of MF.
Keywords: Cutaneous T-cell lymphoma; Methotrexate; Mycosis fungoides; Poikilodermatous mycosis fungoides

Predictive Simulation and Functional Insights of Serotonin Transporter: Ligand Interactions Explored through Database Analysis - Irzan Nurman; Ninik Mudjihartini; Nurhadi Ibrahim; Linda Erlina; Fadilah Fadilah; Muchtaruddin Mansyur (Pharmacognosy Journal, 2024)
Through its ability to facilitate the absorption of serotonin into presynaptic neurons, the serotonin transporter, also known as SERT, an essential component in the control of neurotransmission. To discover SERT possible therapeutic application, it is essential to have a solid understanding of its dynamic behavior, ligand interactions, and functional consequences. Within the scope of this investigation, the predictive simulations is crucial to investigate the complexities of SERT to gain a fresh understanding of its operation. We use the 6AWN model to describe the sequence and simulate the behavior of SERT in silico. Within this simulation, we anticipate the conformational changes of SERT and its reaction to ligand binding with paroxetine, cholesterol, dodecyl-beta-D-maltose (DDM), and sodium hydrogen ion. We discover critical residues that are crucial in the interaction between ligands and proteins. They have paroxetine binding to I.172, I.172, Y.176, and F.341 are examples of hydrophobic interactions. Example of hydrogen bonds include A.96 and pi-stacking: F.341. The blockage of the serotonin transporter is the principal mechanism of action that paroxetine has. Cholesterol interacts with SERT W.500, W.500, W.500, W.500, L.504, and A.507, and it also interacts with the outward-facing conformation of this transporter in two different ways. In general, cholesterol interacts with SERT and ligands to stabilize their optimal activity and structure. DDM contact with SERT is also a part of this interaction. R.104, D.328, E.494, Y.495, G.498, P.499, T.503, F.556, L.557, S.559, P.561, Y.579, G.582, T.583, and F.586 are the numbers that are currently in use. Within the context of glucosyl transfer processes, DDM has been utilized as an acceptor. And the interaction of Na with SERT S.263, which causes a change in the structure of SERT. Serotonin transporters are present in the environment.
Keywords: Database Analysis; Functional analysis; Predictive in silico; Serotonin Transporter

Punicalagin Opposes Gentamicin Nephrotoxicity in Rats: Role of Nrf2 and NF-κB Pathways - Amr A. Fouad; Moataz Mohamedalhasan Ali; Mostafa Abdel-Hamid (Pharmacognosy Journal, 2024)
Background: Oxidative stress, inflammation, and apoptosis are implicated in gentamicin (GEN)-induced nephrotoxicity. Punicalagin (PNG) possesses antioxidant, anti-inflammatory, and antiapoptotic effects. Objective: The aim of the present research was to investigate the possible defensive effect of PNG against nephrotoxicity caused by GEN in male Sprague-Dawley rats. Materials and Methods: GEN (80 mg/kg/day, i.p.) was administered for 8 days. Treatment with PNG (25 mg/kg/day, p.o.) for 10 days, began 2 days before GEN insult. Results: PNG significantly decreased serum creatinine, and malondialdehyde, tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, nuclear factor-κB p65 (NF- κB p65), and cleaved caspase-3 activity in the kidneys of GEN-challenged rats. PNG also significantly increased renal catalase, reduced glutathione, and nuclear factor erythroid 2-related factor 2 (Nrf2) in rats received GEN. Additionally, PNG markedly attenuated the histopathological kidney tissue injury caused by GEN. Conclusion: PNG guarded against GEN-induced kidney damage in rats through its antioxidant, anti-inflammatory, and antiapoptotic effects, and by modulating the balance between Nrf2 and NF-κB pathways.
Keywords: Gentamicin; Kidney; Punicalagin; Rats

Quantification of Four Phytochemical Parameters of Imperata cylindrica Leaves to Promote Its Use as A Medicinal Plant - Diah Dhianawaty; Resti Gradia Dwiwina; Wulan Mayasari; Achadiyani; Ruslin (Pharmacognosy Journal, 2024)
Introduction: The benefits of Imperata cylindrica (I. cylindrica) leaves and their relationship to the content of phytochemical compounds have been widely studied. The aim of this study was to promote I. cylindrica leaves from Kertajati Subdistrict in Majalengka Regency in their use as a medicinal plant based on the four parameters, namely the content of total phenolic content, total flavonoid content, total tannin content, and antioxidant activity. The four parameters that become the basis for the benefits of the leaves were compared with the four parameters of the roots. Methods: Quantification of total phenolic content, total flavonoid content, total tannin content, and antioxidant activity used Folin-Ciocalteu, aluminium chloride, Fe(III) chloride and 1.10-Phenanthroline, and 1,1-diphenyl-2-pikrilhidrazil (DPPH) methods, respectively. All methods used visible spectrophotometric method. Results: The percentages of total phenolic content, total flavonoid content, total tannin content, and IC50 of antioxidant activity of I. cylindrica leaves extract were 9% (GAE), 2.1% (QE), 5.6% (TAE), and 100.5 ppm, respectively. Then, the percentages of I. cylindrica roots extract showed the percentages of total phenolic content, total flavonoid content, total tannin content, and IC50 of antioxidant activity were 5.8% (GAE), 0.64% (QE), 3% (TAE), and 241 ppm, respectively. Conclusion: The extract of I. cylindrica leaves contained the phenolic compound, namely flavonoids, tannins and other phenolic compounds, and had antioxidant activity. All parameters have been reported to have positive effect on health. Therefore, the leaves of I. cylindrica from Kertajati Subdistrict are discovered to have effects on health which are suitable to be promoted as a medicinal plant.
Keywords: antioxidant activity; Imperata cylindrica leaves; total flavonoid content; total phenolic content; Total Tannin content

Safety Assessment of Oral Lysiphyllum strychnifolium Aqueous Extract in Healthy Volunteers - Piracha Jumpa-ngern; Parunkul Tungsukruthai; Chuntida Kamalashiran; Somboon Kietinun; Kesara Na- Bangchang; Kusuma Sriyakul (Pharmacognosy Journal, 2024)
Background: Lysiphyllum strychnifolium (LS), widely known as Ya nang daeng in Thailand, is a traditional herbal remedy that has long been used to promote health and treat diverse health conditions, especially detoxification, by alleviating the severity of symptoms and lowering the risks associated with toxic exposures. Although it is extensively used in Thailand, human safety studies have been lacking. Thus, this study aimed to examine the safety of using LS capsules in healthy participants through a Phase I clinical trial. Objective: This study aimed to investigate the safety of aqueous extract of LS in twenty-four healthy Thai participants. Method: The participants were received 1,000 mg of LS aqueous extract each morning before their meals for seven days. All participants were examined safety assessment including history taking, physical examination, and laboratory tests at day 0, 8 and 14 (follow-up). Results: The findings showed that there were no significant side effects or abnormalities found during the history taking, physical examination, or laboratory evaluation. Particularly, when compared to baseline, participants who received LS experienced statistically significant reductions in blood sugar, triglyceride, LDL cholesterol, and creatinine (P < 0.05), but still within normal ranges. Conclusions: Dietary supplementation with 1,000 mg of LS aqueous extract per day may have a beneficial effect on blood sugar and cholesterol management while remaining safe for healthy people.
Keywords: Clinical study; Healthy volunteers; Lysiphyllum strychnifolium; Safety; Yanang Daeng

Screening and Identification of Metabolites from Sambiloto (Andrographis paniculata) Ethanol Extract for Pro-Inflammatory Cytokines Inhibitory through In Silico and In Vitro Approaches - Evul Winoto Lukito; Dyah Iswantini; Budhi Antariksa; Mohamad Rafi; Setyanto Tri Wahyudi (Pharmacognosy Journal, 2024)
Objective: Andrographis paniculata has long been a traditional medicinal plant in Indonesia. This study is intended to evaluate the anti-pro-inflammatory cytokines of 98% ethanol extract of A. paniculata by in vitro and in silico approaches. Inhibition of pro-inflammatory cytokines is also one of the therapies in treating COVID-19. Methods: The molecular docking approach was utilized as a first screening to evaluate the potential for suppression of macrophage cell activation; an ADMET prediction test was performed to determine the pharmacological, pharmacokinetic, and toxicity as a therapeutic target. TNF-α, IL-1ß, and IL-6 levels were measured using an ELISA method to investigate anti-cytokine pro-inflammatory activity in LPS-induced RAW 264.7 macrophage cells. LC-MS/MS was used to identify additional metabolite compounds. Results: Ethanol extract containing particular metabolites 14-Deoxyandrographoside and 14-Deoxy-17-hydroxyandrographolide inhibited TNF-α and IL-1β by 100% and IL-6 by 85.59%, respectively. While compared to the Dexamethasone molecule as a positive control, preliminary screening and ADMET prediction for the metabolite compound 14-Deoxyandrographoside exhibited relatively high binding stability to the CD14 receptor by -7.5 kcal/mol and was safe against various ADMET indications. Conclusions: This study reveals that the compound 14-Deoxyandrographoside in pure ethanol extract is a potential anticytokine agent candidate for treating pro-inflammatory cytokines, including COVID-19 infection.
Keywords: LC-MS/MS; Molecular docking; Pro-inflammatory cytokines; Sambiloto

Stunting and Family Socio-Cultural Determinant Factors: A Systematic Review - Henniyati Harahap; Aminuddin Syam; Sukri Palutturi; Muhammad Syafar; Anto J. Hadi; Haslinah Ahmad; Hapiz Arlanda Sani; Anwar Mallongi (Pharmacognosy Journal, 2024)
Background: Stunting is a major public health problem in many developing countries including Indonesia and several ethnic groups have a very high prevalence of stunting which is caused by socio-cultural factors in the family. Objective: This study aims to review family social and cultural factors in relation to stunting. Method: This research uses a systematic review using the PRISMA method. There are 5 steps in this method namely determining the literature topic, searching for sources, selecting relevant sources, grouping and analyzing, and summarizing published research articles on family social culture on stunting published between 2018-2022 in the online article databases PubMed, Proquest, Scopus, Google Scholar and Sciencedirect. Data collection techniques by entering keywords in the database, keywords used: family social culture, intervention AND family environment risk of stunting*social demographics OR health education OR family empowerment OR maternal nutritional behavior OR family empowerment AND stunting. Screening consists of title, abstract, year of research, and methods used. Next, sorting the articles according to the predetermined inclusion criteria. The inclusion criteria in this study are: (1) Quantitative type of research with primary data; (2) Contains research results on socio-cultural factors that influence stunting in children; (3) the year the research was published in the last five years (2018-2022), (4) the sample used was families (children), (5) the text of the article was in English. A total of 18 of 1,544 articles met the inclusion criteria for this review material. Results: This study shows that from the 18 selected articles it was found that stunting is caused by socio-cultural and family demographics, family nutritional behavior, and the family environment at risk of stunting. In addition, a model of health education and family empowerment based on family socio-culture was obtained. Conclusion: This study found that family social culture is a significant determinant of stunting.Therefore, further research needs to be carried out to complete our understanding of the complexity of the stunting problem.
Keywords: Family Behavior; Health Education; Social Culture; Stunting

Test of Antihyperuricemia Activity of Methanol Extract of Plantain Peel (Musa Paradisiaca Var. Sapientum) Against Caffeine- Induced Mice (Mus Musculus) - Ratna Umi Nurlila; Jumarddin La Fua; Toto Surianto S; Titi Purnama; Muh Ikhsan Idrus; Armayani; Anwar Mallongi (Pharmacognosy Journal, 2024)
Background: Hyperuricemia occurs when serum uric acid levels rise above the normal range, typically exceeding 7.0 mg/dl for adults and 6.0 mg/dl for women. This condition can lead to various health issues, including gout, kidney stones, and renal failure. Objective: This study aims to verify the effectiveness of the methanol extract from plantain peel (Musa paradisiaca var. sapientum) in reducing uric acid levels in mice (Mus musculus) and to establish the optimal dosage for maximum efficacy. Materials and Methods: This analytical experimental research utilized a paired sample T-test Pretest-Posttest design. The mice were divided into five groups: a Negative Control of Na.CMC 0.5%, a Positive Control of allopurinol at 100 mg/kg body weight, and groups receiving plantain peel methanol extract at 100 mg/kg, 200 mg/kg, and 400 mg/kg body weight. Uric acid levels were measured initially, post-caffeine induction on day 7, and post-extract induction on day 14. Results: The study found that plantain peel methanol extract exhibited an anti-hyperuricemic effect in male mice. Among the dosages tested, 400 mg/kg body weight of the plantain peel methanol extract was the most effective in reducing uric acid levels, followed by the 200 mg/kg and 100 mg/kg doses. Conclusion: Plantain peel methanol extract is effective in reducing uric acid levels in male mice, with 400 mg/kg being the most efficacious dosage. This suggests potential for further exploration of plantain peels as a treatment for hyperuricemia.
Keywords: Anti-hyperuricemic effect; Hyperuricemia; Methanol extract; Mus musculus; Optimal dosage; Plantain peel; Uric acid levels

Unlocking the Potential of Stingray (Dasyatis sephen) Extract: A Novel Investigation into EPA and DHA Effects on Lipid Metabolism in High-Fat Diet-Fed Wistar Rats - Sihning EJT; Ni Komang Sri Dewi Untari; Dian Maria Pia (Pharmacognosy Journal, 2024)
Introduction: Dyslipidemia is a complex disturbance in lipid metabolism with notable implications for cardiovascular health. Considering the burden of cardiovascular disease (CVD) on global mortality, lipid regulation should be eminently prioritized. Stingray (Dasyatis sephen) is one of the natural resources believed to be rich in lipid-regulating compounds: eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). We aimed to investigate the impact of the stingray extract on the lipid metabolism of Wistar rats subjected to a high-fat diet. Methods: We adopted a true experimental design with three experiment groups: a negative control (standard diet and distilled water), a positive control (high-fat diet and distilled water), and a specified treatment group (high-fat diet and stingray extract). Serum samples were collected after 28 days of treatment and analyzed for HDL and ApoB levels as dyslipidemia markers. Statistical analysis included one-way ANOVA for HDL and non-parametric Kruskal-Wallis tests for ApoB levels, followed by post-hoc analysis. Results: The results revealed worsening HDL and ApoB levels in the positive control group compared to the negative control group following a high-fat diet consumption. In contrast, the experiment group showed improvements in HDL and ApoB levels compared to the positive control group following the administration of the extract. However, only the dynamics in HDL level are significant (p<0.05), while the difference in ApoB levels among all treatment groups was not statistically significant (p>0.05). Conclusion: This study highlights the potency of D. sephen extract in regulating lipid metabolism despite the complexity of lipid metabolism regulation and the challenges of the conventional reliance on HDL and ApoB as exclusive markers of cardiovascular health.
Keywords: ApoB; Dasyatis sephen; HDL; stingray

Unveiling Potential Therapies: Molecular Docking Analysis of CAMKK2 and Its Mutant Variants with CAMKK2 Inhibitors in Indonesian Patients with HIV-Sensory Neuropathy - Ahmad Yanuar Safri; Salim Harris; Putera Dewa Haryono; Ariane Benina Budiwan; Eugenia Isadora; Aisyah Fitriannisa Prawiningrum; Fadilah Fadilah (Pharmacognosy Journal, 2024)
HIV sensory neuropathy (HIV-SN) is one among many complications that impair patients’ quality of life. Studies in Asian and African populations found that single nucleotide polymorphisms (SNPs) of calcium/ calmodulin-dependent protein kinase 2 (CAMKK2) influence the risk of HIV-SN. This study attempts to explain the influence of CAMKK2 mutations on HIV SN by studying bioinformatics interactions between CAMKK2, its mutants, and their inhibitors by molecular docking with AutoDock in order to observe their interactions with CAMKK2 inhibitors. Results showed that CAMKK2’s binding energy with its native ligand (ATP) is stronger than the mutant variant of CAMKK2MT85 and CAMKK2MT363. Conversely, interaction between CAMKK2 and its inhibitors (KN-93, STO-609, and trifluoperazine) have the lowest mean binding energy compared to CAMKK2MT85 and CAMKK2MT363. This indicates that the mutant variants have weaker interactions with the native ligand and the inhibitors, therefore disrupting the normal function of CAMKK2, its interactions with the inhibitors, while increasing the likelihood of HIV-SN.
Keywords: CAMKK2 inhibitors; HIV-SN; Molecular docking; mutation; SNP

Accessibility of Mother and Child Health Services to Stunting Incidence in Leti Island, Southwest Maluku District - Apia Unmehopa; Sukri Palutturi; Indar; Muhammad Alwy Arifin; Yahya Thamrin; Stang; Anwar Mallongi (Pharmacognosy Journal, 2023)
One of the nutritional problems that can worsen the quality of life of children in achieving growth and development is stunting. Stunting receives greater attention than other nutritional statuses because in addition to a higher prevalence it can also indicate something more serious than just short body size. This type of research is a quantitative study that uses analytic observational with a case control study design to see the effect of the independent variables on the dependent variable. The sampling technique in this study was simple random sampling with a total sample of 164 people, 82 people in the case group and 82 people in the control group. The results of this study indicate that there is an effect between exclusive breastfeeding and health services on the incidence of stunting, while for the basic immunization variable there is no effect on the incidence of stunting. Meanwhile, based on the multivariate test, it was found that the variable that had the most influence on the incidence of stunting was health services. As for suggestions for this research, it is hoped that the Health Service will further improve health services to monitor the growth of toddlers and for mothers of toddlers to visit health services regularly.
Keywords: Growth.; Health Services; Mother; Stunting; Toddlers

Activity of Moringa Oleifera Lam on Liver Function and Histology in White Male Rats - Yufri Aldi; Aisyah Aisyah; Rahmad Abdillah; Aditya Alqamal Alianta (Pharmacognosy Journal, 2023)
Introduction: Moringa oleifera Lam has many pharmacological activities, such as immunostimulants. Aim: This study aims to determine the safety profile of repeated use and routine use of Moringa oleifera Lam on liver function and histology. Material and Method: This study used 36 male white rats as test animals. Moringa leaves were administered orally as standardised ethanol extract at doses of 7, 21, and 140 mg/kg BW for 21 days. The liver function observed was the activity of the ALT enzyme. On days 8, 15, and 22, three animals from each group had their blood collected through the orbital sinuses of the eyes and sacrificed to excise the livers. The parameters observed were ALT enzyme activity, liver ratio value, and histological damage of liver tissue. The two-way ANOVA was used to analyse the data of ALT enzyme activity and organ ratio values, followed by DMRT. In contrast, descriptive analysis was used to describe liver histological damage. Result: Test animals had no toxic symptoms or sudden death during the study. The results showed that ALT enzyme activity was significantly affected by the dose and duration of administration of the ethanol extract of Moringa leaves (p<0.05). However, on the organ ratio values, there were no significant effects of dose (p>0.05), but significantly affected by duration of administration (p<0.05). The liver histological descriptive analysis showed histological differences between the control and treatment groups. The 7 mg/kg BW and 21 mg/kg BW showed liver tissue with average to minimal damage. However, the dose groups at 140 mg/kg BW for 14 and 21 days showed changes in liver histology, although only minimal to mild impairment. Conclusion: It concluded that the administration of extract of Moringa oleifera Lam did not cause severe damage to the liver of white male rats.
Keywords: Alanine aminotransferase; Histology.; Moringa oleifera L.; Subacute toxicity

Activity of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) Extracts in Capsule Dosage Form to IgM and IgG Levels in Humans - Afriwardi Afriwardi; Adinny Julmiza; Salman Umar; Yufri Aldi (Pharmacognosy Journal, 2023)
Objective: Traditionally, Pegagan embun (Hydrocotyle sibthorpiodes Lam.) has been used to enhance the immune system. Previous studies confirmed that Hydrocotyle sibthorpiodes Lam. enhanced antibody titers and was practically non-toxic by animal tests. This study aimed to determine the effect of Hydrocotyle sibthorpioides Lam. extract on IgM and IgG levels in humans. Methods: The participants involved 20 healthy volunteers aged 20-25 years were divided into two groups with a placebo as a comparison. The capsules containing Hydrocotyle sibthorpiodes Lam extract were in the treatment group at a dose of 67 mg and the placebo group was given capsules containing lactose orally for three days. IgM and IgG levels were measured before and after consuming the capsules. The ELISA method tested IgM and IgG levels and detected them using specific antibodies that form complexes with enzymes and substrates. Results: The results showed the average baseline IgM level of 1.480 mg/mL and after consumption of 1.405 mg/mL for placebo. For Hydrocotyle sibthorpiodes Lam. extract capsules, the baseline was 1.492 mg/mL and 1.854 mg/mL after consumption. For IgG, before given 8.941 mg/mL and after given 8.710 mg/mL for placebo, while before given Hydrocotyle sibthorpiodes Lam. extract capsules 8.946 mg/mL and after given Hydrocotyle sibthorpiodes Lam. extract capsules was 10.234 mg/mL. Statistical results showed a significant increase in IgM and IgG levels after consuming Hydrocotyle sibthorpiodes Lam. extract capsules for three days (p<0.05). Conclusion: It can be concluded that 67 mg of Hydrocotyle sibthorpiodes Lam. extract capsules can increase IgM and IgG levels in humans.
Keywords: ELISA; Extract; Hydrocotyle sibthorpioides Lam; IgG; IgM; Volunteers.

Acute and Sub-acute Oral Toxicity Profile of Root Bark Methanol Extract of Carissa Edulis Vahl - Jane Wanja Mbiri; Kenneth Ogila; Patrick Kisangau; Michael Gicheru (Pharmacognosy Journal, 2023)
Background: Carissa edulis is widely used in traditional medicine to manage numerous ailments. However, few studies have assessed its toxicity. Therefore, this study aimed to determine acute and sub-toxicity levels of C. edulis methanol extract. Methods: In the acute toxicity probe, a limit test was conducted whereby the extract was given as a solo dose by gavage. The rats were observed for two weeks. The observations included mortality and changes in the general appearance and behavior of the experimental animals. The body weights of the rats were taken weekly. For the sub-acute toxicity probe, the rats received the extract daily at dosages 300, 520, and 900 mg/kg by gavage for 28 days. Body weights were also taken weekly. On day twenty-nine, the weights of the rats were taken, the rats were sacrificed, and blood was collected for biochemical and hematological analysis. Body organs were harvested, and their weights were taken. Results: The results of the acute toxicity probe showed that the extract didn’t cause mortality or toxicity signs throughout the study duration. The LD50 of the extract was therefore deemed to be above 2,000 mg/kg. The sub-acute toxicity probe results demonstrated that the extract, at all the tested dosages, didn’t cause mortality or affect the rats’ organ weights, body weights, or hematological and biochemical parameters throughout the study duration. Conclusions: In conclusion, the methanol extract of C. edulis is not toxic since it didn’t cause mortality or toxicity signs in both acute and sub-acute toxicity probes.
Keywords: Biochemical parameters; Body weights; hematological parameters; Organ weights.

Aedes Sp. Mosquito Resistance and the Effectiveness of Biolarvicides on Dengue Vector Mortality - Bambang Dwicahya; A. Arsunan Arsin; Hasanuddin Ishak; Firdaus Hamid; Anwar Mallongi (Pharmacognosy Journal, 2023)
Until now, dengue fever is still a public health problem in Indonesia. To control mosquito vectors, various strategic approaches have been promoted such as chemical control, biological control, resource reduction and public education. The four main classes that are widely used for mosquito control are organochlorines, organophosphates, pyrethroids and carbamates. A literature study was conducted to determine the extent of Aedes aegypti resistance to several insecticides in several countries including Indonesia. This study also reviewed the effectiveness of biolarvicides on the mortality of Aedes sp. All research that has been done in the last 10 years is included in this topic. In Brazil, Sri Lanka, China and Peru, there have been reports of resistance to Aedes sp. against delthamethrin, pyrethroid and temephos insecticides. Meanwhile in Indonesia (Semarang, Surabaya, Banten, DKI Jakarta, North Sumatra, Jambi, Bandung, Bogor, Makassar and Palu), Aedes sp. resistance occurs to insecticides of the pyrethroid, temephos, malathion, cypermethrin, and permethrin groups. Continuous monitoring of mosquito vector resistance status is very important in the effectiveness of dengue fever control. In addition, research on plants that can be used as biolarvicides is very useful for alternative control and improving public health. This review also provides better insight into the effectiveness of laboratory-tested plants as larvicides and plans for further research to be applicable to the community.
Keywords: Aedes sp mosquito; Biolarvicides.; Dengue fever; Insecticide resistance

Alkaloids from Pandanus amaryllifolius Roxb Leaf as Promising Candidates for Antidyslipidemic Agents: An in silico study - Martohap Parotua Lumbanraja; Kusnandar Anggadiredja; Hubbi Nashrullah Muhammad; Neng Fisheri Kurniati (Pharmacognosy Journal, 2023)
Introduction: The plant Pandanus amaryllifolius Roxb (pandan), has been shown to have antidyslipidemic potency. This study explored the potential of several alkaloids from pandan leaf as antidyslipidemia as well as their safety profile in silico. Methods: Analyses were carried out by studying the binding affinity of the alkaloids to 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, peroxisome proliferator activator receptor (PPAR) alpha and Niemann Pick C1 Like 1 (NPC1L1). The structures of the alkaloids were downloaded from the Pubchem database and optimized using the ChemDraw Professional 16.0 to obtain 3D structures in protein data bank (PDB) format. The in silico testing was based on the interactions of the alkaloids with the HMG-CoA reductase (PDB ID 1HW9), PPAR alpha (PDB ID 6LX4) and NPC1L1 (PDB ID 7DFZ) proteins, downloaded from the Research Collaboratory for Structural Bioinformatics (RSCB) PDB website (http://www.rcsb.org/pdb). The preparation of protein structures was performed using the Discovery studio 2021 client and Gromacs applications, while optimization of the 3D structure of the alkaloids was carried out with the ChemDraw professional 16.0. Finally, validation was completed using AutoDock application. The safety profile was assessed by pkCSM online tool. Results: The respective root mean square deviation (RMSD) values of the 1HW9, 6LX4 and 7DFZ proteins were 1.677, 0.918 and 1.706, respectively. The alkaloids pandanusine B, pandamarilactonine A, pandamarilactonine B had respective values of binding energy for HMG-CoA of -5.52, -5.51 and -5.46 kcal/mol. The binding energy of pandamarilactonine B, pandamarilactonine A and pandanamine for PPAR alpha were -9.14, -9.10 and -8.48 kcal/mol, respectively, with the corresponding energy for t NPC1L1 of -9.63, -9.71 and -8.54 kcal/mol. The toxicity tests indicated that the alkaloids were safe, pandamarilactonines had the highest LD50 (2.736 mol/ kg). Conclusion: The studied pandan alkaloids have potential antidyslipidemic activity by interacting with HMG-CoA reductase, PPAR alpha, and NPC1L1, with good safety profile.
Keywords: Alkaloids; Dyslipidemia; In Silico.; Pandan; Pandanus amaryllifolius

Alteration of IL-6, BMP-15 and GDF-9 Levels on PCOS Rat Models After Treated with Syzygium Polyanthum (Wight) Walp Leaves Extract - Renny Aditya; Budi Santoso; Widjiati (Pharmacognosy Journal, 2023)
Background: Polycystic ovary syndrome (PCOS) is a global health concern for women in reproductive age women. Numerous studies have been reported an association between chronic inflammation and alteration of cytokine in women with PCOS. Syzygium polyanthum (S. polyanthum) contains antioxidants and has antiinflammation activity. Objectives: This study aims to measure the alteration of IL-6, BMP-15, and GDF-9 in rat PCOS model after treated with S. polyanthum leaves extract. Materials and Methods: The female Wistar rats were divided into five groups (n = 5), K0 (normal control), K1 (PCOS group), and three treatment groups which received three different doses of S. polyanthum leaves extract. The treatment group consisted of PCOS rat models with S. polyanthum leaves extract supplementation of 150 mg/KgBW (P1), 300 mg/KgBW (P2), and 450 mg/KgBW (P3). Results: IL-6 expression was highest in K1 (4,690 ± 0.099) and lowest in the P3 treatment, namely (2,370 ± 0.105). The expression of BMP-15 and GDF-9 was lowest at K1 (2.554 ± 0.04; 4.502 ± 0.050) and highest at P3, namely (2.265 ± 0.072; 4.736±0.074). Conclusion: S. polyanthum leaves extract was significantly effective in decreasing IL-6 expressions, as well as a significant increase in BMP-15 and GDF-9 expressions in the PCOS rat model.
Keywords: BMP-15; GDF-9; IL-6; Syzygium polyanthum

Analysis Factors Affecting the Utilization of Antiretroviral Treatment Services in HIV Patients in Ambon City Puskesmas - Faradila Kilkoda; Balqis; Indar; Darmawansyah; Atjo Wahyu; Anwar Daud; Anwar Mallongi (Pharmacognosy Journal, 2023)
Acquired Immune Deficiency Syndrome (AIDS) is a collection of symptoms that arise due to decreased immunity caused by HIV infection. Human Immunodeficiency Virus (HIV) is a type of virus that infects white blood cells which causes a decrease in human immunity. Efforts are being made at this time for people with HIV namely Antiretroviral treatment (ARV). This study aims to analyze the factors that influence the utilization of ARV treatment services in HIV patients at the Ambon City Health Center. This type of research is a quantitative analytic study with a cross sectional design. Respondents in this study were obtained using the Isaac and Michael method of 156 HIV patients from the Waihaong Health Center and Karpan Health Center in Ambon City using a questionnaire. Data processing was carried out using SPSS and for data analysis using statistical tests using descriptive statistical tests, comparative analysis tests and multivariate analysis tests. The results showed that there was no effect of knowledge on the use of ARV treatment in HIV patients at the Ambon City Health Center. Furthermore, there is an influence on the patient's actions, access distance, and peer and community support on the use of ARV treatment in HIV patients at the Ambon City Health Center. The action variable and access distance are the variables that have the strongest influence compared to the other variables.
Keywords: AIDS; ARV; HIV; Puskesmas; Utilization

Analysis of Covid-19 Neutralizing Antibody Levels Post Vaccination using Ichroma™ and iFlash Covid-19 Nab - Hartono Kahar; Sari Prabandari Prasetyaningrum; Munawaroh Fitriah; Aryati; Jusak Nugraha (Pharmacognosy Journal, 2023)
Background: One of the efforts to control SARS-CoV-2 infection in health workers is vaccination. In this study, the levels of SARS-CoV-2 neutralizing antibody (nAb) in health workers were measured with Ichroma and iFlash. Methods: This study applied an observational analytic design with a prospective cohort and was conducted at Dr. Soetomo Regional Public Hospital, Surabaya, from January to November 2021. The population of this study included a total of 75 health workers after taking the second dose of the SARS-CoV-2 (Sinovac) vaccine. The Covid-19 NAb levels of the population were tested with Ichroma and iFlash on day 0 before vaccination, as well as days 14 and 28, and months 3 and 6 after vaccination. Results: The Friedman test indicated a significant difference in NAb levels according to the iFlash test on day 14, day 28, month 3, and month 6 compared to those before vaccination (p < 0.05). The Wilcoxon test revealed a significant difference in NAb levels on day 14, day 28, month 3, and month 6. The results of the Cochran test showed a significant difference in the positivity of NAb according to the Ichroma test on day 14, day 28, month 3, and month 6 compared to those before vaccination (p < 0.05). McNemar's test demonstrated that the COI at month 3 was not significantly different from that before vaccination; The COI at month 6 was not significantly different from those at days 14 and 28. The results of the Pearson correlation test and Bland–Altman plot indicated a moderate correlation between Ichroma and iFlash (r = 0.592, p = 0.002). Conclusion: Neutralizing antibodies for Covid-19 were formed after day 14 and started to increase on day 28 and started to decrease in months 3 and 6. The levels of NAb for Covid-19 were measured with Ichroma and iFlash in roughly the same pattern and had a moderate positive correlation.
Keywords: Ichroma; iFlash.; Neutralizing Antibody

Analysis of Deferred Blood Donor Candidates at Dr. Sardjito Hospital, Yogyakarta, Indonesia - Teguh Triyono; Tsaniatul Afifah; Usi Sukorini (Pharmacognosy Journal, 2023)
Background: The selection of blood donor candidates contributes to the safety of donors and donor recipients. An understanding of the characteristics, demographic characteristics, and types of potential blood donors are essential to obtain an accurate picture of the donor donors, thereby guiding recruitment strategies and providing benchmarks to assess the success of blood donation. Objective: This scientific work aims to analyze the deferred blood donor candidates annually based on the required blood donor characteristics at the Blood Transfusion Unit (BTU) of Dr. Sardjito Hospital, Yogyakarta, Indonesia, from 2017 to 2019. Method: This observational analytic study applied a retrospective design and was conducted at Dr. Sardjito Hospital, Yogyakarta. The data were collected from the donor registration information system and database in the blood request system for the 2017-2019 period. The statistical analysis was conducted using the Chi-Square test in the SPSS version 22. Results: The deferred blood donor candidates in 2017-2019 amounted to 618 (0.82%) out of 75,067 total blood donors, most of whom were female, reaching an annual percentage of 50.7% in 2017, 50.9% in 2018, and 59.1% in 2019. The age range of both male and female deferred blood donor candidates was 17-30 years. The Hb levels of < 12.5 g/dL were mostly found in female blood donors, reaching a peak of 82.4% in 2017, 83.9% in 2018, and 85.4.6% in 2019. Voluntary blood donors accounted for 94.7% (in 2017), 98.6% (in 2018), and 100% (in 2019) of the female deferred blood donor candidates. All analytical tests resulted in a p-value of < 0.0001. Most of the deferred blood donor candidates had blood type O with a p = 0.026. The most common cause of deferral was high or low Hb levels in female blood donors with an age range of 17-30 years. Conclusions: The deferred blood donor candidates in 2017-2019 amounted to 618 (0.82%) out of 75,067 total blood donors, most of whom were female with the most common deferral reason of Hb levels < 12.5 g/dL in the age range of 17-30 years.
Keywords: Donors rejected; Hemoglobin.; Transfusion

Analysis of Determinants of Midwife Performance in Antenatal Care Services in Allang Care Health Centers Central Maluku District - Josina Hattu; Darmawansyah; Amran Razak; Sukri Palutturi; Anwar Mallongi; Syamsiar S Russeng (Pharmacognosy Journal, 2023)
Antenatal care (ANC) is an important part of health care for pregnant women. Several studies conducted in high-income countries have shown that antenatal care can improve the care experience and health outcomes for pregnant women and newborns. This study aims to analyze the performance determinants of midwives in antenatal care services at the Allang Nursing Health Center, Central Maluku Regency. This type of research is a quantitative study with a cross sectional study approach. Respondents in this study were obtained by taking the entire population of 23 people using a questionnaire. Data processing was carried out using SPSS and for data analysis using statistical tests using univariate analysis tests, bivariate analysis tests and multivariate analysis tests. The results of this study indicate that there is a relationship between age, employee status and years of service to the performance of midwives in ANC services at the Allang Health Center, Central Maluku Regency and there is no relationship between knowledge and attitudes towards the performance of midwives in ANC services at Allang Health Center, Central Maluku Regency. Suggestions from this study are for the Office of Health and Midwives in Central Maluku District Provide policies in managing human resources for midwives with non-permanent employee status, with incentive arrangements that can improve performance in ANC services and carry out Antenatal care services with full sense of responsibility and seriously bearing in mind that the services provided are related to the scope of the Maternal and Child Health program to be achieved.
Keywords: Antenatal Care; Child health.; Health Center; Pregnant women

Analysis of Factors Affecting Exclusive Breast Milk in The Work Area of Waru Puskesmas East Seram Regency - Sukmawati Rumakur; Muhammad Alwy Arifin; Darmawansyah; Balqis; Furqaan Naiem; Suriah; Anwar Mallongi; Muh. Yusri Abadi (Pharmacognosy Journal, 2023)
At present exclusive breastfeeding is decreasing, the causes of the decline in exclusive breastfeeding are factors of lack of knowledge, social culture, awareness of the importance of breastfeeding, health services, health workers who have not fully supported, and the incessant promotion of exclusive breastfeeding. The low percentage of breastfeeding is probably due to the many factors that cause breastfeeding failure, both internal factors (mother's knowledge, mother's education, mother's occupation, and mother's disease) and external factors (promotional infant formula, birth attendants) which cause failure in breastfeeding for 6 months. This study aims to determine the factors that influence exclusive breastfeeding to infants in the work area of the Waru Health Center, East Seram District. This type of research is quantitative research with an analytic observational design with a cross-sectional study to see the effect of the independent variables on the dependent variable and observations at the same time. The sampling technique in this study was simple random sampling with a total sample of 81 mothers. The results of this study indicate that there is an influence between employment status and the support of health workers with exclusive breastfeeding, while for the variables of maternal pregnancy distance and knowledge, there is no effect with exclusive breastfeeding. Suggestions for health workers are to improve communication, information, and education. The advice for breastfeeding mothers is to further increase knowledge related to lactation and family support is needed.
Keywords: Exclusive breastfeeding; Infants; Puskesmas.

Analysis of Factors Affecting the Behavior of Exclusive Breastfeeding in the Work Area of Werwaru Puskesmas Moa Lakor District - Magdalena Akse; Indar; Amran Razak; Balqis; Wahiduddin; Syamsuar Manyullei; Anwar Mallongi (Pharmacognosy Journal, 2023)
Exclusive breastfeeding is not only the best food for babies, but is important for the health of breastfeeding mothers and provides optimal growth for babies. Exclusive breastfeeding for babies aged 0 to 6 months and continued until the child is 2 years old can encourage healthy growth and development. This study aims to analyze the factors that influence exclusive breastfeeding behavior in the working area of the Werwaru Health Center, Moa Lakor District. This type of research is a quantitative analytic study with a cross sectional design. Respondents in this study were obtained using the proportional sampling method as many as 73 toddlers aged 6-24 months from the Werwaru Health Center, Moa Lakor District, which consisted of 6 villages (Werwaru, Klis, Pakty, Upyour, Syota, and Comfort) using a questionnaire and measuring instruments. anthropometry. Data processing was carried out using SPSS and for data analysis using statistical tests using univariate, bivariate and multivariate analysis tests. The results showed that internal factors that had an effect partially were knowledge while simultaneously namely perceptions of exclusive breastfeeding behavior in the Working Area of the Werwaru Health Center, Moa Lakor District. Meanwhile, external factors that have a partial effect are work and those that have a simultaneous effect are the support of the closest people to the behavior of exclusive breastfeeding in the Working Area of the Werwaru Health Center, Moa Lakor District. Suggestions for the government of Southwest Maluku Regency through the Werwaru Health Center, Moa Lakor District, to be able to improve the exclusive breastfeeding program by disseminating information on the benefits of exclusive breastfeeding in the morning for pregnant and lactating women so that mothers' knowledge increases.
Keywords: Exclusive breastfeeding; Health centers.; Toddlers

Analysis of Knowledge and Skills of Cadres in Early Detection of Stunting Toddler Development in Soppeng Regency, Indonesia - Hasriani; Andi Ummu Salmah; Masni; Apik Indarty Moedjiono; Healthy Hidayanty; Wahiduddin; Anwar Mallongi (Pharmacognosy Journal, 2023)
Background: About 16% of Indonesian toddlers experience neuro developmental and brain disorders ranging from mild to severe, every two days 1,000 babies experience motor development disorders. The prevalence of stunting in South Sulawesi is in the 20th lowest ranking, namely 27.4% based on the provincial level in 2021. Objective: Recognizing the increased knowledge and skill of cadre and mothers and toddlers in early development detection of stunted infants using a Developmental Pre-Screening Questionnaire (DPQ) in the working area of the Panincong Health Center, Soppeng Regency. Method: This study used a quasi-experimental design with a pretest-posttest control group involving 30 cadres and 30 mothers with 15 children in each group selected by systematic random sampling. Data were analyzed using paired t-tests and Independent t-tests. Results: The results showed that there were differences in knowledge (p=0.000) in the intervention group and differences in knowledge levels in the control group (p=0.044). The level of knowledge after treatment was greater in the intervention group (11.8%), while the knowledge after treatment in the control group was only (2.9%). There was a difference in the skills of the cadres (p=0.000) after being given the Android-based DPQ intervention. There was a difference in the knowledge (p=0.000) and skills of toddler mothers (p=0.000) after being given the Android-based DPQ intervention applied by the cadres. Conclusion: Android-based DPQ is effective as an educational medium and as an alternative to cadres and parents in monitoring or early detection of child development using Android-based DPQ.
Keywords: Cadre; Development Disorders; Questionnaire.; Stunting; Survey

An Analysis of Local Knowledge of Phit-Samdaeng in Yang Sisurat District, Maha Sarakham Province and The Medicinal Plants Used to Relieve Its Symptoms - Hatthapan Wipanso; Bhanubong Bongcheewin; Suppachai Tiyaworanant; Suksalin Booranasubkajorn; Natchagorn Lumlerdkij; Pravit Akarasereenont (Pharmacognosy Journal, 2023)
Introduction: “Phit-Samdaeng” is a Thai term often used to refer to an illness of eating unsuitable foods. However, its use in a diagnosis is limited due to the unclear definition and description. This study aimed to compare knowledge of Phit-Samdaeng symptoms and treatments in Northeastern folk medicine, Thai Traditional Medicine, and medicinal plants in the Yang Sisurat District of Thailand. Methods: Semistructured interviews and participatory observations were used to collect data from 12 folk healers. Questions were designed to obtain local knowledge of Phit-Samdaeng and medicinal plants. The data was analyzed using descriptive statistics, comparison and interpretation. Results: Some symptoms of Phit-Samdaeng in the nine scriptures of Thai Traditional Medicine, Tumraya Silajaruek Watprachethupon, and of Kin-Phit and Phit-Kaboon, in Tamraya Boran Isan, were similar in Yang Sisurat. However, the names were different. There were also differences in species and the recipes of herbal remedies among folk healers and in the textbooks. The most used plants to treat Phit-Samdaeng by folk healers were Clerodendrum paniculatum L. and Clausena wallichii Oliv. var. guillauminii (Tanaka) Molino. Only nine species had pharmacological effects relating to Phit-Samdaeng treatment. Conclusions: The knowledge of Phit-Samdaeng symptoms in Yang Sisurat District were consistent with the Northeast folk medicine and Thai Traditional Medicine. The most commonly used species were native plants of Thailand. However, information on the medicinal taste and property from scriptures and pharmacological evidence is lacking. Therefore, the pharmacological activity and toxicity of herbal remedies should be further investigated for efficacy and safety.
Keywords: Alternative medicine; Folk knowledge; Herbal medicine; Phit-Samdaeng.

Analysis of Lung TB Treatment Strategy in Pangkep District Year 2022 - Muhammad Syafar; Ridwan Mochtar Thaha; St. Rosmanely; Rizky Chaeraty Syam; A. Tenri Nurafni Aprilyani (Pharmacognosy Journal, 2023)
Tuberculosis (TB) is still a world concern with morbidity rates that have not been fully recorded but mortality data continues to increase, so strengthening coordination strategies with various related networks is very important in the target of achieving TB eradication nationally and globally in 2035. This research is a qualitative research carried out to identify phenomena and problems related to pulmonary TB management strategies in Pangkep Regency in October 2022. Data were collected through Focus Group Discussions (FGD), which were then analyzed qualitatively through 3 stages, namely data reduction, data presentation, and data verification as well as drawing conclusions. The results of the study show several important points in the strategy for handling pulmonary TB in Pangkep Regency, namely the applicable policies still follow the policies from the center, access for island areas must have a special strategy, the TB control strategy used is TOSS and there is collaboration between cross-sectors, electronic recording is carried out through SITB and manuals, as well as monitoring and evaluation activities carried out by looking through the system, conducting routine monitoring and evaluation, as well as field visits.
Keywords: Policy; Pulmonary Tuberculosis.; Treatment strategy

The Analysis of Matrix Metalloproteinase-9 and Tissue Inhibitor Matrix Metalloproteinase-1 Levels in the Amniochorion Membrane Patients on Premature Rupture of Membranes - Meidrin Joni; Eryati Darwin; Herlambang Herlambang; Aisyah Elliyanti (Pharmacognosy Journal, 2023)
Introduction: MMP-9 is essential for extracellular matrix remodeling, which affects the incidence of premature rupture of membranes. In addition, decreased and increased levels of TIMP-1, a preferential MMP-9 inhibitor, have been reported to be associated with premature rupture of membranes because it showed an imbalance in the MMP-9 or TIMP-1 levels. This study aims to analyze MMP-9 and TIMP-1 levels in amniochorion membrane patients on premature rupture of membranes. Methods: An analytic observational study was conducted on 70 subjects. The MMP-9 and TIMP-1 levels in the amniochorion membrane were determined by ELISA. Results: The results of this study indicate that the amniochorion membrane in the incidence of premature rupture of membranes is characterized by increased levels of MMP-9, while TIMP-1 levels do not differ between the incidence of premature rupture of membranes. Conclusion: Based on the research that has been done, it can be concluded that there are differences in protein levels of MMP-9 but there are no differences in protein levels of TIMP-1 in premature rupture of membranes
Keywords: MMP-9; Premature rupture of membranes.; TIMP-1

Analysis of Performance Determinants of Health Laboratory Officers in Health Laboratory Centers and Medical Device Calibration in Maluku Province - Herlyn De Fretes; Darmawansyah; Muhammad Alwy Arifin; Amran Razak; Lalu Muhammad Saleh; Ummu Salmah; Anwar Mallongi (Pharmacognosy Journal, 2023)
The Center for Health Laboratories and Medical Devices Calibration has the task of planning, coordinating implementation and evaluation of clinical laboratory examinations/public health laboratories, referrals, education, technical training, research and development. Performance is the result achieved by someone according to the standards that apply to a particular job. There are several factors that influence the performance of officers, both individually and in organizations, namely the dimensions of performance, namely ability, motivation and opportunities. This study aims to analyze the factors that influence the performance of the staff at the Maluku Provincial Health Laboratory and Medical Device Calibration Center based on factors related to work, related to opportunities, related to infrastructure and related to officers. This type of research is a quantitative research with a survey method to obtain data from the field based on the existing population with a total sample of 56 people. Data processing is done using SPSS. The results showed that the opportunity factor and staff factor had a significant influence on the performance of the staff at the Maluku Province Health Laboratory and Medical Equipment Calibration Center, while the factors that did not give an effect were infrastructure and work factors. Suggestions for related agencies, namely the Health Service must provide motivation, work autonomy, workload according to ability, carry out work effectively and efficiently, communicate regarding access and availability of medical devices and must be able to strengthen the activeness of officers in carrying out tasks with supervision that does not interfere with autonomy health worker job.
Keywords: Equipment; Laboratory Hall; Laboratory Officer; Medical devices.; Performance

Analysis of Policy Implementation of Minimum Service Standards for Hypertension in Marusu Health Center Maros District - Wafika Aziza Nasir; Sukri Palutturi; Alwy Arifin; Balqis; Wahiduddin; Anwar Daud; Anwar Mallongi (Pharmacognosy Journal, 2023)
The Minimum Service Standards (SPM) in the health sector are a reference for Regency/City. Regional Governments in the provision of Health Services that every citizen is entitled to receive at a minimum. The MSS for Hypertension health services is one of the types and quality of basic services in the MSS in the health sector. This study aims to analyze the implementation of the MSS for hypertension services at the Marusu Health Center, Maros Regency. This type of research uses descriptive qualitative which aims to get an in-depth description of the analysis of the implementation of the minimum service standard for hypertension at the Marusu Health Center. The results of the study show that communication has not run optimally, the SPM Hypertension policy contained in PMK No. 4 of 2019 has not been transmitted to the public as a policy target. Human resources are still inadequate and have a double workload so that the achievements are not optimal. Disposition has been going pretty well. commitment and implementation support are quite good, has carried out coordination and commitment involving cross-programs and related cross-sectors. The bureaucratic structure is already running quite well. The implementation mechanism has been running according to the SOP with the guidelines of the Minister of Health Regulation No. 4 of 2019. The implementation of the SPM for hypertension services at the Marusu Health Center has been going quite well. It is hoped that the Maros District Health Office and the Marusu Health Center will improve the aspects of communication, resources, disposition and bureaucratic structure in their implementation.
Keywords: Commitment.; Hypertension; Minimum services; Policy implementation

Analysis of Policy Implementation of The First 1000 Days of Life Program in Overcoming Stunting in Maros District - Andi Sri Fifi Wahyuni Azis; Darmawansyah; Amran Razak; Alwy Arifin; Muh Syafar; Anwar Mallongi (Pharmacognosy Journal, 2023)
Stunting is closely related to the first 1000 Days of Life. This 1000 HPK period starts from pregnancy until the age of 2 years. The aim of the study was to analyze the implementation of the First 1000 Days of Life program policy in Stunting Management in Maros Regency. This study uses a qualitative panel research method with a descriptive approach. Data were analyzed using content analysis. Interviews were conducted with 9 informants in several agencies, including 3 key informants and 6 regular informants. Research result: Communication between Organizations and Executing Activities (The process of conveying information about the 1000 HPK program in tackling stunting is carried out in the form of good socialization). Resources (Almost fulfilled in carrying out the 1000 HPK program but the capacity of human resources is still inadequate in this case the understanding and availability of budgets sourced from APBD funds and APBN funds). Characteristics of the Executing Agency (There is an SOP for the implementation of 1000 HPK. In the distribution of tasks and responsibilities to all policy implementers of the 1000 HPK program contained in the SK so that each cross-sector coordinate with each other. Disposition of Executors (Executors have played an active role and have a commitment for the success of the 1000 HPK program). Conclusion: Socialization activities have not gone well; several agencies are still experiencing a shortage of human resources both in quantity and quality and experiencing budget shortages resulting in inadequate facilities. In implementing the 1000 HPK program policies, several agencies used the SOP as a procedure for implementing the program as well as the division of tasks and responsibilities based on the Decree of the Maros Regent. Special suggestions for the health office to pay more attention to and have a high commitment to the nutritional status of children by ensuring specific nutrition interventions and sensitive nutrition interventions are right at the location of the village and at the right target group.
Keywords: First 1000 Days of Life; Policy; Stunting.

Analysis of Policy Recommendations in Efforts to Control Positive COVID-19 Cases in South Sulawesi Province, Indonesia - Sri Nuriana; Balqis; Sukri Palutturi; Indar; Yahya Thamrin; Stang; Anwar Mallongi (Pharmacognosy Journal, 2023)
Introduction: Inconsistent COVID-19 policies have caused the government to experience a degradation in public trust. So, it is necessary to propose policy recommendations for controlling positive COVID-19 cases that receive support from various multi-sectoral areas. Method: This research was conducted in January–May 2023. This type of research is qualitative-exploratory, using case study methods through indepth interviews and literature studies of all informants. Sampling was purposive sampling. This research variable uses Patton and Sawicki's alternative policy criteria theory (1986). Results: The proposed policy recommendations are increasing COVID-19 vaccine coverage and surveillance for monitoring coronavirus mutations. The two policy recommendations have met technical feasibility because they have clear outcomes, where the outcome of vaccination is to establish herd immunity. In contrast, surveillance of coronavirus mutations has the outcome of analyzing the efficacy of the COVID-19 vaccine. From the economic and financial possibility side, a budget is available with the advantage of carrying out normal activities without any restrictions and efficiency in the health budget. Regarding political viability, the government supports the policy, does not violate the rules, and serves the community's needs. Regarding administrative operability, the government as the authorized party is considered to have adequate capability, organizational support, and institutional commitment. Conclusion: The policy recommendations proposed by policymakers and epidemiology experts are increasing COVID-19 vaccination coverage and monitoring surveillance of coronavirus mutations, and it is hoped that the South Sulawesi Provincial Government can consider the proposed policy recommendations.
Keywords: COVID-19; Health Policy; Policy Analysis; Surveillance; Vaccination

Analysis of Royal Jelly Apis Cerana as Therapeutic Candidate in Cbavd Based on Bioinformatics Studies - Maslichah Mafruchati; Jonathan Makuwia (Pharmacognosy Journal, 2023)
Background: Apis cerana honey bee is a honey bee native to Asia that spreads from Afghanistan, China, and Japan to Indonesia. The productivity of Apis cerana honey bees can produce as much as 2-5 kg of honey per colony in a year. Royal jelly is the queen's nourishment, and it is produced by larvae, adult bees, young worker bees, and male bee larvae. CBAVD contributes to 1–2% of the 20–25% of males with subfertility. Aim: The objective of this study was to evaluate Royal Jelly Apis Cerana as a potential therapeutic candidate for CBAVD using a bioinformatics approach. Method: This research method consisted of converting nucleotides into amino acids, analyzing the three-dimensional structure of Apis Cerana Royal Jelly Protein, Ramachandran Plot Analysis, Analysis of Epitope and Allergen Proteins, and Analysis of Proteins that were antigens and toxins. Results: The research results were conducted on six three-dimensional Apis Cerana Royal Jelly bee protein structures and had very good validity based on the Ramachandran plot, GQME value, and QmeanDisCo value. In addition, this study also obtained the results of proteins that are epitope, antigenic, non-allergenic, and non-toxic. Conclusion: The findings of this study can be used as a basis for therapy against CBAVD. <quillbot-extension-portal></quillbot-extension-portal>
Keywords: Apis Cerana; Bioinformatics; CBAVD; Public health.; Therapeutic Candidates

Analysis of The Effect of Leptin, AMPK, Adiponectin, and NPY Markers on Changes in Body Weight of Childhood Epileptic Using Valproic Acid Monotherapy - Wardah Rahmatul Islamiyah; Nasronudin; Abdulloh Machin; Iin Ernawati; Yunita Dwi Tanti; Nur Jaya; Farah Medina; Fathia Faza Rahmadanita; Paulus Sugianto (Pharmacognosy Journal, 2023)
Introduction: Epilepsy is a neurological disorder that occurs due to abnormal neurons in the brain and an imbalance between excitation and inhibition in the central nervous system. The first line of OAE in children is VPA (Valproate Acid). However, long-term use can cause weight gain with a frequency of 10-70%. The underlying mechanism of weight gain in patients remains unclear. Purpose: This study aimed to analyze the relationship between levels of biomarkers AMPK, NPY, Leptin, and Adiponectin on changes in body weight in patients with epileptic seizures using VPA monotherapy. Method: This study is an observational cohort design. Data collection in April-June 2019. Inclusion criteria were children aged 2-10 years who used VPA requirement less than two years, not taking any drugs that affect body weight, not diagnose systemic lupus, nephrotic syndrome, and diabetes mellitus. Bodyweight and all biomarkers measurement on subjects who came to the clinic at the time of study and at least after one month of taking VPA. A total of 17 subjects participated in this study. Result: The results of the statistical multivariate analysis test of VPA dosage on changes in body weight and biomarker levels found that Leptin, AMPK, Adiponectin did not significantly increase in body weight (p>0.05), but NPY significant increase in body weight (p<0.05). Conclusion: NPY is the most potent for appetite enhancing, preferential effect on carbohydrate intake, weight regulation, energy storage, and expenditure. Increase production of NPY, there is an increase in energy intake and then increases fat storage and body weight.
Keywords: Adiponectin; AMPK; Childhood.; Epilepsy; Leptin; NPY; Valproic acid; Weight gain

Analysis of the Impact of Heat Stress on Embryo Development of Broiler: A Literature Review - Maslichah Mafruchati; Nor Hayati Othman; Akhmad Kusuma Wardhana (Pharmacognosy Journal, 2023)
Temperature is an important factor in embryonic development because temperature plays a role in determining the overall development of the embryo. The effect of high temperature on the embryo will cause the embryo to make a defense by diverting some of its energy to form thermal homeostasis, which is a physiological response. The purpose of this study was to analyze heat stress on embryonic development. This study used a systematic literature review (SLR) following PRISMA guidelines and gathered the sample of papers through the PubMed databases using keywords categorized as heat, stress, and embryo. There were 31 papers used as samples. The result showed that hyperthermia in chickens caused an increase in heart rate per minute as a result of temperature pressure. It affects the hatching percentage, hatching time, weight, and mortality rate in chicken embryos. In summary, heat stress has an impact on embryonic development heat stress gives an impact on the development of embryos.
Keywords: Chicken; Embryo Development; Food security; Health risk; Heat Stress.

Analysis of the Influence of Internal and External Factors on Empowered Pregnant Women Through the Behavior of Pregnant Women in Early Detection of Pregnancy Complications - Bintang Petralina; Ridwan Amiruddin; Wahiduddin; Irwandy; Anwar Mallongi; Ummu Salmah; Suriah; Evi Martha (Pharmacognosy Journal, 2023)
Objective: Maternal health is still one of the health problems globally, including in Indonesia. Empowering women that allows someone in making decisions about themselves to detect pregnancy complications. This study aims to analyze the influence of internal and external factors on empowered pregnant women through the behavior of pregnant women in conducting early detection of pregnancy complications [KOMPAK] in Bogor Regency. Method: Village in study is an analytical survey with a cross sectional study approach, which takes data related to internal factors (age, education, and parity) and external factors (husband support, family support, community support, and health worker support), behavior (knowledge, attitudes and motivation), and empowered pregnant women. It was analyzed using the spearman correlation test and path analysis with a confidence level of 95%. The data was analyzed using the SPSS program version 26.0. Results: internal factors that influence empowered pregnant women in Conducting Early Detection of Pregnancy Complications [KOMPAK] in Bogor Regency are education (r: 0.194; p<0.001) and parity (r: -0.108; p: 0.044) which means that the higher the education and the less parity, the better empowered pregnant women will be with weak correlation strength. External factors affect Empowered Pregnant Women in Early Detection of Pregnancy Complications [KOMPAK] in Bogor Regency where external factors (husband support, family support, culture and community support, and health worker and government support) will cause empowered pregnant women to be better with moderate correlation strength (r: 0.537-0.645; p< 0.001). Conclusion: Internal factors (education and parity) and external factors (husband support, family support, culture and community support, and health worker and government support) affect (empowered pregnant women in early detection of pregnancy complications. External factors also influence pregnant women through maternal behavior (knowledge, attitudes, and motivations).
Keywords: Behavior; Empowered pregnant women; External factors; Internal factors

Analysis of The Influence of The Marketing Mix on The Satisfaction of Patients with Type 2 Diabetes Miletus Installation at Hajj General Hospital, Makassar - Suci Safwa Salsabila; Indar; Darmawansyah; Amran Razak; Syamsuar Manyullei; Lalu Muhammad Saleh; Anwar Mallongi (Pharmacognosy Journal, 2023)
Marketing mix is a set of marketing tools used by companies to achieve marketing objectives. The marketing mix consists of nine components, namely product, price, place, promotion, people, process, physical evidence, performance, and strength. This study aims to determine the effect of the marketing mix on patient satisfaction with type 2 diabetes mellitus inpatient installations in public hospital in the Hajj area of Makassar, the type of research used was an analytical survey with a quantitative approach using cross sectional. The sample in this study was type 2 diabetes mellitus patients in the inpatient installation at the Hajj area general hospital as many as 81 people who were obtained by the probability sampling method, collecting data using a questionnaire instrument. Processing data using the Chi-Square test and multivariate logistic regression analysis. Based on the results of the study, there is an influence between the process (Process). Promotion, Place, and Power on patient satisfaction with type 2 diabetes mellitus in inpatient installations. The results of the multivariate analysis showed that the most dominant variable was placed (exp β = 19,093). The conclusion of several influential variables that are most related to patient satisfaction with the marketing mix, namely the place variable, which is the most important factor in patient satisfaction. It is hoped that the Haji Makassar Hospital will pay more attention to the services needed by the community in general, especially the location in terms of the number of rooms, parking and waiting rooms that are more adequate.
Keywords: Place; Power; Price; Process; Product; Promotion; Satisfaction.

Analysis of the Metabolite Compound of the Ethanol Extract of Chrysanthemum cinerariifolium Stem and Activity for inhibition of Oral Squamous Cell Carcinoma (OSCC) in silico study - Anik Listiyana; Yuanita Lely Rachmawati; Hani Susianti; Nurdiana; Hidayat Sujuti; Roihatul Mutiah; Agustina Tri Endharti (Pharmacognosy Journal, 2023)
Background: Oral cancer is a deadly disease that is expected to increase yearly. Current cancer treatment methods have side effects. C. cinerariifolium plants have potential as anticancer agents. Objective: To evaluate the anti-OSCC properties of the ethanol extract of C. cinerariifolium stems via an in-silico study. Materials and Methods: Analysis of active compounds in ethanol extracts of C. cinerariifolium stems using TLC and UPLC-QToF-MS/MS metabolic profiling. The data were analysed statistically using principal component analysis (PCA). In silico of C. cinerariifolium compounds on protein (PI3K and Cyclin D) from OSCC. Results: TLC procedures utilizing UV light with λ 366 nm after spraying with H2SO4 revealed multiple-colored spots, indicating that H2SO4 is a specific spray detector for terpenoid and carotene. Metabolic profiling in ethanol extract of C. cinerariifolium stem included Pronethalol (3.96%), 1-(4-Methoxyphenyl)-N-(1 naphthylmethyl) methanamine (7.34%), Orphenadrine (24.27%), Pentazocine (5.09%), 4-(Dodecyloxy) aniline (6.30%), Linoleamide (4.95%), and Pheophorbide A (8.05%). Orphenadrine had the highest percentage. Based on the Lipinski rule of five, pronethalol has the potential to be used as a drug-like therapy for OSCC. The anticancer activity profile is predicted by PASS online with a likely range of 0.065 to 0.385. An in-silico study showed that the strongest binding affinity is pronethalol to Cyclin D1 and pheophorbide A to the PI3K protein. Conclusion: The active metabolite of the ethanolic extract of C. cinerariifolium stem exhibits potency against oral squamous cell carcinoma via the downregulation of the cell cycle (cyclin D1) and P13K, especially pronethalol.
Keywords: Chrysanthemum cinerariifolium; Cyclin D1; OSCC; P13K.

Analysis of the Phytochemical and Antibacterial Properties of the Indigenous Mizo Medicinal Plant, Helicia Excelsa - Lalbiakngheti Tlau; Lucy Lalawmpuii; P.B. Lalthanpuii; K. Lalchhandama (Pharmacognosy Journal, 2023)
Background: Helicia excelsa (Roxb.) Bl. is a scarcely known medicinal plant and is native to Southeast Asia. It is most notably used for the treatment of gastric problems in the Mizo traditional medicine in India. Method: The leaves of H. excelsa were collected from Aizawl, Mizoram, India. An extract was prepared using chloroform. Qualitative phytochemical tests were performed to detect the important phytocompounds. The antioxidant activity was determined by total phenolic content, total flavonoid content, total antioxidant content, DPPH- and ferric-reducing antioxidant power. Antibacterial activity was evaluated by agar well-diffusion method. Results: H. excelsa leaf contains amino acids, alkaloids, carbohydrates, glycosides, phenols, phytosterols, proteins, and tannins. It showed inhibition in selected Gram-negative and Gram-positive bacteria. The phenol, flavonoid and total antioxidant contents were 4.52±0.09 gallic acid equivalent (GAE mg/g), 64.27±1.04 quercetin equivalent (QE mg/g), 11.39±0.45 ascorbic acid equivalent (AAE mg/g) respectively. IC50 value of DPPH-scavenging activity was 5.67±0.36. The ferric ion-reducing power showed concentration-dependent activity. The plant extract showed growth-inhibitory actions against Gram-negative bacterium, Escherichia coli, and Gram-positive species, Bacillus cereus and Staphylococcus aureus. :Conclusion H. excelsa leaf contains important bioactive compounds that need to be identified. The antioxidant and antibacterial activities support the basis of its medicinal application.
Keywords: Antibacterial activity; Antioxidant; Helicia excelsa; Mizo traditional medicine.; Plant extract

Another Mode of Action of Temephos Against Aedes aegypti Larvae: A Stomach Poison Investigation - Hebert Adrianto; Sri Subekti; Heny Arwati; Etha Rambung; Hanna Tabita Hasianna Silitonga; Etik Ainun Rohmah (Pharmacognosy Journal, 2023)
Introduction: Aedes aegypti is a key vector for the spread of several severe arboviral infections. The Indonesian Ministry of Health launched Temephos as a national effort to limit the Aedes aegypti larvae population. The old theory has been passed down for generations that the principle of the mechanism of action of temephos as a neurotoxin. The main aim of this study was to investigate the mechanism of action of temephos as a stomach poison by using histopathology study. Method: There are two treatments with three replications: a container containing only 100 ml of water with tween 20 and a container containing 100 ml of water with 1 ppm of temephos 8G. The 20 third-instar Ae. aegypti larvae in containers containing 100 ml of water with 1 ppm of temephos 8G were compared with those in 100 ml of water containing Tween-20. The experiment was done in three replications. The number of dead larvae was recorded after 24 hours of treatment. Histological sections of the larval midgut were prepared and stained with hematoxylin-eosin (HE). Light microscopy was used to examine changes in the length of the midgut lumen and the epithelium. Data were analyzed using a one-way ANOVA. The appearances of the nucleus of the epithelial cell and the degree of damage were qualitatively observed. Results: The results showed that no dead larvae were found in the control group, however, 100% mortality was found in the temephos group. The changes in midgut lumen length and in the epithelium length were significantly different from those in the control group (p<0.05). Nuclei of epithelial cells were lost and midgut cells were damaged in the temephos group. Conclusions: This study reports the first discovery of the mechanism of action of temephos other than a neurotoxin, namely stomach poison
Keywords: Ae. aegypti; Histopathology; Midgut; Temephos

Antibacterial and Anti-quorum Sensing Activities of Erianthemum dregei`s Leaf Extract and Molecular Docking - Tsolanku Sidney Maliehe; Tlou Nelson Selepe; Nokuthula Nomusa Mthembu; Jabulani Siyabonga Shandu (Pharmacognosy Journal, 2023)
Background: The increasing incidence of multi-drug resistance among pathogens has propelled researchers to search for novel antimicrobial and anti-quorum sensing agents characterised by different mechanisms and high potency. Objective: The study aimed at investigating the antibacterial and antiquorum sensing properties of compounds from Erianthemum dregei and their molecular interactions with the target proteins. Methods: The methanolic leaf extract from E. dregei was evaluated for its chemical composition and antibacterial activity using gas chromatography-mass spectrophotometry (GC-MS) and micro-dilution method, respectively. The inhibition of violacein production in Chromobacterium violaceum (ATCC 07) was assayed as anti-quorum sensing activity using micro-dilution method. The molecular docking of the GC-MS ligands and penicillin‑binding protein 2x (PDP2) and CviR was executed using AutoDock Vina. Results: The two volatile compounds namely phytol (93.58%) and 3-tetradecyn-1-ol (6.42%) were shown by GC-MS. The extract exhibited antibacterial activity against the selected bacterial strains with minimum inhibitory concentration (MIC) values ranging from 1.56 to 3.125 mg/mL. The maximum inhibition of violacein production of 53.93% was observed at 1.56 mg/mL. Both compounds had docking scores of more than -6.0 kcal/mol against the target proteins. Conclusion: The results revealed that the extract is a potential source of antibacterial and anti-quorum sensing compounds and thus can have pharmacological applicability.
Keywords: Anti-quorum sensing activity; Antibacterial activity; Erianthemum dregei; Molecular docking

Anticancer and Neuroprotective Effects of the Triterpene Glycosides From Sea Cucumber Holothuria imitans - Marilú Roxana Soto-Vásquez; Paul Alan Arkin Alvarado-García; Demetrio Rafael Jara-Aguilar; Elda Maritza Rodrigo-Villanueva; José Gilberto Gavidia-Valencia; Iris Melina Alfaro-Beltrán; Bertha Mirella Alfaro-Ttito (Pharmacognosy Journal, 2023)
Introduction: Sea cucumbers has gained notoriety because possess a wide range of biological and pharmacological activities. In this sense, the aim of this work was to evaluate the anticancer and neuroprotective effects of the triterpene glycosides from sea cucumber Holothuria imitans. Methods: Triterpene glycosides were separated and purified by Reversed-phase high-performance liquid chromatography (RP-HPLC). Their structures were deduced by spectral analysis and chemical evidence. Cytotoxic activity was evaluated using normal African green monkey kidney epithelial cell line (VERO) and three cancer cell lines: cancer gastric (MKN-28), breast adenocarcinoma (MCF-7) and lung carcinoma (A-549). Besides, the neuroprotective effect was studied using the Cath.a-differentiated (CAD) cell line and human glial (Oligodendrocytic) hybrid cell line (MO3.13). Results: Two triterpene glycosides (Fuscocineroside C and Scabraside D) were isolated, which showed low cytotoxic activity against VERO cell line, and high cytotoxic activity against lines MKN-28, MCF-7 and A-549 cells, with IC50 between the ranges of 0.92 μmol/L to 2.61 μmol/L. The isolated triterpene glycosides showed the ability to regain mitochondrial viability in CAD and MO3.13 cells treated with neurotoxin (C2-ceramide) with statistically significant results (p<0.05). Conclusion: The triterpene glycosides Fuscocineroside C and Scabraside D isolated from sea cucumber Holothuria imitans show anticancer and neuroprotective potential and may be considered promising active principles for anticancer and neuroprotective drugs.
Keywords: Anticancer; Holothuria imitans; Neuroprotective.; Triterpene glycosides

The Antidepressant Effect of Fluoxetine and Mozart K448 Combination Therapy on Hippocampal Serotonin and BDNF Levels - Lisa Pangemanan; Irwanto Irwanto; Margarita M. Maramis (Pharmacognosy Journal, 2023)
Despite the use of fluoxetine as a first-line therapy, some patients do not show a good therapeutic effect. Effective antidepressant therapy will reverse the low serotonin and BDNF levels found in depression. Mozart K. 448 was reported to yield a good therapeutic effect for depression. Based on findings, the combination of Mozart K. 448 and fluoxetine as a therapy for depression is very rare. Therefore, this study aimed to determine the effect of the combined therapy of fluoxetine and Mozart K. 448 on hippocampal serotonin and BDNF levels in an animal model of depression under CUMS conditions. In this study, the animal model of depression was administered three different treatments, i.e. fluoxetine, Mozart, or fluoxetine-Mozart combined therapy, respectively. Hippocampal serotonin and BDNF levels were assessed after 21 days of treatment. Statistical analysis was then carried out using T-test or Mann-Whitney test and ANOVA or Kruskal-Wallis. The fluoxetine-Mozart group has higher BDNF levels, but lower serotonin levels compared to other groups with values of 1,694±0.215 and 44,533±3,275, respectively.
Keywords: BDNF; Fluoxetine; Hippocampus; Mozart; Serotonin

Antidiabetic and Hypoglycaemic Activities of Commonly Used African Traditional Vegetables - Nolitha Nkobole; Lavhelesani R. Managa; Gerhard Prinsloo (Pharmacognosy Journal, 2023)
Introduction: Diabetes mellitus is a common and complex metabolic disorder associated with high blood glucose levels leading to complications. Adopting a sedentary lifestyle characterized by low physical activity and consumption of high-energy diets contributes to the development of diabetes mellitus. Lifestyle changes and the use of pharmacological agents that target particular biochemical pathways involved in nutrient metabolism are currently used as management guidelines for managing risk factors associated with diabetes mellitus. The use of prescription medications for an extended period is linked to several negative side effects. Alternative management strategies of risk factors linked to diabetes mellitus involve the use of African leafy vegetables. African leafy vegetables contain a variety of biologically active compounds that provide health benefits. These crops have the potential to be a valuable source of new oral hypoglycemic agents for diabetes management. This review analyses the antidiabetic activities of nine African leafy vegetables whilst also defining the gap areas for future research. Methods: Data was acquired via electronic search engines of which only peer-reviewed papers published in journals were considered. Results: African traditional vegetables showed diverse in vitro and in vivo antidiabetic activities. Conclusions: There is an urgent need to document and use the knowledge of African leafy vegetables that have potential in the treatment and management of diabetes mellitus.
Keywords: African traditional vegetables; Antidiabetic; Diabetes mellitus; Hypoglycaemic; Phytochemicals.

Anti-Gastritis Activity of Cloves (Eugenia caryophyllata Thunberg) and Lime (Citrus aurantifolia) Leaf Extracts Combination in Absolute Alcohol Induced-Gastric Injury Mice - Herra Studiawan; Sukardiman; Indana Lazulfa; Rosita Handayani (Pharmacognosy Journal, 2023)
Background: Clove (Eugenia caryophyllata Thunberg) and lime (Citrus aurantifolia) are medicinal plants traditionally used to treat various diseases such as gastritis. Some studies reported that cloves leaf and lime leaf extract showed a gastroprotective effect by decreasing the gastric acid secretion and increasing the gastric mucus. Aim: This study aims to investigate the anti-gastritis activity of the combination of ethanolic extract of cloves leaf and lime leaf extract in alcohol absolute-induced mice. Methods: Mice were treated with the combination extract 0.7 g/kg BW, 1.4 g/kg BW, and 2.8 g/kg BW. Famotidine was used as a positive control, and Na CMC suspension was used as a negative control. After 45 minutes of oral administration, 0.14 ml/20g BW alcohol absolute was given to all mice. All groups were sacrificed one hour later. The evaluation showed that extract combination in all doses significantly decreased ulcer index (UI) compared to a negative control group (p<0.005) in macroscopic evaluation. Results: In histopathologic evaluation, all doses significantly decreased mucosal edema and epithelial cell loss (p<0.005), but in gastric bleeding evaluation, only 0.7 and 1.4 g/kg BW doses showed a significant decrease. Conclusion: This study showed that a combination of clove and lime leaf extracts has anti-gastritis activity and could be a possible therapeutic of anti-gastritis. The most effective dose is 1.4 g/kg BW in mice which showed the lowest ulcer index and gastric mucosal edema, bleeding and epithelial cells loss reduction on histopathological observation.
Keywords: Citrus aurantifolia; Clove; Eugenia caryophyllata; Gastritis; Lime; Ulcer

Antihypertensive Choices during Pregnancy in Limited Setting - Ernawati; Aditiawarman; Salsabila Nabilah Rifdah; Agus Sulistyono (Pharmacognosy Journal, 2023)
Background: Antihypertensive agents used during pregnancy may vary from institution to institution and depend on resource availability. Objective: This study aimed to determine the profile of antihypertensive drugs used in pregnancy in low-middle-income countries and the impact of these drugs on maternal and fetal outcomes. Materials and Methods: This is a retrospective study on hypertensive pregnant patients admitted to the emergency unit at a tertiary referral Hospital in Indonesia. The type of hypertension during pregnancy, antihypertensive drugs, side effect, and maternal and perinatal outcomes was extracted from medical hospital records. Results: A total of 762 hypertensive pregnant women were recruited; 61 were diagnosed with preeclampsia, 491 were preeclampsia with severe features and 174 were chronic hypertension superimposed preeclampsia, and 81.54% of them received antihypertension therapy. The most commonly prescribed antihypertensive drugs were combination therapy of nifedipine and methyldopa (96.7%), followed by monotherapy of methyldopa (2.3%), nicardipine (1.1%), and nifedipine (0.2%). Most of the patients successfully attained a Systolic blood pressure<160mmHg. Tachycardia was reported in 47 (7.9%) pregnant women who received antihypertensive medication; none reported hypotension and arrhythmia. Conclusion: Nifedipine and methyldopa are choices for an antihypertensive agent in limited resources, either monotherapy or combined. Nifedipine's immediate release can be used with a low risk of hypotension.
Keywords: Antihypertensive agent; Chronic hypertension; Hypertension during pregnancy; Preeclampsia; Side effect.

Anti-inflammatory Potential of Glycoside Flavonoids from Pterocarpus erinaceus Poir. (Fabaceae) Leaves - Noufou Ouédraogo; W. Leila Marie Esther Belem-Kabré; A.M. Emmanuel Thiombiano; Tata Kadiatou Traoré; Lazare Belemnaba; Moussa Ouédraogo; Innocent Pierre Guissou (Pharmacognosy Journal, 2023)
Background: Chronic diseases have become one of the leading causes of death worldwide in recent years. Despite tremendous advances in the treatments of diseases, several concerns remain. Those with inflammatory components are alternatively treated or completed in Burkina Faso with medicinal plants. The present study was designed to identify and evaluate the anti-inflammatory potential of glycoside flavonoids from Pterocarpus Erinaceus leaves. Methods: The ﬂavonoids contained in Pterocarpus Erinaceus aerial parts were extracted, identified, and characterized. Sequential soxhlet extraction was subjected to preliminary phytochemical screening, and characterization of isolated ﬂavonoid was done by U.V., I.R., 1H & 13C N.M.R. and MS. Subsequently, isolates were tested in vitro for their antiinflammatory potential as well as their antioxidant capacities. Results: Five compounds were determined as corresponding to 3’,4’,5,7-tetrahydroxy flavone (luteolin); quercetin-3-O- sophoroside; quercetin- 3-0-β-glucose (isoquercitrin); Kaempferol-3-O-sophoroside and 3,3’,4’,5,7-pentahydroxyflavone-3- rhamnoglucoside (rutin). Concentrations of quercetin-3-O- sophoroside and quercetin-3-0-β-glucose that inhibit 50% of pro-inflammatory enzymes’ activities were expressed in μg/mL. They were respectively 18.07 ± 0.78 and 32.27 ± 2.02 for xanthine oxidase, 1.12 ± 0.018 and 11.53 ± 0.52 for lipoxygenase, 26.91 ± 0.34 and 19.54 ± 0.25 for acetylcholinesterase. Prevention of the degradation of deoxyribose test gave respectively 19.32 ± 1.08 μg/mL and 26.21 ± 2.25 μg/mL for quercetin-3-O- sophoroside and quercetin-3-O- β-glucose while the anti-DPPH free radical potential was 3.41 ± 0.82 and 2.90 ± 0.18 μg/ mL. Conclusion: These results may justify the traditional uses of P. erinaceus in treating diseases with an inflammatory component.
Keywords: Antioxidant; Compounds; Enzymes; Medicinal plant.

Anti-Neoplastic and Cytotoxicity Potency Measuring of Five Medicago sativa L. (Alfalfa) Leaf Extracts Towards Melanoma (UACC62), Breast (MCF7), Prostate (PC3), and Colon (HCT116) Cancer Cells - Makhele Thapelo Simon; Makhoahle Pakiso Moses; Mashele Sitheni Samson (Pharmacognosy Journal, 2023)
The National Cancer Registry of the National Department of Health of South Africa confirms that in females, the highest incidence of cancers diagnosed in the year 2019 was breast (10 172 cases), cervical (6 945 cases) and colorectal (1 952 cases) cancers, while in males the most diagnosed cancers were prostate, lung, and colorectal neoplasms. The risk factors that promote cancer development, and metastasis or spreading includes excessive alcohol and narcotic usage, carcinogenic infections (oncoviruses) and genetical predispositions. The global incidence of cancer is much likely to increase because of the increased exposure to risk factors (processed fast foods, industrial toxins in air and water) that promote oxidative stress, low grade chronic inflammation, diabetes mellitus, hyperglycemia, and insulin resistance. Because the ancient healer or doctor relied on plants and other natural resources for therapeutic compounds, we wanted to measure the anti-cancer actions induced by our Alfac-facah leaf extracts towards four major cancer cells. The growth inhibitory and cytotoxicity activity that was induced by our extracts was measured using the reliable Sulforhodamine B Assay as per the collaborative research program between the CSIR’s Biosciences Pharmacology Group and the NCI. Our Alfac-facah leaf test material was extracted using five solvents: Ethanol, Methanol, Diethyl-ether, Acetone, and Water. Medicinal Plants persist to play a key role in medicine, whereby they not only help with treating or preventing diseases, but they also contribute to the general wellbeing of the patient. For this reason, natural resources remain a pivotal ingredient of novel drug development compounds
Keywords: AICR (American Institute for Cancer Research); Cancer (malignancy; Carcinoma; CSIR (The Council for Scientific and Industrial Research; ECACC (European Collection of Authenticated Cell Cultures; International Agency for Research on Cancer (IARC); Leukemia; lymphoma); Medicago sativa L. (Alfalfa); NCI (National Cancer Institute; neoplasm; sarcoma; South Africa); Sulforhodamine B Assay (SRB); THC (delta-9 tetrahydrocannabinol). Rick Simpson oil (RSO).; Tumour; U.S Department of Health and Human Services); UK)

Antioxidant Activities, Total Polyphenol Profile and Anticancer Activity, of Leaf, Bulb and Root Extracts of Tulbaghia violacea from Bloemfontein - Pakiso Moses Makhoahle; Dijeng Euginiah Rampana (Pharmacognosy Journal, 2023)
In this study, the effects of the home remedy herb Tulbaghia violacea on antioxidants, total polyphenol activity, and cancer were investigated. Using methanol/dichloromethane and aqueous solvents, the extracts were produced. The antioxidant activity of the extracts was assessed by the 2,2-diphenyl-1- picrylhydrazyl assay, and their phenol content by the gallic acid method. The extracts were found to be inactive or weak against the HeLa (cervix), human cancer cell lines TK-10 (renal), and PC3 (prostate). It is suggested that these three human cell lines be tested against extracts of water and methanol/ dichloromethane at higher concentrations. The plant's leaf extract would also be the best substance to test against the human cell lines TK-10, PC-3, and HeLa. The IC50 values for two to three cell lines show that T. violacea plant extracts (>100 g/ml) have no effect on cells. T. violacea extract has greater antioxidant activity than the control. A thorough phenolic analysis showed that water leaf extract had the highest quantity of phenolics whereas bulb methanol/dichloromethane extract had the lowest. Both the methanol/dichloromethane and the aqueous extracts have the same characteristics for antioxidant activity. In order to enhance food's nutritional content and quality while also supporting excellent health, it has been found that phenolic compounds alter the color, flavor, and other sensory characteristics of the meal. Additionally, they help plants defend themselves against harm from ROS, molecular damage, microbial invasion, insects, and herbivores.
Keywords: Anticancer activity; Antioxidants; Medicinal plants; Polyphenol; Tulbaghia violaceae

Antioxidant, Total Phenolic, and Total Flavonoid of 70% Ethanol Extract of Avocado Seeds (Persea americana Mill.) - Sri Wahyu Ningsih Munthe; Riskianto Riskianto; Denny Juvi; Jessica Novia (Pharmacognosy Journal, 2023)
Avocado seeds (Persea americana Mill.) are high in phytochemicals and are utilized in herbal medicine. The objective of this study is to analyze the antioxidant activities, total phenolics and flavonoids of the P. americana seed extract. P. americana seed extraction was obtained through maceration and reflux using a 70% ethanol solvent. The results obtained were compared in terms of yield productivity, with yields of 43.07 (%) and 39.58 (%) respectively. Phytochemical compounds extracted from P. americana seeds were tested using the phytochemical screening method, the antioxidant activity assay, the total phenolic analysis, and the total flavonoid analysis. The phytochemical screening showed that P. americana seeds contain flavonoids, saponins, phenols, tannins, alkaloids, and quinones. The antioxidant activity of the 70% ethanol extract of P. americana seeds obtained by maceration and reflux method was 77.298 g/mL and 98.626 g/mL, respectively, meanwhile the IC50 values of vitamin C were 12.883 g/mL. The 70% ethanol extract of P. americana seeds obtained by maceration and reflux method had total phenolic content of 276.96 mgGAE/g and 294.96 mgGAE/g, and total flavonoid content of 1.73 mgQE/g and 12.70 mgQE/g respectively. This simply implies that the 70% ethanolic extracts from P. americana seeds obtained through maceration and reflux have strong antioxidant activity.
Keywords: Antioxidants; Avocado seeds; DPPH; Persea americana Mill.; Total flavonoids; Total phenolics.

Anxiolytic-Like Effect of Cymbopogon Citratus (Lemongrass) Essential Oil - Paul Alan Arkin Alvarado-García; Marilú Roxana Soto-Vásquez; Luis Enrique Rosales-Cerquin; Santiago M. Benites; Taniht Lisseth Cubas-Romero; Emilio German Ramírez-Roca (Pharmacognosy Journal, 2023)
Introduction: Essential oils are complex substances that are widely utilized in the practices of aromatherapy. Certain essential oils are recognized for their potential to alleviate anxiety symptoms. This research was conducted to evaluate the effects of Cymbopogon citratus essential oil on anxiety. Methods: The chemical composition of the extracted essential oil was analyzed using Gas chromatography with flame-ionization detection (GC-FID) and Gas chromatography-mass spectrometry (GC–MS). In addition, an experimental study with measures at pretest-posttest was conducted, where 128 participants were divided into two groups, a waiting-list (WL) control group, and an experimental group (EG)treated with aromatherapy based on Cymbopogon citratus essential oil. The anxiety index was evaluated by Zung Self- Rating Anxiety Scale (SAS). Results: The chemical analysis identified geranial (52,1%) and neral (35,2%) as the principal constituents. Anxiety levels decreased in the experimental group during the posttest phase (p<0.05). Besides a large effect size was found for anxiety (d = 0.962; Δ= 0.988) with 1-β=0.999 a percentage of change of -10.99%. Conclusion: Cymbopogon citratus essential oil inhalation showed to be effective in decreasing scores anxiety. Therefore, it has the potential to serve as a supplementary treatment for anxiety.
Keywords: Anxiety; Cymbopogon citratus.; Essential oil

The Aqueous Leaf Extract of Moringa Oleifera had Immunomodulatory Effects on Sheep that had Been Infected by Fasciola Gigantica, Clostridium Novyi Naturally and Impact to Fasciola gigantica Non-Embryonated - Maslichah Mafruchati; Wan Irjani Wan Ismail (Pharmacognosy Journal, 2023)
M., which received a (C), was the study's main objective. The whole thing was eaten. a couple of quotes from (C. dispersed in a liquid. has antibacterial properties (E. elegans). It's necessary to remove the body. C. Eczema on the face is comparable to this. Sheep and novyi both had organic infections. novyi. Following different combinations of treatments, a heavy dose of treatment, and a light dose, fasciolitis manifested in all three groups of infected sheep. The treatment groups received an oral 150 mg/kg aqueous M. oleifera leaf extract every 48 hours for 21 days. Body mass, fecal egg counts, blood anti-Fasciola IgG levels, cytokines (IL-2, IL-17, and IL-10), and C. the elegans' bacterial inhabitants jokingly strike up a conversation with everyone there. Sheep with light and heavy infections gained more weight after receiving M. oleifera treatment and had lower fecal egg counts than control groups; sheep with light infection had a 100% reduction in egg count. The serum concentrations of IL-2, IL-17, and IgG all significantly decreased after M. oleifera treatment. There were significantly higher levels of IL-10 in sheep with both mild and severe infections. C's feces contained noticeably fewer bacteria after taking moringa extract. The two novyi groups had varying degrees of infection severity. Fabricio (F. (as well as OleiferaMdot). C. noteworthy and novyi. M. oleifera aqueous extracts showed a concentration-dependent ovicidal effect on F. gigantica non-embryonated.
Keywords: Anthelmintic; Antimicrobial; Cytokines; Moringa oleifera; Public health.

Assessment of Serum Irisin and Vaspin in Women with Polycystic Ovary Syndrome in Mosul City - Saba Khair Alddin Ibrahim (Pharmacognosy Journal, 2023)
Patients with “polycystic ovary syndrome” (PCOS) may have changes in adipokine levels due to obesity rather than PCOS per se. This should be taken into account as there is a correlation between adipokine levels and BMI. The objective of the subsequent investigation was to measure serum levels of irisin and vaspin in women with PCOS. In the following study, overall, 140 sample size was selected for the evaluation. These 140 were divided into two groups in which 70 women were on the one group which was the intervention group, and 70 women were in control group. The control group of the study were the women who reportedly had regular and normal menstrual cycle. The results indicated high values of vaspin and irisin. The results of this study and previously published literature suggest that both adipocytokines are associated with high blood glucose levels and higher BMI, and finally, both values remain higher in women with PCOS. Vaspin and irisin could be considered as a biomarker for prognosis and therapy followup in patients with PCOS.
Keywords: FSH; Insulin; Irisin; LH; PCOS.; Testosterone; Vaspin

Biological Properties of Polyherbal Formulations: A Review of their Antimicrobial, Anti-Inflammatory, Antioxidant, and Toxicological Activities - Elizabeth Bosede Aladejana (Pharmacognosy Journal, 2023)
The use of traditional and folklore systems of medicine has been an important part of healthcare worldwide, and polyherbal formulations, which are combinations of different herbs, are gaining recognition for their medicinal and therapeutic potential. These formulations can be optimized to have stronger therapeutic effects with lower toxicity. The aim of this study was to review research on the antimicrobial, antiinflammatory, antioxidant and toxicological properties of polyherbal formulations around the world, with a view to promoting their use and identifying areas where further research is needed. The author analyzed 99 articles published between 2010 and 2022, using scientific search engines such as Elsevier, BioMed Central, ScienceDirect, PubMed, and Scopus, to assess the use of polyherbal formulations in different countries. This article enlists some commercial and non-commercial polyherbal formulations all around the world with India leading in the number of publications on polyherbal formulations, followed by Nigeria, and Pakistan. The various pharmacological studies conducted have shown that polyherbal medicines possess antimicrobial, anti-inflammatory, antioxidant and toxicological properties, hence, validating their traditional use. However, further clinical work is required to fully understand the therapeutic potential of polyherbal formulations. The growing interest in the therapeutic use of non-toxic conventional medicinal plants as low-cost alternatives for disease prevention and treatment has made natural products valuable tools for creating new lead compounds and scaffolds. Plants will continue to play a pivotal role in the discovery of drugs for human diseases, and the development of potent cures derived from plants would represent significant progress in the treatment of diseases.
Keywords: Anti-inflammatory; Antimicrobial; Antioxidant; Polyherbal medicines; Toxicological properties.

Briefly Describe the Traditional Preparation Method of Mercury in Mongolian Medicine - Qimuge; Terbish L; Bold Sharavyn (Pharmacognosy Journal, 2023)
Objective: This study aims to review the traditional preparation methods and clinical applications of mercury in Mongolian medicine through a literature review. Methods: Ancient and modern literature on the preparation of mercury were reviewed, and the recorded preparation methods in the literature were summarized and organized. The clinical applications were also briefly described. Results: The traditional preparation methods of mercury in Mongolian medicine can be divided into two parts: preparation before processing and specific processing methods. The preparation before processing includes steps such as removing impurities and reducing the weight of mercury. The "Four Parts Ganlu" emphasizes the toxicity of mercury due to its rust (impurities) and weight. Specific processing methods include hot processing, cold processing, slow processing, and quick processing. According to the clinical application records in the literature, hot processing is widely used and still in use today. As a commonly used mineral medicine in Mongolian medicine, mercury is found in various compound formulations recorded in the literature, with a focus on diseases such as "bile", lymphatic, and brain diseases. The proportion of mercury used varies significantly. For example, the preparation of mercury in the Mongen Wusu-18 pill has a higher content and is widely used with significant effects. In addition, mercury is also used in formulations such as Qishiwu Pearl Pill, Ershiwu Songshi Pill, and Ershiwu Coral Pill. Conclusion: The review and study of the traditional preparation methods of mercury in Mongolian medicine can contribute to the standardization and normalization of processing techniques and provide reference materials for the safe application and formulation of mercury processing products.
Keywords: Literature research.; Mercury; Traditional processing methods

Cadre Interventions on Health Outcomes of Diabetes Mellitus Patients: Systematic Review of Randomized Control Trial - Tini; Darmawansyah; Ridwan Amiruddin; Masni; Anwar Mallongi (Pharmacognosy Journal, 2023)
Background: Diabetes mellitus is a disease that can cause complications in various organs of the body. There needs to be a strategic effort to improve the health outcomes of diabetes mellitus patients. Research studies show that cadres are able to become liaisons between patients and health care providers and make a positive contribution to the surrounding community. This systematic study aimed to review studies on cadre interventions on health outcomes of diabetes mellitus patients. Methods: The literature search was carried out on the online databases of PubMed, Proquest, and Google Scholar journals published in the range of 2015 – 2021. The keywords used included community health worker, intervention, health outcomes, and diabetes mellitus. Meanwhile, the literature criteria used included journal articles, peer-reviewed, written in English, and research studies with the Randomized Control Trial (RCT) design. Result: Based on the results of a review of 15 selected articles, it was found that the overall interventions carried out were providing education, counseling, and group classes. Other activities include home visits, goal setting and action plans, diabetes mellitus diaries, and follow-up phone calls. There were interventions carried out by cadres, in partnership with health care providers and academic partners, involving peers of diabetes mellitus patients and culturally relevant community resources. Conclusion: The results of the review showed that almost all articles found changes in metabolic control, behavior, and psychosocial of diabetes mellitus patients as a result of the interventions. Therefore, there needs to conduct subsequent studies on the effect of cadre interventions on the prevention of disease complications both macrovascular and microvascular.
Keywords: Cadres; Diabetes mellitus; Health outcomes.; Intervention; Roles

Cardioprotective Effects of Thymoquinone on Myocardial Fibrosis - Saugi Abduh; Purwanto Bambang; Dirgahayu Paramasari; Soetrisno (Pharmacognosy Journal, 2023)
Introduction: Thymoquinone (TQ) is one of the active ingredients in herbal plants such as Nigella sativa which has antioxidant and anti-inflammatory properties thus may inhibits cardiac fibrosis formation. This study aims to determine the effectiveness of Thymoquinone as a cardioprotective agent in suppressing the extent of fibrosis in Wistar rats induced with lipopolysaccharide (LPS). Methods: This post-test only control study used 30 Wistar rats which were divided into 5 groups: saline, LPS-induced cardiac fibrosis, LPS-induced cardiac fibrosis treated with TQ 10 mg/mL, LPS-induced cardiac fibrosis treated with TQ 20 mg/mL, and LPS-induced cardiac fibrosis treated with TQ 40 mg/mL. Serum IL-6, GSH, and cTnT levels were measured using ELISA, and Mason's trichrome staining was used to assess myocardial fibrosis. Results: The LPS10+TQ20 and LPS10+TQ40 groups exhibited significantly lower levels of IL-6 compared to the LPS10+TQ10 group (p < 0.05). GSH levels did not show a significant decrease in the TQ groups across different doses (p=0.771). The TQ-treated group demonstrated lower cTnT levels compared to the LPS-only group (p<0.05). Thymoquinone treatment resulted in reduced fibrosis area compared to the LPS10 group (p<0.05). Conclusions: TQ has a promising cardioprotective effect on the formation of cardiac fibrosis in Wistar rats induced with LPS.
Keywords: Cardiac Fibrosis; Lipopolysaccharide; Nigella sativa; Oxidative stress.

Characterization, Preclinical Efficacy and Toxicity Evaluations of Flavonoids Glycosides based Standardized Fenugreek Seed Extract (FEFLG) - Prasad A. Thakurdesai; Pallavi O. Deshpande; Mukul P. Pore (Pharmacognosy Journal, 2023)
Introduction: Fenugreek seeds, a natural food chain raw material, is known to have many flavonoid glycosides. Objective: Characterization, preclinical efficacy, and safety evaluation of flavonoid glycosidebased standardized fenugreek seed extract (FEFLG). Methods: FEFLG was characterized for a group of flavonoid glycoside marker compounds by HPLC. The CD38+ enzyme inhibition efficacy was assessed in vitro. In addition, acute oral toxicity (AOT) and subchronic, 90-day repeated-dose oral toxicity (in vivo), mutagenicity (AMES test, in vitro) and chromosome aberration test (in vitro) of FEFLG were evaluated. Results: The FEFLG was found to have 49.85% of total flavonoid glycosides content in FEFLG (25.15% of Group 1: vitexin, isovitexin and vitexin 2-o- rhamnoside and 24.70% of Group 2 (vicenin derivatives, schaftoside, iso-schaftoside, orientin and iso-orientin). FEFLG showed CD38+ enzyme inhibition in vitro (IC50= 0.96 μg/ml) equivalent to the positive control, apigenin. FEFLG did not show any toxicity at an acute oral dose of more than 2000 mg/kg (median lethal dose, LD50) with a limit dose of 5000 mg/kg. The 90-day repeated-dose oral administration of FEFLG did not induce significant toxicological changes till the maximum dose of 1000 mg/kg in male and female rats, indicating no observed adverse effect level, NOAEL ≥ 1000 mg/kg. FEFLG did not show mutagenicity (up to a concentration of 5000 μg/plate) or structural chromosomal aberrations (up to 5000 μg /ml). Conclusion: The CD38+ enzyme inhibitor efficacy in vitro, oral safety in vivo and absence of mutagenicity or genotoxicity of FEFLG indicated its potential for anti-aging applications.
Keywords: Acute toxicity; CD38+ enzyme inhibition; Chromosomal aberration.; Fenugreek seeds; Flavonoid glycosides; Mutagenicity; Subchronic Toxicity

Child Survival Strategies on the Growth and Development of Infants Aged 0-6 Months in Makassar City (A Study on Employed and Unemployed Mothers) - Arifin Seweng; Nurbaeti; Ella Andayani; Anwar Mallongi (Pharmacognosy Journal, 2023)
The South Sulawesi Provincial Government, through the health Office, stated that South Sulawesi currently has a fairly high infant mortality rate. From January to September 2018, a total of 870 infants died. According to these data, the highest mortality rate was recorded in Bone District. High maternal and neonatal mortality rates have a major impact on families and communities. Therefore, it is highly expected that women's participation as housewives will directly determine the survival of their infants. This study aims to determine the effect of child survival strategies (CSS) on the growth and development of infants of employed and unemployed mothers aged 0-6 months. It is an observational study with a case-control design. This study involved employed and unemployed mothers who had infants aged 0-6 months. The sample was determined by the simple random sampling method in the case and control groups. The Data was collected through interviews using a questionnaire and then analyzed statistically. This study is expected to provide input for the Indonesian Government (Health Office) as a consideration in formulating policies to reduce infant mortality rates, especially in South Sulawesi. The results indicated a significant correlation between mothers' occupations and their infants' fine motor skill development category (p < 0.05 or = 0.033). The anthropometry of infants of employed and unemployed mothers indicated no significant differences, both in terms of age, body weight, and body length (with p > 0.05). There was a significant correlation between mothers' occupations and their infants' fine motor skill development category. The infants of employed mothers tend to have good fine motor skill development than those of unemployed mothers. The percentage of growth and development of other infants that fall into the good category was found to be higher in employed mothers than in unemployed mothers, although it was not statistically significant. Employed mothers have better CSS than unemployed mothers, although it is not statistically significant.
Keywords: Child Survival Strategies; Growth and Development; Infants

Comics in Health as Alternative Therapy: An Information and Therapeutic Resource for Post-COVID-19 Patients in Ayacucho, Peru - Carlos Rodrigo Infante-Yupanqui; Roberta García-de-la-Cruz; Yanibel Hurtado-Vargas; Rosa María Vega-Guevara; Primy Agripina Alca-Chamba; Rod Rosberg Bendezú-Perez; Pavel Pool Puclla-Pareja (Pharmacognosy Journal, 2023)
The aim of this research was to determine the utility of comics, as an informative and therapeutic resource, in post-COVID-19 patients, in the area of influence of the Camisea project, which is delimited to cities such as Huanta, Tambo, and Cangallo, located in Ayacucho, Peru. The methodology included an observational design, with in and out surveys being conducted during the study. The first survey sought to discover the level of approach of post-COVID-19 patients with the use of comics, while the second examined the effectiveness and usefulness after being exposed to a brochure of four comics (Figure 1) that combined humor and information related to the recovery procedures for COVID-19. The research showed that the usefulness of comics as an informative and therapeutic resource in post-COVID-19 patients in the city of Ayacucho is very significant. This was confirmed by 96% of those surveyed, since they pointed out that the comics contributed to virus infection recovery and prevention.

Community Compliance Regarding No-Smoking Area Policy: Belief Control Analysis and Tobacco Use Habits in Society the Bugis Tribe (Pare-Pare City & Sidrap Regency) and the Makassar Tribe (Gowa-Takalar Regency) in South Sulawesi - Achmad R. Muttaqien Al-Madin; H. Amran Razak; Darmawansyah; Suriah; HM Alimin Maidin; Pupin Astuti; Mutia Nur Rahmah; Nur Indah Sari (Pharmacognosy Journal, 2023)
Introduction: Smoking among the people of Bugis-Makassar has become an inseparable part of a smoker and has become a habit. Community compliance with the No-Smoking Area (KTR) policy is still a problem in various regions, this is because the habits and control of people's beliefs are not in line with implementation of KTR implementation. Methods: This study used an explanatory research design, namely to examine the correlation between control beliefs and the habit of using Bugis cigarettes on compliance with the policy of implementing a smoking-free area which is part of tobacco control. The research was conducted in four districts in South Sulawesi, namely Takalar, Gowa, Pare-pare, and Sidenreng Rappang (Sidrap) Regencies. The sample used was 400 respondents using the non-probability sampling method, namely the Accidental Sampling technique. Results: This study shows that control beliefs and respondent compliance obtained a p-value of 0.717 > 0.05 which means there is no significant correlation between control beliefs of tobacco use and community compliance with KTR policies. The results of the correlation test of smoking habits on public compliance with KTR policies obtained a p-value of 0.015 <0.05 which means there is a significant correlation between tobacco use habits and public compliance with KTR policies. The results of the study also show that the control variable beliefs and habits have a p-value of 0.001 <0.05 which means that there is a correlation between control beliefs and smoking habits. Conclusion: Policy makers should focus more on the implementation of KTR policies in all public places and law enforcement related to KTR policies in various contexts, including social and cultural approaches. KTR got easier with time.
Keywords: Believe control; Community compliance; Non-smoking area; Smoking cessation; Tobacco use.

Community Empowerment with Independent Larva Monitor in Reducing the Dengue Hemorrhagic Fever Incidence, in Sidrap Regency - A. Arsunan Arsin; Ridwan Amiruddin; Dian Saputra Marzuki; Rezki Elisafitri; Basir; Anwar Mallongi; Aisyah; Ahmad Yani (Pharmacognosy Journal, 2023)
Objective: This study aims to determine the effectiveness of developing a community empowerment model through independent larva monitor with an overlap in controlling dengue disease incidence in Sidrap Regency. Methods: This study used a mix-method conducted in Tanete Village. The number of samples for quantitative data was 70 respondents, and the qualitative data was 12 informants. Data collection was carried out through Focus Group Discussions, observations, and interviews. The data were analyzed using the McNemar test. Results: The results showed that the behavior of the people affected by dengue fever is people who do not maintain the cleanliness of their environment, litter, and people's habits do not routinely drain water tanks, and drinking places for livestock become mosquito shelters. The results of dengue counseling showed a significant difference between people's behavior related to dengue fever disease before and after counseling. Most people stated that explanations related to how to make and the process of making ovitrap were easy to do. They were interested in making their own because they used materials that were easy to find at home. In addition, the community has been obedient in implementing independent romantic programs. The results of overlap observations show that simple overlap can trap mosquitoes. Conclusion: The development of empowerment models through independent larva monitor can be used to prevent and control dengue disease.
Keywords: Community Empowerment; Dengue fever; Independent Larva Monitor; Ovitrap

Comparative and Evaluative Study On Total Phenolic Content and Antioxidant Potential Within Hydromythanol Extracts and Their Fractions from All Parts of Drimia numidica (JORD. & FOURR.) J.C. MANNING & GOLDBLATT of Northeastern Algeria - Fares Khenniche; Abderachid Slimani; Chawki Bensouici; Ibtissem Magboune; Manel Srief; Hamed Hakkom (Pharmacognosy Journal, 2023)
Introduction: Drimia numidica is a polyphenol-rich species that has antioxidant capacity, which makes it very important. Nevertheless, D. numidica has not been studied in depth. Moreover, its capsules have not been studied so far. This detailed study compared the values of total phenolic content and antioxidant activity of all D. numidica parts (scapes, flowers, bulbs, capsules, leaves, roots), from Edough Peninsula, Annaba, Algeria, using hydromethanol extracts and their fractions (cyclohexane, chloroform, ethyl acetate, and n-butanol). Methods: The total phenolic content was determined by Folin Ciocalteo assay, and the antioxidant activity by the following methods: DPPH, Reducing Powder, Phenanthroline, Silver Nanoparticles, CUPRAC, and ABTS. Statistical analysis was done using the ANOVA test and a correlation test (between antioxidant activity and total phenolic content). Results: The Ethyl acetate fraction was found the sample with the highest phenolic content. The same was true for the antioxidant activity in all tests except for the silver nanoparticles test in which cyclohexane extracts scored the best. Considering the methods used in general, there is a correlation between phenolic content and the highest antioxidant activity, but there are some methods that have not recorded any correlation. Conclusion: Choosing the appropriate method or extract/fraction type is extremely important. In addition, extracts of D. numidica parts could be an important natural alternative to antioxidants industrial.
Keywords: antioxidant activity; Drimia numidica; Hydromythanol extracts and their fractions.; total phenolic content

Comparative Evaluation of Microhardness and Color Change of Root dentin using Punica granatum (pomegranate extract), Sodium hypochlorite, Chlorhexidine and Normal saline as an Endodontic irrigant – An in vitro study - Ankita singh; Sunil Tejaswi; Kenganora Mruthunjaya; Suneeth Shetty; Ambikathanaya U.K; Sachin B. Manglekar (Pharmacognosy Journal, 2023)
Aim: To evaluate the microhardness and change in color of root dentin using Punica granatum, sodium hypochlorite, chlorhexidine and normal saline as an endodontic irrigant and to implement their use in field of dentistry. Materials and Methods: 60 single rooted anterior teeth extracted due to periodontal and orthodontic reasons were collected. All the samples were decoronated to achieve constant length of 14mm. Patency of root canal was established using 10k file and working length was determined 1mm short of the file length. Each canal was prepared till F3 protaper. After preparation the samples were randomly divided into 4 groups: Group 1: Saline (Control); Group 2: 5% Sodium Hypochlorite; Group 3: 2% Chlorhexidine; Group 4: 5% Punica granatum (pomegranate peel extract). After grouping the samples were longitudinally sectioned. One half of the tooth sample was used for microhardness testing using Vickers microhardness indentor and the other half of sample was used for color change evaluation using spectrophotometer after irrigation with the test solutions on day 1 and day 7th post irrigation. Results: The mean microhardness in all four groups decreased comparatively at post as compared to pre and the decrease was evident highest in sodium hypochlorite followed by chlorhexidine, pomegranate and saline the least. In regard to color change sodium hypochlorite, chlorhexidine and pomegranate all showed visible color changes after 7 day of irrigation with pomegranate showing the highest color change. Higher lightness was shown in Group 2 i.e., sodium hypochlorite whereas redness was seen more in Group 4 i.e., pomegranate and yellowish tint was more visible in Group 3. Conclusion: 5% Punica granatum has showed negligible effect on microhardness of dentin but showed maximum color change when compared to chlorhexidine, sodium hypochlorite and saline.
Keywords: Chlorhexidine.; Color change; Endodontic irrigant; Herbal; Microhardness; Punica granatum (pomegranate peel extract); UVVIS spectrophotometer

A Comparative Study of CFA and MIA Induction Models in Rat Knee Arthritis - Rena Normasari; Bambang Purwanto; Damayanti Tinduh (Pharmacognosy Journal, 2023)
This study presents another comparative review of knee joint arthritis induction in rats using two distinct methods: Complete Freund's Adjuvant (CFA) and monosodium iodoacetate (MIA). Different variables are assessed, including bodyweight changes, knee bend scores, and knee diameter measurements, as well as the quantification of interleukin-1β (IL-1β) and C-telopeptide of type II collagen (CTX-II) levels. CFA or MIA induction was used on rats, and 14 days were observed. Our data show that the impact of arthritis induction varies significantly across the two models. Both the CFA and MIA groups showed different changes in terms of bodyweight changes, knee bend scores, and knee diameter variations. Furthermore, the levels of IL-1β and CTX-II, both known indicators of inflammation and cartilage degeneration, were measured. Notably, IL-1β levels in the CFA group were considerably higher than in the MIA-induced rats, although CTX-II concentrations showed a contrary pattern. These findings highlight the need to carefully consider the induction approach when performing arthritis investigations in rats since the model used has a major impact on the reported physiological alterations. This study's comparative analysis provides useful information for researchers looking to use rat knee joint arthritis models, laying the groundwork for a better-informed selection of the best induction strategy depending on desired outcome metrics.
Keywords: CFA; CTX-II; IL-1β; MIA; Osteoarthritis

Comparative Study of Polyherbal Formulation for Antiarthritic Activity Having Cockle Shell, Egg Shell, Ginger and Balloon Vein in Gel Form and Oil Form: A Novel Preparation for Anti-Oxidant Activity - S. Nithya; S. Dhanalakshmi; S. Anand Babu; S. Nirmala; D. Bharathi; L. Karpagavalli (Pharmacognosy Journal, 2023)
Inflammatory and chronic disease of the joints and tissues surrounding them, rheumatoid arthritis is known as the most common form of arthritis. Traditional medicines plays major role because of more advantageous like lesser side effects, naturally available and cost effective. A formulation for anti arthritis activity was developed, isolated, and evaluated in this study. Based on the extensive review of the literature, we have formulated three formulation like gel, polyherbal oil formulation with extract of herbs and polyherbal formulation with powders of herbs. We have selected, traditional herbs (Cockle shell, Egg shell, Ginger and Ballon Vein) based on the literature and does a comparative study between gel and the oil formulation to check which has better anti arthritis activity. The selected herbs for formulation of gel are cockle shell and egg shell which has rich calcium content and for oil formulation herbs like ballon vein and ginger were chosen.The chemical constituent present in herbs plays a major role in curing rheumatoid arthritis.Then finally we have done a evaluation like ph measurement, spreadability, specific gravity, antioxidant study etc., between the comparison of DPPH assay of the formulation, clearly reported that the efficacy in the medicated oil in the extract and well in the macerated oil showed significant antioxidant activity when compared to the gel.
Keywords: Herb formulation; In vitro studies – DPPH.; RA

Comparison of Antibacterial and Antioxidant Activities of Ethanolic Extracts of Four Plant Species Selected from South of Saudi Arabia - Ali Abdallah Alqudah; Bilal Al Hawamdeh; Dahfer Ali; Ibrahim Alfarrayeh; Bilal Algataitat; Omar Khaled Al-Mobideen; Mohammad Alhawatema (Pharmacognosy Journal, 2023)
One of the most ancient human medical techniques is the use of plants to treat, prevent, and cure diseases. These plants can manufacture a wide variety of natural chemicals. The present study aimed to evaluate the antibacterial activity and antioxidant capacity of ethanolic extract of four plant species (Zizyphus lotus, Lavandula dentata, Ruta graveolens, and Dodonaea viscosa). Using disc diffusion and serial dilution procedures, the antibacterial abilities of these EtPEs were evaluated. The antioxidant properties were evaluated by the FRAP method and the Folin-Ciocalteu technique was used to measure the total phenolic content. Different plant extracts showed different inhibitory effects on the tested bacteria in a dose-dependent manner. Among the tested plant extracts, D. viscose exhibited the highest antibacterial activity against P. vulgaris and S. aureus, with a minimum inhibitory concentration (MIC) value of 0.5 mg/ml. On the other hand, R. graveolens displayed the highest quantity of phenolic compounds and demonstrated the highest antioxidant activity. Notably, there was a positive correlation observed between the antioxidant activity of the plant extracts and their total phenolic content. In conclusion, the findings of this study suggest that the tested plant extracts hold potential as promising sources of natural antibacterial and antioxidant agents.
Keywords: Antibacterial; Antioxidants; Extraction; Medicinal Plants.; Total Phenols

Comparison of Hb Levels Given Iron Supplements with and Without Vitamin B12 to Anemia Female Industrial Workers in Surakarta Residency Area - Niken Pratiwi; Rutmauli Hutagaol; Marwansyah; Muhammad Pahruddin; Erna Fauziah; Parellangi; Anwar Mallongi; Sukri Palutturi (Pharmacognosy Journal, 2023)
Objective: The research objective was to analyze the comparison of Hb levels given iron supplements with and without vitamin B12 in female industrial workers who experience anemia. Method: The study used an experiment with a pretest-posttest control group design. The research respondents were female industrial workers who experienced anemia in the Surakarta Residency Area. The sampling method was carried out by purposive sampling. The number of samples was 33 respondents divided into a treatment group of 17 respondents and a treatment group of 16 respondents. Results: There was no significant difference in hemoglobin levels in the control group and the treatment group as indicated by a p>0.05. The results of the paired t-test statistic showed that there was a significant change in hemoglobin levels in the treatment group (0,96 ± 0,40) p 0,000 (p<0,05). Conclusion: Giving iron supplementation to anemic women workers can increase hemoglobin levels. Handling of anemia in female workers can be done by making iron supplementation programs and policies in all companies that employ female workers.
Keywords: Anemia; Iron Deficiency; Iron Supplements; Vitamin B12; Women Industrial Workers

Comparison of the Anti-Inflammatory Activity of Flavonoid Bioactive Compounds Acetone Fraction and Steroid Fraction Ethyl Acetate Sungkai Leaves In Vivo and In Silico Studies - Madyawati Latief; Rahmani; Arliangga Fahrezi; Sutrisno; Indra Lasmana Tarigan (Pharmacognosy Journal, 2023)
One of the medicinal plants that is widely used by traditional communities is sungkai (Peronema canescens Jack), which is known to contain secondary metabolite compounds of flavonoids, saponins, tannins, steroids, terpenoids, alkaloids, and phenols. This research aims to isolate secondary metabolite compounds from acetone and ethyl acetate fractions, which act as anti-inflammatory agents in vivo and in silico. Acetone and ethyl acetate fractions were isolated and characterized using UV-Vis and FT-IR. The In Vivo test was carried out on Mus musculus, while the In Silico test was carried out using the IL-10 and IFN-γ receptors, which play a role in inflammation and immunity. Our research results showed that the ethanolic (F1) and n-hexane (F2) fractions of P. canescens extract had good anti-inflammatory activity with percent inhibition values of 58.12% and 56.59%, respectively. The characterization results showed that isolate F1 was a flavonoid group, Naringenin compound, while isolate F2 was a steroid group, β-Sitosterol compound. Moreover, from the scoring docking results, β-Sitesterol has tremendous potential as an anti-inflammatory than the compound naringenin.
Keywords: Anti-inflamatory; β-Sitesterol; Naringenin; P. canescens Jack

Complete Blood Profile after administration of Hydrocotyle sibthorpioides Lam. extract in capsule form - Afriwardi; Adrul Fauzan; Salman Umar; Yufri Aldi (Pharmacognosy Journal, 2023)
Pegagan embun (Hydrocotyle sibthorpioides Lam.) has been studied as an immunostimulant, increasing macrophage cell activity and phagocytosis capacity. Based on that circumstance, the study aims to prove the immunostimulating effect by measuring the activity of Hydrocotyle sibthorpioides Lam. extract in the capsule for the complete blood profile of healthy volunteers. The number of volunteers used was twenty people, and the volunteers were divided into two groups. The first group was given a placebo capsule without Hydrocotyle sibthorpioides Lam. extract. The second group was given a capsule with Hydrocotyle sibthorpioides Lam. extract with a dose of 67 mg, which was taken once a day for three days. Blood sampling was obtained before and after taking the capsule preparation. Observation of the complete blood profile was conducted by investigating changes in blood parameters such as haemoglobin levels, number of erythrocytes, number of leukocytes, hematocrit values, number of thrombocytes and the percentage of leukocyte types (banded neutrophils, segmented neutrophils, eosinophils, basophils, monocytes, and lymphocytes). The second group given Hydrocotyle sibthorpioides Lam. extract showed a significant effect on the increase in haemoglobin levels, number of thrombocytes and hematocrit values (p<0.05). The percentage of leukocyte type values showed that lymphocytes increased significantly (p<0.05). In contrast, the segmented neutrophil increased but did not show a significant difference with the percentage of banded neutrophils, eosinophils, basophils and monocytes (p>0.05). There was a nonsignificant result in all parameters for the first group, which was administrated with a placebo capsule without Hydrocotyle sibthorpioides Lam. extract.
Keywords: Banded neutrophil; Basophils; Eosinophils; erythrocytes; Haematology; Haemoglobin; Hematocrit; Hydrocotile sibthorpioides Lam.; Immunostimulants; Leukocytes; Lymphocyte; Monocytes; Segmented neutrophil; Thrombocytes.

Computational and Pharmacokinetic Investigation of Some Heterocyclic Amide Derivatives as Cyclooxygenase Inhibitors: An In-Silico Approach - Emranul Kabir; M. R. O. Khan Noyon; Monir Uzzaman (Pharmacognosy Journal, 2023)
The two most significant as well as historically important non-steroidal and anti-inflammatory medications (NSAIDs), aspirin and ibuprofen, are frequently used to treat fever, pain, and inflammation. By blocking the activity of cyclooxygenase (COX), it can prevent the production of prostaglandin. In an effort to examine the physiochemical and biological properties of some heterocyclic amide derivatives and quantum mechanical computations have been used to analyze the compounds. To clarify the thermochemical, molecular orbital, and equilibrium geometrical features in the gas phase, density functional theory (DFT) with the B3LYP/6- 31G basis set has been used. Binding affinities and modes of heterocyclic amide analogs have been investigated on human cyclooxygenase (COX-1 and COX-2) proteins (6Y3C and 5F19) using molecular docking as well as nonbonding interactions. Results from geometry and thermochemical analysis support the chemical sustainability of all the structures. Most of the compounds exhibited a significant affinity for binding to the receptor protein (5F19) than the standard drugs aspirin and ibuprofen. The improved pharmacokinetic features of certain derivatives with reduced acute oral toxicity were revealed by ADMET prediction. Overall, four heterocyclic amide analogs 3-6 were found to be more efficient in inhibiting COX- 2 (5F19) than COX-1 (6Y3C), suggesting that they may be useful as COX-2-related inflammation drug candidates.
Keywords: ADMET.; Cyclooxygenase (COX); Heterocyclic amide derivatives; Molecular docking

Computational Evaluation of the Potential of Salicylate Compound from Syzygium aromaticum on Carbonic Anhydrase I as a Gastric Acid Stimulant - Rahadian Zainul; Rismi Verawati; Rauza Sukma Rita; Fadhli Ranuharja; Musa Ghufron; Agariadne Dwinggo Samala; Herland Satriawan; Muhammad Raffi Ghifari; Devi Purnamasari; Riso Sari Mandeli; Amalia Putri Lubis; Viol Dhea Kharisma; et al. (Pharmacognosy Journal, 2023)
This article explores the potential of the salicylate compound (Syzygium Aromaticum) as a stimulant for Carbonic Anhydrase I in gastric acid secretion, using a computational approach. The research methods include molecular modeling with Pymol and Pyrex, determination of compound structure and interactions with Protein Plus, and examination of physicochemical properties using the Lipinski Rule. The results show that the Binding Affinity of salicylate with Carbonic Anhydrase I ranges from -7.3 to -6.5, with RMSD values of 0, 2.102, and 2.212, indicating good modeling quality. The interaction between salicylate and Carbonic Anhydrase I is also supported by the findings from Protein Plus. Furthermore, the salicylate compound complies with the Lipinski Rule, with a molecular weight of 137, 1 hydrogen bond donor, 3 hydrogen bond acceptors, a log P value of 0.34, and a molar reactivity of 34.16. This study highlights the prospect of salicylate as a potential modulator of Carbonic Anhydrase I.
Keywords: Carbonic Anhydrase I; Gastric Acid Stimulant; Molecular docking; Salicylate; Syzygium Aromaticum.

Conformity of Differential White Blood Cell (WBC) Examination Results on the Sysmex DI-60 And Sysmex XN-1000 at Prof. Dr. IGNG Ngoerah General Hospital, Denpasar, Bali - I Dewa Komang Agung Cahyadi; I Nyoman Wande; Ni Nyoman Mahartini (Pharmacognosy Journal, 2023)
Background: Sysmex DI-60 and Sysmex XN-1000 are automatic analyzers that can be used to check WBC differential counts. Ensuring the suitability of the WBC differential count results from the two tools can help in daily practice. Objective: Knowing the suitability of the WBC differential count examination results on the Sysmex DI-60 and Sysmex XN-1000. Method: Observational analytical research with a cross-sectional design. The data analyzed were differential WBC count data from samples of babies who underwent Complete Blood Count examinations on the Sysmex XN-1000 device and IT Ratio on the Sysmex DI-60 device without intervention (pre-classification results) at the Clinical Pathology Laboratory, Prof. Head overMarch 2023 – April 2023. Results: 81 samples were analyzed. The neutrophil value on the Sysmex DI-60 and Sysmex vs 34.15% ± 14.83%, median monocytes 4.00% (0.00-30.00)% vs 13.05% (1.30-34.40) %, median eosinophils 3.50% (0.00-31.00)% vs 3.75% (0.00-29.90)%, and median basophils 0.75% (0.00-16.00)% vs 0.30% (0, 00-2.90)%. The eosinophil variable did not show a significant difference between the results of the Sysmex DI-60 and Sysmex XN-1000 examinations (p=0.081). The results of the correlation analysis showed that there was a significant, strong, positive correlation between Sysmex DI60 and Sysmex. Strong positive significance in the lymphocyte variable (r=0.818). However, no significant correlation basophil variable (r=0.044). Bland-Altman analysis shows that the most appropriate differential count examination is the eosinophil variable. Conclusion: Automatic WBC analyzers apply not only one examination method to detect WBC. The use of the Sysmex DI-60 and Sysmex XN-1000 provides automation for checking the WBC differential count with different working principles. In this study, there was only agreement between the differential count results for the eosinophil variable, so when operating these tools, intervention from a Clinical Pathologist was still required.
Keywords: DI-60; Differential Count; WBC; XN-1000

Correlation Between Reticulocyte Hemoglobin Equivalent (RetHe) With Erythrocyte Indices in Predialytic Chronic Kidney Disease Patients in Prof. Dr. I.G.N.G. Ngoerah General Hospital Denpasar - Christina Noviasiata Djawa; Sianny Herawati; Ni Kadek Mulyantari (Pharmacognosy Journal, 2023)
Background: Early detection and monitoring of anemia are important in CKD patients, which significantly increases the effectiveness of clinical treatment, reduce treatment cost and improve quality of life patients. Differential diagnosis of anemia based on conventional erythrocyte indices is limited, therefore it requires to be combined with other indices to determine iron deficiency. Reticulocyte hemoglobin equivalent (RET-He) is an alternative indicator of iron deficiency that is not affected by inflammation, and has been proposed as an additional marker for iron deficiency screening. Objective: To determine the correlation between RET-He and erythrocyte indices (MCV, MCH, MCHC) in predialytic CKD patients. Method: Cross-sectional retrospective study of predialytic CKD patient data in Prof. Ngoerah General Hospital, that were tested for RET-He and erythrocyte indices in January – March 2022. Data was collected from laboratory information system (LIS) and medical record. RET-He was measured with lightscatter flow cytometry method and erythrocyte indices was calculated. Result: Sixty and three (63) study subjects were consisted of 37 men (58,7%) and 26 women (41,3%), with average of age was 60,68±10,52 years. The mean value of RET-He, MCV, MCH and MCHC was 30,55±2,86 pg, 89,08±7,78 fL, 28,76±2,72 pg, and 30,55±2,86 g/dL. Result of data analysis showed positive correlation between RET-He and MCV (r = 0,659), MCH (r = 0,734), and also between RET-He and MCHC (r = 0,383), with p < 0,05. Conclusion: There was a significant positive correlation between RET-He with erythrocyte indices in predialytic CKD patients.
Keywords: Erythrocyte indices; Predialytic CKD; RET-He

Correlation of Apo E Gene Polymorphism with Recurrent Acute Coronary Syndrome - Muhamad Robiul Fuadi; Jusak Nugraha; I Gde Rurus Suryawan; Hartono Kahar; Aryati; Gwenny Ichsan Prabowo; Budi Utomo; Reny I’tishom (Pharmacognosy Journal, 2023)
Background: Recurrent cardiovascular disease (CVD) incidence puts patients at higher risk for mortality and morbidity. One of the CVD symptoms is acute coronary syndrome (ACS). Many genetic polymorphisms are CVD risk factors. This study’s purpose was to investigate the correlation between recurrent ACS incidence and apolipoprotein E (ApoE) gene polymorphism. Method: Case-control design was used in this study. About 90 patients who visited the cardiology and internal medicine clinics at UNAIR Hospital in Surabaya, Indonesia, served as the study's subjects. There were 30 patients with recurrent ACS, 30 patients with a single ACS, and 30 patients with no history of cardiovascular disease. Afterward, using the polymerase chain reaction-restriction fragment length method, the ApoE gene polymorphism examination was carried out. The Tropical Disease Center UNAIR Laboratory conducted all laboratory testing. Results: In the recurrent ACS group, ApoE polymorphism genotype patterns were 5 subjects for ε2ε2 (16.67%), 23 subjects for ε3ε3 (76.66%), and 2 subjects for ε4ε4 (6.67%). Meanwhile, in the single ACS group, ApoE polymorphism genotype patterns were 6 subjects for ε2ε2 (20%), 22 subjects for ε3ε3 (73.4%), 1 subject for ε4ε4 (3.33%), and 1 subject for ε2ε3 (3.33%). And, in the non- ACS group, ApoE polymorphism genotype patterns were4 subjects for ε2ε2 (13.34%), 25 subjects for ε3ε3 (83.33%), and1 subject for ε4ε4 (3.33%). There was no correlation of ApoE gene polymorphism with recurrent ACS incidence by Chi-square analysis (p > 0.05). Conclusion: ApoE gene polymorphism cannot significantly affect recurrent ACS incidence.
Keywords: Acute Coronary Syndrome; ApoE gene; PCR RFLP; Polymorphism; Public Health

Correlation of Mitotic Index (MI) and Tumor Infiltrating Lymphocytes (TILs) to Chemotherapy Response in Triple Negative Breast Cancer (TNBC) at Haji Adam Malik General Hospital Medan - Kamal Basri Siregar; Barry Winaldy Siregar; Suyatno (Pharmacognosy Journal, 2023)
Background: The response to chemotherapy depends on the proliferation of cancer cells. The higher the proliferation of cancer cells, the better the response. Mitotic Index and Tumor Infiltrating Lymphocytes are markers related to the rate of proliferation and cancer immunity. Objectives: to analyze the correlation of Mitotic Index (MI) and Tumor Infiltrating Lymphocytes (TILs) to chemotherapy response in Triple-negative breast cancer (TNBC). Methods: This study used an observational analytic design with a cross-sectional approach. It was conducted at the Division of Oncology Surgical, Department of Surgery, USU Medical Faculty, H. Adam Malik Hospital, Medan, for six months with a sample of 60 people. Results: On average, most respondents are 47 years old, with the youngest age being 27 and the oldest 73 years old. Most respondents have Grade II, with most in stage IIIB. There was no relationship between Tumor Infiltrating Lymphocytes and Chemotherapy Response (p>0.05), with a Positive Predictive Value (NPP) of 47% and a Negative Predictive Value (NPN) of 50%. Conclusion: There is no relationship between the mitotic index and clinical response to chemotherapy, and there is no relationship between tumor-infiltrating lymphocytes and the response to chemotherapy due to influencing factors such as albumin, T stage, menopausal status, and receptor status.
Keywords: Chemotherapy response; Mitotic index; TNBC; Tumor-infiltrating lymphocytes

Correlation of TB LAM Ag (Tuberculosis Lipoarabinomannan Antigen) Results from Urine with Adenosine Deaminase Levels from Pleural Fluid Patients with Pulmonary TB Accompanied by Pleural Effusion - Sari Prabandari Prasetyaningrum; Arief Bakhtiar; Yessy Puspitasari (Pharmacognosy Journal, 2023)
Background: One of the newest testing methods for active TB uses TB LAM Antigen, in which lipoarabinomannan (LAM) as the examined substance serves as the main component of the cell wall of M. tuberculosis (MTB) and the degradation product of MTB by macrophages in the human body. Patients with active TB with or without HIV infection may experience a decreased antibody response, which causes LAM not to bind to antibodies so that it can pass through the normal glomerular basement membrane and can be detected in the urine. Lateral flow urine lipoarabinomannan assay (LF-LAM) can detect this material with urine samples from active TB patients. Another TB screening method uses the measurement of adenosine deaminase (ADA) activity. ADA has a very important function for the proliferation and differentiation of lymphoid cells, especially T cells, and assists in the maturation of monocytes into macrophages. Objective: This study aims to qualitatively prove the correlation between the pleural fluid ADA levels of pulmonary TB patients with pleural effusion using the LF-LAM test results and examine the potential of TB LAM Ag, which correlates with increased pleural fluid ADA levels, as a diagnostic tool for diagnosing pulmonary TB. Method: It is an observational analytic study with a crosssectional design. Materials for this study included temporary urine from active TB patients at Dr. Soetomo Regional Public Hospital collected in May-August 2022. The descriptive analysis was conducted using SPSS 25.0. The data were tested for normal distribution and with the homogeneity test of Shapiro-Wilk's data. The statistical analysis was performed using the Mann-Whitney test, while the kappa suitability test was carried out to determine the correlation and level of concordance between the ADA cut-off value of pleural fluid and the TB LAM Ag test results. Results: This study involved 50 subjects consisting of 22 (44%) subjects with ADA test results between 40-100 IU/L compared to 28 subjects (56%) with ADA test results below 40 IU/L. There were 14 (28%) subjects with positive TB LAM Ag test results, consisting of 10 (45%) active TB subjects with a high ADA test cut-off of 40-100 IU/L and 4 (14%) active TB subjects with a low ADA test cut-off below 40 IU/L, with a p-value = 0.012. Conclusions: There is a correlation between TB Lipoarabinomannan antigen test results of TB patients with pleural effusion. TB LAM Ag can be detected positive on ADA test results of < 40 IU/L and more positive in active TB patients with high ADA test results of > 40-100 IU/L. Therefore, the TB LAM Ag test is more sensitive than the ADA test. The cut-off value of the ADA test reached 76.4 U/L, and a positive TB LAM was found.
Keywords: Active TB with pleural effusion.; Adenosine deaminase (ADA) test; TB Lipoarabinomannan (LAM) Antigen

Correlation of Tumor Infiltrating Lymphocytes (TILs) to Apoptotic Index (AI) in Breast Cancer - Kamal Basri Siregar; Barry Winaldy Siregar; Suyatno (Pharmacognosy Journal, 2023)
Background: TILs is higher in breast cancer tissue, especially in the stroma compared to normal breast tissue. There is still no research on the relationship between AI and TILS in breast cancer. Yet another study indicating the presence of lymphocytic infiltration was investigated further by detailed analysis of apoptotic epithelial/tumor cells, using the CytoDEATH M30 antibody. The number of apoptosis was significantly higher. Methods: This study used an observational analytic design with a cross-sectional approach to analyze the relationship between Tumor infiltrating lymphocytes (TILs) and the apoptotic index (AI) in breast cancer. This research was conducted at H. Adam Malik General Hospital. The study was conducted using a case series model to see the correlation between Tumor Infiltrating Lymphocytes (TILs) and Apoptotic index (AI) in Breast Cancer. Results: In this study there were 52 patients where the mean age was 54.8 years with a standard deviation of 9.66 years Based on the characteristics of the TNM, it was found that most of the patients came with T3 (24 patients/46.2%), N0 (29 patients/55.8%) and M1 (31 patients/59.6%). Based on the frequency, there were 35 patients with high TILS or 67.3% of the total sample and 44 patients with high AI or 84.6%. In this study, an analysis of the relationship between TILS and AI was carried out where a p value <0.001 was obtained indicating a significance and relationship between TILS and AI. Conclusions: There is a significant relationship between tumor infiltrating lymphocytes (TILs) and the Apoptotic index (AI).
Keywords: Apoptotic Index; Breast cancer; H. Adam Malik General Hospital; Tumor Infiltrating Lymphocytes

Curcumin from Curcuma longa L. as Dual Inhibitors Against Indonesian SARS-CoV-2 Isolates: A Molecular Docking Study - Chairul A. Nidom; Arif N. M. Ansori; Astria N. Nidom; Setyarina Indrasari; Reviany V. Nidom (Pharmacognosy Journal, 2023)
COVID-19 has become a global pandemic since 2020. The search for promising drugs based on the abundant herbal ingredients in Indonesia is one of the breakthroughs. Curcumin is a chemical compound with various potentials such as antioxidant, anti-inflammatory and antiviral. We conducted a molecular docking analysis to determine the potential of curcumin against SARS-CoV-2 non-structural and structural proteins, such as the main protease and spike protein. This study used the compound of curcumin (PubChem CID: 969516) from Curcuma longa L. or turmeric and two Indonesian SARS-CoV-2 isolates that have been deposited in the GISAID database (hCoV-19/Indonesia/JI-PNF-217315/2021 - EPI_ ISL_12777089 or lineage B.1.617.2 and hCoV-19/Indonesia/JI-PNF-211373/2021 - EPI_ISL_6425649 or lineage B.1.470). In addition, we used molnupiravir (PubChem CID: 145996610) as a drug control. We performed molecular docking analysis with PyRx software 0.9.9 (academic license) and visualization of molecular docking results with PyMOL software 2.5.4 (academic license). The results of this study found that curcumin had good potential against main protease and spike protein compared to the drug (control). In summary, we suggested that curcumin is a potential drug candidate against SARS-CoV-2. However, there is a need for future wet laboratory-based pre-clinical research such as in vitro and in vivo.
Keywords: COVID-19; Curcumin; Indonesia; Molecular docking; SARS-CoV-2

Decreased Expression of Endocrine Glands Vascular Endothelial Growth Factor (EG-VEGF) in Rat Endometrial After Stimulation with Recombinants FSH Can be Reduce Implantation Rates - Raden Muharam; Nurhuda Sahar; Kusmardi Kusmardi; Luluk Yunaini; Risqa Novita; Rosalina Thuffi; Conny Riana Tjampakasari; Ponco Birowo; Diyah Kristanty (Pharmacognosy Journal, 2023)
Background: Decreased expression of EG-VEGF in human endometrium after administration of ovarian stimulators has been reported to adversely affect endometrial receptivity and low implantation rates. Objective: To determine the effect of recombinant FSH administration on EG-VEGF protein in the endometrium of rats taken in the secretory phase and how it relates to endometrial receptivity. Design: A total of 36 female wistar rats with normal estrous cycles were randomly assigned to the natural cycle group (NC) and two stimulation groups (SC) which were injected with recombinant FSH at 12.5 IU and 25 IU intraperitoneally. Uterine necropsy and blood collection were performed on day 1, day 2, and day 3 after hCG administration. A total of 3 female rats from each group were mated with male rats (two males and three females in one cage). A successful marriage is indicated by the presence of a vaginal plug the next day. The level of EG-VEGF protein expression was assessed by immunohistochemical technique and steroid hormone levels were measured by the Elisa technique. Results: ANOVA test, that the expression of EG-VEGF in the endometrial glands showed a significant decrease from the normal cycle group to the stimulated cycle group 1 (SC 1) and SC2 (P = 0.00), as well as the expression of EG-VEGF in the endometrial stroma. (P = 000). Steroid hormone levels did not show a significant decrease between the normal cycle group and the stimulated cycle group (P = 0.48 and P = 0.13). Conclusion: Decreased EG-VEGF expression in rat endometrium after administration of recombinant FSH is associated with decreased endometrial receptivity which can reduce pregnancy rates.
Keywords: EG-VEGF; Endometrial receptivity; rFSH; Secretory phase.

Description of Ciplukan Toxicity (Physalis angulata L.) - Ruqiah Ganda Putri Panjaitan; Titin; Yohanes Gatot Sutapa Yuliana (Pharmacognosy Journal, 2023)
Introduction: Ciplukan (Physalis angulata L.) is a plant used by Indonesian people as traditional medicine. Drug sanitization needs to be carried out to guarantee the use and availability of scientifically safe traditional medicines. This study aims to provide information and enrich knowledge about the safety of consuming ciplukan roots and stems. Methods: This study used 8 male mice as test animals, divided randomly into 4 treatment groups, namely mice treated with a dose of 0.56 mg/20 g body weight; mice treated with a dose of 5.6 mg/20 g body weight; mice treated with 56 mg/20 g body weight; and mice treated with 560 mg/20 g body weight. Treatment was given once and then observed for 24 hours to observe the number of deaths of the test animals. Then follow-up observations were carried out in 3 days on individuals who were still alive. Results: Within 24 hours all individuals at the treatment dose of 0.56 mg/20 g body weight survived, whereas all individuals at the treatment dose of 5.6; 56; and 560 mg/20 g body weight died. The observations on individuals treated at a dose of 0.56 mg/20 g body weight showed that the animals were in good condition, with sleeping and eating activities, moving a lot, having clean and nice fur, and not showing toxic symptoms such as disturbances in physical activity, impaired balance, and refusal to eat. Conclusions: The administration of the test extract below is less or equal to 0.56 mg/20 g body weight is relatively safe.
Keywords: Acute toxicity; Ciplukan; Condition; Dosage; Traditional medicine.

Development and Evaluation of Bio fabricated Silver Nanoparticles from Blumea lacera for In-vitro Antibacterial, Antioxidant and Anti-inflammatory Activity - Tarkeshwar Dubey; Kancharla Bhanukiran; Kuna Das; Siva Hemalatha (Pharmacognosy Journal, 2023)
Background: Increasing prevalence of microbial resistance and side effects of currently available drugs compels the researchers to look for alternate therapies and formulations to overcome this problem. Plant based formulations have been proved to be most reliable agents in recent times. Objective: In the current study, bio fabricated herbal silver nanoparticles (HSNPs) were prepared by reducing silver nitrate (AgNO3) solution with ethyl acetate fractions (EAF) of Blumea lacera extracts. These bios conjugated HSNPs were then assessed for potential anti-inflammatory and antibacterial activities along with in vitro antioxidant effect. Methods and Results: The synthesis was confirmed by absorbance peak at 441 nm due to surface plasmon resonance in UV-visible spectrophotometer. FTIR spectra of HSNPs indicated the phytochemicals having C-O bond responsible for reducing of Ag+ to Ago. Average size of HSNPs was found to be 59.21 nm which was in good agreement with TEM and SEM results. EDS analysis showed the existence of Silver, Nitrogen and Carbon in HSNPs. The antibacterial activity of HSNPs in terms of zone of inhibition (ZOI) via disc diffusion assay and against Staphylococcus aureus and Escherichia coli was found to be 25.0±1.19 mm and 18.3±2.08 mm, respectively. The minimum inhibitory concentration (MIC) of HSNPs was found to be 50 μg/ml and 60 μg/ml against S. aureus and E. coli, respectively. The antioxidant capacity of the HSNPs was insignificant as compared to EAF but the results of anti-inflammatory activity was significant (p<0.05). Conclusion: The overall result demonstrated better in-vitro pharmacological potential of HSNPs compared to neat extract/EAF. Key words: Green synthesis, Phytopharmaceuticals, Inflammation, Kukrounda, HPTLC.
Keywords: Green synthesis; HPTLC.; Inflammation; Kukrounda; Phytopharmaceuticals

Development of Patient Safety Management Learning Model Based on Problem Based Learning Integrated Soft Skill Higher Level Thinking for Health Students in Samarinda - Hilda; Supriadi; Hesti Prawita Widiastuty; Arsyawina; Anwar Mallongi (Pharmacognosy Journal, 2023)
Background: Nursing students who participate in clinical practice in hospitals are not ready to provide quality care in maximizing patient safety. This study aims to develop a Problem Based Learning Patient Safety (PBLPS) conceptual model design that stimulates high-level thinking soft skills for students and finds PBLPS learning tools. Method: Research and Development (R&D), which is a research method that aims to produce new products through a development process. The stages of the research method consist of 3 stages, namely: the preliminary study stage; model development stage; model testing stage. The research subjects were nursing students at tertiary institutions in Samarinda city who had taken patient safety management courses, totaling 162 people. Data were analyzed using the Wilcoxon test. Results: Patient safety goals have not been implemented properly by students who practice clinical practice in hospitals. Students state that case-based learning can improve both hard skills and soft skills. The design of the integrated Problem Based Learning (PBL) learning model and the integrated PBLPS learning tools developed are RPS and integrated PBL-based modules on patient safety management. The results of the analysis of the soft skills assessment of high-level thinking students who practice in hospitals in the first year and the second-year show that there are differences in the results of the assessment of high-level thinking soft skills in the application of patient safety. Conclusion: The Problem Based Learning Patient Safety (PBLPS) conceptual model can stimulate high-order thinking soft skills for students in patient safety management practices in hospitals.
Keywords: Patient Safety; Problem Based Learning; Soft skills.

Diuretic Potency of Belalai Gajah Plants (Clinacanthus nutans (Burm.fil.) Lindau) - Ruqiah Ganda Putri Panjaitan; Afandi; Syarifah Ditha Aprilia (Pharmacognosy Journal, 2023)
Backgrounds: The use of belalai gajah plants (Clinacanthus nutans (Burm.fil.) Lindau) is believed by the Chinese community to be able to treat hypertension, so to prove whether this power exists in belalai gajah plants is to do a diuretic activity test. Objective of Study: to determine the presence of diuretic potency in belalai gajah plants. Methods: This test used male white rats of the Sprague Dawley strain with a body weight of 200-250 grams aged 2-2.5 months as many as 35 rats which were divided into 7 groups. The first group or no treatment, the second group with 2.8 ml/200 g body weight of distilled water, the third and fourth groups with 0.72 mg/200 g body weight of furosemide and 1.44 mg/200 g body weight, the fifth, sixth, and seventh groups with administration of belalai gajah plants extract doses of 75 mg/kg body weight, 150 mg/kg body weight, and 300 mg/kg body weight. This test uses the Cumming method. Urine collection was carried out for 24 hours, then urine volume, urine pH, also sodium and potassium levels in rat urine were measured. Results: The results of this study showed that without treatment, the administration of distilled water at a dose of 2.8 ml/200 g body weight, furosemide at a dose of 0.72 mg/200 g body weight and 1.44 mg/200 g body weight, and belalai gajah plants extract at a dose of 75 mg/kg body weight, 150 mg/kg body weight, and 300 mg/kg body weight resulted in a urine volume of 13.01 ± 0.07 ml; 13.77±0.17 ml; 15.78± 0.39 ml; 18.13±0.23 ml; 14.05±0.06 ml; 16.78±0.12 ml; 17.98±0.09 ml (p<0.5). Furthermore, the urine pH was successively 7.02 ± 0.04; 7.11±0.02; 7.21±0.02; 7.58±0.18; 7.15±0.03; 7.37±0.03; 7.44±0.02 (p>0.5). The results of sodium levels in urine were respectively 1.06±0.03 mEq/ ml; 1.14±0.03 mEq/ml; 2.17±0.09 mEq/ml; 4.01±0.069 mEq/ml; 1.34±0.08 mEq/ml; 1.49±0.02mEq/ml; 1.77 ±0.05 mEq/ml (p<0.5) and urinary potassium levels 0.5±0.03 mEq/ml, respectively; 0.57±0.018 mEq/ ml; 0.98±0.023 mEq/ml; 1.88±0.04 mEq/ml; 0.6±0.02 mEq/ml; 0.76±0.021 mEq/ml; 0.87±0.032 mEq/ ml (p<0.5). Conclusion: Belalai gajah plants extract at a dose of 300 mg/kg body weight has the ability to increase urine volume which is close to the urine volume by administering furosemide 1.44 mg/200 g body weight. Belalai gajah plants extract at a dose of 75 mg/kg body weight had a urine pH close to urine pH by administering distilled water at a dose of 2.8 ml/200 g body weight and 0.72 mg furosemide/200 g body weight. Giving belalai gajah plants extract at a dose of 75 mg/kg body weight has potassium levels in the urine that are close to urine potassium levels by administering distilled water.
Keywords: Clinacanthus nutans (Burm.fil.) Lindau; Diuretic Potency; Urine.

Diversity of Types of Medicinal Plants and Local Wisdom of the Kaili Tribe in Processing Medicinal Plants Around the Forest Areas of Central Sulawesi, Indonesia - Abdul Hapid; Ariyanti Ariyanti; Erniwati Erniwati; Ni Made Dharma Shantini Suena; Ketut Agus Adrianta; Karnita Yuniarti; Muthmainnah Muthmainnah (Pharmacognosy Journal, 2023)
The forest area in Sigi Regency, Central Sulawesi, is a habitat for various medicinal plant species. Forest areas must be managed appropriately for the local community and society's welfare and sustainable use. One of the local people living around the forest is the Kaili tribe. The local wisdom of the Kaili tribe, who inhabit forest areas in Central Sulawesi, in managing forests and forest products, especially using plants as medicine, must be documented in research. This study aimed to identify the species, habitat, habitus, and ways of using the plant as medicine by the Kaili people in the Sigi Regency. Prior to data collection, a literature study was conducted to obtain information regarding the condition of the research location around the forest area and the ethnic/ethnic groups who live permanently around and within the forest area. This stage was then followed by interviews and field surveys. The interviews were conducted by combining purposive sampling and snowball methods. The results showed the high potency of medicinal plants around forest areas used by the Kaili tribe, with the discovery of 113 species in 51 families. Herbs are the most common habitus of medicinal plants. The habitat of medicinal plants is mainly found in the yard. The most used plant part is the leaves, which can be boiled in water and drunk directly.
Keywords: Kaili tribe; Local knowledge; Medicinal plants; Species diversity.

Does Land-Use Associate with Escherichia coli Pollution in Karst Springs? A Review of the Literature - Ferdy Salamat; Anwar Daud; Anwar Mallongi (Pharmacognosy Journal, 2023)
Objective: The presence of Escherichia coli bacteria in karst springs, influenced by land cover conditions and land use activities in rainwater catchment areas, indicates a decrease in water quality. The study aimed to describe the effect of land use on Escherichia coli contamination in karst springs. Methods: This was a narrative review retrieving studies from Scopus and PubMed databases. The keywords used are pollution, Escherichia coli, land use, and karst springs. Of the 52 studies identified in the search phase, ten studies were eligible for inclusion in this review. Results: The concentration of Escherichia coli increases with increasing rainfall in all types of land use, such as forested land, mixed garden land, residential land, and grazing or livestock land. Factors that cause concentration Escherichia coli does not meet water quality standards due to feces from wild animals, feces-contaminated fertilization, construction of non-water impermeable septic tanks, and feces from livestock grazing. Conclusions: Repairing septic tanks and sewage in areas with high population density may reduce Escherichia coli contamination. Further research is needed to formulate strategies and policies for managing springs in the karst areas.
Keywords: Escherichia coli; Karst Springs; Land-Use; Pollution

Effect of Butionin-Sulfaximine and Fluphenazine as Trypanothione Inhibitory Drugs on Promastigotes and Axenic Amastigotes of Leishmania Peruviana and Leishmania Braziliensis - Jesús Rojas-Jaimes; Marco Mesía-Guevara; Alexander Murillo-Zenozain (Pharmacognosy Journal, 2023)
Background: Leishmaniasis is a disease caused by the Leishmania parasite, which is difficult to diagnose, causes disfigurement and is difficult to treat. Objectives: To determine the effect of Butionin-Sulfaximine (BSO) and Fluphenazine on trypomastigotes and axenic amastigotes of Leishmania peruviana and Leishmania braziliensis. Methods: A study was performed with Butionin-Sulfaximine (BSO), Fluphenazine, and Glucantime (positive control,) utilizing respective concentrations of 41.7 mg/ml, 4.17 mg/ml, and 50 mg/ml for twenty-four hours on axenic amastigotes. Results: A significant difference (*P < 0.05) was found between the negative control group, Fluphenazine, and BSO within both the axenic amastigotes of L. peruviana (5.5 X 105 / ml for the negative control, 0.15 X 105 / ml for Fluphenazine, and 0.7 X 105 / ml for BSO) and L. braziliensis (6.9 X 105/ml for the negative control, 0.18 X 105/ml for Fluphenazine, and 0.22 X 105/ml for BSO). Another significant difference (*P < 0.05) was found in the promastigotes of L. peruviana (5.9 X 105 / ml for the negative control, 0.66 X 105 / ml for Fluphenazine, and 3.1 X 105 / ml for BSO) and L. braziliensis (8.7 X 105/ml for the negative control and 5.68 X 105/ml for Fluphenazine). Conclusions: From this, we conclude Fluphenazine and BSO present promising antiparasitic effects against axenic amastigotes of L. peruviana and L. braziliensis in both pharmacological tests of the in vivo model and their potential future use.
Keywords: Axenic Amastigote; Butionin-Sulfaximine; Fluphenazine; Leishmania

The Effect of Cardiac Catheterization Intervention on The Nutritional Status of Children with Acyanotic Congenital Heart Disease - Muqiemuddin Salim; I Ketut Alit Utamayasa; Roedi Irawan; Irwanto; Azwin Mengindra Putera; Meity Ardiana (Pharmacognosy Journal, 2023)
Background: Intervention is required since malnutrition in children with acyanotic congenital heart disease (CHD) will affect growth, development, and quality of life. Research on the impact of cardiac catheterization on the nutritional health of children with acyanotic CHD is limited, particularly in Indonesia. This study aims to analyze the effect of cardiac catheterization on the nutritional status of children with acyanotic CHD. Methods: A cross-sectional study was conducted between January 2019 and December 2021. The participants of this study were children aged 1-60 months with acyanotic CHD who had undergone cardiac catheterization. We compare the nutritional status of participants in three observation stages, including pre-intervention of catheterization and the 3rd and 6th months post-cardiac catheterization. The parameters of nutritional status are determined according to the mean of Z-scores of weight-for-age (WAZ), length-for-age (LAZ), and weight-for-length (WLZ) of participants. Results: A total of children with acyanotic CHD who underwent catheterization and 49 children were eligible participants. The mean age of children with acyanotic CHD patients who underwent catheterization was 31.51 months, and 89% of them were 1-5 years. Fifty-three percent of participants who underwent cardiac catheterization were male. The most common acyanotic CHD defects were the Patent Ductus Arteriosus (PDA) among 55.1% of participants. Significant differences were found in WAZ, LAZ, and WLZ in the measurement of three observation stages (p<0.05). Conclusion: Interventional cardiac catheterization affects and enhances the nutritional status of children with acyanotic CHD and may be suggested as an initial therapy to further evaluate the disease.
Keywords: Acyanotic CHD; Children; Congenital heart disease; Interventional cardiac catheterization.; Nutritional status

The Effect of Channa striata Extract on Serum Albumin and High Sensitive C-Reactive Protein in End-Stage Renal Disease Patients: A Randomized Controlled Trial - Wachid Putranto; Septina Hestiningrum; Nur Ismi Mustika Febriani; Kusmardi Kusmardi; Ratih Tri Kusuma Dewi; Santy Ayu Puspita Perdhana; Nurhasan Agung Prabowo; Yeremia Suryo Pratama (Pharmacognosy Journal, 2023)
Background: Albumin is a marker of nutritioinal inflammation and mortality. Chronic inflammation, as indicated by the concentration of a proinflammatory cytokine, high sensitivity C-reactive protein (hs-CRP) was reported to be high in end-stage renal disease (ESRD) patients. Channa striata (CS) contains high protein that can increase albumin levels and has anti-inflammatory effects. This study was conducted to determine the effect of CS extract on serum albumin and hs-CRP on ESRD patients. Methods: This study is a randomized, double blind, placebo-controlled study in patients with ESRD on hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD). Subjects were randomized to either a CS or a placebo group and were given a three times daily dosage of 500 mg of CS extract or 500 mg maltodextrin, respectively for 21 days. Serum albumin and hs-CRP were measured at the baseline, and at the end of the study. Result: Forty subjects were randomized into the study with 20 in the Channa striata group and 20 in the placebo group, with HD and CAPD patient evenly distributed among the group. Significant increase in serum albumin levels (p<0,001) and significant decrease of hs-CRP (p<0,001) were observed in the treatment group compared to control group at the end of the study. At the end of the study, there was no significant difference between serum albumin, hs-CRP, and their gradient between HD and CAPD patients in the intervention group. Conclusion: CS extract results in higher serum albumin and lower hs-CRP levels compared to placebo in our population.
Keywords: Albumin; Channa striata; End-stage renal disease; hs-CRP; Supplementation.

The Effect of Combination between Green Tea Extract and Curcumin Extract from Mt. Lawu on BAX, Bcl-2 and Caspase-3 in Cisplatin-Induced Rat Models - Novi Primadewi; Harijono Kariosentono; Ari Probandari; Budiyanti Wiboworini (Pharmacognosy Journal, 2023)
Introduction: The study determines effect of Combination between Green Tea and Curcumin Extract from Mount Lawu on BAX, Bcl-2 and Caspase-3 in Cisplatin (Cis)-induced rat models. Methods: We treated four rats in each group and randomly distributed them into four groups: group C (−) was the negative control group with no treatment given, group C (+) was the positive control group given Cis only, group A1 was given green tea extract and curcumin extract combination after Cis, and group A2 was given Ginkgo biloba after Cis. Expression levels of BAX, Bcl-2, and Caspase-3 were assessed by ELISA. An ANOVA, a parametric test, was used if the data were normally distributed. If there were significant differences between the three groups regarding BAX, Bcl-2 and Caspase-3, a post hoc test was performed to determine the differences between treatments. Results: The results of the study show that combination between green tea and curcumin extract can increase Bcl-2 levels with an average value of 15.42 + 0.76 ng/mL, better than Ginkgo biloba extract with a value of 13.50 + 0.47 ng/mL, reduce BAX and Caspase-3 levels with a value of 6.57 + 0.38 ng/mL and 2.89 + 0.19 ng/mL, better than Ginkgo biloba with a value of 7.34 + 1.06 ng/mL and 3.86 + 0.34 ng/mL. Conclusion: This research shows that Combination between Green Tea and Curcumin Extract can increase Bcl-2 levels and reduce BAX and Caspase-3 in Cis rat models after fourteen days of treatment, better than Ginkgo biloba.
Keywords: Antiapoptotic; Antioxidant; Ototoxicity.

Effect of Combination of Soybean and Phaleria macrocarpa Ethanol Extract on IL6, TNFα, VEGF and Fibroblasts in Mice Exposed to UVB - Sumarawati T; Chodidjah; Dina Fatmawati (Pharmacognosy Journal, 2023)
UV exposure causes inflammation and the generation of reactive oxygen species, both of which contribute to skin aging. The purpose of this research was to determine how a combination of Phaleria macrocarpa extract and soybean extract affected the number of fibroblasts, VEGF, IL-6, and TNF alpha expression, and blood levels of IL-6 and TNF alpha in UV-B-exposed mice. In this study, mice were placed into four groups: one control group, three treatment groups, and a combination of Phaleria macrocarpa:soybeans at a 1:1 ratio (com group). The mice were euthanized on days 5 and 21 for histological preparations and then examined under a light microscope. Using an Olympus C-21 microscope with an Optilab Advances camera at 1000x magnification, the fibroblast was studied by counting the number of fibroblast cells per field of view. The immunohistochemical approach was performed to analyze the expression of VEGF, IL-6, and TNF- in skin tissue. The ELISA technique was used to quantify the levels of IL-6 and TNF-alpha. SPSS ver 21 was used to analyze the data. On days 5 and 21, the number of fibroblasts and expression of VEGF, IL-6, and TNF alpha were significantly higher in the combination group than in the control, Phaleria macrocarpa, and soybean treatment groups. However, there was no significant change in IL-6 and TNF alpha levels across groups on days 5 and 21 (p > 0.05). Finally, a 1:1 mixture of Phaleria macrocarpa and soybeans reduced the number of fibroblasts and the production of VEGF, IL-6, and TNF alpha on days 5 and 21, but not in serum levels.
Keywords: Inflammation; Skin wound; UV B radiation

The Effect of Drying Methods on Chemical Profiling of Zingiberaceae Herbs Production - Dinar Sari C. Wahyuni; Siti A. E. Astuti; Soerya Dewi Marliyana; Yudi Rinanto; Tiara Dewi S. Pratama; Ahmad Fauzi; Ari Satia Nugraha (Pharmacognosy Journal, 2023)
Background: Drying method is one of the important steps in post-harvesting in herbal production in order to prolong the shelf-life of herbal medicine and to ensure the good quality. Objective: This research aimed to profile and quantify the metabolites changing using metabolomic based on 1H-NMR Spectroscopy on Curcuma and Zingiber genus due to the drying process. Material and Methods: Five species of the Curcuma and Zingiber genus were Curcuma longa L., Curcuma aeruginosa Roxb., Curcuma xanthorrhiza Roxb., Zingiber officinale Roscoe. and Zingiber amaricans BI. The drying method applied were freeze drying and oven drying method at -1080C and 500C, respectively. Samples were extracted using buffer phosphate and deuterated methanol (3:7). The analysis was performed using 1H-NMR spectroscopy 400 MHz with TSP as an internal standard. The 1H-NMR spectra were analysed with multivariate (Principal Component Analysis). Results: The relative concentrations of sucrose and fructose at 5 samples in freeze drying method showed significant higher content than those in oven drying. On the other hands, glucose was mostly significant higher concentration in oven drying at 5 samples. Fructose and sucrose were significantly higher in freeze drying method than those in oven drying in Curcuma and Zingiber genera. Relative glucose content was higher in oven drying at Curcuma genus Conclusion: Therefore, oven drying method which applied heating at 500C is an appropriate method for herbal medicine production, especially in Curcuma and Zingiber genus, for maintaining the major secondary metabolites.
Keywords: Curcuma Genus; Freeze drying; Multivariate; Oven drying; Zingiber Genus.

The Effect of Elderly Hadrah Gymnastics on Muscle Strength and Scope of Motion of Lower Extremity Joints in Elderly with Osteoarthritis (Martapura River Region, South Kalimantan) - Bahrul Ilmi; Parellangi; Akhmad Rizani; Hammad; Anwar Mallongi; Sukri Palutturi (Pharmacognosy Journal, 2023)
Increased life expectancy (UHH) in Indonesia from 71.7 years in 2016 (Novianty, Nodia, 2018). And it is estimated to increase to 72.2 years in 2035 (BPS, 2016). The implication of increasing the number of elderly people with high UHH is expected to be healthy and productive elderly people and free from diseases and health problems, however, the results of a preliminary study in East Banjarmasin, the number of elderlies is 207 elderly (Posyandu, 2018). And almost 70% experience health problems such as osteoarthritis, so it is necessary to intervene in the form of Elderly Hadrah Gymnastics (SHL) which can increase muscle strength and scope of joint motion of the Elderly, so that the Elder can live healthy and free from osteoarthritis. The purpose of this study was to determine the effect of SHL on muscle strength and joint range of motion in elderly people with osteoarthritis in the Suburb area, Sungai Martapura Village, South Kalimantan Region. Quasi Experiment research design with The Non-Randomized Control Group Pretest Posttest Design approach, the population of all elderly in the Martapura River area with a total of 110 elderly (Pemko Dinkes, 2016). The research sample was the elderly living in the Martapura watershed who had osteoarthritis, non-random sampling, purposive sampling technique, with a total sample of 68 elderly consisting of the control and treatment groups, each of which was 34 participants. Both groups measured muscle strength and joint range of motion before and after treatment. Data analysis used Paired T-test for paired samples and Independent T-Test for unpaired samples with normal distribution. The results of research show There is an effect of SHL on the Strength of Pelvic and Knee Muscles of the Elderly as well as the effect of SHL on the Scope of Pelvic and Knee Joints in the Elderly. In the elderly who have osteoarthritis, they should do elderly Hadrah Gymnastics regularly at least 2 times a week.
Keywords: Elderly Hadrah Gymnastics; Joint Scope; Knee Muscles; Osteoarthritis; Pelvic Muscles

The Effect of Family-Based Empowerment in Preventing Overweight and Obesity in Elementary School Children in Kupang - Fransiskus Geroda Mado; Nurhaedar Jafar; Masyta Muis; Ida Leida Maria; Muhammad Syafar; Darmawansyah; Muhammad Alwi Arifin; Anwar Mallongi; Toto Sudargo; Agustina (Pharmacognosy Journal, 2023)
Background: The prevalence of overweight and obesity continues to increase in both developing and developed countries and occurs in all age groups. As well as being the biggest threat to public health, especially non-communicable diseases. Childhood is the most special period in life. It is almost certain that many health problems/illnesses experienced during this period will be carried on into adulthood. The causes of obesity in children are very complex; genetic, biological, psychosocial, behavioral and sociocultural factors. The impact of overweight and obesity on children is related to physical and psychological effects. Lifestyle modification which includes increasing knowledge, diet, physical activity, sedentary behavior, socio-cultural aspects is believed to be a strategy in preventing and controlling overweight and obesity. This study aims to examine the effect of family empowerment on the prevention of overweight and obesity in elementary school children. This research is a quasy experiment that is pre-test and posttest with control group design. Parallel design with one treatment group and one control group. The sample is determined by purposive random sampling. The research sample was overweight/obese children in elementary school grades 4, 5 and 6, totaling 112 people. Data were obtained through a structured questionnaire covering family abilities, knowledge, physical activity and nutritional intake. Nutritional status was measured after measuring body weight and height using digital scales. Eating patterns were measured using a food frequency questionnaire and a 24-hour recall formula. Results: The results showed that there were differences in family ability (p<0.016), knowledge (p<0.001), physical activity (p<0.015) and nutritional intake (p<0.000) in the treatment group and the control group after implementing the family empowerment intervention. Conclusion: Family-centered empowerment interventions through health education about overweight and obesity management have a positive impact on increasing family capacity, family knowledge, physical activity and changes in family eating patterns. It is necessary to implement lifestyle modifications in preventing and controlling overweight and obesity. Family-centered empowerment interventions through health education about overweight and obesity management have a positive impact on increasing family capacity, family knowledge, physical activity and changes in family eating patterns. It is necessary to implement lifestyle modifications in preventing and controlling overweight and obesity. Family-centered empowerment interventions through health education about overweight and obesity management have a positive impact on increasing family capacity, family knowledge, physical activity and changes in family eating patterns. It is necessary to implement lifestyle modifications in preventing and controlling overweight and obesity.
Keywords: Elementary School Children.; Empowerment; Family; Obesity; Overweigh

The Effect of Hyperbaric Oxygen Therapy (HBOT) on the Histopathological Lung Cells of Male White Wistar Rats Which are Intoxicated with Methanol - Djatiwidodo Edi Pratiknya; Risma; Ariyo Sakso Bintoro (Pharmacognosy Journal, 2023)
Methanol is a liquid that has the characteristics of being light, very easy to evaporate, colorless, flammable, toxic, and has a very distinctive odor like ethanol but lighter. The World Health Organization (WHO) globally states that 320,000 people die every year due to methanol poisoning in the age range of around 15 years to 29 years. Usually, symptoms of poisoning caused by methanol appear within 30 minutes to 2 hours. Based on the phenomenon that occurred, the author wanted to find out more about the effect of HBOT administration on the histopathological appearance of lung cells in mice intoxicated with methanol. This study used 30 male white wistar rat lung samples aged 2 to 3 months weighing around 150 grams to 250 grams, divided into three groups, namely the control group, treatment group I, and treatment group II. The control group was not given methanol treatment and hyperbaric oxygen therapy (HBOT). Treatment group I was given methanol for 14 days without hyperbaric oxygen therapy. Meanwhile, the treatment group was given methanol for 14 days and then given hyperbaric therapy for 10 days. The results show p<0.0006, which means the data is significant and there are differences between groups. The results of this study can be concluded that administering hyperbaric oxygen therapy to rats intoxicated with methanol had a statistically significant improvement effect on the alveolar membrane, alveolar lumen, and connections between the alveoli.
Keywords: Histopathological Lung Cells; Hyperbaric Oxygen Therapy; Methanol

Effect of Hypnoanesthesia on Endogenous Opioids (Beta Endorphin and Enkephalin) - Ihyan Amri; Abdul Hafid Bajamal; David S. Perdanakusuma (Pharmacognosy Journal, 2023)
Background: Hypnoanesthesia is a state of anesthesia achieved through hypnosis techniques. Meanwhile, hypnosis is a condition in which the mind receives information without analyzing it. Hypnoanesthesia has been empirically utilized in surgery since the 18th century, but the mechanism remains unclear. Objective: This study aims to prove nociceptive pain relief due to hypnoanesthesia in minor surgery, with indications of changes in the serum levels of several excitatory and inhibitory neurotransmitters in the physiological mechanism of pain. Methods: The study subjects included patients with benign soft tissue tumors consisting of 40 people who were divided into 2 groups (treatment and control). The treatment group underwent minor surgery using hypnoanesthesia, while the control group underwent minor surgery using 2% lidocaine local anesthesia. Pain in both groups was measured by FPS (Face Pain Scale) and monitored by a vital sign monitor. Changes in the serum levels of glutamic acid, substance P, beta-endorphin and enkephalin neurotransmitters in both groups before and after the intervention were analyzed using ELISA. Results: There were no significant changes in serum levels of neurotransmitters, pre and post intervention in both groups, both excitatory neurotransmitters Glutamic Acid and Substance P and inhibitory neurotransmitters Beta Endorphins and Enkephalin (p > 0.05). Conclusion: Based on the results of the data analysis, it can be concluded that the empirical nociceptive pain relief occurred in patients who underwent minor surgery with hypnoanesthesia and there was no significant change in betaendorphin, enkephalin, glutamate acid, and substance P serum levels before and after hypnoanesthesia
Keywords: Beta-Endorphin; Enkephalin; Glutamic Acid; Hypnoanesthesia; Substance P.

The Effect of Hypnoanesthesia on Serotonin - Ihyan Amri; Abdul Hafid Bajamal; David S. Perdanakusuma (Pharmacognosy Journal, 2023)
Background: Every minor and major surgical procedure requires anesthesia to relieve pain during surgery. The neuro-biomolecular mechanism of pain relief in hypnoanesthesia remains uncomprehended. Objective: This study aims to observe the effect of hypnoanesthesia on several neurotransmitters, including serotonin, glutamic acid, and substance P, which play a role in the mechanism of pain. Methods: The study subjects included patients with benign soft tissue tumors consisting of 40 people who were divided into two groups, namely treatment and control groups. Minor surgery with hypnoanesthesia was performed in the treatment group, whereas in the control group, minor surgery was performed with 2% lidocaine local anesthetic. Pain in both groups was measured by FPS (Face Pain Scale) and monitored by a vital sign monitor. The processes of the study were recorded with a camcorder. Changes in serum levels of excitatory (glutamic acid and substance P) and inhibitory (serotonin) neurotransmitters before and after the intervention were analyzed using ELISA (Enzym-Link Immunosorbent Assay) in both groups. Results: The control and treatment groups had the same end result, which was pain relief. The results of regression and ANOVA analysis indicated that serotonin simultaneously had a significant effect on substance P at 98.4% and glutamic acid at 98.2%. Conclusion: There was no statistically significant change in serotonin levels before and after hypnoanesthesia. Serotonin, as an inhibitory neurotransmitter, simultaneously has a significant effect on both excitatory neurotransmitters, namely Glutamate Acid and Substance P, in the mechanism of nociceptive pain relief with hypnosis.
Keywords: Glutamate Acid; Hypnoanesthesia; Serotonin; Substance P.

The Effect of Jelly Candy Snake Fruit and Banana With Ferrous Fumarat Fortified using Nano Technology in Adolescent Female at Junior High School - Meti Megawati; Toto Sudargo; And Susetyowati (Pharmacognosy Journal, 2023)
Aim: Adolescent Female are a population at risk for anemia. Nutritional factors such as calories, protein, iron, dietary fiber, and folic acid induce this disease. Furthermore, fishy odor and nausea are two factors that contribute to adolescent females’ lack of iron consumption. Jelly candy formulations of snake and banana fruit with ferrous fumarate fortification using nanotechnology also decrease these diseases as an alternative to food consumption. Young adolescents ought to embrace this jelly formulation, considering its reasonable pricing. Therefore, this study aims to determine the effect of jelly candy formulations of snake and banana fruit with ferrous fumarate fortification using nanotechnology on the increase in hemoglobin levels. Materials And Methods: A quasi-experimental approach was adopted with a Pretest-Posttest Control Group design. The study included a cohort of 150 adolescent girls hailing from Tamansari District, Tasikmalaya City, within the age range of 12 to 18 years. Additionally, the subjects were divided into three distinct groups, namely the treatments (involving jelly, snack fruit, banana, and ferrous fumarate supplementation, abbreviated as JEPISA), control group 1 (comprising jelly and ferrous fumarate supplementation, denoted as JFumarate), and control group 2 (receiving iron and folic acid supplementation as part of the Programme), each consisting of 50 participants. The sample selection process employed the purposive sampling technique and the collected data were subjected to analysis using T-Test, Chi-square and Multiple Logistic Regression methods. Results: The results of the T-Test analysis showed that the mean rise in hemoglobin after lean period of 13 week after giving treatments increased hemoglobin levels by 0.65 ± 0.39 g/dl (95% CI 0.53-0.75 g/dl), significantly (p-value=0,000). Their mean post intervention haemoglobin of three group was 12.10 ± 0.80 g/dl, 12.42 ± 0.70 g/dl and 11.73 ± 0.90 g/dl respectively. The results of statistical test were obtained, the treatment and control group 1 (p=0,044) and the treatment and control group 2 (p=0,33). The result of the multivariate analysis showed that complaince (p=0,000), nutritional status (p=0,000) and menstrual duration (p=0,000) variable were confounding variables for the incidence of anemia (Exp(B)=17,769). Conclusion: There were differences in Hb levels before and after administering the treatment. However, there was statistically significant difference between treatment with controls 1 and 2. Weekly supplementation of treatment’s in Iron Deficiency Anemia patients is as good as weekly supplementation of control 1 and but higher than control 2. Adolescent female who did not adhere to consuming iron had a chance of experiencing anemia 17,769 times compared to obedient adolescents after controlling for the variable nutritional status and length of menstruation.
Keywords: Adolescent female; Anemia; Fortified; Hemoglobin; Jelly Candy

Effect of Lactobacillus Plantarum IS-10506 on Paneth Cell Regeneration in the Ileum of Sprague Dawley Rats - Rasio Putra Hutama; Alpha Fardah Athiyyah; I.G.M. Reza Gunadi Ranuh; Andy Darma; Khadijah Rizky Sumitro; Wibi Riawan; Ingrid S. Surono; Subijanto Marto Sudarmo (Pharmacognosy Journal, 2023)
Background: Pathogenic Escherichia coli (E. coli) is the most common infectious agent among children in developing countries. Indigenous probiotics are not widely used to treat diarrhea and intestinal infections. This study aims to investigate the cell regeneration process of paneth cells after administration of Lactobacillus plantarum IS-10506 due to damage caused by Lipopolysaccharide (LPS) E. coli O55:B5, through the expression of MATH-1 and DEFA-6. Methods: This study used 64 paraffin blocks from Rattus norvegicus strain Sprague-Dawley divided into four groups. There were three treatments, KN, KL, KP and KPR groups, The KN group represent the administration of placebo. The KL group received LPS E. coli O55:B5 on day one. The KP group received LPS E. coli O55:B5 on the first day and Lactobacillus plantarum IS-10506 on the second day until six-day. The KPR group were administered Lactobacillus plantarum IS- 10506 six days prior to receiving LPS E. coli O55:B5, respectively. All groups, except KN, received LPS at a dose of 250 μg/kg body weight once, and Lactobacillus plantarum IS-10506 at a dose of 2.86x1010 CFU/ day. Evaluating paneth cell regeneration, DEFA-6, and MATH-1 expression immunohistochemistry was conducted on all tissues. Results: The expression of DEFA-6 and MATH-1 in the KP and KPR groups on day three of observation was significantly higher from the KL group. Even though the KL group achieved significant growth, the results of this expansion were significantly smaller than KP and KPR groups. Conclusion: After mucosal injury caused by LPS E. coli O55:B5, administration of probiotic Lactobacillus plantarum IS-10506 may increase paneth cell regeneration through differentiation and cell number.
Keywords: DEFA-6; Escherichia coli O55:B5; Lactobacillus plantarum IS-10506; Lipopolysaccharide; MATH-1.; Paneth cells

The Effect of Moringa Leaf Extract Intervention Since Preconception Period on the Prevention of Oxidative Stress in Pregnant Women and Adverse Pregnancy Outcomes - Rahma; Veni Hadju; A. Arsunan Arsin; Aminuddin Syam; Anwar Mallongi; Abd. Farid Lewa; Haerani Harun; Miranti (Pharmacognosy Journal, 2023)
Background: Oxidative stress occurs due to an imbalance of oxidants and anti-oxidants and is often associated with poor pregnancy outcomes. Therefore, additional supplementation is needed since the preconception period to prevent oxidative stress and poor pregnancy outcomes. This study aims to examine the effect of supplementing Moringa leaf extract on the prevention of oxidative stress in pregnant women and poor pregnancy outcomes. Methods: This study is an observational study with a prospective cohort study design. The research subjects were third trimester pregnant women consisting of 2 groups, namely the control group who only received Iron-Folic Acid (IFA) supplements (n = 30) and the group that received moringa leaf extract and IFA supplements (n = 26). Then an examination of Malondialdehyde (MDA) levels as a biomarker of oxidative stress was carried out using the ELISA (enzyme-link immune sorbent) method, followed by pregnancy outcomes including birth weight and birth length. Data were analyzed by unpaired t test. Results: The results showed that there were no significant differences in the levels of MDA, birth weight and birth length in the two groups with a p value > 0.05. Nevertheless, MDA level in IFA group was 49.25 nmol/ml higher than Moringa + IFA group which was 47.58 nmol/ml. Likewise, the average of birth weight and birth length in Moringa group were 3042.3 grams and 48.62 cm higher than IFA group which were 2993.7 grams and 48.23 cm. Malondialdehyde levels were also higher in women who had babies with birth weight < 2500 grams was 49.48 nmol/ml and birth length < 48 cm was 49.13 nmol/l compared to birth weight ≥ 2500 grams was 48.50 nmol/ml and birth length ≥ 48 cm was 48.62 nmol/ml but there was no significant difference in the two groups (p>0.05). Conclusion: Oxidative stress in pregnant women tends to be associated with poor pregnancy outcomes. Moringa leaf extract supplementation since preconception can prevent increased oxidative stress and improve pregnancy outcomes.
Keywords: Malondialdehyde; Moringa oleifera; Oxidative stress; Pregnancy outcomes.

Effect of Naphthaleneacetic Acid (NAA) and 6-Benzylamino Purine (BAP) on In-Vitro Propagation of "Mashua" (Tropaeolum tuberosum Ruíz & Pavón) Morphotypes from Peru - Gilmar Peña-Rojas; Luz Quispe-Calle; Vidalina Andía-Ayme; Alex Pereda-Medina; Rolando Estrada- Jimenez; Josefa Bertha Pari-Olarte; Elizabeth Julia Melgar-Merino; José Santiago Almeida-Galindo; Oscar Herrera-Calderon (Pharmacognosy Journal, 2023)
Background: Tropaeolum tuberosum Ruız & Pavón “mashua” is a native species of the central Andes and different propagation methods in vitro have been evaluated. However, this research has studied the in vitro propagation of 15 mashua (Tropaeolum tuberosum Ruíz & Pavón) morphotypes from the Ayacucho and Apurímac regions (Peru) Objective: To evaluate the effect of the additives naphthaleneacetic acid (NAA) and 6-benzylamino purine (BAP) on the micro-propagation rate of T. tuberosum. Material and Methods: For in vitro establishment, seedlings were used after disinfection with sodium hypochlorite, propagated in Murashige and Skoog (MS) medium. For the evaluation of the effect of NAA and BAP additives, nodes were isolated from in vitro seedlings after 30 days of cultivation and transferred to MS medium supplemented with 3% sucrose, pH 5.6 and 7 g/L of agar according to T1 (MS + NAA 1ppm) and T2 (MS + BAP 1ppm) treatments. The samples were cultivated for 28 days at 19 °C ± 2 °C with photoperiods of 16 h/light and 8 h/darkness. Results: After evaluating 20 repetitions for each of the three treatments, the addition of NAA or BAP does not improve the micropropagation rate of practically all the morphotypes studied. Furthermore, BAP behaved as an inhibitor of the development of mashua seedlings. Conclusion: The best micropropagation medium of Tropaeolum tuberosum Ruíz & Pavón (mashua) is the basic Murashige and Skoog (MS) medium without the NAA or BAP additives.
Keywords: BAP.; Mashua; Micropropagation; NAA; Tropaeolum tuberosum

Effect of Plant Stage and Solvent Extraction on Catechin Contents in Borrasus flabellifer L. Male Flower - Chayut Fongsuk; Patnaree Wongmanit; Kanoktip Pansuksan (Pharmacognosy Journal, 2023)
Borrasus flabellifer L. is a plant in the Arecaceae (Palmae) family with numerous bioactivities such as diuretic promotion, antioxidant, and antimicrobial effects. In this study, we are interested in the phytochemical compounds present in B. flabellifer L. male flowers. We investigated the effects of plant stage and solvent used for extraction. Initially, both the young and mature male flowers of this plant were extracted using water or ethanol. Several standard compounds, including gallic acid, catechin, caffeic acid, and quercetin, were utilized to screen the phytochemicals in these plant flowers. After analyzing the samples using High-Performance Liquid Chromatography (HPLC) method, only catechin was detected in all extracts, while the others were not detected (did not match the peak spectrum pattern compared with standard). Using a detection wavelength and curve parameters with an R2 value of 0.9999, catechin detection via HPLC was performed at 280 nm within the range of 13.12 to 210.00 µg/mL detection. Regarding the stage of the male flower, the young ones extracted with water provided the highest catechin content at 6.65 µg/mg extract, while the others fell within the range of 4.02 to 4.44 µg/mg extract. Therefore, it has been confirmed that catechin can be found in B. flabellifer L., and water should be used as the solvent for extraction for further applications of this plant.
Keywords: Borrasus flabellifer L.; Catechin; HPLC; Male flower; Mature; Young

Effect of Platelet Concentrates (PCs) Leucodepletion on the Activation and Efficacy of Platelet Transfusion - Teguh Triyono; Zelly Dia Rofinda (Pharmacognosy Journal, 2023)
Platelet concentrates (PCs) are blood components transfused in thrombocytopenic patients, including patients with blood malignancies. PCs contain leukocytes, which can pose potential side effects and activate platelets, reducing the efficacy of platelet transfusion. The leucodepletion process can be carried out by filtration to reduce the leukocyte count. This study aims to determine the difference between the CD62P expression of nonleucodepleted and leucodepleted PCs and to determine the difference between the Corrected Count Increment (CCI) of patients transfused with nonleucodepleted and leucodepleted PCs. This analytic observational study with a cross-sectional design was carried out on PCs obtained from platelet-rich plasma (PRP-PC). PCs were transfused into 48 blood malignancy patients, Yogyakarta, consisting of two groups i.e the group transfused with nonleucodepleted PCs (24 patients) and the group transfused with leucodepleted PCs (24 patients). CD62P expression in PCs was measured by flow cytometry method, and the CCI of the patients was calculated based on the CCI formula. The difference between the median CD62P expression and CCI of the two groups was analyzed using the Mann- Whitney Test with a significance of p<0.05. The median CD62P expression of the nonleucodepleted and leucodepleted groups were 34.4% (16.8–94.4%) and 21.7% (6.2–34.0%), respectively. There was a statistically significant difference between the CD62P expression of the two groups (p = 0.00). The group transfused with nonleucodepleted, and leucodepleted PCs showed respective median CCI of 18.8 x 109/L (2.4–94.8 x 109/L) and 14.7 x 109/L (2.4–124.0 x 109/L). There was no statistically significant difference between the CCI of the two groups (p = 0.42). It can be concluded that the CD62P expression in the PCs of the leucodepleted group was significantly lower than those of the nonleucodepleted group and that there was no significant difference between the CCI of both groups.
Keywords: CD62P; Corrected count increment; Platelet concentrates

The Effect of Preconcenption Moringa Leaf Extract Supplementation on Cotinine Serum Levels in Pregnant Women Exposed to Tobacco Smoke - Haerani Harun; Anwar Daud; Ridwan Amiruddin; M Sabir; Veni Hadju; Anwar Mallongi; Rahma; Miranti; Tamrin Talebe; M. Faris (Pharmacognosy Journal, 2023)
Background: Moringa oleifera is known for its nutrition content, antioxidant and medical benefit. Tobacco smoke contains various toxic substance and can affect the pregnancy. To reduce the tobacco smoke effect on pregnancy outcome good nutrition is needed. Objective: This study aims to assess the effect of Moringa leaf extract on serum cotinine levels in pregnant women exposed to second-hand tobacco smoke. Method: This is an observational study with a prospective cohort study design the study population was pregnant women which were divided by 2 groups, first group received IFA supplements only (n = 30) and the second group receive IFA and Moringa leaf extract supplements (n = 26). The tobacco smoke exposure data collected with questionnaire, and cotinine serum by blood sample. The tobacco smoke exposure and cotinine serum levels were compered between the two groups. Results: The tobacco smoke exposures at home were higher in MO+IFA group pregnant women then the IFA group pregnant women (p<0,05). The cotinine serum levels in both groups were found not significantly different (p=0,535). These results indicate that pregnant woman with high exposure of tobacco smoke who MO receive have similar cotinine serum levels with the pregnant woman with lower exposure of tobacco smoke. Conclusion: Moringa leaf extract supplementation to pregnant women with high tobacco smoke exposure has serum cotinine levels similar to pregnant women with lower exposure to tobacco smoke.
Keywords: Cotinine; Moringa oleifera.; Preconception; Tobacco smoke

The Effect of Ramania Leaves Extract Gel (Bouea macrophylla Griff) on the Number of Osteoblast (in vivo Study of Post Extraction in Wistar Rats (Rattus norvegicus)) - Irham Taufiqurrahman; Siti Hajar Norma Gupita; Beta Widya Oktiani; Isyana Erlita; Andi Zulkifli; Veni Hadju; Anwar Mallongi; Eko Suhartono (Pharmacognosy Journal, 2023)
Ramania leaves extract contains secondary metabolite compounds such as flavonoids, steroids, phenols and terpenoids which can be used as an alternative medicine for socket wound healing. Flavonoids have anti-inflammatory and antioxidant properties that can accelerate wound healing and can stimulate the formation of osteoblasts. Analyzing the differences in the number of osteoblasts in the negative control group, positive control given aloe vera gel 15% and ramania leaf extract gel 15% on day 5, 7 and 14. This study used true experimental design with posttest-only control design, using 27 rats divide into 3 groups. The experimental animals were sacrificed on days 5, 7 and 14 for histopathological observations. Two-Way ANOVA test results obtained p value = 0.013 <0.05, which indicates that there is a significant difference between the use of 15% ramania leaf extract gel 15% and the number of osteoblasts on days 5, 7 and 14. Bonferroni's Post Hoc test showed a difference. The number of osteoblasts was significant among the negative control group with ramania leaf extract gel 15%. Ramania leaf extract gel 15% was proven to be able to increase the number of osteoblasts and has the potential as an alternative medicine for socket healing after tooth extraction.
Keywords: Bouea macrophylla Griff; Flavonoids; Osteoblast; Ramania leaf extract gel; Secondary metabolite; Tooth extraction

Effect of Rosmarinus Officinalis Essential Oil On Anxiety, Depression, And Sleep Quality - Paul Alan Arkin Alvarado-García; Marilú Roxana Soto-Vásquez; Luis Enrique Rosales-Cerquin; Santiago M. Benites; Taniht Lisseth Cubas-Romero; Demetrio Rafael Jara-Aguilar; José Gilberto Gavidia-Valencia; Iris Melina Alfaro-Beltrán (Pharmacognosy Journal, 2023)
Introduction: The objective of this investigation was to evaluate the effect of Rosmarinus officinalis essential oil on anxiety, depression, and sleep quality. Methods: The essential oils were extracted by hydro-distillation using a modified Clevenger-type apparatus and the chemical composition was performed by Gas chromatography with flame ionization detection (GC-FID) and Gas chromatography mass spectrometry (GC–MS). Furthermore, a quasi-experimental study was conducted, where 81 participants were divided into two groups, comprising a waiting list control group and an experimental group treated with essential oils. Anxiety and depression indexes were evaluated using the Zung Self-Rating Anxiety Scale (SAS) and the Zung Self-Rating Depression Scale (SDS), and sleep quality was measured with the Pittsburgh Sleep Quality Inventory (PSQI). Results: Chemical analysis showed that 1,8 cineole (37.6%), α-pinene (19.9%), and linalool (15.8%) were the main components. Anxiety, depression, and sleep quality scores showed a decrease in the post-test study phase compared to the pre-test in the experimental group (p<0.05). A large size effect was found in the case of anxiety (d = 1.491; g= 1.490) with 1-β=0.996, while medium size effects were observed for depression (d = 0.581; g= 0.582) with 1-β=0.585; and sleep quality (d = 0.586; g = 0.588) with 1-β=0.638. Conclusion: Essential oils extracted from Rosmarinus officinalis are more effective in calming anxiety and show moderate changes in depression and sleep quality.
Keywords: Anxiety; Depression; Essential oils; Rosmarinus officinalis; Sleep quality

The Effect of Safety Climate on Safety Behavior with Safety Motivation as An Intervening Variable on Contractor Workers in PT X - Darius Tandiabang; Syamsiar S. Russeng; Lalu Muhammad Saleh; Atjo Wahyu; Yahya Thamrin; Anwar Mallongi (Pharmacognosy Journal, 2023)
Safety climate in the workplace and safety behaviour of workers need attention to be able to assess what factors are the causes of safe action for workers. This study aims to determine the effect of safety climate on safety behaviour with safety motivation as an intervening variable on contractor workers at PT X. The type of research is analytical observational with a cross sectional design. Questionnaires were given to 380 respondents. The data were analyzed using path analysis to see the direct and indirect effects of safety climate on safety behaviour. The results showed, 361 respondents (95%) with good safety climate, 367 respondents (96.9%) with good safety motivation, and 348 respondents (91%) with good safety behaviour. The path analysis showed that there was no direct influence of safety climate on safety behaviour in contractor workers at PT X (p value >0.05). However, the safety climate indirectly affects safety behaviour through safety motivation (p value = 0.001). It is recommended to workers that they need to maintain and increase their motivation so the safe work behaviour will still create.
Keywords: Contractor workers; Path analysis.; Safety behaviour; Safety climate; Safety motivation

The Effect of Sapodilla Leaf Extract (Manilkara zapota L.) on Lipid Profiles of Alloxan-Induced Diabetic Mice - Tridiganita Intan Solikhah; Tania Anggreani Wijaya; Salsabila; Daffa Alice Pavita; Ragil Kusnandar Miftakhurrozaq; Hartanto Mulyo Raharjo; Maya Nurwartanti Yunita; Faisal Fikri (Pharmacognosy Journal, 2023)
The aim of this study is to examine the effect of sapodilla leaf extract on lipid profiles of alloxan-induced diabetic mice. This research method are 30 male mice were used as experimental animals, which were randomly divided into five groups, each group consisting of 6 mice. The division of the group is as follows: Treatment of non-diabetic mice, diabetic mice, diabetic mice by administering pioglitazone at a dose of 2 mg/kg BW, extracts of manila sapodilla leaf (M. zapota L.) dose 100 mg/kg BW and 300 mg/kg BW. Observations were made on the 14th day, after administration of sapodilla leaf extract, mice were given light anesthesia and serum lipid profiles Total Cholesterol (TC), triglycerides (TG), High-Density Lipoprotein (HDL), and Low-Density Lipoprotein (LDL) were measured using diagnostic kits (Pars Azmoon Kit) and automatic analyzer (Abbot, model Alcyon 300). The results of this study are in TC, there was a significant difference in pioglitazone and 100 mg leaf extract against all treatment groups. In TG, there was a significant difference in pioglitazone and 300 mg leaf extract against all treatment groups. In LDL, there were significant differences in the 100 mg, 300 mg leaf extract and pioglitazone, against negative control, and diabetes control. In HDL, there was a significant difference in negative control and 300 mg leaf extract, against 100 mg leaf extract, pioglitazone, and diabetes control. The conclusions of this study exhibited the ethanol extract of M. zapota leaves contains several phytochemical compounds including alkaloids, flavonoids, saponins, polyphenols, tannins, quinones, and steroids. M. zapota leaves extract (100 mg/kg and 300 mg/kg) exhibited a significant effect on improvement in lipid protein.
Keywords: Alloxan; Diabetes; Lipid Protein; Manilkara zapota

The Effect of Service Quality and Patient Satisfaction Toward Patient Loyalty in Special Regional Hospitals of South Sulawesi - Rara Armita Arman; Syahrir A Pasinringi; Fridawaty Rivai; Andi Indahwaty Sidin; Irwandy; Lalu Muhammad Saleh; Anwar Mallongi (Pharmacognosy Journal, 2023)
The improvement and guarantee of hospital Service Quality in Indonesia should always be in the best interest of any hospital as regulated in Article 40 of Hospital Law No. 44 of 2009. Adequate Service Quality in a hospital would result in emotional bonds and positive experiences felt by patients and family, eventually strengthening loyalty in the long run. Thus, Service Quality is a critical aspect and indicator to achieve patient satisfaction hence behavioral intention to build loyalty towards the hospital and future visits. The aim of this study is to identify the effect of Service Quality and patient satisfaction in relation to patient loyalty in Special Regional Hospitals of South Sulawesi. This is a quantitative study with a crosssectional model. The sample in this study were all patients who had been registered in the database, namely patients who had visited the outpatient service at Dadi Special Regional Hospital (RSKD), Siti Fatimah Regional Special Hospital for Mother and Child (RSKDIA), and Pertiwi Special Regional Hospital for Mother and Child (RSKDIA) in 2017-2019 with a total sample of 269 from the outpatient care, respectively. The inclusion criteria in this study are as follows: (1) Patients from the outpatient care. (2) Patients who had received hospital services twice. (3) Not suffering from any mental illness and sensory disturbances. (4) Patients who are willing to be respondents and consented to fill out research questionnaires. (5) The patient is conscious and is able to communicate. The results that were obtained show that Service Quality holds a significant effect on patient satisfaction. Service Quality has no significant effect on patient loyalty, while patient satisfaction has a significant effect on patient loyalty. The output of this study should be taken into consideration for hospitals to commit to improving the Service Quality in order to achieve patient satisfaction. Increased patient satisfaction in turn will develop loyalty, thereby accommodating the implementation of the hospital strategy and achieving hospital goals.
Keywords: Patient Loyalty; Patient Satisfaction; Regional Special Hospital.; Service Quality

The effect of Sinensetin and Imperatorin on A-549 lung cancer cell viability in vitro - Raden Anita Indriyanti; Eko Fuji Ariyanto; Hermin Aminah Usman; Ristaniah Rose Effendy; Diah Dhianawaty (Pharmacognosy Journal, 2023)
Introduction: Lung cancer remains the leading cause of cancer death worldwide, so research is ongoing to discover new therapeutics, such as plant-derived bioactive compounds. For example, Sinensetin, a plant-derived polymethoxylated flavonoid, and Imperatorin, a natural furanocoumarin, have anti-cancer properties. This study assessed the effects of sinensetin and imperatorin separately and in combination on A-549 lung cancer cell viability. Method: The A-549 lung cancer cell line was treated with sinensetin (60 μM), imperatorin (30 M), or a combination of both compounds (Sin:Imp 30:30 μM; 50:50 μM and 60:30 μM) for 48 hours. Cell viability was then assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and apoptosis was determined using fluorescein isothiocyanate (FITC) Annexin-V/Propidium iodide staining. Results: The combination treatment of Sin:Imp 50:50 and Sin:Imp 60:30 μM reduced cell viability more than the individual treatment of sinensetin and imperatorin, with the lowest cell viability observed for the combination treatment of Sin:Imp 50:50 μM. Likewise, the combination treatment of Sin:Imp 50:50 μM induced the most apoptosis compared to individual treatment. Conclusion: Sinensetin and imperatorin can decrease A-549 lung cancer cell viability and are potent apoptotic inducers, especially when they are used in combination, therefore they are potential lung cancer therapeutics.
Keywords: A549; Apoptosis; Cell viability; Imperatorin; Sinensetin

Effect of Single-Bulb Garlic (Allium sativum Var. Solo Garlic) Extract on The Hematological Profile In E-Cigarette-Induced Male Sprague Dawley Rats - Maya Nurwartanti Yunita; Julia Calvina Fauzi; Zahra Defitra Rahmania; Bella Safinda; Tridiganita Intan Sholikhah; Bodhi Agustono; M. Gandul Atik Yuliani; Shekhar Chhetri; Reina Puspita Rahmaniar (Pharmacognosy Journal, 2023)
Garlic and its derivative preparations have long been used in traditional medicine for good health. Garlic is known to act as an antioxidant, antitumor, antibacterial, and antifungal. The antioxidant compound in singlebulb garlic is considered to be better than the antioxidant content in other species of garlic. This research aimed to study the effect of hematological profile in oral administration of single-bulb garlic extract in e-cigarette-induced male Sprague Dawley rats. The method used in this study was randomized post-test only control group design. Twenty-four rats were randomly distributed into six groups. The group consisted of C (-) as normal rats’ control; C (+) only exposed to e-cigarette smoke; T1 exposed to e-cigarette smoke and treated with vitamin C; T2 exposed to e-cigarette and treated with single-bulb garlic extract 75 mg/kg BW; T3 exposed to e-cigarette and treated with single-bulb garlic extract 100 mg/kg BW; and T4 exposed to e-cigarette and treated with single-bulb garlic extract 125 mg/kg BW. Exposure to e-cigarette smoke is conducted by evaporating the liquid into a gas chamber with a nicotine dose of 3.6 mg/ml. All treatments were given for 14 days. On the 15th day, the rats were sacrificed. Blood samples were collected by the cardiac puncture method and were examined using a hematology analyzer. Data obtained were white blood count (WBC), platelets, hemoglobin, hematocrit, and erythrocytes that were analyzed with one-way ANOVA. The result of this study is that garlic extract may boost immunity in the body. However, higher doses can be toxic to erythrocytes.
Keywords: Antioxidant; E-cigarette; Good health; Hematology profile

The Effect of Syzygium Polyanthum (Wight) Walp. Extract on Glutathione, Tumour Necrosis Factor-Alpha, Spdl1, And Degraff Follicles Expression in PCOS Rat Models - Renny Aditya; Budi Santoso; Widjiati (Pharmacognosy Journal, 2023)
Background: Polycystic ovary syndrome (PCOS) is an endocrine and reproductive disorder affecting 5% to 13% of women of reproductive age. Approximately 80% of women with PCOS underwent infertility cases. Many studies have shown an association between PCOS and low-grade inflammation. Syzygium polyanthum (S. polyanthum) contains antioxidants and has antiinflammation activity. Objectives: This study aims to investigate the effectiveness of S. polyanthum leaf on glutathione, tumour necrosis factoralpha (TNF-α), sPDL1, and DeGraff follicle expression in PCOS rat models. Materials and Methods: The female Wistar rats were divided into five groups (n = 5), K0 (normal control), K1 (PCOS group), and three treatment groups which received three different doses of S. polyanthum extract. The treatment group consisted of PCOS rat models with S. polyanthum extract supplementation of 150 mg/KgBW (P1), 300 mg/KgBW (P2), and 450 mg/KgBW (P3). Results: S. polyanthum leaf extract significantly decreased TNF-α expression in the treatment group. The highest TNF-α, GSH, and sPDL1 concentration was found in the P3 group (3.83±0.123, 273.12±12.4 ng/ml, and 3.25 ng/ml, respectively). Moreover, the number of follicles was statistically significant in the P1, P2, and P3 groups (2.0, 3.0, and 3.50, respectively). The number of follicles in the P1, P2, and P3 groups was higher than in the positive control; however, it was lower in the negative control (K0) (p<0.05). Conclusion: S. polyanthum extract was significantly effective in decreasing TNF-α expression, as well as a significant increase GSH, sPDL1 and DeGraff foliclle count in PCOS rat model.
Keywords: DeGraff follicle count; GSH; sPDL1; Syzygium polyanthum; TNF-.

Effect of Thiamine on Serum Glutamate in Ischemic Stroke Animal Model - Mohammad Saiful Ardhi; Muhammad Hamdan; Achmad Chusnu Romdhoni (Pharmacognosy Journal, 2023)
Background: Thiamin or vitamin b1 is a therapy that has been widely used in neurology cases such as alcohol-induced encephalopathy and neuropathy. Thiamin has the ability to penetrate the blood brain barrier so that it is expected to be used as an additional therapy in stroke cases. Stroke is currently an emergency neurology case that causes high mortality and disability. Among the causes is because damage to large areas of the brain will cause the release of neurotransmitters such as glutamate which has excitotoxicity effects to the brain when excessive. Method: This study used animal models of carotid artery occlusion stroke. Divided into 4 groups: control, thiamin dose of 100mg/kg, Thiamin 200mg/kg, and 400mg/kg. Serum glutamate was collected on day 7 post-action. Then evaluated by ELISA method. Results: There was a significant difference in the form of a decrease in the average serum glutamate level between the control and the sample that received a dose of thiamine 200mg/kg and 400mg/kg. Conclusion: High-dose thiamine may be considered as an additional therapy in cases of carotid artery occlusion model stroke.
Keywords: Animal model; Glutamate; Ischemic; Stroke.; Thiamine

The Effect of Zamzam Water and ProShieldR Varnish on Microhardness of Eroded Primary Enamel Teeth Induced by Ironplus Syrup: An In Vitro Study - Isra’a JA. Altaee; Aisha A. Qasim (Pharmacognosy Journal, 2023)
Background: The objective of the current study was to evaluate the erosive adverse effect of paediatric Ironplus syrup and the remineralization effectualness of Zamzam Water and ProShieldR Varnish on microhardness of primary teeth. Materials and method: Forty primary anterior teeth were randomly distributed into five groups, each group has eight samples (N=8) assigned as: group1: control negative, group2: control positive, group3: 5% Sodium Fluoride Varnish, group4: Zamzam water and group5: Zamzam water and 5% Sodium Fluoride Varnish. Excluding the control negative group from them, all samples were immersed in 100ml of Ironplus syrup and agitated for 2 min once a day for 14 days. Then those groups were treated with remineralizing agents of: 5% Sodium Fluoride Varnish for 24 hours, Zamzam water for 30 min every 12 h for 12 days and the combination therapy initiated by Zamzam water then after 30 min, 5% Sodium Fluoride Varnish was applied for 24hrs. Vickers Microhardness test and Polarized Light Microscope were two adopted tests that this study dependent upon. Results: There was a statistically significant difference in microhardness mean values when making a comparison between the baseline and Ironplus tested groups, furthermore ANOVA and Duncan’s Multiple Range test showed that there was a highly significant statistical difference in microhardness mean values among baseline, demineralization and remineralization groups at P-Value ≤ 0.01. Conclusion: The surface microhardness of the primary enamel was reduced owing to the potential erosive efficacy of the used paediatric pharmaceutical preparations. On the other extreme, the previous readings had increased after being treated with remineralizing agents meaning that they have the capacity to battle against erosive potential reaching its highest capability in terms of reminerlization with the combination treatment group.
Keywords: Erosion; Ironplus Syrup; Microhardness; Polarized Light Microscope; ProShieldR Varnish; Zamzam water

The Effect Supplementation of Microbiota Inoculant in the Early Laying Hens Feed on High Density Lipoprotein (HDL) and Low- Density Lipoprotein (LDL) in Egg Yolk - Bodhi Agustono; Rini Apriliawati; Sunaryo Hadi Warsito; Maya Nurwartanti Yunita; Widya Paramita Lokapirnasari; Sri Hidanah; Emy Koestanti Sabdoningrum; Mohammad Anam Al-Arif; Mirni Lamid; Gandul Atik Yuliani; Shekhar Chhetri; Sarasati Windria (Pharmacognosy Journal, 2023)
Laying hens (Gallus gallus) are one of the poultries kept for their eggs. Eggs produced by chickens are used as a source of human food that contains a lot of nutrition and is affordable. Eggs also contain high levels of cholesterol in the yolk, so if consumed in large quantities, they will pose a risk of disease. This study aims to determine the effect of giving probiotics in the early layer laying hen feed on the levels of high-density lipoprotein and low-density lipoprotein in egg yolks. This study has four treatments, namely P1 = control, P2 = feed + AGP, and P3 and P4 = feed + probiotics. The probiotic used is a combination of three bacteria, namely Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium sp. The sample used in this study was egg yolk, which was collected in the last week of the study. Testing for HDL and LDL levels in egg yolks was carried out using the Quantitative Colorimetric/Fluorometric Determination of HDL and LDL/VLDL method. Testing HDL and LDL levels in this study showed that the administration of probiotics had an effect in the form of increasing HDL levels but did not have an effect in the form of a significant decrease in egg yolk LDL levels during the four-week treatment period in laying hens in the early layer phase, aged 18–22 weeks. The best probiotic dose used among the three probiotic doses is 3 ml/kg of feed.
Keywords: High Density Lipoprotein; Laying hens; Low Density Lipoprotein; Probiotics.

Effectiveness of Ketapang (Terminalia cattapa L.) Extract Against Avian Pathogenic Eschericia coli (APEC) Infections in Layer Performance - Kadek Rachmawati; Rochmah Kurnijasanti; Emy Koestanti Sabdoningrum; Sin War Naw (Pharmacognosy Journal, 2023)
This study aimed to determine the activity of Ketapang extract (Terminalia cattapa L.) in layers infected with APEC. In vitro study that was conducted dilution methods using Ketapang extract at 5%, 10%, 15%, 20%, 25% and 30% concentrations. In vivo study was conducted by randomly dividing 20 layers into five treatment groups, four per group. The chickens (except in group P0−) were infected with APEC aged 24 weeks. Then, the treatment was conducted according to the divided groups in one week. The said treatments are P0− (uninfected APEC and unadministered with Ketapang extract), P0+ (infected with APEC and unadministered with Ketapang extract), and P1, P2, and P3 (infected with APEC and administered with Ketapang extract with 5%, 10%, and 20% concentrations, respectively). The data from layers’ performance were analyzed using ANOVA and Duncan’s test. The dilution method (MIC and MBC) exhibited the antibacterial ability of Ketapang extract against APEC at 5% dose. The Ketapang extract administration in layers exhibited improved performance of chickens infected with APEC, with the administration of 10% dose of Ketapang extract showing the best result. In summary, about 10% concentration Ketapang extract can serve as an antibacterial agent and showed the best results in layers infected with APEC.
Keywords: Avian Pathogenic Escherichia coli.; Food security; Layer chicken performance; Terminalia cattapa L.

The Effectiveness of SI-DINI Mobile App (an Education and Early Detection of Leprosy App) on Changes in Community Behavior - Maspa Lapui; Wahiduddin; Ida Leida Maria; A Arsunan Arsin; Ridwan M Thaha; Syamsuar Manyullei; Anwar Mallongi (Pharmacognosy Journal, 2023)
Most leprosy case detection in Indonesia is based on passive surveillance. This condition impacts the high number of new cases of leprosy and the disability rate of leprosy. Data from the Ministry of Health of the Republic of Indonesia stated that for 2021, there were 7,201 new cases of leprosy with a disability proportion of 84.6%. The community's reluctance to make early contact is due to low knowledge and fear of stigma. SI-DINI is an android-based application that contains information about leprosy, videos on identifying the characteristics and symptoms of leprosy, and reporting suspected leprosy so that it is hoped that it can change people's behavior in a positive direction. This study aims to see the effectiveness of the SI-DINI App in changing people's behavior. This study used a quasi-experimental design with a pre and post-test design with intervention and control groups. The use of the SI-DINI App has been proven effective in changing people's behavior in terms of knowledge, attitudes and actions. There is a significant increase in the knowledge aspect with p-value = 0.000 (p<0,05), the attitude aspect p-value = 0.000 (p<0,05), and the action aspect p-value = 0.000 (p<0,05). It is hoped that the application of education and surveillance for early detection of leprosy (SI-DINI) can be applied on a broader scope and can be used sustainably and in the future.
Keywords: Attitude; Behavior.; knowledge; Leprosy; SI-DINI App

Effectiveness of the Aedes aegypti Mosquito Vector Control Program in Southeast Asia – A Systematic Review - Sulistiawati; Miranda Jemyma Mas’ulun; Anggita Kanza Ramadhany; Afiel Nadhifa Hanafie; Rani Fitri Alfiani; Sarah Evita Husnah; Agnes Ilene Suprapto Puteri; Anisa Novia Mahestari (Pharmacognosy Journal, 2023)
Background: Dengue is still a major vector-borne disease problem in the world. It is caused by infection with the Dengue virus, which can be spread through a vector in the form of the Aedes aegypti mosquito. One of the ways to reduce the incidence of dengue infection is by controlling vectors. Aim: This study aims to analyze the effectiveness of vector control of the Aedes aegypti mosquito on the dengue incidence in Southeast Asia. Methods and findings: This study was a systematic review that examines articles with experimental research designs and cluster randomized controlled trials. The scope of this research area is limited to Southeast Asian countries during 2012-2021. There are 333 articles obtained from two sources: PubMed and ScienceDirect, by using certain keywords. Furthermore, articles were selected based on inclusion and exclusion criteria, so that 31 articles were obtained. A total of 6 articles that met the inclusion criteria were then selected for further analysis. Three journals that discussed the output in the form of the Larva Density Index (LDI) showed a decrease with significant results. Two journals that discussed biological control (Bacillus thuringiensis israelensis) got significant results in decreasing Pupal Density (PD). The two journals on chemical control (permethrin) that discussed the outcome of the presence or absence of IgG seroconversion show insignificant results. Conclusion: All control methods are considered effective. However, among the three vector control methods studied, the control with Bacillus thuringiensis israelensis is the most effective method.
Keywords: Aedes aegypti; Dengue; Dengue Control Program; human and health; Medicine; Tropical Disease.

The Effectiveness of the Si-DBD Application in Increasing the Number of Households in Conducting Periodic Larvae Examination at the Health Center in Bantaeng Regency - Muhammad Firdaus; Ida Leida Maria; Ansariadi; Andi Zulkifli; Nurhaedar Jafar; Shanti Riskiyani; Anwar Mallongi (Pharmacognosy Journal, 2023)
Background: Vector-borne diseases, namely DHF, are estimated to infect 100-400 million people each year. Until now there is no specific drug or vaccine for DHF that can be done to avoid or prevent mosquitoes, which focuses on prevention efforts by eradicating mosquito nests. This study aims to see the effectiveness of the application system in carrying out periodic inspections by households. Method: Quasi-experimental research type with design "The Nonrandomized Control Group Pretest Posttest Design”. There are 112 samples with a ratio of 1:1 treatment (application system) and control (manual). Data collection using observation sheets (secondary data) and data from larvae inspection results through the application. Seeing the difference before and after the Mc test. Nemar and Chi-Square to see the difference between the treatment and control groups. Results: Periodic larvae inspection by the household before and after manually had no difference, while the system with the application had a significant difference with a value of P=0.000. The comparison between the treatment and control groups with a value of P = 0.000 indicates that there is a difference in the number of households that carry out periodic larva inspections through an application compared to the manual method. The system with the Si-DBD application is simpler, more acceptable, and timelier in carrying out periodic larvae inspections by households with a value of P=0.000. Conclusion: The implementation of periodic larvae inspections that is modeled by an application-based information system via a smartphone has an influence on the number of households that carry out larvae inspections compared to the manual system that is running. Follow-up testing can be carried out on a large scale of household respondents covering the entire work area of the health center and with a cohort research design.
Keywords: Application; DHF.; Periodic larva inspection

Effectivity of Patch Herbal Mixture Composed of Mangosteen Peel Extract and Bacterial Cellulose for Wound Healing - Marisca Evalina Gondokesumo; Yulanda Antonius; Yuana Elly Agustin (Pharmacognosy Journal, 2023)
Indonesia has a very abundant production of mangosteen, which places at the 14th largest mangosteen production in the world. High mangosteen production affects the amount of mangosteen peel waste. On the other hand, mangosteen peel has been identified as a rich source in health benefits and has proven to be antibacterial, antifungal, antioxidant, anti-inflammatory, antitumor, and anti-carcinogenic. Traditionally, one of the conventional medicines used in wound dressings or infection medicine. It can be a possibility for patch wound healing development, especially for diabetic ulcers with an open wound, wetness, chronic infections, and high inflammation. This research aims to innovate wound dressings (patch) that contain active compounds from mangosteen peel as herbs with many active compounds to enhance the wound healing process with low toxic side effects. The patch developed from organic material from bacterial cellulose. The research was carried out with the initial process of making patches from bacterial cellulose (BC), then adding mangosteen peel extract as patch dressing with different concentrations (0%, 1%, 2%, 5%, and 10% v/v). Furthermore, the patch was applied to the injured Wistar rat, for 15 days. The wound area was measured using Image-J. The results of measuring the wound area showed significant wound improvement every day at each dose of the mangosteen patch compared with the control group. The optimum dose that appears to be different from the control is 10% v/v mangosteen peel extract which has been able to leave 3% of the wound area on the 15th day.
Keywords: Bacterial cellulose; Herbal compound; Mangosteen peel extract; Patch dressing; Wound treatment.

Effects of Magnesium Supplementation on Kidney Function and Phosphate Levels in Children with Chronic Kidney Disease and Hyperphosphatemia: A Double-blind Randomized Clinical Trial - Glabela Christiana Pandango; Bambang Purwanto; Risky Vitria Prasetyo (Pharmacognosy Journal, 2023)
Background: Pediatric patients with chronic kidney disease (CKD) who have hyperphosphatemia may experience further deterioration in kidney function. This study aims to investigate the effect of magnesium supplementation on the reduction of phosphate levels and improvement of kidney function in children with CKD and hyperphosphatemia, compared to a placebo. Methods: A randomized, double-blind, placebocontrolled trial was conducted at Pediatric Ward in our setting during March-July 2022. We compared oral magnesium supplementation (6 mg/kg body weight/day for two months) with a placebo in children with CKD and hyperphosphatemia (ages 1-18 years old). Patients who were on dialysis and had serum magnesium levels of <1.6 mg/dL and >2.4 mg/dL, and were allergic to magnesium supplementation were excluded. A paired T-test and the Wilcoxon signed-rank test were used for statistical analysis. Results: We collected 31 children in the experimental group and 29 children in the placebo group. Phosphate levels were decreased in both the magnesium supplementation and placebo groups (5.4 ± 0.9 to 4.8 ± 1.1 mg/dL; p-value = 0.001 and 5.1 ± 0.6 to 4.3 ± 1.2 mg/dL; p-value=0.003). However, when compared between groups, the reductions were not significantly different (0.7 vs 0.8; p-value=0.935). A significant improvement was found in kidney function in both groups using estimated Glomerular Filtration Rate (eGFR) (83.4±25.3 to 118.8±52; p-value=<0.001 and 86.3±28.1 to 96.9 ± 35.8; p-value=0.004), and the reductions were significantly different (35.4 vs 10.7; p-value=0.045). Conclusion: Magnesium supplements have considerably lower phosphate levels and markedly improved kidney function in children with CKD and hyperphosphatemia.
Keywords: Children; Chronic Kidney Disease; Hyperphosphatemia; Kidney Function.; Magnesium

Effects of Melissa officinalis essential oil on state and trait anxiety - Paul Alan Arkin Alvarado-García; Marilú Roxana Soto-Vásquez; Francisco Mercedes Infantes Gomez; Natalia Mavila Guzmán Rodríguez; Yolanda Elizabeth Rodríguez de Guzmán; Demetrio Rafael Jara-Aguilar; Roger Antonio Rengifo-Penadillos; José Gilberto Gavidia-Valencia; Iris Melina Alfaro-Beltrán (Pharmacognosy Journal, 2023)
Introduction: Essential oils are complex substances used extensively in aromatherapy and phytotherapy, some of them as anxiety and stress-relieving agents. In order to evaluate the effects of Melissa officinalis essential oil on state and trait anxiety, this study was conducted. Methods: The essential oil was extracted by steam distillation and the chemical composition was investigated by Gas chromatographymass spectrometry (GC–MS). In addition, a Quasi experimental study with measures at pretest-posttest was conducted, where 58 participants were divided into two groups, a waiting-list (WL) control group, and an experimental group (EG)treated with aromatherapy based on Melissa officinalis essential oil. The anxiety index was evaluated by State-Trait Anxiety Inventory (STAI). Measures were taken at two times: pretest and posttest. Results: The chemical analysis showed that β-cubebene (26.3%), β-caryophyllene (25.4%) and geranial (12.5%) were de main components. Both state and trait anxiety levels decreased in the experimental group during the posttest phase; however, only state anxiety demonstrated statistically significant differences (p<0.05). A moderate size effect was found for state anxiety (d = 0.799; g= 0.797) with 1-β=0.803; but none for trait anxiety. Conclusion: Melissa officinalis essential oil was moderately effective on state anxiety but has no effect on trait anxiety.
Keywords: Essential oil; Melissa officinalis.; State anxiety; Trait anxiety

Elucidating the Use of the Plant Paeonia Anomala from the Ancient Medical Books Kept in Mongolia - Ariunjargal Tudev; Sansarkhuyag Enkhtur; Khurelbaatar Luvsan; Odontuya Gendaram; Bold Sharav; Ulambayar Lkhamsuren (Pharmacognosy Journal, 2023)
Background: It is important to determine and study in detail the action of the main chemical and secondary components contained in Paeonia anomala against diseases. In particular, it is of great practical significance to clarify the use of the plant from ancient medical books and scriptures and to determine which of the six main diseases were used in traditional medicine. Purpose: To compare the information of the ancient medical books written by Mongolian doctors and scientists, who wrote about Paeonia anomala L. and to clarify the taste, potency, strength, and quality. Research materials and methods: Research materials: Sumbe khamba Ishbaljir. “gso dpyad bdud rtsi 'i chu rgyun gyi cha lag gi nang tshan gyi sman so so'i mngon brjod dang ngos 'dzin shel dkar me long”. Wooden printing block with Tibetan script. Toin Jambaldorj. “gso byed bdud rtsi'i 'khrul med ngos 'dzin bzo rig me long du rnam par shar ba mdzes mtshar mig rgyan zhes bya ba bzhugs so”. Wooden printing block with Tibetan script. Research methods: Methods of textual analysis for ancient medical books and checklists were used in the study. Conclusion: The use of Paeonia anomala has been enriched with indications for use in traditional Mongolian medicine, Mongolian folk medicine, Russian folk medicine, and Chinese, Japanese, and Korean traditional medicine. In his book "ngos 'dzin shel dkar me long", the ancient medical scientist Sumbe khamba Ishbaljir wrote the name of the Paeonia anomala plant in Sanskrit as shogandha and in Tibetan as baspru. On the other hand, Toin Jambaldorj noted in his book "mtshar mig rgyan" wrote Paeonia anomala has a warm and equable quality, thus it can create potency. Therefore, it is appropriate to note that this plant in traditional Mongolian medicine is mainly used to treat back pain caused by cold conditions, chronic kidney disease, arthritis, uterine benign tumors, and bladder stones.

Elucidating the Uses of the Plant Veronica Incana from the Ancient Medical Books - Sansarkhuyag Enkhtur; Ariunjargal Tudev; Tungalag Dagdanbazar; Selenge Erdenechimeg; Odontuya Gendaram; Ulambayar Lkhamsuren; Bold Sharav (Pharmacognosy Journal, 2023)
Background: Medicinal plant research has been successfully carried out in the field of pharmacy and pharmacology in Mongolia, and dozens of research projects are still being carried out. However, there are still fewer studied medicinal plants of practical importance growing in Mongolia. Therefore, there is a need to register and study the ancient medicinal books written by ancient Mongolian doctors about the relatively little-studied Veronica Incana plant. In addition, taking into account that scientific research on Veronica Incana, which grows in Mongolia, has not been done in the field of medicine. Therefore, the topic was selected for research in this field. Purpose: Comparing the information of the ancient medical books written by Mongolian doctors and scientists, who wrote about Veronica Incana, to clarify the taste, power, and quality. Research materials and methods: 1. Research materials: Sumbe khamba Ishbaljir. gso dpyad bdud rtsi 'i chu rgyun gyi cha lag gi nang tshan gyi sman so so'i mngon brjod dang ngos 'dzin shel dkar me long. Wooden printing block with Tibetan script. Toin Jambaldorj. gso byed bdud rtsi'i 'khrul med ngos 'dzin bzo rig me long du rnam par shar ba mdzes mtshar mig rgyan zhes bya ba bzhugs so. Wooden printing block with Tibetan script. 2. Research methods: Methods of textual analysis for ancient books and checklist methods were used in the study. Conclusion: Gandhabhatra plant is used in traditional Mongolian medicinal practices from ancient times, the plant is called Veronica Incana in Latin. Also called Buural gandbadraa in Mongolian. On the other hand, the traditional medical doctors of China's Inner Mongolia and Tibet use Gandbadraa, the Latin name Gnaphalium affine D. Don. The appearance, shape, and color of the flowers of these two plants are different. According to the research of the source, the main instruction and usage of the plants are the same: to destroy benign tumors, to remove poison, to treat colds, and to stop cough.
Keywords: Ancient medicinal books.; Medicinal plants; Traditional Mongolian medicine; Veronica Incana

Emilia Sonchifolia-A Critical and Comprehensive Review of its Diverse Medicinal Potential and Future as Therapeutic - Sardar Hussain; Komal KP; Guruvayoorappan C (Pharmacognosy Journal, 2023)
Emilia sonchifolia (L.) DC, it belongs to the family Asteraceae or Compositae, is conventionally used in ethnomedicine, and acquires various medicinal properties. This plant has been regarded as one of the widespread traditional vegetable salads in Malaysia, Bangladesh, and India. In addendum to its avail as a vegetable, the plant has been documented with several medicinal benefits in the extravagance of night blindness, epilepsy, malaria, asthma, burns, breast abscesses, and inflammatory diseases. On scrutinization of their pharmacological properties, it has been revealed that they possess numerous notable biological properties such as antimicrobial, analgesic, anti-inflammatory, antioxidant, hepatoprotective, antianxiety, and anticataract, as well as anticonvulsant activities. Concrete evidence suggests the presence of potential phytochemicals in this plant with a wide range of unknown applications. In this current review, we discuss the phytochemicals present in the plant Emilia sonchifolia and emphasize the therapeutic and pharmacological activities reported so far concerning this plant.
Keywords: Anti-inflammatory; Anti-tumour; Emilia sonchifolia; Medicinal plant; Phytochemicals

Ethanolic Extract of Propolis from Tetragonula laeviceps: Selective Cytotoxicity for MCF-7 Breast Cancer cells - Rina Masadah; Savira Ekawardhani; Ramadani Eka Putra; Dzul Ikram; Muhammad Faruk (Pharmacognosy Journal, 2023)
Introduction: Many patients with breast cancer fail to respond to conventional chemotherapeutic agents; these agents are also associated with numerous adverse events and toxicities. These concerns have led to the ongoing search for natural ingredients with antitumor effects. As such, the aim of this study was to explore the anti-cancer properties of an ethanolic extract of propolis (EEP), a natural product derived from the stingless bee, from Tetragonula laeviceps. Methods: Bioactive components of EEP were identified by gas chromatography–mass spectrometry (GC–MS) and antioxidant capacity was tested by 2.2-diphenyl1-picrylhydrazyl (DPPH) analysis. Selective cytotoxic actions of EEP on both MCF-7 and Vero cells were then evaluated using the MTT assay. Polyphenols were identified as the major components of EEP from T. laeviceps. Results: our results indicated that EEP was selectively toxic for human MCF-7 breast cancer cells and had only limited impact on African Green Monkey kidney Vero cells. Conclusion: EEP from T. laeviceps has bioactive components that may selectively inhibit the proliferation of cancer cells. As such, EEP may be useful source material to be used for the development of novel anti-cancer agents.
Keywords: Breast cancer; MCF-7 Breast Cancer Cells; Proliferation; Propolis

Ethnobotanical Study of the Traditional Use and Maintenance of Medicinal Plant Species and Indigenous Knowledge by the Konso People, Southern Ethiopia - Tizazu Gebre; Bhaskar Rao Chinthapalli; D. S. Vijaya Chitra (Pharmacognosy Journal, 2023)
Background: For thousands of years, indigenous people have developed their own localized knowledge of plant use, management, and conservation. However, this rich traditional knowledge on plant utilization was not well documented, and most of the indigenous knowledge acquired by the local people was verbally passed from generation to generation. The people of Konso in South Ethiopia use and maintain traditional medicinal plant species, according to their specific ethnobotanical pharmacopeia. Methods: A total of 80 informants aged 25 to 87 years old were chosen (10 individuals of which 5 healers and 5 clients) from the 24 key informants were identified representing 6 from each kebele. The present study explores the unique indigenous knowledge that enables the community to identify medicinal plants, prepare medications, and then apply the cures used to treat a variety of human diseases as dangers to their long-term use. This study was carried out from September 2018 to July 2019. Results: Some medicinal plant species are more popular than others Lepidium sativum, Hagenia abyssinica and Allium sativum were cited by all the 80 informants for their medicinal value. Powdered form was directly used from 40.0% of the medicinal plant species, while 51.4% are chewed, extracted with water, and / or with butter (17.1% each) and 8.6% are extracted with local beer. Of the herbal remedies used in the study area, 68.6% were applied orally and 31.4% were applied externally. Roots are the parts mostly used (35.7%), followed by leaves (32.9%), combination of parts (18.6%), root bark (5.7%), fruit (2.9%), in that order, respectively. It was known that highest source of the medicinal plants was the forest (45.3%). Conclusions: Home gardens and agro forests together contribute 41.86% of the medicinal plants. This study was undertaken to bring awareness towards community-based in-situ and ex-situ conservation actions, attracting younger generations to grab the indigenous knowledge from the parents and ancestors and strengthening home gardens and other agro-forestry systems be used as repositories of medicinal plant species and alternative conservation sites to preserve the traditional medicinal plant species of the study area.
Keywords: Ethnobotany; Indigenous knowledge; Medicinal plant species.; Traditional healers

Evaluation of Antidiarrheal, Antispasmodic, and Antisecretory Activities of Extract and Fractions of Castanopsis costata Leaves in Animal Models - Maulana Yusuf Alkandahri; Mally Ghinan Sholih; Nitya Nurul Fadilah; Maya Arfania; Surya Amal; Dedy Frianto; Lina Aliyani Mardiana; Diany Astuti; Dadang Muhammad Hasyim (Pharmacognosy Journal, 2023)
Background: The use of traditional medicinal plants Castanopsis costata in the management of diarrhea is an ancient practice in North Sumatra, Indonesia, but its safety and efficacy have not been examined scientifically in animal models. Therefore, this study aims to determine the antidiarrheal effect of C. costata leaf extract and fractions in vivo. Methods: The antidiarrheal activity was evaluated against animal models of diarrhea induced by castor oil, charcoal meal, and entero-pooling test. The extract group received doses of 25, 50, 100, and 200 mg/kg, while the fraction groups was treated with WFCC, EAFCC, and nHFCC, in doses of 50 and 100 mg/kg, respectively. The negative control received 10 mL/kg of the dissolving vehicle, while the positive was treated with loperamide 3 mg/kg. Results: The results showed that EECC doses of 200 mg/kg, WFCC, and EAFCC at 50 and 100 mg/kg, respectively significantly delayed the onset of diarrhea, while WFCC and EAFCC at 100 mg/kg significantly reduced the amount of wet feces output and total feces output. In addition, only WFCC and EAFCC (at 50 and 100 mg/kg, respectively) significantly reduced the mean weight of wet feces and total feces. The percentage of maximum diarrhea inhibition was observed at the EAFCC dose of 100 mg/kg. Based on the charcoal meal test results, only EAFCC significantly inhibited the distance traveled by charcoal meal and reduced the peristaltic index at a dose of 100 mg/kg, while the percentage decrease in gastrointestinal motility was 46.87%. Similarly, in the entero-pooling test, WFCC and EAFCC significantly reduced the volume and weight of intestinal contents at a dose of 100 mg/kg, respectively. Conclusion: The results confirm that the extract and fractions of C. costata have antidiarrheal activity. Therefore, this study provides scientific support for the traditional use of C. costata in treating diarrhea.
Keywords: Animal models; Castanopsis costata; Diarrhea; North Sumatra.; Traditional medicine

Evaluation of Electronic Medical Record System in Outpatient Dr. Wahidin Sudirohusodo Makassar Central General Hospital in 2022 - Asriani Usman; Irwandy; Noer Bahry Noor; Alimin Maidin; Fridawaty Rivai; Indrabayu; Anwar Mallongi (Pharmacognosy Journal, 2023)
The Electronic Medical Record System in Indonesia is currently a concern of the government of the Republic of Indonesia by requiring every health facility including hospitals in Indonesia to implement an Electronic Medical Record System. To find out that this system is beneficial for health services in hospitals, an evaluation is needed. This study aims to evaluate the Electronic Medical Record System that has been running in the Outpatient of Dr. Wahidin Sudirohusodo Makassar Central General Hospital from a user perspective using the Human Organization Fit Model. The type of research used is quantitative using an analytical observational design with a cross sectional study approach. The sample in this study were users of electronic medical records in the Outpatient Department of Dr. Wahidin Sudirohusodo Makassar Central General Hospital, totaling 174 respondents. Hypothesis testing in this study using path analysis. The results of this study indicate that technology has a direct and significant effect on humans (<0,001) <0,05. Technology has a direct and significant effect on the organization (<0,001) <0,05. Humans have a direct and significant effect on the organization (<0,001) <0,05. Human has a direct and significant effect on benefits (<0,001) <0,05. Organization has a direct and significant effect on benefits (<0,001) <0,05. Technology has an indirect effect on benefits through people (ρ Value 0,007 < 0,05, path coefficient = 0,179). Technology has no indirect effect on benefits through organizations (ρ Value 0,051 > 0,05). This means that humans as intermediaries from technology to benefits have a significant effect while organizations as intermediaries from technology to benefits have an insignificant effect.
Keywords: Electronic Medical Record System; Hot-Fit; Outpatient.; System Evaluation

Evaluation of In vivo Analgesic and Anti-inflammatory Activity of Oroxyulum indicum, Baicalein, Chrysin with Phytochemical Analysis and Molecular Docking Study - Bhairav Kumar Pathak; Kamlesh M. Palandurkar; Meenakshi Singh; Anshuman Trigunayat; Amit Singh; Reena Giri; Kiran Rajendra Giri (Pharmacognosy Journal, 2023)
Background: Oroxyulum indicum (OIE) is a native medicinal plant that has been widely employed in Ayurvedic medicine for thousands of years. Though studies have been published citing the analgesic and anti-inflammatory activity of Oroxyulum indicum and chrysin and Baicalein, there has been no comparative study comparing their activittes and confirming them with molecular docking results. Molecular docking study of two phytochemicals Chrysin (PubChem CID 5281607) and Baicalein (PubChem CID 5281605) into the active sites of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Evaluation and validation of Anti-inflammatory and Analgesic effects of a methanolic extract of the stem bark of Oroxylum indicum and its two constituents Chrysin and Baicalein in Charles foster rats with analysis of the phytoconstituent of Oroxyulum indicum through HRMS analysis. Methodology: UHPLC-HRMS/MS analyses were performed on a Dionex Ultimate 3000 RS Series UHPLC system combined with a Q Exactive Plus High-Resolution Accurate Mass Spectrometry System. Hot plate and Tal flick model are used for screening of analgesic activity. TNF-alpha and IL-6 inflammatory markers were examined. Carrageenan model is used for antiinflammatory analysis. Result: Interesting results has been obtained in the docking studies of Chrysin and Baicalein with COX-1 (PDB ID: 1EQG). The hydrogen bond interaction established between the Chrysin and Baicalein with the important amino acid, includes Arg 120, Tyr 355, Ser 530, Met 522 (Figure 1). The binding free energy of the Chrysin and Baicalein with target COX-1 was found to be -7.88 and -7.26 Kcal/mol. Conclusion: There is marked reduction in the TNF Alpha expression in the OIE group which is followed by Baicalein and Chrysine. The Baicalein group shows the most marked cumulative increase in reaction time for tail flick among all the groups of the intervention group followed by Chrysine and OIE.
Keywords: Baicalein; Chrysine; Molecular docking; Oroxylum indicum; TNF alpha.

Evaluation of Neuroprotective Efficacy of Indian Shankhpushpi Varieties in Alzheimer’s disease – North Vs South - Sharada L. Deore; Anjali A. Kide; Bhushan A. Baviskar; Somshekhar S. Khadabadi; Bhavana A Shende (Pharmacognosy Journal, 2023)
Background: Shankhpushpi is a well-known Ayurvedic memory enhancing medicine associated with controversy. Among the various varieties, Convolvulus pleuricaulis and Clitorea ternatia are widely used in commercial memory enhancing formulations under the name of Shankhpushpi. Convolvulus pleuricaulis is recognized as true shankhpushpi in North side of India, however in southern part of India, Clitorea ternatia is accepted as Shankhpushpi. Objectives: The present study aims to compare neuroprotective efficacy of C. pleuricaulis and C. ternatea by in vitro, in vivo methods and establish scientifically validated data to choose appropriate shankhpushpi variety for commercial use. Materials and Methods: Both herbs were extracted in the Soxhlet apparatus with 70% ethanol for 5 - 6 hours at 60 - 65̊ C. The presence of neuroprotective principles - taraxerol and scopoletin in extracts was confirmed by the Thin Layer Chromatography. The preliminary screening for neuroprotective efficacy of extracts was done by in vitro free radical scavenging, Acetyl cholinesterase enzyme inhibition and LOX enzyme level estimation. Invivo study of extracts included behavioral assessment of adult rats by Y maze, Morris water maze using scopolamine induced Alzheimer’s disease like model. Result: Extracts of both C. pleuricaulis and C. ternatea significantly scavenged free radicals, inhibited acetyl cholinesterase and LOX enzyme in vitro. But in in-vivo study, significant retention of spatial and working memory was observed in rats administered with C. pleuricaulis as compared to C. ternatea. Conclusion: C. pleuricaulis more significantly shields against memory loss and dementia by reducing oxidative stress, inflammation, and memory impairment. Hence should be used in commericial neuroprotective formulation as chief source of Shankhpushpi instead of C. ternatea.
Keywords: Alzheimer’s disease; Dementia; Scopolamine; Scopoletin.; taraxerol

Evaluation of the Economic Impact of Implementing National Health Insurance (JKN) on Hospitals at RSUD Tenriawaru Kab. bone - Karmilasari; Sukri Palutturi; Darmawansyah; Muh. Syafar; Indar; Citrakesumasari; Anwar Mallongi (Pharmacognosy Journal, 2023)
The National Health Insurance Program (JKN) through the Social Security Administering Body has been running since January 1 2014, the Tenriawaru Regional General Hospital in Bone Regency is one of the hospitals under the auspices of the local government that works closely with BPJS Kesehatan. The Bone Regency Government has registered 237,381 people into the National Health Insurance (JKN) program. In general, this study aims to analyze the economic impact of the implementation of the National Health Insurance (JKN) on hospital income, hospital financing and financial compensation for hospital employees at Tenriawaru Hospital, Kab, Bone. This study uses a qualitative descriptive research method that aims to obtain an in-depth evaluation study on evaluating the economic impact of the implementation of the National Health Insurance (JKN) program on hospitals. The informants in this study totaled 5 people consisting of the Head of Finance Subdivision, the JKN case mix officer, the Head of Service Subdivision, the Head of HR Subdivision and the Head of Public Relations Subsection. The results of the study show that the implementation of the National Health Insurance (JKN) program has an impact on hospital income. The implementation of JKN provides great benefits for the hospital. the implementation of the National Health Insurance (JKN) program also has an impact on hospital financing, JKN covers hospital operational costs, JKN claims have their respective portions for hospital human resources, drug resources and BHP in hospitals. The implementation of the National Health Insurance (JKN) program has no impact on the financial compensation of employees in hospitals, salaries, bonuses and semi-annual incentives that employees get have no effect on the implementation of JKN in service hospitals that get a portion each for the operational needs of the hospital not for employee financial compensation.
Keywords: Compensation; Financial; Financing; Income; JKN

An Evaluation of the Effectiveness of Pharmacognosy Research and a Pharmaceutical Resource Management in Hospitals - Suresh Chandra Akula; Pritpal Singh; Prikshat Kumar Angra; Gagandeep Singh Cheema; Anup Sharma; Mohd Farhan (Pharmacognosy Journal, 2023)
Objective: This research paper aims to evaluate the relative efficacy of pharmacognosy research on the top 40 tertiary hospitals and its pharmacy in India according to Newsweek's 2021 ranking. Purpose: India's pharmaceutical market is now estimated to be worth $50 billion. India is a significant pharmaceutical exporter, reaching more than 200 nations with its exports. This study will help to identify whether the pharma industry is working efficiently in selected Indian hospitals or not. Thus, to create a bench marking for the hospital pharmacies in India. Design/methodology/approach: Data for the study came from public and private hospitals' official websites, financial records, and government notifications. Cross- sectional data analyses were done using a Slack-Based model and data envelopment analysis (DEA). STATA was the software used to scrutinize the results effectively. Results: Thirteen hospital pharmacies out of a total of 40 are judged to be relatively technically efficient overall, according to the research. The findings of the slack analysis demonstrate that government hospitals and pharmacies are better able to handle slack and effectively manage input and output. Conclusion: The researchers also set upped a benchmarking hospital both for private and government healthcare settings for pharmacognosy research. Thus, it can be utilized for the betterment of the pharmacies.
Keywords: Data envelopment analysis (DEA); Efficiency; Hospitals; India; Pharmacognosy research; Pharmacy; SBM model

Experiences and Expectations of Post-Partum Mothers Toward Psychosocial Nursing Services for Mothers Who Have Experienced Postpartum Blues in City of Balikpapan - Ns. Grace C Sipasulta; Indah Nur Imamah; Genevieva E Tanihatu (Pharmacognosy Journal, 2023)
After giving birth, the mother feels happy to finally be able to meet her beloved baby. However, some mothers actually do not feel happy but appear feelings of sadness, anxiety, and depression after giving birth. As many as 30-75 present of women can experience baby blues after giving birth and one in seven women can experience postpartum depression. Health Education needs to be improved and Nutrition for pregnant women needs attention Micronutrients prevent postpartum depression so that babies are born healthy. Based on the above problems that are still untouched by promotion and prevention health for women overcome the level of emotional anxiety from pregnancy to post-delivery which can be experienced by mothers from mild, moderate to severe. Nurses play a key role related to providing physical and psychological care to support the improvement of the mother's condition towards more positive. Mothers are at risk for natural emotional problems requiring someone to be able to give him an understanding of the process he is going through and help him to remain in a more stable condition. This study involved seven participants with diverse characteristics and provided an overview and the high need of postpartum mothers to feel comfortable so that their coping efforts could be achieved so that they did not experience emotional problems after giving birth.
Keywords: Baby Blues; Depression; Postpartum; Psychosocial Services.

Experimental Study of Development Food Safety Booklet using Local Language - Ridwan Mochtar Thaha (Pharmacognosy Journal, 2023)
To prevent food poisoning in Indonesia, it is necessary to have health promotion efforts in the field of food safety using appropriate media. Therefore, this study aims to develop food safety information media, namely local language booklets. For this reason, this study examines the validity and feasibility of local language food safety booklet. The research was conducted in Gorontalo Province from August to October 2022. The development of booklet is done by validating and trial. Validation was carried out using the DELPHI technique with experts in a qualitative descriptive research type. After that, a trial of the booklet was carried out with 20 Village Food Safety Cadres by distributing trial questionnaires and short interviews. Data analysis in this study used content analysis techniques. The results of the validity and feasibility of the local language food safety booklet show that the booklet is very feasible to use. Furthermore, it is hoped that further research can examine the effectiveness of using booklets to convey food safety messages to the wider community.
Keywords: Booklet; Cadre; Food Safety

Expressions of Matrix Metalloproteinase-3 and Tissue Inhibitor Metalloproteinase-1 in Corneal Tissue Post Alkali Burn Treated with Topical Medroxyprogesterone Acetate and Doxycycline - Daya Banyu Bening; Reni Prastyani; Nurwasis; Evelyn Komaratih; Ismi Zuhria; Hari Basuki Notobroto; Dyah Fauziah; Chrismawan Ardianto (Pharmacognosy Journal, 2023)
Purpose: This study aims to investigate the effects of topical Medroxyprogesterone acetate (MPA) and Doxycycline in inhibiting the expression of MMP-3 and TIMP-1 in ocular alkali burn models in animals. Methods: A total of 18 New Zealand Rabbits were divided into 3 groups based on their post-alkali-burn treatment: PBS (G1/ control group), topical Doxycycline 1mg/ml (G2), and topical MPA 1% (G3). Alkali burn models were made by exposing 1N NaOH solution to the central cornea for 30 seconds. MMP-3 and TIMP-1 expression were evaluated using immunohistochemistry after 14 days of treatment. Results: Statistically significant differences in the mean MMP-3 expression were found between the three groups (p=0.010). There was a significant difference in MMP-3 expression between the control group with MPA (p=0.017) and Doxycycline (p=0.028) but was not found between the MPA and Doxycycline groups (p=1,000). The mean differences in TIMP-1 expression between the three treatment groups were statistically significant (p=0.005), with a significant difference between the control group with Doxycycline (p=0.022) and MPA (p=0.007). There was no significant difference in TIMP-1 expression between the Doxycycline and MPA groups (P=1,000). Conclusion: This study indicated that topical administration of Doxycycline or MPA in ocular alkali burn reduces the expression of MMP-3 and TIMP-1.
Keywords: Corneal alkali burn; Doxycycline.; Medroxyprogesterone acetate

Extract of Torbangun (Coleus amboinicus Lour) Leaves Inhibits the Expression of PEPCK and G6Pase in Streptozotocin-Induced Diabetic Rats - Meilla Dwi Andrestian; Rizal Damanik; Faisal Anwar; Nancy Dewi Yuliana; Anwar Mallongi (Pharmacognosy Journal, 2023)
Objective: This study aimed to analyze the anti-hyperglycemic activity of Torbangun leaf extract (TLE) in hyperglycemic rats by observing its effect on blood glucose levels and PEPCK and G6Pase gene expression. Method: This post-test-controlled group design study used a completely randomized design (CRD). Seven rats were separated as a normal group (N) and other rats were injected with streptozotocin (STZ). Hyperglycemia was characterized by fasting blood glucose (FBG) >126 mg/dl. Hyperglycemic rats were divided into three groups: H-IM (control drug metformin hydrochloride dose 62.5 mg/kg BW), H-IT (TLE dose 620mg/kg BB), and NG (negative control, hyperglycemic rats). Rats were given intervention for 14 days. Result: The results showed a significant difference between initial and final FBG of the HI-IM group (p = 0.002, α = 0.01) and the H-IT group (p = 0.005, α = 0.01) and effect on the key gene expression of gluconeogenesis in the liver. The H-IT group showed a decrease in PEPCK gene expression 0.80 times and decreased G6Pase gene expression by 0.65 times compared to NG group. Conclusion: The findings suggested the administration of TLE in STZ-induced hyperglycemic rats was able to reduce blood sugar levels and was involved in inhibiting the expression of PEPCK and G6Pase expression genes in the liver.
Keywords: G6Pase; hyperglycemia; PEPCK; Rats; Torbangun

Factors Affecting the Long-Term Protection Against Hepatitis B Immunization in Infancy: A Meta-Analysis - Aldila Pratiwi; Bagus Setyoboedi; Citrawati D. K. Wungu (Pharmacognosy Journal, 2023)
Introduction: Hepatitis B virus (HBV) infection is a major global health issues and one of the most dangerous viral infections with a high mortality rate. Newborns and infant vaccination against chronic HBV infection are crucial for preventing mother-to-child transmission (MTCT). This study aimed to conduct a meta-analysis to investigate the factors affecting long-term protection against Hepatitis B Immunization in infancy. Material and Methods: Our literature searches are from PubMed, Science Direct, Web of Science, and ProQuest publications between January 2000 and December 2021. The included literature assessed the risk of bias using the Newcastle Ottawa Quality Assessment Scale. We identify Hepatitis B surface antibodies (anti-HBs) ≥ 10 mIU/mL as being protective against HBV infection. The results are combined with a random effect or fixed effect model. Results: Eighteen eligible observational studies with a total of 16,642 participants were included. Analysis of factors affecting long-term protection status by assessing anti-HBs titers showed significant results on several factors, including gestational age for anti-HBs titers (OR 2.5; 95% CI 1.62-3.85; p<0.0001), weight for age to anti-HBs titers (OR 1.36; 95% CI 1.06-1.75; p=0.02), length for age to anti-HBs titers (OR 0.01; 95% CI 0.01-0.02; p<0.00001), and immunization status based on the number of vaccine doses (4 doses vs 3 doses) to anti-HBs titers (p<0.00001). Conclusions: Anti-HBs titers of hepatitis B immunization were significantly affected by gestational age, weight for age, length for age, and vaccine doses. Parents of newborns must be informed about basic immunization and provide adequate nutritional intake to the mother and babies to prevent HBV infection.
Keywords: Hepatitis B antibodies; Hepatitis B vaccines; Immunity; Immunization; Infant.

Factors Affecting the Occurrence of Diarrhea in Infants Aged 7-12 Months in Puskesmas Alak Kupang City - Deviarbi Sakke Tira; Sonyalin Buifena; Sigit Purnawan; Maria Magdalena Dwi Wahyuni; Marni Marni; Anwar Mallongi (Pharmacognosy Journal, 2023)
Diarrhea is a disease characterized by changes in the consistency of stools to liquid accompanied by an increase in the frequency of defecation more than usual > 3 times / day accompanied by changes, with or without blood and or mucus. Factors that cause diarrheal disease are infections, infections can be caused by bacteria, parasites and viruses. Busy parents often pay less attention to health and the impact of an unhealthy environment. The three children have digestive problems, which are still considered a habit and can be cured without having to go to a health facility. The purpose of this study was to identify and analyze the factors that influence the incidence of diarrheal disease in infants aged 7-12 months at the Alak Health Center, Kupang City. The type of research used is an analytical survey with a case control design. The population in this study was divided into case populations, namely all infants aged 7-12 months totaling 137 infants and controls were infants aged 7-12 months who did not experience diarrhea totaling 8,868 infants. Determination of the size of the control sample using a ratio of 1:1 with the case sample so that the number of control samples is the same as the case sample, namely 37 infants and the total sample is 74 infants. The sampling technique is simple random sampling. Analysis of the data used is the chisquare test. The results showed that there was a significant relationship with a history of premature birth (p-value = 0.015 with an odds ratio = 3.434), low birth weight (p-value = 0.015 with an odds ratio = 4.563), exclusive breastfeeding (p-value = 0.015 with an odds value ratio = 3.434), immunization status (p-value = 0.004 with odds ratio = 4.265) and history of allergies (p-value = 0.004 with odds ratio = 4.083) with the incidence of diarrheal disease at the Alak Health Center, Kupang City. Suggestions that health center health workers need to be able to increase prevention efforts from an early age by approaching the community and providing concrete education related to the incidence of diarrhea.
Keywords: BBLR; Diarrhea; Exclusive breastfeeding; Immunization Status; Premature

Generation of Multinucleated Giant Cells due to Leishmania (V.) braziliensis Infection - Jesús Rojas-Jaimes; Luis Castañeda Pelaez (Pharmacognosy Journal, 2023)
Background: Multinucleated giant cells were first described by Langhans(MGCs), these MGCs have a role in innate immunity that includes extracellular matrix remodeling associated with granuloma formation; another function of them is to participate in the removal of cellular debris in apoptosis during certain infections. Objective: Generate multinucleated giant cells due to Leishmania (V.) braziliensis infection. Methods: For infection macrophage/parasite ratio of 1 to 10 using the RAW 264.7-line (5 x105/mL) and Leishmania (V.) braziliensis metacyclic promastigotes (50 x105/mL) was a strain maintained in culture Schneider liquid medium, supplemented with 20% fetal bovine serum and 10 000 U/10 mg/mL penicillin streptomycin at pH 7 and 25 °C in the laboratory. Incubation was continued for 4 days, and microscopic observation at 1000X was performed at 24 hours and 96 hours, respectively. Results: In our study an MGC conversion was observed at 96 hours (50% ± 28.2). Conclusions: In vitro MGC model could be used to study the physiopathology of MGC generation by Leishmania (V.) braziliensis infection.
Keywords: Immunity; Infection; Leishmania (V.) braziliensis; Macrophage; Multinucleated Giant Cells

Habitat Characteristics of Aedes Sp Larval Containers and Density of Container Index (CI) In the Area Endemic and Non-Endemic to DHF In Makassar City - Muh Ichsan; Hasanuddin Ishak; Erniwati Ibrahim; Hasnawati Amqam; Isra Wahid; Syahribulan; Rusdiyah Sudirman; Anwar Mallongi (Pharmacognosy Journal, 2023)
Aedes sp has breeding habitats in water reservoirs or containers with relatively clear water, both inside and outside the house and in public places. The existence of containers as a breeding habitat for Aedes sp mosquitoes can increase the population density of Aedes sp mosquitoes, triggering high transmission of the dengu virus in the city of Makassar. The purpose of the study is to determine the characterisitic relationship of containers with the index of aedes sp larvae in dengue endemism areas in Makassar city in 2022. This study is an analytical observational study. Quantitative analysis with a cross-sectional approach. The sample sampling technique is a technique with simple purposive sampling. The sample was 600 houses in endemic and non-endemic areas in makassar city. The results of this study are container types with CI density in endemic areas, namely bucket 242 (CI = 9.5), while in non-endemic areas namely bucket 298 (CI = 5.4), container materials with CI density in endemic areas namely plastic 422 (CI = 10.9), while in non-endemic areas namely plastic 482 (CI = 5.6), and container locations with CI materials and densities namely in indoor locations with plastic material 398 (CI = 8.0), while in non-endemic areas with CI materials and densities, namely in indoor locations with plastic material 455 (CI = 5.5). The results of the chi-square test in the endemic area obtained a value of p = 0.478 which means that there is no relationship between the container type and the presence of aedes sp larvae in Tamamaung, Chi-square test results in non-endemic areas obtained a value of p = 0.217 which means that there is no relationship between the container type and the presence of Aedes sp larvae in the new Malimangan Village. The results of the chi-square test in the endemic area obtained a value of p = 0.000 which means that there is a relationship between the location and the presence of aedes sp larvae in tamamaung, and Meanwhile, the results of the Chi-Square test in non-endemic areas obtained a value of p = 0.631 which means that there is no relationship between the location and the presence of Aedes sp larvae in the new malimongan. There is no relationship between CI density and endemic and nonendemic regions. All six regions fall into the moderate category. His advice for the Makassar City Health Office, especially in Tamamaung, Pisang Utara, Pampang, Malimongan Baru, Kalukuang and Rappojawa villages, is that it is necessary to conduct counseling / information about the importance of environmental cleanliness and mosquito breeding sites to residents evenly and distribute abate, so that the community can be vigilant and avoid the transmission of dengue fever
Keywords: Characteristics of Container Habitats; Endemic and Non-Endemic.; Larval Density

Hand Hygiene Awareness and Knowledge Among Healthcare Personnel in The Dawadmi Region of Saudi Arabia: A Cross- Sectional Survey Study - Prasanna Mohana Bhaskaran; Mohammed Nawar Alotaibi; Inbaraj Susai Domnic; Bhaskaran Ashokan; Sudharshan Reddy Dachani; Semmal Syed Meerasa; Osama Mohammed Alruways (Pharmacognosy Journal, 2023)
Introduction: The outcome of patient care in healthcare settings is influenced predominantly by healthcare-associated infections (HAIs). Hand hygiene is the single most essential component in avoiding HAIs. Objectives: The aim of the study is to evaluate the awareness and knowledge about hand hygiene among the healthcare personnel in the Dawadmi Region of Saudi Arabia. Materials and Methods: This cross-sectional survey study was carried out between May till September 2021. The tool (Questionnaire) was adopted from World Health Organization's Hand Hygiene Knowledge and awareness Questionnaire (revised in 2009), validated according to CROSS (Checklist for Reporting of Survey Studies) check list. Results: A total of 300 participants were randomly enrolled, 60 were nursing assistants, 140 nurses, and 100 residents, were enrolled in this study. With respect to years of experience in hand washing, 123 participants had 1–5 years (41%), 6–10 years (80%), 11–20 years (77%), and greater than 30 years of participation (6.67%). Concerning the education of hand washing and hygiene, training programs were attended by 230 participants (76.67%), and 70 participants (23.33%) did not attend the latest training program conducted by each institution. Most of them (83.33%) were using alcohol for hand hygiene. Conclusions: This study has clearly shown the requirement of an in-depth appraisal of important issues of compliance and patient safety. Educational interventions to recognize the hand hygiene opportunities, improved availability of hand hygiene facilities and multifaceted approach to tackle various barriers (poor attitude, workload, etc) of adherence are needed to be accorded priority.
Keywords: awareness; Cross infection; Hand hygiene; Healthcare workers; Knowledge.

Health Behavior and Social Support in Postpartum Mothers Treatment in Ba'ukup Tradition in Maluku - Andi Asrina; Rizal Payapo; Fairus Concerned Idris; Sukri Palutturi; Anwar Mallongi (Pharmacognosy Journal, 2023)
The maternal mortality rate is still high in Indonesia and one of the priorities of government agendas to decrease, one of them is giving good treatment to Postpartum Mothers. This is very important because in the Postpartum periode, there is a risk to get bleeding and infection that has an impact on maternal death. Many Postpartum Mothers' treatment is based on local wisdom, but not all give benefits to Postpartum Mothers. The aim of this study was to analyze Ba’ukup Tradition as a Postpartum Mothers Treatment Based on Local Wisdom in Negeri Luhu, Huamual, Maluku. This study used Qualitative method with ethnographic approach. The informants were 8 people which consisted of 1 supporting informant (Traditional Midwife/ Mama Biang), 1 Midwife as a key informant and 6 ordinary informants were selected purposively with the criteria of Mothers who are carrying out Ba'ukup. Thematic data analysis and data validity used triangulation technique. The results showed that Ba’ukup tradition is an ancestral heritage felt psychically and beneficially by Postpartum Mothers such as feeling more relaxed, sleeping better, sweating and helping to increase breast milk production. Meanwhile, Ba’ukup tradition is a local wisdom in Postpartum Mothers treatment so that in its implementation, it requires the assistance of health workers because during the Postpartum period, there is a risk to get bleeding and other complications. It is suggested that there are persuasive approach and communication between health workers and the society in order to build a harmonious trust in local traditions and health services.
Keywords: Ba'ukup; Health.; Postpartum; Tradition

Health Literacy Brisk Walking Exercise on Clinical Outcomes of Blood Sugar in Patients with Type 2 Diabetes Mellitus in Indonesia - Zainuddin; Andi Zulkifli Abdullah; Nurhaedar Jafar; Suriah; Nursalam; Darmawansyah; Syahrul Syahrul; Wahiduddin; Widiantoro; Anwar Mallongi (Pharmacognosy Journal, 2023)
Background: Regular Physical activity (PA) is recommended for patients with type 2 diabetes because it has a beneficial effect on metabolic risk factors for the development of diabetic complications. This study was designed to determine the effect of health literacy (HL) brisk walking on clinical outcomes (HbA1c) and PA in patients with DMT2. Method: In using the Quasi Experimental method using the Two group pre-posttest design approach. Research subjects were allocated for the provision of Health literacy Brisk walking (n-30, 30 completed) and the usual care group (n = 30,30 completed). Health literacy brisk walking participants received weekly scheduled information via WhatsApp group with monitoring to walk 3-5 times for 150 minutes/week, while the control group only received regular care from the Chronic Disease Management (Prolanis) program. Results: Sixty T2DM patients (thirty in the intervention group and thirty in the control group) were examined. The results showed that Health literacy Brisk walking with Whatsapp Group Monitoring in the intervention group was significantly higher than usual care from the Chronic Disease Management program (Prolanis) in the control group towards changes in HbA1c and PA after 3 months (p<0.05). Conclusion: Health literacy brisk walking related to diabetes can be a modifiable factor related to HbA1c for DMT2 patients. The findings indicate the need for health care providers to be able to consider brisk walking PA as a solution in controlling blood sugar (HbA1c).
Keywords: Blood Sugar (HbA1c).; DMT2; Health Literacy Brisk walking; Physical Activity

Hepatitis E Inhibited by Rosmarinic Acid Extract from Clove Plant (Syzygium Aromaricum) through Computational Analysis - Sunadi; Saddam Al Aziz; Fadhilah Fitri; Devni Prima Sari; Muhammad Raffi Ghifari; Rismi Verawati; Nita Yessirita; Oski Illiandri; Riso Sari Mandeli; Devi Purnamasari; Putri Azhari; Rahadian Zainul; et al. (Pharmacognosy Journal, 2023)
This study aims to evaluate the potential of Rosmarinic Acid as an inhibitor against Hepatitis E by interacting with the active site of the Tyrosine FYN protein. Computational approaches were employed to predict the molecular interactions between Rosmarinic Acid and Tyrosine FYN. The research methodology involved the use of software such as Pymol, Pyrex, Protein Plus, and the Lepinski Rule. Docking analysis was conducted using Pymol to obtain information about the binding energy between Rosmarinic Acid and Tyrosine FYN. The results of the analysis showed that Rosmarinic Acid exhibited a Binding Affinity of -8.3, -8, and -7.9, indicating a strong affinity towards the target protein. Additionally, Root Mean Square Deviation (RMSD) values of 0, 15.905, and 17.014 were used to assess the stability of the formed protein-ligand complex. Analysis using Protein Plus revealed interactions between Rosmarinic Acid and Tyrosine FYN. Furthermore, analysis using the Lepinski Rule to examine the physicochemical properties of Rosmarinic Acid indicated that the molecule had a mass of 360, 5 hydrogen bond donors, 8 hydrogen bond acceptors, a log P value of 1.76, and a molar reactivity of 89.8. These findings highlight the potential of Rosmarinic Acid as an inhibitor of Hepatitis E through its interaction with the Tyrosine FYN protein, providing a basis for the development of potential new therapies in the treatment of this disease.
Keywords: Hepatitis E; Molecular Docking.; Rosmarinic acid; Syzygium aromaricum; Tyrosine FYN

HPLC Isolation of Rutin, Hesperidin and Quercetin from Ruta Chalepensis Extract Growing in Iraq - Ali Mohammed Zaki Al-Jaberi; Sabaa Ali Mohammed Al-Fadal; Thukaa Zuhair Abdul-Jalil; Haider Al-Wafi (Pharmacognosy Journal, 2023)
Background: Ethnopharmacological relevance: Ruta chalepensis L For years, various portions of this plant have been utilized in traditional medicine to treat a number of illnesses, including nervous disorders, rheumatism, menstrual issues, fever, convulsions, mental disorders, dropsy, and neuralgia. Objection: In this study, we aim to characterize three flavonoids extracted from Ruta chalepensis L. Material and Methods: In this study, various concentrations (80% and 100%) of methanol and ethanol in water were used as solvents in the extraction of flavonoids (rutin, hesperidin, and quercetin) from Ruta chalepensis L. by using conventional extraction methods (reflex and maceration). Result: HPLC results confirm Using maceration with 80% methanol was recommended for extracting flavonoids from Ruta chalepensis L. to obtain the highest yields, whereas reflex with 80% ethanol showed a slight increase in quercetin amount. The phytochemical screening of Ruta chalepensis L. exhibited the presence of flavonoids. Conclusion: The highest amount of the three flavonoids was found in maceration with 80% methanol.
Keywords: Ethanol; Extraction.; Maceration; Methanol; Reflex

HPTLC Comparision Studies with Marker Compound and Isolation of Rutin Anti Inflammatory Agent from Ethanolic Leaf Extract of Rivea Hypocrateriformis (Desr.) Choisy - Shanthini Nachiar G; Thirumal M (Pharmacognosy Journal, 2023)
Introduction: To extract the leafy parts with ethanol and perform the HPTLC studies with marker compounds Quercetin, Rutin, and gallic acid then a compound was isolated and susceptible to spectroscopic analysis Background: Rivea hypocrateriformis (Desr.) Choisy holds Convolvulaceae family the leafy parts underwent a Soxhlet extraction with ethanol which was then subjected to a phytochemical investigation that shows the presence of alkaloids, phenols, flavonoids, terpenoids, tannins, glycosides saponins, sterols, and carbohydrates. Material and Methods: Firstly, the ethanolic leaf extract is treated with petroleum ether to remove excess lipids, sterols, and impurities. TLC was performed by toluene: ethyl acetate: formic acid and the same solvent system was used for column chromatography. The bands getting separated into the column were collected separately in a test tube. Once the solvent toluene ends, the next solvent system with 18 ml of toluene and 2 ml of ethyl acetate, is introduced into the column, and continues the separation procedure. Collect separated bands and perform TLC of each separate band by using the same solvent system in a concentration of 5:4:1. A single component on a TLC plate with a solvent system of 12 ml toluene and 8 ml ethyl acetate was obtained whose characterization was done by IR, NMR, and mass. Results: The RF value of Rutin is 0.17 which correlates with the Retention factor of ethanolic leaf extracts of RH by HPTLC comparative studies and a compound was isolated by column chromatography. Conclusion: These studies have concluded that a compound is separated by means of HPTLC and spectroscopic interpretation and identified as Rutin. <quillbot-extension-portal></quillbot-extension-portal>
Keywords: Column chromatography; HPTLC studies; Marker compound; Rivea hypocrateriformis ethanolic leaf extract; TLC plates.

Hydrocephalus Mice Model: Choroid Plexus Aquaporin-1 Dynamics Following Cerebrospinal Fluid Drainage - Yusuf Baktir; Muhammad Arifin Parenrengi; Wihasto Suryaningtyas; Dyah Fauziah; I Ketut Sudiana; Budi Utomo (Pharmacognosy Journal, 2023)
Background: Aquaporins (AQPs) are a family of membrane proteins that act as channels for water, facilitating its movement across the plasma membrane of cells. Aquaporin1 (AQP1), located in the choroid plexus, is thought to be involved in the process of cerebrospinal fluid (CSF) production. Objective: The objective of this study is to examine the impact of hydrocephalus and cerebrospinal fluid (CSF) drainage on the expression of AQP1 in a mice model of hydrocephalus. Material and Methods: Laboratory experimental study with six groups. Five test groups, one control group, and a rat model of hydrocephalus caused by kaolin were used in the experiment. Results: Hydrocephalus in mice model induced by kaolin, and CSF drainage was performed on the 7th and 14th days group. Immunohistochemical analysis was conducted to examine the presence of AQP1 in the choroid plexus using microscopes. The findings revealed a noticeable decrease in AQP1 expression levels in the choroid plexus, which exhibited a semi-quantitative decline in correlation with the duration of hydrocephalus (p = 0.01). This decrease was observed when comparing the normal group with the hydrocephalus groups on the 7th, 14th, and 21st days following induction. However, after cerebrospinal fluid (CSF) drainage, there was a significant increase in AQP1 expression (p < 0.05). Conclusions: This study shows the significant role of AQP1 in CSF production by comparing of AQP1 expression in the choroid plexus of hydrocephalus mice model, with and without CSF drainage. AQP1 expression experiences downregulation in hydrocephalus mice model and upregulation after CSF drainage.
Keywords: AQP1; Aquaporin 1; Choroid plexus; Hydrocephalus.

Identification of Family Adaptive Behavior in The Dayak Tribe in An Effort to Prevent Stroke - Parellangi; Muhammad Syafar; Sukri Palutturi; Agus Bintara Birawida; Anwar Mallongi; Oedojo Soedirham; Pawennari Hijjang (Pharmacognosy Journal, 2023)
The Dayak tribe inhabits a large part of West Kutai Regency, East Kalimantan Province. Hypertension is a major health problem in the West Kutai region. The family plays a role as a facilitator in reducing hypertension and preventing the risk of Stroke in family members. The purpose of this study was to identify the adaptive behavior of Dayak families in an effort to prevent Stroke. This research method is qualitative research with in-depth interviews and FGD (focus group discussion). FGDs were conducted on 7 participants with tingkat 1 and tingkat 2 hypertension patients and 3 people from traditional leaders/ culturalists. This study obtained four main themes, namely 1) Understanding health problems, 2) Utilization of medicinal plants, 3) Disease control, 4) Driving factors in reducing Stroke risk. Suggestions for health workers to be able to improve educational methods and digital health promotion not only using print media.
Keywords: Family Adaptive Behavior.; FGD; Stroke; The Dayak Tribe

Immunization During the Covid-19 Pandemic - Yuniarti; Rusmilawaty; Tri Tunggal; Norlaila Sofia; Nur Rohmah Prihatanti; Ahmad Rizani; Anwar Mallongi (Pharmacognosy Journal, 2023)
Objectives: This Study Aims to Analyze the Determinant Factors Associated with Basic Immunization Services for Infants Aged 1-12 Months in Banjarbaru City During the Covid 19 Pandemic. Methods: The Research Design Is an Analytic Research with A Cross Sectional Approach. The Independent Variables Are Mother's Knowledge, Mother's Anxiety, And Accessibility to Health Facilities, And the Dependent Variable Is Basic Immunization Services. The Research Sample Consisted Of 100 Respondents Who Had Babies Aged 1-12 Months in The Cempaka Health Center, Sungai Ulin Health Center, Sungai Besar Health Center and South Banjarbaru Health Center. The Sampling Technique Was Non-Probability Sampling, Namely Purposive Sampling. The Instruments Used Were A Knowledge Questionnaire, An Anxiety Questionnaire (Dass) And an Accessibility Questionnaire in The Form of a Google Form. Analysis Using the Chi Square Statistical Test. Results: The Results Showed That 58 People (58.0%) Had Basic Immunization Services According to Age, 44 People (44.0%) Had Good Knowledge And 55 People (55.0%) Had Accessibility to Health Facilities. Variables Related to Basic Immunization Services in Banjarbaru City Were Knowledge (P=0.03) And Anxiety (P=0.01). Another Variable in This Study, That Is Accessibility to Health Services, Was Not Related to Basic Immunization Services in Banjarbaru City (P=0.80). Conclusions: It Is Recommended That Basic Immunization Information and Services Be More Proactive. Health Promotion Uses an Interpersonal Approach in The Community to Increase Knowledge, Attitudes and Change People's Behavior.
Keywords: Accessibility; Anxiety; Health Center; Immunization; knowledge

Immunomodulatory and Acute Toxicity Tests of Rhizome Ethanol Extract of Etlingera Flexuosa Poulsen (Zingiberaceae) on Male Mice (Mus Musculus) - Ramadanil Pitopang; Nadhirah Nur Azizah Lubis; Mifthahul Jannah Tahapary Zubair; Puti Andalusia Sarigando Banilai; Nurhaeni; Ihwan (Pharmacognosy Journal, 2023)
Introduction: Immunomodulators are molecules of synthetic or biological origin that help to regulate the immune system. Many studies have focuses on exploring for phytochemical compounds that used as immunomodulatory properties in Indonesia, as well as in Sulawesi. The immunomodulatory activity of rhizome extract of E. flexuosa, an endemic flowering plant of Sulawesi on male mice were studied. Methods: 25 male mice (Mus musculus) used were randomly divided into 5 groups and Staphylococcus aureus (ATCC 25923) was used as inducer. The negative control group was given 0.5% Na-CMC (Carboxymethyl Cellulosa Sodium), positive control group was given stimuno® and treatment groups were an ethanol extract of E. flexuosa with successive doses of 200, 400 and 800 mg/kg body weight (BW) respectively. Each group was given the preparation orally for 7 days and on the 8th day the test animals were induced by Staphylococcus aureus bacteria intraperitoneally. The mice were dissected and the peritoneal fluid was taken to determine the activity of the macrophage cells. Meanwhile, Thomson and Weil method was used to study the acute toxicity test and determine the lethal dose 50 (LD50). Results: The percentage of macrophage activity in each group of negative control, positive control, extract doses of 200, 400 and 800 mg/kg BW respectively were 40.40%, 82.65%, 53.05%, 69.38% and 82.06%. Based on the results obtained, it was shown that the E. flexuosa rhizome extract has an optimum dose of 800 mg/kg BW, which was not significantly different from the positive control. Meanwhile, the symptoms of toxicity began to appear from a dose of 600 mg/kg BW to a dose of 2400 mg/kg BW including decreased motor activity, tremor, ataxia, lids and writhing. LD50 expressed in LD50 within the criteria of being practically non-toxic. Conclusions: The E. flexuosa rhizome ethanolic extract showed the immunomodulatory activity at optimum dose of 800 mg/kg BW by the increasing of macrophage phagocytosis activity. Moreover, the extract was also practically non-toxic based on LD50 value.
Keywords: Etlingera flexuosa; Immunomodulators; Immunostimulants; Lethal Dose 50; Macrophages; Phagocytosis; Toxicity

Immunomodulatory and Antiallergic Potentials of the Bioactive Compounds of Ginger - B. Lalruatfela; P. B. Lalthanpuii; C. Lalrinmawia; K. Lalchhandama (Pharmacognosy Journal, 2023)
Background: Allergy is an ever-increasing immune disorder and is often fatal under certain circumstances. Lack of total curative medication prompts the search for various compounds as the lead molecules. Ginger, Zingiber officinale Roscoe, is a well-established medicinal plant in different traditional practices. Its use as antiallergic or anti-inflammatory agent has been vindicated but the underlying mechanism of action is yet unknown. Method: In this study, we analyzed the phytocompounds characterized from ginger for their binding affinities on cysteinyl leukotriene receptor 1 (CysLTR1) and histamine H1 receptor (H1R) by molecular docking. The molecular interactions were compared against known agonists and antagonists of the two receptors. Results: The data indicate that ginger compounds have high binding affinity for both LTR1 and H1R comparable to those of antiallergic medications. The highest binding affinities were recorded for gingerenone-A (-7.3 kcal/mol) and zingiberol (-7.2 kcal/mol) on LTR1; and gingerenone-A (-8.7 kcal/mol) and α-curcumene (-8.0 kcal/mol) on H1R. Conclusion: In addition to antiallergic activity, molecular predications on the probable biological activities of the ginger compounds show that they can have a variety of medicinal applications including immunomodulatory and anticancer activities.
Keywords: Allergy; Ginger; Histamine Receptor; Leukotriene Receptor; Molecular Modelling

Immunostimulating Activity of Sungkai Leaf Stigmasterol Isolate against Cluster Differentiated 8+ T (CD8+T) from Covid 19 Disease Exposure - Dwisari Dillasamola; Fatma Sri Wahyuni; Rauza Sukma Rita; Yufri Aldi (Pharmacognosy Journal, 2023)
Objective: Covid 19 is a disease that infects cells in the airway lining the alveoli. This disease is caused by a virus named SarsCoV2. One way to handle it is to increase immunity. The body itself consists of immune system organs. One component of the immune system that is very important in protecting the body against the SARS-Cov-2 virus attack is Cluster of Differentiation 8+ T (CD8+T). CD8+T is one of the components of the adaptive immune system or often known as the specific immune system and is cytotoxic, so it is called Cluster of Differentiation8 +T. One of the plants used by the community to increase endurance is Sungkai (Peronema canescens Jack.). This study aims to see its activity against Cluster of Differentiation 8+ T (CD8+T). Methods: Sungkai leaf powder was extracted using 70% ethanol solvent and evaporated using a rotary evaporator to obtain ethanol extract and fractionated with n-hexanes and isolated to obtain active isolates of sungkai leaves against the immune system. Isolates obtained from the isolation of the active fraction of n hexanes that have been tested as immunostimulants in previous studies. This study used test animals, namely male white mice of the wistar strain as many as 30 mice consisting of 5 heads for each group. Group I is a control group that is not given treatment and groups II, III, IV, V and VI are given exposure to the Sars CoV2 virus antigen that causes Covid 19 disease, namely using one of the Covid19 vaccines, namely the 0.0013 mL Moderna vaccine which is given Intra Muscular. Groups II, III, IV, V are treatment groups induced with Sars CoV2 antigen and given a test preparation, namely sungkai leaf isolate with 3 dose variations, namely 1, 10 and 100 mg/kg bw given orally, and group VI was given stimuno 50 mg/kgbw as a comparison. Results: From the results of immunostimulant testing of sungkai leaf isolate, it was found that stigmasterol has an immunostimulant effect in increasing Cluster of Differentiation 8 + T (CD8 + T) in the body given SARS-CoV-2 virus antigen. Significant results were seen in the 100 mg/kg bw dose group which increased CD8+T by 53.93 ng/dl. Based on statistical tests conducted in the form of ANOVA tests, CD8+T also showed significant differences with a significance value of <0.05. Conclusion: Stigmasterol isolate of sungkai leaf has the effect of increasing Cluster of Differentiation 8+ T (CD8+T) levels in the body given exposure to SARS-CoV 2 virus antigens.
Keywords: Cluster of Differentiation 8+ T (CD8+T); Immunostimulant.; SARS-CoV-2; Stigmasterol; Sungkai

The Impact of Kaempferia galanga L. Extract on Nerve Growth Factor Expression in A Rat Model of Traumatic Brain Injury - Muhammad Fauzan Jauhari; Lucia Yovita Hendrati; Agus Turchan; Asra Al Fauzi; Budi Utomo; Eko Agus Subagio (Pharmacognosy Journal, 2023)
Introduction: Head injuries contribute significantly to morbidity and mortality globally, and there is need of effective treatment. This study focuses on evaluating NGF (Nerve Growth Factor) expression in a rat model of traumatic brain injury, exploring the potential therapeutic effects of Kaempferia galanga L. extract. Methods: Male Wistar rats were used in the experiment, and traumatic brain injury was induced using Marmarou's weight drop model. Four groups of rats were studied: a negative control group, a group with traumatic brain injury without Kaempferia galanga L. extract, and two groups with traumatic brain injury treated with different doses of Kaempferia galanga L. extract. Rats were divided further based on the time of decapitation, either 24 or 48 hours post-injury. NGF expression was assessed using immunohistochemistry. Results: The study confirmed NGF expression variations among groups, with stronger expression observed 48 hours post-injury in rats receiving 1200 mg/kgbb of Kaempferia galanga L. extract. This suggests a potential impact of the extract on NGF expression, likely attributed to its anti-inflammatory and antioxidant properties. Discussion: Kaempferia galanga L. extract has known anti-inflammatory and antioxidant effects, which may contribute to increased NGF expression observed in this study. Conclusion: This study sheds light on the potential benefits of Kaempferia galanga L. extract in promoting NGF expression and improving outcomes in traumatic brain injury, emphasizing the need for further investigation to translate these findings into clinical practice.
Keywords: kaempferia galanga; Nerve Growth Factor; Traumatic Brain Injury

Implementation Analysis of COVID-19 Vaccination Policy in Southwest Maluku Regency - Joska Rap Rap; Balqis; Sukri Palutturi; Indar; Masni; Atjo Wahyu; Anwar Mallongi (Pharmacognosy Journal, 2023)
The implementation of the COVID-19 vaccination policy can be influenced by several factors, including communication, available resources, task disposition, and organizational structure. This study aims to analyze the factors affecting the implementation of these policies. This study employed a qualitative descriptive design, with data collection techniques including observation and interviews. The research was conducted in Southwest Maluku Regency in October-November 2022, with a total of 5 informants consisting of key informants, such as the Regent of Southwest Maluku as the chairman and spokesperson of the Task Force, ordinary informants, such as the Head of the Regional Disaster Management Agency, the head of Surveillance and Immunization at the District Health Office of Southwest Maluku, and the Coordinator of the COVID-19 Task Force Expert Team, as well as ordinary informants, such as community leaders. The results of the study revealed that the implementation of the COVID-19 Vaccination Policy in Southwest Maluku Regency has been well executed. The government has actively conducted health promotion and socialization, and has adequate resources equipped with specific competencies and expertise. However, there were several challenges during the implementation, such as the spread of misinformation, the difficult geographical terrain, and limited communication access to all areas. The findings of this study are expected to serve as an evaluation material for the government in the implementation of the COVID-19 vaccination program.
Keywords: COVID-19 Vaccine; Health promotion; Policy; Task Force.; Vaccination Policy

Improving the Quality of Assessment and Diagnosis in Nursing Care: A Literature Review - Brajakson Siokal; Ridwan Amiruddin; Tahir Abdullah; Yuliana Syam; Sukri Palutturi; Hasnawati Amqam; Rian Adi Pamungkas; Wahyuningsih; Samsualam; Sudarman; Akbar Asfar; Andi Mappanganro (Pharmacognosy Journal, 2023)
Nursing care involves a series of interactions between nurses with clients and the environment to meet their needs and ensure independence in caring for themselves. Therefore, this research aimed to examine how to improve the quality of assessments and diagnoses in nursing care. Several articles were obtained from Google Scholar, Pubmed, and ProQuest databases, while the research questions were structured using the PICO method and search keywords. The inclusion criteria included articles focused on the quality of assessments and diagnoses in nursing care, applied to health institutions and services, and published in English from 2018-2022. A total of six articles were included after thorough identification, screening, eligibility, and inclusion. The results obtained can be used to improve the quality of assessments and diagnoses in nursing care.
Keywords: Nil

In silico ADME and Drug-likeness Evaluation of Phytochemicals from the Leaves of Tabernaemontana divaricata Linn. - Ayda Cherian; Velmurugan Vadivel (Pharmacognosy Journal, 2023)
Introduction: Tabernaemontana divaricata is a traditional plant from the family of Apocynaceae, which has wider medicinal activities such brain tonic, anti-epileptic, anti-mania and anti-oxidant. The current predictive study was aimed to know pharmacokinetics and drug likeness of selected phytochemicals present in T.divaricata by using online tool Swiss-ADME. Methods: The air-dried leaves were pulverized and subjected to Soxhlet extraction and percolation using the solvents, namely, ethanol, hydroalcoholic solvent (50:50 and 70:30 ethanol: water) and water to obtain four different extracts. Aqueous extract was made through percolation. Subsequently, gas chromatography-mass spectrometry was used to analyze each extract further. All the bioactive compounds were subjected to in silico ADME and drug-likeness studies and the finalized compounds were undergone cell cytotoxicity activity. Results: All the four extracts have distinct physicochemical properties linked to the chemicals naturally present in large amounts in T. divaricata leaves. The compound 4,4,7a-Trimethylhexahydro-1-benzofuran-2(3H)- one and 2-(4-methylphenyl) indolizine having good drug likeness of 4.50 and 3.50 respectively and good lipophilicity which has the log P value of 2.51 and 3.73 appropriately. IC50 values of compounds were found to be 312.1 ± 0.2μg/ml for 4,4,7a-Trimethylhexahydro-1-benzofuran-2(3H)-one and 393.7 ± 0.2μg/ ml for 2-(4-methylphenyl) indolizine. Conclusion: Major bioactive chemicals were found in the aqueous extract and based on the calculated ADME parameters they are anticipated to serve as cytotoxic lead compounds. It is advocated that current predictive results should be authenticated by in vitro and in vivo toxicological and pharmacological assay.
Keywords: Apocyanceae; Cell cytotoxicity studies; GC-MS analysis; In silico study

In Silico Study of Rhamnocitrin Extract from Clove Syzygium Aromaricum in Inhibiting Adenosine A1 Adenylate Cyclase Interaction - Nita Yessirita; Rismi Verawati; Devi Purnamasari; Rollando Rollando; Riso Sari Mandeli; Muhammad Thoriq Albari; Putri Azhari; Rahadian Zainul; Viol Dhea Kharisma; Vikash Jakhmola; Maksim Rebezov; ANM Ansori (Pharmacognosy Journal, 2023)
This study aims to analyze the potential of Rhamnocitrin, a compound found in clove extract (Syzygium aromaticum), as an inhibitor of Adenylate Cyclase through an in-silico approach. The research method involves the use of software such as Pymol, PyRx, Protein Plus, and Lipinski Rule for molecular interaction analysis and physicochemical characterization of Rhamnocitrin. The analysis results show that Rhamnocitrin has significant affinity towards Adenosine A1 with Binding Affinity values of -6.1, -5.8, and -5.7. RMSD analysis indicates good stability of the formed protein-ligand complexes, with RMSD values of 0, 3.129, and 3.696. Analysis using Protein Plus software reveals the interaction between Rhamnocitrin and Adenosine A1, while the lipinski analysis shows physicochemical characteristics of Rhamnocitrin that meet important criteria, such as a mass of 300, 3 hydrogen bond donors, 6 hydrogen bond acceptors, log P of 2.6, and molar reactivity of 77.27. These findings provide new insights into the development of potential therapies involving clove extract and Rhamnocitrin as inhibitors of Adenylate Cyclase, and further research is needed to validate their effectiveness and safety.
Keywords: Adenosine A1; Adenylate Cyclase inhibition; Molecular Docking.; Rhamnocitrin; Syzygium aromaricum

In Silico Study on the Inhibition of Sitogluside from Clove Plant (Syzygium aromaticum) on Interleukin 2 in B and T Cell Proliferation - Linda Rosalina; Devi Purnamasari; Rismi Verawati; Okta Suryani; Muhammad Arya Ghifari; Amalia Putri Lubis; Rahadian Zainul; Riso Sari Mandeli; Viol Dhea Kharisma; Vikash Jakhmola; Maksim Rebezov; ANM Ansori (Pharmacognosy Journal, 2023)
This research discusses an in-silico study of sitogluside found in the clove plant (Syzygium aromaticum) as a potential inhibitor of B and T cell proliferation through interaction with Interleukin-2. This study utilizes methods such as Swiss Target Prediction, Pymol, Pyrex, Protein Plus, and Lipinski's Rule to predict the biological activity and pharmacokinetic characteristics of sitogluside. From the docking simulation results, sitogluside exhibited strong interactions with interleukin-2 with RMSD values of 0, 1.637, and 2.299, and Binding Affinities of -5.7, -5.5, and -5.5, indicating its potential effectiveness as an inhibitor. In addition, sitogluside fulfills Lipinski's rule with a molecular mass of 520, 4 hydrogen bond donors and acceptors, a log P value of 2.3, and a molar reactivity of 133, indicating a high potential for good bioavailability in biological systems. These results suggest that sitogluside from the clove plant holds potential as a new therapy in inhibiting B and T cell proliferation, however further research is needed to validate these findings and explore its potential in clinical treatments.
Keywords: Cell Proliferation; Interleukin-2; Molecular docking; Sitogluside; Syzygium.

In Silico Study on the Potential of Guaiacol Extract from Green Tea (Camellia sinensis) as a Stimulant for Carbanoic Anhydrase II in Renal Tubular Acidosis - Rahadian Zainul; Rismi Verawati; Agus Suprijono; Riso Sari Mandeli; Asri Peni Wulandari; Dony Novaliendry; Ritmaleni; Linda Rosalina; Muhammad Arya Ghifari; Amalia Putri Lubis; Viol Dhea Kharisma; Vikash Jakhmola; et al. (Pharmacognosy Journal, 2023)
This study explores the potential of Guaiacol, a green tea extract from Camellia sinensis, as a stimulant in renal tubular acidosis through in-silico investigation on the Carbanoic Anhydrase II enzyme. Utilizing comprehensive computational tools including PyMOL, PyRx, Protein Plus, and the Lipinski's Rule of Five, a detailed examination of the molecular structure and its interactions with the target enzyme was conducted. The results from Protein Plus revealed interactions between Guaiacol and Carbanoic Anhydrase II. Quantitative parameters were determined with Binding Affinity values of -5, -4.7, and -4.5, along with RMSD values of 0, 0.956, and 1.412. The Lipinski's Rule of Five was employed to evaluate the compound's drug-like properties, with the findings indicating a molecular weight of 124, one hydrogen bond donor, two hydrogen bond acceptors, a log P of 1.4, and a molar reactivity of 34.65. Overall, these findings suggest that Guaiacol holds promising therapeutic potential in the treatment of renal tubular acidosis.
Keywords: Camellia sinensis.; Carbanoic Anhydrase II; Guaiacol; Molecular docking; Renal Tubular Acidosis

Inflammatory Thyroid Changes Following Serotonin Receptor Blocking in Experimental Rats - Muthear N Dawood; Karam A. Aldabbagh; Zahraa Alsarraf (Pharmacognosy Journal, 2023)
According to studies, a pathophysiological feature of schizophrenia may be a dysregulation of the inflammatory immune response. Conversely, antipsychotic medications have been found to have an immunosuppressive effect in patients with schizophrenia; however, this has not been consistently observed in different studies. The purpose of the following study is to compare the effects of risperidone with aripiprazole on thyroid function as it relates to inflammatory markers (CRP). This study was based on a randomized controlled trial. A total of thirty rats were recruited for the experiment and were kept in the artificial, and optimal environment. The rats were divided into three groups; each group has an equal number of rats which was 10 rats each. The first group was the control group which received the placebo, in the second group, there were 10 rats too, which was known as the risperidone group. Each rat received 20mg/kg/day through I/V. The third group is known as the aripiprazole group which received the drug from the intravenous route, 10mg/kg//day. In the results, the summarized values represented that all the mean values before and after the treatment remained less than 3.0. From the results and other evidence, it can be said that although the subjects who receive the following results do not require regular or frequent monitoring of thyroid hormones in long-term use and in the use of the drug in higher concentration there must be a check as long term use is associated with hyperthyroidism.
Keywords: Aripiprazole; FT3; FT4; Risperidone.; Thyroid; Thyroxine; Tri-iodothyronine

The Influence of Effective Nurse Communication Application on Patient Satisfaction: A Literature Review - Brajakson Siokal; Ridwan Amiruddin; Tahir Abdullah; Yahya Thamrin; Sukri Palutturi; Erniwati Ibrahim; Yuliana Syam; Rian Adi Pamungkas; Samsualam; Andi Mappanganro; Wahyuningsih; Anwar Mallongi (Pharmacognosy Journal, 2023)
Communication is an important element for building trust and comfort in nursing, and it is the foundation of the nurse-patient relationship. The purpose of this study is to find out how effective communication influences nurses' perceptions of patient satisfaction. A recent literature review was conducted using the Pubmed, Wiley Online, and Science Direct electronic databases. The review method uses the terms and expressions of the keywords "effective communication," "nurse," and "patient satisfaction." Based on the screening results for inclusion and exclusion criteria, a literature search turned up 10 studies. Effective communication provided by nurses greatly affects patient satisfaction; the better effective communication is carried out, the greater the patient satisfaction will be. With patient satisfaction, nurse communication can improve service by creating good cooperative relationships and fostering trust so as to improve patient recovery. Not only with patients, but effective communication must also be carried out with colleagues and other professions by applying several methods such as ISBAR, PCC, and MCA communication methods. Therefore, health services, especially nurses, should pay more attention to effective communication with patients and implement this effective communication in nursing care activities.
Keywords: Effective communication; Nurses; Patient satisfaction.

The Influence of Job Satisfaction and Organizational Commitment on Nurse Performance with Work Motivation as a Mediating Factor at I Lagaligo East Luwu Hospital In 2022 - Maria Ulfha Ibrahim; Alimin Maidin; Irwandy; Indahwaty Sidin; Fridawaty Rivai; Khalid Shaleh (Pharmacognosy Journal, 2023)
Nursing performance is an important thing that needs to be studied in order to maintain and improve the quality of hospital services. There are many factors that affect nurse performance. This study aims to analyze the effect of job satisfaction and organizational commitment on the performance of nurses with work motivation as a mediating factor at I Lagaligo East Luwu Hospital. The type of research conducted is quantitative research using an analytic observational study with a cross sectional study design. The sampling technique is proportional random sampling with the number of samples in this study being 130 nurses. Testing the hypothesis in this study using path analysis. The results showed that job satisfaction has a positive and significant effect on work motivation (β= 0,264; T statistic= 2,433). Organizational commitment has a positive and significant effect on work motivation (β= 0,347; T statistic= 3,742). Work motivation has a positive and significant effect on performance (β= 0,160; T statistic= 2,533). Job satisfaction has a positive and significant effect on performance (β = 0,179; T statistic= 2,510). Organizational commitment has a positive and significant effect on performance (β= 0,151; T statistic= 2,163). Job satisfaction has no direct effect on performance with work motivation as a mediating factor (β= 0,042; T statistic = 1,888). Organizational commitment has no direct effect on performance with work motivation as a mediating factor (β= 0,056; T statistic = 1,911). Hospitals are expected to be able to maintain job satisfaction, organizational commitment and work motivation of nurses so that every nurse is willing to provide the best productivity and performance for the realization of improving the quality of nursing services.
Keywords: Hospital.; Job satisfaction; Nurse performance; Organizational commitment; Work motivation

Influence of the Environment on Biofilm Formation Candida albicans of Vulvovaginal Candidiasis Isolate Patient - Wirda Anggraini; Djoko Agus Purwanto; Idha Kusumawati; Isnaeni3, Suryanto (Pharmacognosy Journal, 2023)
Context: Candida albicans is a type of fungus that can produce biofilms and may cause Vulvovaginal Candidiasis (VVC) disease. We investigated the effect of environment on biofilm formation of C. albicans patient isolates and ATCC 14053. Biofilm formation is influenced by several factors such as environments and nutrients. Objectives: To investigated the effect of environment on biofilm formation of C. albicans patient isolates and ATCC 14053. Methods: The samples using C. albicans ATCC 14053, C. albicans, which may form biofilms, was isolated from patient Dermatology and Venereology and Obstetrics and Gynecology from a hospital in Malang. TCP (A tissue Culture Plate) is the biofilm formation method used. Results: Biofilm formation took 48-72 hours at 25 °C and 96-120 hours at 37 °C. Based on the result biofilm formation of C. albicans is influenced by environmental factors and characterized by a high OD value. Conclusions: Biofilm formation is accelerated in temperature incubation needed at 25 °C for 48-72 hours, using biomass 107 CFU/mL, nutrition using Potato Dextrose Broth media and 1% glucose, and the solvent of 30% acetic acid to obtain acid condition.
Keywords: Biofilm formation; Candida albicans; sabourau dextrose broth; potato dextrose broth; vulvovaginal candidiasis

The Influence of Vitamin D3 Administration on the Levels of CGRP, Glutamate, and NLRP3 during the Ictal Phase in Chronic Migraine Patients - Restu Susanti; Yuliarni Syafrita; Afriwardi; Rauza Sukma Rita; Eryati Darwin; Nur Indrawaty Lipoeto; Hirowati Ali; Fika Tri Anggraini (Pharmacognosy Journal, 2023)
Background: Migraine is a primary headache disorder that ranks as the third leading cause of disability. Various prophylactic therapies have been developed for migraine treatment, including vitamin D3 supplementation. The mechanism of action of vitamin D3 supplementation in the pathophysiology of migraine has not been extensively studied. Objective: This study assesses the impact of vitamin D3 administration on the levels of the biomarkers CGRP, Glutamate, and NLRP3 and its effect on reducing the frequency and intensity of migraine attacks in chronic migraine patients. Methods: This experimental study (single-blind clinical trial) observes two groups: a group given vitamin D3 at 2000 IU for 12 weeks and a placebo group. This study involved 61 chronic migraine subjects. At the end of the study, after excluded 31 subjects, there were 12 subjects in each of the two groups. Results: A significant relationship was found between vitamin D3 administration and the reduction in the frequency and duration of migraine attacks in both the vitamin D3 group (p<0.001) and the placebo group (p=0.078). No significant relationship was found between vitamin D3 administration and changes in CGRP levels (p=0.633), but there were significant changes in glutamate (p<0.001) and NLRP3 (p=0.016) levels following vitamin D3 administration. Conclusion: Vitamin D3 supplementation has an impact on reducing the frequency and duration of migraine attacks in chronic migraine patients, and there is a significant association with changes in glutamate and NLRP3 levels, but not with changes in CGRP levels.
Keywords: CGRP; Chronic Migraine; Glutamate; NLRP3; Vitamin D3

The Influence of Work Engagement on Job Satisfaction and Human Resource Performance in South Sulawesi Provincial Hospital 2022 - Nurfhadila Utami Husain; Syahrir A Pasinringi; Fridawaty Rivai; Noer Bahry Noor; Khalid Saleh (Pharmacognosy Journal, 2023)
One of the most important factors for an individual to have at work is work engagement which is a concept that can reflect that an individual has passion, focus, and also strong dedication to work at the company. Work engagement is also defined as a positive and satisfying mental state related to work that is characterized by vigor, dedication, and absorption.1 An HR can be said to be work engagement when an HR is able to make his work a priority for himself and personally for the organization besides that work engagement is personal HR who are proud to have been part of an organization so that they are able to work according to the jobdesk given (Rohana Manalu et al., 2021). This study aims to analyze the effect of Work Engagement on Job Satisfaction and HR Performance at the South Sulawesi Provincial Hospital. This type of research is quantitative research using an analytic observational design with a cross sectional study approach. The sample in this study were health workers using a stratified random sampling technique of 316 people. The samples taken in this study were 152 employees at Laburan Baji Hospital, 122 Haji Hospital and 42 people at Sayang Rakyat Hospital. Data analysis used univariate, bivariate & path analysis. The results showed that there was a direct effect of work engagement on job satisfaction and HR performance with a coefficient of 0.525 and 0.267 for each with a p value (0.000) < 0.05 (significant). In addition, there is a direct effect of job satisfaction on HR performance, namely a coefficient value of 0.620 with a p value (0.000) <0.05. Meanwhile, the effect of work engagement on HR performance through job satisfaction obtained a coefficient value of 0.326 with a p value (0.000) < 0.05. which means Work Engagement has an influence on HR Performance through Job Satisfaction.
Keywords: Performance.; Satisfaction; Work engagement

Interaction of Cynaroside from Orthosiphon Aristatus Plant Extract on TNF Alpha as a Stimulant in Malaria and Asthma - Rahadian Zainul; Rismi Verawati; Gemini Alam; Khoirun Nisyak; Trisna Kumala Sari; Muhammad Arya Ghifari; Ritbey Ruga; Putri Azhari; Romadhon; Himmatul Barroroh; Riso Sari Mandeli; Devi Purnamasari; et al. (Pharmacognosy Journal, 2023)
This research aims to investigate the interaction between cynaroside, a natural compound found in Orthosiphon aristatus plant extract, with TNF Alpha as a stimulant in the context of malaria and asthma. The research method involved an in-silico approach using software such as Pymol, PyRx, Protein Plus, and the Lepinski Rule. The results of the study showed that cynaroside has a significant interaction with TNF Alpha, as indicated by high Binding Affinity values of -9.6, -9.3, and -9.2. Analysis using Protein Plus confirmed the interaction between cynaroside and TNF Alpha. Additionally, evaluation using the Lepinski Rule of Five revealed that cynaroside has physicochemical characteristics suitable as a potential drug compound, with a mass of 448, hydrogen bond donors of 7, hydrogen bond acceptors of 11, log p -0.401, and molar reactivity of 105.2. These findings provide a deeper understanding of the potential of cynaroside in regulating the immune response to malaria and asthma through its interaction with TNF Alpha. These results can serve as an important basis for further research in the development of more targeted and effective therapies for both of these diseases
Keywords: Asthma.; Cynaroside; Malaria; Molecular docking; Orthosiphon aristatus; TNF Alpha

Interaction of Masilinic Acid from Clove Plant (Syzygium aromaticum) with CD81 Antigen in Inhibiting HIV Virus Regulation In Silico - Rahadian Zainul; Elsa Yanuarti; Siti Amiroch; Muhammad Thoriq Albari; Rismi Verawati; Amalia Putri Lubis; AAA Murtadlo (Pharmacognosy Journal, 2023)
This research explores the interaction of Masilinic Acid from the clove plant (Syzygium aromaticum) with the CD81 antigen to inhibit HIV virus regulation in silico. Using computational methods such as Pymol, Pyrex, and Protein Plus, we demonstrate that Masilinic Acid can significantly interact with the CD81 antigen. The obtained data shows binding affinities of -6.4, -6.2, and -5.7, and RMSD values of 0, 1.885, and 1.952. Further detailed interaction analysis with Protein Plus strengthens these findings, providing evidence of a strong interaction between Masilinic Acid and the CD81 antigen. This study also includes the testing of the Lepinski Rule of Five to assess the potential of Masilinic Acid as a drug candidate, with results indicating a mass of 472, three hydrogen bond donors, four hydrogen bond acceptors, a log P value of 6.2, and a molar reactivity of 134. These results indicate that Masilinic Acid has the potential as an inhibitor of the CD81-HIV interaction, which can be utilized as an effective antiviral strategy. Key words: Masilinic Acid, Clove plant, CD81 antigen, HIV virus, In silico.
Keywords: CD81 antigen; Clove plant; HIV virus; In Silico.; Masilinic Acid

Interpolation Pedicle Flap Technique in Wound Defect with Tibial Bone Exposed: A Case Report - Zainarda; Andi Asadul Islam; Muhammad Sakti (Pharmacognosy Journal, 2023)
Interpolation flap is a flap constructed from nonadjacent donor tissue that carry an inherent blood supply (vascular pedicle). They are ideally suitable for reconstruction of large or deep defect. These flaps have their own blood supply therefore they are more likely to survive on exposed bone or cartilage. The use of these technique should result in a bridge of tissue, or pedicle, between the flap base and the surgical defect. A 61-years-old man admitted to the emergency unit 2 hours ago after a traffic accident. Physical examination shows crush injuries on the right lower limb with open wounds exposing the muscles, tendons, and bone. In the distal neurovascular (NVD) obtained capillary refill time < 2 seconds, limited active movement due to pain, and good sensory. After the initial treatment of external fixation and debridement, a week follow up found a wound defect on the medial side of the leg around 10 x 15 cm with tibial bone exposed as a base of wound defect. The usage of Pedicle Interpolation Flap Technique is a better choice to delayed treatment for soft tissue defect with bone exposed. The anatomy of artery is essential as a requirement to obtain the flap needed. Fine and holistic care of the flap shall increase the success rate of regaining and retaining anatomical and physiological, as well as cosmetic, and exponentially lower the probability of complications occurrence by accurate planning and hand in hand care to better strength, motion and functional outcome.

Intervention of Giving Moringa Biscuits (Moringa Oliefera) Mix Sori Fish Flour to Increased Blood Hemoglobin Levels in Young Girls, Kendari, INDONESIA - Fatmawati; Suriana Koro; Nadimin; Kameriah Gani; Hasan; Ellyani Abadi; Anwar Mallongi (Pharmacognosy Journal, 2023)
Adolescents are the next generation and national development assets, one of the nutritional problems in adolescents is anemia, namely the lack of hemoglobin levels below normal (12 mg/dl). Efforts to deal with anemia are making food in the form of Moringa biscuits and sori fish which are rich in nutrients. The purpose of this study was to determine the effect of giving Moringa mixed sori biscuits on blood hemoglobin levels in adolescents. The type of research is a quasi-experimental conducted in June- September 2021 at Coastal Region Public Middle Schools throughout Kendari City in 2021, namely at SMPN 4 Kendari, SMPN 5 Kendari, SMPN 10 Kendari, SMPN 15 Kendari and SMPN 16 Kendari. The sample is anemic adolescent girls in the coastal area of Kendari City. The intervention sample was anemic adolescent girls who were given moringa biscuits mixed with sori fish flour as many as 36 people and the control sample was anemic adolescent girls who were not given moringa biscuits mixed with sori fish flour as many as 36 people. The case sampling technique used cluster random sampling and the control sample used matching. Data collection of blood hemoglobin levels using a portable device brand Easy Touch Meter. Data were analyzed using independent t-test test. The results of the study found blood hemoglobin levels before and after Moringa biscuits were given, namely in the case group before the intervention the average Hb level reached 10.51 mg/dl and after the intervention it reached 12.45 mg/ dl, while in the control group before the intervention it was 12.79 mg/dl and after intervention 13.49 mg/ dl. The results of the independent t-test obtained p value 0.000. The conclusion is that there is an effect of giving Moringa fish biscuits mixed with Sori fish flour on hemoglobin levels. Suggestions for young women are expected to increase the intake of protein sources of nutrients such as fish, eggs, tofu and tempeh and iron (Fe) which can be obtained from green vegetables such as Moringa to prevent and treat anemia.
Keywords: Adolescents.; Blood Haemoglobin; Moringa Biscuits; Sori Fish

Intravitreal Resveratrol as Anti Apoptotic Agent Against Retinal Ganglion Cell Loss in Ischemic Reperfusion Injury - Amelia Shinta Prasetya; Evelyn Komaratih; Wimbo Sasono; Mercia Chrysanti; Maria Debora Niken Larasati; I Ketut Sudiana (Pharmacognosy Journal, 2023)
Background: Glaucoma is an optic neuropathy caused by the apoptosis of retinal ganglion cells and results in progressive retinal ganglion cell injury. A decrease in intraocular pressure (IOP) is a modifiable risk factor for slowing the progression of the disease, and can be accomplished through medication, laser therapy, or surgery. Even though the intraocular pressure has decreased and attained normal levels, the injury to the retinal ganglion cells continues in some cases. It is believed that neuroprotective administration has a positive effect on preventing the loss of retinal ganglion cells. Methods: Bax and Caspase-3 expression were measured involving 20 eyeballs of Rattus Norvegicus by immunohistochemistry examination. I-R injury was developed by increasing intraocular pressure (IOP) through the intracameral balanced salt solution (BSS) injection, then lowered after 60 minutes. Samples were divided into 4 groups: control, no further injection group, phosphate-buffered saline (PBS)-injected group and resveratrol-injected group. Each group was enucleated at days 7, 0, 7, and 7, respectively. Data with a non-normal distribution were examined using the Kruskal-Wallis test, and if the outcome was significant, the Mann-Whitney test. Results: The highest mean Bax and Caspase-3 expression was found in PBS injected and enucleated at day 7 group (G2), 0.96±0.40 and 0.72 ± 0.30, respectively. When compared to PBS injection, the expression of Bax and Caspase-3 was lower in the resveratrol-injected group. Conclusion: Bax and Caspase-3 expressions were lower in the intravitreal injection of Resveratrol in the dose of 100 µM following the I-R injury group compared to the group without intravitreal Resveratrol injection.
Keywords: Apoptosis; Glaucoma; Ischemic-reperfusion injury; Neuroprotective; Resveratrol