@article {211, title = {Antioxidant and Hypoglycemic Activities of Clausena anisata (Willd.) Hook F. Ex Benth. Root Mediated Synthesized Silver Nanoparticles}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {September 2016}, pages = {579-586}, type = {Original Article}, chapter = {579}, abstract = {

The present study was carried out to examine the hypoglycemic effect of Silver Nanoparticles (SNPs) using ethanolic root extract of Clausena anisata (Willd.) Hook. f. ex Benth. A cost effective and eco friendly technique for green synthesis of SNPs from 1mM AgNO3 solution through the root extract was carried out. The biosynthesized SNPs were characterized using UV spectrophotometry, Field Emission Scanning Electron Microscopy (FESEM), X-ray Diffraction analysis (XRD), Energy Dispersive Spectroscopy (EDS) and Fourier transform infra-red (FTIR) spectrometry. DPPH assay was done to determine the antioxidant activity and for in vitro hypoglycemic activity glucose uptake by yeast cells, alpha amylase inhibition assay, adsorption capacity and glucose diffusion assay was performed. The SNPs of ethanolic root extract were found to be spherical in shape and up to 32.75 nm average in size. They showed alpha amylase inhibitory activity of 83.60\% at 500 g/ml. The glucose uptake by yeast cells was found to increase with an increase in concentration. The maximum glucose uptake was found to be 69.51\% at 10mM concentration. The molar concentration of glucose was directly proportional to the glucose binding capacity of extracts.The rate of glucose diffusion across the membrane was found to increase from 30 to 180 minutes. The DPPH scavenging activity was found to be potent (74.07\%) at 500 g/ml. The hypoglycemic effect exhibited by the SNPs was revealed by simple in vitro model of yeast cells, mediated by glucose adsorption, increasing glucose diffusion and glucose transport across the cell membrane.

}, keywords = {antioxidant activity, C. anisata, Diabetes, Glucose and Silver nitrate, Silvernanoparticles}, doi = {10.5530/pj.2016.6.10}, author = {Arsia Tarnam Yakoob and Nargis Begum Tajuddin and Muhammad Ilyas Mohammed Hussain and Shilu Mathew and Archunan Govindaraju and Ishtiaq Qadri} } @article {210, title = {Gc-Ms Analysis of Ethanolic Stem Extract of Clausena anisata (Willd.) Hook F Ex Benth}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {September 2016}, pages = {576-578}, type = {Original Article}, chapter = {576}, abstract = {

Objective: In the present study, the bioactive components present in the ethanol stem extract of Clausena anisata was analyzed by using Gas Chromatography Mass Spectrometry analysis technique (GC-MS). Clausena anisata, a medicinal plant belonging to the family Rutaceae, is represented by 20 species available in India and used traditionally for the treatment of several ailments but there is a requirement to identify its phytoconstituents, its target, mode of action and treatment using plant products either alone or in combination with synthetic drugs. Methods: Clausena anisata stem was procured from Manamettupatti, a village of Pudukottai District, Tamil Nadu. The shade dried stem was powdered and extracted using ethanol by maceration method. One microlitre of the extract was subjected to GC-MS analysis to detect the presence of bioactive compounds present in the stem of C. anisata. Results:The results showed that the ethanol stem extract of C. anisata contained nine bioactive compounds, of which the major one is n-hexadecanoic acid (78.54\%), followed by 8-octadecenoic acid, methyl ester, [E]- (6.638\%). The total number of compounds obtained was compared with National Institutes of Standard and Technology (NIST) library that contains more than 62,000 known compounds based on retention time and molecular mass. Conclusion: In this study, nearly nine compounds have been identified from the ethanolic stem extract of C. anisata using GC-MS analysis which was mainly composed of fatty acids and sterols. The GC-MS analysis is used to understand the nature of active principles present in this plant revealed that the plant can be used as a potential source of new useful drugs.

}, keywords = {C. anisata, Ethanol, GC-MS analysis, maceration and phytoconstituents}, doi = {10.5530/pj.2016.6.9}, author = {Arsia Tarnam Yakoob and Nargis Begum Tajuddin and Shilu Mathew and Muhammad Ilyas Mohammed Hussain and Ishtiaq Qadri} }