@article {1551, title = {Pharmacokinetic study of phyllanthin and hypophyllanthin after oral administration to rats}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {18th Feb,2014}, pages = {124-130}, type = {Original Article}, chapter = {124}, abstract = {

Objective: The present study was carried out to develop a sensitive and cost effective HPLC method for the determination of bioactive lignans (phyllanthin and hypophyllanthin) and its application in a pharmacokinetic study. Methods: Identification of lignan compounds on C\–18 column was monitored at a range of 199\–400nm using photodiode array detector (PDA) with methanol-water (66:34, v/v) as mobile phase at a flow rate of 1ml/min. Carbamazepine was used as internal standard. Results: From the developed method LOD and LOQ values were found to be 56.14ng/ml and 169.99ng/ml for phyllanthin, and 56.04ng/ml and 169.82ng/ml for hypophyllanthin. The validated RP\–HPLC method herein was applied for pharmacokinetic studies and Cmax (ng/ml) values for administered three oral doses (2.5, 5 and 10mg/kg) of phyllanthin and hypophyllanthin were 0.28\±0.06, 0.53\±0.16, 0.98\±0.22 and 0.68\±0.76, 1.35\±0.23, 2.45\±0.33, respectively. Conclusion: In conclusion, developed HPLC\–PDA method effectively determined the phyllanthin and hypophyllanthin in various solvent and plasma samples. This method was successfully applied in conducting their oral pharmacokinetic studies.

Key words:Phyllanthus amarus, phyllanthin, hypophyllanthin, HPLC{\textendash}PDA, pharmacokinetics.

}, keywords = {HPLC{\textendash}PDA, hypophyllanthin, Pharmacokinetics, Phyllanthin, Phyllanthus amarus}, author = {Madhukiran Parvathaneni, and Ganga Rao Battu, and Ravikumar Jangiti, and Keerthana Diyya} }