@article {111, title = {GC/MS Analysis and Potential Cytotoxic Activity of Haplophyllum tuberculatum Essential Oils Against Lung and Liver Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {09/2015}, pages = {66-69}, type = {Original Article}, chapter = {66}, abstract = {

Background: Haplophyllum tuberculatum is a plant belongs to family rutacee. It is rich in volatile oils, fixed oils, alkaloids, and furanocoumarins. It is well known for the huge number of folkloric uses in middle east. \ Objective: The aim of this study is to establish the chemical composition of the essential oils of Libyan H. tuberculatum and to investigate their cytotoxic potentialities. Materials and Methods: The essential oils of the aerial parts and flowers of H. tuberculatum growing in Libya were prepared by hydrodistillation. GC/MS analyses were performed on a Shimaduz capillary gas chromatograph (GC 17A ver.3) instrument directly coupled to mass spectrometer-MS QP5050A. Oil A and F of H. tuberculatum at different concentrations (0-50 \µg/ml) in DMSO were tested for cytotoxicity against human tumor cell lines. Results: Oil yield was found 0.4 and 1.5 (v/w \%) on dry weight basis respectively. GC/MS analysis resulted in identification of total 35 compounds. 15 compounds\  were common to both oils. oil A of H. tuberculatum exhibited antitumor activities against. liver carcinoma cell line (HEPG2) and lung carcinoma cell line (H1299) 4.7 \µg/ml and 4.1 \µg/ml. Conclusion: Essential oil of the aerial parts of H. tuberculatum is potentially active against lung (H-1299), and liver (HEPG2) carcinoma cell lines. The observed cytotoxic, activities can be attributed to the dominance of \α and \γ-terpinene in this oil.

}, keywords = {Cytotoxicity, Essential oils, Haplophyllum tuberculatum, Liver cancer, Lung cancer.}, doi = {10.5530/pj.2016.1.14}, author = {Omar Mohamed Mohamed Sabry and Abeer Mohamed El Sayed and Salmin Khalid Alshalmani} }