@article {858, title = {Evaluation of Anthelmintic Potential of Leaves and Fruits of Zanthoxylum rhetsa}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {May 2019}, pages = {475-478}, type = {Original Article}, chapter = {475}, abstract = {

Background: Zanthoxylum rhetsa of family Rutaceae is traditionally employed for treatment of intestinal worms, urinary tract infection, tooth ache, asthma, bronchitis and rheumatism. Thus, the objective of the study was to screen in vitro anthelmintic activity of leaves and fruits of Zanthoxylum rhetsa on Eisenia fetida and Tubifex tubifex. Material and Methods: Total methanol extract and solvent fractions of methanol extract were prepared by maceration and solvent solvent extraction process respectively. The chromatographic fingerprints of total methanol extracts of leaves and fruits were developed using HPTLC. Results: The leaf and fruit extracts showed the presence of alkaloids, flavonoids, terpenoids, coumarins, essential oils and saponins. The extracts exhibited significant anthelmintic activity as evidenced by decrease in paralysis death time in the treatment groups when compared to standard. Conclusion: The results suggest that fruits and leaves of Z rhetsa have promising anthelmintic activity and further studies are required to identify the phytochemicals responsible for anthelmintic activity.

}, keywords = {Anthelmintic, Fruit, HPTLC, Leaf, Phytochemicals, Zanthoxylum rhetsa}, doi = {10.5530/pj.2019.11.75}, author = {Rashmi Mallya and Fehmina Malim and Akanksha Naik and Milind Bhitre} } @article {679, title = {Comparison between Volatile Oil from Fresh and Dried Fruits of Zanthoxylum rhetsa (Roxb.) DC. and Cytotoxicity Activity Evaluation}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {827-832}, type = {Original Article}, chapter = {827}, abstract = {

Introduction: Zanthoxylum rhetsa is a tree found in northern Thailand. It has been used as a traditional herb with medicinal properties. However, the comparison of composition in volatile oil from fresh and dried fruit is still not fully investigated. In study, we evaluated the constituents in oil from fruits of Z. rhetsa and evaluated the cytotoxicity in non-small lung cancer cells. Methods: The hydro distillation was applied to afford the oil. The obtained oil was analyzed by GC-MS and evaluated the cytotoxicity and apoptosis in non-small lung cells by using MTT method and flow cytometry respectively. Results: Fresh and dried fruits provided a higher yield of volatile oil by approximately 10\% and 20\% respectively, compared to other studies. Twenty-eight compounds were identified and the major components of fresh and dried fruits were not distinctly different. The major component, \α-limonene, was found in dried fruits from Phayao, southern Nan and Chiang Rai province. A high content of \β-phellandrene was found in dried fruits from northern Nan province and the (+)-sabinene was found in high content of fresh fruits from southern Nan, Phayao and Chaing Rai province. Furthermore, the cytotoxicity tests displayed that all of oil products were active against lung cancer cells. Among the components investigated, fresh and dried fruits from southern Nan province showed to be the most potent (EC50 = 1.91 \± 0.53, 1.79 \± 0.43 \μL/mL, respectively). Conclusion: Volatile oil of Z. rhetsa exhibited a strong cytotoxic properties against cytotoxic may be potentially used as natural anticancer agents.

}, keywords = {Chemical composition, Cytotoxic activity, Local variation, Volatile oil, Zanthoxylum rhetsa}, doi = {10.5530/pj.2018.5.141}, author = {Sewan Theeramunkong and Maleeruk Utsintong} }