@article {1660, title = {Antioxidant Capacity of Chuquiraga Spinosa Less. "Huamanpinta" and Prevention of Carrageenan-Induced Inflammation in Mice}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1287-1296}, type = {Research Article}, chapter = {1287}, abstract = {

Objective: To evaluate the antioxidant capacity of Chuquiraga spinosa extracts and prevention of carrageenan-induced inflammation in mice. Methodology: Experimental design: plant species, erythrocytes and male BALB C53 mice, were considered as biological material. Antioxidant capacity was evaluated in 50\%, 70\%, 96\% and aqueous ethanolic extracts by 2,2-Diphenyl-1-Picrylhydrazyl reduction, malondialdehyde inhibition in oxidized erythrocytes with H2O2 and correlating with polyphenol content equivalent to gallic acid/gram dry extract. Inflammation was evaluated by inoculating carrageenan 2\% in "subcutaneous air bag" of mice: 1) White, 2) carrageenan, 3) dexamethasone 2 mg/kg, 4-6) ethanolic extract 70\% doses 100, 250 and 500 mg/kg respectively; determining nitric oxide, malondialdehyde, total proteins, albumin, leukocytes in exudate and histological changes. Results: Alkaloids, flavonoids, terpenes, phenolic compounds, tannins, carbohydrates, triterpenes, steroids and sesquiterpene lactones were identified; aqueous extract presented greater reduction of 2,2-Diphenyl-1-Picrylhydrazyl (CI50 = 58.99 μg/mL), ethanolic extract 70\% presented greater inhibition of malondialdehyde in erythrocytes (CI50 = 16.44 nm/mL); It was observed that the higher the amount of polyphenols, the greater the reduction of 2,2-Diphenyl-1-Picrylhydrazyl (r=-0.909) and the greater the inhibition of malondialdehyde (r=-0.781). With 500 mg/kg of 70\% ethanolic extract there was greater anti-inflammatory effect inhibiting malondialdehyde, nitric oxide, albumin, total proteins and leukocytes in 55.55\%, 81.92\%, 41.20\%, 31.51\% and 32.45\% (p\<0.01) respectively and less infiltration of leukocytes and lymphocytes in air sac membrane. Conclusion: The extracts of aerial parts of Chuquiraga spinosa showed antioxidant capacity correlated to polyphenol content. The 70\% ethanolic extract prevented inflammation in mice in a dosedependent manner.

}, keywords = {Antioxidant, Ethanolic extract, Leukocytes, Lipoperoxidation, Nitric oxide, Oxidative stress}, doi = {10.5530/pj.2021.13.163}, author = {Hugo Jes{\'u}s Justil-Guerrero and Jorge Luis Arroyo-Acevedo and Juan Pedro Rojas-Armas and Miriam Palomino- Pacheco and Magaly Villena-Tejada and Wilmer Atilio Segura V{\'\i}lchez} }