@article {904, title = {In vitro Antidiabetic Activity of Methanolic Leaf Extract of Indianthus virgatus (Roxb.) Suksathan and Borchs by Glucose Uptake Method}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {July 2019}, pages = {674-677}, type = {Original Article}, chapter = {674}, abstract = {

Medicinal plants play a key role to cure many diseases from time immemorial. The usage of medicinal plants in traditional medicinal system is the vital process of India. Diabetes Mellitus is a systemic metabolic disease characterized by hyperglycemia, abnormal elevated levels of lipid and fat in blood and hypoinsulinaemia. The current epidemic of diabetes indicates the need of proper and effective medications that are limited in their potency to have many side effects. Thus, introduction of alternative and complementary medicine is now in picture. Objective: The main objective of this work is to evaluate the in vitro anti diabetic activity of methanolic extracts of Indianthus virgatus (Roxb.) Suksathan and Borchs in skeletal muscle cell line. Methods: The in vitro cytotoxicity was performed for leaf extract (Methanol) on L-6 (Rat skeletal muscle) cell line to find toxic concentration of the leaf extract by MTT assay. Glucose uptake activity of test substance was determined in differentiated L-6 cells. Results: In Glucose uptake assay, Methanol Extract exhibited moderate toxicity to skeletal muscle cell line and glucose uptake assay it shows dose dependent glucose uptake. Glucose uptake rate increased with the increasing concentration of the leaf extract. Conclusion: The results of the current study clearly demonstrated the antidiabetic potency of methanolic leaf extract obtained from Indianthus virgatus (Roxb.) Suksathan and Borchs. under in vitro model.

}, keywords = {Antidiabetic activity, In vitro cytotoxicity, Indianthus virgatus (Roxb.) Suksathan and Borchs, L-6 cell line, Methanolic leaf extract, MTT Assay, Skeletal muscle Cell Lines, Therapeutic agents}, doi = {10.5530/pj.2019.11.106}, author = {Sangeetha DN and S. Rajamani} } @article {562, title = {Antimicrobial Potential of Crude Extracts of Thespesia populnea L. Flower on Multiple Drug Resistant Opportunistic Pathogens in HIV/AIDS}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {590-597}, type = {Original Article}, chapter = {590}, abstract = {

Background: Thespesia populnea L. commonly known as tulip tree or \‘portia\’ tree has been traditionally used for different illnesses. Thespesia populnea L. is considered to have high tannin content and have high therapeutic value. Purpose: The antifungal and antibacterial potential of T. populnea L. should be checked on multiple drug resistant opportunistic pathogens Pseudomonas aeruginosa and Candida albicans in HIV/AIDS patients. Methods: In order to check the effect of antibiotics antibiogram was studied with antibiotic sensitivity discs. To study the major factor affecting resistance test on formation of biofilm was done by tube and plate methods. The dried flower powder was subjected to sauxlaut hot extraction and the crude extract was analysed by Gas Chromatography and Mass Spectrophotoscopy. Antimicrobial potential of the ethyl acetate extract of the flower was checked by well agar diffusion, UV Spectrometry for growth rate and docking of selected compounds on the microbes of our study by the application of a software. Results: The tested extracts and the selected compounds have showed significant results in the antimicrobial activities against the opportunistic pathogens in human. Higher the concentration of the extracts better is the inhibition of microbes. Conclusion: The study provides a scientific rationale for the traditional use in the management of opportunistic pathogens which are multidrug resistant.

}, keywords = {Antibiogram, Antimicrobial, Biofilm, Docking, Inhibition, Multidrug Resistant, Opportunistic Pathogens}, doi = {10.5530/pj.2018.3.97}, url = {http://fulltxt.org/article/530}, author = {Jancy Varghese and S. Rajamani and Betty Daniel} }