@article {1895, title = {Cichorioside a biocoumarin modulates lipid and glucose storage on 3T3-L1 cell lines: In vitro and in silico approach}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {933-937}, type = {Original Article }, chapter = {933}, abstract = {

Natural coumarins are reputed to demonstrate several biological effects to improve adverse health conditions including; obesity. This study was conducted to explore the potential in vitro lipid and glucose modulatory activity of cichorioside on 3T3-L1 cell lines . Methods: The 3T3-L1 cell lines were cultured and cell viability was assessed. Glucose content in medium of cultured cells was measured. Differentiation of 3T3-L1 cells from pre-adipocytes to adipocytes was evaluated upon addition of cichorioside. Expression of mRNA of the peroxisome proliferator-activated receptor gamma (PPARγ) was estimated. Results: Survival of around 90\% of cultured cells was observed at 10μM cichorioside. Cichorioside inhibited glucose uptake from the medium by the 3T3-L1 cell lines. Cichorioside considerably inhibited pre-adipocyte differentiation and the lipid content in intercellular storage. Cichorioside demonstrated an upregulation of the mRNA expression of PPARγ. Moreover, the docking studies supported the results via the deep interaction of cichorioside with amino acids residue of PPAR-γ. Taken together, these findings are the first report on in vitro evaluation of cichorioside to modulate the lipid storage and glucose uptake of cultured 3T3-L1 cell lines

}, keywords = {3T3-L1cell lines, Cichorioside, Oil red O staining, PPARγ}, doi = {10.5530/pj.2022.14.192}, author = {Shahad Mohammed Nasser Alqahtani and Dalya Ramzi Alsuliman and Abdullah Jalal Alasoom and Hussein Ali Burshed and Marwan Mohamed Alshawush and Abdullah Abdulhamid Altaweel and Hany Ezzat Khalil} } @article {1894, title = {Phytochemical Approach Including Total Phenolic and Flavonoid Contents and Evaluation of in vitro ABTS Antioxidant Capacity and Lipoxygenase Inhibition of Anisosciadium lanatum}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {928-932}, type = {Original Article }, chapter = {928}, abstract = {

Anisosciadium lanatum Boiss is commonly known in Bedouins as besbas. Traditional, it is palatable plant and used in medicine of livestock to treat skin conditions . The main objective of current approach was to screen the presence of different kind of metabolites applying standard procedures followed by assessment of total phenolic (TPC) and flavonoids (TFC) contents. In addition, the in vitro ABTS antioxidant and lipoxygenase activities were evaluated. Different organs (leaves, stems and flowers) of Anisosciadium lanatum were extracted using 70\% methanol to yield total methanol extracts of leaves (TML), stems (TMS) and flowers (TMF). Results demonstrated that TML, TMS and TMF are characterized by the content of different constituents such as flavonoids, phenolics/tannins, steroids, saponins, and carbohydrates at different levels. Ethyl acetate (EA) and butanol (BT) fractions of TML and TMS demonstrated the highest percentage of TPC and TFC. The results demonstrated the competence of EA and BT as free radical scavenger fractions compared to other fractions and its opportunity to contain bioactive antioxidant metabolites . TML, TMS and TMF exhibited Lipoxygenase inhibitory activity with IC50 values of 4.88, 5.40 and 6.05 μg/mL, respectively when compared to that of the positive control baicalein (IC50: 0.27 μg /mL). In conclusion, present investigation highlighted the potential of Anisosciadium lanatum to be promising candidate with activity against wide range of inflammatory-related diseases.

}, keywords = {ABTS, Anisosciadium lanatum, Lipoxygenase, total flavonoid, total phenolic}, doi = {10.5530/pj.2022.14.191}, author = {Mohammed Wasel Matar and Shahad Mohammed Nasser Alqahtani and Duaa Adnan Alghafli and Abdullah Abdulhamid Altaweel and Abdullah Jalal Alasoom and Hussein Ali Burshed and Marwan Mohamed Alshawush and Hany Ezzat Khalil} }