@article {216, title = {Revelation of β-sitosterol from Benincasa hispida Seeds, Carissa congesta Roots and Polyalthia longifolia Leaves by High Performance Liquid Chromatography}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {September 2016}, pages = {610-613}, type = {Original Article}, chapter = {610}, abstract = {

Background: Fruit juice of Benincasa hispida (BH) is regarded as Vrindamadhava that is recommended for internal use in snake\’s bites. The fruits have been reported to contain good amount of proteins, enzymes, Vitamin B1 and C1 flavonoid C-glycoside, terpenes, phenolic acids and free sugars such as glucose, rhamnose, mannitol, uronic acid, astilbin, catechin, naringenin, pectic polysaccharides and even some trace metals. Polyalthia longifolia (PL) has been well known for its ayurvedic preparations such as Sitaphala kula, Kaphapitashama, Anulomak and Krimighna. PL leaves has been reported to contain phytoconstituents like polylongine, Aporphine-Noxide alkaloids, allo-aromadendrene, caryophyllene oxide, \β-caryophyllene, \β-selinene, \α-humulene, \α-pinene and camphene. Carissa congesta (CC), known as Bengal currant, have yielded 2-acetyl phenol, carissone, carindone and Des-n-methylnoracronycine. \β-sitosterol, an important phytoconstituent recognized from all these selected plants has been well known for its ethnopharmacological importance. In this new study, the research team members has focused on determining the percentage of the \β-sitosterol present in the BH seeds, CC roots and PL leaves by subjecting the extract to High Performance Liquid Chromatography (HPLC). Materials and Methods: BH, CC and PL plants were shade-dried and extracted by suitable extraction methods. In HPLC, peaks obtained in the extracts were compared with the standard by matching their retention time. Results: The amounts of \β-sitosterol present in the BH seeds, CC roots and PL leaves extracts obtained at 254 nm were found to be 36.00, 7.46 and 3.21\% w/w respectively. Conclusion: Thus, BH, PL and CC extracts were said to contain \β-sitosterol as a key constituent.

}, keywords = {Benincasa hispida, Carissa congesta, HPLC, Polyalthia longifolia, β-sitosterol.}, doi = {10.5530/pj.2016.6.15}, author = {Gaurav Mahesh Doshi and Pratip Kashinath Chaskar and Hemant Devidas Une} } @article {1451, title = {Elucidation of β -sitosterol from Benincasa hispida Seeds, Carissa congesta Roots and Polyalthia longifolia Leaves by High Performance Thin Layer Chromatography}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {29th Apr, 2015}, pages = {221-227}, type = {Original Article}, chapter = {221}, abstract = {

Background: Fruits of Benincasa hispida (BH) is regarded as Valliphala due to its vast plethora of medicinal properties, Carissa congesta (CC) is an imperative local plant particularly in rural communities and Polyalthia longifolia (PL) is an ornamentally significant traditionally relevance plant in India system. \β-sitosterol, an active constituent identified from enormous plants has been reported to possess excellent amount of pharmacotherapeutic potential by number of researchers. Objective: In the recent studies, the research team focuses on determining the percentage of the \β-sitosterol present in the BH seeds, CC roots petroleum ether extracts as well as PL leaves ethanolic extract by chromatographic technique in harmony with High Performance Thin Layer Chromatography. Materials and Methods: Respective parts of BH, CC and PL plants were shade-dried and extracted by appropriate extraction methods followed by identification of \β-sitosterol from the extracts by High Performance Thin Layer Chromatography after preliminary phytochemical screening extracts for the constituents. Results: The amount of \β-sitosterol present in the BH seeds, CC roots and PL leaves extracts was found to be 23.00, 5.94 and 1.81 \% w/w respectively. Research studies elucidated a peak that coincided with standard peak of \β-sitosterol suggesting the presence of constituent in the extracts. Conclusion: Thus, extracts contains important constituent of \β-sitosterol in BH, CC and PL.

Key words:Benincasa hispida, Carissa congesta, HPTLC, Polyalthia longifolia, \β-sitosterol.

}, keywords = {Benincasa hispida, Carissa congesta, HPTLC, Polyalthia longifolia, β-sitosterol.}, author = {Gaurav Mahesh Doshi and Pratip Kashinath Chaskar and Hemant Devidas Une} } @article {54, title = {Elucidation of β-sitosterol from Benincasa hispida Seeds, Carissa congesta Roots and Polyalthia longifolia Leaves by High Performance Thin Layer Chromatography}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Jul-Aug 2015}, pages = {221-227}, type = {Original Article}, chapter = {221}, abstract = {

Background: Fruits of Benincasa hispida (BH) is regarded as Valliphala due to its vast plethora of medicinal properties, Carissa congesta (CC) is an imperative local plant particularly in rural communities and Polyalthia longifolia (PL) is an ornamentally significant traditionally relevance plant in India system. \β-sitosterol, an active constituent identified from enormous plants has been reported to possess excellent amount of pharmacotherapeutic potential by number of researchers. Objective: In the recent studies, the research team focuses on determining the percentage of the \β-sitosterol present in the BH seeds, CC roots petroleum ether extracts as well as PL leaves ethanolic extract by chromatographic technique in harmony with High Performance Thin Layer Chromatography. Materials and Methods: Respective parts of BH, CC and PL plants were shade-dried and extracted by appropriate extraction methods followed by identification of \β-sitosterol from the extracts by High Performance Thin Layer Chromatography after preliminary phytochemical screening extracts for the constituents. Results: The amount of \β-sitosterol present in the BH seeds, CC roots and PL leaves extracts was found to be 23.00, 5.94 and 1.81 \% w/w respectively. Research studies elucidated a peak that coincided with standard peak of \β-sitosterol suggesting the presence of constituent in the extracts. Conclusion: Thus, extracts contains important constituent of \β-sitosterol in BH, CC and PL.

}, keywords = {Benincasa hispida, Carissa congesta, HPTLC, Polyalthia longifolia, β-sitosterol}, doi = {10.5530/pj.2015.4.3}, author = {Gaurav Mahesh Doshi and Pratip Kashinath Chaskar and Hemant Devidas Une} }