@article {2143, title = {Cardioprotective Effects of Thymoquinone on Myocardial Fibrosis}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {924-927}, type = {Research Article}, chapter = {924}, abstract = {

Introduction: Thymoquinone (TQ) is one of the active ingredients in herbal plants such as Nigella sativa which has antioxidant and anti-inflammatory properties thus may inhibits cardiac fibrosis formation. This study aims to determine the effectiveness of Thymoquinone as a cardioprotective agent in suppressing the extent of fibrosis in Wistar rats induced with lipopolysaccharide (LPS). Methods: This post-test only control study used 30 Wistar rats which were divided into 5 groups: saline, LPS-induced cardiac fibrosis, LPS-induced cardiac fibrosis treated with TQ 10 mg/mL, LPS-induced cardiac fibrosis treated with TQ 20 mg/mL, and LPS-induced cardiac fibrosis treated with TQ 40 mg/mL. Serum IL-6, GSH, and cTnT levels were measured using ELISA, and Mason{\textquoteright}s trichrome staining was used to assess myocardial fibrosis. Results: The LPS10+TQ20 and LPS10+TQ40 groups exhibited significantly lower levels of IL-6 compared to the LPS10+TQ10 group (p \< 0.05). GSH levels did not show a significant decrease in the TQ groups across different doses (p=0.771). The TQ-treated group demonstrated lower cTnT levels compared to the LPS-only group (p\<0.05). Thymoquinone treatment resulted in reduced fibrosis area compared to the LPS10 group (p\<0.05). Conclusions: TQ has a promising cardioprotective effect on the formation of cardiac fibrosis in Wistar rats induced with LPS.

}, keywords = {Cardiac Fibrosis, Lipopolysaccharide, Nigella sativa, Oxidative stress.}, doi = {10.5530/pj.2023.15.176}, author = {Saugi Abduh and Purwanto Bambang and Dirgahayu Paramasari and Soetrisno} } @article {2144, title = {Effect of Lactobacillus Plantarum IS-10506 on Paneth Cell Regeneration in the Ileum of Sprague Dawley Rats}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {928-932}, type = {Research Article}, chapter = {928}, abstract = {

Background: Pathogenic Escherichia coli (E. coli) is the most common infectious agent among children in developing countries. Indigenous probiotics are not widely used to treat diarrhea and intestinal infections. This study aims to investigate the cell regeneration process of paneth cells after administration of Lactobacillus plantarum IS-10506 due to damage caused by Lipopolysaccharide (LPS) E. coli O55:B5, through the expression of MATH-1 and DEFA-6. Methods: This study used 64 paraffin blocks from Rattus norvegicus strain Sprague-Dawley divided into four groups. There were three treatments, KN, KL, KP and KPR groups, The KN group represent the administration of placebo. The KL group received LPS E. coli O55:B5 on day one. The KP group received LPS E. coli O55:B5 on the first day and Lactobacillus plantarum IS-10506 on the second day until six-day. The KPR group were administered Lactobacillus plantarum IS- 10506 six days prior to receiving LPS E. coli O55:B5, respectively. All groups, except KN, received LPS at a dose of 250 μg/kg body weight once, and Lactobacillus plantarum IS-10506 at a dose of 2.86x1010 CFU/ day. Evaluating paneth cell regeneration, DEFA-6, and MATH-1 expression immunohistochemistry was conducted on all tissues. Results: The expression of DEFA-6 and MATH-1 in the KP and KPR groups on day three of observation was significantly higher from the KL group. Even though the KL group achieved significant growth, the results of this expansion were significantly smaller than KP and KPR groups. Conclusion: After mucosal injury caused by LPS E. coli O55:B5, administration of probiotic Lactobacillus plantarum IS-10506 may increase paneth cell regeneration through differentiation and cell number.

}, keywords = {DEFA-6, Escherichia coli O55:B5, Lactobacillus plantarum IS-10506, Lipopolysaccharide, MATH-1., Paneth cells}, doi = {10.5530/pj.2023.15.177}, author = {Rasio Putra Hutama and Alpha Fardah Athiyyah and I.G.M. Reza Gunadi Ranuh and Andy Darma and Khadijah Rizky Sumitro and Wibi Riawan and Ingrid S. Surono and Subijanto Marto Sudarmo} } @article {433, title = {Hepatoprotective Effect of Curcumin and Capsaicin against Lipopolysaccharide Induced Liver Damage in Mice}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {September 2017}, pages = {947-951}, type = {Original Article}, chapter = {947}, abstract = {

Objective: The present study was undertaken to evaluate the possible ameliorative role of curcumin, capsaicin and their combination against lipopolysaccharide (LPS) induced hepatic toxicity in mice. Methods: Animals were distributed into five experimental groups: Normal control, vehicle control, curcumin, capsaicin and combined curcumin and capsaicin treatment groups respectively, for 7 days prior to LPS induced liver toxicity (3 mg/kg b.w. in saline). Hepatoprotective effect of individual and combined spice principles were evidenced by the measurement of serum marker enzyme activities such as, SGPT, ALP and TB and it was further confirmed by histopathological observation of liver tissue section. Results: The administration of LPS increased serum nonspecific enzymes (SGOT; 174.2\±3.79 IU/L, SGPT; 124.0\±3.14 IU/L, ALP; 320.15\±3.88 IU/L and total bilirubin level; 2.32\±1.23 mg/dL), however dietary curcumin and capsaicin decreased the activities of these non\–specific serum enzymes including total bilirubin indicating amelioration of the severe LPS induced hepatotoxicity, while the combined spice principles were more significant as shown by the levels of enzymes activities SGOT; 89.9\±1.39 IU/L, SGPT; 85.9\±1.83 IU/L, ALP; 138.4\±2.05 IU/L including total bilirubin level; 0.86\±0.03 mg/dL. Conclusion: Dietary curcumin and capsaicin individually are protective to LPS induced hepatotoxicity, the beneficial effect was found to be more when the two compounds were fed in combination.

}, keywords = {ALP., Capsaicin, Curcumin, Hepatoprotective activity, Lipopolysaccharide, SGOT, SGPT}, doi = {10.5530/pj.2017.6.148}, url = {http://fulltxt.org/article/201}, author = {Thriveni Vasanthkumar and Manjunatha Hanumanthappa and Prabhakar BT and Santhosh Kondajji Hanumanthappa} }