@article {1132, title = {In vitro Anti-Amebic Activity of Cage Xanthones from Cratoxylum sumatranum Stem Bark Against Entamoeba histolytica}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {452-458}, type = {Original Article}, chapter = {452}, abstract = {

Background: Amoebiasis is caused by Entamoeba histolytica, which is a pathogenic species living on human colon tissues. The development of new drugs for anti-amebic are still very needed for clinical treatment. Objective: This aims to identify the compounds in Cratoxylum sumatranum for their anti-amoeba activity. Materials and Methods: In this study we used bioactivity-guided isolation and structural analysis to identified anti-amebic compounds from dichloromethane extract of Cratoxylum sumatranum stem bark. Their anti-amebic activity was determined by an in vitro cell-based assay against Entamoeba histolytica and an enzymatic assay on NAD kinase. Results: Two known compounds from the cage xanthone groups, namely cochinchinoxanthone (1) and cochinchinone D (2), were isolated. The structures of the cage xanthone compounds were established by extensive spectroscopic data analysis. Compound (1) showed the greatest level of anti-amebic activity both in cell-based and enzymatic assay, yielding IC50 values of 4.57 and 12.17 μg/mL, respectively. In contrast, compound (2) yielded IC50 values of 5.19 and 12.60 μg/mL, respectively. Conclusion: When considering the demonstrated anti-amebic activities, it becomes apparent that these compounds, isolated from Cratoxylum sumatranum stem bark, have the potential to be further developed into effective anti-amebic medicine against Entamoeba histolytica.

}, keywords = {Amoebiasis, Bioactivity-guided isolation, Cratoxylum sumatranum, Entamoeba histolytica, NAD kinase}, doi = {10.5530/pj.2020.12.70}, author = {Fendi Yoga Wardana and Defi Kartika Sari and Myrna Adianti and Adita Ayu Permanasari and Lidya Tumewu and Tomoyoshi Nozaki and Aty Widyawaruyanti and Achmad Fuad Hafid} }