@article {2038, title = {Metabolite Profiling of Compounds from Sargassum polycystum using UPLC-QToF-MS/MS}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {June 2023}, pages = {321-333}, type = {Research Article}, chapter = {321}, abstract = {

Background: There are many types of seaweed that have high economic value. Brown seaweed (Sargassum polycystum) can be used as a raw material in the industry and as a medicinal plant. Maintaining the quality of a compound requires an analytical method that can identify the diversity of metabolome profiles. Objective: This investigation seeks to discover the metabolite profile of S. polycystum from Sumenep, Madura Island, Indonesia, utilizing the UPLC-QToF MS/MS equipment. Materials and Methods: The extract was further fractioned using n-hexane, ethyl acetate, and water. The metabolite profiling of extract and fractions used the UPLC-QToF-MS/MS instrument. It was produced with SPE and then introduced into the MS Xevo G2-S QToF detector of the ACQUITY UPLC{\textregistered} H-Class System. The findings of the UPLC-QToF-MS/MS analysis were processed with the MassLynx 4.1 software to obtain chromatogram data and m/z spectra of each observed peak, which were then validated using the ChemSpider and MassBank databases. Results: Based on the results of metabolite profiling using UPLC-QToF-MS/MS, the 96 \% ethanol extract of S. polycystum indicated a total of 61 compounds, the n-hexane fraction indicated a total of 55 compounds, the ethyl acetate fraction indicated a total of 67 compounds, and the water fraction indicated a total of 49 compounds. Conclusion: There are 232 compounds in the extract and a fraction of S. polycystum consisting of 168 known compounds and 64 unknown compounds.

}, keywords = {Metabolite profiling, Sargassum polycystum, Seaweed, UPLC-QToF-MS/MS}, doi = {10.5530/pj.2023.15.82}, author = {Pramudita Riwanti and Intan Kris Prasetyanti and Burhan Ma{\textquoteright}arif} } @article {707, title = {Phytochemical Test and Cytotoxic Activity of Macroalgae Eucheuma cottonii against Cervical HeLa Cells}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1012-1017}, type = {Original Article}, chapter = {1012}, abstract = {

Intoduction: Marine resource of macroalgae Eucheuma cottonii from East Lombok, Nusa Tenggara Barat, Indonesia, have potential as anti-cervical cancer agent. Objectives: Finding cytotoxic activity of Eucheuma cottonii hexane, ethylacetate, chloroform and ethanol extracts against cervical HeLa cells by MTT cell proliferation assay. Methods: The extracts was tested in phytochemical and cytotoxic activity test. Phytochemical test to identify composition of secondary metabolite such as flavonoid, alkaloid, saponins, tannin, triterpenoid, steroid and glycoside. The amount of substances contained in the extract sample was analyzed by Thin Layer Chromatography (TLC). Cytotoxic activity using HeLa cells. Results: Phytochemical test of E. cottonii extracts showed the positive result for metabolite of flavonoid, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, n-hexane, chloroform, and ethyl acetate extracts of E. cottonii exhibited a strong cytotoxic activity against cervical HeLa cells with IC50 of 7.54 \μg/mL, 5.73 \μg /mL, 4.82 \μg /mL and 4.34 \μg / mL, respectively. Conclusion: The results suggest that macroalgae Eucheuma cottonii could be used as a new anti-cervical cancer\’s candidate.

}, keywords = {Anti-cervical cancer, Cytotoxicity, Eucheuma cottonii, HeLa cell lines, Phytochemical test, Seaweed}, doi = {10.5530/pj.2018.5.172}, author = {Ade Arsianti and Yully Astika Nugrahayning Aziza and Kartika Dwi Kurniasari and Baiq Kirana Dyahningrum Mandasari and Riathul Masita and Futihati Ruhama Zulfa and Micheylla Kusumaning Dewi and Cut Raisya Zahira Zagloel and Norma Nur Azizah and Rista Putrianingsih} }