@article {1692, title = {Compound Analysis and Genetic Study of Selected Plectranthus scutellarioides Varieties from Indonesia}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1516-1526}, type = {Research Article}, chapter = {1516}, abstract = {

Background: Plectranthus scutellarioides is one of medicinal plants in Indonesia, which has several hundred varieties but only one is known by local people as medicine. Objective: Six varieties of Plectranthus scutellarioides were analyzed for their total flavonoid content, chemical compound, and moleculer genetic. Methods: TFCs were analyzed using AlCl3 colorimetric method, chemical compounds were identified using TLC-scanning densitometer, GC-MS, and FTIR, moleculer genetic were observed using DNA barcoding rbcL gene. Results: The TFCs of trailing psycholeus, and flamingo varieties were higher than the other varieties. TLC-scanner densitometer showed that color blaze dark star, trailing psycholeus, and trailing queen had similar profiles, as did beale street, trailing rose, and flamingo. The GCMS results showed notable difference in trailing psycholeus and trailing queen which have 2-oleoylglycerol and 9(E),11(E)-conjugated linoleic acid in larger amounts than others, respectively. Multivariate analysis of the FTIR spectra showed the closeness of all varieties, except for beale street which had the lowest similarity with the others. Despite that, genetic studies using the rbcL gene and comparing the results with the P. scutellarioides gene in the database (MW538954.1) showed beale street was the most similar (99.52\%). The phylogenetic analysis showed that beale street and trailing psycholeus have the highest similarity among others. Conclusions: There is a slight difference in chemical composition between varieties as well as the genetic. Therefore, quality control or standardisation is needed in the use of this plant as a traditional medicine.

}, keywords = {Coleus scutellarioides, Densitometer, Flavonoid, FTIR, GC-MS, RbcL.}, doi = {10.5530/pj.2021.13.193}, author = {Ayun Dwi Astuti and Awaluddin Iwan Perdana and Rosdiana Natzir and Muhammad Nasrum Massi and Subehan and Gemini Alam} } @article {462, title = {Effects of Miana (Coleus scutellariodes (L) Benth) to Expression of mRNA IL-37 in Balb/c Mice Infected Candida albicans}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {December 2017}, pages = {16-19}, type = {Original Article}, chapter = {16}, abstract = {

Background: Miana (Coleus scutellariodes (L) Benth) are a herbal medicine with potential anti-inflammatory properties in patients infected with vulvovaginal candidiasis. The objective of this work was to the analyze IL-37 expression following administration of miana leaf extract (MLE) in an animal model of vulvovaginal candidiasis. Material and Methods: Adult Balb/c mice, aged 8\–12 weeks and weighing 30\–40 g, were divided into five groups. Four groups were administered Candida albicans via intravaginal inoculation with a diluted dose of 10-2/ ml and were treated with either MLE, a placebo, or ketoconazole; one group constituted the healthy control and was only treated with MLE. Real-time PCR was used to measure the expression of IL-37. Results: These findings indicated that a component within MLE may mediate its anti-inflammatory characteristics, as indicated by the increase in mRNA IL-37 expression in mice inoculated with C. albicans. The highest increase in fungal load to 101.6 CFU was observed in the placebo group at day 14. Whereas for the mice treated with MLE at 750 mg/kg b.w, the fungal load only increased to 30.0 CFU, similar to that of mice treated with ketoconazole (29.6 CFU). In the mice treated with MLE at 500 mg/kg b.w, the fungal load increased to 68.2 CFU. Conclusion:\ Fungiostatic effect of MLE 750 mg/kg BB is not less than ketoconazole and MLE may act as anti-inflammatory throught its role as an antioxidant so it could potentially be used as an alternative treatment in humans especially patients with vulvovaginal candidiasis.

}, keywords = {CFU, Interleukin-37 mRNA, Miana, Realtime PCR, Vulvovaginal Candidiasis}, doi = {10.5530/pj.2018.1.3}, url = {http://fulltxt.org/article/358}, author = {Marni Karo and Mochammad Hatta and WaOde Salma and Ilhamjaya Patellongi and Rosdiana Natzir} }