@article {2518, title = {Antioxidant Activity and Tyrosinase Inhibition of Avocado (Persea americana Mill.) Leaves and Seeds Extracts}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {August 2025}, pages = {420-424}, type = {Original Article}, chapter = {420}, abstract = {

Background: Ultraviolet radiation (UVR) can stimulate melanin production in the skin, leading to hyperpigmentation. One way to reduce melanin formation is by inhibiting the enzyme tyrosinase. Often discarded as waste, avocado leaves and seeds are rich in flavonoids with potential tyrosinase-inhibiting properties. Objectives: This study aimed to perform phytochemical screening, measure antioxidant and tyrosinase inhibitor activities, and explore the correlation between free radical scavenging and tyrosinase inhibition in ethanol extracts of young avocado leaves (EAL(O)) and old avocado leaves (EAL(Y)), as well as the ethyl acetate extract of avocado seeds (EAS). Methods: Antioxidant activity was assessed using the 2,2-Diphennyl-1-picrylhydrazyl (DPPH) method, while tyrosinase inhibition was evaluated in vitro with mushroom tyrosinase and L-DOPA as a substrate. Results: The results of the antioxidant activity test using the DPPH method yielded IC50 values for EAL(Y), EAL(O), and EAS of 17.9{\textpm}0.15 μg/mL, 15.33{\textpm}0.02 μg/ mL, and 745.66{\textpm}6.08 μg/mL, respectively. Tyrosinase inhibition tests indicated that EAL(Y), EAL(O), and EAS could significantly inhibit tyrosinase activity (P \<0.01), with IC50 values of 10133.26{\textpm}140.12 μg/mL, 8496.20{\textpm}155.06 μg/mL, and 48375.8{\textpm}553.06 μg/mL, respectively. The correlation coefficients between tyrosinase inhibition and antioxidant activity for EAL(Y), EAL(O), and EAS were r = 0.955, r = 0.947, and r = 0.991, respectively. Conclusion: These findings suggest that EAL(Y), EAL(O), and EAS may be potential natural skin-whitening agents due to their flavonoid content and antioxidant and anti-tyrosinase properties.

}, keywords = {Persea americana Mill; tyrosinase inhibition; DPPH; L-DOPA}, doi = {10.5530/pj.2025.17.52}, author = {Shalina Sebayang and Raendi Rayendra and Ietje Wientarsih and Bambang Pontjo Priosoeryanto} } @article {2474, title = {Effects of Citrus aurantifolia Root Ethanolic Extract on Lipogenesis in Palmitate-Induced Lipid Accumulation in HepG2 Cells}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {January 2025}, pages = {77-83}, type = {Research Article}, chapter = {77}, abstract = {

Introduction: Citrus aurantifolia (lime) is mostly found in tropical and subtropical region. The lime peel and lime juice extracts have antioxidant, antidiabetic and anti-inflammatory effects. However, the pharmacological effects of the lime root remain widely unknown. Thus, the current study investigated the effects of Citrus aurantifolia root ethanolic extract (CA) on lipogenesis induced by palmitic acid (PA) in HepG2 cells. Methods: The PA-induced lipogenesis in HepG2 cells was used for measuring lipogenic gene expression and lipid accumulation of CA. Phytochemical content was also determined in CA. Results: In PA-treated group showed the state of hepatic lipid accumulation with increased lipogenic gene, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS) and sterol regulatory element binding protein1c (SREBP1c) as compared to the control group. Interestingly, administration of CA (5-10 μg/mL) effectively reduced lipid storage and significantly decreased the expression of these lipogenic gene in PA-treated cells. Notably, CA treatment increased the gene expression of fatty acid oxidation, carnitine palmitoyl transferase 1A (CPT1A) and peroxisome proliferator-activated receptor α (PPARα). Furthermore, this study found that the major bioactive component from CA was nordentatin (coumarin group). Conclusions: The results indicated that the CA treatment might be a useful agent for improving abnormal lipid metabolism in obesity-related nonalcoholic fatty liver disease.

}, keywords = {Citrus aurantifolia, Lipid metabolism, Lipogenesis, Nonalcoholic fatty liver disease, Obesity}, doi = {10.5530/pj.2025.17.10}, author = {Urarat Nanna and Jarinyaporn Naowaboot and Linda Chularojmontri and Rawiwun Kaewamatawong and Sudarat Homhual and Suvara Wattanapitayakul and Wanwisa Suwannaloet} } @article {2580, title = {Free Radical Scavenging and Anticancer Activities of Methanolic Twig Extract of Annonaceae Plant}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {December 2025}, pages = {362-365}, type = {Original Article}, chapter = {362}, abstract = {

Introduction: The Annonaceae family represents a promising source of bioactive compounds with potential therapeutic applications. This study aimed to comprehensively evaluate the antioxidant and anticancer potential of methanolic twig extracts from seven Annonaceae species. Methods: Twig specimens from Cananga latifolia, Goniothalamus elegans, Goniothalamus tamirensis, Melodorum fructicosum, Polyalthia dubia, Polyalthia cerasoides, and Uvaria fauveliana were subjected to standardized methanolic extraction. Antioxidant activity was assessed using DPPH radical scavenging assay. Anticancer potential was evaluated through Sulforhodamine B assay against three human cancer cell lines and normal human dermal fibroblasts at 25 μg/mL concentration. Results: Goniothalamus elegans demonstrated exceptional antioxidant activity (IC50 = 5.62 {\textpm} 1.21 μg/mL) comparable to ascorbic acid. In anticancer evaluation, G. elegans exhibited remarkable cytotoxicity against MCF-7 (95.19 {\textpm} 0.62\%) and HeLa (94.46 {\textpm} 1.69\%) cancer cells while demonstrating exceptional selectivity with minimal toxicity toward normal cells (19.72 {\textpm} 1.19\%). Melodorum fructicosum showed highest activity against HT-29 colon cancer cells (75.84 {\textpm} 1.57\%). Conclusions: Goniothalamus elegans demonstrated exceptional dual bioactivity with both potent antioxidant properties and selective anticancer effects. The remarkable selectivity indices and broad-spectrum anticancer activity suggest significant clinical potential. These findings provide scientific validation for traditional medicinal uses of Annonaceae species.

}, keywords = {Annonaceae, Anticancer, antioxidant activity, Cancer cell selectivity, DPPH assay, Goniothalamus elegans}, doi = {10.5530/pj.2025.17s.107}, author = {Sawitree Wongtangtintharn and Sirirath McCloskey and Rungruedee Thiwthong and U-sa Thongpairoj and Surapong Rattana} } @article {2486, title = {The Influence of Website-Based Health Information Access Media on Primary Outcomes of Metabolic Syndrome Prevention In ASN Employees}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {April 2025}, pages = {222-230}, type = {Research Article}, chapter = {222}, abstract = {

Background : Metabolic syndrome is a condition that can put all people at risk, including workers. It is important to improve health literacy related to the prevention of metabolic syndrome among workers. The association of health literacy on healthy lifestyle modification with a decrease in the prevalence of metabolic syndrome. Objective: The purpose of this study was to analyse the effect of website-based health information access media on risk factors (knowledge, self-efficacy, work stress). Methods : The type of research used is Quasy Experimental design Nonequivalent Control Group design. The sample in this study were 62 employees from 2 government offices engaged in regional finance \& assets and the implementation of the water resources network of South Sulawesi province. This study divided two groups, namely the intervention group, which received education through the {\textquoteleft}SAFETY prevent metabolic syndrome{\textquoteright} website and daily journal filling, and the control group, which received education with booklets, with both groups measured and monitored for 2 months. Results : The {\textquoteleft}SAFETY{\textquoteright} websitebased health literacy model intervention was effective in improving knowledge (p=0.000), self-efficacy (p=0.004), and reducing work stress (p=0.047) in government workers, with the significant reduction in work stress demonstrating the benefits of a technology-based approach in managing stress in the work environment. Conclusion: This study was effective in increasing knowledge and self-efficacy and reducing work stress. Future research is recommended to expand the website intervention programme with interactive modules, explore integrated self-efficacy enhancement methods, and develop specific stress management programmes with a multidisciplinary approach.

}, keywords = {Booklet, Employee, Health Literacy, Metabolic syndrome, Website}, doi = {10.5530/pj.2025.17.28}, author = {Samsiana S and Syamsiar S. Russeng and Ridwan Amiruddin and Healthy Hidayanty and Nurhaedar Jafar and Muhammad Syafar and Wahiduddin W and Chatarina Umbul Wahyuni and Anwar Mallongi} } @article {2485, title = {Literacy of Alpha-Lactalbumin (ALA) and Oleic Acid Content Improves Knowledge and Self Efficacy in Breastfeeding Milk by Mothers Who Have Babies 0-24 Months}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {April 2025}, pages = {217-221}, type = {Research Article}, chapter = {217}, abstract = {

Introduction: The world health organization (WHO) actively promotes breast milk as the best source of nutrition for babies and continues to increase the level of exclusive breastfeeding during the first 6 months to two years and reach at least 50\% by 2025. The low level of exclusive breastfeeding is likely a lack of Education about the composition of breast milk which contains peptides including α-lactalbumin (ALA) and Oleic Acid (OA) which are able to reduce the risk of breast and ovarian cancer and have therapeutic effects which have been published under the name human a-lactalbumin made lethal to tumor cells (HAMLET ), so that this literacy is able to convince mothers to give breast milk and is a health threat to mothers that it is important to give breast milk because it has anti-cancer substances that can prevent cancer. Method: This type of research is quantitative, with a Quasi Experimental Nonequivalent Control Group Design. In this research , the experimental group was treated by providing education and assessing and using knowledge and self-efficacy instruments . Mean while, the control group received education through the KIA book. The target population in this study is mothers who have babies aged 0-24 months in the working area of the Ujoh Bilang Health Center, Mahakam Ulu Regency. The sampling technique is purposive sampling. Results: there is a difference in knowledge literacy, Self Eficaccy on Breast Milk Feeding (ASI) by mothers who have babies 0-24 months in the intervention and control groups before and after being given breast milk literacy with a p value of 0.000 (p \< 0.005) which indicates that the hypothesis is accepted, namely there is an effect of breast milk literacy on knowledge and self-efficacy. Conclusion: literacy of alpha-lactalbumin (ALA) and oleic acid (OA) content increases knowledge and selfefficacy in providing breast milk by mothers who have babies 0-24 months.

}, keywords = {alpha-lactalbumin, Breast milk literacy, knowledge, oleic acid and breast milk, self-efficacy}, doi = {10.5530/pj.2025.17.27}, author = {Nursari Abdul Syukur and Citrakesumasari and Anwar Mallongi and Syamsiar S. Russeng and Masni Mappajanci and Aminuddin Syam and Syamsuar S and Endah Wahyutri} } @article {2509, title = {Metabolomic Study on Indonesian Green Tea (Camellia Sinensis L.) Cultivation}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {June 2025}, pages = {331-341}, type = {Research Article}, chapter = {331}, abstract = {

Background: Indonesia has been one of the largest tea producers in the world with one of the reputable plantations in Ngargoyoso region, Java Island in which the plantation specified in five green teas variants, TRI 2024, TRI 2025, Gambong, Kiara and Chinruan. Despite their premium quality, there were limited metabolites information related to varieties, altitude, and plucking position existed in Ngargoyoso. Objective: this research aimed to investigate metabolite profiling affected by varieties, plucking positions and altitudes in the tea plantation grown in Ngargoyoso, Indonesia. Material and Methods: Green tea (Camellia sinensis L.) grown in the plantation are five varieties TRI 2025, Gambong, TRI2024, Kiara and Chinruan in Ngargoyoso. Results: 1H NMR metabolomics protocol successfully identified TRI 2025 variety to have higher levels of theanine, catechins and caffeine than other varieties. The young leaves plucking position showed a higher level of catechin, EGCG, caffeine, theobromine, and L-glutamine than the older ones. While green tea at 1159 masl contains a higher level of theanine, L-glutamin, α-glucose, β-glucose, quinic acid, and succinic acid than those at 899 masl. This makes the TRI 2025 variety at the young position can be recommended to be the best quality in taste and benefits. Conclusion: Present study might suggest the tea quality based on metabolites profiling both for taste and benefits.

}, keywords = {Tea leaves; Camellia sinensis; metabolite profiling; 1H-NMR; Indonesia}, doi = {10.5530/pj.2025.17.43}, author = {Dinar S. C. Wahyuni and Raissa. K. Putri and Anik Arniyanti and Sasha H. Audita and Syaiful Choiri and Saptono Hadi and Rita Rakhmawati and Estu R. Nugraheni and Soerya D. Marliyana and Yudi Rinanto and Ari Satia Nugraha and Ahmad Ainurofiq} } @article {2464, title = {Preconception Posyandu Performance Assessment Instrument with Europan Foundation Quality Management (EFQM) Method: Indicators, Validity and Reliability}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {January 2025}, pages = {40-46}, type = {Original Article}, chapter = {40}, abstract = {

Preconception Posyandu is one of the preconception women{\textquoteright}s health services created to help reduce MMR, IMR and stunting prevalence in Banggai District. This study aims to create a performance assessment instrument for the Preconception Posyandu so that it can maintain the existence of the service. The development of performance assessment indicators through Focus Discussion Group (FGD) activities using the Europan Foundation Quality Management (EFQM) method consists of using 9 variables. We involved 30 village midwives and 30 preconception women at three health centers within Luwuk City to test the performance assessment instrument. Internal reliability was assessed with Cronbach{\textquoteright}s alpha and criterion validity. The performance appraisal instrument using the EFQM method obtained development results, namely from 52 indicator questions made into 92 questions, then after testing the validity of the instrument, 81 questions were declared valid and 11 questions were invalid. The results of Cronbach{\textquoteright}s alpha value, there are 5 variables with strong reliability (alpha\> 0.80), namely leadership variables, strategic policies, staff management and partnerships and resources, while there is 1 variable with perfect reliability (alpha\> 0.90), namely process variables, and 3 variables with sufficient reliability (alpha \> 0.70), namely performance outcome variables, customer satisfaction and impact on society. The questions generated from the development of indicators can be used to measure performance. The service of the Preconception Posyandu Program that has been running so far, this is evidenced by the average value of the reliability test which is 0.83, which means strong reliability.

}, keywords = {EFQM, Instrument, Performance Appraisal, Preconception Health Post}, doi = {10.5530/pj.2025.17.5}, author = {Fitrianty Sutadi Lanyumba and Muhammad Syafar and Darmawansyah D and Apik Indarty Moedjiono and Healthy Hidayanty and Wahiduddin W and Faizal Fahmi and Anang S Otoluwa and Anwar Mallongi} } @article {2567, title = {A Study on Arsenic and Arsenic-Containing Mineral Medicines Referenced in Ancient Books of Traditional Mongolian Medicine}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {December 2025}, pages = {270-274}, type = {Original Article}, chapter = {270}, abstract = {

Introduction: Arsenic was used to distinguish between red stone poison and white stone poison in traditional medicine from ancient times. However, there is no specific study conducted on "stone arsenic" based on ancient Mongolian medical books and manuscripts. Therefore, this study aims to collect and analyse information on arsenic recorded in ancient traditional medical scriptures and books written in Mongolian and Tibetan as early as the 19th to late 20th centuries, with a focus on its medical applications and associated terminology. Methods: The hermeneutic research method emphasises the interpretive analysis of ancient texts, communications, and their interrelations. Employing the hermeneutics method, we focused on interpreting the meanings within ancient medical texts and manuscripts, including the terminology of the sources selected for analysis. The checklist method was used to categorise and list the facts and information about arsenic in the study sources. The relevant components of the collected information and facts regarding arsenic and arsenic-containing mineral medicines were examined. Results: Several sources of traditional Mongolian medicine have mentioned the use of arsenic as a standalone substance. Additionally, there is limited information about its use in combination with other medications. During the research, it was discovered that an ancient manuscript mentioned its use as an active ingredient. This manuscript, a single-page scripture, is poorly written but contains rare information. The active ingredient of this medicine is arsenic, and the other four ingredients are sulphur native, sal ammoniac, realgar, and resin of the plant Shorea robusta. It is mixed with aged oil and applied to various wounds, and is regarded as one of the most effective remedies for all ulcers and wounds. Conclusion: The research results revealed that there are several clear references to arsenic in ancient traditional Mongolian medical books and manuscripts written in Tibetan and Mongolian. The Tibetan name for arsenic is rdo dug, the Mongolian name is kh{\"u}ntsel, and the Latin name is Arsenicum. The findings indicate that arsenic is incorporated into several traditional prescriptions and serves as an active component in certain medicinal formulations.

}, keywords = {Arsenic, ingredient, manuscript, mineral medicines, Traditional medicine}, doi = {10.5530/pj.2025.17.96}, author = {Sainjargal Ayush and Selenge Erdenechimeg and Ao Wuliji and Hua Naranmandura and Bold Sharav and Javkhlan Bold} } @article {2412, title = {Abnormalities in Hemostatic Parameters Related to Hemodialysis in End-stage Kidney Pathology: A Narrative Review}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {October 2024}, pages = {1223-1230}, type = {Review Article}, chapter = {1223}, abstract = {

Chronic kidney disease (CKD) is a progressively prevalent global health issue. During the initial phases of the condition, CKD is commonly linked to a tendency for excessive blood clotting. While in the end-stage of disease, patients undergoing hemodialysis have a multitude of hemostatic abnormalities. These include prolonged bleeding time, altered platelet count, prolonged PT and aPTT, elevated FDPs and D-dimer, dysregulated vWF activity, and abnormal thrombin generation. These changes result from a combination of uremic toxicity, endothelial dysfunction, inflammatory states, and the effects of the hemodialysis procedure itself. Understanding these mechanisms is crucial for managing and mitigating the bleeding and thrombotic risks in this patient population. This review aims to systematically investigate the effects of hemodialysis on key hemostasis parameters in CKD patients.

}, keywords = {Coagulation, End-stage Kidney Disease (ESKD), Hemodialysis, Hemostasis}, doi = {10.5530/pj.2024.16.200}, author = {Bassam A. Hazae and Yetti Hernaningsih and Puspa Wardhani and Fatima Albadwi and Nastasya Nunki} } @article {2258, title = {Analysis of the Giving of Mp-Asi Through Food Based on Majene{\textquoteright}s Local Wisdom for Stunting Prevention}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {372-377}, type = {Research Article}, chapter = {372}, abstract = {

Background: Majene Society own tradition and knowledge typical in selecting, processing, and consuming food. Use food local can maintain diversity food and culture local. Food local has proven effective for growth baby optimally. Goals: With do comprehensive analysis to provision of MP-ASI through foodbased wisdom local Majene, expected can an effective and sustainable stunting prevention program was developed. Method: Method research used is study qualitative with design phenomenology. Informant in study This are 103 Baduta mothers who are exclusively breastfed, and live in rural districts Pamboang and District Tameroddo. Data analyzed use content analysis through N-Vivo 12 app. Results: Food tree chief in Tameroddo is rice red Because rice red become plant lots of fields found and easy grows in mountainous areas. Meanwhile in the Coastal Region Pamboang Still consistent with rice white or yams. Typical vegetables is leaf moringa, Langurru {\textquoteright}, and Lallere {\textquoteright}. Government Regency Majene push planting leaf moringa in each home easy get and help in increase continuity food, savings cost, and independence food in Majene. Besides That, leaf moringa of course known known as " tree magic " because mark high nutrition. Majene Society own access and consumption vegetables langngurru {\textquoteright} and lallere {\textquoteright} are just that found in the area mountains Tameroddo. Usually leaf This used as plant medicine, but in this area made as processed vegetables with add mango sour. Side dish typical Majene known with called " Baupapi ". Habit consumption child given Eat with Fish sauce is considered Already enough , even often this fish sauce considered as replacement vegetables , though very easy found various the main fish species in the coastal area Pamboang . Known Pineapple in local language Majene as {\textquotedblleft}Pandeng{\textquotedblright} is easiest fruit found Because grow with flourish in the yards House citizen. Findings food local the has content test nutrition and recipe test so that can used as the proper MP-ASI menu for given to baby for optimal growth. Conclusions and Suggestions: Important For ensure that kids get too consumption of fish or other protein sources as well as nutrition from vegetables and sources food other. Order child get appropriate nutrition through provision of adequate MP-ASI. Recommended to nanny , power health and government regency Majene to encourage provision of local MP-ASI with still notice mark appropriate nutrition , MP -ASI module as research output This can used as guidelines Proper provision of MP-ASI .

}, keywords = {Food based Local, MP-ASI, Stunting Prevention}, doi = {10.5530/pj.2024.16.57}, author = {Evawati and Nurhaedar Jafar and Healthy Hidayanty and Sudirman Natsir and Razak Thaha and Ridwan Amiruddin and Citrakesumasari and Lucy Widiasari and Anwar Mallongi} } @article {2308, title = {Analysis of the Inpatient Medical Records Management System at Sandi Karsa Hospital Makassar}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {June 2024}, pages = {650-655}, type = {Research Article}, chapter = {650}, abstract = {

Objectives: Management of medical records at Sandi Karsa Hospital Makassar is still lacking in the process of assembling, reporting, and filing. This study aims to analyze the inpatient medical record management system at Sandi Karsa Hospital, Makassar. Methods: The type of research used in this research is qualitative research and data collection using interviews, observation, and documentation with key informants and supporting informants using purposive sampling techniques. Result: There is a shortage of medical records officers, and they have not received training. There are fewer computers available, SIMRS is only integrated with patient registration, and the management room is combined with the filing room. The assembly process has not been carried out in the last few months, reporting has been delayed, and there is a backlog of files. Implementation of management SOPs has not been optimal, and socialization activities have not been carried out. Completeness and returns are not up to standard. Medical record quality indicators are low, and there are no feedback activities. Conclusion: Lack of staff they have never participated in training and socialization regarding SOPs. There is a lack of facilities such as computers, the medical record room has lots of files, SIMRS is not yet integrated with medical record management, and there are delays in returns and incomplete filling out of medical record files. The hospital can provide outreach and hold regularly scheduled meetings to evaluate or assess officers{\textquoteright} performance managing medical records.

}, keywords = {Hospital, Inpatient, Management, Medical Records, System}, doi = {10.5530/pj.2024.16.102}, author = {Try Ganjar Wati and Amran Razak and Darmawansyah and Muhammad Yusran Amir} } @article {2337, title = {Antigout Activity of Terap (Artocarpus odoratissimus Blanco) Leaves Extract: Leaves Characteristic and In Vitro Studies}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {764-769}, type = {Original Article}, chapter = {764}, abstract = {

Terap leaves (Artocarpus odoratissimus Blanco) contain various chemical constituents, making it a herbal medicine prospective source, particularly as an antigout. This research aim to determinate characteristics of terap leaves and in vitro antigout activity of terap leaves ethanolic extract. The determination leaves characteristics included macroscopic and microscopic analysis, water and ethanol soluble extract content, and drying shrinkage. The extract antigout activity was determinated through inhibition of xanthine oxidase enzyme in vitro. Leaves characteristics analysis revealed that mature leaves are approximately 31.5 cm long and 18 cm wide. Terap leaves are single leaves, elliptical, blunt leaf tips, rounded bases, rough surfaces, undulating edges, pinnate veins, and yellow veins. Microscopic analysis reveals the presence of anomocytic-type stomata, trichomes, glandular hairs, resin ducts, and trachea. Terap leaves have a water-soluble extract content of 8.72\%, ethanol-soluble extract content of 4.29\%, and drying shrinkage of 11.38\%. The antigout activity tests results indicates that ethanol extract inhibits xanthine oxidase enzyme with an IC50 of 89.63 μg/mL. Terap leaves have antigout properties, so they have the potential to be developed into a quality antigout herbal medicine.

}, keywords = {Antigout Activity, Artocarpus odoratissimus, Macroscopic and Microscopic Analysis., Xanthine Oxidase Enzyme}, doi = {10.5530/pj.2024.16.127}, author = {Nisa Naspiah and Retno Widyowati and Sukardiman} } @article {2343, title = {Association of CRP, Procalcitonin, Lactate, and Albumin Levels with In-Hospital Mortality Post-Definitive Laparotomy in Patients with Complicated Intra-Abdominal Infections}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {805-808}, type = {Original Article}, chapter = {805}, abstract = {

Introduction: Complicated intra-abdominal infection (cIAI) still has a high mortality rate due to organ dysfunction despite advances in supportive care. Unlike other sources of septicemia, cIAI requires source control surgery, which is crucial for improving outcomes. The strategy for source control varies depending on the degree of inflammation associated with cIAI; the more severe the inflammation, the less aggressive the surgery needs to be. Therefore, we need a reliable parameter to predict the degree of inflammation before any physiological rearrangement or organ dysfunction occurs due to excessive inflammation from surgery. The literature shows that CRP, procalcitonin, lactate, and albumin are associated with the degree of inflammation. Thus, it{\textquoteright}s necessary to study about mentioned parameters for being reference determination strategy of surgery in cIAI, classical definitive laparotomy, or rapid source control laparotomy (RSCL). Methods: We have collected data from CIAI patients at Dr. Soetomo Regional General Hospital in Surabaya, Indonesia, covering November 2022 to April 2024. Our primary focus is assessing the inflammation level associated with the compensated or decompensated phase. The decompensated phase signifies an excessive inflammatory response, with one indication being in-hospital mortality. Subsequently, we performed univariate and multivariate analyses using the SPSS program to determine which laboratory parameters (CRP, Procalcitonin, Lactate, and Albumin) are most associated with in-hospital mortality. Results: Between November 2022 and April 2024, there were 309 patients with complicated intra-abdominal infections (cIAI). Among the patients, 61.8\% were male and 38.2\% were female. The majority of patients (27.17\%) were aged 61-70. The causes of cIAI included perforated appendicitis (22.65\%), perforated peptic ulcer (21.69\%), complications from previous surgeries (18.13\%), large bowel perforation (12.29\%), small bowel perforation (11.33\%), intraperitoneal abscess (11.33\%), and other causes (2.58\%). Statistically, lactate was found to be the most accurate predictor of intraoperative hemodynamic instability (p-value \< 0.001; correlation coefficient of 0.481), followed by albumin (p-value \< 0.001; correlation coefficient of 0.357). CRP and Procalcitonin were less accurate, with correlation coefficients of 0.182 and 0.272, respectively. The determined cut-off points for lactate and albumin were 1.94 and 2.73, for CRP and Procalcitonin were 23,24 and 47,95. Abnormal laboratory finding in our study mean CRP above cut off point, Procalcitonin above cut off point, lactate above cut off point and albumin below cut off point. More than 2 laboratories finding, in-hospital mortality 66,7\%, 2 laboratory finding in-hospital mortality 42,8\% and only 1 laboratory finding in-hospital mortality 28,4\%. Conclusions: CRP, Procalcitonin, Lactate and Albumin have relation statistically significant with in-hospital mortality. Lactate and albumin are better than CRP and procalcitonin in our study, and consider RSCL for more than 2 parameter abnormal laboratory findings CRP, procalcitonin, lactate or albumin.

}, keywords = {Complicated intraabdominal infection, Damage control laparotomy, Inflammatory mediators}, doi = {10.5530/pj.2024.16.133}, author = {Mochamad Syahroni Fardiansyah and Tomy Lesmana and Edwin Danardono and Denny Septarendra and Ismu Nugroho and Adhitya Angga Wardhana and Anton Sugianto and Rena Normasari} } @article {2358, title = {Combination Bitter, Ginger, Turmeric Extract in Mice: Acute and Sub Acute Toxicity Analysis}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {916-922}, type = {Research Article}, chapter = {916}, abstract = {

Herbal plants are widely used as traditional medicine for generations. Some of them are bitter, in Indonesian called Sambiloto (Andrographis paniculata), red ginger in Indonesian called Jahe merah (Zingiber officinale var rubrum), and turmeric in Indonesian called Kunyit (Curcuma domestica). This study tested extracts containing a combination of three ingredients, hereinafter named {\textquoteright}SIJAKUN{\textquoteright} extract (SambIloto, JAhe, KUNyit){\textquotedblright}. This combination has good benefits for the body, especially as an anti-inflammatory and antioxidant. This study aims to measure the acute and subacute toxic effects of {\textquoteright}SIJAKUN{\textquoteright} extract. This is a true experimental study. In the acute toxicity study, the SIJAKUN extract was given to 5 groups of mice a doses of 1000 mg/kg, 2,000 mg/kg, 3,000 mg/kg, 4,000 mg/kg, and 5,000 mg/kg. After being observed for 24 hours, the number of dead mice from each group was counted to calculate the LD50 of the {\textquoteright}SIJAKUN{\textquoteright} extract. Whereas in subacute toxicity, 5 groups of mice were given 0.5 ml of CMCNa solution, {\textquoteright}SIJAKUN{\textquoteright} extract at doses of 25 mg/kg, 75 mg/kg, 150 mg/kg, and 25 mg/kg ibuprofen, the test substance was given orally once daily for 28 days. Then an examination was carried out: SGOT, SGPT, BUN, and serum creatinine. the results of subsequent examinations were analyzed statistically. To assess the safety of the SIJAKUN combination, it is necessary to conduct research to determine the acute and sub acut toxicity of SIJAKUN to the liver and kidneys. Based on the results of acut toxixity study conducted, no experimental animals died at one dose of 1000, 2000, 3000, 4000 and 5000 mg/kgbb {\textquoteleft}SIJAKUN{\textquoteright} extract. Based on these results, the LD50 of {\textquoteright}SIJAKUN{\textquoteright} extract was above 5000 mg/kgbb. Therefore, it can be said that {\textquoteright}SIJAKUN{\textquoteright} extract is a non-toxic compound. In the subacute toxicity study, examination result of SGOT, SGPT, BUN, and serum creatinin was not change significantly, with P value \> 0.05. This result indicating no significant difference between the control group and the treatment group. It can be said that the sub-acute toxicity test found that {\textquoteright}SIJAKUN{\textquoteright} extract did not toxic for liver and kidney. In acute and subacute toxicity studies that has been carried out, {\textquoteright}SIJAKUN{\textquoteright} extract was classified as a non-toxic compound, so it is safe to use as herbal medicine.

}, keywords = {Acute and Sub Acute Toxicity, Ginger, LD50., SIJAKUN (bitter, turmeric)}, doi = {10.5530/pj.2024.16.148}, author = {Handayani H. and Renny Novi P. and Andik Ferdiantoro and Afira Febriani S. W. and Rifky Dwi Aditya Iryawan and Diaz Syafrie A. and Rahadian Zainul and Arif Nur Muhammad Ansori and Mochammad Aqilah Herdiansyah} } @article {2333, title = {Comparative Analysis Molecular Simulation IL6R Alpha with TCZ and HIL6: Mechanism in Inflammatory Responses}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {738-743}, type = {Original Article}, chapter = {738}, abstract = {

Introduction: In cases of inflammation, there is typically a connection between IL6R and HIL6. If there is an excessive level of activity in this connection, it can lead to a cytokine storm. Tocilizumab (TCZ), also known as AntiIL-6R, is a biologic drug that is a recombinant humanized monoclonal antibody. It is specifically used to treat inflammatory and autoimmune diseases that are associated with cytokine storms. Method: This study utilizes in silico analysis to assess the ability of TCZ, a biosimilar, to block IL6R and compares it to the blocking effect of HIL6. Validation of the 3D structure of the IL6R was performed using a Ramachandran plot. Results: The IL6R alpha subunit had a validation score of 97.86\%, while the IL6R beta subunit had a validation value of 95.54\%. The molecular docking analysis reveals that the TCZ light chain forms a complex with IL6R, yielding a docking score of -16.4 kcal mol-1. Similarly, the TCZ heavy chain also interacts with IL6R, resulting in a docking value of -15.5 kcal mol-1. Notably, both scores are higher than the docking score of the control, which involves IL6R with HIL6, measuring -12.5 kcal mol- 1. The root mean square fluctuation (RMSF) value of the IL6R protein in the presence of TCZ (Tocilizumab) is consistently below 2, with an average range of 0.04-0.09. Conclusion: The affinity between IL6R and TCZ is greater than the affinity between IL6R and HIL6.

}, keywords = {Binding affinity, HIL6, IL6, IL6R, Molecular docking, Molecular dynamics.}, doi = {10.5530/pj.2024.16.123}, author = {Engla Merizka and Septelia Inawati Wanandi and Budiman Bela and Silvia Tri Widyaningtyas and Fadilah Fadilah} } @article {2224, title = {Concordance of Sputum and Feces Samples for Detecting Mycobacterium Tuberculosis using Xpert{\textregistered} MTB/RIF Ultra}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {February 2024}, pages = {167-173}, type = {Research Article}, chapter = {167}, abstract = {

Introduction: Tuberculosis (TB) remains a disease with high morbidity and mortality worldwide, and Indonesia ranks among the countries with the highest TB prevalence. There is a need to develop improved detection tools and explore alternative sample sources beyond sputum. Feces samples are one such nonsputum alternative. Xpert{\textregistered} MTB/RIF Ultra is a novel diagnostic tool used in Indonesia. This study aims to assess the concordance of both sputum and feces samples in detecting Mycobacterium tuberculosis (MTB) using Xpert{\textregistered} MTB/RIF Ultra. Methods: An analytical observational study with a cross-sectional design was conducted on TB subjects at Dr. Soetomo Regional Public Hospital (RSUD Dr. Soetomo), Surabaya, and several community health centers (puskesmas) in Surabaya. Sputum and feces samples were collected from the same subjects. These subjects underwent Feces acid-fast bacilli (AFB) tests, sputum and Feces Xpert{\textregistered} MTB/RIF Ultra tests, and sputum culture tests (considered the gold standard), as well as rifampicin resistance tests for positive cultures. Sensitivity and positive predictive value (PPV) tests were conducted using Medcalc software, and the concordance test employed the Kappa value. Results: The study involved 71 research subjects. The sensitivity of Feces AFB tests, sputum, and Feces Xpert{\textregistered} MTB/RIF Ultra tests was 7.3\%, 97.6\%, and 97.6\%, respectively. The Cohen{\textquoteright}s Kappa consistency test for Feces AFB tests and sputum culture produced a Kappa value of 0.063 (p \> 0.05). The Cohen{\textquoteright}s Kappa consistency test on sputum and Feces Xpert{\textregistered} MTB/RIF Ultra tests yielded a Kappa value of 0.409 (p \< 0.05). The Cohen{\textquoteright}s Kappa consistency test on sputum and Feces Xpert{\textregistered} MTB/RIF Ultra tests compared with the rifampicin resistance tests resulted in Kappa values of 0.902 and 0.951 (p \< 0.05). The CT value of Feces Xpert{\textregistered} MTB/RIF Ultra tests was higher than that of sputum Xpert{\textregistered} MTB/RIF Ultra tests. Conclusion: A concordance exists between the results of sputum and Feces Xpert{\textregistered} MTB/RIF Ultra tests, but no concordance is observed between the results of Feces AFB tests and sputum culture tests. The higher CT value of Feces Xpert{\textregistered} MTB/RIF Ultra tests compared to sputum Xpert{\textregistered} MTB/RIF Ultra tests indicates a lower bacterial load in feces. Feces can be considered a viable alternative sample to sputum for MTB detection using Xpert{\textregistered} MTB/RIF Ultra.

}, keywords = {Feces AFB test, Feces Xpert{\textregistered} MTB/RIF Ultra test, Mycobacterium tuberculosis (MTB), rifampicin resistance test, Xpert{\textregistered} MTB/RIF Ultra}, doi = {10.5530/pj.2024.16.23}, author = {Dewi Rochmawati and Puspa Wardhani and Yessy Puspitasari and Tutik Kusmiati and Atika and Hartono Kahar} } @article {2444, title = {Cytotoxic Potential of Mitragyna speciosa as Anticancer - A Review}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {December 2024}, pages = {1418-1423}, type = {Review Article}, chapter = {1418}, abstract = {

Background: Herbal treatment has been proposed and researched as an alternative to cancer treatment. One of the reasons contains compounds that have cytotoxic effects. Mitragyna speciosa are known to contain alkaloids and have a cytotoxic effect. Objective: This review aimed to provide information about preclinical studies and investigates the cytotoxicity or anticancer activity of M. speciosa. Methods: Search articles through PubMed, Springer, and Science Direct databases focusing on preclinical trials according to PRISMA guidelines. A database search yielded a total of 206 identifiable studies. Then duplicate removal and feasibility screening were carried out, resulting in 11 studies that were eligible for final analysis. Results: The anticancer potentials reviewed in this study include Neuroblastoma, Leukemia, Colon Cancer, Breast Cancer, Kidney \& Liver Cytotoxicity, Glutathione Transferases Metabolizing Enzymes, Alkaloid Combination of M. speciosa \& Cisplatin, Alkaloid Combination of M. speciosa \& Doxorubicin and Mutagenic-Antimutagenic Activity of M. speciosa. Extracts and dominant alkaloids of M. speciosa have the potential for anticancer neuroblastoma, leukemia, colon, lung and breast cancer. Based on the safety aspect of the mitragynine compound, there is no mutagenic effect on cells. Conclusion: M. speciosa contains the dominant active alkaloid compound, mitragynine. Extracts and alkaloids dominant in M. speciosa have the potential as an anticancer.

}, keywords = {Alkaloids, Cancer, Cytotoxicity, M. speciosa, Mitragynine}, doi = {10.5530/pj.2024.16.228}, author = {Puja Adi Priatna and Retno Widyowati and Sukardiman} } @article {2347, title = {Drug Management and Service Quality in Outpatient Pharmacies at the Haji Padjonga Daeng Ngalle Regional General Public Hospital in Takalar Regency, Indonesia}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {838-844}, type = {Research Article}, chapter = {838}, abstract = {

Background: The provision of comprehensive individual health care services in outpatient units is intricately linked to drug management practices and the quality of available pharmacy services, ensuring optimal medication supply and use. Objective: This study aimed to assess the practices of drug management and the quality of outpatient pharmacy services at Haji Padjonga Daeng Ngalle (HPDN) Regional General Public Hospital in Takalar Regency, Indonesia. Method: The evaluation of drug management involves conducting in-depth interviews in the outpatient unit, focusing on planning, budgeting, procurement, storage, and distribution, compared with government regulatory standards. The quality of pharmacy services was assessed using the Solvin technique with 100 respondents, evaluating five dimensions: physical evidence, reliability, responsiveness, assurance, and empathy, using the servqual questionnaire with a Likert scale (ranging from very dissatisfied = 1, dissatisfied = 2, somewhat satisfied = 3, satisfied = 4, to very satisfied = 5). Results: Research findings indicated the level of compliance of drug management with regulations as follows: planning (66.67\%), budgeting (60\%), procurement (100\%), storage (100\%), and distribution (100\%). The quality of pharmacy services demonstrated high questionnaire validity (r >= 0.714), and the average dimension was a score of 4, indicating satisfactory satisfaction levels. Conclusion: This study reflects HPDN hospital{\textquoteright}s compliance with regulations while identifying specific areas for improvement to enhance the quality of pharmacy services.

}, keywords = {Drug management, Hospital, outpatient unit., patient service quality}, doi = {10.5530/pj.2024.16.137}, author = {Yunita Cahyani Pratiwi and Darmawansyah and Sukri Palutturi and Amran Razak and Atjo Wahyu and Anwar Daud and Anwar Mallongi} } @article {2424, title = {The Effect Ethanol Extract of Phyllanthus niruri l on Malondialdehyde (MDA) Expression and Extracellular Signal- Regulated Protein Kinase-1 (ERK-1) on Vaginal Epithelial Cell Thickness in Menopausal Mice}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {December 2024}, pages = {1305-1310}, type = {Original Article}, chapter = {1305}, abstract = {

Introduction: Menopause is the condition of the ovaries stops produce estrogen so that resulting in vaginal bleeding atrophy that is become dry cause pain moment relate sexual so that lower quality life. The ROS pathway with MAPK regulates proliferation, differentiation, motility, and survival cell life. Research purposes is know influence giving extract Phyllanthus niruri l against Malondialdehyde (MDA) expression and Extracellular Signal-Regulated Protein Kinase-1 (ERK-1) expression against thickness cell vaginal epithelium of menopausal model mice. Material from Phillantus niruri l processed become extract. Treatment animal try mice (mus muscullus) first acclimatized during one next week done ovariectomy of both ovaries, after two weeks checked vaginal examination to be sure phase diestrus (menopause). Stage treatment given extract for 21 days with dose different 14 mg, 28 mg and 56 mg/20gBW/ day. Methods: True Experimental research method with Post Test only with control group design. Data analysis used one way ANOVA. Results: The research group that produced the highest average expression of Malondialdehyde (MDA) was the control group. The highest expression of Extracellular Signal-Regulated Protein Kinase-1 (ERK-1) was in the P3 treatment group (dose 56 mg/20gBW/day). The results of statistical analysis showed that there was a significant effect of Phyllanthus niruri l extract on decreasing MDA expression with a sig value of 0.000 \< p = 21 0.001 and increasing ERK-1 with a sig value of 0.000 \< 0.001, but there was no effect on increasing the thickness of the vaginal wall epithelial cells in menopausal model mice. with a sig value of 0.220 \> 0.05. Conclusion: The three doses of phillantus niruri decreased MDA and increased ERK-1. The Folin-Ciocalteau.

}, keywords = {ERK-1, MDA, Menopausal Mice, Phyllanthus niruri l, Vaginal epithelium}, doi = {10.5530/pj.2024.16.210}, author = {Lailatul Fadliyah and Hendy Hendarto and Lestari Sudaryanti and Imam Susilo and Anwar Ma{\textquoteright}ruf and Emuliana Sulpat and Endah Sri Wijayanti and Maya Septriana} } @article {2429, title = {Effect of Cacao Bean Extract as a Paracetamol Adjuvant on Pain Scale and Tumor Necrosis Factor-Alpha in Neuropathic Pain: An Animal Model Study}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {December 2024}, pages = {1336-1341}, type = {Research Article}, chapter = {1336}, abstract = {

Introduction: One treatment for neuropathic pain is paracetamol. Meanwhile, cacao bean extract is a traditional remedy developed for pain management. Objective: Analyzing effect of combining cacao bean extract and paracetamol on pain scale and tumor necrosis factor-alpha (TNF-α) in neuropathic pain. Methods: Subjects were randomized post-test only control group design from 28 mice (Mus musculus) to 4 groups: G0 (control), G1 (paracetamol only), G2 (cacao + paracetamol), and G3 (cacao + {\textonehalf} doses paracetamol). The subject assessed pain scale using von Frey test and TNF-α. The statistical analysis includes paired t-tests, Wilcoxon, one-way ANOVA, Kruskal Wallis, and Pearson correlation tests with p \<0.05. Results: The combination of cacao bean extract and paracetamol resulted in a pain scale of 2.57 {\textpm} 1.10 gf, with significant differences observed among the four groups (p \<0.001). Significant differences in pain scale scores were found in four groups (p \<0.001), including G0 (p = 0.006), G1 (p \<0.001), G2 (p \<0.001), and G3 (p \<0.001). After treatment, the average TNF-α levels was 86.96 {\textpm} 23.73 ng/mL, with significant differences observed among the four groups (p \<0.001). There was a strong correlation between the pain scale and TNF-α levels (p \<0.001). Conclusion: In an animal model of neuropathic pain, using cacao bean extract as a paracetamol adjuvant significantly reduces pain scale (as measured by the von Frey test) and TNF-α levels.

}, keywords = {Cacao, Neuropathic pain, pain scale, Paracetamol, TNF-α}, doi = {10.5530/pj.2024.16.215}, author = {Faiz Muhammad Ammar and Christrijogo Sumartono Waloejo and Herdiani Sulistyo Putri and Kohar Hari Santoso and Prananda Surya Airlangga and Budi Utomo} } @article {2388, title = {The Effect of HBOT on SIRT-1 and SYNDECAN-1 as Therapeutic Targets for Endothelial Dysfunction}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {October 2024}, pages = {1090-1095}, type = {Research Article}, chapter = {1090}, abstract = {

Background: The effect of HBO2 alone on Sirt-1 and Syndecan-1 is unknown, even though both molecules are involved in preventing endothelial dysfunction. This study aims to determine the effect of HBO2 on Sirt-1 and Syndecan-1 as therapeutic targets for endothelial dysfunction. Method: This study employed a true experimental post-test design. Twenty male Sprague Dawley rats aged 12-14 weeks were divided into two groups. Diving was carried out in an animal hyperbaric chamber with a dose of 2.4 ATA for 60 minutes. All data were collected 18 hours after diving. Results: Our study revealed that the administration of HBO caused an increase in serum MDA and endothelial NF-kB levels (p = 0.007; p = 0.001, respectively) without an increase in any inflammatory markers, specifically IL-1 and VCAM-1 levels (p = 0.707; p = 0.168, respectively). HBO2 decreased Syndecan-1, a marker of endothelial injury (p = 0.026), but did not affect endothelial eNOS and Sirt-1. Conclusion: HBO2 did not cause endothelial injury and inflammation, but the dose used was not enough to increase Sirt-1 levels. Additional research is needed to determine a hormesis dose that can increase Sirt-1 levels.

}, keywords = {Endothelial dysfunction, HBO2, Sirt-1, Syndecan-1}, doi = {10.5530/pj.2024.16.177}, author = {Sofia Wardhani and Aryati Aryati and Bambang Purwanto} } @article {2226, title = {Effect of Lactiplantibacillus plantarum IS-10506 on Accelerating Repair of Ketorolac-Induced Gastric Ulcers in Wistar Rats}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {February 2024}, pages = {181-185}, type = {Original Article}, chapter = {181}, abstract = {

A gastric ulcer arises due to an imbalance between the stomach{\textquoteright}s aggressive and defensive factors, one of which can be induced by ketorolac. The gastric mucosa serves as a protective layer against gastric damage. Probiotics may enhance mucosal secretion, reinforcing their barrier function. This study evaluated the effect of Lactiplantibacillus plantarum IS-10506 on repairing gastric mucosal injury expressed by Mucin 5AC (MUC5AC) induction from ketorolac in rodent model. In the experiment, 48 male Wistar rats were randomly assigned and segregated into four groups: the control group (K1), the group with ketorolac administration (K2), the group given L. plantarum IS-10506 after ketorolac administration (K3), and the group given L. plantarum IS-10506 before and after ketorolac administration (K4). Gastric tissue was examined for cells producing MUC5AC via immunohistochemistry. MUC5AC differences between groups were compared using Kruskal{\textendash}Wallis and Mann{\textendash}Whitney U tests. Significant differences were observed between each group on Days 5, 7, and 10 of necropsy (p=0.043; p=0.030; p=0.022). The ketorolac group (K2) consistently exhibited the lowest values during all examination days. Group K4 manifested a higher expression of MUC5AC relative to group K3. However, group K3 demonstrated a significantly increased from Day 1 to 10 of necropsy (p=0.030). Administering probiotic L. plantarum IS-10506 prior to ketorolac proved beneficial by significantly accelerate (p=0.030) the MUC5AC expression cells and gene expression.

}, doi = {10.5530/pj.2024.16.25}, author = {Mia Ratwita Andarsini and Andy Darma and Alpha Fardah Athiyyah and Khadijah Rizky Sumitro and Reza Gunadi Ranuh and Fauziah Pratiwi and Wibi Riawan and Widjiati Widjiati and Ingrid S Surono and Subijanto Marto Sudarmo} } @article {2396, title = {Effective Dose of Cocoa as a Preemptive Analgesic and Anti- Inflammatory Agent Assessed through Pain Scale and Tumor Necrosis Factor Alpha (TNF-α) in an Acute Pain Animal Model}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {October 2024}, pages = {1134-1137}, type = {Research Article}, chapter = {1134}, abstract = {

Background: Pain is a significant issue for 40-50\% of hospital patients, with 10-50\% of acute pain cases potentially progressing to chronic pain. Pain-associated inflammation often involves the release of mediators, including Tumor Necrosis Factor Alpha (TNF-α). Cocoa beans contain polyphenols, catechins, anthocyanidins, and proanthocyanidins, compounds believed to possess analgesic properties. This study aims to assess cocoa{\textquoteright}s potential as an oral preemptive analgesic agent in an acute pain mouse model, with an emphasis on its impact on inflammation through TNF-α levels. Methods: This true experimental study involved 24 male white mice split into four groups: a control group (K0) receiving a placebo, a treatment group receiving 15 mg/kg BW oral paracetamol (Kpct), a treatment group receiving 0.5 mg/g BW cocoa (K1), and a treatment group receiving 1 mg/g BW cocoa (K2). Pain response was measured using TNF-α levels and the von Frey test. The Kruskal-Wallis test and One-Way ANOVA were employed for statistical analysis. Results: Cocoa at doses of 0.5 mg/g BW and 1 mg/g BW substantially reduced TNF-α levels (75.82 {\textpm} 7.77 and 70.79 {\textpm} 11.50, respectively) compared to the control and paracetamol groups (98.22 {\textpm} 14.74 and 92.81 {\textpm} 2.64). On the first day, compared to the control group{\textquoteright}s 1.82 {\textpm} 0.78 von Frey values, the cocoa-treated groups{\textquoteright} values (6.20 {\textpm} 2.72 and 7.63 {\textpm} 4.11) were notably higher. There were no notable variations in von Frey values across the groups on the second day. However, a correlation was found between von Frey values on the first and second days. Conclusion: Cocoa can potentially serve as an effective preemptive analgesic agent, reducing pain and inflammation primarily by reducing TNF-α levels. These results provide validity to the use of cocoa as an alternative therapy in acute pain management.

}, keywords = {Cocoa, Pain, Pain Degree Analgesia, TNF-α}, doi = {10.5530/pj.2024.16.185}, author = {Herry Cahya Fathani and Herdiani Sulistyo Putri and Prananda Surya Airlangga and Christrijogo Sumartono Waloejo and Ira Sari Yudaniayanti and Pudji Lestari} } @article {2413, title = {The Effectiveness of Booklet and Images Game Education in Anemia Adolescent Women on Anemia Prevention Knowledge, Attitudes and Behavior}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {October 2024}, pages = {1183-1187}, type = {Research Article}, chapter = {1183}, abstract = {

Background : Risk factors for anemia in adolescents are lack of education, as well as adolescents inability to apply the information obtained in daily life. Effective health education improving adolescents knowledge, attitudes, and behaviors related to health. Objective : This study aims to test the effectiveness of Booklet and Image game education in adolescent girls on knowledge, attitudes, and behaviors to prevennt anemia. Participants were young women aged 15-19 years in the work area of the bayan Health Center and Gangga Health Center, who were selected through a screening process of 186 participants. each location had 93 respondents. Method : usiang a quasi-experimental two-group pre-test post-test design. In both groups, a pre-test was carried out furthermore, the intervention group was given health education through booklets and image game education, and the control group received health education with booklets. After the intervention was carried out, a pot-test was carried out on both groups. Results : Coherence analysis showed that the control group (booklet), adolescent girl with anemia who received the booklet and image game education in the intervention group reported better knowledge about anemia (p=0,000, n2 p=0,727)attitudes about anemia (p=0,000, n2 p=0,698), and behavior of adolescent girls with anemia between the intervention group and the control group were much different after being given with anemia between the intervention group and the control group were much different after being given booklets and image game education compared to adolescent girls who only received booklets. Conclusion : A multifaceted approach to health education can have a significant positive impact, especially when it involves interactive elements.therefore, the same educational program can be applied in various places to increase the effectiveness of a program or activity goal

}, keywords = {Adolescent women, Anemia, Attitudes, Behavior, Booklet, Image Game Education, knowledge}, doi = {10.5530/pj.2024.16.193}, author = {Misroh Mulianingsih and Suriah Suriah and Healthy Hidayanty and Ridwan Amiruddin and Veny Hadju and Dea Wulandari and Andi Ummu Salmah} } @article {2405, title = {Effects of Bacteriophage in Postoperative Endophthalmitis Caused by Staphylococcus aureus}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {October 2024}, pages = {1188-1191}, type = {Research Article}, chapter = {1188}, abstract = {

Postoperative endophthalmitis is a serious complication of cataract surgery. It may leads to vision loss. The most common organism cause endophthalmitis is gram-positive bacteria, mainly Staphylococcus aureus (S. aureus). To prevent postoperative endophthalmitis, eye drops or intracameral administration of antibiotic agents are universally used. In recent years, the trend of endophthalmitis treatment has grown rapidly. Administration of bacteriophage is a subject of research for the treatment and prophylaxis of postoperative endophthalmitis. This literature review investigates the potential of bacteriophage to provide a rapid, effective alternative to antibiotic treatments for postoperative endophthalmitis caused by S. aureus

}, keywords = {bacteriophage, postoperative endophthalmitis, Staphylococcus aureus}, doi = {10.5530/pj.2024.16.194}, author = {Herdina Ramadhani and Indri Wahyuni and Ismi Zuhria and Firman Setiawan and Annise Proboningrat and Djoko Legowo and Diah Indriani} } @article {2261, title = {Factors Affecting the Occurrence of Diarrhea in Infants Aged 7-14 Months in Puskesmas Alak Kupang City}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {388-392}, type = {Research Article}, chapter = {388}, abstract = {

Diarrhea is a disease characterized by changes in the consistency of stools to liquid accompanied by an increase in the frequency of defecation more than usual \> 3 times / day accompanied by changes, with or without blood and or mucus. Factors that cause diarrheal disease are infections, infections can be caused by bacteria, parasites and viruses. Busy parents often pay less attention to health and the impact of an unhealthy environment. The three children have digestive problems, which are still considered a habit and can be cured without having to go to a health facility. The purpose of this study was to identify and analyze the factors that influence the incidence of diarrheal disease in infants aged 7-12 months at the Alak Health Center, Kupang City. The type of research used is an analytical survey with a case control design. The population in this study was divided into case populations, namely all infants aged 7-12 months totaling 137 infants and controls were infants aged 7-12 months who did not experience diarrhea totaling 8,868 infants. Determination of the size of the control sample using a ratio of 1:1 with the case sample so that the number of control samples is the same as the case sample, namely 37 infants and the total sample is 74 infants. The sampling technique is simple random sampling. Analysis of the data used is the chisquare test. The results showed that there was a significant relationship with a history of premature birth (p-value = 0.015 with an odds ratio = 3.434), low birth weight (p-value = 0.015 with an odds ratio = 4.563), exclusive breastfeeding (p-value = 0.015 with an odds value ratio = 3.434), immunization status (p-value = 0.004 with odds ratio = 4.265) and history of allergies (p-value = 0.004 with odds ratio = 4.083) with the incidence of diarrheal disease at the Alak Health Center, Kupang City. Suggestions that health center health workers need to be able to increase prevention efforts from an early age by approaching the community and providing concrete education related to the incidence of diarrhea.

}, keywords = {BBLR, Diarrhea, Exclusive Breastfeeding and Immunization Status, Premature}, doi = {10.5530/pj.2024.16.60}, author = {Deviarbi Sakke Tira and Sonyalin Buifena and Sigit Purnawan and Maria Magdalena Dwi Wahyuni and Marni Marni and Anwar Mallongi} } @article {2406, title = {HBOT2 Preconditioning Prolonged Inflammation After Decompression Diving}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {October 2024}, pages = {1192-1195}, type = {Research Article}, chapter = {1192}, abstract = {

Background: The mechanism involved in HBO2 preconditioning in preventing inflammation in diving is still unclear. Syndecan-1, which is an important part of glycocalyx, has never been studied for its involvement in HBO2 preconditioning to prevent inflammation in decompression diving. This study aims to determine how HBO2 preconditioning impacts inflammation through Syndecan-1, MDA, and IL-1a markers. Method: This study is a true experimental post-test design. Forty male 12- to 14-year-old Sprague Dawley rats were divided into four groups. HBO2 and decompression diving were carried out in an animal hyperbaric chamber. All data were collected 12 and 24 hours after the decompression diving. Result: The incidence of decompression sickness was less frequent in the HBO2 preconditioning treatment group as opposed to the control group (4 vs 9) but did not reach a significant level (p \> 0.05). All parameters showed no difference between the control and treatment groups 12 hours after the dive (p \> 0.05). Twenty-four hours after diving, the treatment group demonstrated substantially elevated IL-1a levels in comparison to the control group (p = 0.030), and the increase of IL-1a in the treatment group is significant (p = 0.001). Although MDA levels did not reach significant, the treatment group{\textquoteright}s increase in MDA levels 24 hours after diving was greater than that of the control group. Meanwhile, The treatment group had a smaller reduction in Syndecan-1 levels in comparison to the control group following diving 24 hours later. Conclusion: HBO2 preconditioning prolongs the inflammation, as evidenced by increased levels of MDA, Syndecan-1, and IL-1a, even though it can prevent decompression sickness. Further research is needed to find the right time and dose of HBO2 preconditioning to shorten the inflammation time.

}, keywords = {HBO2 preconditioning, IL-1a, MDA, Syndecan-1, VCAM-1}, doi = {10.5530/pj.2024.16.195}, author = {Sofia Wardhani and Aryati Aryati and Bambang Purwanto} } @article {2425, title = {Impact of Stigmasterol from Beluntas Leaves (Pluchea indica) on SGOT and SGPT Levels in Male Rats (Rattus norvegicus)}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {December 2024}, pages = {1311-1314}, type = {Original Article}, chapter = {1311}, abstract = {

Stigmasterol compounds have the potential as natural male antifertility drugs; based on the results of multiyear research and bioinformatics, further research is needed on the safety of stigmasterol preclinically. The study aimed to examine selected stigmasterol from blunt leaves that are safe for blood SGOT and SGPT levels the type of experimental research, the Post-test control design group. The study was conducted at the Biomedical Laboratory of the University of Muhammadiyah Malang, the Hufaka Bandung Laboratory, and the FKH Laboratory of Brawijaya University. The study was conducted from June to August 2024. The research sample of stigmasterol from blunt leaves came from the UPT materia medica of Batu City. The sampling technique was purposive random sampling. There were three dose treatments (0.125 mg/kgbb, 0.25 mg/kgbb, 0.5 mg/kgbb and control) with seven replications. Data analysis used one-way ANOVA and Duncan{\textquoteright}s further test. The results showed that giving various doses of stigmasterol could reduce SGPT in the blood but not SGOT. Duncan{\textquoteright}s test results of SGOT levels showed no significant difference between treatment categories; SGPT testing showed that the 0.125 mg/kgbb and 0.25 mg/kgbb treatments were not significantly different from the 0.5 mg/kg bb treatment but were substantially different from the control treatment. At the same time, the 0.5 mg/kgbb treatment was not significantly different from the other treatments. The conclusion and findings of the stigmasterol compound are in the safe category for use because it does not cause an increase in blood SGOT and SGPT and potentially tends to decrease.

}, keywords = {Antifertility, Beluntas, Men, Safety Test, Stigmasterol}, doi = {10.5530/pj.2024.16.211}, author = {Eko Susetyarini and Poncojari Wahyono and Sri Wahyuni and Endrik Nurrohman and Rahadian Zainul} } @article {2221, title = {Implementation Model of Acceptance of Covid-19 Booster Vaccine in Elderly in Bogor City}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {February 2024}, pages = {145-149}, type = {Research Article}, chapter = {145}, abstract = {

Objective: The major impact of COVID-19 occurs especially on the elderly, however the vaccination achievement rate among them tends to be low. The COVID-19 vaccination strategy for the elderly must still be implemented even though WHO has been revoked the Public Health Emergency of International Concern (PHEIC) status for COVID-19. One approach that can be taken is to empower the elderly in the vaccination process. This research aims to see the effectiveness of changes in knowledge, attitudes and behavior of the elderly regarding acceptance of the COVID-19 vaccine after receiving educational intervention using modules using the peer group method. Method: Quasi-experiment with a one-group pretest-posttest pattern. The research was conducted at the North Bogor Community Health Center in April-June 2023 on 66 elderly people taken using a purposive sampling technique. The questionnaire is used to look at characteristics, vaccine history, assess respondents{\textquoteright} knowledge, attitudes and behavior regarding the COVID-19 situation. Data collection went through three stages, namely pre-test distribution, peer group method using module-19 and post-test distribution. Bivariate analysis with dependent t-test using SPSS 23.0 software. Results: The majority of elderly people were aged 60-74 years (90.9\%) and there was a significant increase (p\<0.05) in the average score of knowledge, attitudes and behavior of elderly people before and after being given the intervention. The average knowledge score before intervention was 69.60 {\textpm} 9.51 and after intervention 80.30 {\textpm} 10.29, attitude score before intervention 80.43 {\textpm} 9.61 and after intervention 85.25 {\textpm} 11.99, and behavior score before intervention 86.29 {\textpm} 14.67 and after intervention 90.35 {\textpm} 10.34. Conclusion: implementation of the model with intervention modules is effective in increasing knowledge, attitudes and behavior. It is hoped that with this increase, elderly people will be able to receive the COVID-19 vaccine.

}, keywords = {Acceptance of the COVID-19 Vaccine, Attitudes, Behavior, knowledge, Module, Peer group}, doi = {10.5530/pj.2024.16.20}, author = {Astrid Dewi Prabaningtyas and Ridwan Amiruddin and A Arsunan Arsin and Healthy Hidayanty and Chatarina Umbul W and Ummu Salmah and Lalu Muh Saleh and Sudirman Nasir and Anwar Mallongi} } @article {2244, title = {The Influence of Workplace Spirituality and Leader Member Exchange on Organizational Commitment of Nurses at Elim Rantepao Hospital, North Toraja District}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {287-291}, type = {Original Article}, chapter = {287}, abstract = {

Background: The determining factor for the success of health service organizations today lies in how well management builds commitment, especially employee performance as internal customers. How well executives and all employees at all levels emphasize the importance of patient retention. How well customer commitment is translated into easy-to-understand actions, processes and performance records and how seriously leadership views all employees as a valuable investment and not a burden to the organization. This study aims to analyze the influence of Workplace Spirituality and Leader Member Exchange on Organizational Commitment in Nurses at Elim Rantepao Hospital, North Toraja Regency. The type of research is quantitative research using an observational study with a cross sectional study design. The sample was 102 nurses in the Inpatient Unit of Elim Rantepao Hospital, North Toraja Regency. Results: of the research show that organizational commitment in the Inpatient Unit of Elim Rantepao Hospital, North Toraja Regency is 46.1\% with the variable that has the most significant influence on nurses{\textquoteright} organizational commitment, namely Workplace Spirituality with a sig value of 0.001\<0.05 with a value of 51.1\%, as well as the most influential dimension. towards organizational commitment, namely Professional Respect with a sig value of 0.001 \<0.05 with a value of 46.6\%.

}, keywords = {LMX (Leader Member Exchange) and Organizational Commitment, Workplace Spirituality}, doi = {10.5530/pj.2024.16.42}, author = {Adrian Benedict Wijaya and Irwandy and A. Indahwaty Sidin and Noer Bahry Noor and Syahrir A Pasinringi and Sangkala and Anwar Mallongi} } @article {2202, title = {Inhibitory Mechanisms of Soybean Extract on the Development of Breast Cancer Through Modulation of Cellular Immune Response}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {February 2024}, pages = {01-08}, type = {Original Article}, chapter = {01}, abstract = {

Background: Breast cancer is the most frequently diagnosed cancer in women worldwide. Consumption of soy products has been reported to reduce the incidence of and mortality rate for some cancers, including breast cancer. However, there are limited in vivo studies on the inhibitory effect of soybean extracts on breast cancer. Objectives: To examine the effect of soybean extracts on breast cancer cellular immunity and to determine the role of CD4+ and CD8+ T cells in the development and outcome of breast cancer. Material and Methods: Rat were induced with DMBA 11 times to get a breast cancer model. A soybean extract was given at different doses starting one week before DMBA induction and continued until the end of the study. At the end of the study, peripheral blood was collected, and the lymphocytes were examined using flow cytometry. Results: The phytochemical screening of soybean extract, using the Q-TOF LC/MS method, detected four bioactive components from the isoflavone and saponin groups. The incidence of tumor formation in the NeC, SE-D250, SE-D500, and SE-D1000 groups was 100\%, 83\%, 33\%, and 33\%, respectively. The highest proportion of CD4+ T cells was found in the NeC (69.35\%), while the lowest was in the SE-D1000 (63.75\%). The highest and lowest proportions of CD8+ T cells were found in the SE-D1000 and NeC groups, at 35.95\% and 31.15\%, respectively. Conclusions: The soybean extract was able to reduce the incidence of breast tumor formation in DMBA-induced rat in a dose-dependent manner. The soy extract group{\textquoteright}s CD4+/CD8+ ratio was close to that of healthy rats compared to the DMBA-induced group without soy extract. A lowered CD4+/CD8+ ratio is followed by a lower risk of tumor formation.

}, keywords = {Breast cancer, CD4+, CD8+, Cellular immune response, Soybean extract}, doi = {10.5530/pj.2024.16.1}, author = {Andi Muh. Maulana and Kusmardi Kusmardi and Erni Hernawati Purwaningsih and Andon Hestiantoro and Taifo Mahmud and Heri Wibowo and Bambang Pontjo Priosoeryanto and Primariadewi Rustamadji and Numlil Khaira Rusdi} } @article {2292, title = {Inventory of Medicinal Plants for Male and Female Reproductive Health by Ethnic Javanese and Ethnic Dayak Seberuang in Balai Harapan Village, Tempunak District, Sintang Regency}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {June 2024}, pages = {545-553}, type = {Original Article}, chapter = {545}, abstract = {

Introduction: Knowledge of the use of plants as medicine is one of the local wisdom of the Javanese and Dayak ethnic communities of Seberuang, Balai Harapan Village, which has been passed down from generation to generation by their ancestors. This research aims to inventory plants that have medicinal properties for male and female reproductive health which are used by the people of Balai Harapan Village, especially the Javanese and Dayak Seberuang ethnic groups. Methods: This research used a triangulation technique which is a combination of interviews, observation, and documentation of plant types for male and female reproductive health. The data obtained in this research analyzed and presented narratively for each plant and accompanied by pictures and tables. Results: There are 26 types of medicinal plants used by the Javanese and Dayak Seberuang ethnic groups to support reproductive health. Conclusion: The plants used as medicine for male and female reproductive health by the Javanese and Dayak Seberuang ethnic groups in Balai Harapan Village come from several families, namely Zingiberaceae, Asteraceae, Poaceae, Lamiaceae, Fabaceae, Polypodiaceae, Rubiaceae, Caricaceae, Melastomataceae, Gleicheniaceae, Dilleniaceae, Phylanthaceae, Blechnaceae, Schizaeaceae, Piperaceae, Moraceae, and Asparagaceae. The most common types of plants used come from the Zingiberaceae family.

}, keywords = {Local wisdom; Medicinal plants; Reproduction health}, doi = {10.5530/pj.2024.16.86}, author = {Ruqiah Ganda Putri Panjaitan and Risma Nurkholifah and Eko Sri Wahyuni and Kurnia Ningsi and Liya Angga Lestari} } @article {2436, title = {Natural Cartilage-Derived Scaffolds for 3D Mesenchymal Stem Cell Culture: Promoting Chondrogenesis and Modulating Secretome Composition}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {December 2024}, pages = {1365-1372}, type = {Research Article}, chapter = {1365}, abstract = {

Introduction: The regenerative potential of stem cells lies in their secretome, a collection of signaling molecules whose composition is shaped by the cellular microenvironment and external stimuli. To enhance cartilage regeneration, a 3D culture system using a natural cartilage scaffold has been developed to create a more chondrogenic secretome. This study investigates the chondrogenic differentiation of mesenchymal stem cells (MSCs) cultured in a decellularized bovine cartilage scaffold and analyzes the resulting secretome{\textquoteright}s composition. Methods: This study employed a randomized time series design to examine MSCs chondrogenic differentiation. A control group was cultured in standard 2D conditions, while two experimental groups were cultured in either 2D medium supplemented with chondrocyte differentiation medium (positive control) or a 3D decellularized bovine cartilage scaffold. The study hypothesized that the 3D culture would promote chondrogenesis at least as effectively as the positive control. Key chondrogenic markers were evaluated at various time points. Results: Statistical analysis revealed significant differences in marker expression between the experimental and control groups. SOX-9 and aggrecan were elevated in both experimental groups. The 3D group showed higher RUNX-2 expression and the highest Coll-2 expression at later time points. Additionally, growth factor analysis showed the 3D group had the highest levels of IGF-1 and FGF-2 towards the end of the study. Conclusion: 3D culture of MSCs in a bovine cartilage scaffold enhances chondrogenic differentiation and produces a secretome with comparable chondrogenic potential to traditional 2D culture with differentiation medium, suggesting its promise for cartilage regeneration.

}, keywords = {Cartilage, Culture Technique, Secretome, Stem Cells, Three-Dimensional}, doi = {10.5530/pj.2024.16.220}, author = {Kukuh Dwiputra Hernugrahanto and Naufaldy Rifqiaulia Noerda and Jifaldi Afrian Maharaja Dinda Sedar and Lukas Widhiyanto and Dwikora Novembri Utomo and Djoko Santoso} } @article {2280, title = {Nephrogenic Diabetes Insipidus or Bartter Syndrome? A Dilemma of Refractory Hypokalemia in Pregnancy: A Case Report from Soedono Regional Public Hospital in Madiun}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {495-497}, type = {Case Report}, chapter = {495}, abstract = {

Hypokalemia is a rare condition in pregnant women. Muscle weakness and life-threatening heart damage can occur if the baseline condition is not treated, especially if it recurs and persists. The differential diagnosis in recurrent hypokalemia includes nephrogenic diabetes insipidus, characterized by the kidneys{\textquoteright} inability to respond to vasopressin to concentrate urine, or Bartter syndrome, a tubulopathy resulting from a rare genetic mutation affecting the loop of Henle, leading to potassium wasting. A 24-year-old primigravida at 35/36 weeks gestation presented with refractory hypokalemia, indicative of diabetes insipidus or a differential diagnosis of Bartter syndrome. The patient complained of limb weakness combined with polydipsia, polyuria, and a history of periodic paralysis due to severe hypokalemia since 2016. She consistently took potassium supplements and monitored her potassium levels. Since becoming pregnant in November 2021, the patient has been hospitalized three times due to limb weakness, with the lowest serum potassium level recorded at 1.6. Throughout her pregnancy, fetal growth and development remained within normal limits, and her blood pressure ranged from 100-120/60-80. In patients with limb weakness, evaluating serum potassium levels is crucial. In this case, the suspicion of nephrogenic diabetes insipidus could not be confirmed due to the unavailability of antidiuretic hormone (ADH) tests. Bartter syndrome, although rarely encountered, should be considered in patients with recurrent hypokalemia. The challenge in Indonesia lies in the lack of specific gene examinations for diagnosis, making diagnostics relatively difficult. Our recommendation for cases like this is to conduct ADH examinations and thoroughly investigate refractory hypokalemia.

}, keywords = {ADH, Bartter Syndrome, Diabetes insipidus, Hypokalemia, Pregnancy}, doi = {10.5530/pj.2024.16.79}, author = {Bayu Aditya and Manggala Pasca Wardhana and Tauhid Islamy} } @article {2279, title = {Pectoralis Block in Geriatric Patients with Breast Cancer Undergoing Modified Radical Dextra Mastectomy}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {492-494}, type = {Case Report}, chapter = {492}, abstract = {

Regional anesthesia has the advantage of minimal use of drugs capable of depressing cardiovascular or pulmonary function, especially in geriatric patients. The pectoral nerve block represents a regional procedure associated with fewer adverse effects compared to alternative regional anesthetic approaches. A 74-year-old woman with a weight of 35 kg and a height of 145 cm, was diagnosed with breast cancer. The patient complained of a lump in the right breast since the last 1 year, the lump felt hard and didn{\textquoteright}t feel painful. We conducted PECS I and PECS II blocks as part of the surgical protocol for dextra Modified Radical Mastectomy (MRM), with the primary goal of minimizing the requirement for opioids and anesthetic agents in the context of geriatric patients. In the PECS I block, we introduced a needle into the anatomical plane situated between the pectoralis major and pectoralis minor muscles, followed by the injection of 10 mL of 0.5\% ropivacaine. In the case of the PECS II block, we administered a 20 mL dosage of ropivacaine at the third rib level above the serratus anterior muscle to ensure a wide distribution of the local anesthetic within the axillary region. The complete PECS block procedure typically spans a duration of approximately 30 minutes. The block was smooth and did not show complications, during the duration of the opioid, fentany 25 mcg was added during the axillary lymph node dissection. VAS scores at first 12 and 24 hours, 1 and 1. Thus, the PECS block can be used as an analgesic either during or postoperatively. The use of PECS 1 and 2 blocks in radical mastectomy may reduce the need for opioids for intraoperative and postoperative pain.

}, keywords = {Breast cancer, Geriatrics, Modified Radical Mastectomy (MRM), PECS Block}, doi = {10.5530/pj.2024.16.78}, author = {Ardhian Wardana and Dedi Susila} } @article {2276, title = {Phytochemical Analysis of Curry Leaf Extract (Murraya koenigii L.) as a Potential Animal Feed and Medicinal Ingredient}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {471-477}, type = {Research Article}, chapter = {471}, abstract = {

Herbal plants have been used for centuries as traditional medicine to treat various diseases. Green plants generally contain phytochemical compounds, such as vegetables and plants that add aroma to dishes, one of which is curry leaves (Murraya Koenigii). This research aims to identify the phytochemical compounds contained in curry leaves. This research was carried out from August 2023 to October 2023. The curry leaves that were obtained were converted into extract form and then the extract was tested for the content of alkaloids, flavonoids, saponins, phenolics and tannins. The research results showed that curry leaf extract contained 23.73\% alkaloids, 1.24\% flavonoids, 8.74\% saponins, 4.4\% phenolics, and 5.2\% tannins. Alkaloids in plants have a role as a defense against biotic and abiotic disorders. The benefits of flavonoids in plants include anti-mutagenic, anti-inflammatory, antioxidant and anti-carcinogenic. Saponins have various benefits in the health sector, including being able to reduce cholesterol concentrations in the blood. Polyphenols have good antioxidant power because this group can provide electrons to neutralize free radical electrons formed in the body. Tannins also consist of polyphenolic compounds which have antibacterial, antioxidant and astringent activities. The results of the analysis regarding the content of secondary metabolite compounds in curry leaf extract play an important role in the development of future medicines and need to be carried out to provide knowledge to the public. This study can be a basis for bioactive content for further research to expand the use of medicinal plants in the future, especially curry plants.

}, keywords = {Curry leaf extract, Human health, Medicine, Phytochemicals, Plant}, doi = {10.5530/pj.2024.16.75}, author = {Mohammad Anam Al Arif and Sunaryo Hadi Warsito and Mirni Lamid and Widya Paramita Lokapirnasari and Aswin Rafif Khairullah and Siti Rani Ayuti and Sugito and Intan Permatasari Hermawan and Oky Setyo Widodo and Rakhi Gangil} } @article {2361, title = {Phytochemical Screening and Antibacterial Activity Test of Ethanol Extract of Durian (Durio Zibethinus murr.) Soya Varieties Against Pathogen Bacteria Escherichia Coli in Raw Drinking Water}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {933-941}, type = {Research Article}, chapter = {933}, abstract = {

Introduction: Durian (Durio zibethinus) fruit skin contains antibacterial compounds. The antibacterial content in durian skin (Durio zibethinus) such as alkaloids, flavonoids, saponins, phenols and tannins can inhibit the growth of pathogenic bacteria such as Escherichia coli, Salmonella typhosa and Staphylococcus aureus and act as a disinfection agent. This research aims to determine the secondary metabolite compounds and antibacterial activity of the ethanol extract of Soya durian peel against the pathogenic bacteria Escherichia coli ATCC 25922. Methods: This type of research is a laboratory experiment, including making Soya durian peel extract using the maceration method using 96\% ethanol solvent. The antibacterial activity test was carried out using the liquid dilution method to determine the Minimum Inhibitory Concentrations (MIC) value and the solid dilution method to determine the Minimum Bactericidal Concentration (MBC) value. Results: Based on qualitative phytochemical screening, Soya durian peel ethanol extract contains secondary metabolite compounds with an average content of 4.24\% alkaloids, 22.95\% flavonoids, 1.74\% saponins, 57.41\% phenols and 2.27\% tannins. Soya durian peel extract has an MIC against E. coli ATCC 25922 bacteria of 3.12\%, while the MBC value of Soya durian peel extract against E. coli ATCC 25922 bacteria is 6.25\%. The results of the One Way ANOVA analysis of the Minimum Bactericidal Concentration (MBC) data have a significant value of 0.00 \< 0.05. The results of the Pearson correlation test (r) showed a significant number of 0.000 (p \< 0.05), the Pearson correlation coefficient between concentration and number of bacterial colonies was (r) = 0.812. This means that the higher the concentration of Soya durian peel ethanol extract given, the less the number of E.coli ATCC 2592 bacterial colonies will be reduced. The results of a simple linear regression test showed that the value of Y = 245.618 {\textendash} 29.016 245,618 colonies and each increase in the concentration of Soya durian peel ethanol extract by 1\% will cause a decrease in the number of bacterial colonies to 29,016 colonies. Conclusion: Soya durian skin extract has antibacterial compounds that can kill pathogenic Escherichia coli bacteria in raw drinking water.

}, keywords = {Antibacterial, Durian (Durio zibethinus) Soya variety, Escherichia coli, Phytochemicals, raw drinking water.}, doi = {10.5530/pj.2024.16.151}, author = {Rahwan Ahmad and Ridwan Amiruddin and A.Arsunan Arsin and Stang Stang and Hasanuddin Ishak and Wahiduddin and Gemini Alam and Bambang Wispriyono and Anwar Mallongi} } @article {2254, title = {The Potential of Orthosiphon Aristatus Extract in Improving Skin Lesions in Atopic Dermatitis: A Mice Model Study}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {342-347}, type = {Research Article}, chapter = {342}, abstract = {

Introduction: This research discusses the potential of Orthosiphon aristatus, a medicinal plant, in improving skin lesions in Atopic Dermatitis by regulating Th2 cytokines and showing anti-allergic activity. Methods: The study was conducted using BALB/C mice induced with DNCB for 21 days to create an atopic dermatitis model. Then, the mice were orally administered Orthosiphon aristatus extract for 14 days (after 7 days of induction). The doses given were divided into six groups: 17.5 mg/kgbw, 35 mg/kgbw, 70 mg/kgbw, and 140 mg/kgbw. Molecular levels such as IgE, IL4, IL22, and PGE2 were analyzed from blood samples. In addition, the severity of skin lesions was assessed morphologically, and histological examination was performed to confirm clinical improvement. Results: This study showed that the administration of Orthosiphon aristatus extract reduced the severity of skin lesions in all intervention groups. Histologically, this extract reduced epidermal thickness and mast cell infiltration. These findings were also supported by dose-dependent decreases in IgE, IL4, IL22, and PGE2 levels. So, the conclusion of this study is that Orthosiphon aristatus extract shows potential for improving skin lesions in atopic dermatitis by regulating Th2 cytokines and demonstrating anti-allergic activity in a mice model with DNCBinduced atopic dermatitis. Conclusion:\ The administration of Orthosiphon aristatus extract clinically and histologically exhibits anti-atopic effects that can be explained through the regulation of molecules such as IgE, IL4, IL22, PGE2, and NO. Orthosiphon aristatus has the potential to be a valuable herbal therapy for managing atopic dermatitis.

}, keywords = {Atopic dermatitis, Orthosiphon aristatus, Skin lesions, Th2 cytokines}, doi = {10.5530/pj.2024.16.52}, author = {Thigita Aga Pandaleke and Kusworini Handono and Dhelya Widasmara and Hani Susianti} } @article {2400, title = {Probabilistic Simulation and Sensitivity of Health Risks from Nickel and Cobalt Deposition Around the Mine}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {October 2024}, pages = {1159-1163}, type = {Research Article}, chapter = {1159}, abstract = {

Background: Mining activities can produce large amounts of heavy metals that are discharged uncontrollably, causing widespread ecosystem pollution. Heavy metal pollution is a serious problem, as heavy metals are toxic and non-degradable, polluting air, water and soil. Although some heavy metals are considered essential for normal physiological functions, concentrations that exceed the prescribed limits can have negative impacts on human health and biota. Exposure to heavy metals can cause toxicity to various tissues, organs and systems such as circulatory, respiratory, endocrine, immune, nervous, urinary and reproductive systems. Due to the differences in toxicity of heavy metals, risk assessment is very important in environmental management. Objective: To describe the probabilistic simulation and sensitivity of health risks due to Nickel and Cobalt deposition around the mine using monte carlo simulation. Methods: The method used in this study was literature review. Data collection was done through searching online databases such as: Pubmed and Science Direct. The keywords used in the article search were "Heavy Metal Toxicity", "Heavy Metal Effects", "Heavy metal risk analysis" and "Monte Carlo Simulation". Inclusion criteria were quantitative or qualitative research, target population was heavy metals Nickel and Cobalt, published in the last 10 years (2013-2023) articles in English and articles in original, fulltext and open access. Exclusion criteria are articles that are not relevant to the topic of discussion. Results: 14 articles were found, of which 3 articles on diseases or health problems caused by nickel, 3 articles on nickel risk analysis, 5 articles on diseases or health problems caused by cobalt, 3 articles on cobalt risk analysis. Conclusion: The environment around the mine shows a high level of toxicity, caused by the presence of heavy metals. This can result in serious environmental pollution and pose a great threat to human health. Chronic exposure to heavy metals can cause various health problems, such as mutagenesis, carcinogenesis, teratogenesis, deformation and organ damage.

}, keywords = {Cobalt, Health risk, Heavy metal contamination, Monte Carlo Simulation, Nikel}, doi = {10.5530/pj.2024.16.189}, author = {Sherly Wulandari and Anwar Mallongi and Budimawan and Agus Bintara Birawida and Nur Nasry Noor and M. Alimin Maidin} } @article {2291, title = {Renal Resistive Index and Estimated Glomerular Filtration Rate in Nondiabetic Chronic Kidney Disease Stage 3: A Cross-Sectional Analytical Study}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {June 2024}, pages = {538-544}, type = {Original Article}, chapter = {538}, abstract = {

Background: Renal resistive index (RRI) and estimated glomerular filtration rate (eGFR) are predictive markers for chronic kidney disease (CKD) progression. Aim: To evaluate RRI value, eGFRcr-cys and renal biomarker in nondiabetic patients with CKD stage 3 in Bangkok, Thailand. Methods: A cross-sectional analytical analysis was conducted involving nondiabetic patients with CKD stage 3, aged 35-85 years. Ultrasound was used to assess the RRI of arteries in both kidneys. Patients underwent assessments of serum and urine 24-hour. Results: Among the 61 participants (67.2\% male; mean age 69.03 {\textpm} 12.59 years), the mean eGFRcr-cys was 41.63 {\textpm} 8.64 mL/min/1.73 m{\texttwosuperior}, and the mean RRI was 0.65 {\textpm} 0.06. Patients were categorized into three RRI groups: low (\<0.65, n=35), intermediate (0.65-0.70, n=14), and high (\>0.70, n=12). The high RRI group showed a mean RRI of 0.73 {\textpm} 0.05 (p \< 0.01). Among those with high RRI group were significant decreased right kidney size (p\<0.05) and they had a lower BMI, averaging 22.49 {\textpm} 3.48. An increase in PP (59.66 {\textpm} 13.84, p=0.04) was also significant in this group. The correlations coefficient of RRI value showed a significant positive correlation with age (p\<0.05) and significant negative with BMI (p\<0.05). In addition, eGFRcr-cys displayed a significant negative correlation with UAGT and 24hUP (p\<0.05) and a significant while eGFRcr-cys positive correlation with both kidney size and urine iNOS(p\<0.01). Conclusion: An increase in RRI is inversely linked to age, BMI, and PP. Lower eGFR is correlated with factors that cause CKD progression.

}, doi = {10.5530/pj.2024.16.85}, author = {Patnaree Wongmanit and Kusuma Sriyakul and Parunkul Tungsukruthai and Ouppatham Supasyndh and Pratya Phetkate} } @article {2277, title = {The Role of Neuron Specific Enolase, S100B, Glial Fibrillary Acidic Protein, and Myelin Basic Protein as Prognostic and Survival Values in Traumatic Brain Injury: Systematic Review and Meta-analysis}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {478-484}, type = {Review Article}, chapter = {478}, abstract = {

Background: The high number of accidents and traumatic brain injuries, especially in the productive age group, causes a lot of morbidity and mortality. A fast and accurate examination method is needed for the diagnosis and treatment of traumatic brain injury. Nerve damage biomarkers such as Neuron Specific Enolase, S100B, Glial Fibrillary Acidic Protein, and Myelin Basic Protein, have been used globally both for research and daily use to determine the severity of traumatic brain injury. Methods: Searches and journal searches were carried out from Science Direct, Scopus, Springer Link, and PubMed, with the keywords "Neuron Specific Enolase", "S100B", "Glial Fibrillary Acidic Protein", "Myelin Basic Protein", and "Traumatic Brain Injury {\textquotedblright}. Screening was carried out using PRISMA 2021 to look for studies that met the criteria and were of sufficient study quality according to the Newcastle-Ottawa Scale. Results: Twenty-three studies were collected and further grouped based on outcomes, both prognostic and survival outcomes. Neuron Specific Enolase, S100B, and Glial Fibrillary Acidic Protein values were higher in poor outcomes (all p values \< 0.001) and poor survival (all p values \< 0.001) in traumatic brain injury. Myelin Basic Protein was not significant in poor outcome (p = 0.35), but was higher in poor survival (p \< 0.001) in traumatic brain injury. Conclusion: Neuron Specific Enolase, S100B, and Glial Fibrillary Acidic Protein, can be used as markers for prognostic and survival value in traumatic brain injury. Myelin Basic Protein can be used as a marker for survival value in traumatic brain injury.

}, keywords = {Glial Fibriallary Acidic Protein, Myelin Basic Protein, Neuron Specific Enolase, Prognostic Value, S100B, Survival, Traumatic Brain Injury}, doi = {10.5530/pj.2024.16.76}, author = {Wibowo Artho Sutrisno and Prananda Surya Airlangga and Citrawati Dyah Kencono Wungu and Prihatma Kriswidyatomo and Hamzah and Bambang Pujo Semedi and Mahmudah} } @article {2219, title = {Screening and Identification of Metabolites from Sambiloto (Andrographis paniculata) Ethanol Extract for Pro-Inflammatory Cytokines Inhibitory through In Silico and In Vitro Approaches}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {February 2024}, pages = {131-140}, type = {Research Article}, chapter = {131}, abstract = {

Objective: Andrographis paniculata has long been a traditional medicinal plant in Indonesia. This study is intended to evaluate the anti-pro-inflammatory cytokines of 98\% ethanol extract of A. paniculata by in vitro and in silico approaches. Inhibition of pro-inflammatory cytokines is also one of the therapies in treating COVID-19. Methods: The molecular docking approach was utilized as a first screening to evaluate the potential for suppression of macrophage cell activation; an ADMET prediction test was performed to determine the pharmacological, pharmacokinetic, and toxicity as a therapeutic target. TNF-α, IL-1{\ss}, and IL-6 levels were measured using an ELISA method to investigate anti-cytokine pro-inflammatory activity in LPS-induced RAW 264.7 macrophage cells. LC-MS/MS was used to identify additional metabolite compounds. Results: Ethanol extract containing particular metabolites 14-Deoxyandrographoside and 14-Deoxy-17-hydroxyandrographolide inhibited TNF-α and IL-1β by 100\% and IL-6 by 85.59\%, respectively. While compared to the Dexamethasone molecule as a positive control, preliminary screening and ADMET prediction for the metabolite compound 14-Deoxyandrographoside exhibited relatively high binding stability to the CD14 receptor by -7.5 kcal/mol and was safe against various ADMET indications. Conclusions: This study reveals that the compound 14-Deoxyandrographoside in pure ethanol extract is a potential anticytokine agent candidate for treating pro-inflammatory cytokines, including COVID-19 infection.

}, keywords = {LC-MS/MS, Molecular docking, Pro-inflammatory cytokines, Sambiloto}, doi = {10.5530/pj.2024.16.18}, author = {Evul Winoto Lukito and Dyah Iswantini and Budhi Antariksa and Mohamad Rafi and Setyanto Tri Wahyudi} } @article {2408, title = {Transient Receptor Potential Vanilloid 1 in Acute Pain: A Literature Review}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {October 2024}, pages = {1196-1201}, type = {Review Article}, chapter = {1196}, abstract = {

Transient Receptor Potential Vanilloid 1 (TRPV1) is a protein that functions as a non-selective channel receptor that is widely expressed in skin tissue, including keratinocytes, peripheral sensory nerve fibers, and immune cells. Several structural features of TRPV1 are involved in heat-induced activation, where stimulation of TRPV1 elicits a burning sensation, reflecting the receptor{\textquoteright}s important role in pain. A TRPV1- mediated signalling pathway that functions as an endogenous pain resolution mechanism by inducing nuclear translocation of β-arrestin2 to minimize desensitization of μ-opioid receptors (MOR). TRPV1 agonists can reduce pain primarily by interfering with pain nerve conduction. Several TRPV1 antagonist drug candidates have failed in clinical trials because by interfering with the detection of the above-mentioned stimuli, they triggered serious side effects such as hyperthermia and painful impaired heat detection. In the case of agonists, systemic administration causes more severe side effects such as respiratory damage. Therefore, only topical preparations with limited effectiveness have been developed. The TRPV1 agonist capsaicin is currently the only one approved for the treatment of muscle, bone, neuropathic pain and migraine, and is only available as a low-concentration cream or as a transdermal patch.

}, keywords = {Agonis TRPV1, Capsaicin, Pain, transient receptor potential vanilloid 1}, doi = {10.5530/pj.2024.16.196}, author = {Dian Retno Mumpuni and Herdiani Sulistyo Putri and Prananda Surya Airlangga and Christrijogo Sumartono Waloejo} } @article {2431, title = {VDR and WNT/β-catenin Expression in Invasive Breast Carcinoma of No Special Type: Role and Prognostic Value}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {December 2024}, pages = {1349-1354}, type = {Research Article}, chapter = {1349}, abstract = {

Background: Breast cancer prognosis is closely related to tumor size (T stage). The Vitamin D receptor (VDR), found in about 80\% of breast cancer cases, inhibits tumor growth, promotes differentiation, and enhances apoptosis. The growth of tumor cells is linked to β-Catenin, an essential element of the Wnt signaling pathway. Both β-catenin and VDR affect breast cancer aggressiveness. This study explored their correlation with the T stage of invasive breast carcinoma of no special type. Methods: This research employed a cross-sectional design, applied on paraffin-embedded specimens from patients with invasive breast cancer of no special type (NST) who underwent modified radical mastectomy (MRM) at Dr. Soetomo General Academic Hospital from January 2019 to June 2023. The samples were categorized into four groups based on the T stage. Immunohistochemical staining was performed using VDR and β-catenin antibodies. This study used analytic statistical methods to examine differences and correlations among VDR and β-catenin. Results: VDR expression and T stages were significantly different and negatively correlated. Expression of β-Catenin revealed significant differences and had positive correlations with T stages. VDR and β-catenin expressed no significant negative correlation with T stages. Conclusion: The study found significant differences and correlations between VDR and β-catenin expression with T stages in invasive breast carcinoma of NST. Both β-catenin and VDR play crucial roles in breast cancer cell proliferation.

}, keywords = {Breast cancer, Invasive breast carcinoma of no special type, Vitamin D Receptor, β-catenin}, doi = {10.5530/pj.2024.16.217}, author = {Dewi Sartika Ari Wanda and Willy Sandhika and Ridholia} } @article {2438, title = {Visual Mapping and Future Direction of Marine Products Supplementary and Chemotherapy in The Treatment of Breast Cancer. A Bibliometric}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {December 2024}, pages = {1379-1388}, type = {Research Article}, chapter = {1379}, abstract = {

Marine products have gained attention for their potential benefits in the treatment of breast cancer, offering an alternative or supplementary approach to traditional therapies. While they are not intended to replace established medical treatments like chemotherapy or surgery, marine natural products have shown promise in providing symptom relief, enhancing the quality of life, and potentially improving treatment success for breast cancer patients. Studies have explored the use of marine products in conjunction with chemotherapy for their palliative care benefits and as adjuvants to conventional therapies. Marinederived compounds have been investigated for their anticancer properties, including apoptosis induction, anti-proliferative effects, and modulation of signaling pathways involved in breast cancer progression. These natural products offer a complementary avenue for managing breast cancer, potentially enhancing treatment outcomes, and addressing therapeutic challenges. The utilization of marine products in breast cancer therapy dates back to ancient times when various cultures recognized the therapeutic benefits of plants, herbs, and marine resources. The purpose of this study is to visually map and guide future research on supplementary marine products and chemotherapy in breast cancer based on bibliometric analysis

}, keywords = {Anticancer, Bibliometric, Breast cancer, Chemotherapy, Marine products, Visual mapping}, doi = {10.5530/pj.2024.16.222}, author = {Herin Setianingsih and Nasywa Zahra Sajida Tsuroyya and Prawesty Diah Utami and Riami and Nanang Wiyono} } @article {2278, title = {Vitamin D and Wound Recovery: Illuminating the Path to Enhanced Healing in Diabetic Patients}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {485-491}, type = {Review Article}, chapter = {485}, abstract = {

Wound healing is a highly coordinated biological event as a response to injured skin. It commonly takes 14 days for a wound to be completely healed. However, the duration of wound healing may vary between individuals due to certain factors. One major factor that delays the wound-healing process is Diabetes Mellitus. Delayed wound healing with poor prognosis commonly occurs in diabetic patients. Chronic hyperglycemia may affect macrophage polarisation, which is essential in the wound healing mechanism. The macrophage polarisation enables the pro-inflammatory M1 phenotype to switch to the anti-inflammatory M2 phenotype. Thus, pro-inflammatory M1 phenotype prevails persistently in diabetic wounds, while the anti-inflammatory M2 phenotype remains deficient. It results in significantly elevated levels of pro-inflammatory cytokines triggered by the M1 phenotype. Prolonged wound healing times increase the risk of infection, which can lead to more severe complications. Vitamin D is widely recognized for its essential role in regulating calcium levels and supporting bone health, as well as its positive effects on the immune system. This vitamin has the potential to skew macrophages towards the M2 phenotype and promote a regenerative and anti-inflammatory environment.

}, keywords = {Diabetes mellitus, Macrophage Polarisation, Vitamin D, Wound Healing}, doi = {10.5530/pj.2024.16.77}, author = {Aditya Chrisdianto and Prananda Surya Airlangga and Belindo Wirabuana and Regina Purnama Dewi Iskandar} } @article {2020, title = {Analysis Factors Affecting the Utilization of Antiretroviral Treatment Services in HIV Patients in Ambon City Puskesmas}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {424-428}, type = {Research Article}, chapter = {424}, abstract = {

Acquired Immune Deficiency Syndrome (AIDS) is a collection of symptoms that arise due to decreased immunity caused by HIV infection. Human Immunodeficiency Virus (HIV) is a type of virus that infects white blood cells which causes a decrease in human immunity. Efforts are being made at this time for people with HIV namely Antiretroviral treatment (ARV). This study aims to analyze the factors that influence the utilization of ARV treatment services in HIV patients at the Ambon City Health Center. This type of research is a quantitative analytic study with a cross sectional design. Respondents in this study were obtained using the Isaac and Michael method of 156 HIV patients from the Waihaong Health Center and Karpan Health Center in Ambon City using a questionnaire. Data processing was carried out using SPSS and for data analysis using statistical tests using descriptive statistical tests, comparative analysis tests and multivariate analysis tests. The results showed that there was no effect of knowledge on the use of ARV treatment in HIV patients at the Ambon City Health Center. Furthermore, there is an influence on the patient{\textquoteright}s actions, access distance, and peer and community support on the use of ARV treatment in HIV patients at the Ambon City Health Center. The action variable and access distance are the variables that have the strongest influence compared to the other variables.

}, keywords = {AIDS, ARV, HIV, Puskesmas, Utilization}, doi = {10.5530/pj.2023.15.66}, author = {Faradila Kilkoda and Balqis and Indar and Darmawansyah and Atjo Wahyu and Anwar Daud and Anwar Mallongi} } @article {1990, title = {An Analysis of Local Knowledge of Phit-Samdaeng in Yang Sisurat District, Maha Sarakham Province and The Medicinal Plants Used to Relieve Its Symptoms}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {259-265}, type = {Original Article }, chapter = {259}, abstract = {

Introduction: {\textquotedblleft}Phit-Samdaeng{\textquotedblright} is a Thai term often used to refer to an illness of eating unsuitable foods. However, its use in a diagnosis is limited due to the unclear definition and description. This study aimed to compare knowledge of Phit-Samdaeng symptoms and treatments in Northeastern folk medicine, Thai Traditional Medicine, and medicinal plants in the Yang Sisurat District of Thailand. Methods: Semistructured interviews and participatory observations were used to collect data from 12 folk healers. Questions were designed to obtain local knowledge of Phit-Samdaeng and medicinal plants. The data was analyzed using descriptive statistics, comparison and interpretation. Results: Some symptoms of Phit-Samdaeng in the nine scriptures of Thai Traditional Medicine, Tumraya Silajaruek Watprachethupon, and of Kin-Phit and Phit-Kaboon, in Tamraya Boran Isan, were similar in Yang Sisurat. However, the names were different. There were also differences in species and the recipes of herbal remedies among folk healers and in the textbooks. The most used plants to treat Phit-Samdaeng by folk healers were Clerodendrum paniculatum L. and Clausena wallichii Oliv. var. guillauminii (Tanaka) Molino. Only nine species had pharmacological effects relating to Phit-Samdaeng treatment. Conclusions: The knowledge of Phit-Samdaeng symptoms in Yang Sisurat District were consistent with the Northeast folk medicine and Thai Traditional Medicine. The most commonly used species were native plants of Thailand. However, information on the medicinal taste and property from scriptures and pharmacological evidence is lacking. Therefore, the pharmacological activity and toxicity of herbal remedies should be further investigated for efficacy and safety.

}, keywords = {Alternative medicine, Folk knowledge, Herbal medicine, Phit-Samdaeng.}, doi = {10.5530/pj.2023.15.37}, author = {Hatthapan Wipanso and Bhanubong Bongcheewin and Suppachai Tiyaworanant and Suksalin Booranasubkajorn and Natchagorn Lumlerdkij and Pravit Akarasereenont} } @article {2146, title = {Analysis of the Impact of Heat Stress on Embryo Development of Broiler: A Literature Review}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {964-968}, type = {Review Article}, chapter = {964}, abstract = {

Temperature is an important factor in embryonic development because temperature plays a role in determining the overall development of the embryo. The effect of high temperature on the embryo will cause the embryo to make a defense by diverting some of its energy to form thermal homeostasis, which is a physiological response. The purpose of this study was to analyze heat stress on embryonic development. This study used a systematic literature review (SLR) following PRISMA guidelines and gathered the sample of papers through the PubMed databases using keywords categorized as heat, stress, and embryo. There were 31 papers used as samples. The result showed that hyperthermia in chickens caused an increase in heart rate per minute as a result of temperature pressure. It affects the hatching percentage, hatching time, weight, and mortality rate in chicken embryos. In summary, heat stress has an impact on embryonic development heat stress gives an impact on the development of embryos.

}, keywords = {Chicken, Embryo Development, Food security, Health risk, Heat Stress.}, doi = {10.5530/pj.2023.15.179}, author = {Maslichah Mafruchati and Nor Hayati Othman and Akhmad Kusuma Wardhana} } @article {2162, title = {Conformity of Differential White Blood Cell (WBC) Examination Results on the Sysmex DI-60 And Sysmex XN-1000 at Prof. Dr. IGNG Ngoerah General Hospital, Denpasar, Bali}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {December 2023}, pages = {1059-1063}, type = {Original Article}, chapter = {1059}, abstract = {

Background: Sysmex DI-60 and Sysmex XN-1000 are automatic analyzers that can be used to check WBC differential counts. Ensuring the suitability of the WBC differential count results from the two tools can help in daily practice. Objective: Knowing the suitability of the WBC differential count examination results on the Sysmex DI-60 and Sysmex XN-1000. Method: Observational analytical research with a cross-sectional design. The data analyzed were differential WBC count data from samples of babies who underwent Complete Blood Count examinations on the Sysmex XN-1000 device and IT Ratio on the Sysmex DI-60 device without intervention (pre-classification results) at the Clinical Pathology Laboratory, Prof. Head overMarch 2023 {\textendash} April 2023. Results: 81 samples were analyzed. The neutrophil value on the Sysmex DI-60 and Sysmex vs 34.15\% {\textpm} 14.83\%, median monocytes 4.00\% (0.00-30.00)\% vs 13.05\% (1.30-34.40) \%, median eosinophils 3.50\% (0.00-31.00)\% vs 3.75\% (0.00-29.90)\%, and median basophils 0.75\% (0.00-16.00)\% vs 0.30\% (0, 00-2.90)\%. The eosinophil variable did not show a significant difference between the results of the Sysmex DI-60 and Sysmex XN-1000 examinations (p=0.081). The results of the correlation analysis showed that there was a significant, strong, positive correlation between Sysmex DI60 and Sysmex. Strong positive significance in the lymphocyte variable (r=0.818). However, no significant correlation basophil variable (r=0.044). Bland-Altman analysis shows that the most appropriate differential count examination is the eosinophil variable. Conclusion: Automatic WBC analyzers apply not only one examination method to detect WBC. The use of the Sysmex DI-60 and Sysmex XN-1000 provides automation for checking the WBC differential count with different working principles. In this study, there was only agreement between the differential count results for the eosinophil variable, so when operating these tools, intervention from a Clinical Pathologist was still required.

}, keywords = {DI-60, Differential Count, WBC, XN-1000}, doi = {10.5530/pj.2023.15.194}, author = {I Dewa Komang Agung Cahyadi and I Nyoman Wande and Ni Nyoman Mahartini} } @article {2019, title = {Development of Patient Safety Management Learning Model Based on Problem Based Learning Integrated Soft Skill Higher Level Thinking for Health Students in Samarinda}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {418-423}, type = {Research Article}, chapter = {418}, abstract = {

Background: Nursing students who participate in clinical practice in hospitals are not ready to provide quality care in maximizing patient safety. This study aims to develop a Problem Based Learning Patient Safety (PBLPS) conceptual model design that stimulates high-level thinking soft skills for students and finds PBLPS learning tools. Method: Research and Development (R\&D), which is a research method that aims to produce new products through a development process. The stages of the research method consist of 3 stages, namely: the preliminary study stage; model development stage; model testing stage. The research subjects were nursing students at tertiary institutions in Samarinda city who had taken patient safety management courses, totaling 162 people. Data were analyzed using the Wilcoxon test. Results: Patient safety goals have not been implemented properly by students who practice clinical practice in hospitals. Students state that case-based learning can improve both hard skills and soft skills. The design of the integrated Problem Based Learning (PBL) learning model and the integrated PBLPS learning tools developed are RPS and integrated PBL-based modules on patient safety management. The results of the analysis of the soft skills assessment of high-level thinking students who practice in hospitals in the first year and the second-year show that there are differences in the results of the assessment of high-level thinking soft skills in the application of patient safety. Conclusion: The Problem Based Learning Patient Safety (PBLPS) conceptual model can stimulate high-order thinking soft skills for students in patient safety management practices in hospitals.

}, keywords = {Patient Safety, Problem Based Learning, Soft skills.}, doi = {10.5530/pj.2023.15.65}, author = {Hilda and Supriadi and Hesti Prawita Widiastuty and Arsyawina and Anwar Mallongi} } @article {2011, title = {The Effect of Combination between Green Tea Extract and Curcumin Extract from Mt. Lawu on BAX, Bcl-2 and Caspase-3 in Cisplatin-Induced Rat Models}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {370-374}, type = {Research Article}, chapter = {370}, abstract = {

Introduction: The study determines effect of Combination between Green Tea and Curcumin Extract from Mount Lawu on BAX, Bcl-2 and Caspase-3 in Cisplatin (Cis)-induced rat models. Methods: We treated four rats in each group and randomly distributed them into four groups: group C (-) was the negative control group with no treatment given, group C (+) was the positive control group given Cis only, group A1 was given green tea extract and curcumin extract combination after Cis, and group A2 was given Ginkgo biloba after Cis. Expression levels of BAX, Bcl-2, and Caspase-3 were assessed by ELISA. An ANOVA, a parametric test, was used if the data were normally distributed. If there were significant differences between the three groups regarding BAX, Bcl-2 and Caspase-3, a post hoc test was performed to determine the differences between treatments. Results: The results of the study show that combination between green tea and curcumin extract can increase Bcl-2 levels with an average value of 15.42 + 0.76 ng/mL, better than Ginkgo biloba extract with a value of 13.50 + 0.47 ng/mL, reduce BAX and Caspase-3 levels with a value of 6.57 + 0.38 ng/mL and 2.89 + 0.19 ng/mL, better than Ginkgo biloba with a value of 7.34 + 1.06 ng/mL and 3.86 + 0.34 ng/mL. Conclusion: This research shows that Combination between Green Tea and Curcumin Extract can increase Bcl-2 levels and reduce BAX and Caspase-3 in Cis rat models after fourteen days of treatment, better than Ginkgo biloba.

}, keywords = {Antiapoptotic, Antioxidant, Ototoxicity.}, doi = {10.5530/pj.2023.15.57}, author = {Novi Primadewi and Harijono Kariosentono and Ari Probandari and Budiyanti Wiboworini} } @article {1970, title = {The Effect of Drying Methods on Chemical Profiling of Zingiberaceae Herbs Production}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {March 2023}, pages = {128-133}, type = {Research Article}, chapter = {128}, abstract = {

Background: Drying method is one of the important steps in post-harvesting in herbal production in order to prolong the shelf-life of herbal medicine and to ensure the good quality. Objective: This research aimed to profile and quantify the metabolites changing using metabolomic based on 1H-NMR Spectroscopy on Curcuma and Zingiber genus due to the drying process. Material and Methods: Five species of the Curcuma and Zingiber genus were Curcuma longa L., Curcuma aeruginosa Roxb., Curcuma xanthorrhiza Roxb., Zingiber officinale Roscoe. and Zingiber amaricans BI. The drying method applied were freeze drying and oven drying method at -1080C and 500C, respectively. Samples were extracted using buffer phosphate and deuterated methanol (3:7). The analysis was performed using 1H-NMR spectroscopy 400 MHz with TSP as an internal standard. The 1H-NMR spectra were analysed with multivariate (Principal Component Analysis). Results: The relative concentrations of sucrose and fructose at 5 samples in freeze drying method showed significant higher content than those in oven drying. On the other hands, glucose was mostly significant higher concentration in oven drying at 5 samples. Fructose and sucrose were significantly higher in freeze drying method than those in oven drying in Curcuma and Zingiber genera. Relative glucose content was higher in oven drying at Curcuma genus Conclusion: Therefore, oven drying method which applied heating at 500C is an appropriate method for herbal medicine production, especially in Curcuma and Zingiber genus, for maintaining the major secondary metabolites.

}, keywords = {Curcuma Genus, Freeze drying, Multivariate, Oven drying, Zingiber Genus.}, doi = {10.5530/pj.2023.15.17}, author = {Dinar Sari C. Wahyuni and Siti A. E. Astuti and Soerya Dewi Marliyana and Yudi Rinanto and Tiara Dewi S. Pratama and Ahmad Fauzi and Ari Satia Nugraha} } @article {2158, title = {Effect of Plant Stage and Solvent Extraction on Catechin Contents in Borrasus flabellifer L. Male Flower}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {December 2023}, pages = {1036-1041}, type = {Original Article}, chapter = {1036}, abstract = {

Borrasus flabellifer L. is a plant in the Arecaceae (Palmae) family with numerous bioactivities such as diuretic promotion, antioxidant, and antimicrobial effects. In this study, we are interested in the phytochemical compounds present in B. flabellifer L. male flowers. We investigated the effects of plant stage and solvent used for extraction. Initially, both the young and mature male flowers of this plant were extracted using water or ethanol. Several standard compounds, including gallic acid, catechin, caffeic acid, and quercetin, were utilized to screen the phytochemicals in these plant flowers. After analyzing the samples using High-Performance Liquid Chromatography (HPLC) method, only catechin was detected in all extracts, while the others were not detected (did not match the peak spectrum pattern compared with standard). Using a detection wavelength and curve parameters with an R2 value of 0.9999, catechin detection via HPLC was performed at 280 nm within the range of 13.12 to 210.00 {\textmu}g/mL detection. Regarding the stage of the male flower, the young ones extracted with water provided the highest catechin content at 6.65 {\textmu}g/mg extract, while the others fell within the range of 4.02 to 4.44 {\textmu}g/mg extract. Therefore, it has been confirmed that catechin can be found in B. flabellifer L., and water should be used as the solvent for extraction for further applications of this plant.

}, keywords = {Borrasus flabellifer L., Catechin, HPLC, Male flower, Mature, Young}, doi = {10.5530/pj.2023.15.190}, author = {Chayut Fongsuk and Patnaree Wongmanit and Kanoktip Pansuksan} } @article {2049, title = {The Effect of Safety Climate on Safety Behavior with Safety Motivation as An Intervening Variable on Contractor Workers in PT X}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {June 2023}, pages = {411-416}, type = {Research Article}, chapter = {411}, abstract = {

Safety climate in the workplace and safety behaviour of workers need attention to be able to assess what factors are the causes of safe action for workers. This study aims to determine the effect of safety climate on safety behaviour with safety motivation as an intervening variable on contractor workers at PT X. The type of research is analytical observational with a cross sectional design. Questionnaires were given to 380 respondents. The data were analyzed using path analysis to see the direct and indirect effects of safety climate on safety behaviour. The results showed, 361 respondents (95\%) with good safety climate, 367 respondents (96.9\%) with good safety motivation, and 348 respondents (91\%) with good safety behaviour. The path analysis showed that there was no direct influence of safety climate on safety behaviour in contractor workers at PT X (p value \>0.05). However, the safety climate indirectly affects safety behaviour through safety motivation (p value = 0.001). It is recommended to workers that they need to maintain and increase their motivation so the safe work behaviour will still create.

}, keywords = {Contractor workers, Path analysis., Safety behaviour, Safety climate, Safety motivation}, doi = {10.5530/pj.2023.15.93}, author = {Darius Tandiabang and Syamsiar S. Russeng and Lalu Muhammad Saleh and Atjo Wahyu and Yahya Thamrin and Anwar Mallongi} } @article {1994, title = {The Effect of Sapodilla Leaf Extract (Manilkara zapota L.) on Lipid Profiles of Alloxan-Induced Diabetic Mice}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {286-289}, type = {Original Article }, chapter = {286}, abstract = {

The aim of this study is to examine the effect of sapodilla leaf extract on lipid profiles of alloxan-induced diabetic mice. This research method are 30 male mice were used as experimental animals, which were randomly divided into five groups, each group consisting of 6 mice. The division of the group is as follows: Treatment of non-diabetic mice, diabetic mice, diabetic mice by administering pioglitazone at a dose of 2 mg/kg BW, extracts of manila sapodilla leaf (M. zapota L.) dose 100 mg/kg BW and 300 mg/kg BW. Observations were made on the 14th day, after administration of sapodilla leaf extract, mice were given light anesthesia and serum lipid profiles Total Cholesterol (TC), triglycerides (TG), High-Density Lipoprotein (HDL), and Low-Density Lipoprotein (LDL) were measured using diagnostic kits (Pars Azmoon Kit) and automatic analyzer (Abbot, model Alcyon 300). The results of this study are in TC, there was a significant difference in pioglitazone and 100 mg leaf extract against all treatment groups. In TG, there was a significant difference in pioglitazone and 300 mg leaf extract against all treatment groups. In LDL, there were significant differences in the 100 mg, 300 mg leaf extract and pioglitazone, against negative control, and diabetes control. In HDL, there was a significant difference in negative control and 300 mg leaf extract, against 100 mg leaf extract, pioglitazone, and diabetes control. The conclusions of this study exhibited the ethanol extract of M. zapota leaves contains several phytochemical compounds including alkaloids, flavonoids, saponins, polyphenols, tannins, quinones, and steroids. M. zapota leaves extract (100 mg/kg and 300 mg/kg) exhibited a significant effect on improvement in lipid protein.

}, keywords = {Alloxan, Diabetes, Lipid Protein, Manilkara zapota}, doi = {10.5530/pj.2023.15.40}, author = {Tridiganita Intan Solikhah and Tania Anggreani Wijaya and Salsabila and Daffa Alice Pavita and Ragil Kusnandar Miftakhurrozaq and Hartanto Mulyo Raharjo and Maya Nurwartanti Yunita and Faisal Fikri} } @article {2029, title = {The Effect Supplementation of Microbiota Inoculant in the Early Laying Hens Feed on High Density Lipoprotein (HDL) and Low- Density Lipoprotein (LDL) in Egg Yolk}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {June 2023}, pages = {270-273}, type = {Original Article }, chapter = {270}, abstract = {

Laying hens (Gallus gallus) are one of the poultries kept for their eggs. Eggs produced by chickens are used as a source of human food that contains a lot of nutrition and is affordable. Eggs also contain high levels of cholesterol in the yolk, so if consumed in large quantities, they will pose a risk of disease. This study aims to determine the effect of giving probiotics in the early layer laying hen feed on the levels of high-density lipoprotein and low-density lipoprotein in egg yolks. This study has four treatments, namely P1 = control, P2 = feed + AGP, and P3 and P4 = feed + probiotics. The probiotic used is a combination of three bacteria, namely Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium sp. The sample used in this study was egg yolk, which was collected in the last week of the study. Testing for HDL and LDL levels in egg yolks was carried out using the Quantitative Colorimetric/Fluorometric Determination of HDL and LDL/VLDL method. Testing HDL and LDL levels in this study showed that the administration of probiotics had an effect in the form of increasing HDL levels but did not have an effect in the form of a significant decrease in egg yolk LDL levels during the four-week treatment period in laying hens in the early layer phase, aged 18{\textendash}22 weeks. The best probiotic dose used among the three probiotic doses is 3 ml/kg of feed.

}, keywords = {High Density Lipoprotein, Laying hens, Low Density Lipoprotein, Probiotics.}, doi = {10.5530/pj.2023.15.73}, author = {Bodhi Agustono and Rini Apriliawati and Sunaryo Hadi Warsito and Maya Nurwartanti Yunita and Widya Paramita Lokapirnasari and Sri Hidanah and Emy Koestanti Sabdoningrum and Mohammad Anam Al-Arif and Mirni Lamid and Gandul Atik Yuliani and Shekhar Chhetri and Sarasati Windria} } @article {2045, title = {Factors Affecting the Long-Term Protection Against Hepatitis B Immunization in Infancy: A Meta-Analysis}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {June 2023}, pages = {385-394}, type = {Research Article}, chapter = {385}, abstract = {

Introduction: Hepatitis B virus (HBV) infection is a major global health issues and one of the most dangerous viral infections with a high mortality rate. Newborns and infant vaccination against chronic HBV infection are crucial for preventing mother-to-child transmission (MTCT). This study aimed to conduct a meta-analysis to investigate the factors affecting long-term protection against Hepatitis B Immunization in infancy. Material and Methods: Our literature searches are from PubMed, Science Direct, Web of Science, and ProQuest publications between January 2000 and December 2021. The included literature assessed the risk of bias using the Newcastle Ottawa Quality Assessment Scale. We identify Hepatitis B surface antibodies (anti-HBs) >= 10 mIU/mL as being protective against HBV infection. The results are combined with a random effect or fixed effect model. Results: Eighteen eligible observational studies with a total of 16,642 participants were included. Analysis of factors affecting long-term protection status by assessing anti-HBs titers showed significant results on several factors, including gestational age for anti-HBs titers (OR 2.5; 95\% CI 1.62-3.85; p\<0.0001), weight for age to anti-HBs titers (OR 1.36; 95\% CI 1.06-1.75; p=0.02), length for age to anti-HBs titers (OR 0.01; 95\% CI 0.01-0.02; p\<0.00001), and immunization status based on the number of vaccine doses (4 doses vs 3 doses) to anti-HBs titers (p\<0.00001). Conclusions: Anti-HBs titers of hepatitis B immunization were significantly affected by gestational age, weight for age, length for age, and vaccine doses. Parents of newborns must be informed about basic immunization and provide adequate nutritional intake to the mother and babies to prevent HBV infection.

}, keywords = {Hepatitis B antibodies, Hepatitis B vaccines, Immunity, Immunization, Infant.}, doi = {10.5530/pj.2023.15.89}, author = {Aldila Pratiwi and Bagus Setyoboedi and Citrawati D. K. Wungu} } @article {2156, title = {Factors Affecting the Occurrence of Diarrhea in Infants Aged 7-12 Months in Puskesmas Alak Kupang City}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {December 2023}, pages = {1024-1028}, type = {Original Article}, chapter = {1024}, abstract = {

Diarrhea is a disease characterized by changes in the consistency of stools to liquid accompanied by an increase in the frequency of defecation more than usual \> 3 times / day accompanied by changes, with or without blood and or mucus. Factors that cause diarrheal disease are infections, infections can be caused by bacteria, parasites and viruses. Busy parents often pay less attention to health and the impact of an unhealthy environment. The three children have digestive problems, which are still considered a habit and can be cured without having to go to a health facility. The purpose of this study was to identify and analyze the factors that influence the incidence of diarrheal disease in infants aged 7-12 months at the Alak Health Center, Kupang City. The type of research used is an analytical survey with a case control design. The population in this study was divided into case populations, namely all infants aged 7-12 months totaling 137 infants and controls were infants aged 7-12 months who did not experience diarrhea totaling 8,868 infants. Determination of the size of the control sample using a ratio of 1:1 with the case sample so that the number of control samples is the same as the case sample, namely 37 infants and the total sample is 74 infants. The sampling technique is simple random sampling. Analysis of the data used is the chisquare test. The results showed that there was a significant relationship with a history of premature birth (p-value = 0.015 with an odds ratio = 3.434), low birth weight (p-value = 0.015 with an odds ratio = 4.563), exclusive breastfeeding (p-value = 0.015 with an odds value ratio = 3.434), immunization status (p-value = 0.004 with odds ratio = 4.265) and history of allergies (p-value = 0.004 with odds ratio = 4.083) with the incidence of diarrheal disease at the Alak Health Center, Kupang City. Suggestions that health center health workers need to be able to increase prevention efforts from an early age by approaching the community and providing concrete education related to the incidence of diarrhea.

}, keywords = {BBLR, Diarrhea, Exclusive breastfeeding, Immunization Status, Premature}, doi = {10.5530/pj.2023.15.188}, author = {Deviarbi Sakke Tira and Sonyalin Buifena and Sigit Purnawan and Maria Magdalena Dwi Wahyuni and Marni Marni and Anwar Mallongi} } @article {2033, title = {Habitat Characteristics of Aedes Sp Larval Containers and Density of Container Index (CI) In the Area Endemic and Non-Endemic to DHF In Makassar City}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {June 2023}, pages = {290-295}, type = {Original Article }, chapter = {290}, abstract = {

Aedes sp has breeding habitats in water reservoirs or containers with relatively clear water, both inside and outside the house and in public places. The existence of containers as a breeding habitat for Aedes sp mosquitoes can increase the population density of Aedes sp mosquitoes, triggering high transmission of the dengu virus in the city of Makassar. The purpose of the study is to determine the characterisitic relationship of containers with the index of aedes sp larvae in dengue endemism areas in Makassar city in 2022. This study is an analytical observational study. Quantitative analysis with a cross-sectional approach. The sample sampling technique is a technique with simple purposive sampling. The sample was 600 houses in endemic and non-endemic areas in makassar city. The results of this study are container types with CI density in endemic areas, namely bucket 242 (CI = 9.5), while in non-endemic areas namely bucket 298 (CI = 5.4), container materials with CI density in endemic areas namely plastic 422 (CI = 10.9), while in non-endemic areas namely plastic 482 (CI = 5.6), and container locations with CI materials and densities namely in indoor locations with plastic material 398 (CI = 8.0), while in non-endemic areas with CI materials and densities, namely in indoor locations with plastic material 455 (CI = 5.5). The results of the chi-square test in the endemic area obtained a value of p = 0.478 which means that there is no relationship between the container type and the presence of aedes sp larvae in Tamamaung, Chi-square test results in non-endemic areas obtained a value of p = 0.217 which means that there is no relationship between the container type and the presence of Aedes sp larvae in the new Malimangan Village. The results of the chi-square test in the endemic area obtained a value of p = 0.000 which means that there is a relationship between the location and the presence of aedes sp larvae in tamamaung, and Meanwhile, the results of the Chi-Square test in non-endemic areas obtained a value of p = 0.631 which means that there is no relationship between the location and the presence of Aedes sp larvae in the new malimongan. There is no relationship between CI density and endemic and nonendemic regions. All six regions fall into the moderate category. His advice for the Makassar City Health Office, especially in Tamamaung, Pisang Utara, Pampang, Malimongan Baru, Kalukuang and Rappojawa villages, is that it is necessary to conduct counseling / information about the importance of environmental cleanliness and mosquito breeding sites to residents evenly and distribute abate, so that the community can be vigilant and avoid the transmission of dengue fever

}, keywords = {Characteristics of Container Habitats, Endemic and Non-Endemic., Larval Density}, doi = {10.5530/pj.2023.15.77}, author = {Muh Ichsan and Hasanuddin Ishak and Erniwati Ibrahim and Hasnawati Amqam and Isra Wahid and Syahribulan and Rusdiyah Sudirman and Anwar Mallongi} } @article {2111, title = {Immunostimulating Activity of Sungkai Leaf Stigmasterol Isolate against Cluster Differentiated 8+ T (CD8+T) from Covid 19 Disease Exposure}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {738-742}, type = {Original Article}, chapter = {738}, abstract = {

Objective: Covid 19 is a disease that infects cells in the airway lining the alveoli. This disease is caused by a virus named SarsCoV2. One way to handle it is to increase immunity. The body itself consists of immune system organs. One component of the immune system that is very important in protecting the body against the SARS-Cov-2 virus attack is Cluster of Differentiation 8+ T (CD8+T). CD8+T is one of the components of the adaptive immune system or often known as the specific immune system and is cytotoxic, so it is called Cluster of Differentiation8 +T. One of the plants used by the community to increase endurance is Sungkai (Peronema canescens Jack.). This study aims to see its activity against Cluster of Differentiation 8+ T (CD8+T). Methods: Sungkai leaf powder was extracted using 70\% ethanol solvent and evaporated using a rotary evaporator to obtain ethanol extract and fractionated with n-hexanes and isolated to obtain active isolates of sungkai leaves against the immune system. Isolates obtained from the isolation of the active fraction of n hexanes that have been tested as immunostimulants in previous studies. This study used test animals, namely male white mice of the wistar strain as many as 30 mice consisting of 5 heads for each group. Group I is a control group that is not given treatment and groups II, III, IV, V and VI are given exposure to the Sars CoV2 virus antigen that causes Covid 19 disease, namely using one of the Covid19 vaccines, namely the 0.0013 mL Moderna vaccine which is given Intra Muscular. Groups II, III, IV, V are treatment groups induced with Sars CoV2 antigen and given a test preparation, namely sungkai leaf isolate with 3 dose variations, namely 1, 10 and 100 mg/kg bw given orally, and group VI was given stimuno 50 mg/kgbw as a comparison. Results: From the results of immunostimulant testing of sungkai leaf isolate, it was found that stigmasterol has an immunostimulant effect in increasing Cluster of Differentiation 8 + T (CD8 + T) in the body given SARS-CoV-2 virus antigen. Significant results were seen in the 100 mg/kg bw dose group which increased CD8+T by 53.93 ng/dl. Based on statistical tests conducted in the form of ANOVA tests, CD8+T also showed significant differences with a significance value of \<0.05. Conclusion: Stigmasterol isolate of sungkai leaf has the effect of increasing Cluster of Differentiation 8+ T (CD8+T) levels in the body given exposure to SARS-CoV 2 virus antigens.

}, keywords = {Cluster of Differentiation 8+ T (CD8+T), Immunostimulant., SARS-CoV-2, Stigmasterol, Sungkai}, doi = {10.5530/pj.2023.15.145}, author = {Dwisari Dillasamola and Fatma Sri Wahyuni and Rauza Sukma Rita and Yufri Aldi} } @article {2128, title = {Implementation Analysis of COVID-19 Vaccination Policy in Southwest Maluku Regency}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {843-845}, type = {Research Article}, chapter = {843}, abstract = {

The implementation of the COVID-19 vaccination policy can be influenced by several factors, including communication, available resources, task disposition, and organizational structure. This study aims to analyze the factors affecting the implementation of these policies. This study employed a qualitative descriptive design, with data collection techniques including observation and interviews. The research was conducted in Southwest Maluku Regency in October-November 2022, with a total of 5 informants consisting of key informants, such as the Regent of Southwest Maluku as the chairman and spokesperson of the Task Force, ordinary informants, such as the Head of the Regional Disaster Management Agency, the head of Surveillance and Immunization at the District Health Office of Southwest Maluku, and the Coordinator of the COVID-19 Task Force Expert Team, as well as ordinary informants, such as community leaders. The results of the study revealed that the implementation of the COVID-19 Vaccination Policy in Southwest Maluku Regency has been well executed. The government has actively conducted health promotion and socialization, and has adequate resources equipped with specific competencies and expertise. However, there were several challenges during the implementation, such as the spread of misinformation, the difficult geographical terrain, and limited communication access to all areas. The findings of this study are expected to serve as an evaluation material for the government in the implementation of the COVID-19 vaccination program.

}, keywords = {COVID-19 Vaccine, Health promotion, Policy, Task Force., Vaccination Policy}, doi = {10.5530/pj.2023.15.161}, author = {Joska Rap Rap and Balqis and Sukri Palutturi and Indar and Masni and Atjo Wahyu and Anwar Mallongi} } @article {2067, title = {In Silico Study on the Potential of Guaiacol Extract from Green Tea (Camellia sinensis) as a Stimulant for Carbanoic Anhydrase II in Renal Tubular Acidosis}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {August 2023}, pages = {494-499}, type = {Original Article }, chapter = {494}, abstract = {

This study explores the potential of Guaiacol, a green tea extract from Camellia sinensis, as a stimulant in renal tubular acidosis through in-silico investigation on the Carbanoic Anhydrase II enzyme. Utilizing comprehensive computational tools including PyMOL, PyRx, Protein Plus, and the Lipinski{\textquoteright}s Rule of Five, a detailed examination of the molecular structure and its interactions with the target enzyme was conducted. The results from Protein Plus revealed interactions between Guaiacol and Carbanoic Anhydrase II. Quantitative parameters were determined with Binding Affinity values of -5, -4.7, and -4.5, along with RMSD values of 0, 0.956, and 1.412. The Lipinski{\textquoteright}s Rule of Five was employed to evaluate the compound{\textquoteright}s drug-like properties, with the findings indicating a molecular weight of 124, one hydrogen bond donor, two hydrogen bond acceptors, a log P of 1.4, and a molar reactivity of 34.65. Overall, these findings suggest that Guaiacol holds promising therapeutic potential in the treatment of renal tubular acidosis.

}, keywords = {Camellia sinensis., Carbanoic Anhydrase II, Guaiacol, Molecular docking, Renal Tubular Acidosis}, doi = {10.5530/pj.2023.15.108}, author = {Rahadian Zainul and Rismi Verawati and Agus Suprijono and Riso Sari Mandeli and Asri Peni Wulandari and Dony Novaliendry and Ritmaleni and Linda Rosalina and Muhammad Arya Ghifari and Amalia Putri Lubis and Viol Dhea Kharisma and Vikash Jakhmola and Maksim Rebezov and ANM Ansori} } @article {2068, title = {Molecular Docking of Thaflavine from Camellia sinensis in Inhibiting B-Cell Lymphoma Through BCl2 Apoptosis Regulator: An In Silico Study}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {August 2023}, pages = {500-505}, type = {Original Article }, chapter = {500}, abstract = {

This study aims to analyze the potential of Thaflavine, a compound found in green tea (Camellia sinensis), as an inhibitor in inhibiting B-cell lymphoma through its interaction with the BCl2 apoptosis regulator using an in-silico approach. The research methodology involved the use of software tools such as PyMOL, PyRx, Protein Plus, and the Lepinski Rule. Through molecular docking analysis using PyMOL and PyRx, the findings of this study demonstrate significant interactions between Thaflavine and BCl2, with Binding Affinity values of -5.5, -4.6, and -4.6, and RMSD values of 0, 1.436, and 2.292. The analysis using Protein Plus indicates the presence of interactions between Thaflavine and BCl2. Additionally, the analysis using the Lepinski Rule of Five reveals that Thaflavine meets the criteria as a potential drug compound, with a molecular weight of 549, 9 hydrogen bond donors, 12 hydrogen bond acceptors, a log P value of -2.5, and a molar reactivity of 119.17. The findings of this study provide important contributions to the development of therapies for B-cell lymphoma through an in-silico approach. However, further research is needed for in vitro and in vivo validation.

}, keywords = {Apoptosis Regulator BCl2, B-cell Lymphoma, Camellia sinensis., In-Silico Thaflavine, Molecular docking}, doi = {10.5530/pj.2023.15.109}, author = {Rahadian Zainul and Rismi Verawati and Herland Satriawan and Teresa Liliana Wargasetia and Devi Purnamasari and Amalia Putri Lubis and Bahrun and Riso Sari Mandeli and Muhammad Thoriq Albari and Viol Dhea Kharisma and Vikash Jakhmola and Maksim Rebezov and ANM Ansori} } @article {2169, title = {Novel Coumarin-Indole Hybrids as Cytotoxic Candidates: Synthesis and Antiproliferative Activity}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {December 2023}, pages = {1105-1111}, type = {Research Article}, chapter = {1105}, abstract = {

Cancer is regarded as a nightmare for humanity and a challenging task for medical professionals. Twelve hydrides (2a-2l), made of trifunctionalized coumarin and various substituted indoles, were created in an effort to realize the hope of a cancer cure. The 4,5-dimethoxysalicylaldehyde and ethyl acetoacetate were combined in a Knoevenagel reaction to create the coumarin component. The construction of the indole component involved converting various aminoindoles through diazotization and Sandmeyer reactions to twelve substituted indoles (1a-1l). These two components were combined through a Michael addition reaction to create the desired hybrids. Investigating their spectra released from various spectroscopical instruments allowed researchers to determine the 2D molecular frameworks of these hybrids. Studying the survival of nine tumor cell types after treatment with the synthesized hybrids enabled researchers to estimate there in vitro impact as cytotoxic candidates. By checking the cell viability using an MTT marker, it was possible to see that this effect was antiproliferative. The cytotoxicity measurements, IC50 scores, revealed a number of intriguing facts. To start, the synthetic hybrids displayed a relatively similar cytotoxic pattern against the cancerous cell lines under investigation. Second, compared to hybrids with chloride, hydroxyl, or methoxy substituents, fluorinated hybrids are more toxic to cancerous cells. Finally, hybrids with indole substituted at position-6 (2i-2l) have the highest cytotoxicity among those with indole functionalized at position-4 (2a-2d) or position-5 (2e-2h). From these facts, the authors concluded that hybrids with indole substituted at position-4 can represent potential candidates as antiproliferative applicants. Moreover, hybrid 2i may serve as a valuable model for creating potent anti-breast cancer therapies.

}, keywords = {Anti-breast cancer, Coumarin, Cytotoxicity, Indole, Michael addition, MTT}, doi = {10.5530/pj.2023.15.201}, author = {Sarah S. Ismael and Noor Ahmed M. Waheed and Seema Mahmood Kasim and Yasser Fakri Mustafa} } @article {2073, title = {Phytochemical Compounds Identification From 70\% Ethanol Extract of Arcangelesia Flava (L.) Merr Stems Using LC-MS/MS and In-Silico Molecular Docking Approach as Inhibitor Interleukin-1β}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {August 2023}, pages = {528-534}, type = {Research Article}, chapter = {528}, abstract = {

Arcangelisia flava (L.) Merr has been traditionally used to treat jaundice, liver disease, diarrhea, fever, and inflammation. Judging from its potential, scientific evidence of this plant extract as an inhibitor of interleukin-1β is still lacking. This study aims to investigate the phytochemical compounds present in the 70\% ethanol extract of Arcangelesia flava stems by LC-MS/MS and to elucidate the ligand-protein interactions through in-silico studies. The extract was found to contain alkaloids, flavonoids, furanoditerpene, hydroxyquinoline, phenylpropanoid, phenol, and fatty acids. According to molecular docking of the 15 compounds analyzed by LC-MS/MS, the compounds 3-hydroxy-3{\textquoteright},4{\textquoteright},5{\textquoteright}-trimethoxyflavone (ΔG=-7.72 kcal/mol), fisisaine (ΔG=-6,91 kcal/mol), and demethyleneberberine (ΔG=-6.85 kcal/mol), which demonstrated the highest affinity for binding to the protein target. In addition, active amino acids contribute to this interaction by creating strong hydrogen bonds, such as MET148, LYS 103, and THR300. Phytochemical compounds from Arcangelesia flava may serve as adjunctive therapy or a promising source of advanced structures in drug discovery for treatments targeting interleukin-1β

}, keywords = {Arcangelisia flava (L.) Merr, Inhibitor interleukin-1β, LC-MS/MS, Molecular Docking.}, doi = {10.5530/pj.2023.15.114}, author = {Rizki Rahmadi Pratama and Irawati Sholikhah and Sukardiman and Ram Kumar Sahu and Retno Widyowati} } @article {2078, title = {Risk Analysis of Microplastic Exposure Through Consumption of Anadara Granosa at Coastal Area}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {August 2023}, pages = {558-562}, type = {Original Article }, chapter = {558}, abstract = {

Plastic waste is starting to threaten marine waters, especially microplastics. This micro-sized plastic can be consumed by marine biota. The accumulation of microplastics in the bodies of marine biota can threaten humans who consume them. The purpose of this study was to analyze the risk level of microplastic (polystyrene) exposure through the consumption of blood clams in the coastal area of Pao Village, Tarowang District, Jeneponto Regency. This type of research is a descriptive analysis with the Environmental Health Risk Analysis (EHRA) approach. The samples in this study were 30 respondents and 125 blood clams (anadara granosa). Data were obtained by interview using a questionnaire, identifying MPs in shells in the laboratory, and identifying polymer types using FTIR-spectroscopy. The results showed that 68 MPs of microplastics were found in blood clams (anadara granosa) samples. The dominant shapes were lines and blue. The types of polymers from the FTIR results are Polystyrene (PS), Low-density polyethylene (LDPE), Polyvinyl chloride (PVC), and Polyethylene (PE). The average non-carcinogenic daily intake (Intake) is 0.00012 mg/kg/day, the average risk level (RQ) is 0.0006, and the daily intake value is <=0.2 (RfD Styrene), so it is said to be safe and the risk level value (RQ) <= 1, then categorized as the risk of exposure to MPs in humans through consumption of blood clams is still categorized as safe. The route of exposure to MPs in humans is not only through blood clams, so efforts to control the risk of exposure to MPs in humans are still needed.

}, keywords = {Anadara Granosa., Blood clams, Microplastic, Polystyrene, Risk analysis}, doi = {10.5530/pj.2023.15.119}, author = {Nurhayati Namira and Anwar Daud and Anwar Mallongi and Hasnawati Amqam and Atjo Wahyu and Irwandy} } @article {2021, title = {The Risk Analysis of Patient Transfer Practice Events to Safety of Nurses and Patients of the TNI AU Dodi Sardjoto Hospital}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {429-432}, type = {Research Article}, chapter = {429}, abstract = {

Work accidents can occur due to unsafe action and unsafe condition factors. This type of research is a combination of qualitative and quantitative research (mix method) with a phenomenological approach that aims to determine the risks of patient transfer practices to the safety of nurses and patients. This research was conducted at the TNI AU Dody Sardjoto Hospital with a total sample of 51 people, the sampling technique is simple random sampling. Then in-depth interviews will be conducted on key informants, ordinary informants, and additional informants. Quantitative data analysis using chi square test and multiple linear regression. The results of the study found that the history of disease (p = 0.010), physical activity (0.026), facilities and infrastructure (0.011), SOP (p = 0.000), and the environment (p = 0.000) influenced the safety of nurses. The results also showed that compliance with SOP (Standard Operating Procedure) was the most influential variable with B of 0.675. It is also known that the results of the path test have an indirect influence between nurse safety and patient safety is E2 = 0.887 or 88.7\%. It is hoped that the results of this study can be a reference to reduce the risk of patient transfer practice events to the safety of nurses and patients.

}, keywords = {Accident, Patient Transfer, Work safety}, doi = {10.5530/pj.2023.15.67}, author = {Muh. Nur Amal Sali and M. Furqan Naiem and Masyitha Muis and Syamsiar S. Russeng and Atjo Wahyu and Erniwati Ibrahim and Anwar Mallongi} } @article {2149, title = {Safety Assessment of Supplementation with Cymbopogon citratus Stapf. (Lemongrass) Extract in Patients with Chronic Kidney Disease Stage 3: A Preliminary 90-Days Prospective Study}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {December 2023}, pages = {976-986}, type = {Original Article}, chapter = {976}, abstract = {

Background: Chronic kidney disease is a major public health issue, and due to resource constraints, many can{\textquoteright}t access dialysis. C. citratus, lemongrass is globally prevalent and known to increase urine output without toxicity. Objective: To examine the safety of C. citratus in patients with CKD stage 3. Materials and Methods: The major compound of C. citratus was analyzed using high-performance liquid chromatography (HPLC). 64 patients were enrolled and randomly assigned to control or C. citratus groups. The control group received a placebo, whereas the C. citratus group received 900 mg of C. citratus daily for 90 days. All participants were examined BUN, Scr, Cys-C, and eGFR, liver functions, RBC, HCT, Na+, K+, Cl-, and HCO3, and other biochemical parameters. Results: HPLC showed C. citratus contains of phenolic compounds. Clinically, C. citratus group had no notable side effects on T-Bil, AST, ALT and ALP. Also, maintained eGFR, SCys-C, K+ and Cl level. The level of blood Na+ was significant increase at day 30 (p \< 0.05). The control group had a significant decrease in eGFR and HCO3 levels (p \< 0.05) and a significant increase of Cl- and SCys-C. In addition, no statistical differences had found between groups in eGFR, BUN, Cr, Na+, K+, HCO3, PO4, RBC and HCT levels. Throughout the 90 days, no drug allergies or side effects were reported. Conclusion: Dietary supplementation with C. citratus may have a favorable effect on delaying the course of CKD and is safe to use for patients with CKD stage 3.

}, keywords = {Chronic Kidney Disease, CKD stage 3, Cymbopogon citratus Stapf., Lemongrass, Safety}, doi = {10.5530/pj.2023.15.181}, author = {Patnaree Wongmanit and Parunkul Tungsukruthai and Pratya Phetkate and Daraporn Rungprai and Sucharat Tungsukruthai and Ouppatham Supasyndh and Kusuma Sriyakul} } @article {2101, title = {Spatial Distribution of Microplastic Contamination in Blood Clams (Anadara granosa) on the Jeneponto Coast, South Sulawesi}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {August 2023}, pages = {680-690}, type = {Research Article}, chapter = {680}, abstract = {

Plastic waste in oceans will threaten of marine biota. There is a very high degree of plastic pollution in Indonesia, however the distribution of microplastic contamination in seafood, particularly that ingested by the general populace as a daily meal from seas, is quite low. The purpose of this investigation is to map the distribution of microplastic contamination in blood clams (Anadara granosa) throughout the South Sulawesi coast. Observational study employing a spatial analysis exploratory design. Blood clams (Anadara granosa) and information on wind, currents, and tides are used to map the distribution of microplastic pollution. The Kriging model was used for their geographical analysis. Purposive sampling was used to collect a total of 25 samples of blood clams (Anadara granosa) from offshore locations, residential waterways, mangrove ecosystems, rivers, and river estuaries. The results showed that the highest distribution of microplastic contamination was found in mangrove ecosystem areas and residential waters, while the lowest was found in river areas. Blood clams (Anadara granosa) have high levels of microplastic contamination because of sources of plastic pollution that come from waste disposal activities. It became stuck in the fisheries and mangrove ecosystems along the coast. Oceanography has an impact on how currents and winds migrate from the northeast (where mangrove habitats are) to the north (where residential waters are), carrying microplastic particles. Provision of waste management facilities by the local government and using the depuration method to eliminate microplastics in blood clams (Anadara granosa) consumed by the community needs to be done to reduce the impact on ecology and health.

}, keywords = {Blood Clams (Anadara granosa), Coastal Coast., Microplastic Contamination, Spatial Analysis}, doi = {10.5530/pj.2023.15.137}, author = {Rachmat Saleh and Anwar Daud and Hasanuddin Ishak and Hasnawati Amqam and Atjo Wahyu and Stang and Agus B. Birawida and Anwar Mallongi} } @article {1976, title = {Tectona Grandis Leaves: Determination of Total Flavonoid Content, Phenolic Content, Characterization of the Leaves, and Compound Identification in GC-MS}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {March 2023}, pages = {165-170}, type = {Research Article}, chapter = {165}, abstract = {

Nowadays, traditional plant study has grown in importance as their use has been increased. Tectona grandis (teak) is one of several plants that have been studied for its phytochemical and pharmacological properties. This plant includes a number of secondary metabolites, which may explain its diverse pharmacological properties. Although teak leaf compounds have been examined in the past, there is still little information on the diversity of teak leaf compounds. As a result, we are employing several methodologies to determine the total flavonoids content, phenolic content, water extractable matter, ethanol extractable matter, total ash content, and other component analysis of teak leaves. We found that the average of total flavonoid content in the teak leaves is about 3.93 {\textpm} 0.008\%w/w, while the average total phenolic concentration in teak leaves is about 4.3{\textpm}0.15\%w/w. The Gas Chromatography- Mass Spectrometry GC-MS study of the methanol extract of T. grandis leaves also found twenty-three active chemical compounds (phytochemical components)

}, keywords = {Ash content, Flavonoids content, GC-MS., Moisture content, Phenolic content, Teak, Tectona grandis, Water content}, doi = {10.5530/pj.2023.15.24}, author = {Pepi Budianto and Suroto Suroto and Brian Wasita and Diah Kurnia Mirawati} } @article {1945, title = {Antibacterial Effect of Nigella sativa L. Seed from Indonesia}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {1029-1032}, type = {Research Article }, chapter = {1029}, abstract = {

Nigella sativa L. was allegedly to has antibacterial effect. The aim of this research is to investigate antibacterial effect of ethanolic extracts of 96\% Nigella sativa L. from Indonesia toward Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus epidermidis. The Antibacterial activity of of Nigella sativa L toward Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus epidermidis using the well method. The 96\% ethanolic extract of 12,5; 25;50 and 100\% (mg/ml)inhibit growth of Staphylococcus aureus (with inhibition zone 11.06; 29.58; 28.22; 30.84 mm respectively) and Streptococcus epidermidis (20.90; 31.90; 29.93; 33.07 mm respectively). Ethyl acetate fraction of 96\% ethanolic extract of Nigella sativa concentration of 6,25; 12,5; 25;50 and 100\% (mg/ml) inhibit growth of Staphylococcus aureus (12.91; 15.06; 19.19; 37.48; 46.18 mm respectively and Streptococcus epidermidis (19.07; 19.21; 20.22; 21.62; 40.00 mm respectively). Ethanolic 96\% extract and ethyl acetate fraction of ethanolic extract of Nigella sativa have antibacterial effect toward Staphylococcus aureus and Streptococcus epidermidis invitro.

}, keywords = {Antibacterial, Nigella sativa L., Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus epidermidis.}, doi = {10.5530/pj.2022.14.206}, author = {Em Sutrisna and Sri Wahyuni and Aris Fitriani} } @article {1941, title = {Antioxidant, Antimicrobial, and Antiplasmodial Activities of Sonchus arvensis L. Leaf Ethyl Acetate Fractions}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {993-998}, type = {Research Article }, chapter = {993}, abstract = {

Infection is one of the health problems and a disease that mainly causes death. Malaria is a parasitic infection that is transmitted through the Anopheles sp. The female then causes infection and besides malaria, other contaminants that caused infection are bacteria such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antioxidant, antimicrobial, and antiplasmodial activity of Sonchus arvensis L. ethyl acetate fractions. In vitro antiplasmodial activity was carried out by Rieckman methods against Plasmodium falciparum strain 3D7. In vitro antioxidant activity was conducted by Prieto method against (1,1-diphenyl-2-picrylhydrazyl (DPPH). Then antimicrobial activity was performed using well diffusion method against Escherichia coli and Staphylococcus aureus. Maceration of S. arvensis L. dried leaves used n-hexane and ethyl acetate successively. Then the ethyl acetate extract was fractionated by vacuum column chromatography, using n-hexane and ethyl acetate as mobile phases. There are five fraction groups based on thin-layer chromatography (TLC) analysis. The IC50 of antioxidant and antiplasmodial activity showed that fraction IV was the lowest value and categorized as active for antioxidant (IC50=22.56 μg/mL), for antiplasmodial (IC50=12.07 μg/mL). Fraction IV also had antimicrobial activity, with diameter of inhibition zone (DIZ) of 19.22 mm against Escherichia coli and 17.167 mm against Staphylococcus aureus.

}, keywords = {Biological activities, Escherichia coli., Malaria, Plasmodium falciparum, Sonchus arvensis L., Staphylococcus aureus}, doi = {10.5530/pj.2022.14.202}, author = {Dwi Kusuma Wahyuni and Anindya Nariswari and Agus Supriyanto and Hery Purnobasuki and Hunsa Punnapayak and Wichanee Bankeeree and Sehanat Prasongsuk and Wiwied Ekasari} } @article {1841, title = {Bioactive Compounds from Purslane (Portulaca oleracea L.) and Star Anise (Illicium verum Hook) as SARS-CoV-2 Antiviral Agent via Dual Inhibitor Mechanism: In Silico Approach}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {352-357}, type = {Original Article }, chapter = {352}, abstract = {

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the COVID-19 pandemic that infects humans and attacks the body{\textquoteright}s immune system. The purpose of the study was to identify the potential of bioactive compounds in purslane (Portulaca oleracea L.) and star anise (Illicium verum Hook) via a dual inhibitor mechanism against SARS-CoV-2 proteases with an in silico approach. The samples were obtained from PubChem and RSCB PDB. Antivirus probability prediction was performed on PASS Online. Virtual screening was performed with PyRx via molecular docking. Visualization was used by PyMol and Discovery Studio. Compounds with the best antiviral potential are indicated by the low binding affinity value to the target proteins, namely SARS-CoV-2 TMPRSS2 and PLpro. The results showed that purslane luteolin has the best antiviral potential. However, further studies are required to validate this computational prediction.

}, keywords = {Antiviral agent, Illicium verum Hook, in silico, Portulaca oleracea L., SARS-CoV-2}, doi = {10.5530/pj.2022.14.106}, author = {Nur Sofiatul Aini and Viol Dhea Kharisma and Muhammad Hermawan Widyananda and Ahmad Affan Ali Murtadlo and Rasyadan Taufiq Probojati and Dora Dayu Rahma Turista and Muhammad Badrut Tamam and Vikash Jakhmola and Dony Novaliendry and Riso Sari Mandeli and Budhi Oktavia and Muhammad Thoriq Albari and Saddam Al Aziz and Muhammad Raffi Ghifari and Okta Suryani and Putri Azhari and Muhammad Arya Ghifari and Devi Purnamasari and Agariadne Dwinggo Samala and Mirella Fonda Maahury and ANM Ansori and Rahadian Zainul} } @article {1731, title = {Correlation Pattern of oxLDL, cortisol, hsCRP, and Adiponectin Levels in Atherosclerosis Risk Population-Based on Framingham Risk Score}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {February 2022}, pages = {14-20}, type = {Original Article}, chapter = {14}, abstract = {

Background: The central pathology of cardiovascular disease (CVD) is atherosclerosis. Therefore, it is necessary to examine proteins involved in the and CVD disease mechanism to predict the occurrence of cardiovascular disease due to atherosclerosis. Purpose: This study analysed the correlation pattern of hsCRP, oxLDL, cortisol, and adiponectin levels in atherosclerotic risk population based on the Framingham Risk Score (FRS) to determine the risk of atherosclerosis. Methods Participants were selected using the purposive sampling method,158 participants classes were fired into three risk groups according to FRS. Blood samples were collected, a hsCRP, oxLDL, cortisol, and adiponectin levels were measured using Enzyme-linked Immunosorbent Assay (ELISA). Results and Discussion: Using the inner model test result, four significant direct relationships are formed, indicated by p-value\< 0.000. It was FRS to oxLDL, cortisol adiponectin, cortisol to oxLDL, and oxLDL to adiponectin. Based on the indirect effect analysis, it is known that the indirect effect of FRS on Adiponectin through the increase in hs-CRP levels (0.211), FRS on Adiponectin increases in OxLDL levels is significant (-0.224). The coefficient of the effect of the FRS on Adiponectin levels through an increase in OxLDL levels is negative, and also FRS on Adiponectin. The indirect effect of FRS on Adiponectin is that the indirect effect and is not significant (4.083) through the increase in cortisol levels. Conclusion: All variables used in this study are correlated with each other. FRS with hsCRP and adiponectin form a relationship that directly affects each other. Meanwhile, FRS affect adiponectin through OxLDL and cortisol.

Key words: Adiponectin, Atherosclerosis, Cardiovascular disease, Framingham Risk Factor, hsCRP.

}, doi = {10.5530/pj.2022.14.3}, author = {Kumboyono Kumboyono and Indah Nur Chomsy and Fitria Nugraha Aini and Titin Andri Wihastuti} } @article {1925, title = {Differences in interleukin-6 and interleukin-17 expression in covid-19 post-mortem lung tissue biopsy compared with noncovid- 19}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {887-892}, type = {Original Article }, chapter = {887}, abstract = {

Background: COVID-19 has spread rapidly around the world. It is necessary to study lung tissue of postmortem COVID19 patients to determine the molecular alteration particularly the role of IL-6 and IL-17 in causing fatality. Objective: This study aims to determine the differences in the expressions of IL-6 and IL-17 in lung tissue of post-mortem COVID-19 patients compared to non-COVID-19 patients. This study also aimed to analyze the correlation between the expressions of IL-6 and IL-17 in lung tissue of post-mortem COVID-19 patients. Methods: This research is an observational analytic study with crosssectional approach. The samples were 15 paraffin blocks of post-mortem lung tissue biopsy of COVID-19 patients, and 15 paraffin blocks of inflammatory lung tissue biopsy or surgery of non-COVID-19 patients. IL-6 and IL-17 expressions were evaluated by immunohistochemical procedure. Result: There was a significant difference in the expression of IL-6 in the COVID-19 group and the non-COVID-19 group with a p-value = 0.001 (p \< 0.05). There was a significant difference in the expression of IL-17 in the COVID-19 group and the non-COVID-19 group with p-value = 0.001 (p \< 0.05). There was a significant correlation between the expressions of IL-6 and IL-17 in the COVID-19 group, with the Spearman coefficient value (rs) of 0.548 with p = 0.034 (p \< 0.05). Conclusion: There are differences in the expression of IL-6 and IL-17 between COVID-19 and non-COVID-19 lung tissue. There is a significant correlation between the expressions of IL-6 and IL-17 in post-mortem lung tissue of COVID-19 patients.

}, keywords = {Biopsy, COVID-19, IL-17, IL-6, Post mortem lung tissue.}, doi = {10.5530/pj.2022.14.184}, author = {Etty Hary Kusumastuti and Priangga Adi Wiratama and Grace Ariani and Stephanie Natasha Djuanda and Alphania Rahniayu and Nila Kurniasari and Dyah Fauziah and Anny Setijo Rahaju and Isnin Anang Marhana and Alfian Nur Rosyid and Dwi Wahyu and Gilang Muhammad Setyo Nugroho and Adhitri Anggoro and I Komang Rusgi Yandi and Bambang Pujo Semedi and Jilientasia Godrace Lilihata and Ummi Maimunah and Supriadi and Achmad Lefi and Lalu Galih Pratama Rinjani and Edi Suyanto and Ricardo Ardian Nugraha} } @article {1746, title = {Does Soybean (Glycine max) Supplementation Increase Oocyte Viability, Decrease Oocyte Apoptosis and Improve Embryo Development Quality? An Experimental Study}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {February 2022}, pages = {133-140}, type = {Original Article}, chapter = {133}, abstract = {

Introduction: One of the important factors in Assisted Reproductive Technology (ART) is embryo quality that depends on oocyte quality. Maternal nutrition in form of soybean supplementation is thought to have benefits in oocyte quality. To determine the effect soybean supplementation to the embryo quality from oocyte side. Methods: This experimental study involved female mice from Swiss strain aged 6 weeks that were divided into two groups, group with soybean (soybean group/SG) and group without soybeans (pellet group/PG). Follicles were collected and denuded to get oocytes. The oocytes were stained with MitoTracker for assessing the mitochondrial membrane potential and TUNEL for assessing the apoptotic level. Colour intensity was assessed using a confocal microscope and determined using ImageJ software. Sperms were extracted surgically from the epididymis-vas deferens and performed preparation prior to intra cytoplasmic sperm injection (ICSI) procedure. Then, the embryos were cultured and observed for the quality. Result: In TUNEL test, the average colour intensity of the SG was lower compared to the PG, significantly (p=0.03). While in the Mito Tracker test, the average colour intensity for the SG was higher compared to PG, not significantly (p=1.08). In addition, the embryo development on Day-1 and Day-3 showed more good embryo quality of SG compared to PG, significantly (p=0.03). Conclusion: Soybean supplementation improved embryo quality at the cleavage stage by decreasing apoptosis of the oocytes rather than increasing the viability the oocytes.

Key words: Oocyte, Soybean, Apoptosis, Embryo development, Embryo quality.

}, doi = {10.5530/pj.2022.14.18}, author = {Silvia W Lestari and Khairunnisa F Ilato and M. Iqbal A Pratama and Husna Qonitah and Gito Wasian and Amandanu Bramantya and Firda A Husna and Ria Margiana and Gita Pratama} } @article {1755, title = {The Effect of Curcumin and Virgin Coconut Oil Towards Cytokines Levels in COVID-19 Patients at Universitas Sebelas Maret Hospital, Surakarta, Indonesia}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {February 2022}, pages = {216-225}, type = {Research Article}, chapter = {216}, abstract = {

Introduction: To date, no specific therapeutic drug has been approved to target SARS-CoV-2. Hence, it remains a major challenge to decide what potential therapeutic regimens to treat COVID-19 patients. This study aims to investigate curcumin and virgin coconut oil (VCO) effects on cytokine levels (IL-1β, IL-2, IL-6, IL-18, TNF-α, and IFN-β) in COVID-19 patients. Methods: This study was a single-center, controlled trial with a parallel Arm or a Randomized Clinical trial design. A total of sixty COVID-19 patients admitted to the Universitas Sebelas Maret Hospital, Surakarta, Indonesia, were divided into two groups. The first group, consisting of 30 patients, was treated with Azithromycin 500 mg + Oseltamivir 2{\texttimes}75 mg + Hydroxychloroquine 400 mg/day for 5 days. The second group, comprising 30 patients, was treated with Azithromycin 500 mg + Oseltamivir 2{\texttimes}75 mg + Hydroxychloroquine 400 mg/day for 5 days, added with VCO 30 mL and curcumin 3{\texttimes}1 g/day for 21 days. The cytokine profiles of the serum samples were analyzed by the enzyme-linked immunosorbent assay (ELISA) on days 1, 14, and 21. Results: Our study showed that the second group had a significant reduction in IL-1β, IL-2, IL-6, TNF-α, and IFN-β levels after being treated with standard therapy added with curcumin and VCO on day 21 (p\<0.05). Conclusion: These results suggested that curcumin and VCO might benefit the treatment of COVID-19 patients.

Key words: COVID-19, Curcumin, Cytokines, Virgin coconut oil.

}, doi = {10.5530/pj.2022.14.27}, author = {Hartono and B Suryawati and Y Sari and A Avicena and Maryani and C Sukmagautama and H Apriningsih and L Shofiyah and RGH Novika and NJ Wahidah and NY Rahmawati and ANM Ansori and L Sumarno} } @article {1830, title = {Effect of Seahorse Extract (Hippocampus comes L.) on Caspase-3 and TUNEL assay in Rats After Depot Medroxyprogesterone Acetate Induction}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {253-258}, type = {Original Article }, chapter = {253}, abstract = {

Seahorse (Hippocampus spp) is marine fish and have pharmacological activity, such as an antiinflammatory, antioxidative, antifatigue and improve the fertility. Depot medroxyprogesterone acetate (DMPA) is a contraception drug for male and affect the endocrine system by inhibiting pituitary gonadotropin with reduce testosterone levels in 12 weeks. There are limited studies reported the effects seahorse extract (SE) on Caspase-3 and TUNEL assay in rats induced by DMPA. Thirty Sprague-Dawley (SD) male rats that were induced by 1.25mg/kgbw DMPA in 0 and 12 weeks. The animals were randomly into five groups, following: aquadest (G1), CMC 1\% (G2), SE dose of 150 mg/kgbw (G3), SE dose of 225 mg/kgbw (G4), SE dose of 300 mg/kgbw (G5). The rats were gavage every day from seven until week eighteen. On the last week, we taken the right and left testis to observed the apoptotic on Caspase-3 and TUNEL assay. Apoptotic marker was observed through immunohistochemistry from testicular tissue and analysed with plugin ImageJ IHC profiler, which is H-score as the results. Data were analysed using One-Way ANOVA and Bonferroni{\textquoteright}s post hoc tests. The SE decrease the Caspase-3 and TUNEL assay expression in rats induced by DMPA until eighteen weeks, with dose 150 mg/kgbw given the significant difference with p=0.028; \<0.05 and p=0.000; \<0.01. These results suggest that SE decreased germ cells apoptotic in DMPA induced rats.

}, keywords = {Apoptotic, Caspase-3, DMPA, Seahorse, TUNEL assay}, doi = {10.5530/pj.2022.14.93}, author = {Trisnawati Mundijo and Yurnadi Hanafi Midoen and Franciscus D. Suyatna and Agung Eru Wibowo and Kusmardi Kusmardi} } @article {1840, title = {Immunostimulating Study of Active Agent Fraction from Sungkai (Peronema canescens Jack.) Leaf from SARS-COV-2 Virus Antigen Exposure to NK and CD8+T Cells}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {344-351}, type = {Original Article }, chapter = {344}, abstract = {

Introduction: Sungkai (Peronema canescens Jack.) plant had been used as an immune system enhancer. Aim: In this study, the effect of Sungkai leaf extracts from 4 different fractions, namely n-hexane, ethyl acetate, butanol and residual water with 3 variations in doses of 1,10 and 100 mg/kg bw on the activity of NK and CD8+T cells in male white mice that have been exposed to SARS-Cov-2 virus antigen was investigated. Methods: The experimental animals used were 60 animals divided into 12 groups with 14 days of treatment which had previously been induced with SARS-Cov-2 virus antigen (Moderna) and given with Sungkai leaf extracts for 14 days and evaluated on day 15. The evaluation results of NK cells concentrations sequentially were 2.96; 4.66; 5.38; 5.43; 4.05; 2.89; 3.56; 4.21; 2.88; 1.99; 2.07; 4.40; 3.21; 3.40; and 6.93 ng/ml. On the other hand, the evaluation results of CD8+T cells concentrations sequentially were 27.47; 28.96; 29.19; 27.90; 21.85; 25.79; 27.98; 23.50; 23.39; 26.56; 22.62; 25.19; 23,55; 26,75; and 29,69 ng/ml. One-way ANOVA and Duncan test were used for the data analysis. Results: The results showed significant increase of concentration (p\<0.05) towards concentration of NK cells in the butanol fraction at a dose of 1 mg/kg BW and CD8+T cells in the residual water fraction at a dose of 100 mg/kg BW. Conclusion: It can be concluded that fraction from sungkai (Peronema canescens Jack.) at doses of 1,10 and 100 mg/kg bw shows immunostimulatory activity.

}, keywords = {CD8+T Cells, NK Cells, Peronema canescens Jack., SARS-CoV-2}, doi = {10.5530/pj.2022.14.105}, author = {Dwisari Dillasamola and Fatma Sri Wahyuni and Rauza Sukma Rita and Dachriyanus and Yohanes Alen and Salman Umar and Yufri Aldi} } @article {1808, title = {In Silico Phytochemical Compounds Screening of Allium sativum Targeting the Mpro of SARS-CoV-2}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {604-609}, type = {Research Article}, chapter = {604}, abstract = {

The global pandemic of coronavirus disease is still widely spread across the world causing catastrophic effect in both human life and global economy. By the end of year 2021, it has caused a total of 5.437.636 deaths across the world. Indonesia has rich plant biodiversity including medicinal plants that may be used for combating the virus. One of the commonly used medicinal plants comes from Allium species and it has been proved to have antiviral activity. Conducting an in silico study, we screened bioactive compounds that came from Allium sativum to fight against coronavirus through the inhibition of 3CL-Pro, one of the major protease that have an active role for viral replication. Molecular docking of compounds from Allium sativum to 3CL-Pro resulting in the discovery of 5 compounds that have the best binding affinity to 3CL-Pro, which are squalene, 1,4-dihydro-2,3-benzoxathiin 3-oxide, 1,2,3-propanetriyl ester, trans-13-octadecenoic acid and methyl-11-hexadecenoate with binding affinity of -7, -6.5, -5.9, -5.7 and -5.6 kcal/mol, respectively. It is very likely that these compounds can be candidates for therapeutic agents and these candidates need to be studied further.

}, doi = {10.5530/pj.2022.14.78}, author = {Priscilla Listiyani and Viol Dhea Kharisma and Arif Nur Muhammad Ansori and Muhammad Hermawan Widyananda and Rasyadan Taufiq Probojati and Ahmad Affan Ali Murtadlo and Dora Dayu Rahma Turista and Md. Emdad Ullah and Vikash Jakhmola and Rahadian Zainul} } @article {1873, title = {In Silico Screening of Bioactive Compounds from Garcinia mangostana L. Against SARS-CoV-2 via Tetra Inhibitors}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {575-579}, type = {Research Article}, chapter = {575}, abstract = {

The global COVID-19 pandemic caused by SARS-CoV-2 has been the resulted of massive human deaths since early 2020. The purpose of this study was to determine the potential of mangosteen (Garcinia mangostana L.) as an inhibitor of RBD spike, helicase, Mpro, and RdRp activity of SARS-CoV-2 with an in silico approach. The samples were obtained from PubChem and RCSB PDB. Analysis of the similarity of the drug was carried out with the Swiss ADME on the basis of Lipinski rule of five. Prediction of antivirus probabilities was carried out using PASS Online. Molecular screening was performed using PyRx through molecular docking. Discovery Studio was used for visualization. The bioactive compounds with the highest antiviral potential were indicated with the lowest binding affinity to the targeted proteins RBD spike, helicase, Mpro, and RdRp of SARS-CoV-2. The results indicated that mangiferin has the greatest potential as a potential antiviral. However, more research is required to validate the results of these computational predictions.

}, keywords = {Antiviral agent, Garcinia mangostana L., in silico, SARS-CoV-2}, doi = {10.5530/pj.2022.14.138}, author = {Nur Sofiatul Aini and Viol Dhea Kharisma and Muhammad Hermawan Widyananda and Ahmad Affan Ali Murtadlo and Rasyadan Taufiq Probojati and Dora Dayu Rahma Turista and Muhammad Badrut Tamam and Vikash Jakhmola and Elsa Yuniarti and Saddam Al Aziz and Muhammad Raffi Ghifari and Muhammad Thoriq Albari and Riso Sari Mandeli and Muhammad Arya Ghifari and Devi Purnamasari and Budhi Oktavia and Amalia Putri Lubis and Fajriah Azra and Fadhilah Fitri and ANM Ansori and Maksim Rebezov and Rahadian Zainul} } @article {1832, title = {In Silico Screening of Bioactive Compounds from Syzygium cumini L. and Moringa oleifera L. Against SARS-CoV-2 via Tetra Inhibitors}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {267-272}, type = {Original Article }, chapter = {267}, abstract = {

The global pandemic of COVID-19 has caused disastrous consequences for both humans and the economy. The purpose of this study was to determine the potential of juwet (Syzygium cumini L.) and moringa (Moringa oleifera L.) as inhibitors of RBD spike, helicase, Mpro, and RdRp activity of SARS-CoV-2 with an in-silico approach. Samples were obtained from PubChem and RSCB PDB databases. The drug similarity analysis was determined using Swiss ADME and the Lipinski rule of five. Prediction of antivirus probabilities is carried out with PASS Online. Molecular screening is performed by molecular docking using PyRx. Visualization was used using PyMol and Discovery Studio. The bioactive compounds with the best antiviral potential had the lowest affinity bonds to the target proteins against RBD spike, helicase, Mpro, and RdRp of SARS-CoV-2. Results show that ellagic acid from java plum and myricetin from moringa have the best potential as potential antivirals. However, more research is required to validate the results of these computational predictions.

}, keywords = {Antiviral agent, in silico, Moringa Oleifera L, SARS-CoV-2, Syzygium cumini L.}, doi = {10.5530/pj.2022.14.95}, author = {Nur Sofiatul Aini and Viol Dhea Kharisma and Muhammad Hermawan Widyananda and Ahmad Affan Ali Murtadlo and Rasyadan Taufiq Probojati and Dora Dayu Rahma Turista and Muhammad Badrut Tamam and Vikash Jakhmola and Devni Prima Sari and Muhammad Thoriq Albari and Devi Pernamasari and Muhammad Arya Ghifari and Muhammad Raffi Ghifari and Riso Sari Mandeli and Muhardi and Budhi Oktavia and Trisna Kumala Sari and Titi Sriwahyuni and Putri Azhari and Mirella Fonda Maahury and ANM Ansori and Rahadian Zainul} } @article {1872, title = {In Silico Study of Entry Inhibitor from Moringa oleifera Bioactive Compounds against SARS-CoV-2 Infection}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {565-574}, type = {Research Article}, chapter = {565}, abstract = {

The aim of this study is to screen the content of bioactive compounds of Moringa oleifera and to identify its potential as an antiviral against COVID 19 through an entry inhibitor mechanism using bioinformatics tools. The sample was obtained from PubChem database. Amino acis sequences were obtained from the NCBI. Protein modeling is made through the SWISSMODEL site. The target proteins for this study were SARS-CoV-2 Mpro and RdRp. The protein-inhibitory interaction of the drug from M. oleifera bioactive compounds to SARS-CoV-2 was predicted by molecular docking with PyRx software. The result shows that M. oleifera was a potential antiviral candidate for SARS-CoV-2 with an entry inhibitor mechanism through a compound, especially quercetin. The RFMS value of both interactions between Mpro and quercetion and RdRp with quercetin were not higher than 1.05. This result still needed further research to prove this prediction.

}, keywords = {Active site, COVID-19, Moringa oleifera, Mpro, RdRp}, doi = {10.5530/pj.2022.14.137}, author = {Nala Mawaddani and Ekris Sutiyanti and Muhammad Hermawan Widyananda and Viol Dhea Kharisma and Dora Dayu Rahma Turista and Muhammad Badrut Tamam and Vikash Jakhmola and Syamsurizal and Bayu Ramadhani Fajri and Muhammad Raffi Ghifari and Muhammad Thoriq Albari and Muhammad Arya Ghifari and Amalia Putri Lubis and Dony Novaliendry and Dwi Hilda Putri and Fadhilah Fitri and Devni Prima Sari and Alexander Patera Nugraha and ANM Ansori and Maksim Rebezov and Rahadian Zainul} } @article {1852, title = {Indonesian Medicinal Plants with Anti-inflammatory Properties and Potency as Chronic Obstructive Pulmonary Disease (COPD) Herbal Medicine}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {432-444}, type = {Review Article}, chapter = {432}, abstract = {

Indonesia is a tropical country with mega-biodiversity. Several medicinal plants locally have been recognized for their anti-inflammatory properties and are traditionally used to help treat respiratory diseases. Chronic obstructive pulmonary disease (COPD) is one of the diseases known as the high cause of death globally, and one of the treatment efforts is by using anti-inflammatory drugs. In developing alternative remedies for COPD, this review summarizes the potential of Indonesian medicinal plants and their ingredients known to have an anti-inflammatory activity to develop alternative remedies for COPD. Primarily, we focus on the medicinal plants that have been scientifically proven to pose some biological activities, such as legetan warak (Adenostemma lavenia), celery (Apium graveolens), pegagan (Centella asiatica), kenikir (Cosmos caudatus), and kersen (Muntingia calabura). This review is expected to provide more information about Indonesian medicinal plants and their potencies to be developed as COPD herbal medicine and, further, as a treatment to help patients suffering from coronavirus disease (COVID-19).

}, keywords = {Anti-inflammatory, Bioactive compounds, Biological activity, Chronic obstructive pulmonary disease, Indonesian medicinal plants}, doi = {10.5530/pj.2022.14.119}, author = {Min Rahminiwati and Trivadila and Dyah Iswantini and Hiroshi Takemori and Mamoru Koketsu and Rut Novalia Rahmawati Sianipar and Suminar Setiati Achmadi and Ahmad Sjahriza and Betty Marita Soebrata and Armi Wulanawati} } @article {1900, title = {Influence of Chitosan Concentration on Characteristic of Microspheres Delivery System Prepared from Eleutherine palmifolia (L.) Merr. Extract}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {702-709}, type = {Original Article}, chapter = {702}, abstract = {

Background: Eleutherine palmifolia (L.) Merr. (E.palmifolia) is a medicinal plant containing naphthoquinone, isoliquiritigenin, and oxyresveratrol compound. This study aims to formulate and characterize the E. palmifolia microspheres delivery system using chitosan variation as the polymer. Methods: The preparation method was the solvent evaporation method. The microspheres delivery system was performed by variation of chitosan concentration in each formulation 0.637\% (F1), 1.275\% (F2), and 1.912\% (F3). The physical and chemical characterizations include Entrapment Efficiency (EE), yield percentage, particle size, particle morphology, FTIR analysis, X-Ray Diffraction (XRD) analysis, and Differential Thermal Analysis (DTA) had been evaluated. Results: The results showed that chitosan concentration variation affected the microsphere{\textquoteright}s physical and chemical characteristics. Variations in the concentration of chitosan polymer (0.637\%, 1.275\% and 1.912\%) had an effect on the physical characteristics of the microspheres of the resulting Dayak onion bulb extract. The higher the concentration of chitosan used, the higher the entrapment efficiency and the yield yield will increase. Meanwhile, in observing the particle size, the higher the concentration of chitosan used, the smaller the particle size produced. The optimal concentration of chitosan as a polymer at concentration variations of 0.637\%, 1.275\% and 1.912\% to provide good physical characteristics of the Dayak onion bulb extract microspheres was shown in formula 3 with a chitosan concentration of 1.912\%. Conclusion: The better characteristic was obtained upon higher chitosan concentration. The best physical characteristics of E.palmifolia extract microspheres were obtained from chitosan concentration at 1.912\% (F3).

}, keywords = {Characteristic, Chitosan, Delivery system., Eleutherine palmifolia, Microspheres}, doi = {10.5530/pj.2022.14.157}, author = {Roihatul Mutiah and Wirda Ardania and Arief Suryadinata and Dewi Sinta Megawati and Anik Listiyana and Abdul Wafi and Rahmi Annisa} } @article {1736, title = {Liposome Preparation of Tekelan Leaf (Chromolaenaodorata L.) Extract: Manufacturing and Evaluation}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {February 2022}, pages = {56-62}, type = {Original Article}, chapter = {56}, abstract = {

Background: Liposomes are biocompatible, biodegradable, and non-immunogenic due to their phospholipid composition which is similar to cell membranes. Active chemicals that are contained in liposomes preparation has a goal in improving solubility, minimizing adverse effects, extending release, protecting drugs, targeting drugs, and increasing efficacy. Objective: The purpose of this study was to formulate the ethanol extract of tekelan leaves into liposome preparations and to determine differences in particle size, polydispersity index (PI) and stability. Materials and Methods: This research begins with making extract by maceration. The extract formed was then formulated into liposome preparations with the composition of F1 (30 mmol), F2 (40 mmol) and F3 (50 mmol) ingredients. The formulation was carried out by thin layer hydration with variations in sonication time of 10 minutes, 20 minutes and 30 minutes. Evaluation of liposomes was carried out organoleptically, testing pH, particle size, polydispersity index (PI), and physical stability at room temperature. Data analysis was carried out statistically using SPSS 20.0 One Way ANOVA method to see if there were differences in each group. The average pH test results of the three formulations were 6.2{\textpm}0.3. The particle size results in the 30th minutes obtained particle sizes of F1 (293.63{\textpm}1.6 nm), F2 (201.9{\textpm}1.5 nm) and F3 (143.17{\textpm}0.6 nm). Results: The results of the polydispersion index (PI) were F1 (0.307{\textpm}0.03), F2 (0.275{\textpm}0.06) and F3 (0.229{\textpm}0.02). The results of the observation of room temperature stability obtained that the organoleptic results formed two layers, namely a clear layer and a cloudy layer. In the One-Way ANOVA test, a significance value of p\>0.05 (0.068) was obtained. Conclusion: Liposomes produced from ethanol extract of tekelan leaves are stable when stored at low temperatures (4{\textpm}0.5oC) with an organoleptic state of odorless, dark green color and thick consistency.

Key words: Liposomes, Tekelan, pH, Stability, Particle size.

}, doi = {10.5530/pj.2022.14.8}, author = {S Rahmi and Rosidah and T Widyawati and Sumaiyah} } @article {1854, title = {Massive Pleural Effusion with Adenosine Deaminase (ADA) Test Positive and COVID-19 Confirmed: A Case Report}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {450-454}, type = {Case Report}, chapter = {450}, abstract = {

A 26-year-old man complained of shortness of breath for 3 days before the hospital admission. The patient had a history of coughing up blood and had consumed alcohol and drugs. Decreased vesicular auscultation and dull percussion in the left lateral pulmo. Laboratory result showed increased neutrophil-lymphocyte ratio C-reactive protein, D-dimer, procalcitonin, ferritin, and decreased albumin level. Pleural fluid analysis indicated the presence of exudate, SARS-CoV-2 PCR positive, and increased ADA level to 43 U/L. Based on the examination results, we suspected that the etiology of the massive pleural effusion was tuberculous pleurisy, particularly due to increased ADA levels. The patient was diagnosed with COVID-19 pneumonia with massive pleural effusion and tuberculous pleurisy. Massive pleural effusion in SARS-CoV-2 infection is rare. Thus, laboratory modalities for massive pleural effusion diagnosis are needed to determine the etiology and effective treatment for the patient. ADA analysis could be considered as an initial examination in patients with pleural effusion during the wait for pleural fluid culture results.

}, keywords = {ADA test, COVID-19, Infectious disease, Pleural effusion, Pneumonia, Tb pleuritis}, doi = {10.5530/pj.2022.14.120}, author = {Dewintha Airene Novianti and Puspa Wardhani} } @article {1929, title = {Molecular Docking Estrogen Receptor Alpha Antagonist and P53- MDM2 Inhibitor, ADMET Prediction of Alkaloid Compound from Mitragyna speciosa for Breast Cancer Therapy}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {912-916}, type = {Original Article }, chapter = {912}, abstract = {

Introduction: Breast cancer is one of the major universal health problems affecting more than two million cases per year. Estrogen receptor alpha (ERα) and P53 are common targets for the treatment of breast cancer and are primarily involved in cell proliferation. The function of p53 protein is regulated by direct binding to MDM2 protein. Therefore, inhibition of p53-MDM2 interaction leads to reactivating p53 activity. Alkaloid compounds generally have potential anticancer effect. Alkaloid compound from Mitragyna speciosa have the potential for anticancer. Methods: The method used is molecular docking with AutoDockTools 1.5.6 program. Predict the properties of physicochemical, pharmacokinetic, and toxicity prediction tests (ADMET) using pkCSM. Results: The results showed that speciophylline, corynoxine A, and corynoxine B have the best values in free binding energy (ΔG) for estrogen receptor (ERα) alpha receptor. Meanwhile, mitraphylline, mitrafoline, and corynoxine B have the best values for protein P53. Predict ADMET using the pkCSM, the alkaloid compound has strong lipophilicity and good permeability so it predicts the ability to penetrate intestinal cell membranes and the skin membrane. Spesiofilin, mitraphylline, and mitrafolin are not expected hepatotoxic. Conclusion: Speciophylline and mitraphylline have potential as anticancer drugs through the inhibitory of estrogen receptor alpha and MDM2 reseptor.

}, keywords = {ADMET, Alkaloid, Breast cancer, Docking, Mitragyna speciosa}, doi = {10.5530/pj.2022.14.188}, author = {Puja Adi Priatna and Rizki Rahmadi Pratama and Retno Widyowati and Sukardiman} } @article {1910, title = {Nano Transdermal Delivery Potential of Fucoidan from Sargassum sp. (Brown Algae) as Chemoprevention Agent for Breast Cancer Treatment}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {789-795}, type = {Research Article }, chapter = {789}, abstract = {

Conventional chemotherapy substances are associated with mild to severe side effects that affect both healthy and cancer cells. It is presumed to improve therapeutic efficacy in coexistence reducing chemotherapy{\textquoteright}s side effects. Fucoidan is an anticancer bioactive compound derived from Sargassum sp. that has low cytotoxic activity. The purpose of this study was to explore the effectiveness of anticancer activities of fucoidan from Sargassum sp. against breast cancer then analyze the suitability of nano transdermal patch of fucoidan and blueprint the long-term research design of nano transdermal patch as a chemoprevention agent in the chemotherapeutic management of breast cancer. This research was performed through a literature study and in silico study by imposing carbonic anhydrase IX (CA IX) as a marker of hypoxia and metastatic state of cancer cells. The results showed that the fucoidan from Sargassum sp. effectively induced apoptosis and prevented metastasis of breast cancer cells through the Bcl-2, Bcl-w, and bad pathways. Fucoidan, in addition, was predicted to inhibit CA IX by Glu4 Glu5, Leu7, Pro8, and Asp6 residues. Therefore, the delivery of fucoidan is favored to have a local effect on the site of breast cancer cells by nano transdermal patch preparations using fucoidan nanoparticle polymer. Further nano transdermal patch development as a treatment for breast cancer is suggested through the stages of formulation optimization, optimum formula activity testing, patent filing, and distribution in health services.

}, keywords = {Anticancer, Breast cancer, Fucoidan, Nano transdermal, Sargassum sp. .}, doi = {10.5530/pj.2022.14.169}, author = {Syeftyan Muhammad Ali Hamami and Michelle Fai and Ahmad Fariduddin Aththar and M Nizam Zulfi Zakaria and Viol Dhea Kharisma and Ahmad Affan Ali Murtadlo and Muhammad Badrut Tamam and Vikash Jakhmola and Muhammad Hermawan Widyananda and Dora Dayu Rahma Turista and Maksim Rebezov and Nikolai Maksimiuk and Nataliya Kulmakova and Evgeniya Latynina and ANM Ansori and Rahadian Zainul and Riso Sari Mandeli and Devi Purnamasari and Oski Illiandri and Khoirun Nisyak and Ernarisa Fitri} } @article {1892, title = {Parathyroid Carcinoma Mimicking Multiple Myeloma: A Tale of Refractory Hypercalcemia}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {863-866}, type = {Research Article }, chapter = {863}, abstract = {

Primary hyperparathyroidism yields various symptoms, including hypercalcemia, pathological fracture, and renal impairment. Parathyroid carcinoma is the rarest cause of primary hyperparathyroidism, accounting for \<1\% of the cases. We reported a case of a 46-year-old male with closed fractures at the humerus and femur. Further findings revealed severe refractory hypercalcemia, renal impairment, anemia, and bone lytic lesion (CRAB). No palpable cervical mass was identified. Surprisingly, the serum protein electrophoresis was normal, and urinary Bence-Jones protein was negative along with normal bone marrow aspiration. Hence, multiple myeloma was unlikely. The intact parathyroid hormone level was very high, along with a suspicious nodule on the left thyroid lobe (TIRADS 4). Total thyroidectomy and total parathyroidectomy were performed. The post-surgical pathological examination confirmed the diagnosis of parathyroid carcinoma. After the surgery, the patient was in stable condition with normal intact parathyroid hormone and serum calcium levels. In this case, primary hyperparathyroidism was caused by parathyroid carcinoma with {\textquotedblleft}CRAB{\textquotedblright} symptoms, mimicking multiple myeloma. Primary hyperparathyroidism should be considered in the patient with refractory hypercalcemia.

}, keywords = {Case report, Hypercalcemia, Multiple myeloma, Parathyroid tumor, Primary hyperparathyroidism}, doi = {10.5530/pj.2022.14.180}, author = {Christian Jonatan and Sony Wibisono} } @article {1874, title = {The phytochemical and pharmacological activity of extract Kirinyuh (Chromolaena odorata L.) leaves: A Review}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {580-586}, type = {Research Article}, chapter = {580}, abstract = {

C. odorata L. is considered to be a plant weed that is scattered in various climates. As a weed, this plant contains a variety of beneficial secondary metabolites. Several studies have shown the benefits of C. odorata L. leaf extract. This study reviews the metabolite content and the pharmacological activities of C. odorata L. leaf extract. A literature search was carried out to obtain various studies related to the use of this plant extract. Secondary metabolites identified in C. odorata L. are alkaloids, flavonoids, tannins, saponins, and steroids. Several reports have also shown that even though it is considered a weed, C. odorata L. leaf extract also provides many benefits due to its pharmacological activities. Various pharmacological activities include anti-inflammatory, anti-microbial, antioxidant, antidyslipidemia, hematologic agent, antidiabetic and anti-cataract, analgesic and antipyretic, wound healing, anti-malaria, mosquito larvicidal, antihypercholesterolemia, and antifungal.

}, keywords = {Characterization, Chromolaena odorata, Kirinyuh, Pharmacological activity, Phytochemical}, doi = {10.5530/pj.2022.14.139}, author = {Erna Harfiani and Yudhi Nugraha and Citra Ayu Aprilia and Feda Anisah Makkiyah and Ratna Puspita and Viol Dhea Kharisma and Muhammad Hermawan Widyananda and Ahmad Affan Ali Murtadlo and Dora Dayu Rahma Turista and Muhammad Badrut Tamam and Riso Sari Mandeli and Mirella Fonda Maahury and Devi Purnamasari and Muhammad Arya Ghifari and Muhammad Thoriq Albari and Muhammad Raffi Ghifari and Asmi Citra Malina A. R. Tasakka and Alexander Patera Nugraha and Rahadian Zainul} } @article {1781, title = {Potency of Antidiabetic Effects of the Combination of Syzygium cumini and Andrographis paniculata in Rats with High-Fat Dietand Streptozotocin-Induced Diabetes}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {April 2022}, pages = {406-412}, type = {Research Article }, chapter = {406}, abstract = {

Andrographis paniculata (AP) and Syzygium cumini (SC) are known for their antihyperglycemic effects. However, the combined effects of these plants have not yet been assessed. This study evaluated the oral acute toxicity and in vivo antihyperglycemic effects of the extract combining AP and SC (SCAP) in rats with high-fat diet- and streptozotocin (STZ)-induced diabetes. Thirteen female DDY mice for toxicity test were divided into three groups and orally administered one dose SCAP (0, 300, or 2000 mg/kg). On day 15, animals were euthanized, their internal organs were observed, and blood samples were collected for clinical biochemistry analyses. In vivo antihyperglycemic activity was examined in male Sprague- Dawley rats-induced diabetes. Diabetic rats were assigned to once-daily oral treatment with metformin, AP, SC or SCAP for 1 week. Concerning toxicity, SCAP had no effects on liver and kidney and histology of these organs displayed no abnormalities. Blood glucose levels had a tendency to reduce in treatment groups compared with the findings in the diabetic control group. SCAP treatment protected rats against pancreatic damage. These results illustrated that the combined SCAP treatment had beneficial effects on blood glucose levels and pancreatic β-cell function, in rats-induced diabetes.

}, keywords = {Andrographis paniculata, Combination, Diabetes, Syzygium cumini}, doi = {10.5530/pj.2022.14.52}, author = {Gumilar Adhi Nugroho and Febrika Wediasari and Zahra Fadhilah and Berna Elya and Heri Setiawan and ELFAHMI} } @article {1812, title = {Potential Roles of Purslane (Portulaca oleracea L.) as Antimetabolic Syndrome: A Review}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {710-714}, type = {Review Article}, chapter = {710}, abstract = {

The number of cases of obesity and type 2 diabetes mellitus (T2DM) is part of the metabolic syndrome case. Purslane (Portulaca oleracea L.) is a plant that has been clinically tested and has the potential to prevent and treat metabolic syndrome as well as pathogenic and pathophysiological activities that cause disease. The aim of this study is to discuss and conclude information regarding the activity and use of purslane (P. oleracea) as an antimetabolic. This review article is based on scientific publications found on Google Scholar and PubMed databases using the keywords of {\textquotedblleft}Portulaca obesity{\textquotedblright}, {\textquotedblleft}Portulaca overweight{\textquotedblright}, {\textquotedblleft}Portulaca dyslipidemia{\textquotedblright}, and {\textquotedblleft}Portulaca metabolic syndrome{\textquotedblright}. This plant acts on numerous pathways in the metabolic syndrome such as reduction of lipids, blood sugar, body weight and total cholesterol. Purslane (P. oleracea) can be used as a candidate for a new herbal plant as an anti-metabolic syndrome.

}, keywords = {Body weight, Insulin resistance, Metabolic syndrome, Obesity., Portulaca oleracea}, doi = {10.5530/pj.2022.14.90}, author = {Nur Sofiatul Aini and Arif Nur Muhammad Ansori and Viol Dhea Kharisma and Muhammad Farraz Syadzha and Muhammad Hermawan Widyananda and Ahmad Affan Ali Murtadlo and Rasyadan Taufiq Probojati and Md. Emdad Ullah and Sin War Naw and Vikash Jakhmola and Rahadian Zainul} } @article {1915, title = {Prostate Cancer: Causes and Medicinal Plants Used in Africa for Twenty Years (2001-2021)}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {822-828}, type = {Research Article }, chapter = {822}, abstract = {

Prostate cancer is one of deadliest cancers in the universe especially in Africa. A lot of work has been done on the treatment and control of breast cancer, colon/colorectal cancer, skin cancer, etc. Only a few works on prostate cancer has been published in literature. The conventional method of treatment used is almost not affordable by many cancer patients due to high cost of drugs and therapy. Also, the cumulative side effects arising from the treatment is enormous to patients. Hence, there is need to exploit local herbs that contain active anticancer ingredients that are readily available and affordable with little or no side effects. The present review was carried out to evaluate published works on medicinal plant species used for treatment and control of prostate cancer in Africa for twenty years (2001-2021). A total number of 48 plant species with 40 families were retrieved from database using ScienceDirect, Google scholar, Web of Science, Springerlink, Scopus, PubMed, and BioMed. All the plant species were reported to possess cytotoxic activity against prostate cancer cell lines in vitro and in vivo. The most cited plant species according to literature sources are Hypoxis hemerocallidea, Plumbago zeylanica, Gongronema latifolium and Mangifera indica. Plant families that were used more in treating prostate cancer were Apocynaceae, Fabaceae, Asteraceae, Cucurbitaceae, Bignoniaceae, Lamiaceae. We conclude that more research be carried out on the medicinal plants{\textquoteright} usage on the treatment of prostate cancer and further bio-prospecting drugs from the enlisted plants should explored by the pharmaceuticals in Africa.

}, keywords = {Cure, Death, Ethnobotany, Families, Phytomedicine, Tumour}, doi = {10.5530/pj.2022.14.174}, author = {Oludare Oladipo Agboola and Samuel Bawa Ijimbili and Gift Onyinyechukwu Ofuasia and Joseph Anejo-Okopi and Jasini Alexander Wahedi and Joshua Oluwole Olowoyo} } @article {1847, title = {Radiographic and Histological Evaluation in Canine Femur after Implantation of 304 Stainless-steel-based Plate}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {388-392}, type = {Research Article }, chapter = {388}, abstract = {

Fractures are known to be high-risk traumatic cases in domestic animals. Surgery was performed to reposition and immobilize bone using a plate as a biomaterial component. This study aimed to evaluate the radiographic and histological findings in canine femur after implantation of a 304 stainless-steel-based plate. A total of six male dogs aged 3-4 months were used in this study. Dogs were acclimatized for a week and then randomly assigned to 2 groups and 3 replication, respectively. The left femoral diaphysis was cracked and fixed by (A) commercial plate; (B) 304 stainless-steel plate. Radiographic observations were performed at 24 h and 28 d postoperatively during the recovery period. Histological figures were evaluated at 28 d postoperative descriptively. As a result, physical examination of muscle tissue in both groups did not reveal discoloration, swelling, and fluid accumulation around the plate. The radiographic figures presented a slight callus production around the crack and a decrease in the gap between cracks after 28 d. The histological figures observed the proliferation of osteoblasts, osteocytes, connective tissues, and bone trabeculae. It can be concluded that no significant difference between 304 stainlesssteel plate compared to commercial plate. A 304 stainless-steel-based plate is recommended for bone immobilization in canine femoral fractures.

}, keywords = {304 stainless-steel, Domesticated animal, Femur, Fracture, Radiograph}, doi = {10.5530/pj.2022.14.112}, author = {Agus Purnomo and Hartiningsih and Setyo Budhi and Dhirgo Adji and Devita Anggraeni and Dito Anggoro and Sitarina Widyarini and Shekhar Chhetri and Muhammad Thohawi Elziyad Purnama} } @article {1932, title = {Relationship Histopathology Grading of Meningioma with the Use of Medroxyprogesterone Acetate (MPA) as A Hormonal Contraceptive}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {938-941}, type = {Original Article }, chapter = {938}, abstract = {

Introduction: Meningioma is a common brain tumor with an incidence of more than 30\% of all primary brain tumors in adults. The incidence of meningiomas increases with increasing age, women suffer more from meningiomas with a ratio of 3:1. Meningiomas are known to have steroid receptors in the form of progesterone (88\%), estrogen (40\%), and androgen (40\%). Therefore, the use of hormonal therapy is suspected to affect the incidence and histopathological degree of meningioma. One of the widely used hormonal therapy is medroxyprogesterone acetate (MPA). However, there is not enough literature to explain the relationship between MPA and the incidence of meningioma. Objective: To identify the relationship between the duration of the use of Medroxyprogesterone acetate (MPA) hormonal contraceptives and the histopathological degree of meningioma. Methods: This study is an analytic observational with a retrospective design of meningiomas patients based on the duration of use of MPAtype hormonal contraception who performed surgery at Dr. Soetomo General Academic Hospital during the period January 2015 to December 2019. We determined the inclusion criteria for meningioma patients: a history of using MPA hormonal contraceptives or 3-month injectable contraceptives; and the control group: meningioma patients without a history of hormonal contraception. Results: There were 452 cases of meningioma and 101 patients met the inclusion criteria. Based on the results of statistical analysis, it was found that there was no difference relationship between the grade of meningioma and meningioma patients either using MPA or without using MPA. There was no difference in duration of use between the group using MPA \<10 years or more than 10 years with the non-hormonal control group on meningioma grading (p = 0.772). There was also no difference relationship between the group that did not use hormonal contraception and the MPA group on the age of the patient (p = 0.217), both using contraception for \<10 years and more than 10 years. Conclusion: There was no relationship found between histopathological degree of meningioma with the patients who use MPA contraceptives, both duration of use \<10 years and >=10 years compared with meningioma patients who do not use MPA contraception.

}, keywords = {Contraception, Grading, Hormonal contraceptive, Medroxyprogesterone acetate, Meningioma, MPA, Neoplasm., Progesterone}, doi = {10.5530/pj.2022.14.193}, author = {Shafhan Dustur and Joni Wahyuhadi and Budi Utomo and Muhammad Arifin Parenrengi and Abdul Hafid Bajamal and Sri Ratna Dwiningsih} } @article {1787, title = {A Review of an Important Plants: Annona squamosa Leaf}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {April 2022}, pages = {456-463}, type = {Review Article}, chapter = {456}, abstract = {

Plants have been utilized extensively in traditional medicine by a range of nations since ancient times, and more research into their safety, efficacy, and quality value is needed. One of the plants that have been widely used by society in traditional medicine is Annona squamosa L. A. squamosa is commonly cultivated in tropical and subtropical regions. Based on previous research, all parts of A. squamosa including bark, leaf, and roots have proven biological activities such as antioxidant, antifungal, and anticancer, especially on the leaves. Indian people have long history used young leaves of A. squamosa for antidiabetic, besides in South China, they use seeds to decrease the cancer effect in the human body. The pharmacological activities of A. squamosa leaves are antimicrobial, antifungal, anti-inflammatory, anticancer, antiulcer, antidiabetic, antidiarrheals, antiplatelet, antioxidant, and hepatoprotective, neuroprotective, and cytoprotective. Phytochemicals in A. squamosa leaves include coumarins, tannins, cardiac glycosides, flavonoids, carbohydrates, and saponins. Meanwhile based on nutritional analysis shows that the A. squamosa leaves are water, protein, lipids, Fiber Ash and Calcium.

}, keywords = {Annona squamosa, Medicine, Pharmacology, Phytochemistry, Traditional medicinal plant}, doi = {10.5530/pj.2022.14.58}, author = {Arifia Safira and Prasita Widayani and Dhiya An-Najaaty and Cinta Atsa Mahesa Rani and Mela Septiani and Yan Arengga Syah Putra and Tridiganita Intan Solikhah and Aswin Rafif Khairullah and Hartanto Mulyo Raharjo} } @article {1811, title = {Role of Alkaloid on Platelet Aggregation and Serotonin in Migraine}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {629-632}, type = {Research Article}, chapter = {629}, abstract = {

Migraine is a sterile neurogenic inflammation of the trigeminal nerve which releases vasoactive neuropeptides and activates platelets to release vasoactive substances such as serotonin (5-HT). Platelet hyperaggregation occurs in the pathogenesis of migraine caused by one of the stimulatory factors 5-HT. Platelet aggregation is increased and 5-HT levels are elevated in the blood and brain in the early stages of migraine. Alkaloid β-carbolin alkaloids can increase monoamines in brain regions through inhibition of monoamine oxidase (MAO) and inhibition of 5-HT reuptake. Alkaloids in the ethanolic extract of SCE function as analgesics and anti-inflammatory which can reduce pain and improve blood circulation. Sargassum cristaefolium extract (SCE) was measured for its bioactive substance content. The extract was administered to an animal model of intraperitoneal nitroglycerin-induced migraine and examined for platelet levels, platelet aggregation and 5-HT. The results of statistical tests showed an increase in platelets (p\<0.05), an increase in platelet aggregation (p\<0.05) and a decrease in 5-HT (p\<0.05). The relationship between alkaloids and platelets; platelets and platelet aggregation; platelet aggregation and 5-HT and 5-HT levels and migraine incidence (p\<0.05). The alkaloids found in SCE can lower platelet count, decrease platelet aggregation and increase 5-HT levels in migraines.

}, keywords = {Alkaloid, Migraine, Platelet, Platelet aggregation, Serotonin.}, doi = {10.5530/pj.2022.14.81}, author = {Olivia Mahardani Adam and Widjiati Widjiati} } @article {1950, title = {The Role of Breast Milk on Reducing the Risk of Neonatal Sepsis in Preterm and Low Birth Weight Infants: A Systematic Review and Meta-Analysis}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {1067-1074}, type = {Review Article}, chapter = {1067}, abstract = {

Background: High mortality and morbidity rates are associated with neonatal sepsis in preterm and low birth weight infants. Aside from controlling the nosocomial infection, intervention for reducing the risk of sepsis is demanded. The best nutrition for preterm infants is breast milk. Bioactive compounds found in it, such as antibacterial, antiviral, and anti-inflammatory activities not only for immunity against the infection but also for growth, and development. Objective: To investigate the effect of breast milk against the risk of neonatal sepsis in preterm and low birth weight infants. Methods: We conducted an electronic search through several databases including PubMed, Web of Science, Science Direct, and Scopus. We performed an analysis on nutritional feeding and volume of breast milk and late-onset sepsis from ten potential observational studies. Results: Breast milk significantly reduced the risk of sepsis in preterm and low birth weight infants (pooled RR 0.70; 95 \% CI 0.55 - 0.88, p = 0.002). In addition, when we performed subgroup analysis, we found that breast milk volume \> 50ml/kgbw/day also reduce the risk of sepsis with pooled RR 0.61(95\% CI 0.46-0.8, p=0.0004). Conclusion: Low birth weight and preterm infants had a lower risk of neonatal sepsis when they got breastmilk. To preserve the supply of breastmilk, health professionals should support and encourage mothers who were breastfeeding.

}, keywords = {Breast milk, Low birth weight, Neonatal sepsis, Preterm infant}, doi = {10.5530/pj.2022.14.211}, author = {Tita Natalia Manurung and Citrawati Dyah Kencono Wungu and Martono Tri Utomo} } @article {1881, title = {Study of Sericin Sequences from Bombyx mori as Antiaging through ROS with Molecular Simulation and DPPH Evaluation}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {632-641}, type = {Research Article}, chapter = {632}, abstract = {

The presence of ROS is associated with aging, which is damage caused by free radical reactions. ROS causes oxidation of low density lipoprotein (LDL), which builds up in plaque and contributes to inflammation. With aldehyde secondary products of lipid peroxidation such as Malondialdehyde (MDA), lipoxygenase, and xanthine oxidase as markers of oxidative stress, oxidized LDL causes endothelial dysfunction and cell apoptosis. The antioxidant 1,1 diphenyl-2-picrylhydrazyl (DPPH) sericin from Bombyx mori was tested in silico and in vitro in this study. The Bombyx mori peptide sequences QAYADYHSDPNGGSA (SP4) and ASSSFDASSA (SP7) had lower Gibbs energy for lipooxygenase (LOX) than native ligands, with values of -23.1044, -21.0056, and -10.3275 kcal/mol, respectively. hydrogen bonding to Gln289, Asp293, and Gly569. While ASSSFDASSA (SP7) has a higher Gibbs energy for xanthine oxidase (XOX), SEASSSTQATTVS (SP 5) has a lower Gibbs energy with values of -20.1839, -17.8952, and -11.8921 kcal/mol, respectively. While the cavity binding of the xanthine oxidase peptide binding SP5 and SP7 is located at the Glu802, Asp872, and Ser876 binding sites, the DPPH test confirmed in vitro that the 10\% sericin Gel had an IC50 of 19.7394 ppm compared to 3.71 ppm ascorbic acid. The findings of the preceding study demonstrate that sericin, as an antioxidant, is one of the candidates for antiaging.

}, keywords = {DPPH., LOX, ROS, Sericin}, doi = {10.5530/pj.2022.14.146}, author = {Fitria Agustina and Fadilah Fadilah and Wimpie Pangkahila and Anak Agung Gde Putra Wiraguna and I Gusti Ayu Sri Mahendra Dewi} } @article {1853, title = {Thalassemia β Major in Confirmed Covid-19 Patient: A Case Report}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {445-449}, type = {Case Report}, chapter = {445}, abstract = {

The incidence of Thalassemia with confirmed Covid 19 is very rare. The aim of this study is to know the prognosis and evaluate the management of therapy in thalassemia β Mayor patients with COVID-19. This case study describes the progression of Thalassemia β Mayor with COVID 19 starting from how to establish the diagnosis of Thalassemia β Mayor and COVID 19 until how to treat thalassemia β Mayor patients with COVID-19 This case study result describes that COVID 19 aggravates thalassemia β Mayor. Infection can adversely affect thalassemia. Giving Blood transfusions must be done due to anemia in Thalassemia, but it will have an impact on the accumulation of iron in the body which will increase the severity of the infection. The administration of iron-chelation drugs is beneficial for Covid but on the other hand, it is contraindicated in Thalassemia patients.

}, keywords = {COVID-19, Infection, Preventable Death, Thalassemia}, doi = {10.5530/pj.2022.14.119}, author = {Yosua Butar Butar and Puspa Wardhani} } @article {1818, title = {Total Polyphenols, Total Flavonoids, Antioxidant Activity and Inhibition of Tyrosinase Enzymes from Extract and Fraction of Passiflora ligularis Juss}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {672-680}, type = {Research Article}, chapter = {672}, abstract = {

Background: Sweet granadilla (Passiflora ligularis Juss) grows in the cool highlands of Indonesia, one of which is the province of West Sumatera. Sweet granadilla has potent antioxidant activity and can inhibit the tyrosinase enzyme. Objective: This study was performed to determine content of total polyphenols, total flavonoids, antioxidant activity, tyrosinase inhibition in different part of P. ligularis extract and fraction. Materials and Methods: Leaves, stems, peels and seeds P. ligularis were separately extracted by the ultrasound-assisted extraction (UAE) method using 70\% ethanol. Then, the ethanol extract was fractionated using n-hexane, ethyl acetate and distilled water. The ethanol extract and active fraction were determining antioxidant activity using FRAP and DPPH method, inhibition of tyrosinase enzyme, total polyphenol and total flavonoid content. This study was equipped with analysis of light microscopy, SEM microscopy and LC-MS. Results: The highest total polyphenol content was found in the seed extract 176.22 {\textpm} 1.51 mg GAE/g extract and total flavonoid content was found in leaves extract 5.77 {\textpm} 0.48 mg QE/g extract. The highest antioxidant activity by FRAP method was found in seeds extract 80.79 {\textpm} 1.29 g Fe2SO4 equivalent/100 g extract and DPPH method was found in stem extract with IC50 value 9.00 {\textpm} 0.09. The highest percentage of tyrosinase inhibition (1 mg/ml) was found in seed extract 52.4 {\textpm} 2,55 \%. In fraction of seed extract show that ethyl acetate fraction most active than others. Conclusion: These results indicate that ethyl acetate fraction of seed P. ligularis has potent antioxidants and good inhibition of the tyrosinase enzyme.

}, keywords = {Antioxidant, Passiflora ligularis Juss, Sweet granadilla., Tyrosinase, Ultrasound-assisted extraction (UAE)}, doi = {10.5530/pj.2022.14.86}, author = {Selvia Wiliantari and Raditya Iswandana and Berna Elya} } @article {1800, title = {Utilization of Secondary Metabolites in Algae Kappaphycus alvarezii as a Breast Cancer Drug with a Computational Method}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {536-543}, type = {Original Article}, chapter = {536}, abstract = {

Breast cancer is one of the worst diseases that affect female people. Long-term treatment with therapy or surgery has a detrimental impact on the patient. The algae Kappaphycus alvarezii has gotten a lot of interest as a breast cancer medication because it contains chemicals that are expected to be anti-cancer. The objectives of this paper were to see how secondary metabolites in algae interact with the Nuclear Factor- kappaB protein kinase in breast cancer. The ligands and proteins were obtained from the PubChem and PDB websites, respectively. Swiss ADME was then used to assess the Pharmacokinetics and Drug likeness Properties. The last stage involved using molecular docking with PyRx and molecular dynamics to identify the interaction and visualization between the ligand and the target protein. The findings of the test revealed that the maraniol chemical had a superior binding capacity with NF kB protein kinase because it has a chromone group that controls transport efficiently in preventing breast cancer proliferation.

}, keywords = {Breast cancer, K. alvarezii, Molecular Docking., NF kB protein kinase}, doi = {10.5530/pj.2022.14.68}, author = {AF Dibha and S Wahyuningsih and ANM Ansori and VD Kharisma and MH Widyananda and AA Parikesit and MT Sibero and RT Probojati and AAA Murtadlo and JP Trinugroho and TH Sucipto and DDR Turista and I Rosadi and ME Ullah and V Jakhmola and R Zainul} } @article {1887, title = {Vancomycin, Linezolid, and Ceftaroline In vitro Activity Against Methicillin susceptible Staphylococcus aureus (MSSA) and Methicillin-resistant Staphylococcus aureus (MRSA) Isolates}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {671-674}, type = {Research Article}, chapter = {671}, abstract = {

MRSA-infected patients incur twice as many costs as MSSA-infected patients. Vancomycin, Linezolid, and, most recently, Ceftaroline are among Indonesia{\textquoteright}s several MRSA treatment options. Therefore, we sought to assess the efficacy of these three medications. The investigation was done at the Dr. Soetomo General Academy Hospital{\textquoteright}s Clinical Microbiology Laboratory in Surabaya. The bacterium ATCC 25923, ATCC 43300, MSSA clinical isolate, and MRSA clinical isolate of Staphylococcus aureus were studied. Vancomycin, Linezolid, and ceftaroline were administered at respective dosages of 1 MIC, 2 MIC, and 4 MIC. In addition, a time-kill test was performed, which consisted of counting the growth of colonies on solid media, generating a time-kill curve, and determining MBC. The number of colonies in the antibiotic groups at 4, 6, and 8 hours varied significantly, according to the study (Vancomycin, Linezolid, and Ceftaroline). In contrast, the number of bacteria did not differ significantly between Vancomycin and Linezolid until the fourth hour. Except at 6 and 24 hours, neither Vancomycin nor Ceftaroline significantly altered the number of bacteria. There was a significant difference in the number of colonies between Ceftaroline and Linezolid at 4, 6, and 8 hours. Vancomycin, Linezolid, and Ceftaroline against MSSA and MRSA isolates vary greatly.

}, keywords = {MRSA, MSSA, Time-kill curve}, doi = {10.5530/pj.2022.14.152}, author = {Eny Purwoningsih and Pepy Dwi Endraswari and Agung Dwi Wahyu Widodo} } @article {1697, title = {Analyzing of Urine 3-Hidroxy Propil Mercapturic Acid on Cyclophosphamide Induced Rat to Determine Ameliorating Effect of Leucaena leucocephala (Lam.) de Wit Seed Extract}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {December 2021}, pages = {1549-1556}, type = {Original Article}, chapter = {1549}, abstract = {

Background: A 70\% ethanolic extract of Leucaena leucocephala (Lam.) de Wit seeds contain a certain amount of sulfhydryl active compounds and potential for ameliorated cyclophosphamide side effects. Objective: The aim of this study was to analyze of urine 3-hidroxy propil mercapturic acid (3-HPMA) in a rat model for cyclophosphamide-induced hemorrhagic cystitis to determine the effect of Leucaena leucocephala (Lam.) de Wit seeds extract. Materials and methods: The levels of urine 3-hidroxy propil mercapturic acid was measured using LCMS/MS. Urine preparation was carried out by dilution (1:5) with mobile phase and contained a deuteurated internal standard. The LC separation was performed using a C18 column (1.7 μm; 2.1 mm {\texttimes} 100 mm). The mobile phase consisted of formic acid (0.1\%) and formic acid (0.1\%) in acetonitrile (90:10 v/v), as a starting gradient at flow rate of 0.2 ml/min with total run 7 min. Results: The lower limit of quantification was 40 ng/ml and the linear extended to 20,000 ng/ml. The method has been successfully to be applied in determined of rat urine 3-HPMA. The results showed that the 70\% ethanolic extract of seeds can increased the amount of 3-HPMA in rat urine. Conclusion: The 70\% ethanolic extract of seeds displayed a potential ameliorating effect against cyclophosphamide side effects.

}, keywords = {3-Hidroxypropil mercapturic acid, Cyclophosphamide, Leucaena leucocephala (Lam.) de Wit, Urine.}, doi = {10.5530/pj.2021.13.197}, author = {Sri Wardatun and Yahdiana Harahap and Noorwati Sutandyo and Abdul Mun{\textquoteright}im} } @article {1404, title = {The Anthelmintic Activity of Cawat Anuman (Bauhinia Sp.) Leaves Against Ascaridia galli Worms}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {626-630}, type = {Original Article}, chapter = {626}, abstract = {

Background: Traditionally, the people in Anik Hamlet usually use the Bauhinia sp. leaves as anthelimintic. Objective: The research was conducted to observe the anthelmintic activity of the ethanol extract of cawat anuman (Bauhinia sp.) leaves against Ascaridia galli by in vitro procedure. Methods: Group I was treated with NaCl 0.9\% as the negative control, also group II and III were treated with cawat anuman leaves ethanol extract at dose of 25 mg/ml and 50 mg/ ml respectively, and group IV was treated with mebendazole at dose 5 mg/ml as the positive control. Results: Compared to the negative control, the ethanol extract of cawat anuman leaves has activity as an anthelmintic (p\<0.05), moreover the potency of leaves extract at dose 50 mg/ml as anthelmintic against Ascaridia galli as strong as mebendazole at dose 5 mg/ml (p\>0.05). Conclusion: It concluded that the ethanol extract of cawat anuman leaves at dose 50 mg/ml has anthelmintic potency as strong as mebendazole 5 mg/ml.

}, keywords = {Anthelmintic activity, Ascaridia galli, Bauhinia sp. Leaves}, doi = {10.5530/pj.2021.13.79}, author = {Ruqiah Ganda Putri Panjaitan and Ela Elisa and Eko Sri Wahyuni} } @article {1690, title = {Antidiabetic Activity and Phytochemical Constituents of Syzygium cumini Leave in Kadipaten, Central Java Indonesia, Indonesia}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1502-1508}, type = {Research Article}, chapter = {1502}, abstract = {

Introduction: Ethnomedicaly, jamblang (java plum) has been used as antidiabetic treatment in Dayak Tribe. This study aims to evaluate the effectiveness and the safety of the jamblang leaf extract as an antidiabetic. Objective: Evaluate Effecticity of Syzigium cumini (java plum) as anti-diabetic herb. Method: The antidiabetic activity test, used an animal model which gaven food a high fat diet High Fat Diet (HFD) then it was induced with Streptozotocin injected intraperitoneally. The subjects used in the study were male rats (Ratus novergicus) Sprague-Dawley strain. Results: Oral administration of jamblang extract has anti hyperglycemic activity through decrease of fasting blood glucose point significantly (dose 1, 50mg/ Kg Bw p: \<0.0001, dose 2, 100 mg/Kg BW p: \<0.0001).

}, keywords = {Anti-hyperglicemic, Fasting blood glucose, Syzigium cumini}, doi = {10.5530/pj.2021.13.191}, author = {Zahra Fadhilah and Berna Elya and Heri Setiawan and Gumilar Adhi Nugroho and Febrika Wediasari and Eem Masaenah and Varda Arianti} } @article {1667, title = {Anti-Inflammatory Effects of Methanol Extract, Hexane, Ethyl Acetate, and Butanol Fraction of Piper crocatum Ruiz \& Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1341-1346}, type = {Original Article}, chapter = {1341}, abstract = {

Piper crocatum Ruiz \& Pav is a traditional Indonesian plant that is empirically used to treat various diseases. Several studies have stated that Piper crocatum leaves extract has anti-inflammatory effects. Piper crocatum leaves contain different secondary metabolites alkaloids, flavonoids, tannin-polyphenols, steroids, terpenoids, and saponins. The purpose of this study was to determine the anti-inflammatory effect of Piper crocatum leaves extract and fraction on the production of nitric oxide in lipopolysaccharideinduced RAW 264.7 cells. Anti-inflammatory effect of methanol extract of Piper crocatum leaves (MEPC), n-hexane fraction of Piper crocatum leaves (HFPC), ethyl acetate fraction of Piper crocatum leaves (EAFPC), and n-butanol fraction of Piper crocatum leaves (BFPC) against RAW 264 cell macrophages, 7 Lipopolysaccharide-induced (LPS). Extraction of Piper crocatum leaves was the maceration method. Cell viability was determined by the MTT method in the concentration range of 12.5;25;50;100;200 ug/ mL. The anti-inflammatory effects of MEPC, HFPC, EAFPC and BFPC were tested against nitric oxide (NO) production inhibition in LPS-induced RAW 264.7 macrophage cells. NO levels were determined by the NO Colorimetric Assay Kit as measured by the ELISA plate reader. The viability test of MEPC, HFPC, EAFPC, and BFPC from Piper crocatum leaves showed the concentrations that gave the percentage of viability above 80\% were concentrations of 100, 50, and 25 g/mL. MEPC, HFPC, EAFPC, and BFPC at concentrations of 100, 50, 25 μg/mL could significantly inhibit NO production with p\<0.05 (p=0.000). The highest NO production inhibition effect was on EAFPC. MEPC, HFPC, EAFPC, and BFPC have antiinflammatory effects, with the highest effect on EAFPC.

}, keywords = {Anti-inflammatory Effect, LPS, Nitric oxide, Piper crocatum}, doi = {10.5530/pj.2021.13.169}, author = {Nilda Lely and Helmi Arifin and Yufri Aldi and Fatma Sri Wahyuni} } @article {1397, title = {The Anti-Malarial Effect of Thespesia populnea (L.) Soland ex Correa Extract Using Malaria Mice Model Infected with P. berghei}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {585-590}, type = {Research Article}, chapter = {585}, abstract = {

Introduction: Malaria is a re-emerging disease that still causes high morbidity and mortality rates. Reports of malaria therapy, encouraging studies to find new therapies based on plants and adjuvant in malaria infection. Thespesia populnea or portia tree leaf extract is a plant that has been shown to have anti-inflammatory, antibacterial and antifungal effects. The aim of this study was to analyze the effect of Thespesia populnea leaf extract as antimalarial levels, and and its effect on hemoglobin levels in BALB/C mice infected with Plasmodium berghei ANKA (PbA). Methods: The study was true experimental laboratories using post-test only group design. Using 27 mice were randomly divided into 3 groups: Group with aquades (G1), Group received Chloroquine (G2), and Group with 200 mg/kg bw of Thespesia populnea (L.) Soland ex Correa extract and Chloroquine (G3). The degree of parasitemia was observed serially from the first day to the fourth day. Observation of the number of leukocytes and hemoglobin on the fourth day after the mice were terminated. Results: The results of statistical analysis showed that the administration of Thespesia populnea leef extract 200 mg/kg bw with chloroquine had the effect of decreasing the degree of parasitemia and increasing hemoglobin significantly than G1 group, but there was no significant difference with G2 group, although descriptively there were differences. Conclusion: Based on the results of this study, it can be concluded that Thespesia populnea or portia tree leaf extract has an anti-malarial effect and can also significantly prevent anemia.

}, keywords = {BALB/C, Hemoglobin, Malaria, Parasitemia, Thespesia populnea extract}, doi = {10.5530/pj.2021.13.73}, author = {Prawesty Diah Utami and Herin Setianingsih and Indira Firdha Syafitri and Rico Pratama Wiyono} } @article {1422, title = {Antiviral Activity of an Extract from Leaves of the Tropical Plant Cynometra cauliflora}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {752-757}, type = {Research Article}, chapter = {752}, abstract = {

Background: Cynometra cauliflora is a species of tree in the family Fabaceae and has been used in folk medicinal preparation. Objectives: In this study, Cynometra cauliflora methanolic leaves extract was tested against clinical isolate herpes simplex virus type-1 (HSV-1). Materials and Methods: The leaves of C. cauliflora plant was extracted using methanol extraction method. Cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT) assay. Plaque reduction assays were carried out to evaluate the antiviral activity of C. cauliflora extract against HSV-1. These include post-treatment, pre-treatment and virucidal assays. Results: The value of cytotoxic concentration, CC50 of C. cauliflora extract was 36 mg/ mL. High antiviral activity was observed in post-treatment. C. cauliflora extract treatment was found to not interfere directly to infectious particle and confer mild protection when given as prophylaxis. Conclusion: This study provides important novel insights on the phytomedicinal properties of C. cauliflora extracts on HSV-1.

}, keywords = {(3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide); virucidal., Cynometra cauliflora, Herpes simplex virus type 1, plaque reduction assay}, doi = {10.5530/pj.2021.13.96}, author = {Noor Zarina Abd Wahab and Aziah Azizul and Norhidayah Badya and Nazlina Ibrahim} } @article {1634, title = {Chitosan-Tripolyphosphate Nanoparticles of Mango Ginger (Curcuma mangga) Extract: Phytochemical Screening, Formulation, Characterization, and Antioxidant Activity}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1065-1071}, type = {Original Article}, chapter = {1065}, abstract = {

Introduction: Mango ginger (Curcuma mangga) is one of Indonesia{\textquoteright}s medicinal plants widely used in most communities as a lust booster and for detoxifying purposes. Therefore, the purpose of this study is to synthesize chitosan-tripolyphosphate nanoparticles from mango ginger extract, determine their chemical contents, the nano chitosan characteristics, and its antioxidant activity. Methods: In this study, we macerated mango ginger using 70\% ethanol solvent, then performed phytochemical test and formulation of chitosan nanoparticles of mango ginger extract. The group of secondary metabolites that showed positive results with the reagent test was further identified through TLC. Results: The results showed that the extract contained flavonoids and triterpenoids. Also, characterization of chitosan nanoparticles from the extract was conducted with FTIR test, PSA, XRD, and SEM. Based on the results, the nano chitosan particle size was 993 nm and examination with FTIR showed the presence of N-H and P=O groups, indicating ammonium ion interaction from chitosan with the polyanion from TPP and Mango ginger. Additionally, the XRD results showed that the crystals formed were in an amorphous form, which was supported by particle morphology images from SEM. Furthermore, the nanoparticles showed very strong antioxidant activity based on the reaction with DPPH. Conclusion: Based on these results, the phytochemical identification of mango ginger extract showed positive results in flavonoid and triterpenoid compounds. In addition, based on the characterization of the nanoparticles, the mango ginger extract showed positive results, illustrating that the nano chitosan synthesis was successful. Furthermore, the nano chitosan has a very strong antioxidant activity with an IC50 value of 18.08 μg/mL.

}, keywords = {Chemical identification, Chitosan, Mango ginger, Nanoparticles, TPP}, doi = {10.5530/pj.2021.13.138}, author = {Bayyinatul Muchtaromah and Didik Wahyudi and Mujahidin Ahmad and Arif Nur Muhammad Ansori and Rahmi Annisa and Lil Hanifah} } @article {1370, title = {Cytotoxicity Study of Ethanol Extract of Bintangor Leaf (Calophyllum soulattri Burm.f) on T47D Breast Cancer Cell Line (Cytotoxicity Study with MTT Assay Method)}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {362-367}, type = {Original Article}, chapter = {362}, abstract = {

Introduction: The public has used Bintangor leaf (Calophyllum soulattri Burm.f) for various medical treatments, including treated inflamed eyes and gout. Aim: This research aimed to determine the cytotoxic effect of ethanol extract and fraction of Calophyllum soulattri Burm. f leaf toward T47D breast cancer cell. Methods: The test used T47D breast cancer cells, the 3-4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium bromide (MTT) test method, and ELISA Reader to determine the absorbance. This method{\textquoteright}s principle was the presence of tetrazolium salts by the reductase system in the mitochondria of living cells formed purple formazan crystals. The used parameter was the value of IC50. Results: The result showed that ethanol extract, n-hexane fraction, ethyl acetate fraction, and butanol fraction did not have a cytotoxic effect on T47D breast cancer cell. The values of IC50 respectively are 585.31 μg/ml; 409.33 μg/ ml; 534.08 μg/ml; and 563.22 μg/ml. Conclusion: Ethanol extract and Calophyllum soulattri Burm.f leaf fraction did not have a cytotoxic effect on T47D breast cancer cells.

}, keywords = {Bintangor Leaf, Breast Cancer Line, Calophyllum soulattri Burm.f, Cytotoxicity, MTT Assay, T47D}, doi = {10.5530/pj.2021.13.46}, author = {Elidahanum Husni and Fatma Sri Wahyuni and Hanifa Nurul Fitri and Elsa Badriyya} } @article {1689, title = {The Cytotoxicity Study of Lantana camara Linn Essential Oil on HeLa Cancer Cells Line}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1498-1501}, type = {Research Article}, chapter = {1498}, abstract = {

Lantana camara Linn (Verbenaceae) is a natural plant that thrives in tropical climates and is relatively easy to cultivate. In Indonesia, this plant is still often considered as a weed. When held, the unpleasant smell and sticky hand make people dislike this plant even though the flowers are diverse. The essential oil was extracted from the leaves of L. camara by hydrodistillation. This study aimed to see how cytotoxic L. camara essential oil was against HeLa carcinoma cells. This research aimed to discover if L. camara essential oil was cytotoxic to HeLa cancer cells. The GC-MS investigation of an essential oil recognized ten compounds; two main constituents of the oil were Caryophyllene (27.65\%) and Germacrene D (23.01\%). The essential oil showed cytotoxicity on HeLa cervical cancer cell lines. The cytotoxic effect of oil was determined using MTT, IC50 values were 44.86 μg/mL + 0.07

}, keywords = {Cervical cancer, Cytotoxicity, HeLa, Hydrodistillation, Lantana camara}, doi = {10.5530/pj.2021.13.190}, author = {Suryati and Dira Hefni and Fatma Sri Wahyuni and Dachriyanus} } @article {1332, title = {The Effect of Jatropha curcas L Seed Extract on AST/ALT Activity and The Central Vein Thickness in Liver}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {66-72}, type = {Original Article}, chapter = {66}, abstract = {

Background: Jatropha is known as anti-inflammatory, antioxidant, anti-fungal, anti-cancer, and has coagulant activity. Jatropha curcas (Jatropha curcas L.) contains toxic compounds such as cursin, ricin and gallic acid. The liver has an important role in the process of metabolism and detoxification of xenobiotic substances. Repeated exposure to toxic compounds can damage hepatic hepatocytes. If the hepatocyte cells are injured, the AST/ALT enzyme is excreted and goes into the blood vessels, as an indicator of liver damage. This is also indicated by changes in the thickness of the central veins. This study aims to determine the effect of giving jatropha seed extract (Jatropha curcas L.) on AST/ALT activity and the central vein thickness in the liver. Materials and Methods: The research design was experimental, using male rats (Rattus novergicus L) Sprague Dawley strain. The rats were given Jatropha seed extract at doses of 0, 5, 25, 50, and 250 mg/ KgBW for 28 days. To assess liver damage, measurements of AST/ ALT activity and thickness of the central vein in the liver were performed. Results: Jatropha seed extract increased ALT activity at doses of 25.50, and 250 mg / KgBW compared to the control group (1.207; 1.62; 1.548 IU/L/ mg tissue x 10-3); and increased AST activity at doses of 5, 25, 50, and 250 mg / KgBW compared to the control group (0.769; 0.974; 1.449; 1.185 IU/L/ mg tissue x 10-3); Central vein thickness increased at doses of 25 and 50 mg/KgBW (6.17 and 4.9 μm) (Kruskal Wallis; p\> 0.05). Conclusion: Jatropha curcas L. seed extract increased the activity of AST/ALT and the thickness of the central vein in the liver.

}, keywords = {AST/ALT, Jatropha, Liver, The central vein thickness}, doi = {10.5530/pj.2021.13.10}, author = {Faradila Amirabagya and Rr Ayu Fitri Hapsari and Endah Wulandari} } @article {1387, title = {Effect of Tender Coconut Water (TCW) on TNF-α, IL-1 and IL-6 in Streptozotocin (STZ) and Nicotinamid (NA) Induced Diabetic Rats}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {500-505}, type = {Research Article}, chapter = {500}, abstract = {

Background: Diabetes Mellitus (DM) is characterized by an increase in blood sugar levels also known as hyperglycemia. Continuous hyperglycemia can increase the production of Reactive Oxygen Species (ROS). ROS causes oxidative stress and increases the formation of TNF-α expression as a marker of inflammation. Tender coconut water is rich in sources of free amino acids, L-arginine and vitamin C which can prevent oxidative stress. Aim and Objectives: This research to investigate the effect of tender coconut water on TNF-α, IL-1 and IL-6 in Streptozotocin (STZ) and Nicotinamid (NA) induced diabetic rats. Material and Methods: Experimental research design using posttest control group design. Twenty four male wistar strain rats were used in this study were divided randomly into 4 groups, which are group K1 (control); K2 (DM type 2); K3 (DM type 2+ Glibenclamid 0,18mg/200grBW); K4 (DM type 2+ tender coconut water 8mL/200gr BW). Type 2 Diabetes Mellitus were induced using Streptozotocin (STZ) 65mg/kg body weight and Nicotinamid 230 mg/kg body weight. The administration of tender coconut water were given on day 3 after DM condition is reached, given daily for 4 weeks with dose of 8 mL/200 gr BW. Data on of TNF-α , IL-1 and IL-6 levels were analyzed by One Way Anova. Result: Average TNF-α level, IL-1 level and IL-6 level in Group 2 increased compared to Group 1 , in Group 3 it decreased compared to Group 2 as well as in Group 4 .The results of the analysis has the p values \<0.05. Conclusion: Administration of tender coconut water can be decreasing of TNF-α, IL-1 and IL-6 levels in wistar strain male rats with type 2 Diabetes Mellitus.

}, keywords = {Diabetes mellitus, IL-1, IL-6, Tender coconut water, TNF-α}, doi = {10.5530/pj.2021.13.63}, author = {Siti Thomas Zulaikhah and Joko Wahyuwibowo and Mochammad Navi Suharto and Bagus Haruno Enggartiasto and Mohammad Iqbal Raka Ortanto and Arrizki Azka Pratama} } @article {1377, title = {Ergosterol Isolated from Agaricus blazei Murill N-Hexane Extracts as Potential Anticancer MCF-7 Activity}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {418-426}, type = {Original Article}, chapter = {418}, abstract = {

Extracts and some of the Agaricus blazrei Murill isolates have potential anticancer. Ergosterol isolate from Amaouroderma rude can also inhibit the growth of MDA-MB-231 cancer cells through apoptotic pathways by increasing FOXO3 expression, while its potency against MCF-7 cells has not been reported. The purpose of this study was to isolate, determine the structure, determine the anticancer activity of MCF-7 cells, and the isolate mechanism by apoptosis from one of isolates the n-hexane A.blazei Murill extracts. This research method includes the isolation of compounds from A.blazei Murill extract by chromatography method guided using Bioactivity Guided Isolation. The structure elucidation of structure isolates used UV, NMR and MS spectroscopy. Anticancer activity test using the MTT cytotoxic test. Eludation of UV, NMR and MS structures showed a ergostrerol. The anticancer activity test showed IC50 values of 43.10 μg/ mL with the strong cytotoxic category. The mechanism of action is to increase apoptosis induction through inhibition of the cell cycle in the G2/ M phase. The conclusion of the isolated compound was ergosterol with an IC50 value of 43.10 μg / mL with an increased apoptosis induction mechanism through inhibition of the cell cycle in the G2/ M phase.

}, keywords = {Agaricus blazei, Apoptosis, Egosterol, MCF-7 cells, Murill extract}, doi = {10.5530/pj.2021.13.53}, author = {Misgiati Misgiati and Aty Widyawaruyanti and Sentot Joko Raharjo and Sukardiman Sukardiman} } @article {1620, title = {Evaluation of Xanthine Oxidase Inhibitory and Antioxidant Activities of Three Organs of Idat (Cratoxylum glaucum Korth.) and Correlation with Phytochemical Cont}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {July 2021}, pages = {971-976}, type = {Research Article}, chapter = {971}, abstract = {

Introduction: Idat (Cratoxylum glaucum Korth.), belonging to the genus Cratoxylum, is known to contain xanthone, quinone, flavonoids, and other phenolic compounds. Objectives: to analyze total phenolic, flavonoid, antioxidant activity, and inhibitory xanthine oxidase activities of leaves, stem, and cortex of idat. Methods: Extraction of leaves, stem, and cortex of idat was carried out by reflux using n-hexane, ethyl acetate, and ethanol as a solvent. Antioxidant activity was tested by the DPPH method and calculated to get the antioxidant activity index (AAI). Determination of total phenolic and flavonoid levels by ultraviolet-visible spectrophotometry. Results: Spectrophotometers measured the inhibitory activity on xanthine oxidase in 96-well plates with allopurinol as standard. Total phenolic and flavonoid content from C. glaucum extracts varied from 6.62 to 48.77 g GAE/g extract and 1.54 - 25.96 g QE/100 g extract, respectively. The ethanol extracts of leaves, stem, and cortex were very strong antioxidant activity with Antioxidant Activity Index (AAI) values 3.89; 4.55; 10.50, meanwhile AAI of ascorbic acid and quercetin 9.46 and 14.81 respectively. The n-hexane of stem extract had a strong xanthine oxidase inhibitory activity with the IC50 was 36.64 μg/ml, while allopurinol was 5.02 μg/ml. Conclusions: Total phenolic content contributed to antioxidant activity. Phenolic compounds in leaves extracts led to the xanthine oxidase inhibitory and antioxidant activities. The extract of C. glaucum was active as an antioxidant and potentially an inhibitor of xanthine oxidase agents.

}, keywords = {Antioxidant, Cratoxylum glaucum, Xanthine oxidase inhibitory}, doi = {10.5530/pj.2021.13.125}, author = {Dadang Juanda and Irda Fidrianny and Komar Ruslan Wirasutisna and Muhamad Insanu} } @article {1373, title = {Gas Chromatography-Mass Spectrometry (GC-MS) Assay of Bio- Active Compounds and Phytochemical Analyses in Three Species of Apocynaceae}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {383-392}, type = {Original Article}, chapter = {383}, abstract = {

Objective: Gas chromatography coupled with mass spectrometry (GC-MS) was used to analyze for phytochemicals and bioactive compounds in three species of Apocynacae, Gongronema latifolium, Vincetoxicum rossicum and Marsdenia edulis commonly found in tropical rainforest vegetation and used as food and traditional medicine by locals. Methods and Materials: Phytochemical analysis and GC-MS were carried out using leaf samples of the species following standard protocols. Quantitative phytochemical data were analyzed using analysis of variance (ANOVA) and significance tested at 5\% level of probability. Bioactive compounds were identified by comparing the retention times with those of authentic compounds and spectral data obtained from National Institute of Standards and Technology (NIST) library. Results: Phytochemical analysis revealed presence of alkaloids, glycosides, tannins, saponins, terpenes, steroids, flavonoids and phenols. Among the three species, Gongronema latifolium was highest in flavonoids (28.40 \%), Vincetoxicum rossicum was highest in steroids (17.25 \%) while Marsdenia edulis was highest in terpenoids (18.17 \%). GC-MS profiling of the species revealed biologically functional compounds with therapeutic properties including linoleic acid, phytol, neophytadiene, n-hexadecanoic acid, squalene, transfarnesol, 5-pentadecen-7-yne, and mercaptoacetic acid. Conclusion: The array of bioactive compounds present in the three species especially Gongronema latifolium, commonly used as food indicated their utility in pharmacognosy and drug manufacture. This is the first report of GC-MS based metabolite profiling to detect the various bioactive compounds in methanolic extracts of Vincetoxicum rossicum and Marsdenia edulis. We therefore recommend these species for further study in drug discovery trials.

}, keywords = {Drug discovery, Gongronema latifolium, Marsdenia edulis, Phytochemical profiling, Underutilized species, Vincetoxicum rossicum}, doi = {10.5530/pj.2021.13.49}, author = {Peggy Willie and Edak A Uyoh and Peter O Aikpokpodion} } @article {1679, title = {Hepatoprotective Effect of Ganoderma applanatum Crude Polysaccharides on Carbon Tetrachloride-Induced Early Liver Fibrosis in Mice}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1428-1436}, type = {Research Article}, chapter = {1428}, abstract = {

Introduction: Liver fibrosis is a treatable disease when still at early stages. Ganoderma applanatum plays role as alternative medicine. The fungi have antioxidant, anti-inflammatory, and anticancer bioactivities. This study aimed to evaluate the hepatoprotective effects of G. applanatum crude polysaccharides (GACP) on liver fibrosis due to CCl4 induction. Methods: This study was divided into six groups. BALB/c mice were given CCl4 dissolved in olive oil (2 mL/kg; 1:3) intraperitoneally (i.p) twice a week for four weeks to produce a liver fibrosis model. Distilled water (control group), silymarin 100 mg/kg and GACP 25, 50, 100 mg/kg were given once daily for four weeks. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis-α (TNF-α) and interleukin-6 (IL-6) were determined by enzyme-linked immunosorbent assay (ELISA) kit. Histopathology was stained by hematoxylin \& eosin (H\&E) and Masson{\textquoteright}s trichome. Results: The administration of GACP effectively prevented ALT, AST, TNF-α, and IL-6 levels from high elevation. Additionally, the GACP had protective effect after liver histological analysis exhibited less injury in the liver tissue. Conclusion: The hepatoprotective effect of GACP on liver fibrosis is mainly due to avoid high elevation of pro-inflammatory cytokine. This result revealed that GACP could be a potential hepatoprotective agent for later clinical therapy.

}, keywords = {Anti-inflammatory, Carbon tetrachloride, Crude polysaccharides, Fibrosis, Ganoderma applanatum}, doi = {10.5530/pj.2021.13.181}, author = {Raden Joko Kuncoroningrat Susilo and Dwi Winarni and Saikhu Akhmad Husen and Suhailah Hayaza and Sri Puji Astuti Wahyuningsih and Ruey-An Doong and Win Darmanto} } @article {1643, title = {In Silico Analysis of Pinostrobin Derivatives from Boesenbergia pandurata on ErbB4 Kinase Target and QSPR Linear Models to Predict Drug Clearance for Searching Anti-Breast Cancer Drug Candidates}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1143-1149}, type = {Original Article}, chapter = {1143}, abstract = {

Background: ErbB4 is a member of ErbB family of receptor tyrosine kinases (RTKs) and plays an important role in resistance to ErbB2 inhibitors. Objective: This study aimed to design a pinostrobin derivative with activity as an ErbB4 inhibitor and to establish a quantitative structure-property relationship (QSPR) of pinostrobin and its derivatives to predict drug clearance. Materials and Methods: In this research, an in silico study was conducted on pinostrobin and its derivatives by predicting the prediction of activity spectra for substances (PASS) with PASS online, followed by molecular docking using the AutoDockTools 4.2.6 program on ErbB4 protein kinase and visualizing the docking results using the Discovery Studio Visualizer software. While the study of QSPR pinostrobin and its derivatives was determined using physicochemical parameters with clearance (CLtot) using SPSS. Results: From the data obtained, 5-O-2- phenylacetylpinostrobin has a high affinity for ErbB4 protein with a free energy of binding (ΔG) -10.37 kcal/mol and an inhibition constant (Ki) of 26.06 nM. Conclusion: Probability {\textquotedblleft}to be active{\textquotedblright} (Pa) 5-O-2- phenylacetylpinostrobin of 0.595 for kinase inhibitors and 0.666 for apoptosis agonists, thus becoming candidates for breast cancer drugs. The QSPR model can be used to predict the properties of molecules such as CLtot, this will be useful in the drug design process. The best QSPR regression equation for pinostrobin and its derivatives is Log (1/CLtot) = 0.705 Log S + 0.035 MR + 0.375. This equation can be used as a reference in predicting CLtot.

}, keywords = {5-O-acylpinostrobin, Molecular docking, PASS, Pharmacokinetic, Physicochemical properties}, doi = {10.5530/pj.2021.13.147}, author = {Ersanda Nurma Praditapuspa and Siswandono and Tri Widiandani} } @article {1695, title = {In vitro Wound Healing Potential and Antimicrobial Activity of Clerodendrum Inerme Leave Extracts}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1542-1548}, type = {Review Article}, chapter = {1542}, abstract = {

Background: Clerodendrum inerme is a medicinal plant which exhibited many pharmacological effects. However, wound healing property of this plant has not been investigated. Objective: The present study was designed to evaluate wound healing and antimicrobial activities of C. inerme leaves using the in vitro model with Human keratinocytes (HaCaT). Methods: Cytotoxicity and wound healing effects were determined by MTT and scratch wound healing assay. Antimicrobial activities against cutaneous flora and clinical isolates of bacteria were investigated by broth microdilution assay. Results: The results showed that the water extract of C. inerme leaves did not exhibit cytotoxic effects on HaCaT cells, while ethanol extracts at higher concentrations significantly decreased cell growth with an IC50 value of 386.8 {\textpm} 87.1 μg/mL. The water extract and the lowest concentration (6.25 μg/mL) of the ethanol extract significantly increased percentage of wound closure compared with the untreated group. The water and ethanol extracts of C. inerme displayed a broad spectrum of antibacterial activity, inhibiting growth of Staphylococcus aureus, Staphylococcus.epidermidis, Escherichia coli, and Pseudomonas aeruginosa. The water extract displayed remarkable activity against methicillin-resistant S. aureus with MIC and MBC values ranging from 0.39 to 1.56 μg/mL. Notably, it provided stronger antibacterial activity than vancomycin and also showed antifungal activity against C. albicans. Conclusion: This study confirms the potential of C. inerme leaves for wound healing and antimicrobial therapy and supports the continued utilization of C. inerme leaves in traditional medicine. Further studies are needed to clarify the molecular mechanisms through which it exerts such biological effects.

}, keywords = {Antimicrobial activity, Clerodendrum inerme, Keratinocytes, Scratch assay, Wound Healing}, doi = {10.5530/pj.2021.13.196}, author = {Sueptrakool Wisessombat and Malatee Tayeh} } @article {1687, title = {An Investigation into the Use of an Herbal Labor Induction Tincture Containing Black Cohosh, Cramp Bark, Partridgeberry, and Motherwort on Contractile Responses Produced from Isolated Strips of Mouse Uterine Tissues}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1478-1487}, type = {Research Article}, chapter = {1478}, abstract = {

Introduction: Alternative solutions in the form of herbal remedies meant to ease or expedite the process of labor have often been pursued and administered but have seldom been quantitatively tested for efficacy. Published research has shown some validation for some commonly used herbs such as blue cohosh (Caulophyllum thalictroides), red raspberry (Rubus idaeus), castor bean (Ricinus communis) and evening primrose (Oenothera biennis) on isolated mouse uterine strips. Methods: This study tested an array of herbs used by midwives in a labor induction tincture, which included black cohosh (Actaea racemosa), cramp bark (Viburnum opulus), partridgeberry (Mitchella repens), and motherwort (Leonurus cardiaca). Each of these four herbs were prepared as aqueous extracts and applied individually to uterine tissues in an organ bath apparatus. Results: Results and analyses indicated that all treatment tissues produced significant increases in contractile forces when compared to their own endogenous motilities (p\<0.0001). These responses, however, were not significantly different among the four herbal treatments, but were comparable to those produced by 10-5 M oxytocin. Conclusions: This study provides empirical support for the use of these plants as herbal uterotonics, and it is plausible that these results, collected in vitro, may support the use of such a tincture to enhance or augment labor in vivo. Consideration of the active constituents found in each herbal, their concentrations, solubilities, cell signalling pathways, and the potential to interact in a synergistic or attenuating manner would be beneficial prior to recommending a given herbal for use at an organismal level.

}, keywords = {Complimentary therapies; Herbal medicine; Labor, Induced; Midwifery; Oxytocics}, doi = {10.5530/pj.2021.13.188}, author = {Clayton Neuenschwander and Katrina Wu and Teresa DeGolier} } @article {1361, title = {Microparticles of Herbal Extracts with Antioxidant Activity}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {285-295}, type = {Review Article}, chapter = {285}, abstract = {

Plants that have antioxidant content have been shown to have efficacy on the body, antioxidants have several drawbacks including being sensitive to environmental factors such as light, heat, pH, and oxygen. Microencapsulation is a method that has several advantages including providing several benefits, namely microparticles formulated to protect the core from the environment, cover up discomfort, maintain volatility or cell survival, separate incompatible substances, protect the body from side effects, and optimize, extend, or target drug effects. The choice of the type of polymer used will determine the characteristics of the microparticles produced, therefore a suitable coating material is needed to produce the microparticles. This review article was made to find out the results of research conducted in the manufacture of microparticles by using polymers which are expected to be useful to provide information on the basis of the selection of polymers and methods of making microparticles produced to maintain the stability of substances that are efficacious as antioxidants. Based on the results of the literature search, microencapsulation is a method used to maintain the stability of antioxidant content that has a therapeutic effect.

}, keywords = {Antioxidant, Extract, Microparticle, Polymer}, doi = {10.5530/pj.2021.13.38}, author = {Zulham and Gofarana Wilar and Yasmiwar Susilawati and Anas Subarnas and Anis Yohana Chaerunisaa} } @article {1654, title = {Microwave-Assisted Extraction and Identification of γ- Oryzanol from Rice Bran (Oryza sativa L. cv Ciliwung)}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1242-1247}, type = {Research Article}, chapter = {1242}, abstract = {

Rice Bran (Oryza sativa L. cv Cilliwung) is a by-product of the rice milling process, consisting of an outer layer of rice grains with the whole seed institute. Rice bran oil is a result of rice bran extraction. Rice bran oil contains antioxidant compounds, one of which is oryzanol, so this study aims to determine the levels of or-oryzanol in rice bran. The stages of the research included sample preparation, extraction using Microwave-Assisted Extraction (MAE), followed by Vacuum Liquid Column Chromatography (VLCC) was extracted using the MAE method produced 30 mL of black-brown rice bran oil extract. A total of 8 grams of rice bran extract was obtained at VLCC using eluent (n-hexane: ethyl acetate) with ratios 9:1, 7:3, and 1:1, respectively. Then the five factions obtained were characterized using HPLC, and GC/MS identified the mass of the γ-oryzanol compound.

}, keywords = {Microwave assisted extraction, Rice Bran (Oryza sativa L. cv Cilliwung), Secondary metabolite, γ- Oryzanol}, doi = {10.5530/pj.2021.13.157}, author = {Maryono and Netti Herawati and Meuthia Aulia Farhani Gaffar and Sartini and Aliyah and Elly Wahyudin} } @article {1368, title = {Nutritional and Functional Potential of Selliera radicans Cav., a Chilean Native Halophyte}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {341-346}, type = {Original Article}, chapter = {314}, abstract = {

Background: Selliera radicans was recognized as one of the foods consumed by the oldest human settlement in America (Monte Verde, Chile) that had a diet with a high component of plants. Objective: This study aims at investigating nutritional and functional characteristics of S. radicans, a native halophyte from Chile. Materials and Methods: An analysis of total protein, carbohydrate, ash, and moisture from S. radicans leaves was performed, using standard methods. The content of macro and micronutrients was quantified by atomic absorption spectrometry. The inulin content was carried out based on the Seliwanoff reactions. S. radicans leaves were extracted with methanol and the total content of phenolic and flavonoids and antioxidant activity were evaluated by spectroscopic method. Results: Leaves from cultivated plants proved to be a suitable source of proteins (7.5 \% on DW), ash (6.8 \% on DW), and a wide range of macro and micronutrients, where Ca, K, and Na had the highest values. In addition, inulin (2.3\% on DW), total phenolics (63.4 GAE/g LDW) and flavonoids (21.8 QE/g LDW), and antioxidant capacity (10 TE/g LDW) were noted. Conclusions: According to the results, cultivated S. radicans leaves are promising sources of food with beneficial health properties.

}, keywords = {antioxidant activity, Goodeniaceae, Inulin, Macro and Micronutrients, Total flavonoids, Total Phenolics}, doi = {10.5530/pj.2021.13.44}, author = {Maria del Pilar Caramantin Soriano and Flavia Schiappacasse and Patricio Pe{\~n}ailillo and Jaime Tapia and Sergio Wehinger and Camilo A Valenzuela-Vasquez and Sarvia M Dur{\'a}n-Pe{\~n}a} } @article {1699, title = {Optimization of Solvent Extraction of the Leaves of Premna serratifolia and Quantitation of Premnaodoroside A by Validated TLC-densitometric Method}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {December 2021}, pages = {1656-1663}, type = {Research Article}, chapter = {1656}, abstract = {

Introduction: The leaves of Premna serratifolia have been widely used in traditional medicine. Its major constituents were iridoid glycosides and phenolic compounds. Antioxidation was one of its reported bioactivities. The aim of this study was to optimize the ethanol concentration used to obtain high quality extracts. Methods: Three sources of leaf samples were separately extracted by 0, 20, 40, 60, 80 and 100\% ethanol. Their content of premnaodoroside A, the major glycoside, was determined by a TLCdensitometric method developed in this study. The content of total phenolic compounds and antioxidants were also determined by Folin-Ciocalteu and DPPH scavenging methods, respectively. Results: The developed TLC-densitometric method showed a good linearity (R2 \> 0.99) in the range of 0.11-0.872 μg/spot. Its limit of detection and limit of quantitation were 0.13 and 0.04 μg/spot, respectively. The method had satisfactory specificity, precision and accuracy. The analysis results of all extracts showed that the 100\% ethanolic extracts exhibited the highest contents of premnaodoroside A (3.23{\textendash}5.25\%), total phenolic compounds (7.56-8.24\% GAE) and antioxidants (4.92{\textendash}6.66\% AAE), but its extractive yields (13.57-19.57\%) were the lowest. Yields of the 40\% and the 80\% ethanolic extracts were the highest in two samples (36.37-45.96\%) and one sample (47.53\%), respectively. Considering of the extractive ability, the 80\% ethanol could extract the highest amounts of all investigated compounds from plant material. Conclusion: The 100\% ethanolic extract possessed the highest contents of the interesting compounds, but the 80\% ethanolic extract had a higher extractive yield. Their benefits and drawbacks must be considered, depending on further application.

}, keywords = {Antioxidation, Extraction optimization, Premna serratifolia, premnaodoroside A, TLCdensitometry, Total phenolic.}, doi = {10.5530/pj.2021.13.213}, author = {Khin Su Yee and Tasamaporn Sukwattanasinit and Uthai Sotanaphun and Penpun Wetwitayaklung and Bunyapa Wangwattana and Worrakanya Narakornwit} } @article {1669, title = {Phytochemical Composition and Antibacterial Activities of Syzygium polyanthum Methanolic Leaves Extract}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1355-1358}, type = {Original Article}, chapter = {1355}, abstract = {

Background: Since a long time ago, Syzygium polyanthum has become traditional herb for health, and thus many studies done to confirm the medical effectiveness. Objectives: This present study was conducted to evaluate phytochemical compounds and antibacterial activities of methanolic extract of S. polyanthum leaves. Materials and Methods: In the present study, standard methods of determination were used to determine the phytochemical compounds of S. polyanthum. The methanol extract of S. polyanthum leaves were subjected for antimicrobial activity. Antibacterial activity was evaluated against five bacterial strains by determining minimum inhibitory concentration (MIC) and zone of inhibition. Diameters of the zone of inhibition were compared with standard antibiotics. Results: Phytochemical analyses qualitatively reveal the presence of alkaloids, saponin, terpenoids and steroid. The extract was found to exert antibacterial activity against four tested bacteria which are S. aureus, S. pyogenes, MRSA and K. pneumoniae except for E. coli. The zones of inhibition shown by the disc diffusion method for S. aureus were between 8.6 to14.0 mm, S. pyogenes were between 8.4 to 12.0 mm, MRSA was between 10.0 to 13.2 mm and K. pneumoniae were between 8.0 to 10.6 mm. Meanwhile, there was no zone of inhibition was observed for E. coli. The MIC was determined ranging from 6.25 to 12.5 mg/ml against all the tested bacteria. The highest value of MIC showed by S. aureus, S. pyogenes, MRSA and K. pneumoniae which is 6.25 mg/mL. Conclusion: The tested leaves extract showed promising antibacterial activity against both Gram positive and Gram-negative bacteria. Phytochemical screening revealed the presence of alkaloids, saponin, terpenoids and steroid in methanolic leaves extract qualitatively and these compounds could be responsible for antibacterial properties of leaves extract of S. polyanthum.

}, keywords = {Syzygium polyanthum; Phytochemical; Antibacterial}, doi = {10.5530/pj.2021.13.171}, author = {Noor Zarina Abd Wahab and Nur Saidatul Aqilah Ja{\textquoteright}afar} } @article {1619, title = {Phytochemicals and Antioxidant Activities Evaluation of Origanum vulgare (L.) Stem Bark Extracts}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {July 2021}, pages = {965-970}, type = {Research Article}, chapter = {965}, abstract = {

The present study aimed to evaluate phytochemical and antioxidant activity (in vitro and in vivo) of Origanum vulgare (L.) ethanolic extract. The phytochemical test was assessed using the Clule method in ethanol, ethyl acetate, and hexane. In vitro evaluation of antioxidant activity was determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) as an artificial free radical activity. In vivo test was conducted to evaluate the effect of malondialdehyde (MDA) level in blood plasma during maximum physical activity treatment. In vivo test was done using 25 male Sprague Dawley rats in pre and post-test control group design. The phytochemical test of O. vulgare ethanol extract was showed some compounds, such as a flavonoid, alkaloid, triterpenoid/steroid, essential oil, and tannin, then in ethyl acetate and hexane. In vitro assay showed that O. vulgare extract has strong antioxidant activity with an IC50 value of 133.47 μg/mL. While in the in vivo test, the most effective dosage is 20 mg/200 gr B.W., represented by a significant decrease of MDA level (0.509 nmol/mL) before and after treatment. So, the ethanolic extract of clove has potency as an herbal antioxidant because of the low level of IC50 and can decrease the MDA level.

}, keywords = {2, 2-diphenyl-1-picrylhydrazyl, antioxidant activity, Malondialdehyde, Origanum vulgare (L.), Phytochemical}, doi = {10.5530/pj.2021.13.124}, author = {Rosmalena and Putu Ayu Widyastuti and Fatmawaty Yazid and Neneng Siti Silfi Ambarwati and Islamudin Ahmad} } @article {1380, title = {Potential Inhibition by Phaleria macrocarpa Leaves Ethanol Extract on Ki-67 Expression in Distal Colon Mouse}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {443-449}, type = {Research Article}, chapter = {443}, abstract = {

Ulcerative colitis (UC) has been an important aspect of an incurable chronic inflammatory disease over the last few decades. To find useful therapies for UC, one of which is herbal therapy, many researches have been conducted. Due to its anti-inflammatory effects, Phaleria macrocarpa (PM), an Indonesian indigenous herb, is considered to be the alternative therapy for UC. Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is then used in this research to determine its effect on UC by using Ki-67 as a marker of proliferation. PMLEE was created from dry PM content undergoing maceration. The animals were classified into six categories: normal, positive control, negative control and PMLEE group (100, 200, 300 mg/kgBW). PMLEE was then injected for 7 consecutive days into BALB/c mice that were caused by dextran sodium sulphate (DSS). DSS is used for modeling UC in the colon tissue of mice. All mice were terminated and then stained with anti-Ki-67 after their colons were extracted. Subsequently, the stained parts were analyzed with ImageJ based on the color intensity produced by the results of H-score. Based on H-score, PMLEE 300mg and 200mg has significantly decreased the expression of Ki-67 compare to the negative control (p=0.001 and p=0.01). PMLEE also has a tendency to be dose dependent based on the significant difference from PMLEE 300mg and 100mg (p=0.002). It then concludes that PMLEE is related to Ki-67 expression in cells, as it was inversely proportional in this analysis.

}, keywords = {Dextran sodium sulphate, Inflamation, Ki- 67, Mahkota Dewa (Phaleria macrocarpa)}, doi = {10.5530/pj.2021.13.56}, author = {Kusmardi Kusmardi and Elvan Wiyarta and Ari Estuningtyas and Nurhuda Sahar and Yurnadi Hanafi Midoen and Aryo Tedjo and Alfred Pakpahan} } @article {1647, title = {The Potential Interaction of Ethionamide-Thyroid Hormone Receptor Induces Hypothyroidism}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1174-1179}, type = {Research Article}, chapter = {1174}, abstract = {

Background: Hypothyroidism is a common side effect found in patients with multidrug-resistant tuberculosis taking ethionamide. The mechanism of ethionamide-induced hypothyroidism is potentially caused by the structure of ethionamide compounds chemically similar to thioamide, such as propylthiouracil (C7H8N2S), which inhibits thyroid hormone synthesis. However, hypothyroidism is caused not only by a lack of production but also by signaling alteration. Thyroid hormone action is mediated by thyroid hormone receptors (TRs), members of the nuclear receptor superfamily that regulate their target genes. Unfortunately, there are limited studies on the potential interaction of ethionamide with TRs. Objective: In the present study, we want to elaborate on the potential interaction of ethionamide with TRs which might alter the thyroid hormone genomic regulation. Methods: Molecular docking studies were used to evaluate the potential interaction between ethionamide with TRα and TRβ. Results: The molecular docking results on TRα showed more than one hydrogen bond{\textendash}steric interaction formed from the ethionamide{\textendash}amino acid residue interaction. Ethionamide{\textendash}TRβ interaction showed more than one steric interaction, but the hydrogen bonds are not visualized. The docking score between ethionamide and TRα is -7.373 kcal/ mol and higher than its interaction with TRβ. Conclusion: These findings indicate that ethionamide can interact with TRα and TRβ. However, the ethionamide{\textendash}TRα interaction is stronger than ethionamide{\textendash}TRβ interaction. Our study reports a novel mechanism of action of ethionamide-induced hypothyroidism.

}, keywords = {Ethionamide, Hypothyroidism, Molecular docking, TRα, TRβ}, doi = {10.5530/pj.2021.13.150}, author = {Ronny Lesmana and Firyali Rahmani Shidqi and Hanna Goenawan and Iwan Setiawan and Marisca Evalina Gondokesumo and Farida Suhud and Nasrul Wathoni} } @article {1326, title = {Potential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {23-29}, type = {Original Article}, chapter = {23}, abstract = {

Ulcerative colitis (UC) is a part of incurable chronic inflammatory disease that has gained importance over the past few decades. A lot of research has been done to find effective treatments for UC, one of which is herbal medicine. Phaleria macrocarpa (PM), an Indonesian native plant, is thought to be an alternative therapy for UC because of its anti-inflammatory properties. Therefore, in this research, Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is used to assess its effect on UC by using Caspase-3 as apoptosis marker. PMLEE was made from dried material of PM that undergo maceration. Animals were separated into six groups: normal, negative control, positive control, and PMLEE groups (100, 200, 300 mg/kgBW). PMLEE was then injected to BALB/c mice that have been induced by dextran sodium sulphate (DSS) for 7 consecutive days. DSS is used to model UC in mice colon tissue. All animals were sacrificed and their colons were collected then stained with anti-Caspase-3. The stained sections were subsequently examined with ImageJ based on color intensity which generated H-Score as the results. Based on H-Score of each group, PMLEE 300mg has significantly upregulate the expression of Caspase-3 compare to the negative control (p=0.015). PMLEE also has a tendency to be dose dependent based on the significant difference between PMLEE doses. Therefore, it concludes that PMLEE is able to upregulate the expression of Caspase-3 in colon cells as in this study it was directly proportional. Key words: Mahkota Dewa, Inflammation, Apoptosis, Ulcerative colitis.

}, keywords = {Apoptosis, Inflammation, Mahkota Dewa, Ulcerative colitis}, doi = {10.5530/pj.2021.13.4}, author = {Kusmardi Kusmardi and Elvan Wiyarta and Ari Estuningtyas and Nurhuda Sahar and Yurnadi Hanafi Midoen and Aryo Tedjo} } @article {1412, title = {Qualitative and Quantitative Analysis of 70\% Ethanol Extract from Ruta angustifolia for Developing Anti-Hepatitis C Agents}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {682-687}, type = {Original Article}, chapter = {682}, abstract = {

Background: Medicinal plants are potential sources for drug candidates. It possesses with various metabolites which have many pharmacology effects. Ruta angustifolia is one of medicinal plants that has been used traditionally for liver disease. Previous study it has been demonstrated to inhibit hepatitis C virus under in vitro cell culture. It decreased protein NS3 level and gave synergistic effect in combination with simeprevir and telaprevir. This plant provides a prospective candidate to develop as anti-HCV Objective: This study evaluates the phytochemistry screening for qualitative assay and determine the concentration of rutin as marker compound for developing R. angustifolia extract as anti-HCV agent. Materials and Methods: R. angustifolia leaves were extracted with 70\% of ethanol. Extract and rutin were analysis their anti-HCV activity by in vitro culture cells of Huh7it. The concentration of rutin was determine by TLC densitometry. Results: The 70\% ethanol extract of R. angustifolia dan rutin exhibit anti-HCV activities with IC50 value of 2.9 {\textpm} 0.8 μg/ml and 28.1 {\textpm} 5.6 μg/ml, respectively. Screening phytochemistry demonstrated to contain flavonoid, terpenoid, alkaloid and polyphenols. TLC densitometry analysis yield the concentration of rutin in extract 0.06 \%. Conclusion: Extract of 70\% ethanol of R. angustifolia has a potential anti-HCV activity. Extract of R. angustifolia may provide a good candidate for developing anti-HCV agents.

}, keywords = {Hepatitis C Virus, Infectious disease, Medicinal plants, Medicine, Ruta angustifolia, Rutin}, doi = {10.5530/pj.2021.13.87}, author = {Tutik Sri Wahyuni and Adita Ayu Permanasari and Lidya Tumewu and Aty Widyawaruyanti and Achmad Fuad Hafid} } @article {1618, title = {Repairing of Renal Tubules in Diabetic Rats (Rattus norvegicus) Diabetes After Administration of Golden Sea Cucumber (Stichopus hermanii)}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {July 2021}, pages = {958-964}, type = {Research Article}, chapter = {958}, abstract = {

This study aims to analyze the improvement of the histopathological picture of renal tubules in diabetic rats after being given golden sea cucumber extract (Stichopus hermanii). This research uses experimental design laboratories with a post-test only control group design method. The sampling used simple random sampling with 25 white rats divided into five groups, namely groups that were given standard feed without STZ-induced, STZ-induced group, STZ-induced group and given golden sea cucumber extract at a dose of 4.25 mg/kg BW for 21 days, STZinduced group and given golden sea cucumber extract at a dose of 8.5 mg/kg BW for 21 days, and STZ-induced group and given gold sea cucumber extract at a dose of 12.75 mg/kg BW for 21 days. The data on the observation of each group{\textquoteright}s kidney histopathology was carried out at the end of the session. The results of the analysis using the Kruskal-Wallis Test showed significant results, the Mann-Whitney Test showed significant differences between the STZinduced group and the golden sea cucumber extract (Stichopus hermanii) dose of 12.75 mg/kg BW (x=7.4 mg/dl) decreased significantly (p=0.001) compared to the STZ-only-induced group (x=12.6 mg/dl). Administration of golden sea cucumber extract (Stichopus hermanii) at a dose of 12.75 mg/kg BW of rats for 21 days lowered the degree of tubular damage to the kidney rectus of white rats (Rattus norvegicus) male streptozotocin-induced strains.

}, keywords = {Diabetic rat, Stichopus hermanii, Tubular injury}, doi = {10.5530/pj.2021.13.123}, author = {Herin Setianingsih and Satria Erda Wisnumarta and Sareh Arjono Tjandra} } @article {1333, title = {Standardization of Eleutherine bulbosa Urb. Bulbs and Total Flavonoid Content from Three Locations in Kalimantan, Indonesia}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {73-80}, type = {Original Article}, chapter = {73}, abstract = {

Background: Dayak Onion (Eleutherine bulbosa Urb.) is a typical plant of Kalimantan which is traditionally used by the Dayak community as a medicinal plant. Dayak onion bulbs have been proven had many pharmacology activities. Objective: This study aims to determine the nonspesific and specific parameters of 70\% ethanol extract of Eleutherine bulbosa Urb. Total flavonoids was also quantified Methods:. Eleutherine bulbosa Urb was extracted with maseration method used etanol 70 \% as solvent. Determination of non-spesific includes by determined specific gravity, water content, total ash content, acid insoluble ash content, residual sovents, heavy metanol contamination, microbial contamination, mold and yeast contamination. Determination of specific parameters included extract identity, organoleptic extract, water/ethanol soluble content, chromatography profile. Total flavonoid content were quantified with colorimetric method. Results: there were no significance difference between nonspesific and specific parameters Eleutherine bulbosa Urb from three different locations. Measurement of total phenol content and total flavonoid content respectively form South Borneo were ; 6,499 {\textpm} 0,5248 mg QE/g extract, from central borneo were 7,585 {\textpm} 0,0437 mgQE/g extract, and from east borneo were 5,035 mg {\textpm} 0,3887 mgQE/g extract. Conclusion: it can be concluded that bulbs of Eleutherine bulbosa Urb from three locations have characters to similar between each other and bulbs of Eleutherine bulbosa Urb form central borneo had the highest total flavonoid content.

}, keywords = {Eleutherine bulbosa Urb., Flavonoids, Non-specific parameters, Specific parameters, standardization}, doi = {10.5530/pj.2021.13.11}, author = {Rahmi Muthia and Helmina Wati and Wahyudin Bin Jamaludin and Kartini and Finna Setiawan and Muhammad Fikri and Abdul Wahhab} } @article {1675, title = {Study of Sungkai (Peronema canescens, Jack) Leaf Extract Activity as an Immunostimulators With In vivo and In vitro Methods}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1397-1407}, type = {Original Article}, chapter = {1397}, abstract = {

Introduction: Sungkai (Peronema canescens, Jack.) contains polysaccharides, terpenoids, alkaloids, and polyphenols which have pharmacological activity as immunostimulants. Objective: This study aimed to see how the effect of Sungkai extract as an immunostimulant agent was carried out in vitro and in vivo. Materials and Methods: This study was conducted using two methods, namely in vivo and in vitro. In vivo research method was conducted to test the activity and phagocytic capacity of macrophage cells, the percentage of leukocytes, and the total number of leukocytes. This study used 30 male white mice as the test animals that were randomly divided into 5 treatment groups. Each group was consisting of 6 mice which were given different treatments. The negative control group was given with the 0.5\% NaCMC suspension, the mice test substance group was given with the suspension of Sungkai ethanol extract with various doses of 800, 400, and 200 mg/kgBW, and lastly the comparison group was given with the Stimuno in a dose of 50 mg/kg orally for 7 days. On day 8, blood was taken from the mice{\textquoteright}s vein to count the number and percentage of its leukocytes, then followed by the intraperitoneal injection of a Staphylococcus aureus bacteria suspension. After 1 hour of administration of the bacterial suspension, the peritoneal fluid was taken to be observed for its activity and phagocytic capacity of macrophage cells. The in vitro research method was used to test the viability and immunostimulatory activity of RAW 264.7 cells with the Sungkai extraction at the concentration of 1.10, 100 g/m. This cell viability test using the microtetrazolium (MTT) method aims to see whether the Sungkai sample used is safe and not toxic to RAW 264.7 cells by observing at the cell viability value that should exceed \>90\%. The concentration of Sungkai extraction at 1.10, 100 g/mL was found to be safe and non-toxic to RAW 264.7 cells with a viability value of \>90\%. Thus, this concentration of Sungkai extraction can be performed for its immunostimulatory activity test on LPS induced of RAW 264.7 cells by observing their levels of IL-6 and TNF-α. (proinflammatory cytokines) were compared with the LPS alone as a control using the sandwich ELISA (Enzyme-Linked Immunosorbent Assay) method. Results: The observations were analyzed by one-way ANOVA and Duncan{\textquoteright}s follow-up test (significance was taken at p\<0.05). The results showed that variations in concentration increased significantly (p\<0.05) on the activity and phagocytic capacity of macrophage cells, along with the total leukocyte cells. The percentage of leukocytes showed that the cells had a significant increase (p\<0.05). It was found that the Sungkai extraction on 1.10, 100 g/mL could significantly increase the concentration of TNF- and IL-6 (p\<0.05) which were tested by one-way ANOVA and followed by Duncan{\textquoteright}s post hoc test. Conclusion: Sungkai leaf extract (Peronemacanescsens Jack.) in a dose of800, 400, and 200 mg/kgBW has an immunostimulant effect both in vivo and in vitro.

}, keywords = {Cell viability, immunostimulant, Jack), LPS (lipopolysaccharide), Macrophages, MTT (Microtetrazolium), Phagocytosis, RAW 264.7 cells, Sungkai (Peronema canescens, total and percentage of leukocytes}, doi = {10.5530/pj.2021.13.177}, author = {Dwisari Dillasamola and Yufri Aldi and Fatma Sri Wahyuni and Rauza Sukma Rita and Dachriyanus and Salman Umar and Harrizul Rivai} } @article {1721, title = {Styrylpyrone Derivative from Goniothalamus sp.: A Powerful Drug for Fighting Against Herpes Simplex Virus Type 1}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {December 2021}, pages = {1598-1606}, type = {Original Article}, chapter = {1598}, abstract = {

Background: The emergence of drug resistance towards Herpes Simplex Virus Type 1 (HSV-1) has encouraged scientists to develop novel lower toxicity and highly effective anti-HSV drugs. Styrylpyrone derivative (SPD) is a bioactive compound isolated from the roots and leaves of Goniothalamus sp. It is believed that this compound possesses antiviral properties against HSV-1. Objective: This paper introduces the interaction of SPD towards HSV-1 through in silico study of molecular docking and molecular dynamic simulation. Materials and Methods: Molecular docking is a computational tool which is used to study the molecular interaction between two or more structures. ADME/T properties of the SPD were generated using the SwissADME online tool in which SPD was found to have a good pharmacokinetic profile. Results: Molecular docking study revealed that SPD has a high docking score of -7.9 Kcal/mol. SPD has a strong affinity with the thymidine kinase (PDB id: 1OF1) producing hydrogen bond and non-polar interaction at the target point of amino acid residue. Conclusion: Molecular docking analysis provides new insight into the structure-based design of SPD compounds with better antiviral activity against HSV-1.

}, keywords = {Antiviral, Herpes Virus type 1 (HSV-1), in silico approaches, Molecular docking and Styrylpyrone derivative.}, doi = {10.5530/pj.2021.13.206}, author = {Syaza Safia Fouzi and Noor Zarina Abd Wahab and Leong Chee Yan and Nazlina Ibrahim} } @article {1702, title = {Syzygium polyanthum (Wight.) Walp Ethanol Extract Decreased Malondialdehyde Level in Type 2 Diabetic Patients}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {December 2021}, pages = {1557-1561}, type = {Original Article}, chapter = {1557}, abstract = {

Background: Beside conventional oral antidiabetic, many diabetic patients used plant as an alternative medicine. One of the plants is Syzygium polyanthum Wight. Walp. Previous study showed that ethanolic extract of Syzygium polyanthum (Wight.) Walp leaves (EESP) is safe to be consumed by healthy volunteer. Aim: The present study was conducted to investigate the effect of EESP on malondialdehyde (MDA) level in type 2 diabetes mellitus volunteers. Materials and Methods: EESP was obtained by maceration then formulated in capsules (weight of 350 mg). A total of 12 volunteers were randomly given EESP or Placebo/ Pl (amylum) once daily for 14 days in conjunction with metformin 500 mg twice daily. Hemoglobin (Hb), liver and kidney function and MDA were measured at the beginning and at the end of the study. Results: After 14 days administration of EESP, Hb 12.52{\textpm} 1.23 (g/dL), SGOT 24.16{\textpm}13.57 (U/L), SGPT 27.50 {\textpm} 20.52 (U/L), ureum 23.12{\textpm}13.27 (mg/dL), creatinine 0.71 {\textpm} 0.23 (mg/dL) while MDA 1041.63 {\textpm} 615.66 ng/mL in EESP treated group. The reduction of MDA level in EESP-treated group (24\%) were higher than Pl-treated group (16\%). Conclusion: S. polyanthum leaf extract is potential as antioxidant in type 2 diabetes mellitus patients.

}, keywords = {Diabetes mellitus; Extract; Malondialdehyde; S. polyanthum}, doi = {10.5530/pj.2021.13.198}, author = {Tri Widyawati and M. Aron Pase and Milahayati Daulay and Imam Bagus Sumantri} } @article {1137, title = {Acute Toxicity Tests of Fermented Robusta Green Coffee Using Zebrafish Embryos (Danio rerio)}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {485-492 }, type = {Original Article}, chapter = {485 }, abstract = {

Background: Green coffee beans are coffee beans of coffee fruit that have not yet been roasted. The use of green coffee beans as a weight-loss agent has been widely used worldwide, but nowadays there is a new way to enjoy coffee by adding kombucha culture to it, or what is known as kombucha coffee. The development of this fermented product preparation still requires a more in-depth study, one of which is related to the toxicity of the kombucha green coffee preparation. Objective: This research was aimed to determine LC50 values for robusta green coffee fermented with kombucha culture using zebrafish (Danio rerio) embryos using an in vivo method. Methods: This study observed the development of 20 zebrafish embryos administered one of five different concentrations of kombucha coffee preparation 24 hours up to 96 hours, with the experiment repeated three times. The percentage of embryo deaths was observed and analyzed using a probit model for LC50 concentration and analyzed using IBM SPSS Ver 23. Results: An LC50 for kombucha green coffee of 1294.29 ppm was obtained. Statistical tests on the concentration of kombucha coffee extracts were not significant differences with p-values \> 0.05. Conclusion: The LC50 of robusta green coffee extract using zebrafish embryos of 1294.29 ppm included in the safe category.

}, keywords = {Green coffee, Kombucha, Toxicity, Zebrafish embryos}, doi = {10.5530/pj.2020.12.75 }, author = {Tedjo Narko and Marlia Singgih Wibowo and Sophi Damayanti and Indra Wibowo} } @article {1160, title = {The Alkaloid Fraction from Melicope latifolia Leaves Inhibits Hepatitis C Virus}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {535-540 }, type = {Research Article}, chapter = {535}, abstract = {

Introduction: Hepatitis C Virus (HCV) is a major health problem, which infects approximately 170 million people among worldwide population. Moreover, there is no vaccine available to prevent HCV infection and the current anti-HCV drugs have not covered all the various genotypes and subtypes. Meanwhile, medicinal plants have been widely used to treat a variety of infectious disease. Our previous study reported that ethanol extract of Melicope latifolia has been shown to exert anti-HCV activity towards a number of different virus genotypes with mainly inhibition mechanism at the entry step. Further separation was needed to purify and identify the active anti-HCV constituent using bioactivity-guided isolation method. Materials and Methods: In vitro Anti-HCV assay was performed using hepatocyte cell line (Huh7it) and HCV genotype 2a (JFH1). The purification of M. latifolia ethanol extract (B1F) was done by liquid-liquid fractionation, vacuum liquid chromatography (VLC), and high-performance liquid chromatography (HPLC). The active fraction was further identified by thin layer chromatography (TLC) and the major constituent was determined by nuclear magnetic resonance (NMR) spectra data analysis. Results: The fractionation of M. latifolia leaves ethanol extract resulted an alkaloid fraction (B1F D2H.3) containing a major constituent N-methylflindersine. This alkaloid fraction was active to reduce HCV JFH1 with an inhibition concentration (IC50) value of 6.21 {\textmu}g/mL, a cytotoxicity concentration (CC50) value of 82.64 {\textmu}g/mL, and a selectivity index value of 13.31. Conclusion: An alkaloid fraction of M. latifolia (B1F D2H.3) was known to have major compound named N-methylflindersine. This alkaloid fraction exhibited strong anti-HCV against JFH1 in vitro. The results indicated that this alkaloid fraction may a good candidate for anti-HCV agent.

}, keywords = {Anti-HCV, Melicope latifolia, N-methylflindersine}, doi = {10.5530/pj.2020.12.81 }, author = {Dwi Susiloningrum and Adita Ayu Permanasari and Myrna Adianti and Lidya Tumewu and Tutik Sri Wahyuni and Mulyadi Tanjung and Aty Widyawaruyanti and Achmad Fuad Hafid} } @article {1226, title = {Antifungal Activity of Neolignan Derivatives from Eusideroxylon zwageri Against Pathogenic Fungus Microsporum gypseum}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {August 2020}, pages = {993-999}, type = {Original Article}, chapter = {993}, abstract = {

Introduction: Bulian wood (Eusideroxylon zwageri) known as iron wood. It was known as wood source which fungi and insect resistant. These effects were interconnected with secondary metabolites contained within. Methods: Eusideroxylon zwageri wood powder was macerated with methanol and fractinated with n-Hexane, dichloromethane, ethyl acetate. Eusiderin I and Compound B were isolated from n-hexane fraction, while Compound C were isolated from dichloromethane fraction. Eusiderin I, compound A and Compound B were characterized using melting point, UV spectroscopy and compared with previous data. Antifungal activity test was conducted with Microsporum gypseum using paper disc method. Results: A research on antifungal activity of Neolignan derivatives from Eusideroxylon zwageri against pathogenic fungus Microsporum gypseum had been carried out. Eusiderin I, Compound B and Compound C were isolated from wood of Eusideroxylon zwageri. All three compounds are white crystals with melting point in such 99-100 oC, 110-112 oC, 98-99 oC, respectively and UV spectrum data is similar to reference. The antifungal activity test of Eusiderin I, Compound B and Compound C from Bulian wood (Eusideroxylon zwageri) to phatogen fungus of Microsporum gypseum showed that with five different concentrations (5, 25, 50, 100 and 200 ppm), Eusiderin I was a potent antifungal because it had a strong activity in inhibiting the Microsporum gypseum growth. The 5 days incubation test result showed that 50 ppm Eusiderin I could inhibit the Microsporum gypseum colony growth. The 100 ppm Eusiderin I gave the most effective inhibition precentage because it could inhibit the Microsporum gypseum colony growth (= 93.9\%). Conclusion: Based on this data, Eusiderin I can be indicated an antifungal candidate.

}, keywords = {Antifungal, Eusiderin, Eusideroxylon zwageri, Microsporum gypseum}, doi = {10.5530/pj.2020.12.140}, author = {Anis Yohana Chaerunisaa and Muhaimin Muhaimin and Syamsurizal Syamsurizal and Harizon Harizon and Tiana Milanda and Imam Adi Wicaksono} } @article {1312, title = {Antihypertensive, Antidiabetic, Antioxidant and Cytotoxic Activities of Indonesian Traditional Medicine}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {November 2020}, pages = {1623-1629}, type = {Research Article}, chapter = {1623}, abstract = {

Background: Indonesian people have long used herbal medicine (jamu) to overcome various diseases, including hypertension and diabetes mellitus. Hypertension and diabetes mellitus are two diseases that are directly related and require proper and thorough management. Objectives: The present study investigated the antihypertensive, antidiabetic, and cytotoxic activities ethanol extracts of Indonesian traditional medicine (jamu). Material and Methods: Jamu was extracted by maceration using ethanol. Antihypertensive and antidiabetic activity investigated by measurement of ACE inhibitor, an alpha-glucosidase inhibitor, and antioxidant activity at a concentration ranging from 125-1000 μg/mL, respectively, by in vitro method. Cytotoxic evaluation of the extract was carried out using Brine Shrimp Lethality Test (BSLT). Results: measurements of ACE inhibitors, alpha-glucosidase inhibitor and antioxidant activity showed that herbal extracts had ACE inhibitors, alpha-glucosidase inhibitors, and antioxidant activity with IC50 values of 292.15 μg/mL, 36.13 μg/mL, and 24.43 μg/mL respectively. Ethanol extract of herbal medicine (jamu) exerts a cytotoxic effect on larvae of shrimp Artemia salina with an IC50 value of 215.04 μg/mL. Conclusion: Jamu extract has antihypertensive and antidiabetic activity in vitro and cytotoxic effects.

}, keywords = {ACE inhibitors, Alpha-glucosidase inhibitors, Antioxidant, Cytotoxic, Jamu}, doi = {10.5530/pj.2020.12.222}, author = {Aprilita Rina Yanti Eff and Hermanus Ehe Hurit and Sri Teguh Rahayu and Muhammad Unggul Januarko and Putu Gita Maya WM} } @article {1184, title = {Antimalarial Activity of Flavonoid Compound Isolated from Leaves of Artocarpus altilis}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {835-842}, type = {Research Article}, chapter = {835}, abstract = {

Introduction: Artocarpus altilis leaves extract has previously been reported as a potential antimalarial drug. Inhibition concentration (IC50) against P. falciparum and effective dose values (ED50) against P. berghei have been reported at 1.32 μg/ml and 0.82 mg/kg, respectively. The aim of this study is to identify the active compound from the ethanol extract of A. Altilis leaves against P. falciparum. Materials and Methods: The isolation of the active compound from the ethanol extract of A. altilis were conducted using chromatography methods, and the chemical structure of the isolated compounds was determined based on NMR and MS spectra data. Antimalarial assay was determined using microscopic method against P. falciparum 3D7 and molecular docking studies was performed using Molegro Virtual Docker version 5.5 program. Results: A flavonoid compound, class of dihydrochalcone was finally isolated from A. altilis and identified as 1-(2,4-dihydroxy phenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3- pentenyl)-2H-1-benzopyran-5-yl]-1-propanone (Compound-1). Antimalarial activity test revealed that the compound strongly inhibited P. falciparum growth, with IC50 value of 1.05 μM. An in silico study to determine the mechanism of action of the compound revealed the existence a 3.BPF receptor that possesses a cysteine protease inhibitor of falcipain-2. Conclusion: Compound-1 were isolated from the leaves of A. Altilis is a good candidate of new source in the development of antimalarial drugs. An animal study using this compound is recommended before a clinical trial.

}, keywords = {Artocarpus altilis, Cysteine protease inhibitor, Dihydrochalcones, P. falciparum 3D7}, doi = {10.5530/pj.2020.12.120}, author = {Agriana Rosmalina Hidayati and Aty Widyawaruyanti and Hilkatul Ilmi and Mulyadi Tanjung and Tri Widiandani and Siswandono and Din Syafruddin and Achmad Fuad Hafid} } @article {1223, title = {Anti-migration and Anti-invasion Abilities of Methanolic Leaves Extract of Clerodendrum Inerme on Lung Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {August 2020}, pages = {1024-1031}, type = {Original Article}, chapter = {1024}, abstract = {

Background: Clerodendrum inerme is a folk medicinal plant used in the treatment of various illnesses such as a cough, scrofulous infection, venereal infection, skin diseases and tumors. It exhibited many pharmacological effects including hepatoprotective, anti-inflammatory, antibacterial, anti-oxidant and anticancer properties. Objective: The purpose of this study was to investigate the influence of methanolic extract of C. inerme leaves on migration, invasion and adhesion activities on human lung adenocarcinoma. Materials and methods: Cytotoxicity, cell motility, migration, invasion and adhesion abilities were detected by MTT, wound healing, trans-well mobilization, modified Boyden chamber and cell adhesion assay, respectively. Results: The results demonstrated that up to 400 μg/mL methanolic leaves extract has low toxicity on A549 cells showing more than 50\% cell viability. At non-cytotoxic and sub-toxic doses (200 and 400 μg/mL) of methanolic leave extract significantly suppressed cell motility, migration, invasion and cell adhesion compared with the untreated control. Conclusion: These results suggested that methanolic leaves extract of C. inerme inhibited migration, invasion and adhesion of A549 cells. These findings showed new therapeutic potential for C. inerme in anti-metastatic therapy.

}, keywords = {Anti-invasion, Anti-migration, Clerodendrum inerme, Human lung adenocarcinoma}, doi = {10.5530/pj.2020.12.145}, author = {Malatee Tayeh and Poonsit Hiransai and Hathaichanok Kommen and Ramida Watanapokasin} } @article {1085, title = {Antioxidant and Tyrosinase Inhibitory Properties of an Aqueous Extract of Garcinia atroviridis Griff. ex. T. Anderson Fruit Pericarps}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {71-78}, type = {Original Article}, chapter = {71}, abstract = {

Background: Ultraviolet radiation (UVR) is the major cause for hyperpigmentation, and to prevent this natural products are increasingly being explored as potential skin whitening agents. The aim of this study was to determine the total phenolic and flavonoid content, free radical scavenging activity, anti-tyrosinase activity and the inhibition of melanin content in α-melanocyte stimulating hormone-induced B16F10 melanoma cells of an aqueous extract of Garcinia atroviridis Griff. ex. T. Anderson fruit pericarps. Methods: The aqueous extract was prepared by extraction with distilled water at 105oC for 60 min. Total phenolic and flavonoid content were determined using the Folin-Ciocalteau and aluminium chloride methods, respectively. Scavenging activity was assessed using 2,2-Diphennyl-1-picrylhydrazyl (DPPH) and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). Tyrosinase activity and melanin content were determined spectrophotometrically. Results: The results showed that the aqueous extract of Garcinia atroviridis fruit pericarps had a phenolic (26.33 {\textpm} 0.77 mg GAE/g plant extract) and flavonoid content (9.31 {\textpm} 0.40 mg QE/g plant extract). The aqueous extract of Garcinia atroviridis significantly inhibited mushroom tyrosinase activity (IC50 of 40.72 {\textpm} 1.83 μg/mL) and cellular tyrosinase activity (at a concentration of 125 μg/mL) in α-melanocyte stimulating hormone-induced B16F10 melanoma cells. The Garcinia atroviridis extract also suppressed melanin content at concentrations of 31.25-125 μg/mL. Correlations of mushroom tyrosinase inhibition with DPPH and ABTS scavenging activities were 0.8673 and 0.9468, respectively. Conclusion: Our findings show that an aqueous extract of Garcinia atroviridis fruit pericarps is a source of natural compounds and antioxidant capacity which can inhibit tyrosinase activity and melanin content. Thus, aqueous extracts of Garcinia atroviridis may be a potential source of skin whitening agents for hyperpigmentation.

}, keywords = {B16F10 cells, Garcinia atroviridis, Melanin, Tyrosinase activity, α-MSH}, doi = {10.5530/pj.2020.12.12}, author = {Moragot Chatatikun and Pitaksit Supjaroen and Patcharaporn Promlat and Chantanapa Chantarangkul and Sutida Waranuntakul and Jiraphat Nawarat and Jitbanjong Tangpong and Jitbanjong Tangpong} } @article {1235, title = {Antiviral Activity of Indonesian Medicinal Plants against Hepatitis B Virus}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {August 2020}, pages = {1108-1114}, type = {Research Article}, chapter = {1108}, abstract = {

Background: Hepatitis B virus (HBV) infects more than 300 million people globally and is a common cause of liver cancer. Current therapy using reverse transcriptase inhibitors require long-term treatment and the potential risk of development of drug-resistant viruses remains an important issue to be considered. Hence, the development of new drugs is critical. Traditional medicinal plants used for the treatment of infectious diseases may provide a viable option for the discovery of anti-HBV drug candidates. Objective: This study examined anti-HBV activity of 31 kinds of Indonesian plants. Materials and Methods: Crude extracts of various part of plants, leaves and stem, were obtained using dichloromethane and ethanol solvent. The effect on viral entry was examined by determining levels of HBsAg expression in the supernatants of HBV-infected HepG2-NTCP cells by ELISA. The effect on HBV replication was determined by measuring HBV DNAs amounts in Hep38.7-Tet cells by quantitative real-time PCR. Results: The extracts of Phyllantus niruri leaves and Curcuma xanthorrhiza showed reduction of strong HBsAg production from HepG2-NTCP cells with IC50 values of 170.48 and 270.51 μg/mL, respectively. Treatment of HepAD38.7-Tet cells with P. niruri and C. xanthorhiza at the highest concentration while avoiding cytotoxicity reduced extracellular HBV DNA levels by 70\% and 30 \% of the untreated control respectively. Conclusion: P. niruri inhibited both the entry and HBV replication, thus P. niruri is a promising candidate for anti-HBV drug development.

}, keywords = {Curcuma xanthorhiza, Hepatitis B Virus, Medicinal plants, Phyllanthus niruri}, doi = {10.5530/pj.2020.12.157}, author = {Tutik Sri Wahyuni and Adita Ayu Permanasari and Aty Widyawaruyanti and Hak Hotta and Chie Aoki-Utsubo and Achmad Fuad Hafid} } @article {1124, title = {Chemical Constituents, Antibacterial Activity and Mode of Action of Elephant Ginger (Zingiber officinale var. officinale) and Emprit Ginger Rhizome (Zingiber officinale var. amarum) Essential Oils}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {404-409}, type = {Research Article}, chapter = {404}, abstract = {

Introduction: Ginger (Zingiber officinale Rosc) is a spice plant, which is extensively used worldwide, and morphologically classified into three types, including the red, {\textquotedblleft}gajah{\textquotedblright} or {\textquotedblleft}elephant{\textquotedblright}, and {\textquotedblleft}emprit{\textquotedblright} ginger (common name in Indonesia). In addition, the extract and essential oils possess antibacterial pharmacological activities, due to the inherent constituents. The aim of this research, therefore, was to analyze the chemical constituents, test antibacterial activities, and observe the mode of action of elephant and emprit ginger rhizome essential oils. Methods: Essential oils isolation was conducted using water and steam distillation method, while microdilution method was adopted in the testing for antibacterial activities against Gram positive and negative bacteria. Furthermore, the mode of action was evaluated using Scanning Electron Microscopy (SEM). Results: The antibacterial activity demonstrated antibacterial activities in the essential oils of elephant and emprit ginger rhizome, with minimal inhibition concentrations (MIC) value of 250-1000 μg.mL-1 and minimal bacterial concentrations (MBC) value of 500-1000 μg.mL-1, while chemical evaluation showed the presence of 45 and 38 constituents, respectively. Conclusion: Both essential oils possess antibacterial activities against Gram positive and negative bacteria, with different strengths, which are based on chemical composition. Conversely, SEM micrographs demonstrated the ability for elephant and emprit ginger rhizome essential oils to change the morphology of bacteria.

}, keywords = {Antibacterial, Chemical constituents, Elephant ginger, Emprit ginger}, doi = {10.5530/pj.2020.12.62}, author = {Diki Prayugo Wibowo and Ria Mariani and Siti Uswatun Hasanah and Diah Lia Aulifa} } @article {1122, title = {Determination of Total Flavonoid Levels of Ethanol Extract Sesewanua Leaf (Clerodendrum Fragrans Wild) With Maceration Method Using UV-Vis Spectrofotometry}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {356-360}, type = {Research Article}, chapter = {356}, abstract = {

Introduction: Sesewanuwa (Clerodendrum fragrans Wild) is one of the plants with abundant flavonoid content in the leaves. The characteristic flavonoids with the two benzene ring groups cause the process of finding an appropriate extraction technique. Objective: This study aims to determine the total flavonoid levels of ethanol extract of sesewanuwa leaves obtained from maceration extraction methods. Method: This research was carried out by extracting the simplicia of sesewanuwa leaves by maceration method using 96\% ethanol solvent. Comparison between the simplicia and the solvent used is 1:7, then the extract obtained was carried out with initial qualitative identification of flavonoids with simple reagents and the total flavonoid levels were determined using UV-Vis spectrophotometry. Results: The results showed that the ethanol extract of sesewanuwa leaves obtained by maceration extraction method in qualitative and quantitative tests contained flavonoids with quercetin standard with a total content of 13.47\%.This research was carried out by extracting the simplicia of sesewanuwa leaves by maceration method using 96\% ethanol solvent. Comparison between the simplicia and the solvent used is 1:7, then the extract obtained was carried out with initial qualitative identification of flavonoids with simple reagents and the total flavonoid levels were determined using UV-Vis spectrophotometry. Conclusion: The results showed that the ethanol extract of sesewanuwa leaves obtained by maceration extraction method in qualitative and quantitative tests contained flavonoids with quercetin standard with a total content of 13.47\%.

}, keywords = {Maceration, Quercetin, Sesewanuwa, total flavonoid, UV-Vis spectrophotometry}, doi = {10.5530/pj.2020.12.56}, author = {Zulfiayu Sapiun and Paulus Pangalo and Arlan K Imran and Prisca Safriani Wicita and Rizka Puji Astuti Daud} } @article {1152, title = {Development and Validation of Stability Indicating HPLC Method for Determination of Caffeic Acid, Vitexin and Rosmarinic Acid in Thunbergia laurifolia Leaf Extract}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {611-618}, type = {Research Article}, chapter = {611}, abstract = {

Thunbergia laurifolia has been a popular herb used in Thai traditional medicine for detoxification and as antipyretic. It contains rosmarinic acid (RA), caffeic acid (CA) and vitexin as major compounds. In order to control the herbal quality, the stability indicating high-performance liquid chromatography (HPLC) was developed and validated. The stability study of compounds in T. laurifolia leaf extract was investigated. The chromatographic separation was performed using a reversed-phase C18 column and mobile phase consisted of 0.5\% acetic acid and methanol using a gradient elution with 1.0 mL/min flow rate. The detection wavelength was set at 330 nm. The method was validated for its linearity, precision, accuracy, limit of detection and limit of quantitation. Forced degradation of three compounds in extract showed that they were stable in oxidative condition, but highly labile under alkaline hydrolytic conditions. All three compounds in T. laurifolia leaf extract were stable at room temperature at least 3 months while a remarkable decrease of RA, vitexin and CA in the extract were found in accelerated condition. This finding could be applied for predicting the storage recommendation and expiry of T. laurifolia extract and its related pharmaceutical products.

}, keywords = {Force degradation, Phenolic compound, Phytochemical screening, Stabilityindicating method}, doi = {10.5530/pj.2020.12.91 }, author = {Nanthakarn Woottisin and Sumet Kongkiatpaiboon and Sophida Sukprasert and Korbtham Sathirakul} } @article {1100, title = {Effects of Pearl Grass Extract Capsules on Osteoarthritis Subject}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {303-310}, type = {Original Article}, chapter = {303}, abstract = {

Pearl grass extract, a standardized bioactive polar extract of the herbs of Hedyotis corymbosa, is preclinically proven to have an activity to osteoarthritis and rheumatoid arthritis properties in an animal model. The current clinical study has evaluated the efficacy and safety of Pearl grass extract of osteoarthritis subjects. This research was a pilot study of osteoarthritis subjects using Parallel double-blind study design. Subjects have normal renal and liver function, above 50 years of age, and has a sign and symptoms of osteoarthritis included in the study. In the treatment, Pearl grass capsules were given as one capsule once daily for eight weeks, throughout the study, the weekly assessment to evaluate the adverse event. Thirty subjects of mean age 55.90 {\textpm} 3.7 years were evaluable. Pearl grass extract capsules did not change renal and liver function in 56 days treatment. Pearl grass capsule markedly reduced the consumption of analgesic drug compared with placebo groups. Pearl grass capsules also decreased pain scale better compare with placebo.

}, keywords = {Clinical efficacy, Pearl grass extract capsules, Quercetin, VAS scale, WOMAC scale}, doi = {10.5530/pj.2020.12.48}, author = {Nita Sari Wijaya and Katrin Basah and Anton Bahtiar} } @article {1303, title = {Evaluation of Myrmecodia pendans Water Extracts on Hematology Profiles, Liver, Kidney Function and Malondialdehyde Level in Healthy Volunteer}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {November 2020}, pages = {1489-1493}, type = {Original Article}, chapter = {1489}, abstract = {

Background: Ant Nest (Myrmecodia pendans) is one of plants that have been used by locals in Indonesia to empirically treat various diseases. Ant Nest in vitro and in vivo studies on animals have been widely reported its pharmacological activities as an antioxidant. Unfortunately, scientific proofs reported on this plant as human medicine are still lacking. Aim: This study aimed to ensure effectiveness, and safety due to administration of Ant Nest Water Extract (ANWE) formulated in capsule. Materials and Methods: Twelve volunteers were divided into 2 groups, 6 volunteers in each group. Group I: Ant Nest Water Extract Capsule (ANWEC) in dose of 350 mg ANWE, and group II: Placebo Capsule (PC) which contained 350 mg amylum. Each groups given the capsule once daily for 28 days. The comparison was made between group I and group II. Hematology tests include hemoglobin, leucocyte and thrombocyte. Blood chemistry tests include SGOT, SGPT, total cholesterol, triglycerides, HDL, LDL, glucose, ureum (Ur) and creatinine (Cr). Malondialdehyde (MDA) level was measured at day-28 which was the last day of the intervention. Results: After 28 days administration of ANWE, the SGOT, SGPT, Ur, and Cr showed in normal level as follows SGOT (U/L): 19.2 {\textpm} 3.99; SGPT (U/L): 17.2 {\textpm} 6.80; Ur (mg/dL): 19.75 {\textpm} 3.66; Cr (mg/dL): 1.06 {\textpm} 0.13. MDA (ng/mL) level in ANWEC-treated group was significantly lower (117.2{\textpm}23.8) than PC (147.25{\textpm}18.7). There was no intolerable complaints during the observation. Conclusion: The study concluded that Water Extract of Ant Nest 350 mg has no damage to liver, kidney and hematology, so it was proven that this plant is safed to be consumed by human for its potency as antioxidant.

}, keywords = {Antioxidant, Malondialdehyde, Myrmecodia pendans, Safety}, doi = {10.5530/pj.2020.12.204}, author = {Tri Widyawati and M Aron Pase and Milahayati Daulay and Imam Bagus Sumantri and Nor Adlin Yusoff} } @article {1301, title = {Immunosuppressive Activity of Goat Kefir in a Rat Model with Bleomycin-induced Pulmonary Fibrosis}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {November 2020}, pages = {1594-1599}, type = {Research Article}, chapter = {1594}, abstract = {

Objective: This study aimed to investigate the immunomodulatory capacity of goat kefir on pulmonary fibrosis rat model. Material and Methods: Twenty-five male rats were randomly divided into five groups: one group only received induction with bleomycin (0.3 mg/rat) to induce pulmonary fibrosis; three groups were treated with different doses (2.5, 3.5, and 4.5 mL/200 g BW) of goat kefir, following the induction with bleomycin, for 30 days; and one group served as negative control, did not receive bleomycin induction as well as kefir. On day 30, all the animals were sacrificed. Plasma levels of TGF-β, IL-4, and IFN-y were measured using the ELISA method, and the expression of α-SMA in myofibroblast cells was examined with the help of immunohistochemistry assay. Results: Induction with bleomycin significantly elevated the expressions of TGF-β, IL-4, and IFN-y in comparison to the control group. Following the administration of kefir (3.5 and 4.5 mL/200 g BW), the concentration of TGF-β was significantly decreased (p\<0.05); whereas, the concentration of IFN-y increased slightly (p\<0.05) only in the group that received the 4.5 mL/200 g BW dose of kefir. In contrast, IL-4 exhibited increasing levels with higher doses of kefir (p\<0.05). The expression of α-SMA in myofibroblasts showed a tendency to decline following the administration of kefir, although this decline was not statistically significant. Conclusions: Goat kefir caused a reduction in the TGF-β levels in fibrosis conditions; however, the kefir elicited an immunosuppressive effect during the progression of the pulmonary fibrosis.

}, keywords = {Bleomycin, Cytokine, Immunomodulator, Kefir, Pulmonary fibrosis}, doi = {10.5530/pj.2020.12.218}, author = {Tias Pramesti Griana and Tri Yudani Mardining Raras and Karyono Mintaroem and Iin Noor Chozin and Catur Saptaning Wilujeng} } @article {1132, title = {In vitro Anti-Amebic Activity of Cage Xanthones from Cratoxylum sumatranum Stem Bark Against Entamoeba histolytica}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {452-458}, type = {Original Article}, chapter = {452}, abstract = {

Background: Amoebiasis is caused by Entamoeba histolytica, which is a pathogenic species living on human colon tissues. The development of new drugs for anti-amebic are still very needed for clinical treatment. Objective: This aims to identify the compounds in Cratoxylum sumatranum for their anti-amoeba activity. Materials and Methods: In this study we used bioactivity-guided isolation and structural analysis to identified anti-amebic compounds from dichloromethane extract of Cratoxylum sumatranum stem bark. Their anti-amebic activity was determined by an in vitro cell-based assay against Entamoeba histolytica and an enzymatic assay on NAD kinase. Results: Two known compounds from the cage xanthone groups, namely cochinchinoxanthone (1) and cochinchinone D (2), were isolated. The structures of the cage xanthone compounds were established by extensive spectroscopic data analysis. Compound (1) showed the greatest level of anti-amebic activity both in cell-based and enzymatic assay, yielding IC50 values of 4.57 and 12.17 μg/mL, respectively. In contrast, compound (2) yielded IC50 values of 5.19 and 12.60 μg/mL, respectively. Conclusion: When considering the demonstrated anti-amebic activities, it becomes apparent that these compounds, isolated from Cratoxylum sumatranum stem bark, have the potential to be further developed into effective anti-amebic medicine against Entamoeba histolytica.

}, keywords = {Amoebiasis, Bioactivity-guided isolation, Cratoxylum sumatranum, Entamoeba histolytica, NAD kinase}, doi = {10.5530/pj.2020.12.70}, author = {Fendi Yoga Wardana and Defi Kartika Sari and Myrna Adianti and Adita Ayu Permanasari and Lidya Tumewu and Tomoyoshi Nozaki and Aty Widyawaruyanti and Achmad Fuad Hafid} } @article {1087, title = {Iron-Folate Supplementation during Pregnancy for Prevent Oxidative Stress in Pregnant Rats: Level of MDA, Creatinine, Glucose, Erythrocite, Blood Pressure, Body Weight and Number of Offspring}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {186-191}, type = {Research Article}, chapter = {186}, abstract = {

Background: Iron and folic acid deficiency during pregnancy can increase oxidative stress and result in impaired intra-uterine growth, abortion and preeclampsia. Folate is trace nutrient that influent for essential role for epigenetic mechanism cues into changes in gene expression and had impact health development. This study aimed to determine the effect of several doses of iron and folate supplementation on level of: MDA, glucose, creatinine, maternal body weight and number and birth weight of offspring. Methods: This research was conducted in the laboratory of the Center for Food and Nutrition Studies, Gadjah Mada University Yogyakarta. Experimental research with posttest only control group design with a number of samples: 20 pregnant rats, divided randomly into 4 groups. The control group (C) was given standard feed (AIN-93G), KI: added iron 1,8 mg/200gBW and folic acid 0,0023mg/200gBW, KII: added iron 3,6 mg/200gBB and folic acid 0,0045 mg/200gBW, KIII : added iron 5,4mg/200gBW and folic acid 0,0068 mg/200gBW. Duration of treatment 20 days. Measurement of body weight, blood pressure and then taken blood samples at the 21st day for examination of MDA, glucose, creatinine, erythrocyte level. Sectio caesarean to performed the number and body weight of offspring. Data obtained were analyzed using one way Anova followed by Post hoc LSD. Results: there are significant different (p \<0.001). on level of : MDA, glucose, creatinine, maternal body weight, average number and fetal weight of offspring between treatment group compare to control group. Conclusion: Iron and folate suplementation during pregnancy can decreased level of oxidative stress and better pregnant outcome.

}, keywords = {Folic acid, Iron, MDA, Oxidative stress, Pregnancy}, doi = {10.5530/pj.2020.12.28}, author = {Joko Wahyuwibowo and Abdul Aziz and Eka Safitri and Minidian Fasitasari and Siti Thomas Zulaikhah} } @article {1098, title = {Leucaena leucocephala (Lam.) de Wit Seeds: A New Potential Source of Sulfhydryl Compounds}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {298-302}, type = {Original Article}, chapter = {298}, abstract = {

Background: Leucaena leucocephala (Lam.) de Wit seeds are considered the most widely consumed legumes by ruminants. The seeds contain around 1.5 mmol of thiol/sulfhydryl compounds per 100 g of dried seeds. The contents of the sulfhydryl compounds can act like glutathione. On the other hand, the intake of a high amount of Leucaena leucocephala (Lam.) de Wit seeds is limited by mimosine because it can induce toxicity and death in ruminants. Objective: The aim of this study was to determine sulfhydryl compound levels in Leucaena leucocephala (Lam.) de Wit seeds after the mimosine removal process. Materials and methods: Leucaena leucocephala (Lam.) de Wit seeds were soaked in aquadest for 24 hours and then dried at 40{\textdegree}C for 86 hours. The dried seeds were macerated at room temperature using 30\%, 50\%, 70\%, and 96\% (v/v) of ethanol as the solvent with a 1:10 solvent-to-solid ratio. Levels of dried extract yield were determined for sulfhydryl compounds and mimosine using a spectrophotometer. Results: The 30\% ethanol extract without soaking produced the highest levels of extract yield, but 70\% ethanol was the most effective solvent for extracting the maximum sulfhydryl and minimum mimosine levels. Conclusion: Ethanol solvent (70\%) can be used to extract maximum levels of sulfhydryl compound and minimum levels of mimosine from Leucaena leucocephala (Lam.) de Wit soaked seeds.

}, keywords = {Ethanol, Leucaena leucocephala (Lam.) de Wit, Mimosine, Sulfhydryl compounds}, doi = {10.5530/pj.2020.12.47}, author = {Sri Wardatun and Yahdiana Harahap and Abdul Mun{\textquoteright}im and Fadlina Chany Saputri and Noorwati Sutandyo} } @article {1233, title = {Nanoparticle Characterization of Allium sativum, Curcuma mangga and Acorus calamus as a Basic of Nanotechnology on Jamu Subur Kandungan Madura}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {August 2020}, pages = {1152-1159}, type = {Research Article}, chapter = {1152}, abstract = {

Introduction: The increasing of researcher attraction on the herbal drug after so long ignored due to difficulties in processing has opened a new door for the development of a novel of "jamu Subur Kandungan". However, the constraints that then faced in consuming "jamu Subur Kandungan", an herbal reproductive drug, are the solubility and poor absorption in the intestine. Therefore, this study aims to characterize nanoparticle of the combination of garlic (Allium sativum), temu mangga (Curcuma mangga) and jeringau (Acorus calamus) encapsulated by chitosan. Material and Methods: the simplicial of garlic (Allium sativum), temu mangga (Curcuma mangga) and jeringau (Acorus calamus) was purchased from Materia Medica Batu Malang Indonesia. Nanoparticle of combination of garlic, temu mangga and jeringau was produced by ionic gelation method. Nanoparticle characterization was assessed by Scanning electron microscopy (SEM), Spectrophotometer Fourier Transform Infra-Red (FTIR), Particle Size analyzer (PSA) and X-ray diffraction (XRD). Result: The ionic gelation method succeeded to make nanoparticle. The produced nanoparticle was around 438-1159 nm. The length of sonication has proven to make the particle size smaller. The particle size distribution of chitosan at the time of 90 min sonication and 150 min was classified as uneven because of the particle size clustered in the range 500-1000 nm and 3000-5000 nm. The hydroxyl (OH) group appeared at wave number 3429-2466 cm-1, while the amide functional group appeared at wave numbers (1648-1652 cm-1. Phosphate groups (P = O) also appeared, which is a TPP residue, at a wavenumber 1384 cm-1. Conclusion: Chitosan-garlic nanoparticles (Allium sativum), temu mangga (Curcuma mangga) and jeringau (Acorus calamus) were successfully produced with ionic gelation method.

}, keywords = {Characterization, Garlic, Ionic gelation, Jeringau, Nanoparticle, Temu mangga}, doi = {10.5530/pj.2020.12.162}, author = {Bayyinatul Muchtaromah and Didik Wahyudi and Mujahidin Ahmad and Rahmi Annisa} } @article {1065, title = {Nanoparticle Synthesis and Cytotoxicity of Kaempferia pandurata Roxb. Extract to the Growth of MDA-MB-231 Breast Cancer Cell Line}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {109-114}, type = {Research Article}, chapter = {109}, abstract = {

Breast cancer is the most common cancer worldwide and in Indonesia. Kaempferia pandurata Roxb. is a herbal plant from South-East Asia which is known for its ability to inhibit the growth of Estrogen Receptor (ER) + breast cancer cell line from the former study. However, its effect on ER- breast cancer cell lines had not been studied. Therefore, we want to examine the cytotoxicity effect of K. pandurata Roxb. on ER- breast cancer cell line (MDA-MB-231). Nanoparticle is a form of preparation that optimizes the activity of any compound to the targeted cell. Therefore, it is expected that it can increase the effectivity of anticancer in Kaempferia pandurata Roxb. In this study, the rhizome of K. pandurata Roxb. trituration was dried and extracted with n-hexane solvent. Nanoparticle of K. pandurata Roxb. was synthesized with CaCl2, chitosan, and alginate by stirring with a magnetic stirrer, adjusting pH, and centrifugation. Then, nanoparticle was analized by UV/VIS spectrofotometry and transmission electron microscopy (TEM). The cytotoxicity of K. pandurata Roxb. extract and nanoparticle were examined with MTT assay. The result of this test is data of inhibition percentage and IC50 value. The result showed that n-hexane extract of K. pandurata Roxb. is synthesized into nanoparticle form with 99,43\% yield percentage (entrapment value). Anticancer activity of n-hexane extract and nanoparticle of K. pandurata Roxb. is moderate with IC50 value of the extract is 87,23 μg/ml and the nanoparticle is 24,23 μg/ml. The nanoparticle{\textquoteright}s activity is better than the extract. n-Hexane extract and nanoparticle of K. pandurata Roxb. has cytotoxicity effects towards MDA-MB-231 cell line. Nanoparticle can increase the cytotoxicity effect of K. pandurata Roxb. extract because its hydrophobic feature and nanometer size.

}, keywords = {Breast cancer, Kaempferia pandurata Roxb., MDA-MB-231 cells, Nanoparticle, Temu Kunci}, doi = {10.5530/pj.2020.12.17}, author = {Risya Amelia Rahmawanti and Fadilah Fadilah and Brenda Cristie Edina and Lowilius Wiyono and Rafika Indah Paramita} } @article {1206, title = {Noni Juice (Morinda citrifolia) to Prevent Cancer Progression in Mice Induced DMBA and Cigarette Smoke Exposure}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {August 2020}, pages = {946-951}, type = {Original Article}, chapter = {946}, abstract = {

Introduction: Accumulation of polycyclic aromatic hydrocarbons (PAH) in the body commonly lead to degenerative disease such as cancer. This study aims to investigate the potential of Morinda citrifolia to maintain the immune system against toxic exposure. Materials and Methods: This study used Five weeks old male Balb/C mice as animal model. The 7,12-Dimethylbenz(a)anthracene (DMBA) was administrated for six weeks following with 3 days cigarette smoke (CS) exposure then treated with noni juice (M. citrifolia) for two weeks. Experimental animals were divided into six groups. Normal control (N); DMBA+CS; Cisplatin; D1; D2; and D3. Profil of CD4+TNFα+, CD11b+IL6+, CD11b+IFNγ+, CD4+CD25+ IL10+, NK+IL6+ cells was analyzed by flow cytometry and data was analyzed with one-way ANOVA and Post Hoc Tukey HSD test with a significance of p-values \< 0.05. Results: This study show that DMBA+CS induction increasing level of CD11b+IL6+, CD4+CD25+ IL-10+ and NK+ IL-6+ meanwhile decreasing CD4+TNFα+significantly (P\<0.5) than Normal group. Noni juice in dose 90 mg/Kg BW decrease cytokine pro-inflammation (IL-6 and IFNγ) both in macrophage and NK cell profile significantly (P\<0.05). Noni juice in 30 mg/Kg BW could improve the activation CD4+TNFα+ significantly (P\<0.05). Noni juice also has efficacy to control T regulator activation to prevent tumor escape. Conclusion: These results suggest that noni juice has anti-cancer potencies by maintain homeostasis of immune system and could be immune herbal supplement.

}, keywords = {Homeostatic, Immunotoxin, Inflammation, Noni juice, Tumor progression}, doi = {10.5530/pj.2020.12.134}, author = {Didin Wahyu Agustina and Mulya Dwi Wahyuningsih and Sri Widyarti and Aris Soewondo and Hideo Tsuboi and Muhaimin Rifa{\textquoteright}i} } @article {1091, title = {Phytochemical Composition and Evaluation of Marine Algal Sargassum polycystum for Antioxidant Activity and In Vitro Cytotoxicity on Hela Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {88-94}, type = {Original Article}, chapter = {88}, abstract = {

Introduction: Sargassum polycystum is one of marine algal which has a potent antioxidant anticancer activities. This research aims to investigate phytochemical composition, antioxidant activity and in vitro cytotoxicity of marine algal Sargassum polycystum on cervical HeLa cancer. Methods: Sargassum polycystum collected from Dompu beach, Lombok, Nusa Tenggara Barat Province, Indonesia, were extracted into organic solvent of n-hexane, ethylacetate, chloroform and ethanol, respectively. Subsequently, Sargassum polycystum extracts were applied for Thin Layer Chromatography (TLC) analysis, phytochemistry test, total phenolic and total flavonoid contents, as well as for antioxidant activity test by DPPH (2,2-diphenyl-1-picrylhydrazyl) method, and in vitro cytotoxicity evaluation on HeLa cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide) assay. Results: Phytochemical analysis of S. polycystum extracts are positive for metabolites of flavonoid, steroid, tannin and glycoside. TLC analysis revealed that S. polycystum extracts containing four phytochemical components. Ethylacetate extract of S. polycystum showed the highest total phenolic content, and exhibited greater antioxidant activity than ethanol extract. Total phenolic and total flavonoid content in ethylacetate extract are 548.61 μg/mL and 40.06 μg /mL, respectively. Ethylacetate extract of S. polycystum with IC50 value of 298.3 μg/mL is assigned to have a weak antioxidant activity against DPPH free radical. The results indicate that antioxidant activity of ethylacetate extracts of S. polycystum is directly correlated with its total phenolic and flavonoid content. Moreover, S. polycystum extracts demonstrated a strong anticancer activity on cervical HeLa cells with IC50 ranging from 38.3 μg/mL to 112.8 μg/mL. Conclusion: This work confirmed that S.polycystum are promising natural antioxidant and anti-cervical cancer agents.

}, keywords = {Antioxidant, Cytotoxicity, HeLa cells, phytochemisty, Sargassum polycystum}, doi = {10.5530/pj.2020.12.14}, author = {Ade Arsianti and Anton Bahtiar and Vincent Kharisma Wangsaputra and Norma Nur Azizah and Wilzar Fachri and Lince Dameria Nadapdap and Ajeng Megawati Fajrin and Hiroki Tanimoto and Kiyomi Kakiuchi} } @article {1139, title = {Pimpinella Treatment on Reducing Apoptosis of Kidney Cells Following UVB Radiation in Rats}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {503-509 }, type = {Original Article}, chapter = {503}, abstract = {

Introduction: Pimpinella alpina Molk (PM) is a botanical antioxidant was able to inhibit apoptosis in various cells. Apoptosis is a leading cause of tubular atrophy and therefore chronic kidney disease. However, the effect of PM on reducing apoptosis in kidney cells remains unclear. Objective: aim of this study to elucidate the effect of PM on reducing apoptosis in kidney cells. Methods: In the post test only control group design, 35 male rats were grouped into 7 comprise: NC-G, samples were neither exposure to UVB nor PM treatment; NG-7 and NG-15, all samples were only exposure to UVB irradiation for 7 days; P10-7, P15-7, P10-15, P15-15 groups, samples were exposure to UVB for 7 days and treated with PM for 7 and 15 days respectively. Bax and Caspase3 expression were assessed by rt-PCR and IHC staining method. Results: Statistical analysis showed that RNA-Bax and RNA-caspase3, Bax and caspase3 protein expression in P15-7, P10-15 and P15-15 were lower significantly compared to those of NG-7, p\<0.05, and no significant difference compared to those of NC-G, p \> 0.05. Conclusion: PM treatment with 100 and 150 mg/day for seven and fifteen days were able to decrease Bax and Caspase3 expression in kidney cells following UVB irradiation. Even, the decreased in Bax and caspase3 expression were comparable to normal.

}, keywords = {Apoptosis, Bax, Caspase3, Kidney Cells, Pimpinella alpina Molk}, doi = {10.5530/pj.2020.12.77 }, author = {Taufiqurrachman Nasihun and Eni Widayati} } @article {1123, title = {Study of the Effect of Lampeni (Ardisia humilis Vahl.) Planting Condition toward the Alpha-glucosidase Inhibition Activity in vitro}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {377-385}, type = {Research Article}, chapter = {377}, abstract = {

Background: The quality of a medicinal plant is influenced by agronomic conditions and harvesting time. Objective: This study aimed to evaluate the effect of planting method (openair (OA) and shedding house (SH)) and harvesting time (2, 4, 6 months) of Lampeni (Ardisia humilis Vahl.) toward the inhibitory activity of alpha-glucosidase. Methods: The Lampeni seedling were placed under controlled light conditions (SH) and on direct sun exposure (OA). Harvesting of the leaves was carried out at the age of 2, 4, and 6 months after plantation (2m, 4m, and 6m). Each leaves dry powder was refluxed with methanol 70\% and followed by liquid-liquid partition using n-hexane, ethyl acetate (EtOAc), and water. All samples were evaluated toward inhibition of the alpha-glucosidase enzyme in vitro. Total phenol levels were determined using Folin-Ciocalteu reagent. Results: The results showed that EtOAc fractions of both plantation techniques exhibited the highest inhibition of alpha-glucosidase. The highest activity was demonstrated by the 4m-OA-EtOAc fraction (IC50, 93.50 ppm) and followed by the 6m-OA-EtOAc fraction (IC50, 98.13 ppm). Based on the kinetic study, the inhibition type of the two most active samples were categorized as a non-competitive type. Total phenolic contents were decreased in the following order: 6m-SH-EtOAc \> 4m-OA-EtOAc \> 6m-OAEtOAc \> 4m-SH-EtOAc fraction. It was shown that there was no positive correlation between the strength of inhibition with total phenolic content. Conclusions: This study concluded that Lampeni at open-air plantation harvested on fourth months demonstrated the highest alpha-glucosidase inhibitory activity, although there was no positive correlation between the inhibition activity and phenolic content.

}, keywords = {Alpha-glucosidase, Ardisia humilis Vahl., Lampeni, Open-air, Shedding house, total phenolic}, doi = {10.5530/pj.2020.12.59}, author = {Sri Ningsih and Fifit Juniarti and Idah Rosidah and Adam Arditya Fajriawan and Kurnia Agustini and Syofi Rosmalawati and Agung Eru Wibowo and Erliana Sasikirana and Wahono Sumaryono} } @article {1108, title = {Synthesis, Characterization, and Cytotoxicity Evaluation of Gallic Acid Nanoparticles Towards Breast T47D Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {321-327}, type = {Original Article}, chapter = {321}, abstract = {

Introduction: Gallic acid is a naturally polyphenolic acid which shows cytotoxicity against several cancer cells, as well as it displays chemo-preventive activity which is attributed to its strong apoptosis- inducing and antioxidant effects. Thus, gallic acid has become an attractive substance to be further developed due to its strong cytotoxic activity. This study aimed to synthesize gallic acid nanoparticle coating with alginate-chitosan, and evaluate its cytotoxicity against breast T47D cancer cells. Methods: Gallic acid nanoparticle was synthesized using ionic gelation method. The yield, size and morphology of the nanoparticles were determined by UV-Vis Spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared (FTIR) spectroscopy. Cytotoxicity evaluation of gallic acid nanoparticle towards breast T47D cancer cell is carried out by MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay. Results: Spherical nanoparticles of gallic acid with the size of 100-200 nm has been successfully synthesized in 96\% of yield. Compared to gallic acid (IC50: 20.86 μg/mL) and alginate-chitosan nanoparticle (IC50: 38.46 μg/mL), gallic acid coating with alginate-chitosan nanoparticles demonstrated higher cytotoxicity towards breast T47D cancer cells with IC50 value of 9.03μg/mL. Conclusion: Our results clearly confirmed that gallic acid nanoparticles coating with alginate-chitosan showed a strong cytotoxicity towards breast T47D cancer cells, which is potential to be developed as a candidate for new anti-breast cancer agent.

}, keywords = {Cytotoxicity, Gallic acid, Nanoparticle, Synthesis, T47D cells}, doi = {10.5530/pj.2020.12.51}, author = {Ade Arsianti and Anton Bahtiar and Fadilah Fadilah and Vincent Kharisma Wangsaputra and Rafika Indah Paramita and Norma Nur Azizah and Lince Dameria Nadapdap and Ajeng Megawati Fajrin and Hiroki Tanimoto and Kiyomi Kakiuchi} } @article {1259, title = {Total Phenolic, Total Flavonoid and Phytochemical Screening by FTIR Spectroscopic of Standardized Extract of Mikania micrantha Leaf}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {September 2020}, pages = {1395-1401}, type = {Research Article}, chapter = {1395}, abstract = {

Background: Mikania micrantha is a great plant that has been used as raw material for traditional medicines. Objective: This paper aims to evaluate total phenols, total flavonoids, and phytochemical screening by FTIR spectroscopy of standardized extract of Mikania micrantha leaf to confirm its medicinal values. Materials and Methods: The leaves were extracted by maceration method using ethanol 96\% and evaporated by rotary evaporator. The determination of total phenolic and total flavonoid were performed by spectroscopic method. The phytochemical constituent was screened through the bioactive group of the chemical by FTIR analysis. Results: The total phenolic of extract of Mikania micrantha leaf ranged from 13.19{\textpm}0.74 to 34.24{\textpm}1.24 mg gallic acid equivalent/g and total flavonoid ranged from 1.11{\textpm}0.11 to 20.63{\textpm}0.16 mg quercetin/g. FTIR analysis confirmed the presence of O-H, aliphatic CH, and C=O functional group. Conclusion: the result of this study confirm that Mikania micrantha possesses the potential of bioactive compounds which are responsible for the biological activities that are useful for raw material of traditional medicines.

}, keywords = {FT-IR, Mikania micrantha, total flavonoid, total phenolic}, doi = {10.5530/pj.2020.12.193}, author = {Imam Bagus Sumantri and Henny Sri Wahyuni and Lolyta Fiti Mustanti} } @article {1016, title = {Anti-inflammatory Effect of Phyllanthus niruri L. from Indonesia (Pre-clinical Study)}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1347-1350}, type = {Research Article}, chapter = {1347}, abstract = {

Indonesian people often use Phyllanthus niruri L. (P. niruri L.) to treat pain. Pain is the one of symptoms of inflammation. Some people take traditional medicine to treat this pain, because some analgesic drugs have several adverse effects. The aim of this research is to explore the anti-inflammatory effect of ethyl asetat fraction of ethanolic extract of P. niruri L. (EAPN) from Indonesia in rats induced by carrageenan. A total of 25 rats were divided into 5 groups. Group 1 (diclofenac sodium 50 mg/kgbb), group 2 (aqueous extract), groups 3,4and 5 were ethyl asetat fraction at dosage of 250; 500 and 1000 mg/kgbw respectively. Group 1 was treated by diclofenac sodium at dose of 50 mg/kgbw, group II was treated by aqueous 2mL/rats, groups III, IV and V were treated by EAPN at dosage of 250; 500 and 1000mg/kgbw respectively. Thirty minutes later, all rats were injected by carrageenan 0,1 mL 1\% w/v sub cutaneous into back rat{\textquoteright}s left paw. Paw volumes were measured by Plethysmometer on minutes 0, 60, 90, 150, 210, 330. Area under the curve (AUC) were calculated from these results (AUC 0-30; AUC 0-90; 0-150; AUC 0-210 and AUC 0-330). The AUC of EAPN at dosage of 250; 500 and 1000mg/ kgbw smaller than negative control significantly (P\<0.05). EAPN contains alkaloids, flavonoids, terpenoids, saponins and triterpens. EAPN has anti-inflammatory effect in rats induced by carrageenan.

}, keywords = {Anti-inflammatory, Carrageenan, Phyllanthus niruri L.}, doi = {10.5530/pj.2019.11.208}, author = {Em Sutrisna and Maryati and Sri Wahyuni and Tanti Azizah S} } @article {921, title = {Antioxidant Activity of Ethanolic Extract and Various Fractions from Green Tea (Camellia sinensis L.) Leaves}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {July 2019}, pages = {771-776}, type = {Original Article}, chapter = {771}, abstract = {

Background: Free radicals are one of the causes that can cause premature aging and degenerative disease. To overcome this problem, the body needs antioxidant intake. Green tea (Camellia sinensis L.) leaves are one of the plants known as antioxidant agent due to its flavonoids and phenolic compounds or better known as catechin compounds. Catechin is polar flavonoid compounds so it is necessary to separate it from non-polar compounds so their antioxidant activity becomes effective. Objective: This study aims to determine antioxidant activity of ethanolic extract of green tea leaves and its fractions namely ethyl acetate and water fraction, and measure the total flavonoid content, total phenolic content and catechin content. Materials and Methods: Green tea leaves extracted using maceration method with 96\% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of n-hexane, ethyl acetate and water. Screening of flavonoid and phenolic and antioxidant activity was performed against the ethanolic extract, ethyl acetate fraction and water fraction. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultravioletvisible spectrophotometry with ascorbic acid as standard. Results: The ethanolic extract, ethyl acetate fraction and water fraction contains flavonoids and phenolic compounds. The IC50 value of ethanolic extract, ethyl acetate fraction and water fraction were 9.017; 3.926 and 7.408 μg/mL consecutively. The ethyl acetate fraction also showed better antioxidant activity than ascorbic acid (4.855 μg/mL). Conclusion: The ethanolic extract, ethyl acetate fraction and water fraction showed very powerful antioxidant activity but ethyl acetate fraction has the best antioxidant activity.

}, keywords = {antioxidant activity, DPPH method, Ethanolic extract, Ethyl acetate fraction, Green tea leaves}, doi = {10.5530/pj.2019.11.122}, author = {Karmika Indarti and Elsa Fitria Apriani and Agung Eru Wibowo and Partomuan Simanjuntak} } @article {1046, title = {Azadirachta indica: Antibacterial Activity of Neem Against Different Strains of Bacteria and their Active Constituents as Preventive in Various Diseases}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {November 2019}, pages = {1597-1604}, type = {Review Article }, chapter = {1597}, abstract = {

Neem has become valuable plant in the world which shows the solutions for hundreds to thousands problems. Azadirachta indica (Neem) is a rapidly growing evergreen well known tree found Pada generally in various regions of world like America, Africa and India. It has been widely used in Chinese, Ayurveda and Unani medicines across the world especially in Asians countries for the prevention and treatment of diseases. The different parts of neem plant contain biological compounds responsible for antibacterial, antiviral and antifungal activities. It is considered as safe medicinal plants and modulates the numerous biological processes without any adverse effect. Neem tree produces some active compounds which contain biological activities, parts of neem tree such as Root, bark, leaf, flower, seed and fruit together possesses biological activities. Various compounds have been obtained from various parts of neem. Biological activities of few of them have been studied. Hence, the article is aims to utilize the medicinal properties of whole neem plant in various disorders of mankind.

}, keywords = {Antibacterial, Antifungal, Azadirachta indica, Natural antibiotics, Natural products}, doi = {10.5530/pj.2019.11.244}, author = {Oscar Herrera-Calderon and Kainat Ejaz and Mahnoor Wajid and Muzzamil Shehzad and Johnny Aldo Tinco-Jayo and Edwin Enciso-Roca and C{\'e}sar Franco-Quino and Ricardo {\'A}ngel Yuli-Posadas and Victor Chumpitaz-Cerrate} } @article {824, title = {Cakile maritima Scop. Extracts Inhibit Caco2 and HeLa Human Carcinoma Cell Growth: GC-MS Analysis of an Anti-Proliferative Extract}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {February 2019}, pages = {258-266}, type = {Original Article}, chapter = {258}, abstract = {

Introduction: Exposure to high levels of antioxidants has been linked to the treatment and prevention of some cancers. Although Cakile maritima has a high antioxidant capacity, it is yet to be tested for the ability to inhibit the proliferation of cancer cells. Methods: Solvent extracts prepared from C. maritima plant material were analysed for antioxidant capacity by the DPPH free radical scavenging assay. Anti-proliferative activities against Caco2 and HeLa cancer cells were determined by an MTS based cell proliferation assay. Toxicity was determined by the Artemia franciscana bioassay. The most potent anti-proliferative extract (hexane) was further investigated using non-targeted GC-MS headspace analysis. Results: Good DPPH radical scavenging activity was calculated for all C. maritima extracts. The methanolic and ethyl acetate extracts had particularly strong antioxidant activity (IC50 of 4.7 and 3.4 μg/mL respectively). Interestingly, the hexane extract which had the lowest DPPH radical scavenging activity (IC50 13.6 μg/mL), was the most potent inhibitor or Caco2 and HeLa carcinoma cell growth, with IC50{\textquoteright}s of 12 and 126 μg/mL respectively. The ethyl acetate extract was also a potent inhibitor of proliferation (IC50 values of 185 and 468 μg/mL against Caco2 and HeLa, respectively). The methanolic extract (IC50 values of 2261 and 2046 μg/mL against CaCo2 and HeLa respectively) displayed only moderate anti-proliferative activity, demonstrating that antioxidant activity did not correspond with anti-proliferative activity. All of the extracts were determined to be nontoxic in the Artemia franciscana bioassay, with LC50 values substantially \>1000 μg/mL. Non-biased GC-MS headspace analysis of the C. maritima hexane extract highlighted several interesting compounds that may contribute to the therapeutic bioactivities of the extract. Conclusion: The lack of toxicity and the anti-proliferative activity of the hexane and ethyl acetate C. maritima extracts against HeLa and Caco2 cancer cell lines indicates their potential in the treatment and prevention of some cancers.

}, keywords = {Anticancer activity, Antioxidant, Brassicaceae, CaCo2, European searocket, HeLa, Oxidative stress}, doi = {10.5530/pj.2019.11.40}, author = {Elsayed Omer and Abdelsamed Elshamy and Rihab Taher and Walaa El-Kashak and Joseph Shalom and Alan White and Ian Cock} } @article {877, title = {The Combination of Spirulina Extract and Sambiloto Effect Histopathologically on Medial Colon from Plasmodium berghei Anka Infected Mice}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {May 2019}, pages = {564-569}, type = {Original Article}, chapter = {564}, abstract = {

Introduction: Malaria is one of the biggest burden in medical section in Indonesia, as the prevalence is still high and half of the provinces in Indonesia considered as endemic area.1-4 Moreover, the drug resistant case number has grown larger within years.3-7 Indonesian people are very close with traditional drug regiment that derived from plants, for example Sambiloto and Spirulina.7-9 This research done in order to see the effect of Sambiloto and Spirulina combination from histopathologic aspect in medial colon of P. berghei infected mice. Method: The data taken from experimental study using male Swiss Webster mice that has been infected with Plasmodium berghei Anka. The four groups of mice were given different treatment. The first group treated with Sambiloto only, the second one with Sambiloto and Spirulina extract, the third group with Sambiloto and Spirulina powder and the last one the control group with administration of DHP. Results: The result shows that the group with extract and powder spirulina show a significant result in the inflammatory focus and angiogenesis. However, this research does not necessarily prove the correlation between Sambiloto-Spirulina and their effect on the goblet cell and dysplasia grade on the infected mice, as the result for both category is insignificant. Conclusion: The study showed that spirulina has positive effect on inflamatory focus and angiogenesis, but the goblet cells count and dysplasia grade result is not noteworthy, as it requires prolonged inflammation process in order to achieve the optimal result.

}, keywords = {Malaria, Medial colon, Plasmodium berghei Anka, Sambiloto, Spirulina}, doi = {10.5530/pj.2019.11.90}, author = {Kusmardi Kusmardi and Rahyussalim and Rizky Priambodo Wisnubaroto and Dilla Firzani} } @article {1020, title = {Effect of Tender Coconut Water to Prevent Anemia on Wistar Rats Induced by Lead (Plumbum)}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1325-1330}, type = {Original Article}, chapter = {1325}, abstract = {

Background: Tender coconut water (TCW) is a nutritious healthy drink, some content as L-arginin, iron, vitamin C, vitamin B6, folic acid and fatty acid have roles during the process of blood formation (hematopoesis). Lead (Pb) is one of heavy metal which can trigger the formation of Reactive Oxygen Species (ROS), causing oxidative stress and hematopoetic stem cell disorders which cause the decrease of hematocrit, hemoglobin and erythrocyte levels as the sign of anemia. Aim and Objectives: This research to investigate the effect of tender coconut water to prevent anemia on lead induced rats. Material and Methods: Experimental research by post test only control group design, using 18 white wistar strain male rats randomly divided into 3 groups. Group K1 served as control, Group K2 was fed lead (10 mg/day/rat), Group K3 was given tender coconut water (8 mL/200gr BW rats/ day) orally for four weeks. Rats{\textquoteright} blood from ophtalmicus venous was analyzed using Hematology Analyzer to measure the hemoglobin, hematocrit, and erythrocyte levels. Data were analyzed using Anova test and Kruskal Wallis. Results:That decreased levels of hematocrit, hemoglobin and erythrocyte were show in group K2 compared with group K1. Increased levels of hematocrit, hemoglobin and erythrocyte were observed significantly in both groups K3 than K2. Conclusion: Results of the present study have administration of tender coconut water (8 mL/200gr BW rats/ day for 4 weeks) could increased of hematocrit, hemoglobin and erythrocyte levels (p \<0.05). The administration of tender coconut water has proven to prevent the anemia indicated by the increase of hematocrit, hemoglobin and erythrocyte levels on wistar strain male rats induced by lead.

}, keywords = {Anemia, Lead (Pb), Tender coconut water}, doi = {10.5530/pj.2019.11.204}, author = {Siti Thomas Zulaikhah and Joko Wahyuwibowo and Aziz Rakha D and Dede Rizky P and Ahmad Fauzi N} } @article {872, title = {Effectiveness of Methanol Solvent Extraction for Red Macroalgae Acanthophora spicifera Antitumoric Activity}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {May 2019}, pages = {450-454}, type = {Original Article}, chapter = {450}, abstract = {

Introduction: Red macroalgae Acanthophora spicifera is commonly found in most parts of Indonesia coastal areas. However, information regarding their potential pharmaceutical uses remains largely unexplored. This study evaluates the antiproliferative activity of Acanthophora spicifera collected from Indonesian extracted with different solvents of ethanol and methanol. Methods: Cytotoxicity and antiproliferative activity of A. spicifera crude ethanol extract (AS-EtOH) and A. spicifera crude methanol extract (AM-MetOH) were evaluated with Lactate dehidrogenase (LDH) and Trypan blue exclusion assay in Human cervical cancer (HeLa) cells for 72h. Concentration of extracts treated in HeLa cells were 6.25 to 200 μg/mL. Viability staining with fluorescence stain Hoechst33342/PI was conducted to investigate apoptotic activity. Potential apoptotic activity of treatments was confirmed by DNA fragmentation assay. Results: AS-MetOH demonstrated high cytotoxicity in HeLa cells with IC50 of 127.3 {\textpm} 33.13 μg/mL. However, AS-EtOH IC50 values could not be determined in this study. Antiproliferative activity was highly significant in AS-MetOH treated cells as cell density was suppressed to 20 x 104 cells/mL compared to AS-EtOH (73 x 104 cells/mL) and untreated cells (83 x 104 cells/mL). Finally, apoptotic activity could be determined in AS-MetOH treated cells with increase PI fluorescence emitting cells and DNA fragmentation. On the other hand, apoptotic activity was not observable in AS-EtOH treated cells based on fluorescence viability staining and DNA fragmentation assay. Conclusion: Current results show methanol solvent as an effective solvent to promote potential pharmaceutical properties of macroalgae A. spicifera. Further advanced studies in a compound level from A. spicifera methanol fraction would be reasonable for development of macroalgae based anticancer agent.

}, keywords = {Acanthophora, Antiproliferative, Cytotoxicity, Indonesia, Macroalgae}, doi = {10.5530/pj.2019.11.70}, author = {Eka Sunarwidhi Prasedya and Ni Wayan Riyani Martyasari and Candra Dwipayana Hamdin and Masao Miyake and Daisuke Kobayashi and Sri Widyastuti and Akihiro Hazama and Haji Sunarpi} } @article {899, title = {The Evaluation of Dietary Black Soybean and Purple Sweet Potato on Insulin Sensitivity in Streptozotocin - Induced Diabetic Rats}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {July 2019}, pages = {639-646}, type = {Original Article}, chapter = {639}, abstract = {

Introduction: Hyperglycemia mediates the production of excess free radicals and reduces endogenous antioxidant in type 2 diabetes mellitus (T2DM). Black soybean (BSB) is rich in antioxidant mainly from isoflavones, whereas the antioxidant of purple sweet potato (PSP) comes from high anthocyanin. The study aimed to evaluate the efficacy of BSB, PSP, and its combination on malondialdehyde (MDA), superoxide dismutase (SOD) concentrations, insulin and insulin receptor substrate-1 (IRS-1) expression in T2DM rats. Methods: T2DM induced by high-calorie diet for five weeks and then injected with a low dose of streptozotocin (30 mg/kg BW) intraperitoneally. The DM rats then treated with black soybean (DM + BSB), purple sweet potato (DM + PSP) and the combination of BSB and PSP 1:3, 2:2, 3:1 respectively (DM + C1- 3). Treatments were given for thirty days. The effect of BSB, PSP and its combination evaluated by measuring SOD and MDA, necrosis in pancreas evaluated through hematoxylin-eosin (HE) and insulin and IRS-1 expression in pancreas through immunohistochemistry-fluorescence (IHC-F). Results: Our result indicated that there were no significant differences of BSB and the combination in decreasing MDA concentrations. The ratio of BSB and PSP combination of 2:2 increase SOD towards near normal, decrease necrosis, and improve insulin and IRS-1. Conclusion: The combination of BSB and PSP had the potential to improve insulin sensitivity through the increase of SOD, reduce necrosis, and improve insulin and IRS-1 expression.

}, keywords = {Antioxidant, Malondialdehyde, Necrosis, Superoxide dismutase, T2DM}, doi = {10.5530/pj.2019.11.102}, author = {Abdul Gofur and Agung Witjoro and Erni Widya Ningtiyas and Evi Setyowati and Siti Aminatul Mukharromah and Mochammad Fitri Atho{\textquoteright}illah and Sri Rahayu Lestari} } @article {1017, title = {Free Radical Scavenging Capacity and Total Phenolic Contents in Peel and Fleshy Crude Extracts of Selected Vegetables}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1351-1358}, type = {Research Article}, chapter = {1351}, abstract = {

Context: Vegetables are sources of natural antioxidants which have beneficial health-promoting properties. Aims: This research was conducted to evaluate the antioxidant potentials and total phenolic contents in vegetable extracts. Settings and Design: The experiment was divided two groups using six vegetable peels and fleshes, including potato (Solanum tuberosum L.), carrot (Daucus carota L.), bitter gourd (Monordica Charantia L.), pumpkin (Cucurbita moschana Duchesn), Chinese radish (Raphanus sativus L.), and cucumber (Cucumis sativus L.). Materials and Methods: The samples were extracted with 95\% ethanol for 24 hrs and then analyzed the antioxidant activities by DPPH and FRAP assay and total phenolic contents was determined by Folin-Ciocalteu assay. Statistical analysis used: All the analysis was done in triplicate, and values are represented as means {\textpm} standard deviations. The data were subjected to one-way analysis of variance (ANOVA) and significance differences between mean values were determined by Tukey{\textquoteright}s multiple comparison tests (p \< 0.05). Pearson correlation coefficient was determined between the antioxidant activities and total phenolic contents using SPSS version 21. Results: The results revealed that antioxidant activities were different statistically significant in each group (p \< 0.05). Carrot peel was the most potent in DPPH radical scavenging activity (61.80 {\textpm} 6.14\%) and reducing power (0.75 {\textpm} 0.25 mM Fe2+/g extract) and potato flesh could inhibit DPPH radical (35.60 {\textpm} 0.63 \%) and had the highest FRAP value (0.61 {\textpm} 0.78 mM Fe2+/g extract). Pumpkin peel and Chinese radish flesh had the most total phenolic contents as 110.45 {\textpm} 22.49 and 142.15 {\textpm} 3.53 mg GAE/g extract, respectively. Although no correlations was between antioxidant activities and total phenolic contents in extracts but were significant correlations between DPPH and FRAP assay in vegetable peels and fleshes as 0.931 and 0.819, respectively. Conclusion: This study substantiates the potential of vegetable peels to be used as functional foods and natural free radical scavengers.

}, keywords = {Antioxidants, Free Radicals, Total Phenolic Contents, Vegetable fleshes, Vegetable peels}, doi = {10.5530/pj.2019.11.209}, author = {Chanate Wanna} } @article {996, title = {In silico Analysis of Flavonoid Glycosides and its Aglycones as Reverse Transcriptase Inhibitor}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1252-1255}, type = {Original Article}, chapter = {1252}, abstract = {

Background: HIV continues to be a major global public health issue, having claimed more than 35 million lives so far. In 2016, 1 million people died from HIV-related causes globally. HIV-1 reverse transcriptase is one of HIV{\textquoteright}s vital enzymes for virus reproduction. If the enzyme is inhibited, the virus multiplication could be significantly decreased. There are currently many treatments for HIV, but more effective treatment is always needed because of the possibility of drug resistance and side effects for long-term use. Based on the previous study, there are some natural compounds with high affinity to the HIV-1 reverse transcriptase enzyme. Some of these compounds are flavonoid glycosides. Aims and Method: This study was aimed to learn more about in silico HIV-1 reverse transcriptase inhibitory activities of flavonoid glycosides using docking method. Results: The results showed that the most recommended flavonoid glycosides are those with the lowest binding energy, which were kaempferol-3-O-rhamnoside, myricetin-3-O-rhamnoside and quercetin-3-O-rhamnoside. This was due to the interactions of all three flavonoid rings and sugar moiety with key amino acid residues, which were Leu100, Lys101, Glu138, Tyr181, His235 and Tyr318. Conclusion: Flavonoid glycosides with rhamnose as glycone showed lower binding energy on HIV-1 reverse transcriptase.

}, keywords = {Flavonoid, Glycosides, HIV, Molecular docking, Reverse transcriptase}, doi = {10.5530/pj.2019.11.194}, author = {Stefandi J Wijaya and Arry Yanuar and Rosita Handayani and Rezi Riadhi Syahdi} } @article {1050, title = {Influence of Extraction on the Yield, Phytochemical and LCMS Profile from Standardized Kemuning Leaf (Murraya paniculata (L.) Jack)}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {November 2019}, pages = {1455-1462}, type = {Original Article}, chapter = {1455}, abstract = {

Introduction: Mahanimbine alkaloid is carbazole alkaloids found in kemuning (Murraya paniculata (L.) Jack) potentially as antihyperlipidemia. Chemical compounds from plants can be obtained by extraction. The aim of this study was to determine influence of extraction solvents on the yield, phytochemistry and LCMS profiles from standardized kemuning to produce safe and quality medicinal raw materials. Materials and Methods: The study was used kinds of ethanol concentration with different polarity as a solvent for extraction. Solvent 40\%, 70\% and 96\% of ethanol were used for maceration of simplicia from Bogor, West Java. The extract obtained was evaluated for the content of alkaloid compounds for LCMS then extracts were selected to be proceed to standardized extract quality. Results: The LCMS results showed that all 40\%, 70\% and 96\% ethanol extracts contained Candidate Mass 354,19014 compounds which were thought to be e alkaloids based on the m/z value. Phytochemical screening in all extracts contained alkaloid compounds, flavonoids, saponins, tannins and steroids/triterpenoids. 96\% ethanolic extract contained non-specific parameter such as content of compounds soluble in water 22,73\%, soluble in ethanol 17,37\%, drying down 9,10\%, water content 18,36\%, total ash content 4,18\%, the yield of extracts was 33,45\%, microbial contamination \<3,0.104 (7,0.104) colony/g and mold yeast number 0 colony/g. The total gravimetric alkaloid level was 1,031\%. Conclusions: 96\% ethanolic extract of kemuning contains mahanimbine alkaloids and has met the standard quality requirements of extracts.

}, keywords = {Murraya paniculata, Non-specific parameters, Pharmacognosy, Specific parameters, Standardization of extracts}, doi = {10.5530/pj.2019.11.225}, author = {Elly Wardani and Yahdiana Harahap and Abdul Mun{\textquoteright}im and Anton Bahtiar} } @article {901, title = {Liquid Chromatography Single Quadrupole Mass Spectrometry (LC/SQ MS) Analysis Reveals Presence of Novel Antineoplastic Metabolites in Ethanolic Extracts of Fruits and Leaves of Annona muricata}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {July 2019}, pages = {660-668}, type = {Original Article}, chapter = {660}, abstract = {

Background: Annona muricata, a tropical plant species belonging to family Annonaceae is one of the most used plants in folk medicine because of its many medicinal uses. Despite its wide usage, there is still need to continue scientifically evaluating its medicinal properties in order to avoid any adverse effects. Elucidating the detailed chemical composition of this plant is a significant step towards this evaluation. Objective: The aim of this study was to conduct LC MS analysis on the ethanolic extracts of fruits and leaves of Annona muricata for detection of novel metabolites. Materials and Methods: Leaves and fruits of Annona muricata were collected from Eastern Uganda during the month January 2018. Extraction was conducted using the tissue homogenization method and the extracts were analyzed on an LC/SQ MS detection system. The results were obtained by analyzing the MS spectra using the retentions time and fragmentation patterns on the NIST Library. Results: The study revealed that the fruits extracts contain 1,3-Dimethylthiourea and (4-chlorophenyl)-[4-(3-chlorophenyl)-2-[(Z)-3-(dimethylamino) prop-1-enyl]quinolin-6-yl]-(3-methylimidazol-4-yl)methanol, which are reported antioxidant and antineoplastic agents. The leaves contained 2,4,6-Tribromoaniline another antioxidant and antineoplastic agent, while compound (dichlorozirconium(2+);dimethyl-bis(2-methyl-4- phenylinden-1-id-1-yl)silane was found in both extracts of fruits and leaves. Conclusion: The current study suggests that ethanolic extracts of fruits and leaves of Annona muricata contain compounds which are potent antioxidant, antineoplastic and therapeutic agents for various conditions and paves the way for the development of several treatment regimens from these plant parts. Finally, the compounds reported in this study have been identified for the first time as being found in Annona muricata.

}, keywords = {Annona muricata, Antineoplastic, Antioxidant, Ethanolic extracts, LC/SQ MS, Phytochemicals}, doi = {10.5530/pj.2019.11.104}, author = {Yahaya Gavamukulya and Esther N Maina and Amos M Meroka and Edwin S Madivoli and Hany A El-Shemy and Gabriel Magoma and Fred Wamunyokoli} } @article {832, title = {Phytochemical Constituents and Free Radical Scavenging Activity of Madang Gatal (Schima wallichii) Choisy Stem Bark}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {February 2019}, pages = {395-399}, type = {Original Article}, chapter = {395}, abstract = {

Introduction: Madang gatal (Schima wallichii) Choisy is one of typical Indonesian plants that used traditionally as insomnia and hypertension drug. Background: The environment is highly polluted by free radicals which may contribute to the aging process of tissue and cause chronic diseases. The aims of the research were to study the phytochemical constituents and free radical scavenging activity of Schima wallichii stem bark. Materials and Methods: Extraction of S.wallichii stem bark was carried out by maceration method using methanol and then the methanol extract was partitioned using n-hexane, ethyl acetate and butanol successively. The methanol extract was then screened to determine the presence of different classes of secondary metabolites. The ethyl acetate fraction was subjected to silica column chromatography followed by exclusion chromatography on sephadex LH-20 to yield two partially purified isolates, SA and SE4. FTIR and GC-MS analyses indicated SA asa mixture of pentadecanoic acid, 14-methyl-, methyl esterand patchouli alcohol, while SE4 was made up predominantly of pentadecanoic acid, 14-methyl-, 13-hydroxy methyl ester. Free radical scavenging activity was performed by using2,2-diphenyl-1-picrylhydrazyl (DPPH) radical oxidation method. Results: The phytochemical screening indicated the presence of alkaloids, flavonoids, tannins, saponins, terpenes and quinones. All tested samples, methanol, n-hexane, ethyl acetate and butanol extracts showed strong antioxidant activity with IC50 values of 8.58, 17.36, 8.17 and 8.79 μg/mL, respectively. The isolates showed strong antioxidant activity with SE4 exhibiting an IC50 value of about 5.5 times stronger than quercetin used as a standard antioxidant.

}, keywords = {13-hydroxy methyl ester, 14-methyl-, antioxidant activity, Chromatography, DPPH, Pentadecanoic acid, Schima wallichii stem bark}, doi = {10.5530/pj.2019.11.61}, author = {Galuh Widiyarti and Winda Fitrianingsih} } @article {918, title = {Screening of Tyrosinase Inhibitor, Antioxidant and Cytotoxicity of Dried Sea Cucumber from Tomini Bay, Indonesia}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {May 2019}, pages = {555-558}, type = {Original Article}, chapter = {555}, abstract = {

Background: Indonesia, as a tropical country, is one of the important producers of sea cucumbers (beche-de-mer). Sea cucumber is a marine invertebrate that contains attractive bioactive secondary metabolites and these metabolites can be used for health as well as cosmetics. Objective: The aim of the study was to determine the activity of tyrosinase inhibitors, antioxidants, and cytotoxicity of sea cucumber methanolic extract. Methods: Dried sea cucumber samples were taken from Boalemo waters, Tomini Bay, Indonesia. Tyrosinase inhibitor assay was carried out spectrophotometrically using tyrosinase enzymes and L-DOPA as a substrate and antioxidant tests were carried out by DPPH method. Cytotoxicity test against human breast cancer cell line (T47D) was conducted using the MTT assay. Results: The study showed that Bohadschia vitiensis had the best tyrosinase inhibitor activity with IC50 value of 0.28 mg/ml. The DPPH free radical scavenging testing showed that all sea cucumbers had weak antioxidant activity. On the other hand, cytotoxicity assay revealed that several sea cucumbers had good cytotoxicity against T47D cells, where Holothuria atra and Bohadschia marmorata showed strong cytotoxicities with IC50 values of 23.0 and 28.1 ug/mL, respectively. Conclusion: Based on the study, it can be concluded that the dried sea cucumber from the Tomini bay region, Indonesia, has the potential to be developed as a source of tyrosinase inhibitors and cytotoxic agents.

}, keywords = {Antioxidant, Cytotoxicity, Screening, Sea cucumber, Tyrosinase inhibitor}, doi = {10.5530/pj.2019.11.88}, author = {Muhammad Nursid and Endar Marraskuranto and Azizah Kuswardini and Tjahyo Winanto} } @article {842, title = {Xanthones Analysis and Antioxidant Activity Analysis (Applying ESR) of Six Different Maturity Levels of Mangosteen Rind Extract (Garcinia mangostana Linn.)}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {March 2019}, pages = {369-373}, type = {Original Article}, chapter = {369}, abstract = {

Background: Mangosteen (Garcinia mangostana Linn.) is tropical fruit that known to contain bioactive compounds which has particular function as antioxidants, anti-inflammatory and antibacterial. Mangosteen rind extract is known to have high contents of xanthone compounds which have antioxidant and free radical scavenging activities. This study was aiming to determine the contents of xanthone derivatives using Liquid Chromatography Mass Spectrometry (LC-MS/MS) and antioxidant activity of six different maturity levels of mangosteen rind extract using electron spin resonance (ESR). Method: Mangosteen was classified based on the maturity level by its visual characteristic and divided into six criteria of maturity. The mangosteen rinds were extracted through maceration and freeze drying method. Qualitative analysis of xanthone was obtained using LC-MS/MS. Antioxidant activity was determined using ESR spectrometer. To evaluate antioxidant activity, using 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used as free radical scavenger method. Results: The LC-MS/MS analysis showed that the highest concentrations are filled by mangosteen rind with maturity level 6. Moreover, each maturity levels of mangosteen rind extract has different g value compared to DPPH free radical. Different g value indicated the different type of free radical and decreasing g value indicated the declining intensity of free radical. The higher decreasing of g value was mangosteen rind with maturity level 4, 5 and 6. Conclusion: Mangosteen rind extract contains xanthone and its derivatives and ESR analysis showed that different maturity levels on mangosteen rind has different antioxidant activity as indicated by declining free radical intensity.

}, keywords = {Antioxidant, Electron spin resonance (ESR), Free radical, Mangosteen rind extract, Xanthone Garcinia mangostana}, doi = {10.5530/pj.2019.11.56}, author = {Marisca Evalina Gondokesumo and Bambang Pardjianto and Sutiman Bambang Sumitro and Wahyu Widowati} } @article {643, title = {Alpha-Mangostin Effect on Inhibition Development Stadium and Globin Accumulation Against Plasmodium falciparum}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {783-788}, type = {Original Article}, chapter = {783}, abstract = {

\α--Mangostin is a widely reported group of Xanthone compounds from the Clusiaceae family of 40 genera and over 1000 species spread across the tropics and subtropics area. The Objective for determine effect of \α-mangostin from Garcinia tetrandra Pierre stem bark against development stadium inhibition and globin accumulation of Plasmodium falciparum. Inhibition stadium development assay used based on the Rosenthal method. Plasmodium falciparum parasitic globin accumulation assay. Globin accumulation assay used the highest concentration of in vitro antimalarial test using SDS-PAGE with positive control E-64 and \α-mangostin were incubated together with the malaria parasite during 24 h. Result of Inhibition stadium development of Plasmodium falciparum against \α-mangostin show inhibition from development stadium of the malaria parasite Plasmodium falciparum, Electrophoresis show globin accumulation from electrophoresis followed by staining using Coomassie brilliant blue. \α-mangostin showed inhibition the growth of malaria parasite Plasmodium falciparum with dose 10 \μg/ml is expected to occur a large accumulation of globin, which can be viewed both morphologically and by the method of SDS-PAGE.

}, keywords = {Development stage inhibition, Garcinia tetrandra, Plasmodium falciparum, SDS-PAGE, α-mangostin}, doi = {10.5530/pj.2018.4.132}, url = {http://fulltxt.org/article/670}, author = {Hadi Kuncoro and Aty Widyawaruyanti and Taslim Ersam} } @article {770, title = {Antioxidant Activity, Total Phenolic and Flavonoid Content of Berberis aristata DC. and Berberis thomsoniana C.K. Schneid. from Sagarmatha National Park, Nepal}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {November 2018}, pages = {s167-s171}, type = {Original Article}, chapter = {s167}, abstract = {

Background: Medicinal plants are the major sources of the crude drugs and natural antioxidants. Berberis species have been used in the treatment of various ailments around the world including Nepal. However, systematic study on phytochemistry and pharmacology of Berberis species from Nepal is lacking. Objectives: To evaluate the antioxidant activity, total phenolic and flavonoid contents of Berberis aristata and Berberis thomsoniana from Sagarmatha National Park. Materials and Methods: Antioxidant activity was measured through 2, 2-Diphenyl-1-Picrylhydrazyl assay. Total phenolic and flavonoid content were estimated using Folin\–Ciocalteau and aluminum chloride method respectively. Results: Samples showed dose-dependent radical scavenging activity. Radical scavenging activity of the methanolic extracts of different parts of B. aristata and B. thomsoniana ranged from 19.38 to 98.47\%, with leaf extracts of B. thomsoniana showing the strongest activity. The total phenolic content of the samples varied from 11.04 to 65.30 mg GAE g-1 dry weight whereas total flavonoid content was in between 2.4 to 16.46 mg quercetin/g dry weight. Conclusion: Among the tested samples, leaf extracts of B. thomsoniana showed the strongest antioxidant activity and contained the highest amount of total phenolic and flavonoid content.

}, keywords = {antioxidant activity, Berberis, Flavonoid content, Nepal, Phenolic content}, doi = {10.5530/pj.2018.6s.29}, author = {Lok Ranjan Bhatt and Bina Wagle and Minu Adhikari and Santoshi Bhusal and Anjana Giri and Shandesh Bhattarai} } @article {736, title = {Arginase Inhibitory and Antioxidant Activities of Caesalpinia coriaria (Jacq.) Willd. Bark Extract}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1174-1179}, type = {Original Article}, chapter = {1174}, abstract = {

Objective: The aim of this study was to investigate the arginase inhibitory and the antioxidant activities of the bark extract of Caesalpinia coriaria (Jacq.) Willd. (Dewi tree). Methods: The bark of Dewi tree was extracted successively under reflux condition with n-hexane, ethyl acetate, and methanol. Each extract was tested for its activity in inhibiting arginase activity by measuring the quantity of urea produced in the reaction mixture using a microplate reader. The active extracts were determined for their total flavonoid content followed by antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted to determine the presence of alkaloids, saponins, flavonoids, tannins, and steroids. Results: Arginase inhibitory activity test showed that the ethyl acetate and methanol extracts have average inhibition values of 14.43 and 33.59\%, respectively, at concentration of 100 \μg/mL. The total flavonoid content of the methanol and ethyl acetate extract were 7.75 and 6.30 mgQE/g sample, respectively. The methanol and ethyl acetate extracts showed antioxidant activity with an IC50 values of 4.720 and 3.647 \μg/mL, respectively. The ethyl acetate extract contained flavonoid, tannin, saponin, and steroid, while the methanol extract contained flavonoid, tannin, and saponin. Conclusion: In conclusion, C. coriaria bark extracts possessed low arginase inhibitory activity. The methanol and ethyl acetate extracts have good antioxidant activity.

}, keywords = {antioxidant activity, Arginase inhibitory activity, C. coriaria, Phytochemicalscreening, total flavonoid content}, doi = {:10.5530/pj.2018.6.201}, author = {Arini Wulansari and Berna Elya and Arikadia Noviani} } @article {730, title = {Cytotoxic Activity of Ethanol Extract of Arbuscular Mycorrhizal Fungi Induced Ginger Rhizome on T47D Breast Cancer Cell Lines}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1133-1136}, type = {Original Article}, chapter = {1133}, abstract = {

Objective: A study of investigate the cytotoxicity activity of ethanolic extract of ginger (Zingiber officinale Rosc.) induced with arbuscular mycorrhizal fungi (AMF) against T47D cells line breast cancer have been conducted. Methods: Cytotoxicity were determined using the \“microtetrazolium (MTT) Assay\”, by measuring the activity of mitochondrial dehydrogenase in living cells that have ability to convert pale yellow of dissolved MTT to purple formazan product. The extract used at various concentration (0.1, 1.0, 10 and 100 \μg / mL. The level of cytotoxic actifity was determined by calculating the inhibitory concentration (IC50) value that was based on the precentage of cell death after 24 h treatment with the extract. The change of cell morphology were observed by using inverted microscope. Results: The statistic results proved that ethanol extract of AMF induced ginger rhizome could barriers T47D breast cancers significantly at concentrations of 10 \μg / mL and 100 ug / mL, with IC50 value was 12.5 \± 3.73 \μg / mL. centration of 0.1 \μg / mL, 1.0 \μg / mL, 10 \μg / mL and 100 mg / mL. Results of statistical analysis showed that the ethanol extract of ginger rhizome induced AMF at a concentration of 10 \μg / mL and 100 \μg / mL was able to inhibit the growth of breast cancer cells T47D significantly. Conclusion: The results showed the ethanol extract of AMF induced ginger rhizome was potential as herbal medicine for cancer-related ailments with IC50 value was 12.5 \± 3.73 \μg / mL.

}, keywords = {AMF, Breast cancer, Cytotoxicity, Ginger, MTT Assay, T47D}, doi = {xx10.5530/pj.2018.6.193}, author = {Netty Suharty and Fatma Sri Wahyuni and Dachriyanus} } @article {655, title = {The Effect of Coconut Water and Boiling on Antioxidant Activity and Total Phenolic Contents in Schizophyllum commune Fr.}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {July/2018}, pages = {73-77}, type = {Original Article}, chapter = {73}, abstract = {

Context: Schizophyllum commune Fr., a split gill mushroom has been acknowledged for its medicinal properties. Aims: The present study was to evaluate the effect of coconut water and boiling on antioxidant activity and total phenolic contents in Schizophyllum commune Fr. Settings and Design: The experiment was completely randomized design (CRD) using tender and mature coconut water compared to synthetic hormone at concentration of 50\% and 100\% in volume of 5 and 10 ml. The first group is fresh mushrooms and the second group is boiled mushroom then was dried in hot air oven at 45\°C for 48 hr. Materials and Methods: The S. commune was extracted with ethanol and then was evaluated the scavenging activity by DPPH and FRAP assay and Folin-Ciocalteau method was used to determine total phenolic contents. Statistical analysis used: The data were recorded as means \± standard deviations and analyzed by using SPSS version 21. The mean values of data were subjected to a oneway analysis of variance (ANOVA) and the significance of the difference between means was determined by the Tukey\’s multiple comparison tests at 95\% least significance difference (p \< 0.05). Pearson correlation coefficient was used to determine the relationship between the antioxidant activity and total phenolic contents in sample extracts. Results: In fresh mushroom group showed higher antioxidant activity and total phenolic contents than boiled mushroom group. When compared to a loss of antioxidant activity and total phenolic contents after boiling only the experiments with coconut water found that tender coconut water 50\% of 5 ml has the lowest of loss in TEAC and total phenolic contents as 9.28\% and 9.85\% respectively. Mature coconut water 100\% of 10 ml has the lowest of loss in FRAP as 2.77\% and followed by tender coconut water 50\% of 5 ml as 4.82\% which were lower than control treatment but similar with synthetic hormone of 5 ml. The relationship between antioxidant activity and total phenolic contents of mushrooms was low in both groups. Conclusions: It concluded that tender coconut water 50\% of 5 ml could maintain the antioxidant properties and total phenolic compounds in S. commune.

Key-words: Antioxidant, Free radicals, Total phenolic contents, Coconut water, Schizophyllum commune Fr.

Key Messages: The tender coconut water 50\% of 5 ml was suitable for holding the antioxidant properties and total phenolic contents and also boiling affect to loss of antioxidant compounds in S. commune. The findings from this study provide information for alternative selection of coconut water as natural hormone.

}, doi = {10.5530/pj.2017.3.14}, author = {Chanate Wanna and Montarop Sudhadham} } @article {676, title = {The Effect of Coconut Water and Boiling on Antioxidant Activity and Total Phenolic Contents in Schizophyllum commune Fr.}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {925-931}, type = {Original Article}, chapter = {925}, abstract = {

Context: Schizophyllum commune Fr., a split gill mushroom has been acknowledged for its medicinal properties.\  Aims: The present study was to evaluate the effect of coconut water and boiling on antioxidant activity and total phenolic contents in Schizophyllum commune Fr. Settings and Design: The experiment was completely randomized design (CRD) using tender and mature coconut water compared to synthetic hormone at concentration of 50\% and 100\% in volume of 5 and 10 ml. The first group is fresh mushrooms and the second group is boiled mushroom then was dried in hot air oven at 45\°C for 48 hr. Materials and Methods: The S. commune was extracted with ethanol and then was evaluated the scavenging activity by DPPH and FRAP assay and Folin-Ciocalteau method was used to determine total phenolic contents. Statistical analysis used: The data were recorded as means \± standard deviations and analyzed by using SPSS version 21. The mean values of data were subjected to a oneway analysis of variance (ANOVA) and the significance of the difference between means was determined by the Tukey\’s multiple comparison tests at 95\% least significance difference (p \< 0.05). Pearson correlation coefficient was used to determine the relationship between the antioxidant activity and total phenolic contents in sample extracts. Results: In fresh mushroom group showed higher antioxidant activity and total phenolic contents than boiled mushroom group. When compared to a loss of antioxidant activity and total phenolic contents after boiling only the experiments with coconut water found that tender coconut water 50\% of 5 ml has the lowest of loss in TEAC and total phenolic contents as 9.28\% and 9.85\% respectively. Mature coconut water 100\% of 10 ml has the lowest of loss in FRAP as 2.77\% and followed by tender coconut water 50\% of 5 ml as 4.82\% which were lower than control treatment but similar with synthetic hormone of 5 ml. The relationship between antioxidant activity and total phenolic contents of mushrooms was low in both groups. Conclusions: It concluded that tender coconut water 50\% of 5 ml could maintain the antioxidant properties and total phenolic compounds in S. commune.\ 

}, keywords = {Antioxidant, Coconut water, Free Radicals, Schizophyllum commune Fr, Total Phenolic Contents}, doi = {10.5530/pj.2018.5.156}, author = {Chanate Wanna and Montarop Sudhadham} } @article {549, title = {High Efficiency in vitro Plant Regeneration and Secondary Metabolite Quantification from Leaf Explants of Rhodiola imbricata}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {x}, pages = {x}, type = {x}, chapter = {XX}, abstract = {

Aim: Rhodiola imbricata is an endangered medicinal plant of the trans-Himalayan Leh-Ladakh region belonging to the family Crassulaceae. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant and evaluation of cinnamyl alcohol (Secondary metabolite) was established in this study. Material and Methods: In vitro grown leaves were inoculated using Murashige and Skoog (MS) medium supplemented with (alpha)- naphtalene acetic acid (NAA) in combination with 6- benzyladenine (BAP) for callus proliferation and regeneration. Results: The highest percentage of rhizogenous callus was induced in medium containing NAA (10.0-15.0 M). The highest percentage of shoot formation from leaf derived callus was obtained in the medium containing of NAA (5.0 M) and BAP (2.5 M) as well as in NAA (1.0 M), BAP (5.0 M) (38.88\% and 37.49\%) respectively. Rooting of regenerated shoots were effective when a lower concentration of NAA (0.5 M) was used alone. A maximum number of roots (22.0) and higher length (0.6 cm) was observed. The in-vitro plantlets with well-developed shoots and roots were acclimatized successfully to natural field conditions with a survival rate of over 80\%. Cinnamyl alcohol (Secondary metabolite) evaluation was also done for the very first time and an upregulation of 49.6\% and 30.6\% were observed in in-vitro roots and shoots when compared with mother root and shoot respectively. Hence, it was proved that the content of secondary metabolites obtained from in-vitro raised plants is higher than mother plant. Conclusion: These results may lay a foundation for genetic improvement and can be used to determine sequential level of targeted secondary metabolites through cell culture in Rhodiola imbricata.

Key words: Regeneration system, Acclimatization, Endangered, Plant growth regulator, Cinnamyl alcohol.

}, keywords = {Acclimatization, Cinnamyl alcohol., Endangered, Plant growth regulator, Regeneration system}, doi = {10.5530/jppcm.2018.2s.xx}, author = {Ashwani Kumar Bhardwaj and Avilekh Naryal and Pushpender Bhardwaj and Ashish Rambhau Warghat and Balpreet Arora and Shikha Dhiman and Shweta Saxena and Pratap Kumar Pati and Om Prakash Chaurasia} } @article {582, title = {High Efficiency in vitro Plant Regeneration and Secondary Metabolite Quantification from Leaf Explants of Rhodiola imbricata}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {470-475}, type = {Original Article}, chapter = {470}, abstract = {

Aim: Rhodiola imbricata is an endangered medicinal plant of the trans-Himalayan Leh-Ladakh region belonging to the family Crassulaceae. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant and evaluation of cinnamyl alcohol (Secondary metabolite) was established in this study. Material and Methods: In vitro grown leaves were inoculated using Murashige and Skoog (MS) medium supplemented with (alpha)- naphtalene acetic acid (NAA) in combination with 6- benzyladenine (BAP) for callus proliferation and regeneration. Results: The highest percentage of rhizogenous callus was induced in medium containing NAA (10.0-15.0 M). The highest percentage of shoot formation from leaf derived callus was obtained in the medium containing of NAA (5.0 M) and BAP (2.5 M) as well as in NAA (1.0 \μM), BAP (5.0 M) (38.88\% and 37.49\%) respectively. Rooting of regenerated shoots were effective when a lower concentration of NAA (0.5 M) was used alone. A maximum number of roots (22.0) and higher length (0.6 cm) was observed. The in-vitro plantlets with well-developed shoots and roots were acclimatized successfully to natural field conditions with a survival rate of over 80\%. Cinnamyl alcohol (Secondary metabolite) evaluation was also done for the very first time and an upregulation of 49.6\% and 30.6\% were observed in in-vitro roots and shoots when compared with mother root and shoot respectively. Hence, it was proved that the content of secondary metabolites obtained from in-vitro raised plants is higher than mother plant. Conclusion: These results may lay a foundation for genetic improvement and can be used to determine sequential level of targeted secondary metabolites through cell culture in Rhodiola imbricata.

}, keywords = {Acclimatization, Cinnamyl alcohol, Endangered, Plant growth regulator, Regeneration system}, doi = {10.5530/pj.2018.3.77}, url = {http://fulltxt.org/article/510}, author = {Ashwani Kumar Bhardwaj and Avilekh Naryal and Pushpender Bhardwaj and Ashish Rambhau Warghat and Balpreet Arora and Shikha Dhiman and Shweta Saxena and Pratap Kumar Pati and Om Prakash Chaurasia} } @article {713, title = {Immunostimulatory Activity of Chitosan Nanoparticles on Wistar Albino Rats}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {892-898}, type = {Original Article}, chapter = {892}, abstract = {

Background: The active components of natural products provide a potential alternative to conventional immunotherapy for a variety of diseases conditions and become subject to scientific investigations currently worldwide. Objective: The purpose of this research was to investigate the immunostimulatory activity of the chitosan nanoparticle on Wistar albino rats. Materials and Methods: The present investigation was carried out on various groups of healthy adult rats. The assessment of immunomodulatory potential was carried out by neutrophil adhesion test, delayed-type hypersensitivity (DTH) response, haemagglutinating antibody (HA) titre, cyclophosphamide-induced myelosuppression and phagocytic activity were determined in various groups of animals. Results: The administration of chitosan nanoparticle at doses 300 mg/kg BW and 600 mg/kg BW but not at doses 150 mg/kg BW significantly increased in neutrophil adhesion fibers, haemagglutinating antibody titre values and potentiated the inhibited type hypersensitivity reaction induced by sheep red blood cells. Also, it had good response towards phagocytosis in carbon clearance assay and prevented myelosuppression of cyclophosphamide on rats. Conclusion: From these findings, it can be concluded that chitosan nanoparticle responsible for immunostimulatory activity and has therapeutic potential for the prevention of immune depressed conditions.

}, keywords = {Chitosan nanoparticle, Delayed-Type Hypersensitivity, Haemagglutinating antibody, Myelosuppression, Neutrophil adhesion, Phagocytosis}, doi = {10.5530/pj.2018.5.150}, author = {Giftania Wardani and Mahmiah and Sri Agus Sudjarwo} } @article {625, title = {In silico, in vitro and in vivo Tests of Ficus deltoidea Jack Leaves Extract as Inhibitor for Beta-Catenin Expression in Colon Carcinogenesis Model}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {808-813}, type = {Original Article}, chapter = {808}, abstract = {

Context: Ficus deltoidea Jack leaves extract as anticolorectal cancer. Aims: This study aims to analyze the potential of FD extract to be an anti-colon cancer by investigating the extract capability in reducing \β-catenin expression and inhibiting colon cancer cells growth. Settings |and Design: The research was conducted in Medical Faculty Universitas Indonesia with experimental design. Methods and Material: FD ethanol extracts was tested in vitro, in silico and in vivo. In vitro test was conducted to human colon cell lines. In vivo test was conducted to Balb/c mice induced with 10 mg/kg azoxymethane (AOM) and dextran sodium sulfate 1\% (DSS). The colonic tissue collected was the distal portion. \β-catenin expressions in the cytoplasm and nuclei of the epithelial cells of the colon crypt were semi quantitatively assessed using the immunohistochemistry staining on ten visual fields with 400x magnification. Statistical analysis used: SPSS. Results: FD ethanol extracts inhibit the expression of \β-catenin in the crypt ephitelial cells of mice colon induced with AOM/DSS. The extracts also inhibit the growth of human colon cancer (HCT 116) with IC50 value of 5.41 mg/mL. Phytochemical screening to the extracts gave three groups of compounds: alkaloid, flavonoid, and tannin. Water fraction is the best fraction. Based on in the results of in silico analysis with molecular docking, FD extract is believed to influence the expression of \β-catenin, in which vitexin and isovitexin are the main candidate compounds to influence the expression of the protein. Conclusion: FD ethanol extract is potential to be an anti-colon cancer proven by the extract capability to reduce \β-catenin expression.

}, keywords = {Azoxymethane, Colon carcinogenesis, Ficus deltoidea, in silico, β-catenin}, doi = {10.5530/pj.2018.4.137}, url = {http://fulltxt.org/article/675}, author = {Kusmardi Kusmardi and Tedjo Aryo and Wuyung Puspita Eka and Fadilah Fadilah and Priosoeryanto Bambang Pontjo and Fachri Wilzar} } @article {488, title = {In vitro Antibacterial Activity of Chitosan Nanoparticles against Mycobacterium tuberculosis}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {December 2017}, pages = {162-166}, type = {Original Article}, chapter = {162}, abstract = {

Background: Chitosan nanoparticles have gained growing interest for nanomedicine, biomedical engineering and development of new therapeutic with improved bioavailability, increased sensitivity and specificity, and reduced toxicity. Objective: The aim of the present study is to synthesis of the chitosan nanoparticles for antimycobacterial applications. Methods: Chitosan were isolated from the shrimp shell. Tripolyphosphate (TPP) will be used to prepare chitosan nanoparticles by ionotropic gelation method. The size and morphology of the chitosan nanoparticle was analyzed by scanning electron microscope (SEM). The broth microdilution method is used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of nanoparticle chitosan on strain M. tuberculosis H37Rv. Results: The SEM micrographs of the nano-magnetic chitosan showed that they were approximately uniform spheres and the rough surface morphology, have a solid dense cubical or rectangular structure. Using the broth microdilution susceptibility method, chitosan nanoparticle was found to have the antimycobacterial effects with a MIC value of 1200 \μg/mL whilst MBCs value of 2400 \μg/mL for M. tuberculosis H37Rv. Conclusion: The conclusion from the study was chitosan nanoparticle have potential as a source of lead compounds that may be developed further into antimycobacterial drugs.

}, keywords = {Chitosan nanoparticle, M. tuberculosis H37Rv, Minimal bactericidal concentration., Minimal inhibitory Concentration}, doi = {10.5530/pj.2018.1.27}, url = {http://fulltxt.org/article/413}, author = {Giftania Wardani and Mahmiah and Sri Agus Sudjarwo} } @article {689, title = {In vitro Assay of Alpha-Glucosidase Inhibitor Activities of Three Seagrasses from Banten Bay, Indonesia}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {907-910}, type = {Original Article}, chapter = {907}, abstract = {

Introduction: Diabetes mellitus (DM) is an endocrine disorder characterized by high blood glucose levels. One of the approaches for treating DM is by regulating the postprandial blood glucose via inhibition of \α-glucosidase enzymes. Seagrass is known as a plant containing bioactive compounds, especially for therapy antidiabetic. This research aims to evaluate the \α-glucosidase inhibition activity of three species seagrasses (Enhalus acoroides, Thalassia hemprichii and Cymodocea rotundata) from the Banten Bay, Indonesia. Methods: The extracts of all parts (root, leaf, and stem) from three seagrasses were prepared with maceration method using 80\% ethanol solvents. The \α-glucosidase inhibitory activity was conducted by microplate reader at 400 nm using acarbose as a positive control. Furthermore, the kinetics of \α-glucosidase inhibition, phytochemical screening, and total phenolics content were evaluated against extract which has the most potential \α-glucosidase inhibition. Results: The result showed that the 80\% ethanol extracts of Enhalus acoroides (IC50 values 168.15 \± 2.71 g/mL) had the most potential \α-glucosidase inhibitors activity compared with the positive control acarbose, Thalassia hemprichii and Cymodocea rotundata (IC50 values 197.27 \± 3.07 g/mL, 425.86 \± 5.15 g/mL and 429.28 \± 8.89 g/mL). The kinetic type of inhibition against \α-glucosidase was noncompetitive inhibition. The phytochemical compounds were phenols, flavonoid, terpenes, and tannin with the total phenolic content was 28.76 \± 2.46 mgGAE/g. Conclusion: Enhalus acoroides has the strongest inhibitor of \α-glucosidase and can be further developed for DM therapy agents.

}, keywords = {Cymodocea rotundata, Diabetes mellitus, Enhalus acoroides, Phytochemical compound, Thalassia hemprichii}, doi = {10.5530/pj.2018.5.152}, author = {Agung Widiyanto and Effionora Anwar and Tati Nurhayati} } @article {729, title = {Network Analysis of Indigenous Indonesia Medical Plants for Treating Tuberculosis}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1159-1164}, type = {Original Article}, chapter = {1159}, abstract = {

Background: Indonesia is the biggest archipelago country with the second biggest biodiversity in the world. A lot of medical plants for treating various diseases can be found in Indonesia, including medical plants for tuberculosis, an infectious disease caused by Mycobacterium tuberculosis. Objective: The goal of this research is to document the information of Indonesia indigenous medical plants that used various local societies to treat tuberculosis and also analyze active compounds of medical plants with proteins that related to tuberculosis. Methods and Material: The annotation of medical plants for treating tuberculosis was collected from a various source comprising local research papers, theses, and other resources. The information of active compound was taken from Dr. Duke\’s Phytochemical and Ethnobotanical Databases. A network of active compounds-proteins was analyzed by using Cytoscape 3.6.0. Results: The result described that there were twenty-seven species from nineteen families of medical plants used by local societies of Indonesia for tuberculosis therapy, and there were sundry of active compounds from fourteen medical plants had direct interaction with proteins related tuberculosis. Conclusions: Most of the active compounds targeted proteins that had a prominent role in immune system. It indicated that medical plants treating tuberculosis through regulating immunity of human body.

}, keywords = {Cytoscape, Immune system, Indonesia medical plants, Network, Tuberculosis}, doi = {xx10.5530/pj.2018.6.198}, author = {Sherry Aristyani and Sri Widyarti and Sutiman Bambang Sumitro} } @article {489, title = {Phytochemical Screening, Antibacterial Activity, and Mode of Action on Morus nigra}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {December 2017}, pages = {167-171}, type = {Original Article}, chapter = {167}, abstract = {

Introduction: The Morus nigra (Moraceae) known as black mulberry is a well-grown plant in Lembang, Indonesia. Previous studies showed that black mulberry fruit has activity as antibacterial and antioxidant. The aims of this study were to evaluate the antibacterial effect of ethanol extract from stem bark, fruit, and leaves of Morus nigra, MIC and morphological changes of the most active extract against Staphylococcus epidermidis and Propionibacterium acnes. Methods: Agar diffusion and microdilution assays were used to evaluate the antibacterial effect of the extract by measuring the zone of inhibition and the minimum inhibitory concentration (MIC) of the extract against the test bacteria. Scanning electron microscopy (SEM) was performed to evaluate the morphological changes induced by the extract in cellular membrane of the bacteria. Protein and ion leakage from the bacterial cells induced by the extract were also evaluated. Results: The antibacterial activity showed the most active extract was an ethanol extract of the stem bark against S. epidermidis and P. acnes with MIC value 4 mg/ml and 2 mg/ml, respectively. The extract also induced protein and ion leakage in test bacterial cells. SEM findings revealed that the extract induced potential cellular membrane damage in 4xMIC. Conclusion: The antibacterial activity was related to the damaged of bacterial cell wall.

}, keywords = {Cellular membrane damage., Morus nigra, Propionibacterium Acnes, Staphylococcus Epidermidis}, doi = {10.5530/pj.2018.1.28}, url = {http://fulltxt.org/article/414}, author = {Diah Lia Aulifa and Sani Nurlaela Fitriansyah and Seno Aulia Ardiansyah and Diki Prayugo Wibowo and Yolanda Armiliani Julata and Desty Santi Christy} } @article {226, title = {Anti-inflammatory activity of isolated compounds from the stem bark of Garcinia cowa Roxb}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {55-57}, type = {Original Article}, chapter = {55}, abstract = {

Objective: To find the anti inflammatory active compounds from methanol extract of Garcinia cowa. Methods: To evaluate the inhibitory activity of isolated compounds on nitric oxide (NO) production, culture media was assayed using Griess reaction. An equal volume of Griess reagent (1\% sulphanilamide and 0.1\% N-(L-naphthyl)-ethylene diamine dihydrochloride, dissolved in 2.5\% H3PO4) was mixed with culture supernatant and color development was measured at 550 nm using a micro plate reader. The amount of nitrite in the culture supernatant was calculated from a standard curve (0\–100 \μM) of sodium nitrite freshly prepared in deionized water. Percentage of the NO inhibition was calculated by using nitrate level of IFN-\γ/LPS-induced group as the control. Results: Isolated compounds, tetraprenyltoluquinone, rubraxanthone and \α-mangostin from stem bark of Garcinia cowa Roxb were evaluated for their anti-inflammatory activity. Only \α-mangostin exhibited strong anti-inflammatory activity with 83.42 \% inhibition of NO and without inducing severe cytotoxicity at 50M. Rubraxanthone showed weak inhibition of NO with 23.86 \% inhibition of NO while maintained 77.32 \% of cell viability. TPTQ also showed the strong inhibition of NO with 80.98 \% inhibition but unfortunately this compound also induced severe cytotoxicity with 39.62\% viability. Conclusion: \α-Mangostin exhibited strong anti-inflammatory activity without inducing severe cytotoxicity at 50 M. Rubraxanthone showed weak inhibition of NO while Tetraprenyltoluquinone also showed the strong inhibition of NO however this compound also induced severe cytotoxicity.

}, keywords = {Anti-inflammatory, Garcinia cowa, Nitric oxide, rubraxanthone, tetrapreniltoluquinone, α-mangostin}, doi = {10.5530/pj.2017.1.10}, author = {Fatma Sri Wahyuni and Daud Ahmad Israf Ali and Nordin Hj. Lajis and Dachriyanus} } @article {345, title = {Apoptosis Induction of Cervical Carcinoma HeLa Cells Line by Dichloromethane Fraction of the Rinds of Garcinia cowa Roxb}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {May 2017}, pages = {475-478}, type = {Original Article}, chapter = {475}, abstract = {

Objective: This study aims to determine the apoptosis induction of HeLa cervical carcinoma cells death by dichloromethane fraction of the rinds of Garcinia cowa Roxb. Methods: Apoptosis induction of HeLa cell line was observed using a double staining method. Results: The result of double staining observation showed that an apoptosis occurs which marked with yellowish green fluorescence and cell fragmentation. The average percentage of apoptotic cells was higher in the treated variables (70.38\%) compared to the control variables (12.26\% ). Statistical analysis by Independent Sample T-Test showed apoptosis Sig. (2-tailed) = 0.000 (\<0.025). Conclusion: The dichloromethane fraction of G. cowa rind induces apoptosis in HeLa cervical cancer cells.

}, keywords = {Apoptosis induction, Carcinoma cells, Double stanining, Garcinia cowa Roxb., HeLa cell line}, doi = {10.5530/pj.2017.4.76}, url = {/files/PJ-9-4/10.5530pj.2017.4.76}, author = {Fatma Sri Wahyuni and Siska Febria and Dessy Arisanty} } @article {254, title = {Chemical Profile and Antioxidant Properties of Mundulea sericea}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {213-220}, type = {Original Article}, chapter = {213}, abstract = {

Objectives: To evaluate the phytochemical composition and the antioxidant activity of aqueous-methanolic (20:80) leaves extract of Mundulea sericea Willd. Methods: The extract of leaves was tested for antioxidant activity using various in vitro models viz., 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide, ABTS, ferric reducing antioxidant power (FRAP), total antioxidant activity and reducing power. The phytochemical composition (GC-MS and HPLC) along with total phenolic and flavonoid content of the extract at different concentrations were also determined. Results: Total phenolic and flavonoid contents were found to be equivalents to 107.86 \± 0.53 g of gallic acid and 44.53 \± 0.156 g of rutin /mg of dried hydro-methanolic methanolic extract, respectively. Among various antioxidant assays performed, maximum inhibition was observed for ABTS (IC50 13.26 \± 0.396 g) followed by DPPH (IC50 79.83 \± 0.306 g) and NO (IC50 6.35 \± 0.23 g/mL) assay. The GC-MS analysis revealed over 38 compounds; the prevailing compounds were Sec- Butyl ethyl sulfoxide and Di-methyl sulfoxonium formyl methylide. The RP-HPLC analysis further confirmed the presence of rutin, cinnamic acid and salicylic acid. Conclusion: The results of the present study demonstrated that Mundulea sericea leaves possess high phenolic with flavonoid contents and also revealed potential antioxidant activity so these leaves could be used as a viable source of natural antioxidants for industrial and pharmaceutical preparations.

}, keywords = {Antioxidant, Flavonoids, GC-MS, Leaf extract, Mundulea sericea, Phenolics, RP-HPLC}, doi = {10.5530/pj.2017.2.36}, url = {http://phcogj.com/fulltext/303}, author = {Mahendra Shivshankar Khyade and Mohan Baban Waman} } @article {258, title = {Effect of Gamma Irradiation on Suruhan (Peperomia pellucida (L.) Kunth) Herb Powder}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {239-243}, type = {Original Article}, chapter = {239}, abstract = {

Introduction: Peperomia pellucida (L.) Kunth is known as a raw material for herbal medicine; Preservation of herbs powder by gamma irradiation is reported to be able to keep products free from contamination. Methods: This study aims to evaluate the effect of gamma irradiation (0; 2.5; 5; 7.5; and 10 kGy) on the ACE inhibitory activity (ACE Kit \– WST test kit method), antioxidant activity (DPPH radical scavenging method), total phenolic content (colorimetric method using Folin-Ciocalteu reagent), total flavonoid content (colorimetric method using AlCl3 and sodium acetate), and TLC profiling (silica gel F254 as the stationary phase and dichlormethane:methanol [92:8] as the mobile phase) of suruhan herb powder. Results: Results showed that the 2.5 kGy irradiation dose gave the smallest alteration in ACE inhibitory activity compared to others irradiated doses. Furthermore, the 5 and 7.5 kGy dose didn\’t cause significant change (p\>0.05) on antioxidant activity, total phenolic content, and total flavonoid content. Antioxidant activity was found to correlate with the total phenolic content but not with the total flavonoid content. Conclusion: Based on these finding, it is concluded that gamma irradiation can be used as a preservation method for P. pellucida herb powder.

}, keywords = {Angiotensin converting enzyme, antioxidant activity, Peperomia pellucida, total flavonoid, total phenolic}, doi = {10.5530/pj.2017.2.40}, url = {http://phcogj.com/fulltext/307}, author = {Mubarika Sekarsari Yusuf and Intan Wulandari and Lili Amelia and Katrin and Arikadia Noviani and Rissyelly and Abdul Mun{\textquoteright}im} } @article {371, title = {GC-MS analysis of Tasmannia lanceolata Extracts which Inhibit the Growth of the Pathogenic Bacterium Clostridium perfringens}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {July 2017}, pages = {626-637}, type = {Original Article}, chapter = {626}, abstract = {

Introduction: Clostridium perfringens is the etiological agent of clostridial myonecrosis and enteritis necroticans. Infections result in exotoxin production, tissue necrosis and unless promptly treated, often result in death. Methods: Tasmannia lanceolata extracts were investigated for C. perfringens growth inhibitory activity by disc diffusion analysis and MIC determination. Toxicity was evaluated by Artemia nauplii bioassay and the most potent extracts were phytochemically evaluated by GC-MS headspace analysis. Results: All T. lanceolata berry and leaf extracts displayed potent C. perfringens growth inhibition. The berry extracts were more potent growth inhibitors than the corresponding leaf extracts, although the leaf extracts were also potent growth inhibitors. The berry aqueous, methanolic and ethyl acetate extracts were particularly potent growth inhibitors, with MIC values of 654, 65 and 329 \μg/mL respectively. T. lanceolata leaf also displayed good efficacy, with an MIC of 839, 1255 and 625 \μg/mL for the aqueous, methanolic and ethyl acetate extracts respectively. All extracts were nontoxic in the Artemia franciscana bioassay, with LC50 values substantially \> 1000 \μg/mL. Non-biased GC-MS analysis of the aqueous, methanolic and ethyl acetate berry extracts revealed the presence of high relative levels of a diversity of terpenoids. Conclusions: The lack of toxicity of the T. lanceolata extracts and their potent growth inhibitory bioactivity against C. perfringens indicates their potential as medicinal agents in the treatment and prevention of clostridial myonecrosis and enteritis necroticans. GC-MS metabolomic profiling studies indicate that these extracts contained a diversity of terpenoids, with monoterpenoids being particularly abundant.

}, keywords = {Enteritis necroticans, Gas gangrene, Myonecrosis, Tasmannia Lanceolata, Winteraceae}, doi = {10.5530/pj.2017.5.100}, url = {/files/pj-9-5/10.5530pj.2017.5.100/index.html}, author = {Mitchell Henry Wright and Cameron Jay Lee and Megan Sarah Jean Arnold and Joseph Shalom and Alan White and Anthony Carlson Greene and Ian Edwin Cock} } @article {230, title = {GC-MS headspace analysis of Terminalia ferdinandiana fruit and leaf extracts which inhibit Bacillus anthracis growth}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {73-82}, type = {Original Article}, chapter = {73}, abstract = {

Background: Terminalia ferdinandiana (Kakadu plum) is an endemic Australian plant with an extremely high antioxidant capacity. The fruit has long been used by the first Australians as a nutritional food and as a medicine and recent studies have reported its potent growth inhibitory activity against a broad panel of bacteria. Despite this, T. ferdinandiana extracts are yet to be tested for the ability to inhibit the growth of Bacillus anthracis. Materials and Methods: Solvent extracts were prepared using both the fruit and leaf of Kakadu plum. The ability to inhibit the growth of B. anthracis was investigated using a disc diffusion assay. Their MIC values were determined to quantify and compare their efficacies. Toxicity was determined using the Artemia franciscana nauplii bioassay. The most potent extracts were investigated using non-targeted GC-MS head space analysis (with screening against a compound database) for the identification and characterisation of individual components in the crude plant extracts. Results: Solvent extractions of T. ferdinandiana fruit and leaf displayed good growth inhibitory activity in the disc diffusion assay against B. anthracis. Fruit ethyl acetate and methanolic leaf extracts were particularly potent growth inhibitors, with MIC values of 451 and 377\μg/mL respectively. The fruit methanolic and chloroform extracts, as well as the aqueous leaf extracts also were good inhibitors of B. anthracis growth, albeit with lower efficacy (MIC values of 1800 and 1414 \μg/mL respectively).The aqueous fruit extract and leaf chloroform extracts had only low inhibitory activity. All other extracts were completely devoid of growth inhibitory activity. Furthermore, all of the extracts with growth inhibitory activity were nontoxic in the Artemia fransiscana bioassay, with LC50 values \>1000 \μg/mL. Non-biased GC-MS phytochemical analysis of the most active extracts (fruit ethyl acetate and methanolic leaf) putatively identified and highlighted several compounds that may contribute to the ability of these extracts to inhibit the growth of B. anthracis. Conclusions: The low toxicity of the T. ferdinandiana fruit ethyl acetate and methanolic leaf extracts, as well as their potent growth inhibitory bioactivity against B. anthracis, indicates their potential as medicinal agents in the treatment and prevention of anthrax.

}, keywords = {Anthrax, Bacillus anthracis, Combretastatin, Kakadu plum, Metabolomics., stilbene, Tannin, Zoonotic}, doi = {10.5530/pj.2017.1.14}, author = {Mitchell Henry Wright and Joseph Sirdaarta and Alan White and Anthony Carlson Greene and Ian Edwin Cock} } @article {240, title = {HPLC Fingerprint Analysis and Content Determination of Extract with Anticancer Activities of Sedi Linearis Herba}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {128-134}, type = {Original Article}, chapter = {128}, abstract = {

Aims: The purpose of this paper was to develop a HPLC fingerprint analysis method, compare the chromatographic fingerprints characteristics of the ethyl acetate extract with anticancer activity from Sedi Linearis Herba (Sedum lineare Thumb.) collected in different seasons and environments, determine the contents of active ingredients hyperoside, isoquercetin and astragalin of each sample by HPLC, provide believable scientific foundations for quality control and evaluation of Sedi Linearis Herba. Methods: The analyte was analyzed using BDS Hypersil C18 as chromatographic column, acetonitrile -0.1\% acetic acid solution as the mobile phase (gradient elution). Results: The results showed that the major active ingredient contents of the samples collected in different seasons but at the same environment all had obvious differences. These ingredient contents of the samples collected in the end of April were much more than those in mid-August in general. Moreover, the major active ingredient contents of the samples picked in the same season but different environments were different too. For instance, the major ingredient contents of the sample in damp environment in August were more than those in sunny environment. Conclusion: All of these indicated that the HPLC fingerprint analysis and contents determination method established were very useful for quality analysis and control of Sedi Linearis Herba.

}, keywords = {Anticancer Activity Ingredients, Content Determination, HPLC Fingerprint, Sedum lineare Thunb.}, doi = {10.5530/pj.2017.2.22}, url = {http://phcogj.com/fulltext/289}, author = {Diangang Liu and Qing Mei and Weifang Long and Xiangluan Wan and Dingrong Wan and Luyao Wang} } @article {496, title = {HPTLC Analysis and Antiproliferative Effect of Various Extracts of Swertia alata on Growth of Leishmania donovani Promastigotes in vitro}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {November 2017}, pages = {s107-s116}, type = {Original Article}, chapter = {s107}, abstract = {

The present study was carried out to evaluate the quality control analysis and antiproliferative effects of petroleum ether, chloroform, ethanol and aqueous extracts of Swertia alata (family Gentianaceae) on Leishmania donovani. A basic, exact, quick and reproducible high performance thin layer chromatography (HPTLC) has been created for synchronous analysis of Oleanolic acid and Swertiamarin from S. alata.

Read more...}, keywords = {Antiproliferative, HPTLC, Oleanolic acid, Quality control, Swertiamarin}, doi = {10.5530/pj.2017.6s.166}, url = {http://fulltxt.org/article/391}, author = {Sakshi Bajaj and Sharad Wakode and Washim Khan} } @article {343, title = {Inhibition of Caco-2 and HeLa proliferation by Terminalia carpentariae C. T. White and Terminalia grandiflora Benth. extracts: Identification of triterpenoid components}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {May 2017}, pages = {441-451}, type = {Original Article}, chapter = {441}, abstract = {

Background: Terminalia spp. are characterised by their high antioxidant capacities and many have anticancer activity. This study examines the anti-proliferative activity of T. carpentariae leaf and T. grandiflora leaf, fruit and nut extracts against Caco-2 and HeLa carcinoma proliferation. Materials and Methods: Powdered T. carpentariae leaf and T. grandiflora leaf, fruit and nut were extracted and tested for anti-proliferative activity against Caco-2 and HeLa cancer cell lines using colorimetric cell proliferation assays. Toxicity was evaluated using an Artemia franciscana nauplii bioassay. The extract with the most potent anti-proliferative activity was examined using GCMS analysis and triterpenoid compounds were identified by comparison with a compound database. Results: T. carpentariae leaf and T. grandiflora leaf, fruit and nut extracts displayed potent anti-proliferative activity against Caco-2 and HeLa carcinoma cells. The methanolic T. grandiflora leaf extract was particularly effective at blocking the proliferation of the colorectal carcinoma Caco-2 (IC50 = 372 \μg/mL). The methanol T. carpentariae and T. grandiflora leaf extracts were similarly potent inhibitors of HeLa cervical cancer cell proliferation with IC50 values of 864 and 833 \μg/mL respectively. The methanolic T. grandiflora fruit and nut extracts, as well as all aqueous and ethyl acetate extracts, were moderate to good inhibitors of carcinoma proliferation. In contrast, chloroform and hexane extracts were generally devoid of anti-proliferative activity. The methanolic T. grandiflora extracts displayed low toxicity in the Artemia nauplii bioassay. All other extracts were non-toxic. GC-MS analysis of the methanolic T. grandiflora leaf extract identified 3 lanostane and 2 pentacyclic triterpenoids. Conclusion: The low toxicity and anti-proliferative activity observed with the T. carpentariae and T. grandiflora extracts against Caco-2 and HeLa indicate their potential for the prevention and treatment of some cancers.

}, keywords = {Anticancer activity, Australian plants, Caco-2, Chemotherapy, Combretaceae, HeLa, Native almond, Wild peach}, doi = {10.5530/pj.2017.4.74}, url = {/files/PJ-9-4/10.5530pj.2017.4.74}, author = {Reece Courtney and J. Sirdaarta and A. White and I. E. Cock} } @article {246, title = {Lipid Peroxidation Inhibitory Activity In vitro of Mezzetia parviflora Becc. Wood Bark Polar extract}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {171-175}, type = {Original Article}, chapter = {171}, abstract = {

Introduction: The wood bark of Mezzetia parviflora Becc, has long served as one of the most important traditional herbal medicine sources in Buton Regency, Southeast Sulawesi. M. parviflora extracts were rich in polyphenols. This study was aimed to explore the lipid peroxidation inhibitory activity of polar extract of M. parviflora. Methods: The polar extract is the result of ethanol extract partition solved in acetone. The extract will keep polar components which are insoluble in acetone. Assayed methods applied are \ß-carotene bleaching inhibition, thiobarbituric acid reactive substance (TBARS) measurement, and continuous monitoring of conjugated dienes formation in LDL. Results: M. parviflora extract inhibit \ß-carotene/ linoleic acid oxidation, showed by IC50 value of 15.83 g/ml in 30th minute; but the potency will be reduced to IC50 value of 111.19 g/ml and 225.07 g/ml after the 60th and 120th minute of incubation. M. parviflora extract inhibit MDA formation as for linoleic acid peroxidation product until the third day; at 20, 40, 60, 80 and 100 g/ml inhibit MDA formation as many as 29.16 \± 2.41\%, 4.24\% \± 43.27, 54.08 \± 2.87\%, 59.88 \± 1.90\%, and 69.75 \± 2.32\%, respectively. M. parviflora extract at 50 g/ml can inhibit LDL-oxidation induced by CuSO4, performed by LDL-oxidation lag-time elongation until 70 minutes, similar ability was performed by epigallocathecin gallate at 5 g/ml. Conclusions: M. parviflora extract expressed relatively strong protection against lipid and LDL oxidation which can serve as the scientific basis of its development as a remedy for various diseases caused by lipid peroxidation.

}, keywords = {Conjugated diene, Low-density lipoprotein, Malondilaldehyde, Mezzetia parviflora Becc, Polyphenol}, doi = {10.5530/pj.2017.2.28}, url = {http://phcogj.com/fulltext/295}, author = {Mufidah Murdifin and Ermina Pakki and Gemini Alam and Marianti A. Manggau and Lukman Muslimin and M. Rusdi and Elly Wahyudin} } @article {353, title = {Phyto-Pharmacognostical Studies and HPTLC Fingerprinting Profile of Uvaria narum (Dunal) Wall. Ex Wight}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {May 2017}, pages = {528-533}, type = {Original Article}, chapter = {528}, abstract = {

Objective: To study the pharmacognostic characters and phytochemical investigation of medicinally important crude drug, Uvaria narum (Dunal) Wall. Ex Wight (Annonaceae) Methods: Organoleptic characters, microscopic evaluation, leaf constants, quantitative physico-chemical evaluations (Ash value, extractive value, moisture content), preliminary phytochemical screening, quantitative estimation of total phenolic and flavonoid content in alcoholic and aqueous extract of Uvaria narum and HPTLC fingerprinting profile were studies on leaves of Uvaria narum by employing standard methods of analysis. Results: Leaf is dorsiventral, having distinct upper and lower surface. Stomata are of paracytic type and present only on lower epidermis. Upper epidermal cells are polygonal with cuticle and contain simple starch grains. A single layer of palisade cells is present below upper epidermis. Leaf do not bears any trichomes. Mid rib region shows collenchymatous cells on both surfaces. Xylem bundles are arranged in an arc. The space between collenchymas and vascular bundle is occupied by parenchymatous cells, some of which are filled with calcium oxalate crystals of prism as well as of rosette type (30-40 m in diameter). HPTLC fingerprint study of alcoholic and aqueous extracts confirmed the presence of quercitin as biomarker polyphenolic compound. Conclusion: These findings will be useful in establishing pharmacognostic and phytochemical standards for correct identification, as well as assessment of purity, quality of this plant, which definitely gaining the relevance in plant drug research and establishment of plant monograph.

}, keywords = {Histochemical evaluation, HPTLC fingerprinting, Morpho-Anatomical, Physicochemical, Uvaria Narum}, doi = {10.5530/pj.2017.4.85}, url = {/files/PJ-9-4/10.5530pj.2017.4.85}, author = {Kalpana Sabanna Patil and Raju Ratan Wadekar} } @article {440, title = {Quantitative Analysis of Irigenin in the Different Species of Iris Plant by RP- HPLC and its Efficacy Against Different Plant Pathogens}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {November 2017}, pages = {s23-s27}, type = {Original Article}, chapter = {s23}, abstract = {

Irigenin belongs to family of isoflavonoids, isolated from Iris Plant of Kashmir Himalaya. A rapid and specific reverse phase high pressure chromatography (RP HPLC) method for quantitative analysis of irigenin in the different species of Iris Plant was developed. The samples were analyzed on RP-C18 e column (chromolith, 5\μm, 4.6\×100 mm). The HPLC system was operated at ambient temperature (\±30c). The mobile phase consisted of methanol: water. The detecting wavelength at 260 nm and flow rate of 0.6 ml/min. The standard irigenin was diluted using the mobile phase at a known concentration of 1mg/ml; the sample was filtered through sample filter of 0.45 \μ pore size. The filtrate was introduced on to a reverse phase analytical column. The content of irigenin in the different species of Iris Plant was determined. The HPLC showed an excellent performance in separating the irigenin in different species of Iris Plant. Furthermore, the antipathogenic activity. The test compound at each respective concentration was found to be statistically superior against scab. Furthermore, the test compound @ 5000 ppm proved significantly most effective by providing (82.49\%) inhibition in the mycelia growth of apple scab. It was followed by fusarium (77.27\%) at 5000 ppm. Lowest reduction in mycelia growth (65.78\%) was recorded in marssonina and did not differ significantly from Alternaria (67.47\%) at 5000 ppm. Furthermore, lowest inhibition of mycelia growth was recorded at 1000 ppm. Similar trend was recorded for rest of the pathogens i.e. highest reduction at 5000 ppm, lowest at 1000 ppm and at 2000, 3000 and 4000 ppm it ranges between the first two but increases with increase in concentration. From this study irigenin is potent compound which can be used for controlling the growth of respective pathogens.

}, keywords = {Bio efficacy, Irigenin, Iris Plant, RP-HPLC}, doi = {10.5530/pj.2017.6s.153}, url = {http://fulltxt.org/article/377}, author = {Sajad Hassan Wani and Hilal Ahmad Bhat and Javid Iqbal Mir and Shahid Ali Akbar and Sajad Un Nabi and Desh Beer Singh and Nazeer Ahmad} } @article {326, title = {Sub-acute Toxicity Study of The Ethyl Acetate Fraction of Asam Kandis Rinds (Garcinia cowa Roxb.) on the Liver and Renal Function in Mice}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017 }, pages = {345-349}, type = {Original Article}, chapter = {345}, abstract = {

Objective: The present study investigated the sub acute toxicity of the ethyl acetate fraction of asam kandis (Garcinia cowa Roxb) Rinds in mice. Material and Methods: Sub acute toxicity study was carried out by giving orally at dose 500, 1000 dan 2000 mg / kgBW extract to five mice at 21 days. Animals were observed individually for any clinical signs of toxicity or mortality for 14 days. Measured parameters were SGPT levels, serum creatinine levels, weight ratio of liver and kidney. Extract was given orally at dose 500, 1000 and 2000 mg/kgBW for 21 days. Observations were done on day 8th, 15th and 22th using blood serum, liver and kidneys of mice. Data were analyzed by using two-way ANOVA followed by Duncan\’s Multiple Range Test. Results: The ethyl acetate fraction of G. cowa at doses 500, 1000 and 2000 mg/kgBW gave significant effect on increasing SGPT levels and decreasing levels of serum creatinine (p \<0.05). The length of treatment gave significant effect on decreasing levels of serum creatinine, weight ratio of liver and kidney (p \<0.05). Conclusion: The dosage of the ethyl acetate fraction of asam kandis rinds provides significant effect on the SGPT and serum creatinine levels of male white mice. The duration of administration of ethyl acetate fraction of asam kandis rinds provides significant effect on serum creatinine levels, the weight ratio of liver and kidney organ of male white mice.\ 

}, keywords = {Creatinine serum, Garcinia cowa rinds, SGPT, Sub-acute toxicity, Weight ratio of liver and kidney}, doi = {10.5530/pj.2017.3.58}, url = {/files/PJ-9-3/10.5530pj.2017.3.58}, author = {Fatma Sri Wahyuni and Dessy Arisanty and Nelsi Fitri Hayaty and Dian Ayu Juwita and Almahdy} } @article {149, title = {Bacillus anthracis growth Inhibitory Properties of Australian Terminalia spp.: Putative Identification of low Polarity Volatile Components by GC-MS Headspace Analysis}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {Jan/2016}, pages = {281-290}, type = {Original Article}, chapter = {281}, abstract = {

Introduction: Anthrax is a severe acute disease caused by Bacillus anthracis infections. If untreated, it often results in mortality. Many Terminalia spp. have documented therapeutic properties as general antiseptics, inhibiting the growth of a wide variety of bacterial species. This study examines the ability of selected Australian Terminalia spp. extracts to inhibit B. anthracis growth. Methods:\ Solvent extracts were prepared from Terminalia carpentariae and Terminalia grandiflora plant material and investigated by disc diffusion assay for the ability to inhibit the growth of an environmental strain of B. anthracis. Their MIC values were determined to quantify and compare their efficacies. Toxicity was determined using the Artemia franciscana nauplii bioassay. The most potent extracts were analysed by GC-MS headspace analysis. Results: T. carpentariae and T. grandiflora leaf, fruit and nut solvent extractions displayed good growth inhibitory activity against B. anthracis. Methanolic T. Carpentariae leaf and T. grandiflora nut extracts were particularly potent growth inhibitors, with MIC values of 74 and 155 \µg/mL respectively. The T. carpentariae leaf ethyl acetate extract was also a good inhibitor of B. anthracis growth (MIC 340 \µg/mL). All other extracts were substantially less potent growth inhibitors. Interestingly, the T. Carpentariae leaf extracts with growth inhibitory activity were nontoxic in the Artemia fransiscana bioassay, with LC50 values \>1000 \µg/mL. In contrast, the LC50 value 740 \µg/mL reported for the methanolic T. grandiflora nut extract indicates low-moderate toxicity. Non-biased GC-MS phytochemical analysis of the most active extracts (methanolic T. carpentariae leaf and T. grandiflora nut) putatively identified and highlighted several compounds that may contribute to the ability of these extracts to inhibit the growth of B. anthracis. Conclusions: The growth inhibitory activity of the methanolic T. Carpentariae leaf and T. grandiflora nutextracts against B. anthracis indicates their potential for the treatment and prevention of anthrax. Furthermore, thelack toxicity of the T. Carpentariae leaf and the low-moderate toxicity of the T. grandiflora nut extract, indicates that their use may extend to all forms of the disease (cutaneous, inhalation or gastrointestinal).

}, keywords = {Anthrax, Combretaceae, Metabolomic profiling., Native almond, Terminalia carpentariae, Terminalia grandiflora, Wild peach}, doi = {10.5530/pj.2016.3.18}, author = {Mitchell Henry Wright and Joseph Sirdaarta and Alan White and Anthony Carlson Greene and Ian Edwin Cock} } @article {162, title = {Cakile maritima Scop. extracts inhibit the growth of some bacterial triggers of autoimmune diseases: GC-MS analysis of an inhibitory extract}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {June/2016}, pages = {361-374}, type = {Original Article}, chapter = {361}, abstract = {

Introduction: High antioxidant capacities have been linked to the treatment of rheumatic diseases and also in the inhibition of microbial growth. Although Cakile maritima has a high antioxidant capacity, it is yet to be tested for the ability to inhibit the growth of the bacterial triggers of autoimmune inflammatory diseases. Methods: C. maritima solvent extracts were analysed for antioxidant capacity by the DPPH free radical scavenging assay. Growth inhibitory activities against bacterial species associated with initiating rheumatoid arthritis, ankylosing spondylitis and multiple sclerosis were determined by disc diffusion assay and quantified by MIC determination. Toxicity was determined by Artemia franciscana bioassay. Results: All C. maritima solvent extracts displayed good DPPH radical scavenging activity, although the ethyl acetate extract was particularly potent with an IC50 values of 3.4 \μg/mL. The other extracts also had significant radical scavenging activity, with IC50 between 4.7 and 13.6 \μg/mL. The bacterial growth inhibitory activity of the extracts correlated with their free radical scavenging activity. The ethyl acetate extract displayed the most potent growth inhibitory activity against most bacterial species. This extract was particularly potent against Proteus mirabilis, Proteus vulgaris and Pseudomonas aeruginosa (MIC values of 431, 559 and 777 \μg/mL, respectively). The hexane extract was also a potent inhibitor of the Proteus spp., (MIC of approximately 500-800 \μg/mL). The ethyl acetate extract also inhibited Klebsiella pneumoniae growth, albeit with higher MIC\’s (approximately 1500 \μg/mL). All other C. maritima extract-bacteria combinations generally resulted in mid-low potency inhibition. All of the extracts were determined to be nontoxicin with the Artemia franciscana bioassay, with LC50 values substantially \>1000 \μg/mL. A total of 97 unique mass signals were detected in the C. maritima ethyl acetate extract by nonbiased GC-MS headspace analysis. A number of terpenoids which may contribute to the therapeutic bioactivities of the extract were putatively identified. Conclusion: The lack of toxicity and the inhibitory activity against microbial triggers of rheumatoid arthritis, ankylosing spondylitis and multiple sclerosis by the C. maritima ethyl acetate extract indicates its potential in the treatment and prevention of these diseases.

}, keywords = {Acinitobacter baylyi, ankylosing spondylitis, Klebsiella pneumoniae, multiple sclerosis, Proteus mirabilis, Proteus vulgaris, Pseudomonas areuginosa., rheumatoid arthritis}, doi = {10.5530/pj.2016.4.9}, author = {Elsayed Omer and Abdelsamed Elshamy and Abdel Nasser El Gendy and Xin Cai and Joseph Sirdaarta and Alan White and Ian Edwin Cock} } @article {136, title = {GC-MS Analysis of Commiphora molmol Oleo-Resin Extracts which Inhibit the growth of Bacterial Triggers of Selected Autoimmune Diseases.}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {January 2016}, pages = {191-202}, type = {Original Article}, chapter = {191}, abstract = {

Introduction: Myrrh has been used traditionally for the inhibition of microbial growth and for the treatment of rheumatic diseases. Despite this, myrrh extracts are yet to be tested for the ability to inhibit the growth of the bacterial triggers of autoimmune inflammatory diseases. Methods: Solvent extracts prepared from commercially obtained myrrh resin were analysed for the ability to inhibit the growth of bacterial species associated with initiating rheumatoid arthritis (P. mirabilis), ankylosing spondylitis (K. pneumoniae) and multiple sclerosis (A. baylyi, P. aeruginosa) by disc diffusion assay, and quantified by MIC determination. Toxicity was determined by Artemia franciscana bioassay. The most potent inhibitory extract was investigated using non-targeted GC-MS head space analysis (with screening against a compound database) for the identification and characterization of individual components in the crude plant extracts. Results:\ Methanolic myrrh extract inhibited the growth of all bacterial species tested. The growth inhibition of this extract was particularly notable against P. mirabilis and K. pneumoniae, with MIC values substantially \< 1000 \μg/mL for both reference and clinical bacterial strains. Indeed, the MIC values of the methanolic extract against P. mirabilis reference and clinical strains were 572 and 463 \μg/mL respectively. The methanolic extract also inhibited the growth of A. baylyi (MIC approximately 3000 \μg/mL) and P. aeruginosa (MIC approximately 1800 \μg/mL). However, the MICs against these bacteria was indicative of only moderate inhibitory activity. The aqueous, ethyl acetate, chloroform and hexane extracts also inhibited the growth of all bacterial species, albeit with moderate (MIC values 1000-5000 \μg/mL) to low efficacy (MIC values \>5000 \μg/mL) against all bacterial species. All myrrh extracts were non-toxicin the Artemia franciscana bioassay, with LC50 values substantially above 1000 \μg/mL. Non-biased GC-MS headspace\ analysis of the methanolic extracti dentified a high diversity of monoterpenoids and sesquiterpenoid. Conclusion: The lack of toxicity and the inhibitory activity of the methanolic myrrh extract against microbial triggers of rheumatoid arthritis, ankylosing spondylitis and multiple sclerosis indicates its potential in the treatment and prevention of these diseases.

}, keywords = {ankylosing spondylitis, Commiphora molmol, Monoterpenoid, Multiple sclerosis., Myrrh, rheumatoid arthritis, Sesquiterpenoid, Terpenoid}, doi = {10.5530/pj.2016.3.4}, author = {Isaac Biggs and Joseph Sirdaarta and Alan White and Ian Edwin Cock} } @article {130, title = {Growth Inhibitory Activity of Kakadu Plum Extracts Against the Opportunistic Pathogenclostridium Perfringens: New Leads in the Prevention and Treatment of Clostridial Myonecrosis}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {December 2015}, pages = {144-153}, type = {Original Article}, chapter = {144}, abstract = {

Introduction: Clostridium perfringens is the etiological agent of clostridial myonecrosis and enteritis necroticans. Infections result in exotoxin production, tissue necrosis and unless promptly treated, may result in death. Terminalia ferdinandiana (Kakadu plum) fruit has documented therapeutic properties as a general antiseptic agent. Fruit extracts have been reported to inhibit the growth of an extensive panel of pathogenic bacteria. Leaf extracts have also been shown to block the growth of several bacterial species associated with autoimmune inflammatory diseases. Methods: T. ferdinandiana fruit and leaf solvent extracts were investigated for growth inhibitory activity by disc diffusion assay against a clinical strain of Clostridium perfringens. Their MIC values were determined to quantify and compare their efficacies. Toxicity was determined using the Artemia franciscana nauplii bioassay. Active extracts were analysed by non-targeted HPLC-QTOF mass spectroscopy (with screening against 3 compound databases) for the identification and characterisation of individual components in the crude plant extracts. Results: Methanolic and aqueous T. ferdinandiana fruit and leaf extracts, as well as the leaf ethyl acetate extract, displayed growth inhibitory activity in the disc diffusion assay against C. perfringens. The leaf extracts were generally more potent growth inhibitors than the corresponding fruit extracts, although the aqueous fruit extract had substantially greater efficacy than the aqueous leaf extract. The methanolic and ethyl acetate leaf extracts were particularly potent growth inhibitors, with MIC values of 206 and 117 \μg/ml respectively. The fruit methanolic extract also displayed good efficacy, with an MIC of 716 \μg/ml. In contrast, the chloroform and hexane extracts of both fruit and leaf were completely devoid of growth inhibitory activity. All T. ferdinandiana extracts were either nontoxic or of low toxicity in the Artemia fransiscana bioassay. Non-biased phytochemical analysis of the methanolic and ethyl acetate leaf extracts revealed the presence of high relative levels of a diversity of galloand ellagi- tannins. Conclusion: The low toxicity of the T. ferdinandiana extracts and the potent growth inhibitory bioactivity of the leaf methanolic and ethyl acetate extracts against C. perfringens indicates their potential as medicinal agents in the treatment and prevention of clostridial myonecrosis and enteritis necroticans. Metabolomic profiling studies indicate that these extracts contained a diversity of tannins.

}, keywords = {Antibacterial extracts, Antioxidant, Australian medicinal plants, Enteritis necroticans, Gas gangrene., Kakadu plum, Myonecrosis, Terminalia ferdinandiana}, doi = {10.5530/pj.2016.2.8}, author = {Mitchell Henry Wright and Joseph Sirdaarta and Ben Matthews and Anthony Carlson Greene and Ian Edwin Cock} } @article {82, title = {Cytotoxicity Study of Ethanol Extract of the Leaves of Asam Kandis (Garcinia cowa Roxb.) on T47D Breast Cancer Cell line}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Nov-Dec 2015}, pages = {369-371}, type = {Original Article}, chapter = {369}, abstract = {

Objective: To investigate the cytotoxic effect of ethanolic extract of the leaves of asam kandis (Garcinia cowa Roxb.) against T47D breast cancer cells. Methods: The cytotoxicity of ethanol extract was carried out by measuring the activity of mitochondrial dehydrogenase in living cells that have ability to convert dissolved MTT pale yellow to purple formazan product. The extract was added at various concentrations (0.1, 1, 10 and 100 \μg/mL). The level of cytotoxicity was determined by calculating the IC50 value that was based on the percentage of the cell death after 24 hours treatment with the extract. Cell morphological changes were observed by using inverted microscope. Results: The IC50 value showed that ethanol extract of leaves of asam kandis could resist T47D breast cancer cells with IC50 6.13 \± 3.51 \μg/mL. The statistic results proved that ethanol extract of the leaves of asam kandis could inhibit the growth of T47D breast cancer cells significantly at concentrations of 10 \μg/mL and 100 \μg/mL. Conclusion: The results suggest that ethanol extract of the leaves of asam kandis was potential source of herbal medicine for cancer-related ailments.

}, keywords = {Asam kandis, Beast cancer, Cytotoxicity, Garcinia cowa Roxb, MTT Assay, T47D.}, doi = {10.5530/pj.2015.6.9}, author = {Fatma Sri Wahyuni and Dini Hara Triastuti and Helmi Arifin} } @article {75, title = {GC-MS Analysis of Bioactive Phytochemicals Present in Ethanolic Extracts of Leaves of Annona muricata: A Further Evidence for Its Medicinal Diversity}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {300-304}, type = {Original Article}, chapter = {300}, abstract = {

Background: Folk medicine has taken an important place especially in developing countries where limited health services are available. However, the absence of scientific evaluation of medicinal plants may cause serious adverse effects. Objective: To analyze the phytochemical composition of the ethanolic extracts of leaves of Annona muricata using gas chromatography mass spectroscopy (GC-MS). Materials and methods: GC-MS Analysis was used. Results: The GC-MS Analysis revealed 25 constituents of which 12 of the compounds were identified. The major constituents were two unidentified compounds with percentage peak areas of 23.51\% and 16.8\%. Of the identified compounds, the outstanding in composition were 7-Tetradecenal, (Z) (peak area 9.39\%), n-Hexadecanoic acid (peak area 7.12\%), Oleyl Alcohol (peak area 6.15\%), Phytol (peak area 5.61\%), cis, cis, cis-7,10,13-Hexadecatrienal (peak area 4.26\%), 2-Pentadecanol (peak area 3.93\%), 9,12-Octadecadienoic acid, ethyl ester (peak area 3.21\%), 1,2-Benzenedicarboxylic acid, butyl octyl ester (peak area 2.67\%), and 1,E- 11,Z-13-Octadecatriene (peak area 2.15\%), while the rest had less than 2\% composition by peak area. Conclusion: The current study suggests that ethanolic extracts of leaves of Annona muricata are a potent therapeutic agent and paves the way for the development of several treatment regimens based on compounds from this extract.

}, keywords = {Annona muricata, Ethanolic extracts, GC-MS, Medicinal diversity, Phytochemicals.}, doi = {10.5530/pj.2015.5.9}, author = {Yahaya Gavamukulya and Faten Abou-Elella and Fred Wamunyokoli and Hany A El-Shemy} } @article {88, title = {Inhibition of Bacillus anthracis growth by Australian native plants used traditionally as antibacterial medicines}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {389-396}, type = {Original Article}, chapter = {389}, abstract = {

Introduction: Anthrax is a zoonotic disease caused by the bacterium Bacillus anthracis. It is often fatal if left untreated. Many Australian plants have documented therapeutic properties as general antiseptics, inhibiting the growth of a wide variety of bacterial species. This study examines the ability of selected Australian plant extracts to inhibit B. anthracis growth. Methods: Solvent extracts were prepared using plants with documented ethnobotanical usage to treat bacterial infections, or published antibacterial activity. The extracts were investigated by disc diffusion assay for the ability to inhibit the growth of an environmental strain of B. anthracis. Their MIC values were determined to quantify and compare their efficacies. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: Methanolic and aqueous extracts of Eucalyptus baileyana and Eucalyptus major displayed potent antibacterial activity in the disc diffusion assay against B. anthracis. The methanolic extracts were particularly potent with MIC values as low as 290 \μg/mL (E. major methanolic extract). Tasmannia insipidia and Tasmannia stipitata extracts also inhibited B. anthracis growth, albeit with low efficacy. The E. baileyana and E. major methanolic leaf extracts as well as the E. baileyana aqueous leaf extract induced significant mortality in the Artemia fransiscana bioassay, with LC50 values substantially \<1000 \μg/mL, indicating the toxicity of these extracts. Conclusion: The potent inhibitory bioactivity of the E. baileyana and E. major extracts against B. anthracis demonstrate their potential as medicinal agents in the treatment and prevention of anthrax. However, their toxicity indicates that their use may be limited to the treatment of the cutaneous form of the disease, or for sterilisation of infected sites.

}, keywords = {Anthrax, Antibacterial activity, Bacillus anthracis, Eucalyptus, Scaevola spinescens, Tasmannia stipitata, Traditional medicine., Zoonotic}, doi = {10.5530/pj.2015.6.13}, author = {Mitchell Henry Wright and Anthony Carlson Greene and Ian Edwin Cock} } @article {63, title = {Iron Reducing and Radical Scavenging Activities of 13 Medicinal Plants From C{\^o}te d{\textquoteright}Ivoire}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {266-270}, type = {Original Article}, chapter = {266}, abstract = {

Objective: Oxidative stress has been involved in the development of varied human diseases. The aim of this study was to evaluate the iron reducing power and the antiradical activity of 13 plants traditionally used as medicinal plants in C\ôte d\’Ivoire. Materials and Methods: FRAP (ferric reducing antioxidant power) and ABTS (2,2\’-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid)) assays were used to assess the antioxidant property of 80\% methanol extracts prepared from the 13 plants. Results: A high iron reducing activity was exhibited by extracts from leaves of Leea guineensis (42.76 \± 28.54 mg of TE/gdry extract) and Bersama abyssinica (39.77 \± 31.29 mg of TE/g dry extract). Smeathmannia pubescens (\% ABTS = 92.44 \± 12.93\%), L. guineensis (\%ABTS = 89.73 \± 15.10\%), Keetia venosa (\% ABTS = 88.78 \± 17.36 \%) and Sapium ellipticum (\%ABTS = 85.86 \± 25.10\%), showed promising antiradical activity with IC50 values of 4.50, 5.00, 5.40 and 5.70 \μg/mL respectively. These values are (p \< 0.05) close to those of Trolox (CI50 = 4.10 \μg/mL) and ascorbic acid (CI50 = 4.90 \μg/mL). Conclusion: Our findings confirm the traditional use of the studied plants in treatment of various ailments. The results obtained provide promising baseline information for using these medicinal plants for improving the health status of the population.

}, keywords = {ABTS, Antioxidants, C{\^o}te d{\textquoteright}Ivoire, FRAP, Iron., Medicinal plants}, doi = {10.5530/pj.2015.5.2}, author = {Ehouman Evans and Kon{\'e} Mamidou Witabouna and Tra Bi F{\'e}zan Honora and Bakayoko Adama} } @article {1454, title = {Iron Reducing and Radical Scavenging Activities of 13 Medicinal Plants From C{\^o}te d{\textquoteright}Ivoire}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {29th Apr, 2015}, pages = {204-211}, type = {Original Article}, chapter = {204}, abstract = {

Oxidative stress has been involved in the development of varied human diseases. The aim of this study was to evaluate the iron reducing power and the antiradical activity of 13 plants traditionally used as medicinal plants in C\ôte d\’Ivoire. FRAP (ferric reducing antioxidant power) and ABTS (2,2\’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays were used to assess the antioxidant property of 80 \% methanol extracts prepared from the 13 plants. A high iron reducing activity was exhibited by extracts from leaves of Leea guineensis (42.76 \± 28.54 mg of TE/gdry extract) and Bersama abyssinica (39.77 \± 31.29 mg of TE/g dry extract). Smeathmannia pubescens (\% ABTS = 92.44 \± 12.93 \%), L. guineensis (\%ABTS = 89.73 \± 15.10 \%), Keetia venosa (\% ABTS = 88.78 \± 17.36 \%) and Sapium ellipticum (\% ABTS = 85.86 \± 25.10 \%), showed promising antiradical activity with IC50 values of 4.50, 5.00, 5.40 and 5.70 \µg/mL respectively. These values are (p\< 0.05) close to those of Trolox (CI50 = 4.10 \µg/mL) and ascorbic acid (CI50 = 4.90 \µg/mL). Our findings confirm the traditional use of the studied plants in treatment of various ailments. The results obtained provide promising baseline information for using these medicinal plants for improving the health status of the population.

Key words: ABTS, Antioxidants, C\ôte d\’Ivoire, FRAP, Medicinal plants

}, author = {Ehouman Evans and Kon{\'e} Mamidou Witabouna and Bakayoko Adama and Tra Bi F{\'e}zan Honora} } @article {87, title = {Quantitative Determination and Variation Tendencies of Flavonoids in Five Selaginella Plant Drugs}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Nov-Dec 2015}, pages = {378-382}, type = {Original Article}, chapter = {378}, keywords = {Amentoflavone content, HPLC, Selaginella, Total flavonoids content, UV-Vis spectrophotometry, Variation tendencies}, doi = {10.5530/pj.2015.6.11}, author = {Weifang Long and Qi Ding and Yujie Chen and Jiqing Hu and Luyang Li and Fei Zhang and Dingrong Wan} } @article {1500, title = {Antiproliferative activity of crude extract and fractions obtained from Digera muricata on Hela cell lines of human cervix and A549 cell lines of Human Lung.}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {3rd Sept, 2014}, pages = {32-38}, type = {Original Article}, chapter = {32}, abstract = {

Digera muricata (Linn.) of family Amaranthaceae is an ethanobotanically important plant species traditionally used against various disorders. Cytotoxic potential of methanolic extract and its fractions were investigated against Hela and A549 cell lines. Crude extract of Digera muricata was prepared in methanol by Continuous Hot Soxhlation technique. Crude extract was fractionated into two organic and one aqueous fraction by the help of Column Chromatography. MTT assay was used to evaluate the reduction of viability of the cancer cell lines. Cell viability was inhibited by crude extract of Digera muricata in a dose dependent manner ranging from 25\μg/ml to 250\μg/ml. Apoptosis assays using nucleic acid stains namely PI exclusion assay and Hoestch/PI assay were performed by the help of fluorescence microscopy. Morphological analysis was done by calculation of Apoptotic ratio and Percentage apoptosis. Our results suggests that methanolic and aqueous fraction of the extract of Digera muricata can be good source of cytotoxic compounds.

Key words: 3-(4,5-dimethylthiazol-2yl)-2,4 diphenyltetrazolium bromide assay, A549 cell line, cytotoxic, Digera muricata, HeLa cell line.

}, keywords = {3-(4, 4 diphenyltetrazolium bromide assay, 5-dimethylthiazol-2yl)-2, A549 Cell Line, Cytotoxic, Digera muricata, HeLa cell line}, author = {Shazia Usmani and Arshad Hussain and A.H.A Farooqui and Mohd.Arshad and Sahabjada Siddiqui and Mohd.Ahmad and Shadma Wahab} } @article {1520, title = {Eurycoma longifolia Jack (Simarubaceae); Advances in Its Medicinal Potentials}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {26th May 2014}, pages = {1-9}, type = {Review Article}, abstract = {

Eurycoma longifolia Jack is a tall slender shrub-tree which is well-reputed among the natives of South East Asia for its potent aphrodisiac effect. Its root extracts have shown important biological activities such as antitumor, antimalarial, antibacterial, anti-diabetic, anti-hypertensive, Osteoprotective, and ergogenic which are mainly attributed to quassinoids. Commercially it is available in the form of drinks (along with other herbs), capsules, or loose powders. Based on available online databases it was realized that in spite of numerous reports on medicinal properties of E. longifolia, a review of recent developments regarding phytopharmacology, safety and toxicology, pharmacokinetics, and clinical applications was lacking. Therefore, this concise yet informative piece of work was prepared by pulling together trustworthy information from all the accessible published and unpublished scientific resources to serve as a reliable source of reference for future investigations.

Key words: Malaysian Ginseng, Eurycomanone, Tongkat Ali, Quassinoids, Ergogenic, Pasak Bumi.

}, keywords = {Ergogenic, Eurycomanone, Malaysian Ginseng, Pasak Bumi, Quassinoids, Tongkat Ali}, author = {Faramarz Majidi Wizneh and Mohd Zaini Asmawi} } @article {1491, title = {Evaluation of Antioxidant Activity of Five Medicinal Plants in Sri Lanka.}, journal = {Pharmacognosy journal}, volume = {6}, year = {2014}, month = {8th April 2014}, pages = {49-54}, type = {Original Article}, abstract = {

This study investigated on the antioxidant properties of five medicinal plants used in Sri Lanka, namely Solanum nigrum, Amaranthus spinosus, Elephantopuss caber, Amorphophallus campanulatus and Canna indica. The cold methanol plant extracts were screened for the antioxidant activity evaluating their 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical in scavenging ability. The total ascorbic acid content of the extracts was also evaluated. The IC50 values of the extracts revealed that Solanum nigrum had the best DPPH scavenging activity with a value of 37.63 \± 0.11\μg/ml and was better than that of the standard ascorbic acid. Amorphophallus campanulatus extract gave the highest ascorbic acid content of 143.03 \± 1.97 mg per 100 g of the extract. All five plants extract showed DPPH scavenging activity in the order of Solanum nigrum \> Elephantopus scaber\> Amorphophallus campanulatus\> Canna indica. The plant extracts did not show a direct correlation between the ascorbic acid content to the DPPH scavenging activity. These experimental results reveals that these extracts can be utilized in future as therapeutic agent against free radical induced oxidative stress.

Key words: Antioxidant activities, Ascorbic acid, DPPH, Medicinal Plants.

}, keywords = {Antioxidant Activities, Ascorbic acid, DPPH, Medicinal plants}, author = {K. N. Mahesh, and M. N. Wickramaratne, and D. B. M. Wickramaratne} }