@article {267, title = {ACE Inhibitory Activity, Total Phenolic and Flavonoid Content of Pereskia saccharose Griseb. Leaves Extract}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {285-287}, type = {Original Article}, chapter = {285}, abstract = {

Introduction: Angiotensin-converting enzyme inhibitors (ACEi) are drugs that can control hypertension. Pereskia saccharose Griseb. leaves have been used traditionally as antihypertensive. Objective: The objective of this study was to determine the antihypertensive activity through inhibition of ACE activity, the total phenolic content and total flavonoid content of the ethanolic extract of Pereskia saccharose Griseb. leaves and its fractions. Methods: Extraction was done by maceration with 80\% ethanol and fractionation performed by liquid-liquid partition. Results: In vitro ACE inhibitory activity assay of the ethanolic extract using ACE Kit-WST Dojindo had IC50 value of 3.448 \μg/mL and ethyl acetate fraction had IC50 value of 1.714 x 10-3 \μg/mL. Ethyl acetate contained the highest amounts of both TPC (72.991 \± 0.932 mg GAE/g sample) and TFC (61.337 \± 1.612 mg QE/g sample). Conclusion: The results suggest that Pereskia saccharose Griseb. possess ACE inhibitory activity.

}, keywords = {ACE inhibitor, Flavonoid, Pereskia saccharose Griseb, phenolic}, doi = {10.5530/pj.2017.2.49}, url = {http://phcogj.com/fulltext/316}, author = {Sarlina Jihan Lusiyanti and Katrin and Rissyelly and Nuraini Puspitasari and Putu Gita Maya Widyaswari Mahayasih} } @article {261, title = {Inhibition Activity of Angiotensin Converting Enzyme (ACE) and Determination of Total Phenolic and Flavonoid Compound from Bitter Melon Leaves (Momordica charantia L.)}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {252-256}, type = {Original Article}, chapter = {252}, abstract = {

Introduction: Hypertension is a leading problem in Indonesia, because of the high prevalence and the long term effect. Bitter melon leaves (Momordica charantia L.) has been traditionally utilized to treat hypertension, yet not many studies explained the antihypertension effect of this plant. Methods: This study was conducted to test the benefit of bitter melon leaves as antihypertension using angiotensin converting enzyme inhibition activity in 80\% ethanolic extract and its fractions (n-hexane, ethyl acetate, and n-butanol) using ACE kit-WST (Dojindo, Japan). Then the antihypertension activity was associated with the phenolic content which was expressed in gallic acid equivalent (GAE) and total flavonoid in quercetin equivalent (QE). Results: Result of the study showed that the IC50 value obtained from the ethanolic extract to inhibit ACE activity was 7.52 \μg/mL and the highest inhibition obtained in ethyl acetate fraction with IC50 value was 4,29 \μg/mL. Phenolic total and flavonoid total determination also showed that the highest content obtained in ethyl acetate fraction with 18.752 mg GAE/gr extract and 8.310 mg QE/gr extract, respectively. Conclusion: According to the study, it could be concluded that bitter melon (Momordica charantia L.) leaves provide inhibition activity against Angiotensin Converting Enzyme (ACE) and chemical compounds that were expected to play an active role in inhibiting ACE were flavonoid and tannin.

}, keywords = {Angiotensin converting enzyme, antihypertension, Momordica charantia L., total flavonoid., total phenolic}, doi = {10.5530/pj.2017.2.43}, url = {http://phcogj.com/fulltext/310}, author = {Putri Lestari and Katrin and Rissyelly and Putu Gita Maya Widyaswari Mahayasih} }