@article {2331, title = {Vaccinium corymbosum: Phenolic Compound Content and Effect of Fruit Extract on Blood Glucose in Healthy Mice}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {August 2024}, pages = {716-725}, type = {Original Article}, chapter = {716}, abstract = {

Introduction: In the context of the increasing prevalence of metabolic diseases such as diabetes, the search for natural compounds with potential impact on glycemic regulation has become a crucial area of research. Among the numerous options available, Vaccinium corymbosum extract, commonly known as "blueberry", has emerged as a promising candidate due to its rich composition of phytochemicals with antioxidant, anti-inflammatory and hypoglycemic properties. The aim of this study was to determine the total phenolic content (TPC) and the activity of Vaccinium corymbosum ("blueberry") fruit extract on glycemia in healthy mice. Methods: The Folin-Ciocalteau method was applied in order to quantify the phenolic compounds and the BE was administered to 25 mice distributed in six groups: control, negative control, experimental-D1- D2-D3, which were administered the BE in doses of 40, 80 and 120 mg/kg b.w. respectively; and insulin group; which were subjected to the glucose tolerance test (GTT) taking blood samples after 30, 60, 120 and 180 minutes. Results: The total phenolic content (TPC) amount found in the berries was 3.79{\textpm}0.06 GAE/dry weight (mg/g) and 18.96{\textpm}0.28 GAE/solution (mg/L). Statistically significant differences were observed between the three doses of BE and the negative control during GTT as well as induced a significant reduction in area under the curve (AUC) compared to the negative control. Conclusions: the three doses of the BE decreased glucose levels being the dose of 40 mg/kg b.w. the one that produced a statistically significant decrease with respect to the doses of 80 and 120 mg/kg b.w. during GTT.

}, keywords = {Animal studies, Blueberry, Hypoglycemic Effect, Insulin, Phenols, Type 2 Diabetes.}, doi = {10.5530/pj.2024.16.121}, author = {Susana Rubio-Guevara and Olga Castillo-Medina and Marleni Villacorta-Zavaleta and Marleni Villacorta-Zavaleta and Dan Altamirano-Sarmiento and Elena Caceres-Andonaire and Matilde Farias and Nayly Chinchay and Claudia Guerrero and Josue Flores and Edgar Vilela and Sidny Nunez and Janina Sernaque and Felipe Pacherres and Gabriela Mena and Maria Trillo and Julio Amayo and Karyn Olascuaga-Castillo} } @article {2119, title = {Phytochemical Screening and Antiinflammatory Activity of the Extract from the Leaves of Desmodium molliculum (Kunth) DC (Fabaceae) in Rats with Acute Inflammation}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {786-790}, type = {Original Article}, chapter = {786}, abstract = {

Inflammation and pain are the initial response mechanisms to environmental aggression on the human body. The traditional use of plants such as Desmodium Molliculum (Kunth) DC, among the Peruvian population for the treatment of inflammatory diseases, has occurred since ancient times. The objective of this research was to determine the presence of secondary metabolites and evaluate the antiinflammatory activity of Desmodium molliculum (EDM) leaves in rats with acute inflammation induced using carrageenan. The phytochemical profile was performed for the main secondary metabolites with biological activity. Subsequently, 25 rats were divided into 5 groups and treated as follows: Group I and II: Physiological Saline Solution (PSS) by oral administration. Group III: Sodium Diclofenac (25 mg/kg body weight) by intraperitoneal administration. Group IV and V: EDM at 250 mg/kg bw and 500 mg/kg bw by oral administration, respectively; 30 minutes after administration, acute inflammation was induced in Groups II, III, IV, and V using the subplantar edema technique with 1\% w/v carrageenan. The volume displaced by the hind paw was evaluated in all 5 groups using a digital plethysmometer every 60 minutes for 5 hours. The results were obtained from the displaced volume (Mean {\textpm} SD), with the most representative values obtained at 240 minutes, where EDM at 250 mg/kg (0.57 {\textpm} 0.07 ml) bw and 500 mg/kg bw (0.578 {\textpm} 0.051 ml) showed significant anti-inflammatory activity (ANOVA p\<0.05). We concluded that Desmodium Molliculum has anti-inflammatory activity at doses of 250 mg/kg bw and 500 mg/kg bw.

}, keywords = {Acute inflammation, Carrageenan, Desmodium, Dog{\textquoteright}s Paw, Edema Subplantar, Fabaceae.}, doi = {10.5530/pj.2023.15.153}, author = {Karyn Olascuaga-Castillo and Olga Castillo-Medina and Marleni Villacorta-Zavaleta and Dan Altamirano- Sarmiento and Elena Caceres-Andonaire and Maria Llontop and Fatima Malca and Sebastian Noe and Cyntia Blanco-Olano} }