@article {1967, title = {Alkaloids from Pandanus amaryllifolius Roxb Leaf as Promising Candidates for Antidyslipidemic Agents: An in silico study}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {March 2023}, pages = {106-111}, type = {Original Article }, chapter = {106}, abstract = {

Introduction: The plant Pandanus amaryllifolius Roxb (pandan), has been shown to have antidyslipidemic potency. This study explored the potential of several alkaloids from pandan leaf as antidyslipidemia as well as their safety profile in silico. Methods: Analyses were carried out by studying the binding affinity of the alkaloids to 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, peroxisome proliferator activator receptor (PPAR) alpha and Niemann Pick C1 Like 1 (NPC1L1). The structures of the alkaloids were downloaded from the Pubchem database and optimized using the ChemDraw Professional 16.0 to obtain 3D structures in protein data bank (PDB) format. The in silico testing was based on the interactions of the alkaloids with the HMG-CoA reductase (PDB ID 1HW9), PPAR alpha (PDB ID 6LX4) and NPC1L1 (PDB ID 7DFZ) proteins, downloaded from the Research Collaboratory for Structural Bioinformatics (RSCB) PDB website (http://www.rcsb.org/pdb). The preparation of protein structures was performed using the Discovery studio 2021 client and Gromacs applications, while optimization of the 3D structure of the alkaloids was carried out with the ChemDraw professional 16.0. Finally, validation was completed using AutoDock application. The safety profile was assessed by pkCSM online tool. Results: The respective root mean square deviation (RMSD) values of the 1HW9, 6LX4 and 7DFZ proteins were 1.677, 0.918 and 1.706, respectively. The alkaloids pandanusine B, pandamarilactonine A, pandamarilactonine B had respective values of binding energy for HMG-CoA of -5.52, -5.51 and -5.46 kcal/mol. The binding energy of pandamarilactonine B, pandamarilactonine A and pandanamine for PPAR alpha were -9.14, -9.10 and -8.48 kcal/mol, respectively, with the corresponding energy for t NPC1L1 of -9.63, -9.71 and -8.54 kcal/mol. The toxicity tests indicated that the alkaloids were safe, pandamarilactonines had the highest LD50 (2.736 mol/ kg). Conclusion: The studied pandan alkaloids have potential antidyslipidemic activity by interacting with HMG-CoA reductase, PPAR alpha, and NPC1L1, with good safety profile.

}, keywords = {Alkaloids, Dyslipidemia, In Silico., Pandan, Pandanus amaryllifolius}, doi = {10.5530/pj.2023.15.14}, author = {Martohap Parotua Lumbanraja and Kusnandar Anggadiredja and Hubbi Nashrullah Muhammad and Neng Fisheri Kurniati} } @article {1015, title = {Comparison of Extraction Methods, Antioxidant Activities, Total Phenol in Seeds and Seed Shells of Kabau (Archidendron bubalinum (Jack) I.C. Nielsen) From Lampung and South Sumatra}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1278-1284}, type = {Original Article}, chapter = {1278}, abstract = {

Background: Kabau is one of the famous plants in Lampung and South Sumatra. Kabau is usually used as an ingredient to increase the aroma of cooking. Kabau is a plant belongs to the same genus with Archidendron pauciflorium (Benth.) IC. Nielsen and in the same family with Leucaena leucocephala L. Objective: The purpose of this research was to compare the total phenolic content and the antioxidant activity between seed and fruit carp (shell) of kabau grown in Lampung and South Sumatra. Method: Kabau seeds and the shells were obtained from Lampung and South Sumatra. Extractions were conducted by maceration and a continuous extraction using Soxhlet apparatus with ethanol as the solvent. Phenolic content and the antioxidant activity of the extract were then measured. Results: The antioxidant activity and high phenol levels were found in kabau shell extract obtained by maceration method with IC-50 value of seed shell from Lampung 17.61 μg/mL and 44, 7 μg / mL for those from South Sumatra. Phenol content of seed shell from Lampung 11.74 g GAE / 100 g and South Sumatra 5.88 g GAE / 100 g. Conclusion: Based on the research that has been done, it can be concluded that high antioxidant activity and high levels of total phenol were found in shell extract obtained by maceration of shell from Lampung and South Sumatra.

}, keywords = {Antioxidants, Archidendron bubalinum (Jack) Nielsen, Phenols, Seed shell, Seeds}, doi = {10.5530/pj.2019.11.198}, author = {Hesti Riasari and Sani Nurlaela Fitriansyah and Rika Hartati and Kusnandar Anggadiredja and Sukrasno} } @article {1049, title = {Ethanol Extract of Gardenia augusta (L.) Merr. Flowers Produces Sleep Improvement in Rat Model}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {November 2019}, pages = {1449-1454}, type = {Original Article}, chapter = {1449}, abstract = {

Introduction: Sleep disorder may have detrimental consequences on health and one of the treatments is the use of hypnotics. Unfortunately, hypnotics treatment may also be accompanied by side effects and lead to dependence. The present study aimed to investigate the sleep improving effects of ethanol extract of Gardenia augusta (L.) Merr. flowers in rat model. Methods: Rats were assigned into group receiving the extract (at 0.9 or 1.8 g/kg), the reference drug crocetin (at 0.9 mg/kg) or vehicle, once a day orally for 15 days. Results: Tested on day 1 and 14 of treatment, the rats receiving 1.8 g/kg of the extract showed improvements in sleep latency, sleep duration, sleep efficiency, wake episodes, and sleep cycle, which were significantly different from crocetin. Furthermore, tested on day 2 and 15 of treatment, the rats treated with 1.8 g/kg extract demonstrated superior sedative rating scale compared to crocetin. Conclusions: Results of the present study indicates the potential of Gardenia augusta (L.) Merr. flower extract to be used as an adjunct treatment for sleep disorder.

}, keywords = {Flowers, Gardenia augusta (L.) Merr., Improvement, Rats, Sleep}, doi = {10.5530/pj.2019.11.224}, author = {Kusnandar Anggadiredja and I Ketut Adnyana and Dewi Safitri and Siti Farah Rahmawati and Tomi Hendrayana} }