@article {507, title = {Anthelmintic Activity of Fruit Extract and Fractions of Piper longum L. In vitro}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {January 2018}, pages = {333-340}, type = {Original Article}, chapter = {333}, abstract = {

The present study was aimed to assess the in vitro ovicidal, larvicidal and adulticidal activity of methanolic extract and its fractions from fruits of Piper longum against strongyle ova, larvae and adult amphistomes respectively. The fruits of P. longum was identified and the accession number 006 was obtained. The phytochemical analysis revealed the presence of tannins, flavonoids, glycosides, phenolics, diterpenes and triterpenes in extract and fractions of P. longum. The extract and fractions were diluted serially in 6.25 per cent tween 80 to obtain concentrations of 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 3.91 and 1.95 mg/mL. Ivermectin and thiabendazole at 10 \μg/mL acted as positive controls and 6.25 per cent tween 80 as negative control. The methanolic extract was highly active against ova with IC50 of 0.026 mg/mL. The n-hexane fraction was potent in inducing larval mortality with IC50 of 1.383 mg/mL while chloroform fraction inhibited larval migration with IC50 of 1.796 mg/mL. Amphistomes were highly sensitive for methanolic extract of P. longum which possessed IC50 of 5.493 mg/mL Based on IC50 values, the methanolic extract was found to be most potent while chloroform fraction was effective against ova, larvae and also adults. GCMS analysis of potent methanolic extract revealed the presence of piperidinone, hydrocinnamic acid, ethylhexahydro azepine, methyleugenol, hexadecanoic acid and caryophyllene oxide which may have contributed for the anthelmintic activity. The acute oral toxicity study revealed mild vascular changes in liver. From the present study, it can be concluded that chloroform fraction of P. longum possessed maximum broad spectrum anthelmintic activity comparable to controls.

}, keywords = {Adulticidal, GCMS, IC50, Larvicidal, Ovicidal, Piper longum.}, doi = {10.5530/pj.2018.2.57}, url = {http://fulltxt.org/article/487}, author = {Krishnaprasad Ganapati Koorse and Sujith Samraj and Preethy John and Priya Manakkulaparambil Narayanan and Devi SS and Usha PTA and Surya Sunilkumar and Gleeja VL} } @article {768, title = {Characterization of the Anthelmintic Activity of Murraya koenigii (Linn.)}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {November 2018}, pages = {s100-s103}, type = {Original Article}, chapter = {s100}, abstract = {

Objective: To identify the most potent sub fractions(s) of the different extracts of the leaves of Murraya koenigii for the anthelmintic property. Methods: The dried leaves were subjected to soxhlet extraction using methanol, fractionated using n-hexane, chloroform, n-butanol and water. Preliminary phytochemical analysis was done using standard techniques. The potent fractions were subjected to TLC and the appropriate solvent was selected for flash chromatographic separation of the extract. The sub fractions were tested for their anthelmintic activity in vitro using egg hatch assay and larval motility assay on Haemonchus contortus eggs and the most potent fraction was found out. Results: Phytochemical analysis revealed the presence of phenolic, tannins and saponins in all extracts and the effect of the extracts could be due to these components. On TLC, toluene: ethyl acetate in 9:1 ratio was found to be the best mobile phase for hexane and chloroform fractions whereas cyclohexane: ethyl acetate at 6:4 was found suitable for butanol fraction. Of the sub fractions (SF), SF 3 and 11 of chloroform fraction showed better ovicidal activity whereas SF 2,6,7,32 and 37 showed best larvicidal activity. The larvae that were used for testing the larvicidal activity, were found to be sluggishly motile after half an hour incubation with the extract and were progressively dead on a dose dependent manner. Conclusion: The chloroform extract of Murraya koenigii and its sub fractions 2, 3,6, 7, 11, 32 and 37 possessed good anthelmintic activity and the isolation of active molecules is necessary for development of a novel anthelmintic.

}, keywords = {Anthelmintic, Egg hatch assay, Haemonchus contortus, Larval motility assay, Murraya Koenigii}, doi = {10.5530/pj.2018.6s.19}, author = {Sujith S and Priya MN and Deepa CK and Usha PTA} } @article {651, title = {Characterizationof the Anthelmintic Activity of Murraya koenigii (Linn.)}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {July/2018}, pages = {73-76}, type = {Original Article}, chapter = {73}, abstract = {

Objective:To identify the most potent sub fractions(s) of the different extracts of the leaves of Murraya koeniggi for theanthelmintic property.Methods: The dried leaves were subjected to soxhlet extraction using methanol, fractionated using n-hexane, chloroform, n-butanol and water. Preliminary phytochemical analysis was done using standard techniques. The potent fractions were subjected to TLC and the appropriate solvent was selected for flash chromatographic separation of the extract. The sub fractions were tested for their anthelmintic activity invitro using egg hatch assay and larval motility assay on Haemonchus contortus eggs and the most potent fraction was found out. Results:Phytochemical analysis revealed the presence of phenolic, tannins and saponins in all extracts and the effect of the extracts could be due to these components. On TLC, toluene: ethyl acetate in 9:1 ratio was found to be the best mobile phase for hexane and chloroform fractions whereascyclohexane: ethyl acetate at 6:4 was found suitable for butanol fraction. Of the sub fractions (SF), SF 3 and 11 of chloroform fraction showed better ovicidal activity whereas SF 2,6,7,32 and 37 showed best larvicidal activity. The larvae that were used for testing the larvicidal activity, were found to be sluggishly motile after half an hour incubation with the extract and were progressively dead on a dose dependent manner.Conclusions:The chloroform extract of Murraya koenigii and its sub fractions 2, 3,6, 7, 11, 32 and 37 possessed good anthelmintic activity and the isolation of active molecules is necessary for development of a novel anthelmintic.

Key words: Anthelmintic, Murraya koenigii, Haemonchus contortus, Egg hatch assay, Larval motility assay.

}, doi = {10.5530/pj.2017.3.14}, author = {Sujith S and Priya MN and Deepa CK and Usha PTA} }