@article {1163, title = {Cytotoxicity of Selenium-Enriched Chinese Kale (Brassica oleracea var. alboglabra L.) Seedlings Against Caco-2, MCF-7 and HepG2 Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {674-681}, type = {Original Article}, chapter = {674}, abstract = {

Background: The Selenium-enriched Chinese kale (Brassica oleracea var. alboglabra L.) seedlings (Se-KS) have been known for its antioxidant activities, however its cytotoxic effects on various cancer cells are yet to be reported. Objective: The objective of this work was to study the cytotoxic effects of Se-KS on Caco-2, MCF-7 and HepG2 cancer cells. Materials and Methods: Freeze-dried seedlings were ground and incubated in 0.1 M citrate phosphate buffer pH 7.0 for 1 h at 37{\textdegree}C and extracted with dichloromethane to obtain total isothiocyanate (ITC) content which was quantified using the 1,2-benzenedithiole (BDT)-based cyclocondensation assay. The extracts from fresh seedlings were used to determine the cytotoxic effect on Caco- 2, MCF-7 and HepG2 cancer cells. Results: Se-KS was found to contain total ITC content at 1.02 mmol/100 g dry weight (DW) which was significantly lower than that of 7-day old broccoli microgreens (1.60 mmol/100 g DW) as reference Cruciferous vegetables. In addition, Se-KS extract exhibited cytotoxic effects in a dose- and time-dependent manners. The lowest IC50 value of 82.83 μg/mL at 72 h was derived from HepG2 cells and the highest IC50 value of 164.00 μg/mL at 72 h was from MCF-7 cells suggesting that the Se-KS extract was most effective against HepG2 cells. Cancer cells showed signs of apoptotic bodies over 72 h and DNA fragmentations at 24 h indicating that the Se-KS extract was able to induce apoptosis in cancer cells in addition to cytotoxic effect. Conclusion: Thus, Se-KS could be a novel source of organo selenium with chemopreventive benefits for functional food development.

}, keywords = {Caco-2, HepG2, Isothiocyanate, Kale, MCF-7, Selenium}, doi = {10.5530/pj.2020.12.99}, author = {Vijitra Luang-In and Worachot Saengha and Benjaporn Buranrat and Anut Chantiratikul and Nyuk Ling Ma} } @article {343, title = {Inhibition of Caco-2 and HeLa proliferation by Terminalia carpentariae C. T. White and Terminalia grandiflora Benth. extracts: Identification of triterpenoid components}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {May 2017}, pages = {441-451}, type = {Original Article}, chapter = {441}, abstract = {

Background: Terminalia spp. are characterised by their high antioxidant capacities and many have anticancer activity. This study examines the anti-proliferative activity of T. carpentariae leaf and T. grandiflora leaf, fruit and nut extracts against Caco-2 and HeLa carcinoma proliferation. Materials and Methods: Powdered T. carpentariae leaf and T. grandiflora leaf, fruit and nut were extracted and tested for anti-proliferative activity against Caco-2 and HeLa cancer cell lines using colorimetric cell proliferation assays. Toxicity was evaluated using an Artemia franciscana nauplii bioassay. The extract with the most potent anti-proliferative activity was examined using GCMS analysis and triterpenoid compounds were identified by comparison with a compound database. Results: T. carpentariae leaf and T. grandiflora leaf, fruit and nut extracts displayed potent anti-proliferative activity against Caco-2 and HeLa carcinoma cells. The methanolic T. grandiflora leaf extract was particularly effective at blocking the proliferation of the colorectal carcinoma Caco-2 (IC50 = 372 \μg/mL). The methanol T. carpentariae and T. grandiflora leaf extracts were similarly potent inhibitors of HeLa cervical cancer cell proliferation with IC50 values of 864 and 833 \μg/mL respectively. The methanolic T. grandiflora fruit and nut extracts, as well as all aqueous and ethyl acetate extracts, were moderate to good inhibitors of carcinoma proliferation. In contrast, chloroform and hexane extracts were generally devoid of anti-proliferative activity. The methanolic T. grandiflora extracts displayed low toxicity in the Artemia nauplii bioassay. All other extracts were non-toxic. GC-MS analysis of the methanolic T. grandiflora leaf extract identified 3 lanostane and 2 pentacyclic triterpenoids. Conclusion: The low toxicity and anti-proliferative activity observed with the T. carpentariae and T. grandiflora extracts against Caco-2 and HeLa indicate their potential for the prevention and treatment of some cancers.

}, keywords = {Anticancer activity, Australian plants, Caco-2, Chemotherapy, Combretaceae, HeLa, Native almond, Wild peach}, doi = {10.5530/pj.2017.4.74}, url = {/files/PJ-9-4/10.5530pj.2017.4.74}, author = {Reece Courtney and J. Sirdaarta and A. White and I. E. Cock} }