@article {2294, title = {The Effect of Cryopreservation on the Sperm Ultrastructure of Mus Musculus Albinus Strain DDY: Comparison of Nakagata vs Modified vs Kitazato Cryoprotectants}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {June 2024}, pages = {563-569}, type = {Original Article}, chapter = {563}, abstract = {

Introduction: Sperm morphology analysis is very necessary to understand male fertility and the etiology of infertility. Currently, scanning electron microscopy (SEM) has been widely used to determine surface topology. In this study, we will compare the effects of spermatozoa cryopreservation using three different types of cryoprotectants, namely Nakagata, modification and Kitazato. The cryoprotectant compositions used are Nakagata (raffinose and skim milk), modified (glycerol and raffinose) and Kitazato (glycerol and trehalose). Methods: SEM analysis was carried out on 8 sperm samples before cryopreservation and after the freeze-thaw process. Results: The results obtain showed that cryoprotectant modification was able to protect spermatozoa morphology better than Nakagata and Kitazato. Analysis revealed damage to plasma membrane, acrosome and loss of mitochondria in all treatment groups compared to fresh sperm. SEM showed obvious signs of post-thaw damage such as missing plasma membranes, sperm showing damaged acrosomes and mitochondria in the middle showing structural disorganization. Conclusion: SEM revealed that cryopreservation caused ultrastructural damage to mice sperm due to freezing and thawing. These details provide valuable data for further research to minimize the damage caused by cryopreservation to mice sperm. Apart from that, further examination using TEM is recommended to obtain a more comprehensive picture.

}, keywords = {cryopreservation, glycerol, mice sperm, raffinose, Scanning electron microscope, Trehalose}, doi = {10.5530/pj.2024.16.88}, author = {Manggiasih Dwiayu Larasati and Silvia W. Lestari and Mulyoto Pangestu and Andon Hestiantoro and Kusmardi Kusmardi} } @article {741, title = {Chemical Composition and Ethnobotany of Livistona jenkinsiana Griff: An Endangered Thatching Palm Tree of Eastern Himalayas}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1202-1207}, type = {Original Article}, chapter = {1202}, abstract = {

Objectives: To study the uses of various parts and cultivation of Livistona jenkinsiana among tribal people of Arunachal Pradesh in addition to study the chemical composition and the related health benefits if any. Methods: Field survey and interview were used for ethnobotany and GCMS was used in the studies of chemical compositions. Results: This study reports numbers of volatile and non-volatile compounds from the fruit of Livistona jenkinsiana with various health benefits. Presence of remarkable high percentage of trehalose sugar is reported in this work from the fruit of L. jenkinsiana. The study also reports various uses of the parts of this palm tree as thatching tree, fencing and house construction. The study also reports need of conservation of this bioculturally important endangered and threatened thatching palm tree. Conclusion: L. jenkinsiana is a bioculturally and economically important endangered thatching palm tree. The fruit could be a source of plant originated fats and trehalose sugar. Fifty percent of compounds present in this fruit are useful in various health problems. There should be further scientific studies in the domains of industrial utility of this underutilized palm tree.

}, keywords = {Biocultural, Ethnobotany, Fruit, Himalayas, Indigenous People, Phytochemical, Thatch, Trehalose}, doi = {xx10.5530/pj.2018.6.206}, author = {Temin Payum} }