@article {1190, title = {Comparative Study on Pharmacognostical, Phytochemical Investigations and Quantification of Vasicine Content in the Extracts of Adhatoda vasica Nees and Adhatoda beddomei CB Clarke}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {884-896}, type = {Research Article}, chapter = {884}, abstract = {

Background: Adhatoda, a perennial shrub of family Acanthaceae are well-known medicinal plant for the treatment and management of respiratory disorders such as asthma and bronchitis. Adhatoda vasica and Adhatoda beddomei are the species of Adhatoda, has been widely used in Indian system of medicine. Although, phytochemical and pharmacological investigations were reported on A. vasica, there has been comparative investigations on different Adhatoda species are lacking. Objective: The study was undertaken to compare the pharmacognostical and phytochemical parameters of two species of Adhatoda for rapid identification and authentication of the plants. Materials and Methods: Pharmacognostical features were studied by macroscopic, microscopic studies and physicochemical analysis such as determination of foreign matter, ash value, extractive value and loss on drying. Phytochemical investigations were analysed using phytochemical screening, bioactive content determination, HPTLC fingerprint analysis and estimation of vasicine content by HPLC analysis. Results: Microscopic study differentiated the pharmacognostical features between two species by demonstrating the anatomical characteristics. Powder microscopy of A. vasica revealed the presence of diacytic stomata, glandular and non-glandular trichomes whereas rod shaped crystals were seen only in A. beddomei. Qualitative and quantitative phytochemical investigations revealed the presence and estimation of various phytoconstituents in both the species. HPTLC fingerprint profiling evaluated the number of constituents present in the extracts and HPLC analysis revealed high content of vasicine in A. vasica extracts when compared to A. beddomei. Conclusion: The present study provides the useful information to differentiate the plant species and can serve as a diagnostic tool for the standardization and identification of adulterant in the crude drug market.

}, keywords = {Adhatoda beddomei, Adhatoda vasica, HPTLC fingerprint, Pharmacognosy, Vasicine}, doi = {10.5530/pj.2020.12.126}, author = {Nandhini S and Ilango K} } @article {1186, title = {Simultaneous Quantification of Lupeol, Stigmasterol and β- Sitosterol in Extracts of Adhatoda vasica Nees Leaves and its Marketed Formulations by a Validated RP-HPLC Method}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {850-856}, type = {Research Article}, chapter = {850}, abstract = {

Background: Adhatoda vasica Nees (Acanthaceae) is a well-known medicinal plant used for the treatment of respiratory disorders such as asthma and bronchitis. Objective: To develop a simple and precise RP-HPLC method for the simultaneous assessment of lupeol, stigmasterol and β-sitosterol of various extracts of Adhatoda vasica Nees. Materials and Methods: The compounds were separated on RP-Phenomenex C18 (250mm{\texttimes}4.6mm; 5μ) column with a mobile phase comprising of 0.1\%v/v formic acid in water and methanol (28:82\%v/v) splashed at a flow of 0.8mL/min with PDA detector at 208nm. Results: The retention time of lupeol, stigmasterol and β-sitosterol was found to be 16.89, 18.26 and 20.72 minutes respectively. The amount of lupeol was abundant in hexane extract (0.952\%w/w) and formulation III (23.72ng/g) whereas, stigmasterol (0.285\%w/w) and β-sitosterol (8.649\%w/w) was highly abundant in chloroform extract and formulation I stigmasterol (2.57ng/g) and β-sitosterol (0.98ng/g). The optimized method was validated for different parameters and all the validated constraints were within the limits as per ICH guidelines. The proposed method was linear over the concentration range of 12.5-200μg/mL with correlation coefficients greater than 0.997. The LOD and LOQ values of lupeol, stigmasterol and β-sitosterol were found to be 0.66, 5.64 and 12.8μg/mL and 2.01, 17.10 and 36.62μg/mL respectively. Conclusion: To conclude, the developed method for the simultaneous estimation of lupeol, stigmasterol and β-sitosterol was simple, precise, accurate and thus reliable for the quality control investigations of crude drugs and its herbal formulations.

}, keywords = {Adhatoda vasica Nees, HPLC, Lupeol, Simultaneous quantification, Stigmasterol, β-sitosterol}, doi = {10.5530/pj.2020.12.122}, author = {Nandhini S and Ilango K} }