@article {1264, title = {Expression of Heat-labile Enterotoxin of Escherichia coli in Biolistic Transformed Hairy Roots of Daucus carota L.}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {September 2020}, pages = {1440-1443}, type = {Research Article}, chapter = {1440}, abstract = {

Expression and secretion of recombination proteins in transgenic hairy roots have opened door to simple, feasible and economic option compared to animal and bacterial systems. Here, we report a feasibility study for producing the nontoxic B subunit of Escherichia coli heatlabile enterotoxin (LTB) in Daucus carota L hairy root where transformation is achieved by biolistic gene gun method. Agrobacterium rhizogenes strain (R-1000) cells containing synthetic LTB gene in pMYO51T plant expression vector under the CaMV 35S promoter introduced to Daucus carota L roots by biolistic gene gun method. PCR amplification confirmed the integration of synthetic LTB gene in carrot hairy roots. Western blot analysis confirmed production of LTB pentamer in similar banding pattern to the native bacterial derived LTB. 0.50-0.70\% of LTB protein estimated in carrot hairy roots. GM1-ganglioside assay indicated formation of biological active pentamers. LTB protein with biochemical properties identical to native LTB protein in the hairy roots of edible carrot roots opens the way for inexpensive, safe, and effective plant-based edible vaccines for humans and animals.

}, keywords = {Carrot, Gene gun, hairy root, LTB}, doi = {10.5530/pj.2020.12.198}, author = {Sharada Laxman Deore and Someshekhar S Khadabadi and Bhushan A Baviskar} } @article {231, title = {Development and Evaluation of Herbal Sunscreen}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {83-97}, type = {Original Article}, chapter = {83}, abstract = {

Thus present research work deals with the development and evaluation of topical photo protective formulation, containing antioxidant, wound healing, anti-inflammatory and rather photo protective poly phenols like curcumin, quercetin, resveratrol and safranal. The present research work provides stable natural photo protective formulation with antioxidant potential, high SPF and more important uniform UVA/UVB protection.

}, keywords = {Curcumin, Quercetin, Resveratrol, safranal, SPF., Sunscreen}, doi = {10.5530/pj.2017.1.15}, author = {Mukund Manikrao Donglikar and Sharada Laxman Deore} } @article {144, title = {Sunscreens: A review}, journal = {Pharmacognosy Journals}, volume = {8}, year = {2016}, month = {January 2016}, pages = {171-179}, type = {Review Article}, chapter = {171}, abstract = {

Sunlight despite of source of life and energy creating major health challenges like sunburn, pigmentation, wrinkles, dermatitis, urticaria, ageing, immune-suppression and number of skin cancers too. Sun protective clothes and or sunglasses provide insufficient and less convenient approach to get rid of all these health hazards. So sunscreen protection is popular mean among various regions of world. Present article have summarize types and classification, regulations, terminologies, evaluation methods, labeling, dosage and controversies of sunscreens. Natural chemical classes like phenolics (tannins, flavonoids), carotenoids, vitamins, oils are also discussed.

}, keywords = {Antioxidant., COLIPA, IPD, ISO, Polyphenols, PPD, SPF, UV rays}, doi = {10.5530/pj.2016.3.1}, author = {Mukund Manikrao Donglikar and Sharada Laxman Deore} } @article {81, title = {Comparative Pharmacognostic, Phytochemical and Biological evaluation between five Chlorophytum species}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {317-325}, type = {Original Article}, chapter = {317}, abstract = {

Objective: To establish comparative pharmacognostic, phytochemical and biological evaluation parameters between five Chlorophytum species i.e. Chlorophytum borivilianum Santapau and Fernades, Chlorophytum comosum (Thunb.) Jacq., Chlorophytum tuberosum Br., Chlorophytum laxum R. Br. and Chlorophytum arundinaceum Baker, of very popular Ayurvedic plant Safed Musali. Materials and methods: Comparative evaluations of Macro and microscopical, physico-chemical parameters of tubers of all five species were investigated and preliminary phytochemical analysis, estimation of major phytochemicals and TLC profiles were also carried out for qualitative phytochemical evaluation. In-vitro antioxidant and anticancer activity was carried out for extract of tubers of all five species. Results: Macro, micro, powder microscopical parameters of tubers of five species were examined and recorded the result. Tubers of all the five species are distinct in their morphology as well as anatomical characters. Physicochemical characters (Ash values, Loss on drying (LOD), swelling index and foaming index) as well as total saponin content shows great variability among five species. Results of In-vitro antioxidant by DPPH method shows difference in antioxidant potential between tubers of all five species. Extract of tubers of all five species do not show any type of In-vitro anticancer activity by SRB method against HL 60 leukemia cell line. Conclusion: All of the evaluated parameters are very good pharmacognostic standards for future comparative identification and authentication of specific species because all five species shows morphological, anatomical, chemical differences as well as varies in antioxidant potential.

}, keywords = {Arundinaceum, Borivilianum, Chlorophytum Comosum, DPPH, HL-60, Laxum, SRB, Tuberosum.}, doi = {10.5530/pj.2015.5.12}, author = {Sharada Laxman Deore and Neha Brijmohan Jajoo and Kailaspati Prabhakar Chittam and Tushar Atmaram Deshmukh} } @article {1474, title = {Rapid and high yield Extraction method for Saponins from Safed Musli}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {16th Jan, 2015}, pages = {210-214}, type = {Original Article}, chapter = {210}, abstract = {

Objectives: We aimed to develop, compare and optimise rapid and high yield extraction method for saponins of safed musli using conventional extraction techniques and as well as modern microwave assisted solvent extraction method. Materials and methods: Roots of Safed musli (Chlorophytum borivilianum) are extracted by maceration, soxhlet, sonication and microwave methods. Extract further fractionated to obtain total saponins. Microwave assisted solvent extraction (MASE) method is optimised using Taguchi L9 orthogonal array design. Total saponins are estimated by High Performance Thin Layer chromatography (HPTLC) from all extracts obtained by different methods. Results: Factors namely temperature, irradiation time, irradiation power and powder size which potentially affects extraction efficiency are considered while optimizing MASE by statistical orthogonal array design procedure and saponins are quantified using HPTLC. Under developed optimum conditions, MASE showed significantly higher yield (5.11\%) and drastic reduction in extraction time (4 min) than conventional extraction methods. Conclusion: saponins of safed musli shown highest yield in MASE and then maceration, soxhlet and sonication followed. The developed and optimised method of saponin extraction by MASE can have huge industrial applications after scale up.

Key words: HPTLC, Microwave assisted solvent extraction, Maceration Saponins, Orthogonal test L9 (34) Sonication, Taguchi Design.

}, keywords = {HPTLC, Maceration Saponins, Microwave assisted solvent extraction, Orthogonal test L9 (34) Sonication, Taguchi Design.}, author = {Sharada Laxman Deore and Bhushan Arun Baviskar and Ashwini Suresh Rangari} } @article {50, title = {Rapid and high yield Extraction method for Saponins from Safed musli}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Jul-Aug 2015}, pages = {210-214}, type = {Original Article}, chapter = {210}, abstract = {

Objectives: We aimed to develop, compare and optimise rapid and high yield extraction method for saponins of Safed musli using conventional extraction techniques and as well as modern microwave assisted solvent extraction method. Materials and methods: Roots of Safed musli (Chlorophytum borivilianum) are extracted by maceration, soxhlet, sonication and microwave methods. Extract further fractionated to obtain total saponins. Microwave assisted solvent extraction (MASE) method is optimised using Taguchi L9 orthogonal array design. Total saponins are estimated by High Performance Thin Layer chromatography (HPTLC) from all extracts obtained by different methods. Results: Factors namely temperature, irradiation time, irradiation power and powder size which potentially affects extraction efficiency are considered while optimizing MASE by statistical orthogonal array design procedure and saponins are quantified using HPTLC. Under developed optimum conditions, MASE showed significantly higher yield (5.11\%) and drastic reduction in extraction time (4 min) than conventional extraction methods. Conclusion: Saponins of Safed musli shown highest yield in MASE and then maceration, soxhlet and sonication followed. The developed and optimised method of saponin extraction by MASE can have huge industrial applications after scale up.

}, keywords = {HPTLC, Maceration Saponins, Microwave assisted solvent extraction, Orthogonal test L9 (34) Sonication, Taguchi Design}, doi = {10.5530/pj.2015.4.1}, author = {Sharada Laxman Deore and Bhushan Arun Baviskar and Ashwini Suresh Rangari} }