@article {478, title = {Composition and Antibacterial Activity of Hydro-Alcohol and Aqueous Extracts Obtained from the Lamiaceae Family}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {December 2017}, pages = {81-91}, type = {Original Article}, chapter = {81}, abstract = {

Context: Plant from the Lamiaceae family are considered as dietary spices and medicinal herbs traditionally used in medicine for the treatment of several pathologies. Objective: Evaluation of the in vitro antibacterial activity of ethanol and aqueous extracts of nine Moroccan plants from the Lamiaceae family against six bacterial strains regularly implicated in toxiinfection. Method: The antibacterial activities of hot (HAE), cold (CAE) aqueous extracts and ethanolic extracts (EE) were evaluated using agar-well diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and IC50 against six foodborne bacteria (Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica). Total phenolic and flavonoid contents were assessed as well. Results: In the present study, the ethanol extracts were rich in polyphenols, with middling values of flavonoids, and relatively poor in condensed tannins. The presence of saponins, essential oils, irroides, alkaloids, anthocyanins, and aldehydes was recorded. The effect of the extracts was directly bactericidal for B. cereus and L. monocytogenes; for the other strains, the MBC value was twice higher than that of MIC. However, the ethanol extract (EE) of O. majorana and T. satureioides showed the highest antibacterial activity. With the principal component analysis, it was demonstrated that the nine Lamiaceae family plants possess a powerful antibacterial effect, correlated with their phenolic content. Statistical analysis: Analysis of variance was performed by uni-varied ANOVA in the software SPSS 22 Fr. Conclusion: The active compounds were thermostable and soluble in ethanol and water. The antimicrobial activities of the plants extracts investigated may contribute to understand their involvement in traditional medicine against many microbial infections.

}, keywords = {Antibacterial activity, Aqueous Extract., Flavonoic Content, Hydro-Alcohol Extract, Lamiaceae Family, Phenolic compounds}, doi = {10.5530/pj.2018.1.16}, url = {http://fulltxt.org/article/402}, author = {Ramdan Btissam and El Malki Fatima and Eddarraji Kamal and Greche Hassane and NHIRI Mohamed} } @article {558, title = {In vitro Study of Antibacterial Activity of Hydro-Alcohol Morrocan Plants Extracts}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {519-526}, type = {Original Article}, chapter = {519}, abstract = {

Context: Several aromatic and medicinal Morrocan plants have been used traditionally in pharmaceutical products and traditional medicine for the treatment of several pathologies. Objective: Evaluation of the protective power of nine Moroccan plants ethanol extracts against some strains of bacteria. Method: The antibacterial activities of ethanolic extracts (EE) were evaluated using agar-well diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and IC50 against nine foodborne bacteria [Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus (PN15 and 25923), Escherichia coli (TF2 and ATCC 25929), Pseudomonas aeruginosa (P116 and 195) and Salmonella enterica]. Screening of chemical constituents was carried out as well. Results: We noted the presence of leuco-anthocyanins, anthocyanins, essential oils, alkaloids, and aldehydes in the extracts. The strains of S. enterica followed by S. aureus and P. aeruginosa were the most resistants to the extracts effect. The principal component analysis (APC) demonstrated that the highest antibacterial activity was that of L. nobilis and O. europaea ethanol extract (EE), which was directly bactericidal on all the strains tested with the exception of P. aeruginosa. While, R. tinctorum, S. indicum and L. sativum were characterized by the lowest activity. Statistical analysis: Analysis of variance was performed by uni-varied ANOVA in the software SPSS 22 Fr. Conclusion: The active compounds were soluble in ethanol. The antimicrobial activities of L. nobilis and O. europaea may contribute to understand their involvement in pharmaceutical products and traditional medicine against many microbial infections.

}, keywords = {Antibacterial activity, Chemical composition, Hydro-Alcohol Extract}, doi = {10.5530/pj.2018.3.85}, url = {http://fulltxt.org/article/518}, author = {Ramdan Btissam and E I Malki Fatima and NHIRI Mohamed} } @article {45, title = {In vitro study of anti-glycation and radical scavenging activities of the essential oils of three plants from Morocco: Origanum compactum, Rosmarinus officinalis and Pelargonium asperum}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Mar-Apr 2015}, pages = {124-135}, type = {Original Article}, chapter = {124}, abstract = {

Introduction: We have carried out our study on the chemical composition; anti-glycation andradical scavenging activities of Origanum compactum, Rosmarinus officinalis and Pelargonium asperum essential oils (EO) that were harvested in the northeast of Morocco. These plants have been traditionally used in medicine as a chief ingredient of many polyherbal formulations for the treatment of several pathologies. Method: The phytochemical study was revealed by GC-MS. The protein glycation inhibitory activity of EO extracted from these plant tissues was evaluated in vitro using the model system of bovine serum albumin and methylglyoxal. The measure of DPPH\• radical reducing power was used to evaluate the antiradical activity. To test each fraction, we used the IC50 value previously obtained for the crude oils. Results: The phytochemical study of these essential oils showed that p-Thymol, Eucalyptol and Citronellal were respectively the major components in the three investigated EO of O.compactum, R. officinal is and P. asperum. More than 90\% of the total components were detected. The extracts and fractions with glycation inhibitory activity also showed antiradical activity when the DPPH\• radical reducing power was measured. Conclusion: The glycation inhibitory activity was correlated with the antiradical potency of the extracts. Thus, the positive glycation inhibitory and antiradical activities of these plants might suggest a possible role in targeting aging and diabetic complications.The presence of various bioactive compounds confirms the application of these plants for various diseases by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug.

}, keywords = {Aging, Antiglycation, Antioxidant, Diabetes, Essential oil, Radical scavenger}, doi = {10.5530/pj.2015.2.7}, author = {Ramdan Btissam and Amakran Amina and NHIRI Mohamed}, editor = {Ramdan Rajae} } @article {1445, title = {In vitro study of anti-glycation and radical scavenging activities of the essential oils of three plants from Morocco: Origanum compactum, Rosmarinus officinalis and Pelargonium asperum}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {8th Jan, 2015}, pages = {124-135}, type = {Original Article}, chapter = {124}, abstract = {

Introduction: We have carried out our study on the chemical composition; anti-glycation andradical scavenging activities of Origanum compactum, Rosmarinus officinalis and Pelargonium asperum essential oils (EO) that were harvested in the northeast of Morocco. These plants have been traditionally used in medicine as a chief ingredient of many polyherbal formulations for the treatment of several pathologies. Method: The phytochemical study was revealed by GC-MS. The protein glycation inhibitory activity of EO extracted from these plant tissues was evaluated in vitro using the model system of bovine serum albumin and methylglyoxal. The measure of DPPH\• radical reducing power was used to evaluate the antiradical activity. To test each fraction, we used the IC50 value previously obtained for the crude oils. Results: The phytochemical study of these essential oils showed that p-Thymol, Eucalyptol and Citronellal were respectively the major components in the three investigated EO of O.compactum, R. officinal is and P. asperum. More than 90\% of the total components were detected. The extracts and fractions with glycation inhibitory activity also showed antiradical activity when the DPPH\• radical reducing power was measured. Conclusion: The glycation inhibitory activity was correlated with the antiradical potency of the extracts. Thus, the positive glycation inhibitory and antiradical activities of these plants might suggest a possible role in targeting aging and diabetic complications.The presence of various bioactive compounds confirms the application of these plants for various diseases by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug.

Key words: Aging, Antioxidant, Antiglycation, Diabetes, Essential oil, Radical scavenger.

}, keywords = {Aging, Antiglycation, Antioxidant, Diabetes, Essential oil, radical scavenger.}, author = {Ramdan Btissam and Ramdan Raja and Amakran Amina and Vannier Brigitte and NHIRI Mohamed} }