@article {2184, title = {Performance Comparison of Urine Sediment Analytical Tool by Flowcytometry and Digital Imaging with Standardized Manual Microscopic Testing}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {December 2023}, pages = {1189-1196}, type = {Research Article}, chapter = {1189}, abstract = {

Background: Urine sediment examination provides numerous information about patient{\textquoteright}s kidney condition. Flowcytometry and digital imaging system could conduct automatic urine sediment analysis. Therefore, determining the diagnostic performance of the examination is very important in the management carried out by the clinician. This study aims to determine the diagnostic performance of urine flowcytometry and digital imaging sediment examination tools compared to manual microscopes that are standardized as gold standards. Methods: This study is an analytical observational study with a cross-sectional approach on 92 urine samples of patients who attended the Internal Medicine Nephrology Polyclinic of Dr. Soetomo Academic Hospital Surabaya. The performance of flowcytometry and digital imaging methods is assessed by calculating sensitivity and specificity. Analysis of the suitability of each urine sediment parameter used Cohen{\textquoteright}s kappa. Urine sediment analysis with a light microscope was conducted with the Shih-Yung method as a gold standard. Results: The erythrocyte parameters revealed a very favorable result with the concordance of the flowcytometry with the Shih-Yung method (κ=0.82) and fair results for WBC (κ=0.25), Epithelium (κ=0.57) and Cast (κ=0.27). At the same time, yeast had substantial conformity (κ=0.63). The digital imaging method showed substantive fit for WBC (κ=0.676), RBC (κ=0.621), fair for SEC (κ=0.42) and NSE (κ=0.24), moderate for Yeast (κ=0.45), and slight for Hyaline Cast (κ=0.074) and Path Cast (κ= 0.134) Conclusion: The urine flowcytometry demonstrates better performance compatibility with a standardized manual microscope compared to urine digital imaging. However, pathological samples should still be verified with a manual microscope

}, keywords = {Digital imaging, Flowcytometry, Microscopic, Urine sediment}, doi = {10.5530/pj.2023.15.216}, author = {Nur Fitri Hayati Melida Ritonga and Ferdy R. Marpaung and Hartono Kahar and Nunuk Mardiana and Yessy Puspitasari} } @article {1856, title = {Nephrotic Syndrome with Focal Segmental Glomerulosclerosis Histological Feature: A Case Report}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {459-461}, type = {Case Report}, chapter = {459}, abstract = {

Introduction: Nephrotic syndrome is a clinical syndrome of heavy proteinuria and hypoalbuminemia or hypoproteinemia. Renal biopsy is fundamental to assess not only the type but also the degree of disease activity. The overall prognosis and response to treatment often depend on the severity of histological lesions and their reversibility.1,2 Case Presentation: An eighteen years old man with nephrotic syndrome and planned for a kidney biopsy. This case showed a patient with swollen face (especially on the cheek). On Biopsy results showing the glomerulus proliferation of cells and mesangeal matrix, adhesions in (50\%) glomerulus, focal sclerosis in some glomeruli, erythrocyte cells visible in the urinary space and thickening of the basement membrane in some glomeruli, partially atrophic tubules, visible erythrocytes in the tubular lumen. Conclusion: An eighteen years old man with nephrotic syndrome with cushing syndrome and hypokalemia who had a renal biopsy. The histological feature from the renal biopsy was focal segmental glomerulosclerosis. The underlying cause of FSGS is still unclear. Assessing the diagnosis and etiology become important to direct the subsequent clinical approach and therapy

}, keywords = {Focal segmental glomerulosclerosis, Nephrotic syndrome, Renal biopsy}, doi = {10.5530/pj.2022.14.122}, author = {Dian Samudra and Widodo and Nunuk Mardiana and Artaria Tjempakasari and Pranawa and Aditiawardana and Anny Setijo Rahaju} }