@article {219, title = {Cytotoxicity of Fucoidan from Three Tropical Brown Algae Against Breast and Colon Cancer Cell Lines}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {14-20}, type = {Original Article}, chapter = {14}, abstract = {

Introduction: Fucoidan is a sulfated polysaccharide that has a wide range of bioactivities including anti-cancer. This polysaccharide commonly is extracted from marine brown seaweed. There is lack of information on the fucoidan extracted from tropical brown algae and its anti-cancer activity. Objectives: The objectives of this study were to purify fucoidan from Sargassum sp., Turbinaria sp. and Padina sp., and to evaluate their cytotoxicity against breast cancer (MCF-7) and colon cancer cells (WiDr). Materials and Methods: Fucoidan extraction was conducted by using acid extraction method. Purified fucoidans were obtained by DEAE cellulose column chromatography and confirmed by HPLC and FT-IR spectrometry. The cytotoxicity was evaluated by using the MTT (3-[4,5-dimethylthiazol-2-yl] -2,5- diphenyltetrazolium bromide) assay. Results: Fucoidan from Sargassum sp. and Turbinaria sp. showed low cytotoxicity with IC50 ranging between 461-663 \μg/mL. Higher cytotoxicity against MCF-7 and WiDr was showed by fucoidan from Padina sp. with IC50 of 144 and 118 \μg/mL, respectively. While its IC50 against Vero cells was 501 \μg/mL.Standard fucoidan from Fucus vesiculosus exhibited IC50 of 60, 63 and 211 \μg/mL against MCF-7, WiDr and Vero Cells, respectively. Although the IC50 was higher than that of standard fucoidan, Padina sp. fucoidan showed cytotoxicity comparable with standard fucoidan at concentrations below 100 \μg/mL. Conclusion: These results indicated that Padina sp.fucoidan showed potential selective cytotoxicity, and promising for the development of an anti-cancer compound.

}, keywords = {Breast cancer, Colon cancer, Cytotoxicity., Fucoidan, Phaeophyta}, doi = {10.5530/pj.2017.1.3}, author = {Alim Isnansetyo and Fadilah Nor Laili Lutfia and Muhammad Nursid and Trijoko and Ratna Asmah Susidarti} }