@article {540, title = {Antioxidative Properties of Blighia sapida K.D. Koenig Stem Bark Extract and Inhibitory Effects on Carbohydrate Hydrolyzing Enzymes Associated with Non-Insulin Dependent Diabetes Mellitus}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {January 2018}, pages = {376-383}, type = {Original Article}, chapter = {376}, abstract = {

Background and Aim: In Africa, the fruit, seed, leaf, and stem of Blighia sapida K.D. Koenig are used as remedy for a range of diseases. This study characterized the phytochemicals, assessed the antioxidant and inhibitory properties of the stem bark of Blighia sapida. Materials and Methods: Samples were consecutively extracted using ethanol solvent and investigated for phenol content, DPPH radical scavenging abilities, iron chelation, reducing power, \α-amylase and \α-glucosidase activities as markers of in vitro anti-diabetic activities. Phytochemicals were analyzed using gas chromatography-mass spectrometry (GC-MS) analysis. Results: The extract had radical scavenging capabilities, iron chelation and reducing power. Furthermore, the results reveal the extract had considerably high \α-glucosidase and \α- amylase inhibitory activities as the concentration increases with IC50 values of 4.17 \± 0.24 mg/mL and 5.30 \± 1.25 mg/mL, respectively. Enzyme kinetic studies indicated that the extract displayed uncompetitive inhibitor for \α-glucosidase and was a competitive inhibitor of \α-amylase. The ethanolic extract is rich in several aromatic phenolic compounds and long chain aliphatic acids. Conclusion: These results reveal the bark possessed antioxidant as well as \α -glucosidase and \α -amylase inhibitory activities.

}, keywords = {Antidiabetic, antioxidant activity, Blighia sapida K D Koenig, α-amylase, α-glucosidase}, doi = {10.5530/pj.2018.2.63}, url = {http://fulltxt.org/article/494}, author = {Oluwafemi Adeleke Ojo and Basiru Olaitan Ajiboye and Oluwatosin Debbie Imiere and Oluwatosin Adeyonu and Israel Olayide and Adewale Fadaka} }