The present study focused to evaluate the antibacterial and antifungal activity of various fruit seeds extracts against certain bacterial and fungal stains, using cup plate diffusion method. Around 24 seeds were selected for the study and extracted using suitable solvent system. The ethanolic extracts were subjected to assess the antimicrobial activity. The results suggest that the ethanolic extracts of Banana, pineapple and musambi showed no significant activity against bacterial and fungal strains. Jackfruit and papaya extracts shown similar activity against both gram positive and gram negative bacterial strains but papaya shown better antifungal activity. Potential antibacterial activity was observed for tamarind and guava extracts, tamarind also exhibits better antifungal activity. Among all the extracts plum seed extract shown potential broad spectrum antimicrobial activity. The study can be concluded that fruit seed extracts may find better application in cosmeceutical preparation especially in body deodorant sprays.