In vitro Antioxidant Activity of Moringa pterigosperma (Gaertn) leaves.
The human body produces reactive oxygen species (ROS) as a result of normal metabolic process. These ROS are capable of oxidizing biomolecules that can damage DNA, cells and contribute to chronic disease. In treatment of these diseases, antioxidant therapy is gained an utmost importance. The important role of dietary antioxidants maintaining the integrity of the living organisms is gaining ever increasing recognition. The oxidative stress induced due to ROS can be attenuated or perhaps reversed by diets containing vegetables that have an ability to scavenge reactive oxygen species. Moringa pterigosperma (Gaertn) leaves has been used in Indian cooking and herbal remedies. Its possible mechanism of action was examined in terms of antioxidant availability. The study was designed to evaluate and compare the antioxidant activity, total phenolics, flavonoids content of aqueous (AEMP) and ethanolic extract (EEMP) extracts of leaves. The antioxidant activity was assessed by DPPH (1, 1–diphenyl–1,2–picryl hydrazyl), nitric oxide and superoxide radical scavenging assay, FRAP (Ferric Reducing Antioxidant Power), reducing power and TAC (Total antioxidant capacity). AEMP has shown higher antioxidant activity as compared to EEMP in DPPH radical scavenging assay with IC50 values 3649.63±1.81 in AEMP and 3048.78±1.23 in EEMP respectively. Like antioxidant activity the reducing power and FRAP values of AEMP are better as compared to EEMP. The amounts of total phenolic and flavonoid content were also determined. In conclusion, the studies reveal that Moringa pterigosperma Gaertn. can scavenge radicals and reduce iron complex may explain the possible mechanism by which it exhibits beneficial effects.