@article {1895, title = {Cichorioside a biocoumarin modulates lipid and glucose storage on 3T3-L1 cell lines: In vitro and in silico approach}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {933-937}, type = {Original Article }, chapter = {933}, abstract = {

Natural coumarins are reputed to demonstrate several biological effects to improve adverse health conditions including; obesity. This study was conducted to explore the potential in vitro lipid and glucose modulatory activity of cichorioside on 3T3-L1 cell lines . Methods: The 3T3-L1 cell lines were cultured and cell viability was assessed. Glucose content in medium of cultured cells was measured. Differentiation of 3T3-L1 cells from pre-adipocytes to adipocytes was evaluated upon addition of cichorioside. Expression of mRNA of the peroxisome proliferator-activated receptor gamma (PPARγ) was estimated. Results: Survival of around 90\% of cultured cells was observed at 10μM cichorioside. Cichorioside inhibited glucose uptake from the medium by the 3T3-L1 cell lines. Cichorioside considerably inhibited pre-adipocyte differentiation and the lipid content in intercellular storage. Cichorioside demonstrated an upregulation of the mRNA expression of PPARγ. Moreover, the docking studies supported the results via the deep interaction of cichorioside with amino acids residue of PPAR-γ. Taken together, these findings are the first report on in vitro evaluation of cichorioside to modulate the lipid storage and glucose uptake of cultured 3T3-L1 cell lines

}, keywords = {3T3-L1cell lines, Cichorioside, Oil red O staining, PPARγ}, doi = {10.5530/pj.2022.14.192}, author = {Shahad Mohammed Nasser Alqahtani and Dalya Ramzi Alsuliman and Abdullah Jalal Alasoom and Hussein Ali Burshed and Marwan Mohamed Alshawush and Abdullah Abdulhamid Altaweel and Hany Ezzat Khalil} } @article {1890, title = {Profound Assessment of Phytochemical, Botanical and Antioxidant Characteristics Including Determination of Total Phenolic and Flavonoid Contents of Stem Bark of Cordia obliqua L.}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {753-758}, type = {Original Article }, chapter = {753}, abstract = {

Cordia obliqua is known as Bumber. Its fruits and leaves are popularly used because of antioxidant and anti-diabetic activities. The purpose of this study is to evaluate the phytochemicals and antioxidant activity as well as botanical features of stem bark of Cordia obliqua. Chemical components were preliminary screened in various fractions based on the polarities including; n-hexane, chloroform, ethyl acetate, butanol and aqueous fractions of using standard procedures. Total phenolic (TPC) and flavonoid (TFC) contents were assessed by Folin-Ciocalteau and aluminium chloride methods respectively. The antioxidant activity was evaluated by ABTS antioxidant procedure, using ascorbic acid as standard. Results confirmed that stem bark of Cordia obliqua chemically is characterized by the presences of flavonoids, alkaloids, steroids, saponins, tannins and carbohydrates at different levels in various fractions and the absence of cardiac glycosides and anthraquinones. Microscopically, the plant is characterized by presence of big Ca oxalate clusters, various types of xylem vessels and big amount of cork cells. TPC was ranged from 13.6{\textpm}1.4 and 220.5{\textpm}3.4 mg GAE/g dry plant extract and TFC was ranged from 0.029{\textpm}0.12and 15.46{\textpm}0.33 mg QE/g dry plant extract. Due to the high phenolic and flavonoid content in butanol and ethyl acetate fractions; results of antioxidant using ABTS assay showed high antioxidant activity with IC50 valued 11.84{\textpm}1.2 μg/ ml for butanol fraction and 14.81{\textpm}1.1 μg/ml for ethyl acetate fraction. Taken together, the research work demonstrated the potential natural antioxidant value of the waste product stem bark of Cordia obliqua. The study endorses forthcoming work to isolate and identify the chemical constituents in stem bark of Cordia obliqua.

}, keywords = {ABTS., Boraginaceae, Cordia obliqua, DPPH, Folin-Ciocalteau, Total phenolic and flavonoid}, doi = {10.5530/pj.2022.14.163}, author = {Dalya Ramzi Alsuliman and Zainab Mohammed Al Sultan and Maryam Khalil Almajhad and Nour Kamal Alhajri and Abdullah Abdulhamid Altaweel and Abdullah Jalal Alasoom and Hany Ezzat Khalil} }