@article {2038, title = {Metabolite Profiling of Compounds from Sargassum polycystum using UPLC-QToF-MS/MS}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {June 2023}, pages = {321-333}, type = {Research Article}, chapter = {321}, abstract = {

Background: There are many types of seaweed that have high economic value. Brown seaweed (Sargassum polycystum) can be used as a raw material in the industry and as a medicinal plant. Maintaining the quality of a compound requires an analytical method that can identify the diversity of metabolome profiles. Objective: This investigation seeks to discover the metabolite profile of S. polycystum from Sumenep, Madura Island, Indonesia, utilizing the UPLC-QToF MS/MS equipment. Materials and Methods: The extract was further fractioned using n-hexane, ethyl acetate, and water. The metabolite profiling of extract and fractions used the UPLC-QToF-MS/MS instrument. It was produced with SPE and then introduced into the MS Xevo G2-S QToF detector of the ACQUITY UPLC{\textregistered} H-Class System. The findings of the UPLC-QToF-MS/MS analysis were processed with the MassLynx 4.1 software to obtain chromatogram data and m/z spectra of each observed peak, which were then validated using the ChemSpider and MassBank databases. Results: Based on the results of metabolite profiling using UPLC-QToF-MS/MS, the 96 \% ethanol extract of S. polycystum indicated a total of 61 compounds, the n-hexane fraction indicated a total of 55 compounds, the ethyl acetate fraction indicated a total of 67 compounds, and the water fraction indicated a total of 49 compounds. Conclusion: There are 232 compounds in the extract and a fraction of S. polycystum consisting of 168 known compounds and 64 unknown compounds.

}, keywords = {Metabolite profiling, Sargassum polycystum, Seaweed, UPLC-QToF-MS/MS}, doi = {10.5530/pj.2023.15.82}, author = {Pramudita Riwanti and Intan Kris Prasetyanti and Burhan Ma{\textquoteright}arif} } @article {1325, title = {Analysis of Heavy Metal Contents of Marsilea crenata Presl. Leaves and Soils from East Java Province, Indonesia}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {17-22}, type = {Original Article}, chapter = {17}, abstract = {

Introduction: Marsilea crenata Presl. grows in East Java Province, Indonesia, and showed several pharmacological activities due to its phytoestrogen content. Research indicated its phytoremediation property, and therefore has an impact on heavy metal accumulation in the plants. Lead, cadmium, and mercury are amongst heavy metals that are toxic to the human body. This study aimed to determine and compare Pb, Cd, and Hg mineral contents of soils and leaves of the plants grew in Batu, Pasuruan, Surabaya, and Kediri cities in the province. Method: 10 g of dried powdered leaves and soils were put in separate crucible porcelains, and add 2ml of nitric acid. All were burnt in low heat until white smoke were not formed, and then stood to cool. Ashes obtained were examined using AA 6200 Atomic Absorption Spectrometer (AAS). Result: Showed that leaves samples from the areas showed no Pb and Hg mineral contents that exceeded the permissible limit. Batu City leaf sample had Cd heavy metal content of 2.79 {\textpm} 0.01 ppm, which exceeded the permissible limit. Conclusion: Analysis of heavy metal contents of soils from all of the areas showed no exceed limit of Pb and Hg mineral concentrations. Cd soil contents of Surabaya and Kediri cities, were 0.73 {\textpm} 0.00 and 2.61 {\textpm} 0.08 ppm respectively.

}, keywords = {Aas, Clover, Phytoremediation}, doi = {10.5530/pj.2021.13.3}, author = {Mangestuti Agil and Hening Laswati and Neny Purwitasari and Burhan Ma{\textquoteright}arif} }