@article {1795, title = {Toll Like Receptor 4 Expression Profile in Mice Infected Mycobacterium Tuberculosis Given with Miana Leaves Extract (Coleus scutellarioides (L.) Benth) (Tuberculosis Preventive and Curative Mechanisms)}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {June 2022}, pages = {497-505}, type = {Original Article}, chapter = {497}, abstract = {

Introduction: Tuberculosis is an infectious disease of the respiratory tract caused by Mycobacterium tuberculosis. During Mycobacterium tuberculosis infection, pathogens modulate TLR-4 receptor flow signaling, suggesting possible involvement of TLR-4 in the regulation of the host immune response. This study aims to determine the effect of miana leaf extract on the expression of toll like receptor 4 (TLR-4) in tuberculosis mice at the preventive and curative stages. Methods: Mice were divided into 3 groups with 7x replication. Providing 14 days of preventive treatment, 14 days of incubation and 14 days of curative treatment. Group 1 and group 3 were given Miana Leaves Extract (EDM) and placebo at all three stages. Group 2 was given EDM at the preventive and incubation stages, then they were given anti-tuberculosis drugs (OAT). Results: The results proved that EDM given as a preventive did not increase the expression of TLR-4 protein in healthy mice; Changes in expression of TLR-4 protein in M.tb-infected mice before and after curative EDM increased by 17\%, after administration of placebo increased 97\% and decreased 12\% after OAT curative administration; Changes in expression of TLR-4 protein in M.tb-infected mice before preventive administration and after EDM curative administration increased by 20\%, after administration of placebo increased 102\% and decreased by 10\% after the curative administration of OAT. Conclusions: EDM has potential as antituberculosis with TLR-4 regulatory mechanism.

}, keywords = {Antituberculosis., Miana Leaf, TLR-4}, doi = {10.5530/pj.2022.14.63}, author = {Sesilia Rante Pakadang and St. Ratnah and Alfrida Monica Salasa and Jumain and Mochammad Hatta} } @article {1250, title = {The Ability Free Radical Binding of Dengen{\textquoteright}s Stem Bark Extract (Dillenia serrata) From Luwu District Indonesia}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {September 2020}, pages = {1340-1345}, type = {Research Article}, chapter = {1340}, abstract = {

Degenerative diseases such as cancer, heart disease is one of the diseases caused by free radicals. Dengen (Dillenia serrata) is one of Indonesia{\textquoteright}s endemic local plants that has the potential to bind free radicals. So far, only limited to the use of fruit and consumed directly. This study aims to determine the ability of dengen stem bark extract to bind to free radicals expressed in IC50. In the initial research, the total content of polyphenols in dengen stem bark extract was 444.8 mg GAE / g or 44.48\%. Dengen stem bark is extracted by maceration using 70\% ethanol solvent. Antioxidant activity was calculated based on IC50 (Inhibition Concentration) values using the DPPH method, and measured at a wavelength of 516 nm. Ethanol extract of dengen bark is made in 5 concentration series, namely 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. Ethanol extract of dengen bark has a very active antioxidant activity with an IC50 value of 48.33 ppm. Whereas for comparison vitamin C has an IC50 value of 15.448 ppm. From the results, it can be concluded that the ethanol extract of dengen bark has the ability to bind free radicals with IC50 48.33 ppm

}, keywords = {Dengen, Ethanol Bark Extract, Free Radicals, IC50}, doi = {10.5530/pj.2020.12.184}, author = {Santi Sinala and Ismail Ibrahim and Alfrida Monica Salasa} }