@article {849, title = {ELISA Test on Cordia myxa L. Leaf Extract for alpha-Glucosidase Inhibitor}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {February 2019}, pages = {358-361}, type = {Original Article}, chapter = {358}, abstract = {

Aimed: Determine the potential of Cordia myxa L. leaf on inhibited α-glucosidase. Material: ELISA Kit, Ethanol 96\%, Colomn Chromatography, n-hexane, ethyl acetate, Glocobay{\textregistered}.\  Method: Sample from Cordia myxa L. leaf extracted by ethanol 96\% then evaporated to get the sticky extract. The sticky extract of Cordia myxa L. leaf fractionated by column chromatography with n-hexane, n-hexane: ethyl acetate (90:10; 80:20; 75:25; 70:30; 65:35; 60:40; 55:45; 50:50) Assay: The fractions assayed by ELISA (Enzyme-Linked Immunosorbent Assay) with acarbose (Glucobay {\textregistered}) as the comparator. Result: The results showed that the n-hexane fraction is the highest potency on inhibited α-glucosidase with the noncompetitive mechanism. The IC50 of n-hexane fraction is 0.53 ppm been while the acarbose is 6.85 ppm. Conclusion: The n-hexane fraction of Cordia myxa L. leaf has the highest potency to use for possible decrease blood glucose level.

}, keywords = {Acarbose, Cordia myxa L., ELISA, IC50, α-glucosidase}, doi = {10.5530/pj.2019.11.54}, author = {Ahmad Najib and Aktsar Roskiana Ahmad and Virsa Handayani} } @article {471, title = {Qualitative and Quantitative Test of Total Flavonoid Buni Fruit (Antidesma bunius (L.) Spreng) with UV-Vis Spectrophotometry Method}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {December 2017}, pages = {60-63}, type = {Original Article}, chapter = {60}, abstract = {

The aim of this research is to determine of total flavonoid content in the Buni fruit (Antidesma bunius L. Spreng) extract. The extract was produced by stratified maceration method with the different solvent, i.e n-Hexane, Ethyl acetate and ethanol. The analysis of chemical compound using chemical reagent and Thin Layer Chromatography (TLC) method. The method is used to determines total flavonoid contains Buni fruit (Antidesma bunius L.) extract was based on the amount of Rutin Equivalent (RE) were used. The result shows that the flavonoid content higher in the n-Hexane extract is 10.72 \%, then ethyl acetate extract is 7.9 \% and 3.56 \% ethanol extract was counted to or as a Rutin.

}, keywords = {Antidesma bunius L. Spreng, Flavonoid content, Spectrophotometry UV-VIS}, doi = {10.5530/pj.2018.1.12}, url = {http://fulltxt.org/article/367}, author = {La Hamidu and Aktsar Roskiana Ahmad and Ahmad Najib} } @article {367, title = {Antioxidant Activity and Isolation of Xanthine Oxidase Inhibitor from Ruellia tuberosa L. Leaves}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {July 2017}, pages = {607-610}, type = {Original Article}, chapter = {607}, abstract = {

Introduction: The leaves of Ruellia tuberosa L. have been known to demonstrate strong antioxidant and xanthine oxidase (XOD) inhibitory activities. The aim of this study was to isolate antioxidant and XOD inhibitor from the leaves of the plants. Methods: Isolation of antioxidant and XOD inhibitor were conducted using chromatography techniques. The structure of the isolated compound was elucidated by spectroscopic methods. Results: In this study, a flavonoid was isolated and characterized as methoxylated flavonoid based on the spectral data including UV, IR, GC-MS, and NMR. The compound demonstrated DPPH free radical scavenging activity with IC50 of 28.79 \μg/ml, and XOD inhibitory with IC50 of 0.67 \μg/mL. Conclusion: The isolated compound was determined as 5-hydroxy-3,7-dimethoxy-2-(4-((3S,4S,5S,6R)-4,5, 6-trihydroxy-2(hydroxymethoxy)-tetrahydro-2H-pyrane-3-iloxy) phenyl)-4H-chromen-4-on or camarosids. The isolated compound demonstrated strong DPPH free radical scavenging and XOD inhibitory activity.\ 

}, keywords = {Antioxidant, DPPH, Flavonoid., Ruellia Tuberosa, Xanthine Oxidase}, doi = {10.5530/pj.2017.5.96}, url = {/files/pj-9-5/10.5530pj.2017.5.96/index.html}, author = {Aktsar Roskiana Ahmad and Berna Elya and Abdul Mun{\textquoteright}im} }