@article {1116, title = {Phytochemical Profile, Antioxidant Activity and Cell Line Study of Marine Red Macroalgae Eucheuma cottonii on Lung A-549 Cancer Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {276-281}, type = {Original Article}, chapter = {276}, abstract = {

Introduction: In this century, cancer has increased in incidence and become one of the deadliest disease in the world. However, to date, lung cancer treatments are still not fully effective, quite expensive and very exhaustive for the patient. Eucheuma cottonii is an abundant marine red macroalgae in Indonesia which have a potential anti-lung cancer properties. Aim of this research is to determine phytochemical profile of Eucheuma cottonii extracts, as well as to evaluate its antioxidant and cytotoxic effects on Lung A-549 cancer cells. Methods: Eucheuma cottonii obtained from Sorong beach, West Papua Province, Indonesia, were extracted with three different solvents, that is ethanol, ethylacetate, and n-hexane. These three Eucheuma cottonii extracts were identified for its phytochemical profiles, antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, and cytotoxic activity on lung A-549 cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Results: Phytochemical analysis revealed that Eucheuma cottonii contains metabolites of triterpenoid and alkaloid. Antioxidant activity evaluation showed ethanol extract of Eucheuma cottonii has IC50 value of 559.76 μg/mL against DPPH free radical. Whereas cytotoxicity evaluation showed that ethanol extract and ethylacetate extract of Eucheuma cottonii have cytotoxic effects on Lung A-549 cancer cells, with IC50 value of 251.73 μg/ mL and 261.41 μg/mL, respectively. Conclusion: These results suggesting that Eucheuma cottonii extract could be further developed as a natural anti-lung cancer agent.

}, keywords = {Antioxidant, Cell line study, Eucheuma cottonii, Lung A-549 cells, Phytochemical}, doi = {10.5530/pj.2020.12.43}, author = {Ade Arsianti and Gerry Kurniawan and Nadzila Anindya Tejaputri and Fona Qorina and Qotrunnada Fithrotunnisa and Norma Nur Azizah and Ajeng Megawati Fajrin} } @article {707, title = {Phytochemical Test and Cytotoxic Activity of Macroalgae Eucheuma cottonii against Cervical HeLa Cells}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1012-1017}, type = {Original Article}, chapter = {1012}, abstract = {

Intoduction: Marine resource of macroalgae Eucheuma cottonii from East Lombok, Nusa Tenggara Barat, Indonesia, have potential as anti-cervical cancer agent. Objectives: Finding cytotoxic activity of Eucheuma cottonii hexane, ethylacetate, chloroform and ethanol extracts against cervical HeLa cells by MTT cell proliferation assay. Methods: The extracts was tested in phytochemical and cytotoxic activity test. Phytochemical test to identify composition of secondary metabolite such as flavonoid, alkaloid, saponins, tannin, triterpenoid, steroid and glycoside. The amount of substances contained in the extract sample was analyzed by Thin Layer Chromatography (TLC). Cytotoxic activity using HeLa cells. Results: Phytochemical test of E. cottonii extracts showed the positive result for metabolite of flavonoid, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, n-hexane, chloroform, and ethyl acetate extracts of E. cottonii exhibited a strong cytotoxic activity against cervical HeLa cells with IC50 of 7.54 \μg/mL, 5.73 \μg /mL, 4.82 \μg /mL and 4.34 \μg / mL, respectively. Conclusion: The results suggest that macroalgae Eucheuma cottonii could be used as a new anti-cervical cancer\’s candidate.

}, keywords = {Anti-cervical cancer, Cytotoxicity, Eucheuma cottonii, HeLa cell lines, Phytochemical test, Seaweed}, doi = {10.5530/pj.2018.5.172}, author = {Ade Arsianti and Yully Astika Nugrahayning Aziza and Kartika Dwi Kurniasari and Baiq Kirana Dyahningrum Mandasari and Riathul Masita and Futihati Ruhama Zulfa and Micheylla Kusumaning Dewi and Cut Raisya Zahira Zagloel and Norma Nur Azizah and Rista Putrianingsih} }