@article {1130, title = {Extracellular-Signal Regulated Kinase Signalling Pathway Mediates the Increased Proliferation of EPCs Treated with Garlic (Allium sativum) Extract, Purple Sweet Potato (Ipomoea batatas) Extract and Vitamin C}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2017}, month = {May 2020}, pages = {442-447}, type = {Original Article}, chapter = {442}, abstract = {

The endothelial progenitor cell (EPCs) proliferation capability is reduced in the patient with stable coronary artery disease (SCAD). Garlic (Allium sativum), purple sweet potato (Ipomoea batatas), and vitamin C are proven antioxidant which potentially improve EPCs proliferation ability. Objective: To investigate the effect of garlic (Allium sativum), purple sweet potato (Ipomoea batatas), and vitamin C in EPCs proliferation from CAD patients and identify the involvement of Extracellular-Signal Regulated Kinase (ERK) Signalling Pathway. Material and Method: Mononuclear cells were isolated from SCAD patients and cultivated with colony-forming unit (CFU)-Hill medium and divided into untreated (control), garlic extract (10 mcg/ml and 100 mcg/ml), purple sweet potato extract (1 mcg/ml and 25 μg/ml), and vitamin C (10 μg/ml and 250 μg/ml). EPCs proliferation was measured using the MTT Assay. Results: This research shows that EPCs proliferation was increased in the treatment with garlic extract at 10 mcg/ml and 100 mcg/ml dose (0.267 {\textpm} 0,003 and 0.391 {\textpm} 0.008 ; p \< 0.05), purple sweet potato extract at 1 mcg/ml and 25 μg/ml dose (0.250 {\textpm} 0.005 and 0.3562 {\textpm} 0.023 ; p \< 0.001), and vitamin C at 10 μg/ml and 250 μg/ml dose (0.259 {\textpm} 0.016 and 0.306 {\textpm} 0.022 ; p \< 0.001). Increased ERK expression was found in the treatment with garlic extract, purple sweet potato extract and vitamin C. Conclusion: Garlic extract, purple sweet potato extract, and vitamin C can increase EPC proliferation through the ERK signaling pathway.

}, keywords = {Antioxidant, Endothelial Progenitor, ERK, Proliferation}, doi = {10.5530/pj.2020.12.68}, author = {Yudi Her Oktaviono and Alisia Yuana Putri and Makhyan Jibril Al-Farabi and Yesita Rizky Firmansyah and Ferry Sandra} }