@article {1616, title = {Comparison of Colchicine Content in Gloriosa superba L., Root after Pretreatment by Thai Traditional Medicine Methods}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {July 2021}, pages = {942-948}, type = {Research Article}, chapter = {942}, abstract = {

Introduction: In Thai traditional medical practice, the roots of Gloriosa superba L. must be treated prior to use as Thai herbal remedies. Colchicine is a major active chemical constituent in the roots. It was reported that lethal doses of colchicine were 7-26 mg. There have been two pieces of evidence that indicated 5 different pretreatment methods of Gloriosa superba L., roots. Therefore, this research aimed to compare the amount of colchicine before and after pre-treated by 5 different methods and compare colchicine content after pretreatment between methods. Methods: Provide five different locations of fresh Gloriosa superba L., roots. Each sample divided into 6 groups. The first group would be treated as a control, the second to the sixth group was pre-treated by roasting, burning, boiling, stewing, and grilling which follow the methods from two pieces of evidence. Subsequently, quantitative analysis by High Performance Liquid Chromatography. Results: The amount of colchicine in roots before and after pre-treated by roasting, burning, boiling, stewing, and grilling methods were significantly reduced by 45.61{\textpm}10.61\% (p=0.000), 37.35{\textpm}14.15\% (p=0.000), 44.55{\textpm}8.60\% (p=0.000), 76.40{\textpm}10.70\% (p=0.000), and 30.35{\textpm}11.31\% (p=0.000), respectively. The stewing method was the greatest decrease amount of colchicine which significantly different from other methods. (p=0.000). Conclusion: All 5 methods were significantly reduced amounts of colchicine in the roots of Gloriosa superba L. They were effective methods to pre-treat the roots of Gloriosa superba L. prior to use as Thai herbal remedies.

}, keywords = {Colchicine, Gloriosa superba L., HPLC, Pretreatment methods, Thai traditional medicine}, doi = {10.5530/pj.2021.13.121}, author = {Sasithorn Tandhavadhana} } @article {848, title = {Reduction of Colchicine Content from Radix Gloriosae Superbae Preparata}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {February 2019}, pages = {310-314}, type = {Original Article}, chapter = {310}, abstract = {

Introduction: Gloriosae Superbae Radix is a crude drug employed in Thai herbal remedies for several ailments. Colchicine is known as an active constituent in the roots. It was reported that 7-11 mg. of colchicine may causes lethal effect in human. In Thai traditional medical practice, the roots must be treated prior use to prepare Thai herbal preparations. However, pre-treated method has not yet been well established in any literature. This study aimed to document the pre-treated method and to quantitatively compare the amount of colchicine both before and after pre-treated. Methods: Firstly, interviewing with Thai traditional medicine experts and document the pre-treated methods of Gloriosae Superbae Radix to conclude the 2 commonly used methods. Two pre-treated methods (roasting and burning) were chosen for further study. Colchicine in Gloriosae Superbae Radix from 8 sources were analyzed before and after pre-treat by High Performance Liquid Chromatography (HPLC). Results: After pre-treat by roasting and burning, amount of colchicine in root were significantly decreased by 40.61{\textpm}9.55\% (p=0.000) and 26.79{\textpm}10.89\% (p=0.001), respectively. Comparison of colchicine contents of samples after roasting and burning, the amount of colchicine decreased non statistically insignificantly (p=0.110). Conclusion: Pre-treats of Gloriosae Superbae Radix by roasting and burning had significantly reduce colchicine content. Both methods have been proven to be the effective ways in preparing certain potentially toxic crude drugs before using in compounding into Thai herbal remedies.

}, keywords = {Colchicine, Gloriosae Superbae Radix, HPLC, Pre-treat method, Thai herbal remedy}, doi = {10.5530/pj.2019.11.46}, author = {Sasithorn Tandhavadhana and Chayan Picheansoonthon} } @article {450, title = {Evaluation of Anti Arthritic Potential of Gloriosa superba (L.) Elite Germplasm Collected from Eastern Himalayas, India}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {November 2017}, pages = {s87-s92}, type = {Original Article}, chapter = {s87}, abstract = {

Introduction: Gloriosa superba (L.) is a traditionally known medicinal plant for its potential antigout property. The species is rich source of colchicine alkaloid and is commercially exploit in the international market for the same. Method: In the present study, elite chemotype of G. superba was identified from natural population in Eastern Himalayas based on their colchicine content through HPTLC calibrated method. The selected elite chemotypes were further evaluated for in vitro anti-arthritic potential via inhibition of protein denaturation along with hydroxyl radical scavenging potential. Result: The HPTLC quantification data reveals that the content of colchicine varies from 0.044 to 0.184\% having maximum content in NBG-128 from Jorhat, Assam. The results of bioassay reflect a potentiating anti-arthritic and hydroxyl radical scavenging with statistically insignificant difference within the elite germplasms. Conclusion: The presence of bioactive polyphenolics with significant hydroxyl radical scavenging will further suggest that inhibition of inflammatory mediator cells by extract is superimposed action of colchicine and other chemical inhibitors like polyphenolics. The study will aid in site specific exploration of high metabolite yielding chemotype(s) with validated pharmacological action for commercial cultivation to meet out the industrial demand of colchicine and herbal product development.

}, keywords = {Anti arthritic, Colchicine, Elite chemotype, G. superba, HPTLC}, doi = {10.5530/pj.2017.6s.162}, url = {http://fulltxt.org/article/387}, author = {Ankita Misra and Akanksha Srivastava and Mohammad Khalid and Poonam Kushwaha and Sharad Srivastava} }