@article {1313, title = {Screening of Phytochemical Analysis and In vitro Bioactive of Polyherbal Formulation}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {November 2020}, pages = {1525-1533}, type = {Original Article}, chapter = {1525}, abstract = {

Introduction: Plants have the capability to synthesize various forms of phytochemical compounds as secondary metabolites. Method: In the present investigation phytochemicals such as alkaloids, tannins, glycosides, carbohydrates, reducing sugar, proteins, saponins, flavanoids, phenols, terpenoids and phytosterols were detected in polyherbal formulation A. marmelos, G. glabra and R. centrifolia. Results: The Total flavonoid, steroids, alkaloids and phenolic content was observed from fruit, root and pettles1.40mg QE/g DE, 12.14mg BE/g DE, 14.40mg AE/g DE and 99.33mg GAE/g DE. FTIR spectrum of the polyherbal sample revealed 5 major peaks at 2919.40 cm-1, 2357.62 cm-1, 1150.56 cm-1, 1076.22 cm-1 and 1015.64 cm-1. The antibacterial activity was maximum zone of inhibition (19 mm) was recorded in S. aureus strain and minimum zone of inhibition (5mm) was observed in S.mutans strain. The antioxidant study maximum and minimum scavenging DPPH, NOR, H2O2 and SOD activities (\%) of 62.28, 53.68, 39.67 \& 43.98 at 5 mg/ml and 39.88, 36.49, 5.9 and 34.94 at 1mg/ml was recorded. The polyherbal sample exhibited significant albumin denaturation, proteinase inhibitory, membrane stabilization and lipid peroxidation inhibitory activities as the maximum inhibition of 46.53\%, 36.7\%, 51.9\% and 64.71\% was observed at 500 μg/ml. Conclusion: Hence the present studies indicate good antibacterial, antioxidant and anti-inflammatory activities from the medicinal plants, A. marmelos, G. glabra and R. centrefolia proves the possibility of its utilization as an additional potent source medicinal uses.

}, keywords = {Antimicrobial activity, antioxidant activity, FTIR, HR-LCMS, Phyto chemical, Secondary metabolites}, doi = {10.5530/pj.2020.12.209}, author = {Vasanth MP and KG Purushotham} } @article {434, title = {Phytochemical Screening, GC-MS, FT-IR Analysis of Methanolic Extract of Curcuma caesia Roxb (Black Turmeric)}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {September 2017}, pages = {952-956}, type = {Original Article}, chapter = {952}, abstract = {

Aims: The purpose of the current study is to monitor the phytochemical constituents in the Curcuma caesia Roxb.by GC MS and FT-IR analysis. Methods: The Rhizomes of Curcuma caesia Roxb was extracted with Methanol at room temperature for 8 h. The bioactive compounds of Curcuma caesia Roxb have been evaluated using GC-MS and FT-IR. Results: Preliminary phytochemical analysis revealed the presence of tannins, terpenoids, flavonoid, alkaloid, phenol, phytosterol Quinones and saponins. Totally 15 compounds were identified and the chromatograph showed peaks with individual compounds. The major constituents were identified in the Methanolic extract were \α-Santalol (46.90\%), Retinal (10.72\%), Ar-tumerone(10.38\%), Alloaromadendrene (5.93\%), Megastigma-3,7(E),9-triene (4.80\%), Benzene, 1-(1,5-dimethyl- 4-hexenyl)-4-methyl(4.38\%) , 5,8,11,14,17-Eicosapentaenoic acid, methyl ester, (all-Z)-(4.26\%) Tricyclo[8.6.0.0(2,9)]hexadeca-3,15-diene, trans-2,9-anti-9,10-trans-1,10 (3.26\%) and many other compounds were identified as low level. The FTIR analysis confirmed the presence of N-H , O-H , C=C , C-H, C-O and CH3 functional groups. Conclusion: The result of this study offer a platform of using Curcuma caesia Roxb as herbal alternative for various diseases and it can be used as functional and pharmaceutical food.

}, keywords = {Curcuma caesia Roxb, FT-IR, GC MS, Phyto chemical, α-Santalol and Retinal.}, doi = {10.5530/pj.2017.6.149}, url = {http://fulltxt.org/article/202}, author = {Muthukumaran Pakkirisamy and Suresh Kumar Kalakandan and Karthikeyen Ravichandran} }