@article {1884, title = {Inhibitory Effect of Carallia Brachiata Extract Through Regulation of Adipogenesis Pathways in 3T3-L1 Cells}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {655-660}, type = {Research Article}, chapter = {655}, abstract = {

Background: Pharmacological effects of Carallia brachiata Merr. has been reported to show antioxidant effects against the development of diabetes. However, the mechanism underlying antiadipogenic activity have not been investigated. Objective: Effect of Carallia brachiata ethanolic extract was determined on inhibition of adipogenesis in 3T3-L1 adipocytes. Materials and Methods: Adipose tissue development was performed in preadipocyte 3T3-L1 cells culture. Carallia brachiata leaf (CL) and stem (CS) part were selected for measuring cytotoxicity, accumulation of lipids, and genes involved in adipogenic differentiation. Results: During the adipogenic differentiation, CS down-regulated gene expression of adipogenic transcription factors (PPARγ, C/EBPα, aP2, FAS, LPL and SREBP1c). However, CL only suppressed SREBP1c and aP2 genes. The accumulation of lipids was suppressed by CS, but CL could not show this effect. Conclusion: Our findings suggest that ethanol extract of Carallia brachiata stem has a better anti-adipogenesis effect than the leaf part by suppressing adipogenesis-related gene expression. Moreover, inhibition of lipid storage could be decreased insulin resistance risk.

}, keywords = {3T3-L1 adipocytes, Adipogenesis, Carallia brachiata}, doi = {10.5530/pj.2022.14.149}, author = {Linda Chularojmontri and Urarat Nanna and Rawiwun Kaewamatawong and Sudarat Homhual and Wanwisa Suwannaloet} } @article {1678, title = {Effect of Aporosa villosa Stem Ethanolic Extract on Adipogenesis in 3T3-L1 Adipocytes}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1422-1427}, type = {Research Article}, chapter = {1422}, abstract = {

Background: An excessive fat accumulation is related to development of obesity. Obesity is associated with the induction of insulin resistance and diabetes mellitus conditions. Aporosa villosa is a plant that found in the Northern and Northeastern region of Thailand. Objective: The present study used 3T3-L1 adipocytes for investigating the effect of Aporosa villosa stem ethanolic extract (AS) on adipogenesis. Materials and Methods: 3T3-L1 adipocytes were used for measuring the cytotoxicity of AS at a concentration range of 3-100 μg/mL. After adipocyte cells treated with AS (3-100 μg/mL) for 8 days, the lipid accumulation was detected by Oil Red O staining and adipogenic gene expression were determined by quantitative real-time PCR. Results: AS extracts (3-100 μg/mL) did not show cytotoxicity on cell proliferation. After 8 days of treating 3T3-L1 adipocytes with AS at doses of 3, 10, 30 and 100 μg/mL, the lipid droplets were reduced as compared to non-treated cells. Furthermore, the adipogenic genes were measured. The regulators of adipogenesis, CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferatoractivated receptor γ (PPARγ) and sterol regulatory element binding protein 1c (SREBP1c) were found decreasing in AS extracts. The downstream target genes of these regulators cluster of differentiation (CD) 36, fatty acid synthase (FAS) and lipoprotein lipase (LPL) were also reduced by AS treatments. Conclusion: These findings indicate that AS extract has an inhibitory activity on adipogenesis in 3T3-L1 adipocytes via suppressing C/EBPα, PPARγ and SREBP1c.

}, keywords = {Aporosa villosa; Adipogenesis; Obesity}, doi = {10.5530/pj.2021.13.180}, author = {Urarat Nanna and Linda Chularojmontri and Pholawat Tingpej and Rawiwun Kaewamatawong and Sudarat Homhual and Wanwisa Suwannaloet and Thanes Fuangfoo and Jarinyaporn Naowaboot} }