@article {1381, title = {Phytochemicals Screening, GC/MS Characterization and Antioxidant Activity of Falcataria moluccana Miq. Barneby and J. W. Grimes Methanolic Extract}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {450-455}, type = {Research Article}, chapter = {450-}, abstract = {

Introduction: In this study, Falcataria moluccana as a plant of West Java community forest was evaluated for its phytochemical content, characterization of secondary metabolites through GC/MS analysis, and antioxidant activity with the DPPH method. Methods: The extraction of F. moluccana twig used maceration with methanol solvent. Phytochemical compounds in F. moluccana methanolic extract were identified using Gas Chromatography-Mass Spectrometry (GC/MS). The antioxidant activity was tested against 2,2-diphenyl1- picrylhydrazyl (DPPH). Results: The phytochemical screening of F. moluccana methanolic extract showed the presence of phenolics, flavonoids, steroids, terpenoids, saponins, and tannins. The results of GC/MS analysis showed that the highest abundance was α-terpinolenic from the terpenoid group with a retention time of 6.776 minutes and a percentage area of 25.85\%. Total phenolic content in methanolic extract of F. moluccana was 145.21 mg GAE/g, total flavonoid was 95.39 mg QE/g while antioxidant activity (IC50) was 12.60 ppm. Conclusion: F. moluccana has potential as natural antioxidant and its active compounds can be developed as pharmaceutical raw materials.

}, keywords = {Antioxidant, Falcataria moluccana, GC/MS, Methanolic extract, Phytochemical}, doi = {10.5530/pj.2021.13.57}, author = {Alfi Rumidatul and I Nyoman Pugeg Aryantha and Endah Sulistyawati} } @article {1366, title = {Production of Secondary Metabolites and its Antibacterial and Antioxidant Activity During the Growth Period of Endophytic Fungi Isolated from Gall Rust Sengon Plants}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {325-331}, type = {Original Article}, chapter = {325}, abstract = {

Introduction: In our previous study, secondary metabolites of endophytic fungi isolated from gall rust sengon showed their bioactive activity against antibacterial and antioxidant, but only gave little effect to antifungal activity. Endophytic fungal culture extracted from gall rust of sengon (Falcataria moluccana Miq. Barneby and J. W. Grimes) has the ability on inhibiting Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. To increase the production of bioactive compounds, this research was focused on isolates which produced the highest activity compounds, and sought the optimal fermentation conditions in the production of bioactive compounds. Methods: The fermentation process was carried out on PDB liquid media for 21 days, under shaker conditions, at room temperature, and sampling measurements were hold every 3 days. Bioassay were carried out against B. subtilis, P. aeruginosa, and E. coli. Results: The early stages fungal growth was adaptation stage, continued by 1-6th days of exponential growth period. Day 6-12th was stationary growth and day 15-21th were cell death periode. The highest secondary metabolite production was achieved at stationary periodes, that was occured at days 9-15th\ and high antibacterial activity was produced on days 9-12th. Conclusion: The best production secondary metabolite that has high activity was at day 9-12th.

}, keywords = {Antibacteri, Antioxidant, Endophytic fungi, Gall rust, Secondary metabolite}, doi = {10.5530/pj.2021.13.42}, author = {Alfi Rumidatul and Noor Rahmawati and Sopandi Sunarya} }