@article {555, title = {Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {x}, pages = {XX-XX}, type = {x}, chapter = {XX}, abstract = {

Objective: Gynura procumbens (Lour.) Merr is a well-known traditional herb and is widely used for traditional medicine by human. Previous clinical studies have shown the benefit effect of this leaves with inflammation, high blood pressure and others. This study aims to investigate the effect of methanol, ethanol, and ethyl acetate extracts of Gynura procumbens on U-87 cell line, human glioblastoma multiforme cell line. Methods: In the present study, effect of methanol, ethanol and ethyl acetate extracts on U-87 cell line was determined by MTT-based anti-proliferative assay and any significant changes to the cells towards apoptotic changes was observed under a light microscope. Results: The ethanol and methanol extracts of Gynura procumbens was found to have high anti-proliferative effect on U-87 cell line with IC50 less than 20\μg/ml. By comparing IC50 value, ethanol and methanol extracts of Gynura procumbens had high potential for anti-proliferative effect on U- 87 cell line. Therefore, ethanol and methanol extracts can be a potential anti-proliferative effect on U-87 cell line. Conclusion: To the best of our knowledge, this is the first study of exploring the effect of Gynura procumbens extracts using three different polarity (methanol, ethanol, ethyl acetate) effect on U-87 cell line, Human glioblastoma multiforme cells.

Key words: Gynura procumbens, Ethanol, Methanol, U87 cell line, Antiproliferative.

}, keywords = {Antiproliferative., Ethanol, Gynura procumbens, Methanol, U87 cell line}, doi = {10.5530/jppcm.2018.2s.xx}, author = {Mohd Adzim Khalili Rohin and Mimie Noratiqah Jumli and Norhaslinda Ridzwan and Atif Amin Baig and Ahmad Zubaidi Abdul Latif and Norhayati Abd Hadi} } @article {586, title = {Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {492-496}, type = {Original Article}, chapter = {492}, abstract = {

Objective: Gynura procumbens (Lour.) Merr is a well-known traditional herb and is widely used for traditional medicine by human. Previous clinical studies have shown the benefit effect of this leaves with inflammation, high blood pressure and others. This study aims to investigate the effect of methanol, ethanol, and ethyl acetate extracts of Gynura procumbens on U-87 cell line, human Glioblastoma multiforme cell line. Methods: In the present study, effect of methanol, ethanol and ethyl acetate extracts on U-87 cell line was determined by MTT-based anti-proliferative assay and any significant changes to the cells towards apoptotic changes was observed under a light microscope. Results: The ethanol and methanol extracts of Gynura procumbens was found to have high anti-proliferative effect on U-87 cell line with IC50 less than 20\μg/ml. By comparing IC50 value, ethanol and methanol extracts of Gynura procumbens had high potential for anti-proliferative effect on U- 87 cell line. Therefore, ethanol and methanol extracts can be a potential anti-proliferative effect on U-87 cell line. Conclusion: To the best of our knowledge, this is the first study of exploring the effect of Gynura procumbens extracts using three different polarity (methanol, ethanol, ethyl acetate) effect on U-87 cell line, Human Glioblastoma multiforme cells.

}, keywords = {Antiproliferative, Ethanol, Gynura procumbens, Methanol, U87 cell line}, doi = {10.5530/pj.2018.3.81}, url = {http://fulltxt.org/article/514}, author = {Mohd Adzim Khalili Rohin and Mimie Noratiqah Jumli and Norhaslinda Ridzwan and Atif Amin Baig and Ahmad Zubaidi Abdul Latif and Norhayati Abd Hadi} } @article {746, title = {In vitro Cytotoxicity Study on U87 Cells Using Root Extracts of Plumbago Species and GC-MS Study}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {November 2018}, pages = {s71-s76}, type = {Original Article}, chapter = {s71}, abstract = {

Plumbago is a genus of medicinal plants that are used in recent years to induce significant levels of apoptosis in various cancer cells. Purpose: The test substances that are obtained from Plumbago species are studied for their cytotoxicity against U87 cell line in a dose dependent manner and were first subjected to GC-MS study to know the bioactive constituents present in them. Methods: GC-MS was done using ethanol extracts of the roots of both the species. In vitro cytotoxicity of the roots of two species of Plumbago \–P. zeylanica (sample I) and P.\ auriculata (sample II) were tested against U87 cell line. Test samples were taken at concentrations ranging from 400\μg/ml to 3.12\μg/ml to determine the percentage growth inhibition of both the test substances on U87 cell line. Results: GC-MS analysis on root extracts of P.zeylanica showed the presence of 27 phytochemical constituents and P.auriculata 16 in number .The test substances, Sample I and Sample II exhibited a cytotoxic CTC50 value of 88.07\±4.4 and 23.11\±0.9 respectively. Conclusion: P.\ zeylanica is more effective than P.\ auriculata in terms of its cytotoxicity as well in the number of useful bioactive compounds.

}, keywords = {Bioactive constituents, Cytotoxicity, GC-MS, U87 cell line}, doi = {10.5530/pj.2018.6s.14}, author = {Sandhya Panicker and Veluthat Kolangara Haridasan} }