@article {513, title = {GC-MS Analysis and Anti-Microbial Activities of Volatile Oil of Nardostachys Jatamansi D.C. Rhizomes Obtained from Haridwar Region, Uttrakhand}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {January 2018}, pages = {230-234}, type = {Original Article}, chapter = {230}, abstract = {

The Rhizomes of Nardostachys jatamansi D.C (Family Valerianceae) were collected from Haridwar, Uttarakhand. Aim: To identify the various volatile constituents of Nardostachys jatamansi D. C. Rhizomes and evaluation of antimicrobial activity. Method: The essential oil was collected by hydro distillation and analyzed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectroscopy (GC- MS). Volatile oil constituents, antimicrobial potential of Nardostachys jatamansi D. C. Rhizomes have been studied using different concentrations of the volatile oil against some micro-organisms. Results: A total of 10 compounds were identified from their fragmentation pattern. The Identified compounds were Calarene (20.4\%), Vardiflorene (12.3\%), \α- Panasinsen (9.7\%), \α- Santalene (4.6\%), \γ-Himachelene (17.1\%), Jatamansone (7\%), Ionol 4 (9.9\%), 2,2,7,7- Tetramethyl tricyclo[6,2,1,0 (1,6)] undec-4-ene 3-one (1.7\%), Epiglobulol (1.9\%) and Resibufogenin (8.4\%). Among them major constituents characterized was Calarene (20.4 \%) and followed by \γ-Himachelene (17.1\%) and Vardiflorene (12.3 \%). The volatile oil showed significant activity against all tested microorganisms. The maximum antibacterial activity was shown with 1\%v/v of volatile oil on Staphylococcus aureus (12.8 mm) followed by Escherichia coli (12.4 mm), whereas maximum anti-fungal activity was shown by 1\% v/v of volatile oil on Candida albicans (12.3 mm) followed by Aspergillus niger (11.9 mm). Conclusion: All identified constituents showed antibacterial activity and antifungal activity. Thus, the constituents should be further identified and analyzed by other spectrosopical techniques.

}, keywords = {Anti-microbial activity, GC GC-MS analysis, Hydro distillation, Nardostachys jatamansi DC, Volatile oil}, doi = {10.5530/pj.2018.2.40}, url = {http://fulltxt.org/article/470}, author = {Vijender Singh and Neha Rana and Mohd Ali} }