Chuquiraga spinosa Lessing : A Medicinal Plant for Gastric Cancer Induced By N-Methyl-N-Nitroso-Urea ( NMU )

Background: Gastric cancer (GC) is one of the most frequent diseases in human population: Many plants from Peruvian flora is used to treat cancer as alternative treatment. Chuquiraga spinosa Lessing (ChS) is a species with high potential therapeutic due to its antioxidant and anti-inflammatory effect as well as protective against prostate cancer. Objective: The main objective was to evaluate the possible protective effect of Chuquiraga spinosa extract on NMU (N-methyl-N nitrosourea)-induced gastric cancer in rats. Methods: Gastric carcinogenesis was induced in 30 male Holtzman rats by providing NMU 50 μg/Kg by oral administration for 16 weeks. Ethanolic extract of ChS aerial parts was administered at doses 50, 250 and 500 mg/Kg per oral. The protective effect was determined through weight controls, biochemical and hematological parameters; the antioxidant capacity by superoxide dismutase (SOD), nitric oxide (NO), malondialdehyde (MDA) and anti-inflammatory capacity by the level of C-reactive protein (CRP). The tumors were monitored by using histological examinations. Results: Oral administration of Chuquiraga spinosa extract significantly decreased superoxide dismutase malondialdehyde, nitric oxide, C-reactive protein levels (p<0,01, p<0,01, p<0,01 and p<0,01 respectively compared with Inductor group). There was a significant increase in the weights of animals (P<0.05). Conclusion: Considering the anti-inflammatory, antioxidant, and anticancer properties of Chuquiraga spinosa extract, we conclude that it has a protective effect on NMU induced gastric cancer in rats.

Many medicinal plants are used by patients with cancer due to numerous positive effects on pain relief, vomiting, nauseas, depression, and anxiety. 8ioactive compounds such as polyphenols, tannins, flavonoids can have antioxidant capacity with potential benefits for health and could reduce cancer risk, it has been demonstrated that the anti-inflammatory effect of these compounds inhibit arachidonic acid (AA), prostaglandins, and leukotrienes. 9Polyphenols are chemoprotectives that act by blocking of initiation stage of carcinogenesis, free radicals and activation of glutathione peroxidase, glutathione reductase and inactivation of glutathione S transferase. 10huquiraga spinosa (ChS) Lessing (Family: Asteraceae) is called "huamanpinta" and is a species with therapeutic properties from Peruvian flora.The stem and leaves infusion of this plant is used for its antiinflammatory effects and urinary infections.Previous studies have reported that ChS presents anti-inflammatory and antimicrobial effects. 11The main objective in this research was to determine the protective effect of aerial parts of Chuquiraga spinosa on NMU induced gastric cancer in rats.

Animals
A total of 30 rats Holtzman (150 ± 20 g) of male sex were obtained from the National Institute of Health (Lima, Peru).Rats were kept in plastic cages with access to pelletized food and water ad libitum and housed in animal room with controlled temperature (22-24 °C) and 12 h light/dark cycle.Rats were divided into five groups of six animals each and acclimatized to the laboratory 15 days previous to the experiments.All experimental procedures involving animals were conducted in accordance to the guidelines established by the European Union on Animal Care (CCE Council 86/609) and approved by the Institute for Ethics in Health of the National University of San Marcos (01414-R-12-UNMSM).

Plant material
Chuquiraga spinosa was collected, in January, 2016 from Huancayo, Peru, and identified by Hamilton Beltran.A voucher specimen (152-USM-2016) was deposited at the National Herbarium of National University of San Marcos (UNMSM), Lima, Peru.

Extraction of plant material
The aerial parts of Chuquiraga spinosa (1000 g) were dried at room temperature and pulverized at the Chemical Laboratory, Faculty of Pharmacy and Biochemistry, Universidad Nacional San Luis Gonzaga de Ica (UNICA), the powder material was exhaustively soaked with 96 % ethanol and intermittent shaking every day for 7 days.The extract was filtered and evaporated by using a rotavap.The crude ethanolic extract obtained (20 g) was stored until further studies.

Phytochemical Screening
The presence of phytochemical constituents, such as alkaloids, terpenoids, quinone, flavonoids, tannins, saponins, steroids and phenolic compounds were determined with the standard qualitative phytochemical methods described. 12otective effect on NMU induced gastric cancer in rats Tumor induction In all groups, except negative control and NMU groups, tumor induction was carried out by following the method of Tatematsu et al. with brief modifications. 13NMU was used as carcinogenic agent for gastric cancer induction.Animals received a single dose of 500 μg/Kg of NMU dissolved in saline solution pH 5.0 by oral administration.

Experimental design
30 males Holtzman rats were divided into five groups of 6 rats each.Groups were placed according to the treatment and dose in mg/kg.ChS refers to Chuquiraga spinosa ethanolic extract.The negative control group received polysorbate 80 3% orally for 16 weeks.Group NMU was induced with NMU on the first day and received polysorbate 80 3% and NMU 500 ug/Kg orally for 16 weeks.The groups NMU + ChS 50, NMU + ChS 250, and NMU + ChS 500 received Chuquiraga spinosa (50, 250, and 500 mg/kg body weight, respectively) orally for 16 weeks with NMU induction.At the end of the experiment, rats were weighed.Blood samples were collected to assess the biochemical parameters and hematological indicators.The animals were sacrificed by using pentobarbital anesthesia (100 mg/kg).The protective effect was determined through weights as well as biochemical, hematological; the antioxidant capacity by superoxide dismutase (SOD), 14 nitric oxide (NO), 15 malondialdehyde (MDA) 16 and the anti-inflammatory capacity by the level of C-reactive protein (CRP). 17istological analysis.Stomach were removed and fixed in 10% formalin, processed, and embedded into paraffin blocks.Sections were cut at 5 µm thickness and stained with H&E (hematoxylin and eosin).The slides were examined under a light microscope (Olympus BX51).

Statistical analysis
Data are presented as mean ± standard deviation (SD).Data were analyzed for homogeneity of variance by the Levene test and normality by the Wilk-Shapiro W statistics. One-way analysis of variance (ANOVA) followed by Tukey multiple comparison method was carried out to compare the mean value of different groups.A P-value of 0.05 was considered statistically significant in all cases.Data were analyzed by using SPSS v. 21.

Ethical considerations
During the experimental process, international ethical principles for research using laboratory animals were respected.The rats were euthanized by intravenous injection of pentobarbital (100 mg/kg).This method leads to a quick and peaceful death, acceptable for rats.

RESULTS
Table 1 shows the secondary metabolites present in the ethanolic extract of aerial parts of Chuquiraga spinosa.The ethanol extract exhibited flavonoids followed by terpenes and alkaloids.Figure 1.The morphology of the rat stomach was observed by microscopy.Moderated dysplasia was found in all groups treated with NMU.The group NMU + ChS50 showed moderated dysplasia.However, NMU + ChS250 had the lowest incidence.All the groups treated with ChS had lower incidence of inflammation and dysplasia.NMU + ChS500 showed the best protective effect on NMU induced gastric cancer.Figure 2. In regard to weights of experimental animals a significant increase of body weight was found in all groups treated with ChS.However, NMU + ChS500 had the highest value with regard to NMU group.Hematological indicators show that the triglyceride level was significantly lower in group NMU + ChS250 and group ChS500 (P < 0.05 and P < 0.01) than in group NMU.Administration of ChS at doses of 500 μg/kg significantly increased the level of HDL (P< 0.01) (Table 2).Biochemical indicators showed that the SOD, NO, MDA and CRP level was significantly lower in group NMU+ ChS250 and group NMU + ChS500 (P < 0.05 and P < 0.01) than in group NMU.Administration of Chuquiraga spinosa at the dose of 50 mg/kg significantly reduced the SOD level (P < 0.05) as compared with group NMU (Table 3).

DISCUSSION
NMU is a tumor agent which has a high affinity by H 2 than H 1 receptor , furthermore these receptors show different systems of second messengers, producing cell proliferation such as in differenced and transformed cells.NMU produces hydrolysis of inositol phosphate mediated by phospholipase C, subsequently efflux of Ca ++ and protein kinase C. 18 Dysplasia is more biologically related to malignant transformation of gastric mucosa and in this study, all animals that received the tumorinducing agents showed great dysplasia especially in the group that not received treatment. 19The age possibly influenced that dysplasia was the predominant pattern. 20In our study, the rats were 4 months (young adults) which allows their tissues respond less aggressively.The histological pattern found in the rats given ethanol extract of Chuquiraga spinosa showed discrete dysplasia with fibrotic areas. 29harmacognosy Journal, Vol 10, Issue 1, Jan-Feb, 2018 Hematological parameters showed a trend of increased HDL level and a decreased total cholesterol level in the group ChS 500 compared to the groups receiving the extract at lower doses.The lipid-lowering effect could directly influence the development of cancer because it has been evidenced in preclinical studies that statins show promising results in pancreatic, liver, colorectal and gastric cancer. 22They affect the cell division in cancer and expressed in the inhibition of proliferation, induction of apoptosis, autophagy, anti-invasion and anti-migratory effect. 23The groups that received the extract showed significantly lower values of CRP and NO.The relationship between chronic inflammation and the development of tumors was studied by Landa and showed a close relationship between the inflammatory and the increased prevalence of cancer. 24The effect of ethanol extract of Ch. spinosa in reducing CRP, MDA, SOD and NO levels could be due to the presence of flavonoids, which have the property of reducing the inflammatory mediators, and significantly reduces the odds to develop a cancerous disease. 25,26The levels of MDA and SOD were significantly lower in treated groups  compared to inductor group.MDA is related to oxidative stress and oxidative degeneration, which is closely linked to the genesis of neoplasms and dysplasias. 27sado found that reactive oxygen species (ROS) are important in the pathogenesis of cancer and cancer cells have many ROS that induces malignant phenotype. 11Landa also studied the antioxidant effect of Chuquiraga spinosa and found that its antioxidant activity is due to the flavonoid kaempferol.Chuquiraga spinosa is an anti-inflammatory and antioxidant agent and may have a protective effect for cancer. 24A significantly low level of MDA implicates potent antioxidant effect, and a significantly decreased CRP level indicates a strong anti-inflammatory effect. 28e limitations of this study include the lack of isolated metabolites present in the Chuquiraga spinosa extract.Also, its exact mechanism of it is unclear.This study includes an Animal model and add to understand human pathology but can be extrapolated to human beings in the future.
The data indicate the preventive potential in gastric cancer with a high therapeutic index.However, the complete mechanisms on which those effects are based are unknown.Further studies are needed to identify more precisely the active molecules involved in the protective effect described.

CONCLUSION
From the above results, it can be inferred that ChS at doses of 500mg/Kg attenuated NMU-induced oxidative stress because of the antioxidant activity of its bioactive metabolites.Considering the antitumorigenic, hypolipidemic, anti-inflammatory and antioxidant properties of ChS, we conclude that it has a protective effect on NMU-induced gastric cancer in rats.There is a great potential to develop Chuquiraga spinosa as an alternative therapeutic agent in gastric cancer treatment, and hence further studies are needed, particularly clinical studies, to further evaluate this effect.