@article {1629, title = {Anti-Cancer Potential of Nggorang Leaves Extract (Salvia Occidentalis SW.) as a Protein P53 Supressor in T47D Cells}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {July 2021}, pages = {1036-1045}, type = {Research Article}, chapter = {1036}, abstract = {

Breast cancer is one of the most common types of cancer in women. The high incidence of breast cancer has led to the development of anticancer drugs that are more selective against cancer cells without damaging normal tissues. One of the alternatives in cancer treatment by looking for natural sources that can be developed, Nggorang leaves (Salvia occidentalis Sw.). This plant is found in Tenda Village, Langke Rembong District, Manggarai Regency, NTT Province, when the leaves are harvested for one year and are used as medicine. As a preventive, this leaf has been used for generations as an anticancer (7 leaves boiled with 200 ml of water to 100 ml and then drunk), for wounded breast cancer (crushed leaves and attached to the wound); stamina enhancer, cough, influenza, hemorrhoids, diarrhea, nosebleeds (Primary data, 2014). This study aims to prove the potential of EDG (Nggorang Leaves Extract) as an antiproliferative against Hela cancer cells and protein P53 suppressor. The method used is the Quasy experiment, because this study uses laboratory tests in sample testing. The results of the cytotoxic test of Nggorang Leaves Extract have the potential to be anti-proliferative against cancer cells T47D IC50 at 201 ppm and Nggorang Leaves Extract (EDG) has the potential to increase p53 gene suppression in T47D cancer cells by 94.13\% at a concentration of 50 ppm.

}, keywords = {Anticancer, Nggorang Leaves Extract (Salvia occidentalis Sw), Protein P53, T47D}, doi = {10.5530/pj.2021.13.134}, author = {Sisilia Teresia Rosmala Dewi and M Sabir and Sesilia Rante Pakadang and Sainal Edi Kamal and Santi Sinala} } @article {1370, title = {Cytotoxicity Study of Ethanol Extract of Bintangor Leaf (Calophyllum soulattri Burm.f) on T47D Breast Cancer Cell Line (Cytotoxicity Study with MTT Assay Method)}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {362-367}, type = {Original Article}, chapter = {362}, abstract = {

Introduction: The public has used Bintangor leaf (Calophyllum soulattri Burm.f) for various medical treatments, including treated inflamed eyes and gout. Aim: This research aimed to determine the cytotoxic effect of ethanol extract and fraction of Calophyllum soulattri Burm. f leaf toward T47D breast cancer cell. Methods: The test used T47D breast cancer cells, the 3-4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium bromide (MTT) test method, and ELISA Reader to determine the absorbance. This method{\textquoteright}s principle was the presence of tetrazolium salts by the reductase system in the mitochondria of living cells formed purple formazan crystals. The used parameter was the value of IC50. Results: The result showed that ethanol extract, n-hexane fraction, ethyl acetate fraction, and butanol fraction did not have a cytotoxic effect on T47D breast cancer cell. The values of IC50 respectively are 585.31 μg/ml; 409.33 μg/ ml; 534.08 μg/ml; and 563.22 μg/ml. Conclusion: Ethanol extract and Calophyllum soulattri Burm.f leaf fraction did not have a cytotoxic effect on T47D breast cancer cells.

}, keywords = {Bintangor Leaf, Breast Cancer Line, Calophyllum soulattri Burm.f, Cytotoxicity, MTT Assay, T47D}, doi = {10.5530/pj.2021.13.46}, author = {Elidahanum Husni and Fatma Sri Wahyuni and Hanifa Nurul Fitri and Elsa Badriyya} } @article {466, title = {Cytotoxic Activities of Fractions from Dioscorea bulbifera L. Chloroform and Methanol Extracts on T47D Breast Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {December 2017}, pages = {33-38}, type = {Original Article}, chapter = {33}, abstract = {

Objective: To elucidate cytotoxic activity of fractions from chloroform and methanol extracts of D. bulbifera organs on T47D breast cancer cells. Method: The vegetative organs of D. bulbifera were extracted gradually using chloroform and methanol. Cytotoxicity tested on T47D cells using MTT Assay. The most toxic extract was fractioned by vacuum liquid chromatography (VLC) followed by thin layer chromatography (TLC). The extract and fractions potential were tested on the Vero cells using the same method as cancer cells. The most toxic fraction was analyzed using TLC followed by the application of various spray reagents for the identification of active compound. Results: The chloroform extract of the D. bulbifera leaves was the highest cytotoxic on T47D cells (IC50 115.63\±86.01 \μg/mL). Moreover, the cytotoxicity test on the combined fractions of leaves chloroform extract showed that fraction 5 (F5) and fraction 6 (F6) were the most toxic fractions compared to those of other fractions. The IC50 of both fractions were 14.55\±8.62 and 7.12\±4.43 \μg/mL respectively. However, Its were very weak compared to those of cancer medicine (Doxorubicin) with the IC50 was 0.04\±0.02 \μg/mL. Potential fractions were not toxic against Vero cells with IS\>10. The active compounds in those fractions were alkaloid and terpenoid. Conclusion: Chloroform extract of the D. bulbifera leaves had the highest cytotoxic effect on T47D cells. Potential fractions were not toxic against Vero cells. The active compounds in those fractions were alkaloid and terpenoid.

}, keywords = {Cytotoxicity, D. bulbifera, MTT Assay, Secondary metabolites, T47D}, doi = {10.5530/pj.2018.1.7}, url = {http://fulltxt.org/article/362}, author = {Rinto Muhammad Nur and Laurentius Hartanto Nugroho} } @article {730, title = {Cytotoxic Activity of Ethanol Extract of Arbuscular Mycorrhizal Fungi Induced Ginger Rhizome on T47D Breast Cancer Cell Lines}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1133-1136}, type = {Original Article}, chapter = {1133}, abstract = {

Objective: A study of investigate the cytotoxicity activity of ethanolic extract of ginger (Zingiber officinale Rosc.) induced with arbuscular mycorrhizal fungi (AMF) against T47D cells line breast cancer have been conducted. Methods: Cytotoxicity were determined using the \“microtetrazolium (MTT) Assay\”, by measuring the activity of mitochondrial dehydrogenase in living cells that have ability to convert pale yellow of dissolved MTT to purple formazan product. The extract used at various concentration (0.1, 1.0, 10 and 100 \μg / mL. The level of cytotoxic actifity was determined by calculating the inhibitory concentration (IC50) value that was based on the precentage of cell death after 24 h treatment with the extract. The change of cell morphology were observed by using inverted microscope. Results: The statistic results proved that ethanol extract of AMF induced ginger rhizome could barriers T47D breast cancers significantly at concentrations of 10 \μg / mL and 100 ug / mL, with IC50 value was 12.5 \± 3.73 \μg / mL. centration of 0.1 \μg / mL, 1.0 \μg / mL, 10 \μg / mL and 100 mg / mL. Results of statistical analysis showed that the ethanol extract of ginger rhizome induced AMF at a concentration of 10 \μg / mL and 100 \μg / mL was able to inhibit the growth of breast cancer cells T47D significantly. Conclusion: The results showed the ethanol extract of AMF induced ginger rhizome was potential as herbal medicine for cancer-related ailments with IC50 value was 12.5 \± 3.73 \μg / mL.

}, keywords = {AMF, Breast cancer, Cytotoxicity, Ginger, MTT Assay, T47D}, doi = {xx10.5530/pj.2018.6.193}, author = {Netty Suharty and Fatma Sri Wahyuni and Dachriyanus} }